Parasites, Hosts and Diseases

"ROS"

Original Article

Comparative evaluation of synchronization methods for Plasmodium falciparum: Efficiency, practicality and experimental applicability: Ying Deng, Yi Yin, Yinyue Li, Jiahui Xu, Jin-Hee Han, Yaobao Liu, Feng Lu; Parasites Hosts Dis 2026;64(2):130-140.
Published online April 1, 2026; DOI: https://doi.org/10.3347/PHD.25096

The study of Plasmodium falciparum intraerythrocytic developmental cycle relies on synchronized parasite cultures. This study aimed to quantitatively evaluate 4 common synchronization methods for the culture of P. falciparum 3D7, incorporating a novel nanomaterial-based tool to
objective
ly assess late-stage parasite enrichment. The 4 methods including 5% D-sorbitol, isosmotic Percoll-Sorbitol (60% PS), discontinuous Percoll-Sorbitol gradients (70%–40% PS), and the Plasmion method. Synchronization efficiency was assessed through microscopic examination and flow cytometry. In addition to SYBR Green I as the stain for flow cytometry, we innovatively employed 20 nm polystyrene fluorescent microspheres (PFMs20), which bind to parasites following the production of new permeability pathways, to provide an
objective
validating the enrichment of mature stages. The binding rate was defined as the ratio of PFMs20 positivity to SYBR Green I positivity. Microscopic examination of Giemsa-stained blood smears served as the gold standard for assessing the stage-specific profiles achieved by each synchronization method. The 4 methods yielded distinct efficiency profiles: 5% D-sorbitol achieved 88.37% ring-stage purity; 60% PS demonstrated high late-stage enrichment with PFMs20 binding rates reaching 87.47%; 70%–40% PS uniquely enabled visible hemozoin-rich layer separation and showed 88.12% binding efficiency in its schizont-enriched fraction; while Plasmion yielded a binding rate of 73.37% in its late-stage fraction. Our results formulate a clear decision matrix for synchronization protocol selection. The sorbitol method is ideal for basic ring-stage studies, Percoll-based gradients support rigorous stage-specific investigations, and the Plasmion method is well-suited for resource-limited settings. This comparative analysis provides preliminary guidelines for synchronization method selection that require validation in diverse laboratory and field contexts.

346 View
7 Download

Brief Communication

Microscopic findings of host–parasite interaction between the Korean rockfish, Sebastes schlegelii, and the monogenean ectoparasite, Microcotyle sebastis: Sang Phil Shin, Seokryel Kim; Parasites Hosts Dis 2024;62(4):490-496.
Published online November 22, 2024; DOI: https://doi.org/10.3347/PHD.24043

Abstract
PDF

This study was conducted to examine the pathogenesis of gill degeneration in the Korean rockfish Sebastes schlegelii, infested with the monogenean ectoparasite, Microcotyle sebastis. We collected 30 Korean rockfish from a fish farm in Tongyeong-si, Gyeongsangnam-do, and examined them with light microscopy, scanning electron microscopy (SEM), and histopathology, in March 2018. The monogenean trematode, M. sebastis, was detected in 27 Korean rockfish (90%), with the intensity of infection being 31.7 per fish. The characteristic surface ultrastructures such as tegument with transverse striations, genital atrium, genital pore, and opisthaptor with numerous clamps were observed. The worms were firmly attached to the gill lamellae using clamps from the opisthaptor, causing gill damage and degeneration. The distal part of the lamellae was ruptured by the sclerites of the clamps. The histopathological examination revealed epithelial hypertrophy, hyperplasia, and occasionally fusion of the lamellae. These ultrastructural and histopathological findings provide some understanding of the pathogenesis of gill degeneration in the Korean rockfish infested with M. sebastis.

2,701 View
72 Download

Original Articles

A novel kit for enrichment of fecal helminth eggs: Eunsol Lee, Seon-Ok Back, Young-Ju Lee, Jung-Won Ju, Hee-Il Lee, Myoung-Ro Lee; Parasites Hosts Dis 2024;62(3):323-329.
Published online August 26, 2024; DOI: https://doi.org/10.3347/PHD.24021

Abstract
PDF

We developed a new concentration kit, called the ParaEgg (PE), for easy detection trematode eggs from fecal samples in endemic areas of clonorchiasis and metagonimiasis in Korea. To create a standard of detection efficiency, 120 fecal samples were examined using the water–ether concentration method (WECM). The PE kit and Mini ParaSep (PS) kit were used to compare the detection sensitivity of 100 egg-positive and 20 egg-negative samples in WECM. Additionally, stool samples, which were intentionally spiked with 10, 20, and 30 Clonorchis sinensis eggs, were evaluated to assess the sensitivity in lowinfection cases. The PE and PS kits showed detection rates of 100% and 92%, respectively, from 100 egg-positive samples in WECM. Meanwhile, eggs were detected in 3 (PE) and 2 (PS) out of 20 egg-negative samples in WECM. The PE kit detected the highest number of eggs per gram of feces (727 on average), followed by the WECM (524) and PS kit (432). In fecal samples that were intentionally spiked with 10, 20, and 30 C. sinensis eggs, PE only detected eggs 2 out of 5 samples in 10 eggs spiked (40%), and the detection rates were 80% and 100%, respectively. The PE kit enabled a more accurate identification of trematode eggs because of the clearance of small fecal debris in the microscopic field. In conclusion, the PE kit is obviously helpful to detect and identify trematode eggs in stool examinations especially in endemic areas of clonorchiasis and metagonimiasis.

Citations

Citations to this article as recorded by

Artificial Intelligence-Based Detection of Clonorchis sinensis and Metagonimus spp. Eggs Using an Automated Microscope Solution
Hee-Eun Shin, Young-Ju Lee, Seon-Ok Back, Jung-Won Ju, Hee-Il Lee, Mi-Jin Kim, Young-Min Shin, Myoung-Ro Lee
Parasitologia.2026; 6(1): 7. CrossRef
Molecular evidence for human Metagonimus kogai and M. saitoi infection in Korea: Detection of COI genes in the feces of riverside people along the Seomjin‑gang (river)
Eunsol Lee, Jong-Hun Choi, Yeong-Ju Lee, Seon-Ok Baek, Hee-Il Lee, Jung-Won Ju, Myeong-Ro Lee, Tae Yun Kim
Parasites, Hosts and Diseases.2026; 64(2): 123. CrossRef
2024년 장내기생충 유행지역 감염 조사

Public Health Weekly Report.2025; 18(19): 707. CrossRef
Diagnostic performance evaluation of ParaEgg for identifying intestinal helminthiasis: A comparative study with conventional copromicroscopy
Tilak Chandra Nath, Heeil Lee, Md. Mahamudul Hasan, Tanmoy Roy Rudro, Dipta Das, Md. Taufiqur Rahman, Nandiny Saha Roy, Pritha Parial, Proloy Chakraborty Tusher, Tarek Siddiki
Parasite Epidemiology and Control.2025; 30: e00449. CrossRef
Detection of intestinal parasites in leopard cat fecal samples using shotgun metagenomics
Jun Ho Choi, Singeun Oh, Myung-hee Yi, Dongjun Kang, Du-Yeol Choi, Xavier Chavarria, Arwa Shatta, Yoon Hee Cho, Seongjun Choe, Seung-Hun Lee, Ju Yeong Kim
Parasites, Hosts and Diseases.2025; 63(4): 349. CrossRef

5,244 View
95 Download
3 Web of Science
Crossref

Prostaglandin synthase activity of sigma- and mu-class glutathione transferases in a parasitic trematode, Clonorchis sinensis: Jiyoung Kim, Woon-Mok Sohn, Young-An Bae; Parasites Hosts Dis 2024;62(2):205-216.
Published online May 27, 2024; DOI: https://doi.org/10.3347/PHD.24004

Sigma-class glutathione transferase (GST) proteins with dual GST and prostaglandin synthase (PGS) activities play a crucial role in the establishment of Clonorchis sinensis infection. Herein, we analyzed the structural and enzymatic properties of sigma-class GST (CsGST-σ) proteins to obtain insight into their antioxidant and immunomodulatory functions in comparison with mu-class GST (CsGST-μ) proteins. CsGST-σ proteins conserved characteristic structures, which had been described in mammalian hematopoietic prostaglandin D₂ synthases. Recombinant forms of these CsGST-σ and CsGST-μ proteins expressed in Escherichia coli exhibited considerable degrees of GST and PGS activities with substantially different specific activities. All recombinant proteins displayed higher affinities toward prostaglandin H₂ (PGS substrate; average Km of 30.7 and 3.0 μm for prostaglandin D₂ [PGDS] and E₂ synthase [PGES], respectively) than those toward CDNB (GST substrate; average K_m of 1,205.1 μm). Furthermore, the catalytic efficiency (K_cat/K_m) of the PGDS/PGES activity was higher than that of GST activity (average K_cat/K_m of 3.1, 0.7, and 7.0×10^-3 s^-1μm^-1 for PGDS, PGES, and GST, respectively). Our data strongly suggest that the C. sinensis sigma- and mu-class GST proteins are deeply involved in regulating host immune responses by generating PGD₂ and PGE₂ in addition to their roles in general detoxification.

3,792 View
59 Download

Involvement of NOX2-derived ROS in human hepatoma HepG2 cell death induced by Entamoeba histolytica: Young Ah Lee, Myeong Heon Shin; Parasites Hosts Dis 2023;61(4):388-396.
Published online November 28, 2023; DOI: https://doi.org/10.3347/PHD.23094

Abstract
PDF

Entamoeba histolytica is an enteric tissue-invasive protozoan parasite causing amoebic colitis and liver abscesses in humans. Amoebic contact with host cells activates intracellular signaling pathways that lead to host cell death via generation of caspase-3, calpain, Ca²⁺ elevation, and reactive oxygen species (ROS). We previously reported that various NADPH oxidases (NOXs) are responsible for ROS-dependent death of various host cells induced by amoeba. In the present study, we investigated the specific NOX isoform involved in ROS-dependent death of hepatocytes induced by amoebas. Co-incubation of hepatoma HepG2 cells with live amoebic trophozoites resulted in remarkably increased DNA fragmentation compared to cells incubated with medium alone. HepG2 cells that adhered to amoebic trophozoites showed strong dichlorodihydrofluorescein diacetate (DCF-DA) fluorescence, suggesting intracellular ROS accumulation within host cells stimulated by amoebic trophozoites. Pretreatment of HepG2 cells with the general NOX inhibitor DPI or NOX2-specific inhibitor GSK 2795039 reduced Entamoeba-induced ROS generation. Similarly, Entamoeba-induced LDH release from HepG2 cells was effectively inhibited by pretreatment with DPI or GSK 2795039. In NOX2-silenced HepG2 cells, Entamoeba-induced LDH release was also significantly inhibited compared with controls. Taken together, the results support an important role of NOX2-derived ROS in hepatocyte death induced by E. histolytica.

Citations

Citations to this article as recorded by

CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2024; 62(3): 270. CrossRef
Optimization of 18 S rRNA metabarcoding for the simultaneous diagnosis of intestinal parasites
Dongjun Kang, Jun Ho Choi, Myungjun Kim, Sohyeon Yun, Singeun Oh, Myung-hee Yi, Tai-Soon Yong, Young Ah Lee, Myeong Heon Shin, Ju Yeong Kim
Scientific Reports.2024;[Epub] CrossRef

4,479 View
160 Download
2 Web of Science
Crossref

Mini Review

Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia: Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu; Parasites Hosts Dis 2023;61(1):2-14.
Published online February 22, 2023; DOI: https://doi.org/10.3347/PHD.22160

Abstract
PDF

Trichomonas vaginalis is a flagellated protozoan that causes trichomoniasis, a common nonviral sexually transmitted infection. T. vaginalis infection is asymptomatic in most infected men but can lead to chronic infection. The inflammatory response to chronic T. vaginalis infection may contribute to prostatic diseases, such as prostatitis and benign prostatic hyperplasia (BPH); however, studies on the relationship between T. vaginalis infection and prostate diseases are scarce. In this review, we discuss evidence from our studies on the involvement of T. vaginalis in the pathogenesis of prostate diseases, such as prostatitis and BPH. Studies of prostatitis have demonstrated that the attachment of T. vaginalis trophozoite to prostate epithelial cells (PECs) induces inflammatory cytokine production and inflammatory cell migration, leading to prostatitis. T. vaginalis also causes pathological changes, such as inflammatory cell infiltration, acinar changes, interstitial fibrosis, and mast cell infiltration, in prostate tissues of infected rats. Thus, T. vaginalis is considered an infectious agent that triggers prostatitis. Meanwhile, studies of prostatic hyperplasia revealed that mast cells activated by T. vaginalis-infected prostate cells secreted inflammatory mediators, such as β-hexosaminidase and tryptase, which promoted proliferation of prostate stromal cell (PSC). Moreover, interleukin-6 produced by proliferating PSCs induced the multiplication of BPH-1 epithelial cells as a result of stromal–epithelial interaction, suggesting that the proliferation of T. vaginalis-infected prostate cells can be induced through crosstalk with mast cells. These collective findings suggest that T. vaginalis contributes to the progression of prostatitis and prostatic hyperplasia by creating an inflammatory microenvironment involving PECs and PSCs.

Citations

Citations to this article as recorded by

Epithelial redox stress programs macrophage immunometabolism through a ZNF24-MIF–NF–κB pathway in chronic nonbacterial prostatitis
Fei Zhang, Andong Zhang, Tong Meng, Xianhong Liu, Cheng Yang, Chaozhao Liang, Meng Zhang
Redox Biology.2026; 90: 104042. CrossRef
Immunomodulatory roles of autophagic flux and IFIT in human ectocervical cells upon Trichomonas vaginalis infection
Ching-Chun Liu, Lichieh Julie Chu, Yuan-Ming Yeh, Hsin-Chung Lin, Lih-Chyang Chen, Ching-Yun Huang, Shu-Fang Chiu, Fang-Wen Cheng, Wei-Ning Lin, Kuo-Yang Huang
International Immunopharmacology.2025; 155: 114643. CrossRef
Detection of trichomonads in induced sputum from asthma patients in Korea
Myung-hee Yi, Myungjun Kim, Jun Ho Choi, Yoon Hee Cho, Hyun Kyung Oh, Jung-Won Park, Ju Yeong Kim
Parasites, Hosts and Diseases.2025; 63(3): 254. CrossRef
Interplay of obesity and parasitic infection: current evidence of immunogenesis, tumorigenesis and leptin receptor involvement
Enas El Saftawy, Mansour Alghamdi, Basma Emad Aboulhoda
Nutrition & Metabolism.2025;[Epub] CrossRef
The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis
Alexandra Ibáñez-Escribano, Juan José Nogal-Ruiz
Pathogens.2024; 13(2): 126. CrossRef
Evaluation of the clinical application value of cytokine expression profiles in the differential diagnosis of prostate cancer
Rongfa Chen, Linna Liu, Hui Chen, Chao Xing, Tingting Zhang, Yilin Pang, Xunjun Yang
Cancer Immunology, Immunotherapy.2024;[Epub] CrossRef
CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2024; 62(3): 270. CrossRef
Dynamin 2-mediated endocytosis of BLT1 is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2024; 62(3): 281. CrossRef

10,720 View
299 Download
9 Web of Science
Crossref

Original Article

Malaria Endemicity in the Rural Communities of Ebonyi State, Nigeria: David Ekene Nwele, Ikechukwu Oliver Onyali, Milliam Okwudili Iwueze, Michael Okpara Elom, Ogbonna Elom Sabastian Uguru; Korean J Parasitol 2022;60(3):173-179.
Published online June 30, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.3.173

Abstract
PDF

Malaria remains a global health threat. Approximately 97% of the population is at risk in sub-Saharan countries, particularly Nigeria. This study compared the performance of 2 diagnostic methods in assessing malaria endemicity in the rural communities of Ebonyi State, Nigeria. A total of 1,140 study participants were screened for malaria parasite using Rapid Diagnostic Test kits (RDT) in the field, while thick and thin films for microscopy were examined in the laboratory. Our result showed that malaria prevalence was 56.8 by RDT and 38.6% by microscopic test. Age group under 10 years had the highest prevalence of 28.9% (RDT) and 23.6% (microscopy), respectively. The highest prevalence of 19.5% by RDT was recorded in Onicha Local Government Area, while the highest prevalence of 13.4% with microscopy was recorded in Ezza North Local Government Area. The sensitivity and specificity of microscopic examination were both 100%, while those of RDT were 95.5% and 75.9%, respectively.

Citations

Citations to this article as recorded by

Subclinical Plasmodium spp. Infections in a Community Setting in Bangui, Central African Republic
Romaric Nzoumbou-Boko, Mireille Cornelia Ingrid Denissio Morissi Nalingbo, Brice Martial Yambiyo, Roger Detol, Ermeland Moussa, Didita Nalinga, Lydie Joella-Venus de la Grace Namsenei-Dankpea, Alexandre Manirakiza, Lawrence Ayong, Yap Boum
Research and Reports in Tropical Medicine.2025; Volume 16: 1. CrossRef
Explainable AI for enhanced accuracy in malaria diagnosis using ensemble machine learning models
Olushina Olawale Awe, Peter Njoroge Mwangi, Samuel Kotva Goudoungou, Ruth Victoria Esho, Olanrewaju Samuel Oyejide
BMC Medical Informatics and Decision Making.2025;[Epub] CrossRef
Ectoparasite species diversity and prevalence in pigs (Sus scrofa domesticus) within delta central senatorial district, Delta State, Nigeria

International Journal of Biosciences (IJB).2025; : 320. CrossRef
Analysis of fractional-order model for the transmission dynamics of malaria via Caputo–Fabrizio and Atangana–Baleanu operators
Benedict Celestine Agbata, Raimonda Dervishi, Mehmet Gümüş, Aseel Smerat, Godwin Christopher Ezike Mbah
Scientific Reports.2025;[Epub] CrossRef
Prevalence of Malaria Parasites among Febrile Patients in a Teaching Hospital in South East Nigeria
Felix Edoiseh Ehidiamhen, Ruth Chinenye Ibegbulem, Idakari Chinedu Nweke, Comfort Nne Akujobi, Aja Josiah Nwandibia, Anemeje Onyeka Afam, Dafup Katdel Istifanus, Kenneth Eze Etu, Elom Onochie Peter, Onwe Francis Idenyi, Robinson Uchenna Ugwuanyi, Chinyere
Advances in Infectious Diseases.2024; 14(04): 873. CrossRef
Estimated distribution of malaria cases among children in sub-Saharan Africa by specified age categories using data from the Global Burden of Diseases 2019
Olorunfemi A. Oshagbemi, Pedro Lopez-Romero, Cornelis Winnips, Katalin R. Csermak, Guoqin Su, Elodie Aubrun
Malaria Journal.2023;[Epub] CrossRef

6,208 View
174 Download
4 Web of Science
Crossref

Mini Review

Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica: Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin; Korean J Parasitol 2022;60(3):155-161.
Published online June 30, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.3.155

Abstract
PDF

All living organisms are destined to die. Cells, the core of those living creatures, move toward the irresistible direction of death. The question of how to die is critical and is very interesting. There are various types of death in life, including natural death, accidental death, questionable death, suicide, and homicide. The mechanisms and molecules involved in cell death also differ depending on the type of death. The dysenteric amoeba, E. histolytica, designated by the German zoologist Fritz Schaudinn in 1903, has the meaning of tissue lysis; i.e., tissue destroying, in its name. It was initially thought that the amoebae lyse tissue very quickly leading to cell death called necrosis. However, advances in measuring cell death have allowed us to more clearly investigate the various forms of cell death induced by amoeba. Increasing evidence has shown that E. histolytica can cause host cell death through induction of various intracellular signaling pathways. Understanding of the mechanisms and signaling molecules involved in host cell death induced by amoeba can provide new insights on the tissue pathology and parasitism in human amoebiasis. In this review, we emphasized on the signaling role of NADPH oxidases in reactive oxygen species (ROS)-dependent cell death by pathogenic E. histolytica.

Citations

Citations to this article as recorded by

Involvement of NOX2-derived ROS in human hepatoma HepG2 cell death induced by Entamoeba histolytica
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2023; 61(4): 388. CrossRef

5,590 View
185 Download
1 Web of Science
Crossref

Original Articles

Involvement of Macrophages in Proliferation of Prostate Cancer Cells Infected with Trichomonas vaginalis: Kyu-Shik Kim, Hong-Sang Moon, Sang-Su Kim, Jae-Sook Ryu; Korean J Parasitol 2021;59(6):557-564.
Published online December 22, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.6.557

Abstract
PDF

Macrophages play a key role in chronic inflammation, and are the most abundant immune cells in the tumor microenvironment. We investigated whether an interaction between inflamed prostate cancer cells stimulated with Trichomonas vaginalis and macrophages stimulates the proliferation of the cancer cells. Conditioned medium was prepared from T. vaginalis-infected (TCM) and uninfected (CM) mouse prostate cancer (PCa) cell line (TRAMP-C2 cells). Thereafter conditioned medium was prepared from macrophages (J774A.1 cell line) after incubation with CM (MCM) or TCM (MTCM). When TRAMP-C2 cells were stimulated with T. vaginalis, protein and mRNA levels of CXCL1 and CCL2 increased, and migration of macrophages toward TCM was more extensive than towards CM. Macrophages stimulated with TCM produced higher levels of CCL2, IL-6, TNF-α, their mRNAs than macrophages stimulated with CM. MTCM stimulated the proliferation and invasiveness of TRAMP-C2 cells as well as the expression of cytokine receptors (CCR2, GP130, CXCR2). Importantly, blocking of each cytokine receptors with anti-cytokine receptor antibody significantly reduced the proliferation and invasiveness of TRAMP-C2 cells. We conclude that inflammatory mediators released by TRAMP-C2 cells in response to infection by T. vaginalis stimulate the migration and activation of macrophages and the activated macrophages stimulate the proliferation and invasiveness of the TRAMP-C2 cells via cytokine-cytokine receptor binding. Our results therefore suggested that macrophages contribute to the exacerbation of PCa due to inflammation of prostate cancer cells reacted with T. vaginalis.

Citations

Citations to this article as recorded by

IKK2, calcium, MAP kinase, and PI3 kinase are required for exocytosis and interleukin-8 production in human mast cells stimulated by Trichomonas vaginalis-derived secretory products
Shin Hye Park, Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2026; 64(1): 45. CrossRef
The role of proinflammatory cytokines and CXC chemokines (CXCL1–CXCL16) in the progression of prostate cancer: insights on their therapeutic management
Amin Ullah, Wang Jiao, Bairong Shen
Cellular & Molecular Biology Letters.2024;[Epub] CrossRef
CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2024; 62(3): 270. CrossRef
Point-of-Care Diagnostic for Trichomonas vaginalis, the Most Prevalent, Non-Viral Sexually Transmitted Infection
John F. Alderete, Hermes Chan
Pathogens.2023; 12(1): 77. CrossRef

5,184 View
127 Download
6 Web of Science
Crossref

Signaling Role of Adipocyte Leptin in Prostate Cell Proliferation Induced by Trichomonas vaginalis: Jung-Hyun Kim, Ik-Hwan Han, Su-Jin Shin, Sung-Yul Park, Hyo-Yeoung Chung, Jae-Sook Ryu; Korean J Parasitol 2021;59(3):235-249.
Published online June 21, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.3.235

Abstract
PDF

Leptin is a type of adipokine mainly produced by adipocytes and reported to be overproduced in prostate cancer. However, it is not known whether it stimulates the proliferation of prostate cells. In this study, we investigated whether benign prostatic hyperplasia epithelial cells (BPH-1 cells) infected with Trichomonas vaginalis induced the proliferation of prostate cells via a leptin signaling pathway. To investigate the effect of crosstalk between adipocyte leptin and inflamed epithelial cell in proliferation of prostate cells, adipocytes 3T3-L1 cells were incubated in conditioned medium of BPH-1 cells infected with T. vaginalis (T. vaginalis-conditioned medium, TCM), and then the adipocyte-conditioned medium (ATCM) was identified to cause proliferation of prostate cells. BPH-1 cells incubated with live T. vaginalis released pro-inflammatory cytokines, and conditioned medium of these cells caused migration of adipocytes. When prostate stromal cells and BPH-1 cells were incubated with adipocyte conditioned medium containing leptin, their growth rates increased as did expression of the leptin receptor (known as OBR) and signaling molecules such as JAK2/STAT3, Notch and survivin. Moreover, blocking the OBR reduced this proliferation and the expression of leptin signaling molecules in response to ATCM. In conclusion, our findings show that inflamed BPH-1 cells infected with T. vaginalis induce the proliferation of prostate cells through leptin-OBR signaling. Therefore, it is likely that T. vaginalis contributes to prostate enlargement in BPH via adipocyte leptin released as a result of inflammation of the prostate.

Citations

Citations to this article as recorded by

Interplay of obesity and parasitic infection: current evidence of immunogenesis, tumorigenesis and leptin receptor involvement
Enas El Saftawy, Mansour Alghamdi, Basma Emad Aboulhoda
Nutrition & Metabolism.2025;[Epub] CrossRef
Intrinsic and extrinsic factors causing hyperplasia of the prostate
Yuki Kyoda, Kosuke Shibamori, Tetsuya Shindo, Takeshi Maehana, Kohei Hashimoto, Ko Kobayashi, Toshiaki Tanaka, Fumimasa Fukuta, Naoya Masumori
International Journal of Urology.2024; 31(7): 705. CrossRef
Association between metabolic syndrome and benign prostatic hyperplasia: The underlying molecular connection
Xun Fu, Yutao Wang, Yi Lu, Jiang Liu, Hongjun Li
Life Sciences.2024; 358: 123192. CrossRef
Point-of-Care Diagnostic for Trichomonas vaginalis, the Most Prevalent, Non-Viral Sexually Transmitted Infection
John F. Alderete, Hermes Chan
Pathogens.2023; 12(1): 77. CrossRef
Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility
Zhenchao Zhang, Fakun Li, Yangyang Deng, Yuhua Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lihua Guo, Lixia Hao, Xuefang Mei
Acta Tropica.2023; 238: 106794. CrossRef
The correlation between Trichomonas vaginalis infection and reproductive system cancer: a systematic review and meta-analysis
Zhenchao Zhang, Dongxian Li, Yuhua Li, Rui Zhang, Xianghuan Xie, Yi Yao, Linfei Zhao, Xiaowei Tian, Zhenke Yang, Shuai Wang, Xuejing Yue, Xuefang Mei
Infectious Agents and Cancer.2023;[Epub] CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Proliferation of Mouse Prostate Cancer Cells Inflamed by Trichomonas vaginalis
Sang-Su Kim, Kyu-Shik Kim, Ik-Hwan Han, Yeseul Kim, Seong Sik Bang, Jung-Hyun Kim, Yong-Suk Kim, Soo-Yeon Choi, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(6): 547. CrossRef
Involvement of Macrophages in Proliferation of Prostate Cancer Cells Infected with Trichomonas vaginalis
Kyu-Shik Kim, Hong-Sang Moon, Sang-Su Kim, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(6): 557. CrossRef

6,571 View
96 Download
9 Web of Science
Crossref

Case Reports

First Clinical Cases of Spirometrosis in Two Cats in Korea: Joohyung Kim, Younsung Ock, Kihwan Yang, Seongjun Choe, Kyung-Mee Park, Wan-Kyu Lee, Kyung-Chul Choi, Soochong Kim, Dongmi Kwak, Seung-Hun Lee; Korean J Parasitol 2021;59(2):153-157.
Published online April 22, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.2.153

This study reports the first two clinical cases of spirometrosis caused by Spirometra sp. in cats in Korea. In these two cases, the cats vomited, and long proglottids of tapeworm were recovered. The sick cats presented with anorexia and lethargy. However, they unexpectedly showed no diarrhea, which is the main symptom of spirometrosis. Based on a fecal floatation test as well as morphological and molecular analyses, the parasite was diagnosed as Spirometra sp. The 2 cases were treated with praziquantel. This study suggests regular monitoring of health and deworming in companion animals, even when animals are well cared for, with regular preventive medication. Additionally, spirometrosis should be considered in the differential diagnosis in cases of gastrointestinal symptoms in Spirometra endemic areas.

Citations

Citations to this article as recorded by

First morphological and molecular characterisation of Spirometra mansoni (Cestoda, Diphyllobothriidae) in a domestic cat from Veracruz, Mexico
Enrique Salazar-Grosskelwing, Roger I. Rodriguez-Vivas, Manuel E. Bolio-González, Dora Romero-Salas, Rodolfo Ramos-Beltrán, Alberto Solano-Barquero, Alicia Rojas
Veterinary Parasitology: Regional Studies and Reports.2025; 57: 101189. CrossRef
Description of Spirometra asiana sp. nov. (Cestoda: Diphyllobothriidae) found in wild boars and hound dogs in Japan
Hiroshi Yamasaki, Hiromu Sugiyama, Yasuyuki Morishima, Hirotaka Kobayashi
Parasitology International.2024; 98: 102798. CrossRef
Molecular identification of Spirometra infections in companion animals and wildlife in Japan
Hiroshi YAMASAKI, Hiromu SUGIYAMA, Yasuyuki MORISHIMA, Yasuhito SAKO
Journal of Veterinary Medical Science.2024; 86(4): 409. CrossRef
WITHDRAWN: First morphological and molecular characterisation of Spirometra mansoni (Cestoda, Diphyllobothriidae) in a domestic cat from Veracruz, Mexico
Enrique Salazar-Grosskelwing, Roger I. Rodriguez-Vivas, Manuel E. Bolio-González, Dora Romero-Salas, Rodolfo Ramos-Beltrán, Alberto Solano-Barquero, Alicia Rojas
Veterinary Parasitology: Regional Studies and Reports.2024; 56: 101127. CrossRef

5,782 View
139 Download
5 Web of Science
Crossref

Cerebral Coenurosis of a Long-Tailed Goral, Naemorhedus caudatus, in Korea: Sangjin Ahn, Hyeongseok Oh, Soo-Young Choi, Jong-Taek Kim, Hyeon-Cheol Kim; Korean J Parasitol 2021;59(1):55-59.
Published online February 19, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.1.55

Abstract
PDF

We intended to describe a case of cerebral coenurosis in a long-tailed goral, Naemorhedus caudatus, from Hwacheon-gun, Gangwon-do (Province), in the Korea. The goral, a 10-year-old male, was suffering from neurological symptoms, such as turning the circle to one side without lifting the head straight, and died at 30 days after admission to the wildlife medical rescue center in Chuncheon-si, Gangwon-do. A fluid-filled cyst was detected in the left cerebral hemisphere by computed tomography and magnetic resonance imaging. The cyst removed from the deceased goral was transparent, about 3×3 cm in size, contained a clear fluid and approximately 320 protoscolices invaginating from the internal germinal layer. The protoscolex had 4 suckers and a rostellum with 28 hooklets arranged in 2 rows. By the present study, a case of cerebral coenurosis was first confirmed in a long-tailed goral, N. caudatus, from Gangwon-do, in Korea. The residents frequently exposed in the sylvatic environment should be careful the accidental infections of zoonotic metacestode of Taenia multiceps, Coenurus cerebralis, in Korea.

Citations

Citations to this article as recorded by

Radiographic Evaluation of Vertebral Heart Scale and Caudal Vena Cava Size Useful for Diagnosing Cardiac Diseases in Endangered Long-Tailed Goral (Naemorhedus caudatus)
Sangjin Ahn, Woojin Shin, Yujin Han, Sohwon Bae, Chea-Un Cho, Sooyoung Choi, Jong-Taek Kim
Journal of Veterinary Clinics.2023; 40(2): 119. CrossRef
ОПТИМИЗАЦИЯ УСЛОВИЙ ЭКСПРЕССИИ РЕКОМБИНАНТНЫХ АНТИГЕНОВ-КАНДИДАТОВ ВАКЦИН ПРОТИВ ЦЕНУРОЗА
Е. А. Колосова, П. В. Колосов, Ю. А. Меркульева, Д. Н. Щербаков
Актуальная биотехнология.2023; (1): 57. CrossRef
Successful treatment of gastrocnemius muscle rupture in a long-tailed goral rescued from a live trap: a case report
Sangjin Ahn, Sooyoung Choi, Jong-Taek Kim
Journal of Veterinary Science.2022;[Epub] CrossRef

11,631 View
112 Download
1 Web of Science
Crossref

Original Articles

Morphological and Molecular Characterization of Toxocara apodemi (Nematoda: Ascarididae) from Striped Field Mice, Apodemus agrarius, in Korea: Hyeon Cheol Kim, Eui Ju Hong, Si Yun Ryu, Jinho Park, Jeong Gon Cho, Do Hyeon Yu, Joon Seok Chae, Kyoung Seong Choi, Bae Keun Park; Korean J Parasitol 2020;58(4):403-411.
Published online August 25, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.4.403

Abstract
PDF

Adult ascarid worms from the field mice, Apodemus agrarius, were observed with a light and scanning electron microscope, and molecularly analized with 18S rRNA gene. In the scanning electron microscope, 3 prominent labia were present in the anterior end of male and female worms, but the interlabia and gubernaculum were absent. Scanning electron micrographs showed cervical alae as vestigial organs that looked like a slightly uplifted superficial sewing stitch. Total 6 pairs of post-cloacal papillae were observed on the tail of the male worms. The tail of female worms was blunt and conical shape with a spine-like structure, mucron. The eggs were sub-globular, coated with the albuminous layer and 73 by 82 μm in average size. The superficial pits of T. apodemi egg (mean 8.6×6.7 μm) are obviously bigger than those of Toxocara spp. The partial sequence of 18S rRNA showed the sequence homology of Toxocara canis (99.6%), Toxocara cati (99.4%), Toxascaris leonina (99.4%), and Toxocara vitulorum (99.2%). Conclusively, it was confirmed that ascarid nematodes, Toxocara apodemi, recovered from striped field mice in Korea are taxonomically conspecific relationship with genus Toxocara and genetic divergence from other Toxocara species.

Citations

Citations to this article as recorded by

First molecular characterization and species validation of Toxocara alienata (Rudolphi 1819) (Nematoda: Ascarididae), from a crab-eating raccoon (Procyon cancrivorus)
Gustavo Macêdo do Carmo, Lorena Gisela Ailán-Choke, Hassan Jerdy, Hermínio Ismael de Araújo-Júnior, Felipe Bisaggio Pereira
Parasitology International.2026; 111: 103160. CrossRef
Parasites in the Digestive Tract of Lion (Panthera leo) at the Safari Park of Gurun Putih Lestari Jantho, Aceh Besar
Fajri Dwitama, Razali Daud, Arman Sayuti, Yudha Fahrimal
Journal of Applied Veterinary Science And Technology.2025; 6(1): 32. CrossRef
Nematocidal activity of zinc oxide nanoparticles synthesized using chicken egg albumin in lung and spleen of Parascaris equorum experimentally infected rats
Sara Bayoumi Ali, Ayman Saber Mohamed, Sohair R. Fahmy, Manal El–Garhy, Mohamed R. Mousa, Fathy Abdel-Ghaffar
Scientific Reports.2025;[Epub] CrossRef
Toxocariasis: An Overlooked Neglected Tropical Disease in Latin America
Alfonso J. Rodriguez-Morales, Ivan Camilo Jaramillo-Montoya, Jessica Marcela Hermosa-Sanchez, Juan Felipe López-Marín, Maria Camila Bernal-Chica, Nahun Alejandro Giraldo-Corrales, Omar Jair Mantilla-Moreno, Hector Andres Mosquera-Alba, Sergio Imbachi-Anac
Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo.2025; 18(1): e2835. CrossRef
Incidence and Risk Factors of Toxocara vitulorum Infection in Beef Cattle of Yogyakarta, Indonesia
Vika Ichsania Ninditya, Fitrine Ekawasti, Joko Prastowo, Irkham Widiyono, Wisnu Nurcahyo
World's Veterinary Journal.2024; 14(4): 592. CrossRef
Characterisation of the mitochondrial genome and phylogenetic analysis of Toxocara apodemi (Nematoda: Ascarididae)
Y. Gao, Y. Hu, S. Xu, H. Liang, H. Lin, T. H. Yin, K. Zhao
Journal of Helminthology.2024;[Epub] CrossRef
The Complete Mitogenome of Toxocara vitulorum: Novel In-Sights into the Phylogenetics in Toxocaridae
Yue Xie, Lidan Wang, Yijun Chen, Zhao Wang, Pengchen Zhu, Zun Hu, Xinfeng Han, Zhisheng Wang, Xuan Zhou, Zhicai Zuo
Animals.2022; 12(24): 3546. CrossRef

9,984 View
204 Download
5 Web of Science
Crossref

Spatiotemporal Trends of Malaria in Relation to Economic Development and Cross-Border Movement along the China?Myanmar Border in Yunnan Province: Xiaotao Zhao, Weerapong Thanapongtharm, Siam Lawawirojwong, Chun Wei, Yerong Tang, Yaowu Zhou, Xiaodong Sun, Jestumon Sattabongkot, Jaranit Kaewkungwal; Korean J Parasitol 2020;58(3):267-278.
Published online June 26, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.3.267

Abstract
PDF

The heterogeneity and complexity of malaria involves political and natural environments, socioeconomic development, cross-border movement, and vector biology; factors that cannot be changed in a short time. This study aimed to assess the impact of economic growth and cross-border movement, toward elimination of malaria in Yunnan Province during its pre-elimination phase. Malaria data during 2011-2016 were extracted from 18 counties of Yunnan and from 7 villages, 11 displaced person camps of the Kachin Special Region II of Myanmar. Data of per-capita gross domestic product (GDP) were obtained from Yunnan Bureau of Statistics. Data were analyzed and mapped to determine spatiotemporal heterogeneity at county and village levels. There were a total 2,117 malaria cases with 85.2% imported cases; most imported cases came from Myanmar (78.5%). Along the demarcation line, malaria incidence rates in villages/camps in Myanmar were significantly higher than those of the neighboring villages in China. The spatial and temporal trends suggested that increasing per-capita GDP may have an indirect effect on the reduction of malaria cases when observed at macro level; however, malaria persists owing to complex, multi-faceted factors including poverty at individual level and cross-border movement of the workforce. In moving toward malaria elimination, despite economic growth, cooperative efforts with neighboring countries are critical to interrupt local transmission and prevent reintroduction of malaria via imported cases. Cross-border workers should be educated in preventive measures through effective behavior change communication, and investment is needed in active surveillance systems and novel diagnostic and treatment services during the elimination phase.

Citations

Citations to this article as recorded by

Plasmodium falciparum genetic diversity in border areas of Myanmar assessed by genotyping merozoite surface protein genes
Jia Ma, Yanrui Wu, Weilin Zeng, Xi Chen, Zheng Xiang, Cuiying Li, Yanmei Zhang, Hui Zhao, Mengxi Duan, Tao Liang, Ye Mou, Xiuya Tang, Cheng Liu, Myat Thurein Kyaw, Aye Nyein Thu, Myat Phone Kyaw, Liwang Cui, Zhaoqing Yang
Parasite Epidemiology and Control.2026; : e00489. CrossRef
Estimating the burden of imported malaria in the Asia Pacific region: a systematic review and meta-analysis
Kinley Penjor, Binay Thapa, Tsheten Tsheten, Soumya Mazumdar, Nasser Bagheri, Manas Kotepui, Kinley Wangdi
Journal of Travel Medicine.2026;[Epub] CrossRef
Molecular epidemiology of Clostridioides difficile in border areas of Yunnan Province suggests possible transmission routes of the strains
Wenpeng Gu, Jiao Gong, Junrong Liang, Xiaofang Zhou, Lulu Bai, Wenzhu Zhang, Senquan Jia, Yongming Zhou, Xiaoqing Fu, Yuan Wu
Infection, Genetics and Evolution.2025; 136: 105860. CrossRef
Understanding the impact of mobility on Plasmodium spp. carriage in an Amazon cross-border area with low transmission rate
Hélène Tréhard, Lise Musset, Yassamine Lazrek, Felix Djossou, Loïc Epelboin, Emmanuel Roux, Jordi Landier, Jean Gaudart, Emilie Mosnier, André Machado Siqueira
PLOS Global Public Health.2024; 4(2): e0002706. CrossRef
Malaria epidemiology, surveillance and response for elimination in Lao PDR
Chawarat Rotejanaprasert, Vilayvone Malaphone, Mayfong Mayxay, Keobouphaphone Chindavongsa, Virasack Banouvong, Boualam Khamlome, Phoutnalong Vilay, Viengxay Vanisavaeth, Richard J Maude
Infectious Diseases of Poverty.2024;[Epub] CrossRef
A time-series meta-transcriptomic analysis reveals the seasonal, host, and gender structure of mosquito viromes
Yun Feng, Qin-yu Gou, Wei-hong Yang, Wei-chen Wu, Juan Wang, Edward C Holmes, Guodong Liang, Mang Shi
Virus Evolution.2022;[Epub] CrossRef
Governance Quality, Public Health, Education, and Innovation: Study for Novel Implications
Ning Wu
Frontiers in Public Health.2022;[Epub] CrossRef
Effectiveness of indoor residual spraying on malaria control: a systematic review and meta-analysis
Yiguo Zhou, Wan-Xue Zhang, Elijah Tembo, Ming-Zhu Xie, Shan-Shan Zhang, Xin-Rui Wang, Ting-Ting Wei, Xin Feng, Yi-Lin Zhang, Juan Du, Ya-Qiong Liu, Xuan Zhang, Fuqiang Cui, Qing-Bin Lu
Infectious Diseases of Poverty.2022;[Epub] CrossRef
Risk factors associated with malaria infection along China–Myanmar border: a case–control study
Jian-Wei Xu, Dao-Wei Deng, Chun Wei, Xing-Wu Zhou, Jian-Xiong Li
Malaria Journal.2022;[Epub] CrossRef
Malaria Research for Tailored Control and Elimination Strategies in the Greater Mekong Subregion
Jetsumon Sattabongkot, Liwang Cui, Sirasate Bantuchai, Sadudee Chotirat, Jaranit Kaewkungwal, Amnat Khamsiriwatchara, Kirakorn Kiattibutr, Myat Phone Kyaw, Saranath Lawpoolsri, Nay Yi Yi Linn, Lynette Menezes, Jun Miao, Wang Nguitragool, Daniel Parker, Pa
The American Journal of Tropical Medicine and Hygiene.2022; 107(4_Suppl): 152. CrossRef
The economic burden of malaria inpatients and its determinants during China's elimination stage
Fangfei Chen, Xiaoyu Chen, Peng Gu, Xiaodong Sang, Ruijun Wu, Miaomiao Tian, Yisheng Ye, Chengxu Long, Ghose Bishwajit, Lu Ji, Da Feng, Lei Yang, Shangfeng Tang
Frontiers in Public Health.2022;[Epub] CrossRef
From control to elimination: a spatial-temporal analysis of malaria along the China-Myanmar border
Fang Huang, Li Zhang, Jing-Bo Xue, Hong-Ning Zhou, Aung Thi, Jun Zhang, Shui-Sen Zhou, Zhi-Gui Xia, Xiao-Nong Zhou
Infectious Diseases of Poverty.2020;[Epub] CrossRef

7,847 View
118 Download
14 Web of Science
Crossref

The Role of PI3K/AKT Pathway and NADPH Oxidase 4 in Host ROS Manipulation by Toxoplasma gondii: Hei Gwon Choi, Fei-Fei Gao, Wei Zhou, Pu-Reum Sun, Jae-Min Yuk, Young-Ha Lee, Guang-Ho Cha; Korean J Parasitol 2020;58(3):237-247.
Published online June 26, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.3.237

Abstract
PDF

Dendritic cell is one of the first innate immune cell to encounter T. gondii after the parasite crosses the host intestinal epithelium. T. gondii requires intact DC as a carrier to infiltrate into host central nervous system (CNS) without being detected or eliminated by host defense system. The mechanism by which T. gondii avoids innate immune defense of host cell, especially in the dendritic cell is unknown. Therefore, we examined the role of host PI3K/AKT signaling pathway activation by T. gondii in dendritic cell. T. gondii infection or T. gondii excretory/secretory antigen (TgESA) treatment to the murine dendritic cell line DC2.4 induced AKT phosphorylation, and treatment of PI3K inhibitors effectively suppressed the T. gondii proliferation but had no effect on infection rate or invasion rate. Furthermore, it is found that T. gondii or TgESA can reduce H₂O₂-induced intracellular reactive oxygen species (ROS) as well as host endogenous ROS via PI3K/AKT pathway activation. While searching for the main source of the ROS, we found that NADPH oxidase 4 (NOX4) expression was controlled by T. gondii infection or TgESA treatment, which is in correlation with previous observation of the ROS reduction by identical treatments. These findings suggest that the manipulation of the host PI3K/AKT signaling pathway and NOX4 expression is an essential mechanism for the down-regulation of ROS, and therefore, for the survival and the proliferation of T. gondii.

Citations

Citations to this article as recorded by

Ferroptosis modulation by Toxoplasma gondii suppresses sodium iodate–driven age-related macular degeneration
Xin-Cheng Wang, Guan-Hao Hong, Yu-Sun Yun, In-Wook Choi, Jaemin Yuk, Guang-Ho Cha
Genes & Genomics.2026;[Epub] CrossRef
Parasites as Modulators of Angiogenesis: Implications for Vascular Biology and Pathogenesis
Tonathiu Rodríguez, Víctor H. Salazar-Castañón, Luis I. Terrazas, Imelda Juárez-Avelar, Miriam Rodríguez-Sosa
Pathogens.2026; 15(4): 347. CrossRef
Small molecule kinase inhibitor altiratinib inhibits brain cyst forming bradyzoites of Toxoplasma gondii
Yeong Hoon Kim, Hye-Jin Ahn, Hwa Sun Kim, Ho-Woo Nam
Journal of Microbiology.2025; 63(2): e2409001. CrossRef
The role of Nrf2 signaling in parasitic diseases and its therapeutic potential
Mohammadamin Vatankhah, Reza Panahizadeh, Ali Safari, Alireza Ziyabakhsh, Behnam Mohammadi-Ghalehbin, Narges Soozangar, Farhad Jeddi
Heliyon.2024; 10(12): e32459. CrossRef
Brain –cyst-driven genes expression in Toxoplasma Gondii Tehran strain: a parasitic-immunogenicity assessment by dint of RNA-Seq
Marzieh Asadi, Zahra Babaei, Ali Afgar, Mohammad Hossein Banabazi, Naser ZiaAli, Ahmad Daryani, Ehsan Aghajani, Milad Mahdavi, Mohamadreza Attari, Farzaneh Zarrinkar
Veterinary Research Communications.2024; 48(4): 2563. CrossRef
BjussuLAAO-II, an l-amino acid oxidase from Bothrops jararacussu snake venom, impairs Toxoplasma gondii infection in human trophoblast cells and villous explants from the third trimester of pregnancy
Thales Alves de Melo Fernandes, Samuel Cota Teixeira, Tássia Rafaela Costa, Alessandra Monteiro Rosini, Guilherme de Souza, Lorena Polloni, Bellisa de Freitas Barbosa, Marcelo José Barbosa Silva, Eloisa Amália Vieira Ferro, Veridiana de Melo Rodrigues Ávi
Microbes and Infection.2023; 25(6): 105123. CrossRef
Toxoplasma gondii inhibits the expression of autophagy-related genes through AKT-dependent inactivation of the transcription factor FOXO3a
Andres Felipe Diez, Louis-Philippe Leroux, Sophie Chagneau, Alexandra Plouffe, Mackenzie Gold, Visnu Chaparro, Maritza Jaramillo, Anita A. Koshy
mBio.2023;[Epub] CrossRef
Regulation of phosphoinositide metabolism in Apicomplexan parasites
Angela Arabiotorre, Vytas A. Bankaitis, Aby Grabon
Frontiers in Cell and Developmental Biology.2023;[Epub] CrossRef
FAF1 downregulation by Toxoplasma gondii enables host IRF3 mobilization and promotes parasite growth
Fei‐Fei Gao, Juan‐Hua Quan, In‐Wook Choi, Yeon‐Jae Lee, Seul‐Gi Jang, Jae‐Min Yuk, Young‐Ha Lee, Guang‐Ho Cha
Journal of Cellular and Molecular Medicine.2021; 25(19): 9460. CrossRef

7,858 View
180 Download
9 Web of Science
Crossref

Polarization of M2 Macrophages by Interaction between Prostate Cancer Cells Treated with Trichomonas vaginalis and Adipocytes: Hyo-Yeoung Chung, Jung-Hyun Kim, Ik-Hwan Han, Jae-Sook Ryu; Korean J Parasitol 2020;58(3):217-227.
Published online June 26, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.3.217

Abstract
PDF

Trichomonas vaginalis causes inflammation of the prostate and has been detected in tissues of prostate cancers (PCa), prostatitis and benign prostatic hyperplasia. Obesity is a risk factor for PCa and causes a chronic subclinical inflammation. This chronic inflammation further exacerbates adipose tissue inflammation as results of migration and activation of macrophages. Macrophages are the most abundant immune cells in the PCa microenvironment. M2 macrophages, known as Tumor-Associated Macrophages, are involved in increasing cancer malignancy. In this study, conditioned medium (TCM) of PCa cells infected with live trichomonads contained chemokines that stimulated migration of the mouse preadipocytes (3T3-L1 cells). Conditioned medium of adipocytes incubated with TCM (ATCM) contained Th2 cytokines (IL-4, IL-13). Macrophage migration was stimulated by ATCM. In macrophages treated with ATCM, expression of M2 markers increased, while M1 markers decreased. Therefore, it is suggested that ATCM induces polarization of M0 to M2 macrophages. In addition, conditioned medium from the macrophages incubated with ATCM stimulates the proliferation and invasiveness of PCa. Our findings suggest that interaction between inflamed PCa treated with T. vaginalis and adipocytes causes M2 macrophage polarization, so contributing to the progression of PCa.

Citations

Citations to this article as recorded by

Interplay of obesity and parasitic infection: current evidence of immunogenesis, tumorigenesis and leptin receptor involvement
Enas El Saftawy, Mansour Alghamdi, Basma Emad Aboulhoda
Nutrition & Metabolism.2025;[Epub] CrossRef
Adipocyte–Tumor Interactions in the Bone Marrow Niche: Implications for Metastasis and Therapy
Alhomam Dabaliz, Mohammad Nawar Al Hakawati, Najmuddeen Alrashdan, Sarah Alrashdan, Mohamad Bakir, Khalid S. Mohammad
International Journal of Molecular Sciences.2025; 26(19): 9781. CrossRef
Extracellular Vesicles Derived from Three-Dimensional-Cultured Human Umbilical Cord Blood Mesenchymal Stem Cells Prevent Inflammation and Dedifferentiation in Pancreatic Islets
Eunwon Lee, Seungyeon Ha, Gyuri Kim, Jae Hyeon Kim, Sang-Man Jin, Patricia Murray
Stem Cells International.2023; 2023: 1. CrossRef
The correlation between Trichomonas vaginalis infection and reproductive system cancer: a systematic review and meta-analysis
Zhenchao Zhang, Dongxian Li, Yuhua Li, Rui Zhang, Xianghuan Xie, Yi Yao, Linfei Zhao, Xiaowei Tian, Zhenke Yang, Shuai Wang, Xuejing Yue, Xuefang Mei
Infectious Agents and Cancer.2023;[Epub] CrossRef
The Roles of Tumor-Associated Macrophages in Prostate Cancer
Chenglin Han, Yuxuan Deng, Wenchao Xu, Zhuo Liu, Tao Wang, Shaogang Wang, Jihong Liu, Xiaming Liu, Eshan Khan
Journal of Oncology.2022; 2022: 1. CrossRef
Sevoflurane activates the IL-6/HO-1 pathway to promote macrophage M2 polarization and prostate cancer lung metastasis
Fang Yu, Tao Bai
International Immunopharmacology.2022; 113: 109380. CrossRef
Signaling Role of Adipocyte Leptin in Prostate Cell Proliferation Induced by Trichomonas vaginalis
Jung-Hyun Kim, Ik-Hwan Han, Su-Jin Shin, Sung-Yul Park, Hyo-Yeoung Chung, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(3): 235. CrossRef
Proliferation of Mouse Prostate Cancer Cells Inflamed by Trichomonas vaginalis
Sang-Su Kim, Kyu-Shik Kim, Ik-Hwan Han, Yeseul Kim, Seong Sik Bang, Jung-Hyun Kim, Yong-Suk Kim, Soo-Yeon Choi, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(6): 547. CrossRef
Involvement of Macrophages in Proliferation of Prostate Cancer Cells Infected with Trichomonas vaginalis
Kyu-Shik Kim, Hong-Sang Moon, Sang-Su Kim, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(6): 557. CrossRef
Is Trichomonas vaginalis a Risk Factor for Prostate Cancer? A Systematic Review and Meta-analysis
Gianpaolo Perletti, Vittorio Magri, Louise Beckers-Perletti, Alberto Trinchieri, Konstantinos Stamatiou
Hellenic Urology.2021; 33(1): 12. CrossRef

9,548 View
198 Download
10 Web of Science
Crossref

Dientamoeba fragilis Infection in Patients with Digestive and Non-Digestive Symptoms: A Case-Control Study: Yousry A. Hawash, Khadiga A. Ismail, Taisir Saber, Emad M. Eed, Amany S. Khalifa, Khalaf F. Alsharif, Saleh A. Alghamdi; Korean J Parasitol 2020;58(2):129-134.
Published online April 30, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.2.129

In most developing countries, Dientamoeba fragilis infection is an obscure protozoan infection. We aimed to determine a frequency and clinical importance of D. fragilis infection in Taif, Saudi Arabia. A 1-year case control study included patients with gastrointestinal (cases, n=114) or non-gastrointestinal symptoms (controls, n=90). The fecal samples were examined with the classical parasitological methods for intestinal protozoa, and by real time PCR for D. fragilis. The infection by D. fragilis was detected in 5.8% by PCR and in 4.4% patients by microscopy. The infection was identified more in control group (n=9) than in cases (n=3); a sole infection in 11 patients and mixed with Giardia in 1 patient. The other enteric parasites detected were Blastocystis sp. (8.3%), Giardia sp. (5.3%), Cryptosporidium sp. (2.9%), Entamoeba histolytica (1.4%), Entamoeba coli (0.9%) and Hymenolepis nana (0.4%). Our results tend to reinforce the need to increase awareness of D. fragilis infection in Saudi Arabia.

Citations

Citations to this article as recorded by

Dientamoeba fragilis dominance in IBS and Blastocystis sp. in ulcerative colitis
Melis Cengiz, Yunus Emre Beyhan, Yusuf Kayar
Diagnostic Microbiology and Infectious Disease.2026; 115(1): 117283. CrossRef
Traitement des parasitoses intestinales (amœbose exclue)
C. Prieux-Klotz, M.-C. Sabate Ferris, L. Gan, X. Bohand, P. Rey
EMC - Gastro-entérologie.2024; 41(2): 1. CrossRef
The high co-existence rate of Blastocystis and Dientamoeba fragilis in human faecal samples and the analysis of demographic and clinical findings
Erdogan MALATYALİ, İbrahim YILDIZ, Evren TİLEKLİOGLU, Hatice ERTABAKLAR, Sema ERTUĞ
Journal of Health Sciences and Medicine.2021; 4(4): 422. CrossRef
The Coexistence of Blastocystis spp. in Humans, Animals and Environmental Sources from 2010–2021 in Asia
Adedolapo Aminat Rauff-Adedotun, Farah Haziqah Meor Termizi, Nurshafarina Shaari, Ii Li Lee
Biology.2021; 10(10): 990. CrossRef

11,752 View
172 Download
3 Web of Science
Crossref

Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses: Johnica Jo Morrow, Christian Elowsky; Korean J Parasitol 2019;57(6):581-585.
Published online December 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.6.581

Abstract
PDF

Confocal laser scanning microscopy (CLSM) was used to examine archaeoparasitological specimens from coprolites associated with La Cueva de los Muertos Chiquitos (CMC) located near present-day Durango, Mexico. The eggs for 4 different types of parasites recovered from CMC coprolites were imaged using CLSM to assist with identification efforts. While some of the parasite eggs recovered from CMC coprolites were readily identified using standard light microscopy (LM), CLSM provided useful data for more challenging identifications by highlighting subtle morphological features and enhancing visualization of parasite egg anatomy. While other advanced microscopy techniques, such as scanning electron microscopy (SEM), may also detect cryptic identifying characters, CLSM is less destructive to the specimens. Utilizing CLSM allows for subsequent examinations, such as molecular analyses, that cannot be performed following SEM sample preparation and imaging. Furthermore, CLSM detects intrinsic autofluorescence molecules, making improved identification independent of resource and time-intensive protocols. These aspects of CLSM make it an excellent method for assisting in taxonomic identification and for acquiring more detailed images of archaeoparasitological specimens.

Citations

Citations to this article as recorded by

NTDscope: A multi-contrast portable microscope for disease diagnosis
María Díaz de León Derby, Zaina L. Moussa, Carlos F. Ng, Abdul M. Bhuiya, Joana P. Cabrera, Dipayan Banik, Charles B. Delahunt, Matthew D. Keller, Anne-Laure M. Le Ny, Jaime Garcia-Villena, Elena Dacal, David Bermejo-Peláez, Daniel Cuadrado, Miguel Luengo
PLOS Global Public Health.2026; 6(2): e0005937. CrossRef
Label-free spectral confocal reflectance microscopy for ex vivo neuroimaging and neural structure visualization
Reinher Pimentel-Domínguez, Rainald Pablo Ordaz, Abraham J. Cisneros-Mejorado, Rogelio O. Arellano, Remy Avila
Methods.2025; 241: 140. CrossRef
Fluorescence Microscopy with Deep UV, Near UV, and Visible Excitation for In Situ Detection of Microorganisms
Noel Case, Nikki Johnston, Jay Nadeau
Astrobiology.2024; 24(3): 300. CrossRef
Analytical Techniques for the Preservation of Cultural Heritage: Frontiers in Knowledge and Application
Mina Magdy
Critical Reviews in Analytical Chemistry.2022; 52(6): 1171. CrossRef
Paleomicrobiology of the human digestive tract: A review
Riccardo Nodari, Michel Drancourt, Rémi Barbieri
Microbial Pathogenesis.2021; 157: 104972. CrossRef
Exploring confocal laser scanning microscopy (CLSM) and fluorescence staining as a tool for imaging and quantifying traces of marine microbioerosion and their trace‐making microendoliths
Philipp‐Konrad Schätzle, Max Wisshak, Andreas Bick, André Freiwald, Alexander Kieneke
Journal of Microscopy.2021; 284(2): 118. CrossRef

6,537 View
108 Download
6 Web of Science
Crossref

Genetic Diversity of Plasmodium vivax in Clinical Isolates from Southern Thailand using PvMSP1, PvMSP3 (PvMSP3α, PvMSP3β) Genes and Eight Microsatellite Markers: Supinya Thanapongpichat, Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun; Korean J Parasitol 2019;57(5):469-479.
Published online October 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.5.469

Abstract
PDF

Plasmodium vivax is usually considered morbidity in endemic areas of Asia, Central and South America, and some part of Africa. In Thailand, previous studies indicated the genetic diversity of P. vivax in malaria-endemic regions such as the western part of Thailand bordering with Myanmar. The
objective
of the study is to investigate the genetic diversity of P. vivax circulating in Southern Thailand by using 3 antigenic markers and 8 microsatellite markers. Dried blood spots were collected from Chumphon, Phang Nga, Ranong and, Surat Thani provinces of Thailand. By PCR, 3 distinct sizes of PvMSP3α, 2 sizes of PvMSP3β and 2 sizes of PvMSP1 F2 were detected based on the length of PCR products, respectively. PCR/RFLP analyses of these antigen genes revealed high levels of genetic diversity. The genotyping of 8 microsatellite loci showed high genetic diversity as indicated by high alleles per locus and high expected heterozygosity (H_E). The genotyping markers also showed multiple-clones of infection. Mixed genotypes were detected in 4.8% of PvMSP3α, 29.1% in PvMSP3β and 55.3% of microsatellite markers. These results showed that there was high genetic diversity of P. vivax isolated from Southern Thailand, indicating that the genetic diversity of P. vivax in this region was comparable to those observed other areas of Thailand.

Citations

Citations to this article as recorded by

Genetic diversity of Plasmodium vivax and Plasmodium falciparum field isolates from Honduras in the malaria elimination phase
Alejandro Zamora, Alejandra Pinto, Denis Escobar, Hugo O. Valdivia, Lesly Chaver, Gloria Ardón, Erick Carranza, Gustavo Fontecha
Current Research in Parasitology & Vector-Borne Diseases.2025; 7: 100230. CrossRef
Molecular Characterization of Plasmodium Species to Strengthen Malaria Surveillance in Migrant Populations in Honduras
Ashley Godoy, Kevin Euceda, Alejandra Pinto, Hugo O. Valdivia, Lesly Chaver, Gloria Ardon, Gustavo Fontecha
Tropical Medicine and Infectious Disease.2025; 10(10): 292. CrossRef
Genetic Structure of Introduced Plasmodium vivax Malaria Isolates in Greece, 2015–2019
Ioanna Spiliopoulou, Danai Pervanidou, Nikolaos Tegos, Maria Tseroni, Agoritsa Baka, Annita Vakali, Chrisovaladou-Niki Kefaloudi, Vasilios Papavasilopoulos, Anastasia Mpimpa, Eleni Patsoula
Tropical Medicine and Infectious Disease.2024; 9(5): 102. CrossRef
Asymptomatic Malaria Reservoirs in Honduras: A Challenge for Elimination
Sharon Banegas, Denis Escobar, Alejandra Pinto, Marcela Moncada, Gabriela Matamoros, Hugo O. Valdivia, Allan Reyes, Gustavo Fontecha
Pathogens.2024; 13(7): 541. CrossRef
Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP
Kanyanan Kritsiriwuthinan, Warunee Ngrenngarmlert, Rapatbhorn Patrapuvich, Supaksajee Phuagthong, Kantima Choosang, Jianbing Mu
Journal of Tropical Medicine.2023; 2023: 1. CrossRef
Genetic Diversity of Plasmodium vivax Field Isolates from the Thai–Myanmar Border during the Period of 2006–2016
Abdifatah Abdullahi Jalei, Wanna Chaijaroenkul, Kesara Na-Bangchang
Tropical Medicine and Infectious Disease.2023; 8(4): 210. CrossRef
Genetic diversity and molecular evolution of Plasmodium vivax Duffy Binding Protein and Merozoite Surface Protein-1 in northwestern Thailand
Parsakorn Tapaopong, Gustavo da Silva, Sittinont Chainarin, Chayanut Suansomjit, Khajohnpong Manopwisedjaroen, Liwang Cui, Cristian Koepfli, Jetsumon Sattabongkot, Wang Nguitragool
Infection, Genetics and Evolution.2023; 113: 105467. CrossRef
Genetic Diversity of Plasmodium vivax Merozoite Surface Protein-3 Alpha and Beta from Diverse Geographic Areas of Thailand
Jiraporn Kuesap, Kanchana Rungsihirunrat, Wanna Chaijaroenkul, Mathirut Mungthin
Japanese Journal of Infectious Diseases.2022; 75(3): 241. CrossRef
Prevalence of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency among Malaria Patients in Southern Thailand: 8 Years Retrospective Study
Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat
The Korean Journal of Parasitology.2022; 60(1): 15. CrossRef
PvMSP-3α and PvMSP-3β genotyping reveals higher genetic diversity in Plasmodium vivax parasites from migrant workers than residents at the China-Myanmar border
Xiaosong Li, Yao Bai, Yanrui Wu, Weilin Zeng, Zheng Xiang, Hui Zhao, Wei Zhao, Xi Chen, Mengxi Duan, Xun Wang, Wenya Zhu, Kemin Sun, Yiman Wu, Yanmei Zhang, Yucheng Qin, Benjamin M. Rosenthal, Liwang Cui, Zhaoqing Yang
Infection, Genetics and Evolution.2022; 106: 105387. CrossRef
Genetic characterization of Plasmodium vivax isolates from Pakistan using circumsporozoite protein (pvcsp) and merozoite surface protein-1 (pvmsp-1) genes as genetic markers
Zainab Bibi, Anam Fatima, Rehana Rani, Ayesha Maqbool, Samea Khan, Shumaila Naz, Shahid Waseem
Malaria Journal.2021;[Epub] CrossRef

9,811 View
167 Download
12 Web of Science
Crossref

Case Report

Coenurosis of Yak, Bos grunniens, caused by Taenia multiceps: A Case Report with Molecular Identification in Qinghai Tibetan Plateau Area, China: Xue-Yong Zhang, Ying-Na Jian, Hong Duo, Xiu-Ying Shen, Yi-Juan Ma, Yong Fu, Zhi-Hong Guo; Korean J Parasitol 2019;57(4):423-427.
Published online August 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.4.423

Abstract
PDF

Coenurosis is an important zoonotic helminthic disease caused by the larval stage of the tapeworm Taenia multiceps. This parasite typically infects the brain of the intermediate hosts, including sheep, goat, cattle and even humans. We report a case of T. multiceps infection in a yak confirmed by clinical symptoms, morphological characteristics, and molecular and phylogenetic analyses. The coenurus was thin-walled, whitish, and spherical in shape with a diameter of 10 cm. The parasite species was identified as T. multiceps by PCR amplification and sequencing of the 18S rRNA, cox1 and nad1 genes. Three gene sequences all showed high homology (all above 97%) with the reference sequences from different hosts. Moreover, phylogenetic reconstructions with the 3 published Taenia gene sequences confirmed that the Qinghai yak isolate was closely related to T. multiceps. Although there are advanced diagnosis and treatment methods for coenurosis, early infection is difficult to diagnose. Importantly, the findings of yak infection case should not be ignored due to its zoonotic potential.

Citations

Citations to this article as recorded by

Treatment and management of coenurosis by Taenia multiceps: field data from outbreaks in endemic regions and literature review
I. Abbas, C. Tamponi, G. Madau, L. Cavallo, A. Varcasia, A. Scala
Parasites & Vectors.2024;[Epub] CrossRef
Taenia multiceps coenurosis: a review
Antonio Varcasia, Claudia Tamponi, Fahad Ahmed, Maria Grazia Cappai, Francesca Porcu, Naunain Mehmood, Giorgia Dessì, Antonio Scala
Parasites & Vectors.2022;[Epub] CrossRef

9,244 View
109 Download
2 Web of Science
Crossref

Original Article

Malaria Prevalence in a Low Transmission Area, Jazan District of Southwestern Saudi Arabia: Yousry Hawash, Khadiga Ismail, Khalaf Alsharif, Walaa Alsanie; Korean J Parasitol 2019;57(3):233-242.
Published online June 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.3.233

Abstract
PDF

Detailed description of malaria in low transmission areas is crucial for elimination. The current study aimed to provide a comprehensive description for malaria transmission in Jazan, a low transmission district, southwestern Saudi Arabia. Patients at a tertiary care hospital were recruited in our study between August 2016 and September 2018. Malaria diagnosis was performed through a species-specific nested polymerase chain reaction (nested PCR), microscopy and Paramax-3^TM rapid detection test (RDT). Malaria was detected in 30 patients by the PCR, with point prevalence of 10.9%. Of these malaria infections, 80% was imported, 26.6% was asymptomatic and 23.3% was sub-microscopic. Malaria was reported throughout the year, with February/March and September/October peaks. Infection was significantly more in males than in females (P=0.01). Likewise, infections were detected more in febrile than in non-febrile patients (P=0.01). Adult aged 15-24 years, fever and travel were identified as high-risk factors. Malaria was primarily attributed to Plasmodium falciparum mono-infections, followed by P. vivax mono-infections and lastly to falciparum/vivax mixed infections accounting 76.6%, 16.6%, and 6.6% of PCR-confirmed malaria cases, respectively. The nested PCR was superior to the smear microscopy (sensitivity 76.6%; specificity 100%) and the RDT (sensitivity 83.3%, specificity 94.2%). The overall percent agreement between microscopy and the RDT was 92.7% (kappa=0.63). High proportion of imported malaria including sub-microscopic and sub-patent cases were described. We suggest that incorporation of molecular tool into the conventional malaria diagnosis is beneficial in Jazan district.

Citations

Citations to this article as recorded by

Genetic diversity and haplotype structure of the CelTOS gene in Plasmodium falciparum from Jazan Province, Saudi Arabia: implications for vaccine development and malaria elimination
Hani Alothaid, Fatimah Alghnnam, Arwa A. Al-Qahtani, Abdullah Al Marzan, Mashael Abu Alola, Marie Fe F. Bohol, Fatimah Alhamlan, Mohammed I. Shafeai, Fuad H. Rudiny, Ali M. Motaen, Ahmed A. Al-Qahtani
Frontiers in Cellular and Infection Microbiology.2026;[Epub] CrossRef
A study on the prevalence of the malaria parasite in the city of Sebha among expatriate workers from neighboring countries.
Naima I. Al-Haddad , Rugaia M. Elsalem
Libyan Journal of Science &Technology.2025; 15(1): 186. CrossRef
Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy
Ahmed A. Muyidi, Musa A. Ayashi, Majed H. Wakid, Maimonah S. Alghanmi, Fadi M. Baakdah, Hattan S. Gattan, Isra M. Alsaady, Muslimah N. Alsulami, Haleema H. Albohiri, Sarah A. Altwaim, Zaki M. Eisa, Thamer M. Brek, José F. Silveira
Journal of Parasitology Research.2025;[Epub] CrossRef
Asymptomatic/submicroscopic Plasmodium vivax infection: A systematic review and META-analysis on the hidden challenge for preventing re-establishment of malaria transmission
Siqi Wang, He Yan, Li Zhang, Zhigui Xia, Jianhai Yin
Parasite Epidemiology and Control.2025; 30: e00442. CrossRef
The burden, clinical outcomes and risk factors related to neglected tropical diseases and malaria in migrant populations in the Middle East and North Africa: a systematic review and meta-analyses
Eman Elafef, Taha Maatoug, Stella Evangelidou, Helena Marti-Soler, Asad Adam, Ahmed Hamed Arisha, Mahmoud Hilali, Sally Hargreaves, Ibrahim Bani, Farah Seedat, Ana Requena-Méndez
BMJ Global Health.2025; 10(11): e019713. CrossRef
Literature Review on Malaria in Saudi Arabia
Hayel Mohammed Qudsi, Shaza Karim Almowlad, Soha Abdulgader Algamdi, Mohammed Mohammed Bakhiet, Saeed Mahdi Algarni, Emad Lafi Almohammadi, Ali Hassan Alqahtani
Advances in Infectious Diseases.2025; 15(02): 229. CrossRef
Spatiotemporal Distribution of Malaria in the Kingdom of Saudi Arabia
Ahmed Elagali, Mosa Shubayr, Elsiddig Noureldin, Kefyalew Addis Alene, Asmaa Elagali
Tropical Medicine and Infectious Disease.2024; 9(1): 16. CrossRef
Detection of Duffy blood group genotypes and submicroscopic Plasmodium infections using molecular diagnostic assays in febrile malaria patients
Beka R. Abagero, Rei Rama, Abdulghani Obeid, Tirusew Tolosa, Biniyam Lukas, Taye Teka, Daniel Tesfaye, Eugenia Lo, Delenasaw Yewhalaw
Malaria Journal.2024;[Epub] CrossRef
Status of Malaria Infection in Saudi Arabia
Amani Alhejely, V. Trukhachev, A. Skuratov, A. Shitikova, R. Migunov, R.Z. Abbas
BIO Web of Conferences.2024; 139: 06036. CrossRef
Machine learning models for predicting residual malaria infections using environmental factors: A case study of the Jazan region, Kingdom of Saudi Arabia
Idris Zubairu Sadiq, Yakubu Saddeeq Abubakar, Abdulkadir Rabiu Salisu, Babangida Sanusi Katsayal, Umar Saidu, Sani I. Abba, Abdullahi Garba Usman
Decoding Infection and Transmission.2024; 2: 100022. CrossRef
Investigation of Wright Blood Group Alleles and Genotypes in Malaria-Endemic Area in Southwestern Saudi Arabia
Amr Halawani, Saif Abdalla, Mahmoud Habibullah, Ghalia Shamlan, Neil Avent
International Journal of General Medicine.2024; Volume 17: 5175. CrossRef
Knowledge, attitudes, and practices (KAP) during the malaria elimination phase: A household-based cross-sectional survey
Siddig Ibrahim Abdelwahab, Ibrahim M. Elhassan, Osama Albasheer, Manal Mohamed Elhassan Taha, Nasir Ahmed Ali, Yahya Salem Al-Jabiri, Waleed Madkhali, Ahmad A. Sahly, Bassem Oraibi, Ahmed Abdallah Ahmed Altraifi, Nasser Hakami, Mohammed M. Alshehri, Moham
Medicine.2023; 102(22): e33793. CrossRef
Association between interleukin-27 gene polymorphisms and Plasmodium falciparum Malaria
Nada H. Aljarba, Mashael R. Al-Anazi, Tahani M. Al-Hazani, Mohammed I. Shafeai, Fuad H. Rudiny, Ali M. Motaen, Saad M. Bin Dajem, Hani Alothaid, Jahad Alghamdi, Saad Alkahtani, Ahmed A. Al-Qahtani
Innate Immunity.2023; 29(5): 83. CrossRef
Prevalence and proportion estimate of asymptomatic Plasmodium infection in Asia: a systematic review and meta-analysis
Manas Kotepui, Kwuntida Uthaisar Kotepui, Frederick Ramirez Masangkay, Aongart Mahittikorn, Polrat Wilairatana
Scientific Reports.2023;[Epub] CrossRef
A decade of travel-associated malaria at King Fahad Hospital of the University in the Eastern Province of Saudi Arabia
Ashwaq Alanazi, Bayan Ahmad Hasan Almusailhi, Gheed K. Bamousa, Nabaa H. Alhawashim, Nourah M. Alotaibi, Sumiyah AlShamekh, Basavaraja Channabasappa Hunasemarada, Reem Y. Al Jindan, Ayman A. El-Badry
Scientific Reports.2022;[Epub] CrossRef
Malarial Epidemiology among Patients in Al-Medina Al-Munawara, KSA
Shady Mohammad Raji Al-Hussayni , Sami Oudah Eid Al-Harbi , Omar Hassan Amer
International Journal of Life Science and Pharma Research.2022;[Epub] CrossRef
Comparison of Rapid Diagnostic Test, Microscopy, and Polymerase Chain Reaction for the Detection of Plasmodium falciparum Malaria in a Low-Transmission Area, Jazan Region, Southwestern Saudi Arabia
Aymen M. Madkhali, Ahmad Hassn Ghzwani, Hesham M. Al-Mekhlafi
Diagnostics.2022; 12(6): 1485. CrossRef
Genetic Diversity and Population Genetic Analysis of Plasmodium falciparum Thrombospondin Related Anonymous Protein (TRAP) in Clinical Samples from Saudi Arabia
Saad M. Bin Dajem, Md Atique Ahmed, Fatimah F. Alghnnam, Shouq F. Alghannam, Gauspasha Yusuf Deshmukh, Rehan Haider Zaidi, Marie Fe F. Bohol, Syeda Sabiha Salam, Syeda Wasfeea Wazid, Mohammed I. Shafeai, Fuad H. Rudiny, Ali M. Motaen, Kareem Morsy, Ahmed
Genes.2022; 13(7): 1149. CrossRef
Low density Plasmodium infections and G6PD deficiency among malaria suspected febrile individuals in Ethiopia
Beka R. Abagero, Daniel Kepple, Kareen Pestana, Logan Witherspoon, Abdissa Biruksew, Abinet Adanew, Fetiya Baharu, Shantoy Hansel, Karen Lopez, Daniel A. Janies, Eugenia Lo, Delenasaw Yewhalaw
Frontiers in Tropical Diseases.2022;[Epub] CrossRef
Current Status and the Epidemiology of Malaria in the Middle East Region and Beyond
Mohammad Al-Awadhi, Suhail Ahmad, Jamshaid Iqbal
Microorganisms.2021; 9(2): 338. CrossRef
Malaria Rapid Diagnostic Tests: Literary Review and Recommendation for a Quality Assurance, Quality Control Algorithm
Michael J. Kavanaugh, Steven E. Azzam, David M. Rockabrand
Diagnostics.2021; 11(5): 768. CrossRef
Current Epidemiological Characteristics of Imported Malaria, Vector Control Status and Malaria Elimination Prospects in the Gulf Cooperation Council (GCC) Countries
Jamshaid Iqbal, Suhail Ahmad, Ali Sher, Mohammad Al-Awadhi
Microorganisms.2021; 9(7): 1431. CrossRef
Residual malaria in Jazan region, southwestern Saudi Arabia: the situation, challenges and climatic drivers of autochthonous malaria
Hesham M. Al-Mekhlafi, Aymen M. Madkhali, Khalid Y. Ghailan, Ahmed A. Abdulhaq, Ahmad Hassn Ghzwani, Khalid Ammash Zain, Wahib M. Atroosh, Alkhansa Alshabi, Hussein A. Khadashi, Majid A. Darraj, Zaki M. Eisa
Malaria Journal.2021;[Epub] CrossRef
Case Report: Case report: Mixed infection of Plasmodium vivax and Plasmodium falciparum in a tertiary hospital
Abeer M. Al-Subaie
F1000Research.2021; 10: 779. CrossRef
The global burden of Plasmodium vivax malaria is obscure and insidious
Katherine E. Battle, J. Kevin Baird, Lorenz von Seidlein
PLOS Medicine.2021; 18(10): e1003799. CrossRef
The Distribution of Malaria with Seasonal in Kokap Yogyakarta 2012-2017
Raden A.K. Lestari, Novyan Lusiyana, Fitria S. Nurochmah, G. Gholib, A. Sutriana, A. Engelhardt, J. Duboscq, R. Sahara Zamzami
E3S Web of Conferences.2020; 151: 01018. CrossRef
Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia
Yiu‐Wing Kam, Mohamed Yousif Ahmed, Siti Naqiah Amrun, Bernett Lee, Tarik Refaie, Kamla Elgizouli, Siew‐Wai Fong, Laurent Renia, Lisa FP Ng
Clinical & Translational Immunology.2020;[Epub] CrossRef
Rapid diagnostic tests for Plasmodium vivax malaria in endemic countries
Ridhi Agarwal, Leslie Choi, Samuel Johnson, Yemisi Takwoingi
Cochrane Database of Systematic Reviews.2020;[Epub] CrossRef
Decreasing trend of imported malaria cases but increasing influx of mixed P. falciparum and P. vivax infections in malaria-free Kuwait
Jamshaid Iqbal, Mohammad Al-Awadhi, Suhail Ahmad, Luzia Helena Carvalho
PLOS ONE.2020; 15(12): e0243617. CrossRef
Preparedness against self-infection and importation of Malaria - An airport survey among Saudis traveling to endemic countries
HaninSaleh AlOwaini, RizwanSuliankatchi Abdulkader, QusayFahad Almahmoud, AbdulazizEyad Alqudaimi, RaneemSaied Al-Ghamdi, WateenAhmed Alhamoud, Hasan Keriri
Journal of Family Medicine and Primary Care.2019; 8(10): 3318. CrossRef

11,150 View
171 Download
24 Web of Science
Crossref

Brief Communication

Comparison of Seropositivity to Trichomonas vaginalis between Men with Prostatic Tumor and Normal Men: Jung-Hyun Kim, Hong-Sang Moon, Kyu-Shik Kim, Hwan-Sik Hwang, Jae-Sook Ryu, Sung-Yul Park; Korean J Parasitol 2019;57(1):21-25.
Published online February 26, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.1.21

Abstract
PDF

Trichomoniasis is the most common curable sexually-transmitted infection. Most Trichomonas vaginalis-infected men are asymptomatic and can remain undiagnosed and untreated, and this has been thought to result in chronic persistent prostatic infection. Chronic inflammation is regarded as the major factor in the pathogenesis and progression of benign prostatic hyperplasia (BPH) and prostatic cancer (PCa). The aim of this study is to identify seropositivity to T. vaginalis in men with prostate tumors (BPH or PCa) visited to Hanyang University Hospital. A total of 183 men were enrolled between October 2013 and November 2014. They consisted of 139 with BPH (mean age: 64.0 ± 0.07) and 44 with prostate cancer (mean age: 73.3±0.18). We carried out ELISA to identify the seropositivity to T. vaginalis. Mixed lysate antigen extracted from 8 strains of T. vaginalis was used in the ELISA. Also 58 male outpatients visited to Health Promotion Center in Hanyang University Hospital were evaluated for comparing group. As a results, seropositivity to T. vaginalis in patients with prostatic diseases was 19.7% (BPH: 18.7%, PCa: 22.7%) and it was significantly higher than the 1.7% of the comparing healthy group (P = 0.001). Therefore, prostatic tumor showed higher seropositivity against T. vaginalis than normal men. As far as we know, this is the first report about seroprevalence in prostatic tumor in Korea.

Citations

Citations to this article as recorded by

Alloferon regulates the growth and movement of Trichomonas vaginalis by altering hydrogenosomes
Hyejung Jo, Minsoo Kang, Yejin Kim, Jae Seung Kang
BMC Infectious Diseases.2026;[Epub] CrossRef
The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis
Alexandra Ibáñez-Escribano, Juan José Nogal-Ruiz
Pathogens.2024; 13(2): 126. CrossRef
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China
Zhenchao Zhang, Yuhui Sang, Pucheng Wu, Yujia Shang, Lesong Li, Yujuan Duan, Linfei Zhao, Minghui Gao, Lihua Guo, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lixia Hao, Xuefang Mei, Jianbing Mu
Journal of Tropical Medicine.2023; 2023: 1. CrossRef
Inflammatory responses during trichomoniasis: The role of Toll‐like receptors and inflammasomes
Abdollah Jafarzadeh, Maryam Nemati, Ehsan Salarkia, Sonal Yadav, Najmeh Aminizadeh, Sara Jafarzadeh, Manisha Yadav
Parasite Immunology.2023;[Epub] CrossRef
No Association of Trichomonas vaginalis Seropositivity with Advanced Prostate Cancer Risk in the Multiethnic Cohort: A Nested Case-Control Study
Michelle Nagata, Anne Tome, Kami White, Lynne R. Wilkens, Song-Yi Park, Loïc Le Marchand, Christopher Haiman, Brenda Y. Hernandez
Cancers.2023; 15(21): 5194. CrossRef
Identification of Signature Genes and Construction of an Artificial Neural Network Model of Prostate Cancer
Hongye Dong, Xu Wang, Kalidoss Rajakani
Journal of Healthcare Engineering.2022; 2022: 1. CrossRef
Multiple pathogens and prostate cancer
James S. Lawson, Wendy K. Glenn
Infectious Agents and Cancer.2022;[Epub] CrossRef
Trichomonas vaginalis serostatus and prostate cancer risk in Egypt: a case-control study
Nora E. Saleh, Samar M. Alhusseiny, Wafaa M. El-Zayady, Engy M. Aboelnaga, Wafaa N. El-beshbishi, Yasser M. Saleh, Hala S. Abou-ElWafa, Samar N. El-Beshbishi
Parasitology Research.2021; 120(4): 1379. CrossRef
The Role of Microbial Factors in Prostate Cancer Development—An Up-to-Date Review
Karolina Garbas, Piotr Zapała, Łukasz Zapała, Piotr Radziszewski
Journal of Clinical Medicine.2021; 10(20): 4772. CrossRef
Involvement of Macrophages in Proliferation of Prostate Cancer Cells Infected with Trichomonas vaginalis
Kyu-Shik Kim, Hong-Sang Moon, Sang-Su Kim, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(6): 557. CrossRef
Seroprevalence and risk factors of Trichomonas vaginalis among couples in Al-Hamza city-Iraq.
Musafer H. Al-Ardi
Al-Kufa University Journal for Biology.2021; 13(1): 26. CrossRef
Is Trichomonas vaginalis a Risk Factor for Prostate Cancer? A Systematic Review and Meta-analysis
Gianpaolo Perletti, Vittorio Magri, Louise Beckers-Perletti, Alberto Trinchieri, Konstantinos Stamatiou
Hellenic Urology.2021; 33(1): 12. CrossRef
IL-6 produced by prostate epithelial cells stimulated with Trichomonas vaginalis promotes proliferation of prostate cancer cells by inducing M2 polarization of THP-1-derived macrophages
Ik-Hwan Han, Hyun-Ouk Song, Jae-Sook Ryu, Michael H. Hsieh
PLOS Neglected Tropical Diseases.2020; 14(3): e0008126. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang
BMC Infectious Diseases.2020;[Epub] CrossRef
The Crosstalk between Prostate Cancer and Microbiota Inflammation: Nutraceutical Products Are Useful to Balance This Interplay?
Felice Crocetto, Mariarosaria Boccellino, Biagio Barone, Erika Di Zazzo, Antonella Sciarra, Giovanni Galasso, Giuliana Settembre, Lucio Quagliuolo, Ciro Imbimbo, Silvia Boffo, Italo Francesco Angelillo, Marina Di Domenico
Nutrients.2020; 12(9): 2648. CrossRef

10,114 View
152 Download
13 Web of Science
Crossref

Original Articles

Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus: Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Chom-Kyu Chong, Yanaihara Pinchemel, Francis Reisdo?rfer, Joyce Brito Coelho, Ronaldo Ferreira Dias, Pan Kee Bae, Zuinara Pereira Maia Gusma?o, Hye-Jin Ahn, Ho-Woo Nam; Korean J Parasitol 2018;56(1):61-70.
Published online February 28, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.1.61

Abstract
PDF

We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV. These proteins were produced using baculovirus expression vector with Sf9 cells. Monoclonal antibodies J2G7 to NS1 and J5E1 to E protein were selected and conjugated with colloidal gold to produce the Zika IgG/IgM RDT kit (Zika RDT). Comparisons with ELISA, plaque reduction neutralization test (PRNT), and PCR were done to investigate the analytical sensitivity of Zika RDT, which resulted in 100% identical results. Sensitivity and specificity of Zika RDT in a field test was determined using positive and negative samples from Brazil and Korea. The diagnostic accuracy of Zika RDT was fairly high; sensitivity and specificity for IgG was 99.0 and 99.3%, respectively, while for IgM it was 96.7 and 98.7%, respectively. Cross reaction with dengue virus was evaluated using anti-Dengue Mixed Titer Performance Panel (PVD201), in which the Zika RDT showed cross-reactions with DENV in 16.7% and 5.6% in IgG and IgM, respectively. Cross reactions were not observed with West Nile, yellow fever, and hepatitis C virus infected sera. Zika RDT kit is very simple to use, rapid to assay, and very sensitive, and highly specific. Therefore, it would serve as a choice of method for point-of-care diagnosis and large scale surveys of ZIKV infection under clinical or field conditions worldwide in endemic areas.

Citations

Citations to this article as recorded by

The expanding toolkit of insect cell culture: a new era in biotechnology
Surjeet Kumar Arya, Cynthia L Goodman, Subba Reddy Palli
Current Opinion in Insect Science.2026; 74: 101465. CrossRef
Advancing point-of-care diagnostics: Engineering and enhancing sensitivity and specificity in nanoparticle-based lateral flow assays for rapid disease detection
Arunima Lala, Thangapandi Kalyani, Hiranmoy Kotal, Saikat Kumar Jana
Talanta.2026; 303: 129450. CrossRef
Human scFv antibodies selected by phage display technology exhibit distinct Zika virus binding profiles and minimal cross-reactivity with dengue virus serotype 2
Hernan H.M. da Costa, Isaura B.B. Silva, Renato K.A.O. França, Carlos H.R. Gomes, Luciana M. Sérgio, Marcelo M. Brígido, Andréa Q. Maranhão, Ricardo J.S. Torquato, Elizabeth de Gaspari, Ramendra P. Pandey, Denise C.A. Oliveira, Viviane F. Botosso, Renato
International Journal of Biological Macromolecules.2026; 363: 152125. CrossRef
Development of a colloidal gold immunochromatographic strip to detect equine infectious anemia virus
Jianzhong Wang, Jicheng Qiu, Mengmeng Wang, Xiaojie Wu, Xiaoguang Li, Heng Zhang
Virology Journal.2025;[Epub] CrossRef
Advances in Metallic-Based Localized Surface Plasmon Sensors for Enhanced Tropical Disease Detection: A Comprehensive Review
Sajid Farooq, Denise Maria Zezell
Plasmonics.2024; 19(4): 1721. CrossRef
Diagnostic accuracy of DPP Fever Panel II Asia tests for tropical fever diagnosis
Sandhya Dhawan, Sabine Dittrich, Sonia Arafah, Stefano Ongarello, Aurelian Mace, Siribun Panapruksachat, Latsaniphone Boutthasavong, Aphaphone Adsamouth, Soulignasak Thongpaseuth, Viengmon Davong, Manivanh Vongsouvath, Elizabeth A. Ashley, Matthew T. Robi
PLOS Neglected Tropical Diseases.2024; 18(4): e0012077. CrossRef
Hyperendemic Dengue and Possible Zika Circulation in the Westernmost Region of the Indonesian Archipelago
Harapan Harapan, Kritu Panta, Alice Michie, Timo Ernst, Suzi McCarthy, Muhsin Muhsin, Safarianti Safarianti, Tjut Mariam Zanaria, Mudatsir Mudatsir, R. Tedjo Sasmono, Allison Imrie
Viruses.2022; 14(2): 219. CrossRef
Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology
Thai Leong Yap, Shin Yee Hong, Jun Hui Soh, Lekha Ravichandraprabhu, Vanessa W.X. Lim, Hsi-Min Chan, Tommy Z.X. Ong, Ying Ping Chua, Shi En Koh, Huajing Wang, Yee Sin Leo, Jackie Y. Ying, William Sun
Emerging Infectious Diseases.2021; 27(5): 1427. CrossRef
Development and characterization of mouse monoclonal antibodies targeting to distinct epitopes of Zika virus envelope protein for specific detection of Zika virus
Chia-Jung Li, Ping-Han Huang, Hui-Wen Chen, Shih-Chung Chang
Applied Microbiology and Biotechnology.2021; 105(11): 4663. CrossRef
Recent advances in point-of-care biosensors for the diagnosis of neglected tropical diseases
Patricia Batista Deroco, Dagwin Wachholz Junior, Lauro Tatsuo Kubota
Sensors and Actuators B: Chemical.2021; 349: 130821. CrossRef
Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies
Alexandra Marisa Targovnik, Jorge Alejandro Simonin, Gregorio Juan Mc Callum, Ignacio Smith, Franco Uriel Cuccovia Warlet, María Victoria Nugnes, María Victoria Miranda, Mariano Nicolás Belaich
Applied Microbiology and Biotechnology.2021; 105(21-22): 8195. CrossRef
Strategies for developing sensitive and specific nanoparticle-based lateral flow assays as point-of-care diagnostic device
Jun Hui Soh, Hsi-Min Chan, Jackie Y. Ying
Nano Today.2020; 30: 100831. CrossRef
Evolutions and upcoming on Zika virus diagnosis through an outbreak: A systematic review
Fernando A. Jorge, Mateus V. Thomazella, Deborah de Castro Moreira, Luciana D. G. Lopes, Jorge J. V. Teixeira, Dennis A. Bertolini
Reviews in Medical Virology.2020;[Epub] CrossRef
Zika virus serological diagnosis: commercial tests and monoclonal antibodies as tools
Isaura Beatriz Borges Silva, Aldacilene Souza da Silva, Mariana Sequetin Cunha, Aline Diniz Cabral, Kelly Cristina Alves de Oliveira, Elizabeth De Gaspari, Carlos Roberto Prudencio
Journal of Venomous Animals and Toxins including Tropical Diseases.2020;[Epub] CrossRef
ZIKV-Specific NS1 Epitopes as Serological Markers of Acute Zika Virus Infection
Yiu-Wing Kam, Juliana Almeida Leite, Siti Naqiah Amrun, Fok-Moon Lum, Wearn-Xin Yee, Farhana Abu Bakar, Kai Er Eng, David C Lye, Yee-Sin Leo, Chia-Yin Chong, Andre Ricardo Ribas Freitas, Guilherme Paier Milanez, Jose Luiz Proença-Modena, Laurent Rénia, Fa
The Journal of Infectious Diseases.2019; 220(2): 203. CrossRef
Seasonal dengue surge: Providers⬨tm) perceptions about the impact of dengue on patient volume, staffing and use of point of care testing in Indian emergency departments
Janice Blanchard, Katherine Douglass, Shweta Gidwani, Usha Khatri, Daniel Gaballa, Amelia Pousson, Neeraj Mangla, Jeffrey Smith
Journal of Infection and Public Health.2019; 12(6): 794. CrossRef
Development and Clinical Evaluation of a Rapid Diagnostic Test for Yellow Fever Non-Structural Protein 1
Yeong Hoon Kim, Tae-Yun Kim, Ji-Seon Park, Jin Suk Park, Jihoo Lee, Joungdae Moon, Chom-Kyu Chong, Ivan Neves Junior, Fernando Raphael Ferry, Hye-Jin Ahn, Lokraj Bhatt, Ho-Woo Nam
The Korean Journal of Parasitology.2019; 57(3): 283. CrossRef
Zika Fever: Development of Diagnostics, Prevention and Treatment
E. I. Kazachinskaya, D. V. Shan’shin, A. V. Ivanova
Problems of Particularly Dangerous Infections.2019; (2): 6. CrossRef
High correlation between Zika virus NS1 antibodies and neutralizing antibodies in selected serum samples from normal healthy Thais
Wannapa Sornjai, Suwipa Ramphan, Nitwara Wikan, Prasert Auewarakul, Duncan R. Smith
Scientific Reports.2019;[Epub] CrossRef
Generation and Characterization of a Polyclonal Antibody Against NS1 Protein for Detection of Zika Virus
Liding Zhang, Congjie Chen, Zhixin Chen, Shuzhen He, Yuzhu Song, Xueshan Xia, Qinqin Han, Jinyang Zhang
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact With trVLP Elements
Dong-Shan Yu, Tian-Hao Weng, Chen-Yu Hu, Zhi-Gang Wu, Yan-Hua Li, Lin-Fang Cheng, Nan-Ping Wu, Lan-Juan Li, Hang-Ping Yao
Frontiers in Microbiology.2018;[Epub] CrossRef
Analysis of Zika virus neutralizing antibodies in normal healthy Thais
Wannapa Sornjai, Janejira Jaratsittisin, Prasert Auewarakul, Nitwara Wikan, Duncan R. Smith
Scientific Reports.2018;[Epub] CrossRef

16,228 View
417 Download
22 Web of Science
Crossref

Population Genetics of Plasmodium vivax in Four High Malaria Endemic Areas in Thailand: Kanungnit Congpuong, Ratawan Ubalee; Korean J Parasitol 2017;55(5):465-472.
Published online October 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.5.465

Abstract
PDF

Recent trends of malaria in Thailand illustrate an increasing proportion of Plasmodium vivax, indicating the importance of P. vivax as a major causative agent of malaria. P. vivax malaria is usually considered a benign disease so the knowledge of this parasite has been limited, especially the genetic diversity and genetic structure of isolates from different endemic areas. The aim of this study was to examine the population genetics and structure of P. vivax isolates from 4 provinces with different malaria endemic settings in Thailand using 6 microsatellite markers. Total 234 blood samples from P. vivax mono-infected patients were collected. Strong genetic diversity was observed across all study sites; the expected heterozygosity values ranged from 0.5871 to 0.9033. Genetic variability in this study divided P. vivax population into 3 clusters; first was P. vivax isolates from Mae Hong Son and Kanchanaburi Provinces located on the western part of Thailand; second, Yala isolates from the south; and third, Chanthaburi isolates from the east. P. vivax isolates from patients having parasite clearance time (PCT) longer than 24 hr after the first dose of chloroquine treatment had higher diversity when compared with those having PCT within 24 hr. This study revealed a clear evidence of different population structure of P. vivax from different malaria endemic areas of Thailand. The findings provide beneficial information to malaria control programme as it is a useful tool to track the source of infections and current malaria control efforts.

Citations

Citations to this article as recorded by

Low Genetic Diversity of Plasmodium vivax Circumsporozoite Surface Protein in Clinical Isolates from Southern Thailand
Tachin Khulmanee, Thanyapit Thita, Kanyanan Kritsiriwutinan, Usa Boonyuen, Aminoh Saai, Kanjana Inkabjan, Rimi Chakrabarti, Pradipsinh K. Rathod, Srivicha Krudsood, Mathirut Mungthin, Rapatbhorn Patrapuvich
Tropical Medicine and Infectious Disease.2024; 9(5): 94. CrossRef
Plasmodium vivax populations in the western Greater Mekong Subregion evaluated using a genetic barcode
Yubing Hu, Yuling Li, Awtum M. Brashear, Weilin Zeng, Zifang Wu, Lin Wang, Haichao Wei, Myat Thu Soe, Pyae Linn Aung, Jetsumon Sattabongkot, Myat Phone Kyaw, Zhaoqing Yang, Yan Zhao, Liwang Cui, Yaming Cao, Karin Kirchgatter
PLOS Neglected Tropical Diseases.2024; 18(7): e0012299. CrossRef
Extensive genetic diversity in Plasmodium vivax from Sudan and its genetic relationships with other geographical isolates
Musab M. Ali Albsheer, Alfred Hubbard, Cheikh Cambel Dieng, Eyoab Iyasu Gebremeskel, Safaa Ahmed, Virginie Rougeron, Muntaser E. Ibrahim, Eugenia Lo, Muzamil M. Abdel Hamid
Infection, Genetics and Evolution.2024; 123: 105643. CrossRef
Comparison of Vector Trapping Methods for Outdoor Biting Malaria Vector Surveillance in Thailand and Vietnam
Ratchadawan Ngoenklan, Tran Thanh Duong, Vu Duc Chinh, Nguyen Quang Thieu, Jeffrey Hii, Michael J Bangs, Boonserm Aum-Aung, Wannapa Suwonkerd, Theeraphap Chareonviriyaphap, Nobuko Tuno
Journal of Medical Entomology.2022; 59(6): 2139. CrossRef
Dynamics of Plasmodium vivax populations in border areas of the Greater Mekong sub-region during malaria elimination
Yuling Li, Yubing Hu, Yan Zhao, Qinghui Wang, Huguette Gaelle Ngassa Mbenda, Veerayuth Kittichai, Saranath Lawpoolsri, Jetsumon Sattabongkot, Lynette Menezes, Xiaoming Liu, Liwang Cui, Yaming Cao
Malaria Journal.2020;[Epub] CrossRef

9,384 View
230 Download
5 Web of Science
Crossref

Case Reports

A Case of Pentastomiasis at the Left Maxilla Bone in a Patient with Thyroid Cancer: Eunae Sandra Cho, Seung Wook Jung, Hwi-Dong Jung, In Yong Lee, Tai-Soon Yong, Su Jin Jeong, Hyun Sil Kim; Korean J Parasitol 2017;55(4):433-437.
Published online August 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.4.433

Abstract
PDF

Pentastomiasis, a zoonotic parasite infection, is typically found in the respiratory tract and viscera of the host, including humans. Here, we report for the first time an extremely rare case of intraosseous pentastomiasis in the human maxilla suffering from medication related osteonecrosis of the jaw (MRONJ). A 55-year-old male had continuously visited the hospital for MRONJ which had primarily developed after bisphosphonate and anti-neoplastic administration for previous bone metastasis of medullary thyroid cancer. Pain, bone exposure, and pus discharge in the right mandible and left maxilla were seen. Osteolysis with maxillary cortical bone perforation at the left buccal vestibule, palate, nasal cavity, and maxillary sinus was observed by radiologic images. A biopsy was done at the left maxilla and through pathological evaluation, a parasite with features of pentastome was revealed within the necrotic bone tissue. Further history taking and laboratory evaluation was done. The parasite was suspected to be infected through maxillary open wounds caused by MRONJ. Awareness of intraosseous pentastomiasis should be emphasized not to be missed behind the MRONJ. Proper evaluation and interpretation for past medical history may lead to correct differential diagnosis and therapeutic intervention for parasite infections.

Citations

Citations to this article as recorded by

Medication-Related Osteonecrosis of the Jaw: A Systematic Review of Case Reports and Case Series
Filipa Frutuoso, Filipe Freitas, Miguel Vilares, Helena Francisco, Duarte Marques, João Caramês, André Moreira
Diseases.2024; 12(9): 205. CrossRef
Cancerogenic parasites in veterinary medicine: a narrative literature review
Niccolò Fonti, Francesca Parisi, Francesca Mancianti, Giulia Freer, Alessandro Poli
Infectious Agents and Cancer.2023;[Epub] CrossRef
Morphological and molecular description of Armillifer moniliformis larvae isolated from Sri Lankan brown palm civet (Paradoxurus montanus)
C. Rajapaksha, A. P. Amarasinghe, S. Fernando, R. P. V. J. Rajapakse, D. Tappe, S. Wickramasinghe
Parasitology Research.2020; 119(3): 773. CrossRef
Answer to January 2021 Photo Quiz
Damien Costa, Pascal Rottenberg, Emmanuel Toure, Pascal Delaunay, Loic Favennec, Gilles Gargala, Erik Munson
Journal of Clinical Microbiology.2020;[Epub] CrossRef
Identifikasi Parasit Pentastomida pada Biawak Air (Varanus salvator) yang akan dikonsumsi
Andhika Yudhantama Subroto, I Komang Wiarsa Sardjana, Moh Sukmanadi, E Djoko Poetranto, Kusnoto Kusnoto, Agus Sunarso
Journal of Parasite Science.2020;[Epub] CrossRef
Capecitabine/everolimus/zoledronic acid

Reactions Weekly.2017; 1676(1): 76. CrossRef

13,257 View
158 Download
5 Web of Science
Crossref

Unusual Location of Hydatid Cysts: Report of Two Cases in the Heart and Hip Joint of Romanian Patients: Simona Gurzu, Marius Alexandru Beleaua, Emeric Egyed-Zsigmond, Ioan Jung; Korean J Parasitol 2017;55(4):429-431.
Published online August 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.4.429

Abstract
PDF

Hydatid cyst is usually located in the liver and lungs, rare cases showing localization in other organs or tissues. In the unusual location, echinococcosis is an excluding diagnosis that is established only after microscopic evaluation. Our first case occurred in a 67-year-old female previously diagnosed with pulmonary tuberculosis and hospitalized with persistent pain in the hip joint. The clinical diagnosis was tuberculosis of the joint, but the presence of the specific acellular membrane indicated a hydatid cyst of the synovial membrane, without bone involvement. Fewer than 25 cases of joint hydatidosis have been reported in literature to date. In the second case, the intramural hydatid cyst was incidentally discovered at autopsy, in the left heart ventricle of a 52-year-old male hospitalized for a fatal brain hemorrhage, as a result of rupture of an anterior communicating artery aneurysm. The conclusion of our paper is that echinococcosis should be taken into account for the differential diagnosis of cystic lesions, independently from their location.

Citations

Citations to this article as recorded by

Unusual primary hydatid cysts of the body without liver and lung involvement
Yener Aydin, Ali Bilal Ulas, Suat Eren, Gurkan Ozturk, Kamber Kasali, Yilmaz Aksoy, Mehmet Kursat Karadag, Eyup Senocak, Abdurrahim Colak
Journal of Parasitic Diseases.2025;[Epub] CrossRef
Cardiac cystic echinococcosis—A systematic review and analysis of the literature
Simone Bumann, Esther Kuenzli, Raffaella Lissandrin, Enrico Brunetti, Sam Goblirsch, Lars Henning, Francesca Tamarozzi, Andreas Neumayr, Adriano Casulli
PLOS Neglected Tropical Diseases.2024; 18(5): e0012183. CrossRef
Cardiac hydatid disease with palpitations as the only presenting complaint: the egg in the nest
Ali Azari, Leila Bigdelu, Ossama Maadarani, Zouheir Bitar
European Journal of Case Reports in Internal Medicine.2023;[Epub] CrossRef
Renal hydatid cyst mimicked cystic renal cell carcinoma: A case report
Xiyi Wei, Jinyong Tian, Jianyu Diao, Gulinuer Aibibula, Maimaitijiang Dawuti, Yiliyasi Tuerxun, Mhtaer Wubuli, Yujie Zhang, Ninghong Song, Jie Yang
Precision Medical Sciences.2022; 11(1): 35. CrossRef
Cardiac hydatid cysts in a young man: A case report and a literature review
Mircea Bajdechi, Dalia Manolache, Adrian Tudor, Mihnea Orghidan, Adriana Gurghean
Experimental and Therapeutic Medicine.2022;[Epub] CrossRef
Cystic echinococcosis: a 10-year experience from a middle-income country
Ayesha Butt, Javaid Ahmed Khan
Tropical Doctor.2020; 50(2): 117. CrossRef
Uncommon Locations of Cystic Echinococcosis: A Report of 46 Cases from Southern Iran
Reza Shahriarirad, Amirhossein Erfani, Mehrdad Eskandarisani, Mohammad Rastegarian, Bahador Sarkari
Surgery Research and Practice.2020; 2020: 1. CrossRef
Alveolar echinococcosis in the head of pancreas
Rong-Xing Zhou, Hai-Jie Hu, Wen-Jie Ma, Yong Jiang, Fu-Yu Li
Medicine.2018; 97(11): e0072. CrossRef

8,054 View
171 Download
7 Web of Science
Crossref

Original Article

Hookworm Infection: A Neglected Cause of Overt Obscure Gastrointestinal Bleeding: Kun-Yan Wei, Qiong Yan, Bo Tang, Shi-Ming Yang, Peng-Bing Zhang, Ming-Ming Deng, Mu-Han L?; Korean J Parasitol 2017;55(4):391-398.
Published online August 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.4.391

Abstract
PDF

Hookworm infections are widely prevalent in tropical and subtropical areas, especially in low income regions. In the body, hookworms parasitize the proximal small intestine, leading to chronic intestinal hemorrhage and iron deficiency anemia. Occasionally, hookworms can cause overt gastrointestinal bleeding, but this is often ignored in heavily burdened individuals from endemic infectious areas. A total of 424 patients with overt obscure gastrointestinal bleeding were diagnosed by numerous blood tests or stool examinations as well as esophagogastroduodenoscopy, colonoscopy, capsule endoscopy or double-balloon enteroscopy. All of the patients lived in hookworm endemic areas and were not screened for hookworm infection using sensitive tests before the final diagnosis. The patients recovered after albendazole treatment, blood transfusion, and iron replacement, and none of the patients experienced recurrent bleeding in the follow-up. All the 31 patients were diagnosed with hookworm infections without other concomitant bleeding lesions, a rate of 7.3% (31/424). Seventeen out of 227 patients were diagnosed with hookworm infections in the capsule endoscopy (CE), and 14 out of 197 patients were diagnosed with hookworm infections in the double balloon enteroscopy (DBE). Hookworm infections can cause overt gastrointestinal bleeding and should be screened in patients with overt obscure gastrointestinal bleeding (OGIB) in endemic infectious areas with sensitive methods. Specifically, the examination of stool specimens is clinically warranted for most patients, and the proper examination for stool eggs relies on staff’s communication.

Citations

Citations to this article as recorded by

Hookworm as an overlooked cause of overt gastrointestinal bleeding: A case report
Dibya Jyoti Sharma, Aritra Banerjee, Dibyalochan Praharaj, Deepak K. Pandey, Suman Sarkar
Tropical Doctor.2026; 56(1): 169. CrossRef
Combined morphological and molecular approaches to the clinical diagnosis of Necator americanus infection: a case report
Xianshu Liu, Ailian Sun, Xingxing Zheng, Meng Xia, Yan Liu, Shuaiqin Huang, Xiang Wu
Journal of Medical Case Reports.2025;[Epub] CrossRef
Hookworm: a challenge diagnosis of occult gastrointestinal bleeding
A C Garcia, M Francisco, H Coelho, S Bragança, F Pereira Correia, M L Figueiredo, G Alexandrino, J C Branco, D Horta, M Alexandra
Endoscopy.2025; 57(S 02): S415. CrossRef
Soil-Transmitted Helminth Infection and Macronutrient Intake among Stunted Toddlers in Panti Sub-district, Jember
Ghaiska Najma Amnur, Yunita Armiyanti, Irawan Fajar Kusuma, Leersia Yusi Ratnawati, Wiwien Sugih Utami, Bagus Hermansyah
Media Gizi Indonesia.2025; 20(1): 74. CrossRef
Hookworm - Associated Iron Deficiency Anemia at Age Extremes: Two Case Reports from North Sulawesi, Indonesia
Diana Purwanto, Josef Tuda
International Journal of Infectious Diseases and Therapy.2025; 10(4): 85. CrossRef
Hookworm infestation as a rare cause of melaena in tropics: A case report
Narendra Pandit, Sameer Bhattarai, Dinesh Nalbo, Sanjay Yadav
Tropical Doctor.2024; 54(2): 191. CrossRef
Chronic Hemorrhagic Anemia Caused by Hookworm Infection: A Case Report
Baisheng Li, Shihong Chen, Xiangming Cui, Ming Dai, Wenying Meng, Qiong Wu, Huiming Sheng
Acta Parasitologica.2023; 68(1): 288. CrossRef
Infecciones parasitarias del intestino
P. Bourée
EMC - Tratado de Medicina.2023; 27(4): 1. CrossRef
Endoscopic Diagnosis of Hookworm Disease in a Patient with Severe Iron Deficiency Anemia: A Case Report
Shamil Tiremo, Mulugeta Shibeshi
International Medical Case Reports Journal.2023; Volume 16: 841. CrossRef
Cumulative host energetic costs of soil-transmitted helminth infection
Theresa E. Gildner, Tara J. Cepon-Robins, Samuel S. Urlacher
Trends in Parasitology.2022; 38(8): 629. CrossRef
Human hookworms from Argentina: Differential diagnosis of Necator americanus and Ancylostoma duodenale in endemic populations from Buenos Aires and Misiones
Andrea Servián, Silvia A. Repetto, María Lorena Zonta, Graciela T. Navone
Revista Argentina de Microbiología.2022; 54(4): 268. CrossRef
Unusual Cause of Persistent Epistaxis with Severe Anemia in a Child
Yilkal Zemene, Tadele Hailu, Josh Wiedermann, Seckin Ulualp
Case Reports in Otolaryngology.2022; 2022: 1. CrossRef
Haematological abnormalities in children with sickle cell disease and non-severe malaria infection in western Kenya
Paul Kosiyo, Walter Otieno, Jesse Gitaka, Elly O. Munde, Collins Ouma
BMC Infectious Diseases.2021;[Epub] CrossRef
Perceptions of women, their husbands and healthcare providers about anemia in rural Pakistan: Findings from a qualitative exploratory study
Sumera Aziz Ali, Anam Feroz, Zahid Abbasi, Savera Aziz Ali, Ahreen Allana, K. Michael Hambidge, Nancy F. Krebs, Jamie E. Westcott, Elizabeth M. McClure, Robert L. Goldenberg, Sarah Saleem, Frank T. Spradley
PLOS ONE.2021; 16(4): e0249360. CrossRef
Evaluation of Hookworm Diagnosis Techniques from Patients in Debre Elias and Sanja Districts of the Amhara Region, Ethiopia
Ayalew Jejaw Zeleke, Ayenew Addisu, Adane Derso, Yalewayker Tegegne, Meseret Birhanie, Tekeba Sisay, Mulugeta Aemero, José F. Silveira
Journal of Parasitology Research.2021; 2021: 1. CrossRef
Identification and localization of hookworm platelet inhibitor in Ancylostoma ceylanicum
Yue Huang, Asmaa M.I. Abuzeid, Yunqiu Liu, Long He, Qi Zhao, Xinxin Yan, Jianxiong Hang, Rongkun Ran, Yongxiang Sun, Xiu Li, Jumei Liu, Guoqing Li
Infection, Genetics and Evolution.2020; 77: 104102. CrossRef
Effect of Ancylostoma ceylanicum hookworm platelet inhibitor on platelet adhesion and peripheral blood mononuclear cell proliferation
Yue Huang, Asmaa M.I. Abuzeid, Tingting Zhuang, Shilan Zhu, Long He, Yunqiu Liu, Qi Zhao, Xiaoyu Chen, Guoqing Li
Parasitology Research.2020; 119(6): 1777. CrossRef
Intestinal hookworm infestation causing chronic anaemia
Marcello Cintolo, Lorenzo Dioscoridi, Mutaz Massad, Massimiliano Mutignani
BMJ Case Reports.2019; 12(1): bcr-2018-228120. CrossRef
Ancylostoma duodenale as a cause of upper gastrointestinal bleeding: a case report
Andrea Carlin Ronquillo, Lidia Benites Puelles, Luis Pampa Espinoza, Víctor Aguilar Sánchez, José Luis Pinto Valdivia
The Brazilian Journal of Infectious Diseases.2019; 23(6): 471. CrossRef

13,426 View
225 Download
18 Web of Science
Crossref

Brief Communication

Artyfechinostomum malayanum: Metacercariae Encysted in Pila sp. Snails Purchased from Phnom Penh, Cambodia: Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Muth Sinuon, Hoo-Gn Jeoung, Jong-Yil Chai; Korean J Parasitol 2017;55(3):341-345.
Published online June 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.3.341

Abstract
PDF

The metacercariae of Artyfechinostomum malayanum (Leiper, 1911) Mendheim, 1943 were discovered in Pila sp. snails purchased from a market in Phnom Penh, Cambodia. They were isolated from the snails using the artificial digestion technique and were orally fed to 2 hamsters, 1 rat, and 2 mice to obtain the adult flukes. The metacercariae were round, 145-165 μm in diameter, having a cyst wall of 6-10 μm in thickness, a head collar and collar spines, and characteristic features of excretory granules. Adult flukes were recovered in the small intestines of the animals at days 14 and 32 post infection and were morphologically observed using a light microscope and a scanning electron microscope. They were plump or elongated, ventrally curved, 6.0-8.1×1.6-2.0 mm in size, and characterized by the head collar bearing 43 collar spines, including 5 end group ones on each side, a long cirrus sac extending beyond the posterior margin of the ventral sucker, a submedian ovary, and 2 deeply lobed testes. Eggs in uteri were operculate, ovoid to ellipsoid, and 120-135×68-75 μm in size. In scanning electron microscopy, the head collar was prominent with collar spines looking like horns. Scale-like tegumental spines were densely distributed on the ventral surface between the head collar and ventral sucker. Sensory papillae were distributed mainly on the tegument around suckers. By this study, it has been first confirmed that the life cycle of A. malayanum exists in Cambodia.

Citations

Citations to this article as recorded by

Molecular detection and phylogenetic characterization of zoonotic trematodes in pigs from Northeastern India
Dipshikha Saikia, Rija Gogoi, Harshita Neog, Suman Dahal, Sudeep Ghatani
Journal of Parasitic Diseases.2026;[Epub] CrossRef
The ribosomal transcription units of five echinostomes and their taxonomic implications for the suborder Echinostomata (Trematoda: Platyhelminthes)
Thanh Hoa Le, Linh Thi Khanh Pham, Dong Van Quyen, Khue Thi Nguyen, Huong Thi Thanh Doan, Weerachai Saijuntha, David Blair
Parasitology Research.2024;[Epub] CrossRef
Biological Hazards and Indicators Found in Products of Animal Origin in Cambodia from 2000 to 2022: A Systematic Review
Shwe Phue San, Rortana Chea, Delia Grace, Kristina Roesel, Sothyra Tum, Stephen Young, Tumnoon Charaslertrangsi, Nazanin Zand, Shetty Seetharama Thombathu, Ra Thorng, Leab Kong, Kuok Fidero, Linda Nicolaides
International Journal of Environmental Research and Public Health.2024; 21(12): 1621. CrossRef
Mitophylogenomics of the zoonotic fluke Echinostoma malayanum confirms it as a member of the genus Artyfechinostomum Lane, 1915 and illustrates the complexity of Echinostomatidae systematics
Linh Thi Khanh Pham, Weerachai Saijuntha, Scott P. Lawton, Thanh Hoa Le
Parasitology Research.2022; 121(3): 899. CrossRef
High diversity of trematode metacercariae that parasitize freshwater gastropods in Bangkok, Thailand, and their infective situations, morphologies and phylogenetic relationships
Pichit Wiroonpan, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
Parasitology.2022; 149(7): 913. CrossRef
General overview of the current status of human foodborne trematodiasis
Jong-Yil Chai, Bong-Kwang Jung
Parasitology.2022; 149(10): 1262. CrossRef
Foodborne intestinal flukes: A brief review of epidemiology and geographical distribution
Jong-Yil Chai, Bong-Kwang Jung
Acta Tropica.2020; 201: 105210. CrossRef

11,657 View
154 Download
7 Web of Science
Crossref

Original Articles

Subsets of Inflammatory Cytokine Gene Polymorphisms are Associated with Risk of Carcinogenic Liver Fluke Opisthorchis viverrini-Associated Advanced Periductal Fibrosis and Cholangiocarcinoma: Arpa Surapaitoon, Sutas Suttiprapa, Eimorn Mairiang, Narong Khuntikeo, Chawalit Pairojkul, Jeffrey Bethony, Paul J. Brindley, Banchob Sripa; Korean J Parasitol 2017;55(3):295-304.
Published online June 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.3.295

Abstract
PDF

Opisthorchis viverrini infection induces chronic inflammation, and a minor proportion of infected individuals develop advanced periductal fibrosis (APF) and cholangiocarcinoma (CCA). Inflammatory cytokines and/or their gene polymorphisms may link to these biliary pathologies. We therefore investigated associations among cytokine gene polymorphisms and cytokine production in 510 Thai cases infected with O. viverrini who presented with APF+ or APF-, as established by abdominal ultrasonography as well as in patients diagnosed with CCA. Levels of pro-inflammatory and anti-inflammatory cytokines were determined in culture supernatants after stimulation of peripheral blood mononuclear cells (PBMCs) with O. viverrini excretory-secretory (ES) products. Pro-inflammatory cytokines, IL-1β, IL-6, IFN-γ, LT-α, and TNF-α were significantly increased in CCA patients compared with non-CCA (APF- and APF+) cases. Polymorphisms in genes encoding IL-1β -511C/T, IL-6 -174G/C, IFN-γ +874T/A, LT-α +252A/G, and TNF-α -308G/A were then investigated by using PCR-RFLP or allele specific-PCR (AS-PCR) analyses. In the CCA cases, LT-α +252A/G and TNF-α -308G/A heterozygous and homozygous variants showed significantly higher levels of these cytokines than the wild type. By contrast, levels of cytokines in wild type of IFN-γ +874T/A were significantly higher than the variants in CCA cases. IFN-γ +874T/A polymorphisms were associated with advanced periductal fibrosis, whereas IL-6 -174G/C polymorphisms were associated with CCA. To our knowledge, these findings provide the first demonstration that O. viverrini infected individuals carrying several specific cytokine gene polymorphisms are susceptible to develop fibrosis and CCA.

Citations

Citations to this article as recorded by

Liver flukes and kidney injury: systematic review of human and animal data (from 1950 to 2025)
A. Kovner, Y. Kapushchak, M. Pakharukova
Journal of Helminthology.2026;[Epub] CrossRef
Current Status of Drug Treatment of Cholangiocarcinoma—Updated Progress and Critical Limitations
Jennifer Cillis, Courtney Chen, Supriya Deshpande, Yuman Fong, Shyambabu Chaurasiya
Pharmaceuticals.2026; 19(4): 554. CrossRef
The Search for Risk, Diagnostic, and Prognostic Biomarkers of Cholangiocarcinoma and Their Biological and Clinicopathologic Significance
Rocio I.R. Macias, Hiroaki Kanzaki, Carmen Berasain, Matias A. Avila, Jose J.G. Marin, Yujin Hoshida
The American Journal of Pathology.2025; 195(3): 422. CrossRef
Gut Microbiome and Bile Acid Interactions
Nan Wu, Sareh Bayatpour, Phillip B. Hylemon, Sayed O. Aseem, Paul J. Brindley, Huiping Zhou
The American Journal of Pathology.2025; 195(3): 397. CrossRef
Role of interleukins in the pathogenesis of cholangiocarcinoma: A literature review
Saira Rafaqat, Hafsa Hamid, Roha Asif, Muhammad Asif, Maria Tariq, Malaika Saleem, Hijab Abaid
World Journal of Gastrointestinal Oncology.2025;[Epub] CrossRef
Multiomics analysis of neutrophils in SLE: insights from adult and paediatric disease
Grace Filbertine, Isobel Kynoch, Genna A Abdullah, Lucy Gill, Rudi Grosman, Marie M Phelan, Zoe McLaren, Tawatchai Deekajorndech, Direkrit Chiewchengchol, Nattiya Hirankarn, Helen L Wright
Clinical and Experimental Immunology.2025;[Epub] CrossRef
Associations of Liver Fluke Infection and Cholangiocarcinoma: A Scoping Review
Ankitha Sivanand, Durva Talati, Yash Kalariya, Priyansh Patel, Siddharth Kamal Gandhi
Cureus.2023;[Epub] CrossRef
Gene polymorphisms of inflammatory factors in liver cirrhosis
Sailan Xiao, Xiongfeng Pan, Xun Huang, Yamin Liu, Shi Wu Wen, Aizhong Liu
Frontiers in Genetics.2023;[Epub] CrossRef
Genotypic and allelic distribution of IFN‐γ +874T/A and TGF‐β1 −509C/T single‐nucleotide polymorphisms in human immunodeficiency virus‐infected Thais
Chareeporn Akekawatchai, Chada Phuegsilp, Khaimuk Changsri, Thanawan Soimanee, Warisara Sretapunya
Journal of Medical Virology.2022; 94(6): 2882. CrossRef
What Do In Vitro and In Vivo Models Tell Us about Anisakiasis? New Tools Still to Be Explored
Serena Cavallero, Ilaria Bellini, Antonella Pizzarelli, Stefano D’Amelio
Pathogens.2022; 11(3): 285. CrossRef
Inflammatory Response in Caco-2 Cells Stimulated with Anisakis Messengers of Pathogenicity
Ilaria Bellini, Daniela Scribano, Meysam Sarshar, Cecilia Ambrosi, Antonella Pizzarelli, Anna Teresa Palamara, Stefano D’Amelio, Serena Cavallero
Pathogens.2022; 11(10): 1214. CrossRef
The Role of Single-Nucleotide Polymorphisms in Cholangiocarcinoma: A Systematic Review
Guanwu Wang, Lara Rosaline Heij, Dong Liu, Edgar Dahl, Sven Arke LANG, Tom Florian Ulmer, Tom LUEDDE, Ulf Peter Neumann, Jan Bednarsch
Cancers.2022; 14(23): 5969. CrossRef
Improvement of a PCR-based method for the detection of Opisthorchis viverrini eggs in human stool samples by targeting internal transcribed spacer-2 (ITS-2), cytochrome oxidase subunit 1 (cox1), and cytochrome b (cyb)
Supaporn Pumpa, Wansika Phadungsil, Rudi Grams, Pongsakorn Martviset, Toon Ruang-Areerate, Mathirut Mungthin, Amornrat Geadkaew-Krenc
Journal of Parasitic Diseases.2021; 45(2): 474. CrossRef
In vitro cytotoxic and toxicological activities of ethanolic extract of Kaempferia galanga Linn. and its active component, ethyl-p-methoxycinnamate, against cholangiocarcinoma
Porwornwisit Tritripmongkol, Tullayakorn Plengsuriyakarn, Mayuri Tarasuk, Kesara Na-Bangchang
Journal of Integrative Medicine.2020; 18(4): 326. CrossRef
Fungal and Parasitic Infections of the Liver
Sirina Ekpanyapong, K. Rajender Reddy
Gastroenterology Clinics of North America.2020; 49(2): 379. CrossRef

10,720 View
166 Download
18 Web of Science
Crossref

Codon Usage Patterns of Tyrosinase Genes in Clonorchis sinensis: Young-An Bae; Korean J Parasitol 2017;55(2):175-183.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.175

Abstract
PDF

Codon usage bias (CUB) is a unique property of genomes and has contributed to the better understanding of the molecular features and the evolution processes of particular gene. In this study, genetic indices associated with CUB, including relative synonymous codon usage and effective numbers of codons, as well as the nucleotide composition, were investigated in the Clonorchis sinensis tyrosinase genes and their platyhelminth orthologs, which play an important role in the eggshell formation. The relative synonymous codon usage patterns substantially differed among tyrosinase genes examined. In a neutrality analysis, the correlation between GC₁₂ and GC₃ was statistically significant, and the regression line had a relatively gradual slope (0.218). NC-plot, i.e., GC₃ vs effective number of codons (ENC), showed that most of the tyrosinase genes were below the expected curve. The codon adaptation index (CAI) values of the platyhelminth tyrosinases had a narrow distribution between 0.685/0.714 and 0.797/0.837, and were negatively correlated with their ENC. Taken together, these results suggested that CUB in the tyrosinase genes seemed to be basically governed by selection pressures rather than mutational bias, although the latter factor provided an additional force in shaping CUB of the C. sinensis and Opisthorchis viverrini genes. It was also apparent that the equilibrium point between selection pressure and mutational bias is much more inclined to selection pressure in highly expressed C. sinensis genes, than in poorly expressed genes.

Citations

Citations to this article as recorded by

Analysis of codon usage bias in mitochondrial CO gene among platyhelminthes
Gulshana A. Mazumder, Arif Uddin, Supriyo Chakraborty
Molecular and Biochemical Parasitology.2021; 245: 111410. CrossRef

15,204 View
136 Download
1 Web of Science
Crossref

Development of Urinary Bladder Pre-Neoplasia by Schistosoma haematobium Eggs and Chemical Carcinogen in Mice: Bayissa Chala, Min-Ho Choi, Kyung Chul Moon, Hyung Suk Kim, Cheol Kwak, Sung-Tae Hong; Korean J Parasitol 2017;55(1):21-29.
Published online February 28, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.1.21

Abstract
PDF

Schistosoma haematobium is a biocarcinogen of human urinary bladder (UB). The present study investigated developing UB cancer mouse model by injecting S. haematobium eggs into the bladder wall and introduction of chemical carcinogens. Histopathological findings showed mild hyperplasia to epithelial vacuolar change, and high grade dysplasia. Squamous metaplasia was observed in the S. haematobium eggs+NDMA group at week 12 but not in other groups. Immunohistochemistry revealed significantly high expression of Ki-67 in urothelial epithelial cells of the S. haematobium eggs+BBN group at week 20. The qRT-PCR showed high expression of p53 gene in S. haematobium eggs group at week 4 and S. haematobium eggs+BBN group at week 20. E-cadherin and vimentin showed contrasting expression in S. haematobium eggs+BBN group. Such inverse expression of E-cadherin and vimentin may indicate epithelial mesenchymal transition in the UB tissue. In conclusion, S. haematobium eggs and nitrosamines may transform UB cells into squamous metaplasia and dysplasia in correlation with increased expression of Ki-67. Marked decrease in E-cadherin and increase in p53 and vimentin expressions may support the transformation. The present study introduces a promising modified animal model for UB cancer study using S. haematobium eggs.

Citations

Citations to this article as recorded by

Reinventing type 2 immunity in cancer
Marek Wagner, Hiroyoshi Nishikawa, Shigeo Koyasu
Nature.2025; 637(8045): 296. CrossRef
Effects of Schistosoma haematobium infection and treatment on the systemic and mucosal immune phenotype, gene expression and microbiome: A systematic review
Anna M. Mertelsmann, Sheridan F. Bowers, Drew Wright, Jane K. Maganga, Humphrey D. Mazigo, Lishomwa C. Ndhlovu, John M. Changalucha, Jennifer A. Downs, Hamed Kalani
PLOS Neglected Tropical Diseases.2024; 18(9): e0012456. CrossRef
Development of an automated artificial intelligence-based system for urogenital schistosomiasis diagnosis using digital image analysis techniques and a robotized microscope
Carles Rubio Maturana, Allisson Dantas de Oliveira, Francesc Zarzuela, Edurne Ruiz, Elena Sulleiro, Alejandro Mediavilla, Patricia Martínez-Vallejo, Sergi Nadal, Tomàs Pumarola, Daniel López-Codina, Alberto Abelló, Elisa Sayrol, Joan Joseph-Munné, David J
PLOS Neglected Tropical Diseases.2024; 18(11): e0012614. CrossRef
Roles of microRNAs and Long Non-Coding RNAs Encoded by Parasitic Helminths in Human Carcinogenesis
Ana Gabriela Leija-Montoya, Javier González-Ramírez, Gustavo Martínez-Coronilla, María Esther Mejía-León, Mario Isiordia-Espinoza, Fausto Sánchez-Muñoz, Elda Georgina Chávez-Cortez, Viviana Pitones-Rubio, Nicolas Serafín-Higuera
International Journal of Molecular Sciences.2022; 23(15): 8173. CrossRef
The role of helminths in the development of non-communicable diseases
Yifan Wu, Megan Duffey, Saira Elizabeth Alex, Charlie Suarez-Reyes, Eva H. Clark, Jill E. Weatherhead
Frontiers in Immunology.2022;[Epub] CrossRef
Pathogens and Carcinogenesis: A Review
Muhammad Nur Adam Hatta, Ezanee Azlina Mohamad Hanif, Siok-Fong Chin, Hui-min Neoh
Biology.2021; 10(6): 533. CrossRef
E-cadherin and N-cadherin Immunohistochemical Expression in Proliferating Urothelial Lesions: Potential Novel Cancer Predictive EMT Profiles
Lobna S. Shash, Riham A. Ibrahim, Shimaa A. Elgohary
Applied Immunohistochemistry & Molecular Morphology.2021; 29(9): 657. CrossRef
Parasite–bacteria interrelationship
Dalia S. Ashour, Ahmad A. Othman
Parasitology Research.2020; 119(10): 3145. CrossRef
Urinary schistosomiasis and the associated bladder cancer: update
Mohamed S. Zaghloul, Tarek M. Zaghloul, Mai K. Bishr, Brian C. Baumann
Journal of the Egyptian National Cancer Institute.2020;[Epub] CrossRef
Differential responses of epithelial cells from urinary and biliary tract to eggs of Schistosoma haematobium and S. mansoni
Rafael Nacif-Pimenta, Alessandra da Silva Orfanó, Ilana A. Mosley, Shannon E. Karinshak, Kenji Ishida, Victoria H. Mann, Paulo Marcos Zech Coelho, José M. Correia da Costa, Michael H. Hsieh, Paul J. Brindley, Gabriel Rinaldi
Scientific Reports.2019;[Epub] CrossRef
Cytological and Wet Mount Microscopic Observations Made in Urine ofSchistosoma haematobium-Infected Children: Hint of the Implication in Bladder Cancer
Patience B. Tetteh-Quarcoo, Benjamin K. Akuetteh, Irene A. Owusu, Solomon E. Quayson, Simon K. Attah, Robert Armah, Emmanuel Afutu, Ama Afrah, Kantanka Addo-Osafo, Cecilia Smith, Richard K. Gyasi, Patrick F. Ayeh-Kumi
Canadian Journal of Infectious Diseases and Medical Microbiology.2019; 2019: 1. CrossRef
Neglected Agent Eminent Disease: Linking Human Helminthic Infection, Inflammation, and Malignancy
Naina Arora, Rimanpreet Kaur, Farhan Anjum, Shweta Tripathi, Amit Mishra, Rajiv Kumar, Amit Prasad
Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
Understanding Urogenital Schistosomiasis-Related Bladder Cancer: An Update
Kenji Ishida, Michael H. Hsieh
Frontiers in Medicine.2018;[Epub] CrossRef
Helminth infection–induced malignancy
Paul J. Brindley, Alex Loukas, Laura J Knoll
PLOS Pathogens.2017; 13(7): e1006393. CrossRef

17,664 View
240 Download
16 Web of Science
Crossref

Electron Microscopic Alterations in Pediculus humanus capitis Exposed to Some Pediculicidal Plant Extracts: Dina M. H. El Akkad, Naglaa Saad M. El-Gebaly, Hebat-Allah Salah A. Yousof, Mousa A. M. Ismail; Korean J Parasitol 2016;54(4):527-532.
Published online August 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.4.527

Abstract
PDF

Head lice, Pediculus humanus capitis, infestation is an important public health problem in Egypt. Inadequate application of topical pediculicides and the increasing resistance to the commonly used pediculicides made the urgent need for the development of new agents able to induce irreversible changes in the exposed lice leading to their mortality. The aim of the present work is to evaluate pediculicidal efficacy of some natural products such as olive oil, tea tree oil, lemon juice, and ivermectin separately in comparison with tetramethrin-piperonyl butoxide (licid), as a standard pediculicide commonly used in Egypt. The effects of these products were evaluated by direct observation using dissecting and scanning electron microscopes (SEM). Results showed that after 1 hr exposure time in vitro, absolute (100%) mortalities were recorded after exposure to 1% ivermectin and fresh concentrate lemon juice. The mortalities were decreased to 96.7% after exposure to tea tree oil. Very low percentage of mortality (23.3%) was recorded after 1 hr of exposure to extra virgin olive oil. On the other hand, the reference pediculicide (licid) revealed only mortality rate of 93.3%. On the contrary, no mortalities were recorded in the control group exposed to distilled water. By SEM examination, control lice preserved outer smooth architecture, eyes, antenna, respiratory spiracles, sensory hairs, and legs with hook-like claws. In contrast, dead lice which had been exposed to pediculicidal products showed damage of outer smooth architecture, sensory hairs, respiratory spiracles and/or clinching claws according to pediculicidal products used.

Citations

Citations to this article as recorded by

Phytochemistry and pharmacology of Melaleuca alternifolia: Bridging aboriginal heritage with contemporary science
Nader Ebrahimi, Hadiseh Shokouhi, Marzieh Omrani, Maryam Manzari Tavakoli, Andia Vojdani, Christine Carson, Parvaneh Mehrbod, Saeid Ghavami
Biochimica et Biophysica Acta (BBA) - General Subjects.2026; 1870(5): 130917. CrossRef
Head lice prevalence in suburban Bangkok, Thailand, after the COVID-19 pandemic and herbal shampoo treatment
Sinthusiri, J., Sittichok, S., Passara, H., Soonwera, M.
International Journal of Agricultural Technology.2026; 22(1): 515. CrossRef
Australian Tea Tree (Melaleuca alternifolia) oil: an updated review of antimicrobial and other medicinal properties
Andrea Bugarcic, Esther Joy Bowles, Kate Summer, Tamara Agnew, Bronwyn Barkla, Romy Lauche
Phytomedicine Plus.2025; 5(3): 100846. CrossRef
Toxicity of 6-gingerol and Cymbopogon citratus against Pediculus humanus capitis De Geer (Phthiraptera: Pediculidae): Mortality, detoxifying enzymes, and morphological ultrastructure alterations in lice
Rizal Subahar, Rizqy Hadyansyah, Rachmanin Aldilla, Yulhasri Yulhasri, Rawina Winita, Surya Dwira, Gulshan Fahmi El Bayani
Research in Veterinary Science.2024; 177: 105364. CrossRef
D‐limonene nanoemulsion: lousicidal activity, stability, and effect on the cuticle of Columbicola columbae
Sahar M. Gadelhaq, Shawky M. Aboelhadid, Abdel‐Azeem S. Abdel‐Baki, Khaled M. Hassan, Waleed M. Arafa, Samar M. Ibrahium, Saleh Al‐Quraishy, Ahmed O. Hassan, Shams G. Abd El‐Kareem
Medical and Veterinary Entomology.2023; 37(1): 63. CrossRef
Ovicidal and Pediculicidal Activity of Indigofera suffruticosa Mill. Leaf Oil on Pediculus humanus capitis Egg to Adult Stages
Patwarapohn Udonsan, Ratchadawan Aukkanimart, Jatuporn Prathumtet, Somchai Butnan, Pranee Sriraj
Acta Parasitologica.2023; 68(4): 853. CrossRef
Biochemical constituents and insecticidal activities of Callistemon viminalis essential oil against adults and eggs of Pediculus humanus capitis (Phthiraptera: Pediculidae)
Manachai Yingklang, Aroonsri Priprem, Natsajee Nualkaew, Bunleu Sungthong, Porntip Pinlaor, Somchai Pinlaor
Phytomedicine Plus.2022; 2(1): 100156. CrossRef
Antiparasitic Activity of Tea Tree Oil (TTO) and Its Components against Medically Important Ectoparasites: A Systematic Review
Solomon Abrha Bezabh, Wubshet Tesfaye, Julia K. Christenson, Christine F. Carson, Jackson Thomas
Pharmaceutics.2022; 14(8): 1587. CrossRef
Unravelling the anthelmintic bioactives from Jasminum grandiflorum L. subsp. Floribundum adopting in vitro biological assessment
Dorria Hussein, Riham A. El-Shiekh, Fatema R. Saber, Marwa M. Attia, Mohamed R. Mousa, Attia H. Atta, Essam Abdel-Sattar, Samar M. Mouneir
Journal of Ethnopharmacology.2021; 275: 114083. CrossRef
In vitro experiments of Pediculus humanus capitis (Phthiraptera: Pediculidae) resistance to permethrin and 6-paradol in East Jakarta: Detoxification enzyme activity and electron microscopic changes in lice
Rizal Subahar, Lisawati Susanto, Rachmanin Aidilla, Annisa Putri Aulia, Yulhasri Yulhasri, Rawina Winita, Nadar S. Lubis, Ika Puspa Sari
Veterinary World.2021; : 3065. CrossRef
Ovicidal effect of essential oils from Zingiberaceae plants and Eucalytus globulus on eggs of head lice, Pediculus humanus capitis De Geer
Mayura Soonwera, Orawan Wongnet, Sirawut Sittichok
Phytomedicine.2018; 47: 93. CrossRef

10,555 View
208 Download
8 Web of Science
Crossref

Increased Expression of TGF-β1 in Correlation with Liver Fibrosis during Echinococcus granulosus Infection in Mice: Yumei Liu, Gulizhaer Abudounnasier, Taochun Zhang, Xuelei Liu, Qian Wang, Yi Yan, Jianbing Ding, Hao Wen, Delixiati Yimiti, Xiumin Ma; Korean J Parasitol 2016;54(4):519-525.
Published online August 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.4.519

Abstract
PDF

To investigate the potential role of transforming growth factor (TGF)-β1 in liver fibrosis during Echinococcus granulosus infection, 96 BALB/c mice were randomly divided into 2 groups, experimental group infected by intraperitoneal injection with a metacestode suspension and control group given sterile physiological saline. The liver and blood samples were collected at days 2, 8, 30, 90, 180, and 270 post infection (PI), and the expression of TGF-β1 mRNA and protein was determined by real-time quantitative RT-PCR and ELISA, respectively. We also evaluated the pathological changes in the liver during the infection using hematoxylin and eosin (H-E) and Masson staining of the liver sections. Pathological analysis of H-E stained infected liver sections revealed liver cell edema, bile duct proliferation, and structural damages of the liver as evidenced by not clearly visible lobular architecture of the infected liver, degeneration of liver cell vacuoles, and infiltration of lymphocytes at late stages of infection. The liver tissue sections from control mice remained normal. Masson staining showed worsening of liver fibrosis at the end stages of the infection. The levels of TGF-β1 did not show significant changes at the early stages of infection, but there were significant increases in the levels of TGF-β1 at the middle and late stages of infection (P<0.05). RT-PCR results showed that, when compared with the control group, TGF-β1 mRNA was low and comparable with that in control mice at the early stages of infection, and that it was significantly increased at day 30 PI and remained at high levels until day 270 PI (P<0.05). The results of this study suggested that increased expression of TGF-β1 during E. granulosus infection may play a significant role in liver fibrosis associated with E. granulosus infection.

Citations

Citations to this article as recorded by

Inhibition of the MyD88 signaling pathway could upregulates Ghrelin expression to synergistically regulate hepatic Echinococcus multilocularis-infected progression
Jiang Zhu, Tanfang Zhou, Guangfeng Chen, Yuhui Wu, Xia Chen, Ya Song, Ayinula Tuohetali, Huijing Gao, Dongming Pang, Hao Wen, Kalibixiati Aimulajiang
Frontiers in Immunology.2024;[Epub] CrossRef
Ghrelin regulating liver activity and its potential effects on liver fibrosis and Echinococcosis
Jiang Zhu, Tanfang Zhou, Meng Menggen, Kalibixiati Aimulajiang, Hao Wen
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Characterization of the immunosuppressive environment induced by larval Echinococcus granulosus during chronic experimental infection
Leticia Grezzi, Yamila E. Martínez, Anabella A. Barrios, Álvaro Díaz, Cecilia Casaravilla, De'Broski R. Herbert
Infection and Immunity.2024;[Epub] CrossRef
Ghrelin is involved in regulating the progression of Echinococcus Granulosus-infected liver lesions through suppression of immunoinflammation and fibrosis
Jiang Zhu, Hongqiong Zhao, Aili Aierken, Tanfang Zhou, Meng Menggen, Huijing Gao, Rongdong He, Kalibixiati Aimulajiang, Hao Wen, Fernando Lopes
PLOS Neglected Tropical Diseases.2024; 18(10): e0012587. CrossRef
The expression of cytokeratin and apoptosis-related molecules in echinococcosis related liver injury
H.C. Yang, Z.K. Xing, H. Shao, X.W. Tan, E.Q. Wang, Y. Liao, H.J. Chen, X.W. Wu, X.L. Chen, S.J. Zhang
Molecular and Biochemical Parasitology.2022; 248: 111455. CrossRef
A combination of pirfenidone and TGF-β inhibition mitigates cystic echinococcosis-associated hepatic injury
Erqiang Wang, Zhenyu Liao, Lianghai Wang, Yuan Liao, Xiaodan Xu, Ping Liu, Xian Wang, Jun Hou, Huijiao Jiang, Xiangwei Wu, Xueling Chen
Parasitology.2021; 148(7): 767. CrossRef
CD3/TCRE Expression and Immunoregulatory Milieu Induced in a Secondary Intermediate Host by Different Phases of Hydatid Cyst
Alya Mashaal, Al-Shaimaa Mohsen Sadek
Acta Parasitologica.2021; 66(4): 1490. CrossRef
Pu‐erh tea extraction alleviates intestinal inflammation in mice with flora disorder by regulating gut microbiota
Zhifang Zhang, Fei He, Weixing Yang, Li Yang, Siqi Huang, Hongling Mao, Yan Hou, Rong Xiao
Food Science & Nutrition.2021; 9(9): 4883. CrossRef
Expansion of Host Regulatory T Cells by Secreted Products of the Tapeworm Echinococcus multilocularis
Justin Komguep Nono, Manfred B. Lutz, Klaus Brehm
Frontiers in Immunology.2020;[Epub] CrossRef
Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers
Federica Fratini, F. Tamarozzi, G. Macchia, L. Bertuccini, M. Mariconti, C. Birago, A. Iriarte, E. Brunetti, CM. Cretu, O. Akhan, M. Siles-Lucas, A. Díaz, Adriano Casulli, Hector H. Garcia
PLOS Neglected Tropical Diseases.2020; 14(10): e0008586. CrossRef
Subcutaneous Inoculation of Echinococcus multilocularis Induces Delayed Regeneration after Partial Hepatectomy
Shadike Apaer, Tuerhongjiang Tuxun, Heng Zhang, Amina Aierken, Tao Li, Jin-Ming Zhao, Hao Wen
Scientific Reports.2019;[Epub] CrossRef
Mechanism of Fibrosis Induced by Echinococcus spp.
Fuqiu Niu, Shigui Chong, Mingqun Qin, Shenmei Li, Riming Wei, Yumin Zhao
Diseases.2019; 7(3): 51. CrossRef
Echinococcus multilocularis inoculation induces NK cell functional decrease through high expression of NKG2A in C57BL/6 mice
Abuduaini Abulizi, Yingmei Shao, Tuerganaili Aji, Zhide Li, Chuanshan Zhang, Abudusalamu Aini, Hui Wang, Tuerhongjiang Tuxun, Liang Li, Ning Zhang, Renyong Lin, Hao Wen
BMC Infectious Diseases.2019;[Epub] CrossRef
Study on the association between TGF‑β1 and liver fibrosis in patients with hepatic cystic echinococcosis
Fengming Tian, Yumei Liu, Jian Gao, Ning Yang, Xiaoqian Shang, Jie Lv, Derong Ba, Xuan Zhou, Chuntao Zhang, Xiumin Ma
Experimental and Therapeutic Medicine.2019;[Epub] CrossRef
Granulomatous responses in larval taeniid infections
Á. Díaz, C. Sagasti, C. Casaravilla
Parasite Immunology.2018;[Epub] CrossRef
The role of oxymatrine in regulating TGF-β1 in rats with hepatic fibrosis
Jing Wu, Lin Pan, Xueqin Jin, Weihua Li, Hongbing Li, Jianmao Chen, Wen Yang
Acta Cirurgica Brasileira.2018; 33(3): 207. CrossRef
Recent advances in understanding liver fibrosis: bridging basic science and individualized treatment concepts
Ralf Weiskirchen, Sabine Weiskirchen, Frank Tacke
F1000Research.2018; 7: 921. CrossRef
Immunology of cystic echinococcosis (hydatid disease)
Álvaro Díaz
British Medical Bulletin.2017;[Epub] CrossRef

10,480 View
159 Download
17 Web of Science
Crossref

Characteristics of Imported Malaria and Species of Plasmodium Involved in Shandong Province, China (2012-2014): Chao Xu, Qing-Kuan Wei, Jin Li, Ting Xiao, Kun Yin, Chang-Lei Zhao, Yong-Bin Wang, Xiang-Li Kong, Gui-Hua Zhao, Hui Sun, Xin Liu, Bing-Cheng Huang; Korean J Parasitol 2016;54(4):407-414.
Published online August 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.4.407

Abstract
PDF

Malaria remains a serious public health problem in Shandong Province, China; therefore, it is important to explore the characteristics of the current malaria prevalence situation in the province. In this study, data of malaria cases reported in Shandong during 2012-2014 were analyzed, and Plasmodium species were confirmed by smear microscopy and nested-PCR. A total of 374 malaria cases were reported, 80.8% of which were reported from 6 prefectures. Of all cases, P. falciparum was dominant (81.3%), followed by P. vivax (11.8%); P. ovale and P. malariae together accounted for 6.4% of cases. Notably, for the first time since 2012, no indigenous case had been reported in Shandong Province, a situation that continued through 2014. Total 95.2% of cases were imported from Africa. The ratio of male/female was 92.5:1, and 96.8% of cases occurred in people 20-54 years of age. Farmers or laborers represented 77.5% of cases. No significant trends of monthly pattern were found in the reported cases. All patients were in good condition after treatment, except for 3 who died. These results indicate that imported malaria has increased significantly since 2012 in Shandong Province, especially for P. falciparum, and there is an emergence of species diversity.

Citations

Citations to this article as recorded by

Estimating the burden of imported malaria in the Asia Pacific region: a systematic review and meta-analysis
Kinley Penjor, Binay Thapa, Tsheten Tsheten, Soumya Mazumdar, Nasser Bagheri, Manas Kotepui, Kinley Wangdi
Journal of Travel Medicine.2026;[Epub] CrossRef
Toward the Elimination of Malaria in China: A Retrospective Analysis of Malaria-Endemic Characteristics and Prevention Effects in Yantai, Shandong Province, 1951 to 2017
Xiao Song, Yang Wang, Xiangli Kong, Haifang Wang, Xiaodan Huang, Hongmei Liu, Lijuan Liu, Xiuxia Guo, Chongxing Zhang, Yuqiang Zhao, Jingxuan Kou, Huaiwei Wang, Peng Cheng, Maoqing Gong
Vector-Borne and Zoonotic Diseases.2020; 20(3): 197. CrossRef
The impact of imported malaria by gold miners in Roraima: characterizing the spatial dynamics of autochthonous and imported malaria in an urban region of Boa Vista
Jaime Louzada, Nathália Coelho Vargas de Almeida, Joao Luiz Pereira de Araujo, Júlio Silva, Thiago M Carvalho, Ananias A Escalante, Joseli Oliveira-Ferreira
Memórias do Instituto Oswaldo Cruz.2020;[Epub] CrossRef
Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018
Yiting Xie, Kai Wu, Weijia Cheng, Tingting Jiang, Yi Yao, Mingxing Xu, Yan Yang, Huabing Tan, Jian Li
Malaria Journal.2020;[Epub] CrossRef
Misidentification of Plasmodium ovale as Plasmodium vivax malaria by a microscopic method: a meta-analysis of confirmed P. ovale cases
Manas Kotepui, Frederick Ramirez Masangkay, Kwuntida Uthaisar Kotepui, Giovanni De Jesus Milanez
Scientific Reports.2020;[Epub] CrossRef
Monitoring and evaluation of intervals from onset of fever to diagnosis before “1-3-7” approach in malaria elimination: a retrospective study in Shanxi Province, China from 2013 to 2018
Ting Wang, Shui-Sen Zhou, Jun Feng, Myo Minn Oo, Jing Chen, Chang-Fu Yan, Yi Zhang, Ping Tie
Malaria Journal.2019;[Epub] CrossRef
Surveillance of Antimalarial Resistance Pfcrt, Pfmdr1, and Pfkelch13 Polymorphisms in African Plasmodium falciparum imported to Shandong Province, China
Chao Xu, Qingkuan Wei, Kun Yin, Hui Sun, Jin Li, Ting Xiao, Xiangli Kong, Yongbin Wang, Guihua Zhao, Song Zhu, Jingxuan Kou, Ge Yan, Bingcheng Huang
Scientific Reports.2018;[Epub] CrossRef
Epidemiological analysis of 133 malaria cases in Shanxian county, Shandong Province, China
Qi-Qi Shi, Peng Cheng, Chong-Xing Zhang, Xiu-Xia Guo, Li-Juan Liu, Hai-Fang Wang, Jing-Xuan Kou, Xiao-Dan Huang, Huai-Wei Wang, Mao-Qing Gong
Asian Pacific Journal of Tropical Medicine.2017; 10(8): 802. CrossRef

12,011 View
150 Download
9 Web of Science
Crossref

Seroepidemiological Survey of Zoonotic Diseases in Small Mammals with PCR Detection of Orientia tsutsugamushi in Chiggers, Gwangju, Korea: Jung Wook Park, Jae Keun Chung, Sun Hee Kim, Sun Ju Cho, Yi Deun Ha, So Hyang Jung, Hye Jung Park, Hyun Jae Song, Jung Yoon Lee, Dong Min Kim, Jah Pyus, Dong Ryong Ha, Eun Sun Kim, Jae Il Lee; Korean J Parasitol 2016;54(3):307-313.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.307

Abstract
PDF

Serosurveillance for zoonotic diseases in small mammals and detection of chiggers, the vector of Orientia tsutsugamushi, were conducted from September 2014 to August 2015 in Gwangju Metropolitan Area. Apodemus agrarius was the most commonly collected small mammals (158; 91.8%), followed by Myodes regulus (8; 4.6%), and Crocidura lasiura (6; 3.5%). The highest seroprevalence of small mammals for O. tsutsugamushi (41; 26.3%) was followed by hantaviruses (24; 15.4%), Rickettsia spp. (22; 14.1%), and Leptospira (2; 1.3%). A total of 3,194 chiggers were collected from small mammals, and 1,236 of 3,194 chiggers were identified with 7 species of 3 genera: Leptotrombidium scutellare was the most commonly collected species (585; 47.3%), followed by L. orientale (422; 34.1%), Euchoengastia koreaensis (99; 8.0%), L. palpale (58; 4.7%), L. pallidum (36; 2.9%), Neotrombicula gardellai (28; 2.3%), and L. zetum (8; 0.6%). L. scutellare was the predominant species. Three of 1,236 chigger mites were positive for O. tsutsugamushi by PCR. As a result of phylogenetic analysis, the O. tsutsugamushi strain of chigger mites had sequence homology of 90.1-98.2% with Boryong. This study provides baseline data on the distribution of zoonotic diseases and potential vectors for the development of prevention strategies of vector borne diseases in Gwangju metropolitan area.

Citations

Citations to this article as recorded by

A Novel Strain of Orientia tsutsugamushi Detected from Chiggers (Acari: Trombiculidae) on Wild Rodents
Hak Seon Lee, Seong Yoon Kim, Hee Il Lee
Pathogens.2025; 14(1): 29. CrossRef
Geographic distribution of Orientia tsutsugamushi strains on chigger mites in the Republic of Korea (2021–2023)
Hyeon Seung Lee, Byung-Eon Noh, Hyunwoo Kim, Heeil Lee
Parasites & Vectors.2025;[Epub] CrossRef
First Report on the Molecular Detection and Characterization of Rickettsia felis in Laelapidae (Acari: Mesostigmata) Mites in Malaysia
Hiryahafira Mohamad Tahir, Faraliana Che Lah Ernieenor, Suhaili Zainal Abidin, Vishalani Vishnu Narainasamy, Mariana Ahamad
Veterinary Sciences.2025; 12(5): 443. CrossRef
Detection of Orientia tsutsugamushi in Novel Trombiculid Mite Species in Northern Tamil Nadu, India: Use of Targeting the Multicopy traD Gene
John Antony Jude Prakash, Kannan Kamarasu, P Philip Samuel, Renu Govindarajan, Punitha Govindasamy, Liji Anna Johnson, P Ramalingam, J Nirmalson, K C Seran, Kevin Macaluso
Journal of Medical Entomology.2022; 59(2): 693. CrossRef
Emerging hantavirus infection in wild rodents captured in suburbs of Gwangju Metropolitan City, South Korea
Mi hee Seo, Choon-Mee Kim, Dong-Min Kim, Na Ra Yun, Jung Wook Park, Jae Keun Chung, Wen-Ping Guo
PLOS Neglected Tropical Diseases.2022; 16(6): e0010526. CrossRef
Orientia tsutsugamushi in Chiggers and Small Mammals in Laos
Ivo Elliott, Rawadee Kumlert, Neeranuch Thangnimitchok, Stuart D. Blacksell, Ampai Tanganuchitcharnchai, Daniel H. Paris, Paul N. Newton, Serge Morand
Vector-Borne and Zoonotic Diseases.2022; 22(10): 505. CrossRef
Nationwide Incidence of Chigger Mite Populations and Molecular Detection of Orientia tsutsugamushi in the Republic of Korea, 2020
Min-Goo Seo, Bong-Goo Song, Tae-Kyu Kim, Byung-Eon Noh, Hak Seon Lee, Wook-Gyo Lee, Hee Il Lee
Microorganisms.2021; 9(8): 1563. CrossRef
First report of Borrelia burgdorferi sensu stricto detection in a commune genospecies in Apodemus agrarius in Gwangju, South Korea
Choon Mee Kim, So Young Park, Dong-Min Kim, Jung Wook Park, Jae Keun Chung
Scientific Reports.2021;[Epub] CrossRef
Epidemiological Characteristics of Rodents and Chiggers with Orientia Tsutsugamushi in the Republic of Korea
Jung Wook Park, Dae Sung Yu, Gi Seong Lee, Jin Jong Seo, Jae Keun Chung, Jae Il Lee
The Korean Journal of Parasitology.2020; 58(5): 559. CrossRef
Molecular Epidemiology of an Orientia tsutsugamushi Gene Encoding a 56-kDa Type-Specific Antigen in Chiggers, Small Mammals, and Patients from the Southwest Region of Korea
Jung Wook Park, Sun Hee Kim, Duck Woong Park, So Hyang Jung, Hye Jung Park, Mi Hee Seo, Hyeon Je Song, Jung Yoon Lee, Dong Min Kim, Choon-Mee Kim, Byong Chul Gill, Hang Jin Jeong, Jeong Min Lee, Dong Ryong Ha, Eun Sun Kim, Jae Keun Chung
The American Journal of Tropical Medicine and Hygiene.2018; 98(2): 616. CrossRef

10,767 View
145 Download
10 Web of Science
Crossref

C3H/He Mice as an Incompatible Cholangiocarcinoma Model by Clonorchis sinensis, Dicyclanil and N-Nitrosodimethylamine: Md. Hafiz Uddin, Shunyu Li, Yan Jin, Min-Ho Choi, Ja June Jang, Sung-Tae Hong; Korean J Parasitol 2016;54(3):281-289.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.281

Clonorchis sinensis is a Group-I bio-carcinogen, associated with cholangiocarcinoma (CCA). The hamster is the only experimental model of C. sinensis-mediated CCA, but we oblige another animal model. The present study intended to develop a C. sinensis (Cs) mediated CCA model using C3H/He mice, co-stimulated with N-nitrosodimethyl-amine (NDMA) and dicyclanil (DC). The mice were divided into 8 groups with different combinations of Cs, NDMA, and DC. Six months later the mice were sacrificed and subjected to gross and histopathological examination. The body weights were significantly reduced among the groups treated with 2 or more agents (eg. Cs+NDMA, Cs+DC, NDMA+DC, and Cs+NDMA+DC). In contrast, liver weight percentages to body weight were increased in above groups by 4.1% to 4.7%. A Change of the spleen weight was observed only in Cs+NDMA group. Though C. sinensis infection is evident from hyperplastic changes, only 1 worm was recovered. T wo mice, 1 from Cs and the other from Cs+DC group, showed mass forming lesions; 1 (281.2 mm3) from the Cs group was a hepatocellular adenoma and the other (280.6 mm3) from the Cs+DC group was a cystic mass (peliosis). Higher prevalence of gray-white nodules was observed in Cs group (42.9%) followed by Cs+NDMA+DC group (21.4%). The mice of the Cs+NDMA+DC group showed hyper-proliferation of the bile duct with fibrotic changes. No characteristic change for CCA was recognized in any of the groups. In conclusion, C3H/He mice produce no CCA but extensive fibrosis when they are challenged by Cs, NDMA, and DC together.

Citations

Citations to this article as recorded by

Clonorchis sinensis and Cholangiocarcinoma
Eun-Min Kim, Sung-Tae Hong
Journal of Korean Medical Science.2025;[Epub] CrossRef
Study of gut microbiota in cholangiocarcinoma patients
O. S. Fedorova, A. Е. Kovshirina, T. S. Sokolova, V. V. Kulenich, L. M. Ogorodova
Bulletin of Siberian Medicine.2025; 24(1): 105. CrossRef
Exploratory study on the role of Clonorchis sinensis infection in promoting cholangiocarcinoma progression
Shitao Li, Yiqi Jiang, Jun Kawanokuchi, Xueling Deng, Yuhong Wu, Yu Chen, Lixia Zeng, Ganghuan Deng, Damian Li, Tingzheng Zhan, Dengyu Liu, Ning Ma, Zeli Tang
Parasites & Vectors.2025;[Epub] CrossRef
Opisthorchis Felineus Infection is a Risk Factor for Cholangiocarcinoma in Western Siberia: A Hospital-based Case-control Study
Olga S Fedorova, Anna E Kovshirina, Yulia V Kovshirina, Jan Hattendorf, Sergey V Onishchenko, Ludmila L Katanakhova, Stanislav S Taslicki, Andrey V Chizhikov, Ilya A Tataurov, Sergey V Vtorushin, Banchob Sripa, Ludmila M Ogorodova, Peter Odermatt
Clinical Infectious Diseases.2023; 76(3): e1392. CrossRef
Similarities and differences among the Opisthorchiidae liver flukes: insights from Opisthorchis felineus
Maria Y. Pakharukova, Viatcheslav A. Mordvinov
Parasitology.2022; 149(10): 1306. CrossRef
The Overactivation of NADPH Oxidase during Clonorchis sinensis Infection and the Exposure to N-Nitroso Compounds Promote Periductal Fibrosis
Ji Hoon Jeong, Junyeong Yi, Myung Ki Hwang, Sung-Jong Hong, Woon-Mok Sohn, Tong-Soo Kim, Jhang Ho Pak
Antioxidants.2021; 10(6): 869. CrossRef
Association between cholangiocarcinoma and liver flukes: review of epidemiological studies
A. E. Kovshirina, O. S. Fedorova, Y. V. Kovshirina, S. V. Onishchenko, S. S. Taslicki, L. L. Katanakhova, A. V. Chizhikov, I. A. Tataurov, L. M. Ororodova
Bulletin of Siberian Medicine.2020; 19(1): 150. CrossRef
TLR2 signal influences the iNOS/NO responses and worm development in C57BL/6J mice infected with Clonorchis sinensis
Qing-Li Yang, Ji-Qing Shen, Zhi-Hua Jiang, Yun-Liang Shi, Xiao-Ling Wan, Yi-Chao Yang
Parasites & Vectors.2017;[Epub] CrossRef

11,609 View
124 Download
9 Web of Science
Crossref

Brief Communication

Ornithodoros sawaii (Ixodida: Argasidae) Larvae Collected from Hydrobates monorhis on Sogugul and Gaerin Islands, Jeollanam-do (Province), Republic of Korea: Heung-Chul Kim, Chang-Yong Choi, Young-Soo Kwon, Seok-Min Yun, Won-Ja Lee, Sung-Tae Chong, Richard G. Robbins, Terry A. Klein; Korean J Parasitol 2016;54(2):233-238.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.233

Abstract
PDF

The 65th Medical Brigade and Public Health Command District-Korea, in collaboration with the Migratory Bird Research Center, National Park Research Institute, conducted migratory bird tick surveillance at Sogugul and Gaerin Islands (small rocky bird nesting sites), Jeollanam-do (Province), Republic of Korea (ROK), on 30 July and 1 August 2009. Breeding seabirds captured by hands in their nesting burrows were banded, identified to species, and carefully examined for ticks during the nesting season. A total of 9 Ornithodoros sawaii larvae were removed from 4 adult Hydrobates monorhis (Swinhoe’s storm petrel). The identification of the larvae of O. sawaii collected from migratory seabirds were molecularly confirmed using mitochondrial 16S rDNA primer sets.

Citations

Citations to this article as recorded by

Surveillance of African swine fever infection in wildlife and environmental samples in Gangwon-do
Sangjin Ahn, Jong-Taek Kim
Korean Journal of Veterinary Service.2022; 45(1): 13. CrossRef
Detection ofRickettsia lusitaniaeAmongOrnithodoros sawaiiSoft Ticks Collected From Japanese Murrelet Seabird Nest Material From Gugul Island, Republic of Korea
Heung-Chul Kim, Ju Jiang, Jun Hang, Su Yeon Kim, Seok-Min Yun, Chang-uk Park, Miran Kim, Sung-Tae Chong, Christina M Farris, Allen L Richards, Terry A Klein, Kevin Macaluso
Journal of Medical Entomology.2021; 58(3): 1376. CrossRef
First detection of Borrelia and Rickettsia species from Ornithodoros ticks in the Republic of Korea
Sun-Woo Han, Jeong-Byoung Chae, Young-Sun Jo, Yoon-Kyoung Cho, Jun-Gu Kang, Nam-Shik Shin, Hee-Jeong Youn, Hwa-Young Youn, Hyang-Mi Nam, Hyun-Joo Kim, Hae-Eun Kang, Joon-Seok Chae
Ticks and Tick-borne Diseases.2021; 12(4): 101689. CrossRef
Infestation of small seabirds by Ornithodoros maritimus ticks: Effects on chick body condition, reproduction and associated infectious agents
Ana Sanz-Aguilar, Ana Payo-Payo, Andreu Rotger, Lena Yousfi, Sara Moutailler, Cecile Beck, Marine Dumarest, José Manuel Igual, Miguel Ángel Miranda, Mariana Viñas Torres, Virginia Picorelli, Amandine Gamble, Thierry Boulinier
Ticks and Tick-borne Diseases.2020; 11(1): 101281. CrossRef
First Report of Newly Identified Ornithodoros Species in the Republic of Korea
Sun-Woo Han, Jeong-Byoung Chae, Young-Sun Jo, Yoon-Kyoung Cho, Jun-Gu Kang, Nam-Shik Shin, Hee-Jeong Youn, Hwa-Young Youn, Hyang-Mi Nam, Hyun-Joo Kim, Hae-Eun Kang, Joon-Seok Chae
Journal of Parasitology.2020; 106(5): 546. CrossRef

18,225 View
108 Download
9 Web of Science
Crossref

Original Articles

Inflammatory Responses in a Benign Prostatic Hyperplasia Epithelial Cell Line (BPH-1) Infected with Trichomonas vaginalis: Sang-Su Kim, Jung-Hyun Kim, Ik-Hwan Han, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2016;54(2):123-132.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.123

Abstract
PDF

Trichomonas vaginalis causes the most prevalent sexually transmitted infection worldwide. Trichomonads have been detected in prostatic tissues from prostatitis, benign prostatic hyperplasia (BPH), and prostate cancer. Chronic prostatic inflammation is known as a risk factor for prostate enlargement, benign prostatic hyperplasia symptoms, and acute urinary retention. Our aim was to investigate whether T. vaginalis could induce inflammatory responses in cells of a benign prostatic hyperplasia epithelial cell line (BPH-1). When BPH-1 cells were infected with T. vaginalis, the protein and mRNA of inflammatory cytokines, such as CXCL8, CCL2, IL-1β, and IL-6, were increased. The activities of TLR4, ROS, MAPK, JAK2/STAT3, and NF-κB were also increased, whereas inhibitors of ROS, MAPK, PI3K, NF-κB, and anti-TLR4 antibody decreased the production of the 4 cytokines although the extent of inhibition differed. However, a JAK2 inhibitor inhibited only IL-6 production. Culture supernatants of the BPH-1 cells that had been incubated with live T. vaginalis (trichomonad-conditioned medium, TCM) contained the 4 cytokines and induced the migration of human monocytes (THP-1 cells) and mast cells (HMC-1 cells). TCM conditioned by BPH-1 cells pretreated with NF-κB inhibitor showed decreased levels of cytokines and induced less migration. Therefore, it is suggested that these cytokines are involved in migration of inflammatory cells. These results suggest that T. vaginalis infection of BPH patients may cause inflammation, which may induce lower urinary tract symptoms (LUTS).

Citations

Citations to this article as recorded by

Investigating the impact of gut microbiota-derived metabolites on benign prostatic hyperplasia using network pharmacology approaches
Yuanzhao Xu, Lingyue An, Jiling Xie, Chenggong Luo, Heng Zhang, Qinyi Zhang, Guangheng Luo
BMC Pharmacology and Toxicology.2026;[Epub] CrossRef
Immunomodulatory roles of autophagic flux and IFIT in human ectocervical cells upon Trichomonas vaginalis infection
Ching-Chun Liu, Lichieh Julie Chu, Yuan-Ming Yeh, Hsin-Chung Lin, Lih-Chyang Chen, Ching-Yun Huang, Shu-Fang Chiu, Fang-Wen Cheng, Wei-Ning Lin, Kuo-Yang Huang
International Immunopharmacology.2025; 155: 114643. CrossRef
Recent Advances in Biology, Host and Microbe Interactions of the Human Sexually Transmitted Parasite Trichomonas vaginalis
Desmond L. Seybold, Gregory P. Contreras, Jia-Feng Chang, Ting-Yu Yeh
International Journal of Molecular Sciences.2025; 26(24): 12015. CrossRef
Intrinsic and extrinsic factors causing hyperplasia of the prostate
Yuki Kyoda, Kosuke Shibamori, Tetsuya Shindo, Takeshi Maehana, Kohei Hashimoto, Ko Kobayashi, Toshiaki Tanaka, Fumimasa Fukuta, Naoya Masumori
International Journal of Urology.2024; 31(7): 705. CrossRef
P. gingivalis in oral-prostate axis exacerbates benign prostatic hyperplasia via IL-6/IL-6R pathway
Shuang-Ying Wang, Yi Cai, Xiao Hu, Fei Li, Xin-Hang Qian, Ling-Yun Xia, Bo Gao, Lan Wu, Wen-Zhong Xie, Jia-Min Gu, Tong Deng, Cong Zhu, Hai-Chang Jia, Wan-Qi Peng, Jiao Huang, Cheng Fang, Xian-Tao Zeng
Military Medical Research.2024;[Epub] CrossRef
The correlation between Trichomonas vaginalis infection and reproductive system cancer: a systematic review and meta-analysis
Zhenchao Zhang, Dongxian Li, Yuhua Li, Rui Zhang, Xianghuan Xie, Yi Yao, Linfei Zhao, Xiaowei Tian, Zhenke Yang, Shuai Wang, Xuejing Yue, Xuefang Mei
Infectious Agents and Cancer.2023;[Epub] CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Trichomoniasis
Olivia T. Van Gerwen, Skye A. Opsteen, Keonte J. Graves, Christina A. Muzny
Infectious Disease Clinics of North America.2023; 37(2): 245. CrossRef
Inflammatory responses during trichomoniasis: The role of Toll‐like receptors and inflammasomes
Abdollah Jafarzadeh, Maryam Nemati, Ehsan Salarkia, Sonal Yadav, Najmeh Aminizadeh, Sara Jafarzadeh, Manisha Yadav
Parasite Immunology.2023;[Epub] CrossRef
IL-6 Signaling Link between Inflammatory Tumor Microenvironment and Prostatic Tumorigenesis
Cosmin-Victor Ene, Ilinca Nicolae, Bogdan Geavlete, Petrisor Geavlete, Corina Daniela Ene, Yun Ping Lim
Analytical Cellular Pathology.2022; 2022: 1. CrossRef
Ellagic acid improves benign prostate hyperplasia by regulating androgen signaling and STAT3
Woo Yong Park, Gahee Song, Ja Yeon Park, Kwang Seok Ahn, Hyun Jeong Kwak, Jinbong Park, Jun Hee Lee, Jae-Young Um
Cell Death & Disease.2022;[Epub] CrossRef
Association between trichomoniasis and prostate and bladder diseases: a population-based case–control study
Hung-Yi Yang, Ruei-Yu Su, Chi-Hsiang Chung, Kuo-Yang Huang, Hsin-An Lin, Jui-Yang Wang, Chien-Chou Chen, Wu-Chien Chien, Hsin-Chung Lin
Scientific Reports.2022;[Epub] CrossRef
Increased diagnostic yield of routine multiplex PCR compared to clinician requested testing for detection of Trichomonas vaginalis
Brooke Webb, Andrea Crampton, Michelle J. Francis, John Hamblin, Tony M. Korman, Maryza Graham
Pathology.2021; 53(2): 257. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang
BMC Infectious Diseases.2020;[Epub] CrossRef
Inflammatory mediators of prostate epithelial cells stimulated with Trichomonas vaginalis promote proliferative and invasive properties of prostate cancer cells
Ik‐Hwan Han, Jung‐Hyun Kim, Ki‐Seok Jang, Jae‐Sook Ryu
The Prostate.2019; 79(10): 1133. CrossRef
Monocyte-derived extracellular trap (MET) formation induces aggregation and affects motility of human spermatozoa in vitro
Mabel Schulz, Fabiola Zambrano, Hans-Christian Schuppe, Florian Wagenlehner, Anja Taubert, Ulrich Gaertner, Rául Sánchez, Carlos Hermosilla
Systems Biology in Reproductive Medicine.2019; 65(5): 357. CrossRef
Synopsis: Special Issue on “Disruption of signaling homeostasis induced crosstalk in the carcinogenesis paradigmEpistemology of the origin of cancer”
Björn L.D.M. Brücher, Ijaz S. Jamall, Obul R. Bandapalli
4open.2019; 2: 28. CrossRef
Cancer-Associated Fibroblasts Produce Netrin-1 to Control Cancer Cell Plasticity
Pei-Ju Sung, Nicolas Rama, Jeromine Imbach, Stephany Fiore, Benjamin Ducarouge, David Neves, Huei-Wen Chen, David Bernard, Pan-Chyr Yang, Agnès Bernet, Stephane Depil, Patrick Mehlen
Cancer Research.2019; 79(14): 3651. CrossRef
Atractylenolide II Induces Apoptosis of Prostate Cancer Cells through Regulation of AR and JAK2/STAT3 Signaling Pathways
Jing Wang, Moussa Ide Nasser, Salah Adlat, Ming Ming Jiang, Nan Jiang, Li Gao
Molecules.2018; 23(12): 3298. CrossRef
Proliferation of prostate epithelia induced by IL‐6 from stroma reacted with Trichomonas vaginalis
J.‐H. Kim, I.‐H. Han, Y.‐S. Kim, C.‐S. Noh, J.‐S. Ryu
Parasite Immunology.2018;[Epub] CrossRef
Proliferation of Prostate Stromal Cell Induced by Benign Prostatic Hyperplasia Epithelial Cell Stimulated WithTrichomonas vaginalisvia Crosstalk With Mast Cell
Jung-Hyun Kim, Sang-Su Kim, Ik-Hwan Han, Seobo Sim, Myoung-Hee Ahn, Jae-Sook Ryu
The Prostate.2016; 76(15): 1431. CrossRef
Signalling pathways associated with IL‐6 production and epithelial–mesenchymal transition induction in prostate epithelial cells stimulated with Trichomonas vaginalis
I. H. Han, J. H. Kim, S. S. Kim, M. H. Ahn, J. S. Ryu
Parasite Immunology.2016; 38(11): 678. CrossRef

14,738 View
178 Download
24 Web of Science
Crossref

Afatinib Reduces STAT6 Signaling of Host ARPE-19 Cells Infected with Toxoplasma gondii: Zhaoshou Yang, Hye-Jin Ahn, Young-Hoon Park, Ho-Woo Nam; Korean J Parasitol 2016;54(1):31-38.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.31

Abstract
PDF

Specific gene expressions of host cells by spontaneous STAT6 phosphorylation are major strategy for the survival of intracellular Toxoplasma gondii against parasiticidal events through STAT1 phosphorylation by infection provoked IFN-γ. We determined the effects of small molecules of tyrosine kinase inhibitors (TKIs) on the growth of T. gondii and on the relationship with STAT1 and STAT6 phosphorylation in ARPE-19 cells. We counted the number of T. gondii RH tachyzoites per parasitophorous vacuolar membrane (PVM) after treatment with TKIs at 12-hr intervals for 72 hr. The change of STAT6 phosphorylation was assessed via western blot and immunofluorescence assay. Among the tested TKIs, Afatinib (pan ErbB/EGFR inhibitor, 5 ?M) inhibited 98.0% of the growth of T. gondii, which was comparable to pyrimethamine (5 ?M) at 96.9% and followed by Erlotinib (ErbB1/EGFR inhibitor, 20 ?M) at 33.8% and Sunitinib (PDGFR or c-Kit inhibitor, 10 ?M) at 21.3%. In the early stage of the infection (2, 4, and 8 hr after T. gondii challenge), Afatinib inhibited the phosphorylation of STAT6 in western blot and immunofluorescence assay. Both JAK1 and JAK3, the upper hierarchical kinases of cytokine signaling, were strongly phosphorylated at 2 hr and then disappeared entirely after 4 hr. Some TKIs, especially the EGFR inhibitors, might play an important role in the inhibition of intracellular replication of T. gondii through the inhibition of the direct phosphorylation of STAT6 by T. gondii.

Citations

Citations to this article as recorded by

Small molecule kinase inhibitor altiratinib inhibits brain cyst forming bradyzoites of Toxoplasma gondii
Yeong Hoon Kim, Hye-Jin Ahn, Hwa Sun Kim, Ho-Woo Nam
Journal of Microbiology.2025; 63(2): e2409001. CrossRef
iTRAQ-Based Phosphoproteomic Analysis Exposes Molecular Changes in the Small Intestinal Epithelia of Cats after Toxoplasma gondii Infection
Bintao Zhai, Yu-Meng Meng, Shi-Chen Xie, Jun-Jie Peng, Yang Liu, Yanhua Qiu, Lu Wang, Jiyu Zhang, Jun-Jun He
Animals.2023; 13(22): 3537. CrossRef
Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors
Hye-Jung Kim, Hye-Jin Ahn, Hyeweon Kang, Jaehui Park, Seul gi Oh, Saehae Choi, Won-Kyu Lee, Ho-Woo Nam
The Korean Journal of Parasitology.2020; 58(3): 249. CrossRef
A Human Proteome Array Approach to Identifying Key Host Proteins Targeted by Toxoplasma Kinase ROP18
Zhaoshou Yang, Yongheng Hou, Taofang Hao, Hee-Sool Rho, Jun Wan, Yizhao Luan, Xin Gao, Jianping Yao, Aihua Pan, Zhi Xie, Jiang Qian, Wanqin Liao, Heng Zhu, Xingwang Zhou
Molecular & Cellular Proteomics.2017; 16(3): 469. CrossRef
Adverse Event Profile of Pyrimethamine-Based Therapy in Toxoplasmosis: A Systematic Review
Ruben R. Ben-Harari, Elizabeth Goodwin, Julio Casoy
Drugs in R&D.2017; 17(4): 523. CrossRef
Suppressors for Human Epidermal Growth Factor Receptor 2/4 (HER2/4): A New Family of Anti-Toxoplasmic Agents in ARPE-19 Cells
Yeong Hoon Kim, Lokraj Bhatt, Hye-Jin Ahn, Zhaoshou Yang, Won-Kyu Lee, Ho-Woo Nam
The Korean Journal of Parasitology.2017; 55(5): 491. CrossRef

10,883 View
103 Download
6 Web of Science
Crossref

Activation of MAPK Is Required for ROS Generation and Exocytosis in HMC-1 Cells Induced by Trichomonas vaginalis-Derived Secretory Products: Giimaa Narantsogt, Arim Min, Young Hee Nam, Young Ah Lee, Kyeong Ah Kim, Gurbadam Agvaandaram, Temuulen Dorjsuren, Jamel El-Benna, Myeong Heon Shin; Korean J Parasitol 2015;53(5):597-603.
Published online October 29, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.5.597

Abstract
PDF

Trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. Mast cells have been reported to be predominant in vaginal smears and vaginal walls of patients infected with T. vaginalis. Mitogen-activated protein kinase (MAPK), activated by various stimuli, have been shown to regulate the transcriptional activity of various cytokine genes in mast cells. In this study, we investigated whether MAPK is involved in ROS generation and exocytotic degranulation in HMC-1 cells induced by T. vaginalis-derived secretory products (TvSP). We found that TvSP induces the activation of MAPK and NADPH oxidase in HMC-1 cells. Stimulation with TvSP induced phosphorylation of MAPK and p47^phox in HMC-1 cells. Stimulation with TvSP also induced up-regulation of CD63, a marker for exocytosis, along the surfaces of human mast cells. Pretreatment with MAPK inhibitors strongly inhibited TvSP-induced ROS generation and exocytotic degranulation. Finally, our results suggest that TvSP induces intracellular ROS generation and exocytotic degranulation in HMC-1 via MAPK signaling.

Citations

Citations to this article as recorded by

Dynamin 2-mediated endocytosis of BLT1 is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2024; 62(3): 281. CrossRef
Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility
Zhenchao Zhang, Fakun Li, Yangyang Deng, Yuhua Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lihua Guo, Lixia Hao, Xuefang Mei
Acta Tropica.2023; 238: 106794. CrossRef
Latent Upregulation of Nlrp3, Nlrc4 and Aim2 Differentiates between Asymptomatic and Symptomatic Trichomonas vaginalis Infection
Sonal Yadav, Vivek Verma, Rakesh Singh Dhanda, Sumeeta Khurana, Manisha Yadav
Immunological Investigations.2022; 51(5): 1127. CrossRef
Trichomonas vaginalis Induces SiHa Cell Apoptosis by NF-κB Inactivation via Reactive Oxygen Species
Juan-Hua Quan, Byung-Hun Kang, Jung-Bo Yang, Yun-Ee Rhee, Heung-Tae Noh, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee
BioMed Research International.2017; 2017: 1. CrossRef
SNAP23-Dependent Surface Translocation of Leukotriene B 4 (LTB 4 ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secrete
Arim Min, Young Ah Lee, Kyeong Ah Kim, Jamel El-Benna, Myeong Heon Shin, Judith A. Appleton
Infection and Immunity.2017;[Epub] CrossRef
Explore the variation of MMP3, JNK, p38 MAPKs, and autophagy at the early stage of osteoarthritis
Jie Shi, Changjie Zhang, Zhongjie Yi, Chunna Lan
IUBMB Life.2016; 68(4): 293. CrossRef

11,097 View
109 Download
8 Web of Science
Crossref

Brief Communications

High Genetic Variability of Schistosoma haematobium in Mali and Nigeria: Charles Ezeh, Mingbo Yin, Hongyan Li, Ting Zhang, Bin Xu, Moussa Sacko, Zheng Feng, Wei Hu; Korean J Parasitol 2015;53(1):129-134.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.129

Abstract
PDF

Schistosoma haematobium is one of the most prevalent parasitic flatworms, infecting over 112 million people in Africa. However, little is known about the genetic diversity of natural S. haematobium populations from the human host because of the inaccessible location of adult worms in the host. We used 4 microsatellite loci to genotype individually pooled S. haematobium eggs directly from each patient sampled at 4 endemic locations in Africa. We found that the average allele number of individuals from Mali was significantly higher than that from Nigeria. In addition, no significant difference in allelic composition was detected among the populations within Nigeria; however, the allelic composition was significantly different between Mali and Nigeria populations. This study demonstrated a high level of genetic variability of S. haematobium in the populations from Mali and Nigeria, the 2 major African endemic countries, suggesting that geographical population differentiation may occur in the regions.

Citations

Citations to this article as recorded by

Evidence of high genetic diversity among parasite populations in a schistosomiasis hotspot
Yvonne Aryeetey Ashong, Emmanuel Odartei Armah, Jewelna Akorli, Frank Twum Aboagye, Isaac Owusu-Frimpong, Linda Batsa Debrah, Rhoda Lims Diyie, Samuel Armoo, Alexander Yaw Debrah, Mike Yaw Osei-Atweneboana, Clinton J. Jones, Marcello Otake Sato
Microbiology Spectrum.2025;[Epub] CrossRef
Geographical Influence on Morphometric Variability of Genetically “Pure” Schistosoma haematobium Eggs from Sub-Saharan Migrants in Spain
Marta Reguera-Gómez, Maria Adela Valero, Patricio Artigas, Alejandra De Elías-Escribano, Maria Cecilia Fantozzi, Maria Pilar Luzón-García, Joaquín Salas-Coronas, Jérôme Boissier, Santiago Mas-Coma, Maria Dolores Bargues
Tropical Medicine and Infectious Disease.2023; 8(3): 144. CrossRef
Genetic Diversity of Schistosoma haematobium in Sub-Saharan Africa: A Systematic Review
Rabecca Tembo, Panji Nkhoma, Mildred Zulu, Florence Mwaba, John Yabe, Hikabasa Halwiindi, Moses Kamwela, King S Nalubamba, Chummy S Sikasunge, Andrew M Phri
University of Zambia Journal of Agricultural and Biomedical Sciences.2022;[Epub] CrossRef
Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases
Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Detection of Schistosoma DNA in genital specimens and urine: A comparison between five female African study populations originating from S. haematobium and/or S. mansoni endemic areas
P. Pillay, J.A. Downs, J.M. Changalucha, E.A.T. Brienen, C.E. Ramarokoto, P.D.C. Leutscher, B.J. Vennervald, M. Taylor, E.F. Kjetland, L. Van Lieshout
Acta Tropica.2020; 204: 105363. CrossRef
RETRACTED: Diversity and Compatibility of Human Schistosomes and Their Intermediate Snail Hosts
Benjamin Sanogo, Dongjuan Yuan, Xin Zeng, Yanhua Zhang, Zhongdao Wu
Trends in Parasitology.2018; 34(6): 493. CrossRef
Geographic strain differentiation of Schistosoma japonicum in the Philippines using microsatellite markers
Kharleezelle J. Moendeg, Jose Ma M. Angeles, Ryo Nakao, Lydia R. Leonardo, Ian Kendrich C. Fontanilla, Yasuyuki Goto, Masashi Kirinoki, Elena A. Villacorte, Pilarita T. Rivera, Noboru Inoue, Yuichi Chigusa, Shin-ichiro Kawazu, Aaron R. Jex
PLOS Neglected Tropical Diseases.2017; 11(7): e0005749. CrossRef
Genetic Diversity of <i>Schistosoma haematobium</i> Eggs Isolated from Human Urine in Sudan
Juan-Hua Quan, In-Wook Choi, Hassan Ahmed Hassan Ahmed Ismail, Abdoelohab Saed Mohamed, Hoo-Gn Jeong, Jin-Su Lee, Sung-Tae Hong, Tai-Soon Yong, Guang-Ho Cha, Young-Ha Lee
The Korean Journal of Parasitology.2015; 53(3): 271. CrossRef

12,418 View
128 Download
9 Web of Science
Crossref

Chronic Opisthorchis viverrini Infection and Associated Hepatobiliary Disease Is Associated with Iron Loaded M2-like Macrophages: Moses T. Bility, Banchob Sripa; Korean J Parasitol 2014;52(6):695-699.
Published online December 23, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.6.695

Abstract
PDF

Chronic Opisthorchis viverrini-induced hepatobiliary disease is associated with significant leukocyte infiltration, including activated macrophages; however, the polarization of infiltrating macrophages remains to be fully characterized. In this study, we characterized macrophage polarization and phenotype in chronic O. viverrini-induced hepatobiliary disease in humans and hamsters using gene expression and histochemical analysis. Chronic O. viverrini infection and associated hepatobiliary diseases were associated with iron loaded M2-like macrophages in both humans and hamsters. This study provides suggestive evidence that iron loaded M2-like macrophages promote hepatobiliary disease in chronic O. viverrini infection.

Citations

Citations to this article as recorded by

Acute to chronic liver macrophage response in Syrian hamsters infected with the liver fluke Opisthorchis felineus
Anna Kovner, Yaroslav Kapushchak, Ekaterina Medvedeva, Oxana Zaparina, Maria Pakharukova
Acta Tropica.2026; 278: 108098. CrossRef
Liver Fluke-Derived Molecules Accelerate Skin Repair Processes in a Mouse Model of Type 2 Diabetes Mellitus
Anna Kovner, Yaroslav Kapushchak, Oxana Zaparina, Dmitry Ponomarev, Maria Pakharukova
International Journal of Molecular Sciences.2024; 25(22): 12002. CrossRef
Exploring the role of macrophages in determining the pathogenesis of liver fluke infection
Susel Loli Quinteros, Bronwyn O'Brien, Sheila Donnelly
Parasitology.2022; 149(10): 1364. CrossRef
Wound healing approach based on excretory-secretory product and lysate of liver flukes
Anna V. Kovner, Alena A. Tarasenko, Oxana Zaparina, Olga V. Tikhonova, Maria Y. Pakharukova, Viatcheslav A. Mordvinov
Scientific Reports.2022;[Epub] CrossRef
The pathogenic potential of the combined action of chronic Opisthorchis felineus infection and repeated social defeat stress in C57BL/6 mice
Damira Avgustinovich, Anna Kovner, Elena Kashina, Natalia Shatskaya, Galina Vishnivetskaya, Natalia Bondar, Maria Lvova
International Journal for Parasitology.2021; 51(5): 353. CrossRef
High macrophage activities are associated with advanced periductal fibrosis in chronic Opisthorchis viverrini infection
Kanin Salao, Krongkarn Watakulsin, Eimorn Mairiang, Sutas Suttiprapa, Sirikachorn Tangkawattana, Steven W. Edwards, Banchob Sripa
Parasite Immunology.2019;[Epub] CrossRef
Chronic hepatitis C infection–induced liver fibrogenesis is associated with M2 macrophage activation
Moses T. Bility, Kouki Nio, Feng Li, David R. McGivern, Stanley M. Lemon, Eoin R. Feeney, Raymond T. Chung, Lishan Su
Scientific Reports.2016;[Epub] CrossRef

9,213 View
79 Download
9 Web of Science
Crossref

Original Article

Prevalence of Cryptosporidium-Associated Diarrhea in a High Altitude-Community of Saudi Arabia Detected by Conventional and Molecular Methods: Yousry Hawash, Laila Sh. Dorgham, Ayman S. Al-Hazmi, Mohammed S. Al-Ghamdi; Korean J Parasitol 2014;52(5):479-485.
Published online October 22, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.5.479

Abstract
PDF

Cryptosporidium diarrhea represents a relevant clinical problem in developing countries. In Al-Taif, a city of Saudi Arabia that lies at an altitude of an around 2 km above the sea level, Cryptosporidium infection seems to be undiagnosed in nearly all clinical laboratories. Furthermore, nothing was published regarding Cryptosporidium-associated diarrhea in this area. The
objective
s of this research were to (1) determine the Cryptosporidium prevalence among patients with diarrhea and (2) to estimate the performances of 3 different diagnostic methods. Total 180 diarrheal fecal samples, 1 sample per patient, were collected between January and August 2013. Samples were screened for Cryptosporidium with modified Zeihl Neelsen (ZN) microscopy, RIDA® Quick lateral flow (LF) immunotest, and a previously published PCR. The Cryptosporidium prevalence rate was 9.4% (17/180), 10% (18/180), and 11.6% (21/180) by microscopy, LF, and PCR test, respectively. Infection was significantly (P=0.004) predominant among children <5 years (22%) followed by children 5-9 years (11.1%). Although infection was higher in males than in females (16.2% males and 8.5% females), the difference was not statistically significant (P=0.11). Compared to PCR, the sensitivity of microscopy and the LF test were 80.9%, 85.7%, respectively. To conclude, high Cryptosporidium-associated diarrhea was found in this area especially in children ≤9 years. The PCR test showed the best performance followed by the LF test and ZN staining microscopy. The primary health care providers in Al-Taif need to be aware of and do testing for this protozoon, particularly for children seen with diarrhea.

Citations

Citations to this article as recorded by

Intestinal Parasitic Infections Among Pediatric Patients in a Metropolitan City of Bangladesh With Emphasis on Cryptosporidiosis
Nusrat Jahan Nipa, Nasima Aktar, Hasina M Hira, Farhana Akter, Dilshad Jahan, Salequl Islam, Ayukafangha Etando, Adnan Abdullah, Kona Chowdhury, Rahnuma Ahmad, Ahsanul Haq, Mainul Haque
Cureus.2022;[Epub] CrossRef
Cryptosporidium and Cryptosporidiosis: The Perspective from the Gulf Countries
Shahira A. Ahmed, Panagiotis Karanis
International Journal of Environmental Research and Public Health.2020; 17(18): 6824. CrossRef
Foodborne intestinal protozoan infection and associated factors among patients with watery diarrhea in Northern Ethiopia; a cross-sectional study
Birhane Berhe, Gessessew Bugssa, Sena Bayisa, Megbaru Alemu
Journal of Health, Population and Nutrition.2018;[Epub] CrossRef
Human cryptosporidiosis in Iran: a systematic review and meta-analysis
Reza Berahmat, Adel Spotin, Ehsan Ahmadpour, Mahmoud Mahami-Oskouei, Azim Rezamand, Nayyereh Aminisani, Morteza Ghojazadeh, Roghayeh Ghoyounchi, Tahereh Mikaeili-Galeh
Parasitology Research.2017; 116(4): 1111. CrossRef
Cryptosporidium and Giardia Infections in Children
Blandina T. Mmbaga, Eric R. Houpt
Pediatric Clinics of North America.2017; 64(4): 837. CrossRef
High Frequency of Enteric Protozoan, Viral, and Bacterial Potential Pathogens in Community-Acquired Acute Diarrheal Episodes: Evidence Based on Results of Luminex Gastrointestinal Pathogen Panel Assay
Yousry A. Hawash, Khadiga A. Ismail, Mazen Almehmadi
The Korean Journal of Parasitology.2017; 55(5): 513. CrossRef
Assessment of microscopic and molecular tools for the diagnosis and follow-up of cryptosporidiosis in patients at risk
Y. Le Govic, K. Guyot, G. Certad, A. Deschildre, R. Novo, C. Mary, B. Sendid, E. Viscogliosi, L. Favennec, E. Dei-Cas, E. Fréalle, E. Dutoit
European Journal of Clinical Microbiology & Infectious Diseases.2016; 35(1): 137. CrossRef
Genetic characterization of Cryptosporidium in animal and human isolates from Jordan
Nawal Hijjawi, Rami Mukbel, Rongchang Yang, Una Ryan
Veterinary Parasitology.2016; 228: 116. CrossRef

11,724 View
110 Download
13 Web of Science
Crossref

Brief Communication

Gefitinib Inhibits the Growth of Toxoplasma gondii in HeLa Cells: Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam; Korean J Parasitol 2014;52(4):439-441.
Published online August 29, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.4.439

Abstract
PDF

Toxoplasma gondii is the causative agent of toxoplasmosis with symptoms of congenital neurological and ocular diseases and acquired lymphadenitis, retinochoroiditis, and meningoencephalitis. Small molecules which block the activity of protein kinases were tested in in vitro culture of T. gondii to find new therapeutic drugs of safer and more effective than the combined administration of pyrimethamine and sulfadoxine that sometimes provoke lethal Stevens-Johnson syndrome. Among them, Gefitinib and Crizotinib inhibited intracellular growth of T. gondii in HeLa cells by counting the number of T. gondii per parasitophorous vacuolar membrane whereas Sunitinib did not. Gefitinib inhibited the growth of T. gondii in a dose-dependent manner over 5 ?M up to the tolerable concentration of HeLa cells and halted the division of the parasite immediately from the time point of treatment. Gefitinib inhibition suggests that tyrosine kinases of EGFR family or other homologous kinases of the parasite itself may be the target to cause the block of T. gondii growth.

Citations

Citations to this article as recorded by

Experimental and clinical tests of FDA-approved kinase inhibitors for the treatment of neurological disorders (update 2024)
Hassan Aliashrafzadeh, Dewey Liu, Samantha De Alba, Imad Akbar, Austin Lui, Jordan Vanleuven, Ryan Martin, Zhang Wang, Da Zhi Liu
Exploration of Drug Science.2025;[Epub] CrossRef
The antimicrobial activity of innate host-directed therapies: A systematic review
Tirosh Shapira, Matthew Christofferson, Yossef Av-Gay
International Journal of Antimicrobial Agents.2024; 63(5): 107138. CrossRef
Impact of Training Data, Ground Truth and Shape Variability in the Deep Learning-Based Semantic Segmentation of HeLa Cells Observed with Electron Microscopy
Cefa Karabağ, Mauricio Alberto Ortega-Ruíz, Constantino Carlos Reyes-Aldasoro
Journal of Imaging.2023; 9(3): 59. CrossRef
Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathway
Xue-song Zhang, Yong-juan Zhao, Yu Zhang, Tong Xu, Kai-ling Cui, Bu-ting Duan, Xiao-ling Lv, Li Zhang, Zhi-yong Xu, Rui Bai, Ming-xue Zheng
Poultry Science.2022; 101(10): 102075. CrossRef
FDA-Approved Kinase Inhibitors in Preclinical and Clinical Trials for Neurological Disorders
Austin Lui, Jordan Vanleuven, David Perekopskiy, Dewey Liu, Desiree Xu, Omar Alzayat, Taiseer Elgokhy, Timothy Do, Meghan Gann, Ryan Martin, Da-Zhi Liu
Pharmaceuticals.2022; 15(12): 1546. CrossRef
Clearing or subverting the enemy: Role of autophagy in protozoan infections
George Ghartey-Kwansah, Benjamin Aboagye, Frank Adu-Nti, Yeboah Kwaku Opoku, Emmanuel Kwasi Abu
Life Sciences.2020; 247: 117453. CrossRef
Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors
Hye-Jung Kim, Hye-Jin Ahn, Hyeweon Kang, Jaehui Park, Seul gi Oh, Saehae Choi, Won-Kyu Lee, Ho-Woo Nam
The Korean Journal of Parasitology.2020; 58(3): 249. CrossRef
Autophagy in the control and pathogenesis of parasitic infections
George Ghartey-Kwansah, Frank Adu-Nti, Benjamin Aboagye, Amandus Ankobil, Edward Eyipe Essuman, Yeboah Kwaku Opoku, Samuel Abokyi, Emmanuel Kwasi Abu, Johnson Nyarko Boampong
Cell & Bioscience.2020;[Epub] CrossRef
Interplay Between Toxoplasma gondii, Autophagy, and Autophagy Proteins
Carlos S. Subauste
Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
The Interplay of Host Autophagy and Eukaryotic Pathogens
Robert J. Evans, Varadharajan Sundaramurthy, Eva-Maria Frickel
Frontiers in Cell and Developmental Biology.2018;[Epub] CrossRef
A Systematic Review of In vitro and In vivo Activities of Anti-Toxoplasma Drugs and Compounds (2006–2016)
Mahbobeh Montazeri, Mehdi Sharif, Shahabeddin Sarvi, Saeed Mehrzadi, Ehsan Ahmadpour, Ahmad Daryani
Frontiers in Microbiology.2017;[Epub] CrossRef
The Toxoplasma Parasitophorous Vacuole: An Evolving Host–Parasite Frontier
Barbara Clough, Eva-Maria Frickel
Trends in Parasitology.2017; 33(6): 473. CrossRef
Activation of a Neospora caninum EGFR-Like Kinase Facilitates Intracellular Parasite Proliferation
Xiaoxia Jin, Guojiang Li, Xichen Zhang, Pengtao Gong, Yanhui Yu, Jianhua Li
Frontiers in Microbiology.2017;[Epub] CrossRef
Suppressors for Human Epidermal Growth Factor Receptor 2/4 (HER2/4): A New Family of Anti-Toxoplasmic Agents in ARPE-19 Cells
Yeong Hoon Kim, Lokraj Bhatt, Hye-Jin Ahn, Zhaoshou Yang, Won-Kyu Lee, Ho-Woo Nam
The Korean Journal of Parasitology.2017; 55(5): 491. CrossRef
Human toxoplasmosis–Searching for novel chemotherapeutics
Magdalena Antczak, Katarzyna Dzitko, Henryka Długońska
Biomedicine & Pharmacotherapy.2016; 82: 677. CrossRef
Clinically Available Medicines Demonstrating Anti-Toxoplasma Activity
Andrew J. Neville, Sydney J. Zach, Xiaofang Wang, Joshua J. Larson, Abigail K. Judge, Lisa A. Davis, Jonathan L. Vennerstrom, Paul H. Davis
Antimicrobial Agents and Chemotherapy.2015; 59(12): 7161. CrossRef

10,432 View
96 Download
15 Web of Science
Crossref

Original Articles

Lethal Effects of Helianthemum lippii (L.) on Acanthamoeba castellanii Cysts in Vitro: F.A. Badria, M.H. Hetta, Rania M. Sarhan, M.H. Ezz El-Din; Korean J Parasitol 2014;52(3):243-249.
Published online June 26, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.3.243

Abstract
PDF

Acanthamoeba spp. commonly cause Acanthamoeba keratitis which is typically associated with the wear of contact lenses. Therefore, finding an economic, efficient, and safe therapy of natural origin is of outmost importance. This study examined the in vitro lethal potential of ethyl acetate and methanol extracts of Helianthemum lippii (L.) (sun roses) against Acanthamoeba castellanii cysts isolated from patients with amoebic keratitis. Both extracts proved to be potent as regard to their lethal effects on A. castellanii cysts with comparable results to chlorhexidine. The ethyl acetate was more promising with cumulative lethality. It showed a highly significant lethal percentage along the duration of treatment. The analysis of the more potent ethyl acetate extract revealed the presence of 2.96 mg/100 g of total phenolics, 0.289 mg/100 ml of total flavonoids and 37 mg/100 mg of total tannins which highlighted their phytomedicinal role.

Citations

Citations to this article as recorded by

In vitro amoebicidal and antioxidant activity of Helianthemum ledifolium (L.) Mill. varieties
Ayşe Baldemir Kılıç, Ülkü Karaman, Sadi Yusufbeyoğlu, Emine Burcu Yeşilyurt, Ayşe Turan Buğatekin, Yunus Güral, Türkan Mutlu Yar
Biological Diversity and Conservation.2026; 19(1): 74. CrossRef
Effectiveness of phytoproducts against pathogenic free-living amoebae - A scoping and critical review paving the way toward plant-based pharmaceuticals
Beni Jequicene Mussengue Chaúque, Thaisla Cristiane Borella da Silva, Eduardo Brittes Rott, Felipe Brittes Rott, Ana Paula Marçal Copetti Leite, Guilherme Brittes Benitez, Neuana Fernando Neuana, José Roberto Goldim, Marilise Brittes Rott, Régis Adriel Za
Fitoterapia.2025; 182: 106404. CrossRef
Phytochemical Profiles and Biological Activities of Frankenia Species: A Review
Meyada Khaled, Rachid Ouache, Patrick Pale, Hassina Harkat
Molecules.2024; 29(5): 980. CrossRef
Phytochemical on-line screening and in silico study of Helianthemum confertum: antioxidant activity, DFT, MD simulation, ADME/T analysis, and xanthine oxidase binding
Yasmine Chemam, Samir Benayache, Abdeslem Bouzina, Eric Marchioni, Omar Sekiou, Houria Bentoumi, Minjie Zhao, Zihad Bouslama, Nour-Eddine Aouf, Fadila Benayache
RSC Advances.2024; 14(31): 22209. CrossRef
Medicinal, Pharmacological and Biochemical Progress on the Study of Genus Helianthemum: A Review
Soumia Mouffouk, Chaima Mouffouk, Sara Mouffouk, Hamada Haba
Current Chemical Biology.2023; 17(3): 147. CrossRef
Phytochemical-rich extracts of Helianthemum lippii possess antimicrobial, anticancer, and anti-biofilm activities
Fabiana Plescia, Fabio Venturella, Antonella D’Anneo, Valentina Catania, Maria Letizia Gargano, Giulia Polito, Domenico Schillaci, Antonio Palumbo Piccionello, Marianna Lauricella, Giuseppe Venturella, Demetrio Raffa
Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology.2022; 156(6): 1314. CrossRef
Natural Products for Targeting Acanthamoeba spp.
Yassmin Isse Wehelie, Aishath Leesha Nasih, Ayaz Anwar, Ruqaiyyah Siddiqui, Sutherland Maciver, Naveed Ahmed Khan
Anti-Infective Agents.2022;[Epub] CrossRef
Anti-Acanthamoeba castellanii activity of alkaloid-enriched extracts and lycorine from the Amaryllidaceae species
Maressa Dietrich Rosa, Jean Paulo de Andrade, Adriana Oliveira Costa, Raphael Conti, Jaume Bastida, Warley de Souza Borges, Cinthia Furst
Brazilian Journal of Pharmaceutical Sciences.2022;[Epub] CrossRef
In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts
Ghada Mohamed Kadry, Mousa A. M. Ismail, Nagwa Mostafa El-Sayed, Hanan S. El-Kholy, Dina M. Hamdy El-Akkad
Journal of Parasitic Diseases.2021; 45(1): 159. CrossRef
In vitro Propagation to Conserve the Local Endemic and Endangered Medicinal Plant Helianthemum germanicopolitanum Bornm.
Emine Kapdan, Mehmet Sezgin
Brazilian Archives of Biology and Technology.2021;[Epub] CrossRef
Chemical composition, in vitro antiparasitic, antimicrobial and antioxidant activities of Frankenia thymifolia Desf.
Imad Mennai, Mourad Hanfer, Chahrazed Esseid, Samir Benayache, Souad Ameddah, Ahmed Menad, Fadila Benayache
Natural Product Research.2020; 34(23): 3363. CrossRef
Characterization of ethyl acetate and n-butanol extracts of Cymbopogon schoenanthus and Helianthemum lippii and their effect on the smooth muscle of the rat distal colon
Nihed Djemam, Somia Lassed, Fatih Gül, Muhammed Altun, Marisa Monteiro, Daniela Menezes-Pinto, Samir Benayache, Fadila Benayache, Djamila Zama, Ibrahim Demirtas, Manuela Morato
Journal of Ethnopharmacology.2020; 252: 112613. CrossRef
Amoebicidal and Amoebistatic Effects of Artemisia argyi Methanolic Extracts on Acanthamoeba castellanii Trophozoites and Cysts
Onur Kolören, Zeynep Kolören, Zülal Atli Şekeroğlu, Melek Çolayvaz, Panagiotis Karanis
Acta Parasitologica.2019; 64(1): 63. CrossRef
Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities
Tooba Mahboob, Muhammad Nawaz, Tan Tian-Chye, Chandramathi Samudi, Christophe Wiart, Veeranoot Nissapatorn
Pathogens.2018; 7(3): 62. CrossRef
Computational study of some amoebicidal phytochemicals against heat shock protein of Naegleria fowleri
Zarrin Basharat, Shumaila Zaib, Azra Yasmin
Gene Reports.2017; 6: 158. CrossRef

11,797 View
103 Download
12 Web of Science
Crossref

Angiostrongylus cantonensis: Scanning Electron Microscopic Observations on the Cuticle of Moulting Larvae: Xin Zeng, Jie Wei, Juan Wang, Feng Wu, Feng Fung, Xiaoying Wu, Xi Sun, Huanqing Zheng, Zhiyue Lv, Zhongdao Wu; Korean J Parasitol 2013;51(6):633-636.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.633

Abstract
PDF

Angiostrongylus cantonensis is a parasitic nematode that needs to develop in different hosts in different larval stages. Freshwater snails, such as Pomacea canaliculata, are the intermediate host, and rats are the definitive host. Periodic shedding of the cuticle (moulting) is an important biological process for the survival and development of the parasite in the intermediate and definitive hosts. However, there are few studies on the cuticle alterations between different stages of this parasite. In this study, we observed the ultrastructural appearance and changes of the cuticle of the 2nd/3rd stage larvae (L2/L3) and the 3rd/4th stage larvae (L3/L4) using a scanning electron microscope. We also first divided L2/L3 into late L2 and early L3. The late L2 lacked alae, but possessed a pull-chain-like fissure. Irregular alignment of spherical particles on the cuticle were noted compared to the L3. Alae appeared in the early L3. The old cuticle turned into a thin film-like structure which adhered to the new cuticle, and spherical particles were seen regularly arranged on the surface of this structure. Regular rectangular cavities were found on the surface of L3/L4. The caudal structure of L3/L4 was much larger than that of L3, but caudal inflation, such as seen in L4, was not observed. These results are the first to reveal the ultrastructural changes of the cuticle of A. cantonensis before and after moulting of L2/L3 and L3/L4.

Citations

Citations to this article as recorded by

Water transmission potential of Angiostrongylus cantonensis: Larval viability and effectiveness of rainwater catchment sediment filters
Kathleen Howe, Lisa Kaluna, Alicia Lozano, Bruce Torres Fischer, Yaeko Tagami, Robert McHugh, Susan Jarvi, Matty Knight
PLOS ONE.2019; 14(4): e0209813. CrossRef
The genetic basis of adaptive evolution in parasitic environment from the Angiostrongylus cantonensis genome
Lian Xu, Meng Xu, Xi Sun, Junyang Xu, Xin Zeng, Dai Shan, Dongjuan Yuan, Ping He, Weiming He, Yulan Yang, Shiqi Luo, Jie Wei, Xiaoying Wu, Zhen Liu, Xiaomin Xu, Zhensheng Dong, Langui Song, Beibei Zhang, Zilong Yu, Lifu Wang, Chi Zhang, Xiaodong Fang, Qia
PLOS Neglected Tropical Diseases.2019; 13(11): e0007846. CrossRef
Molluscicidal activity and mechanism of toxicity of a novel salicylanilide ester derivative against Biomphalaria species
Ping He, Weisi Wang, Benjamin Sanogo, Xin Zeng, Xi Sun, Zhiyue Lv, Dongjuan Yuan, Liping Duan, Zhongdao Wu
Parasites & Vectors.2017;[Epub] CrossRef
Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen
JOEL BARRATT, DOUGLAS CHAN, INDY SANDARADURA, RICHARD MALIK, DEREK SPIELMAN, ROGAN LEE, DEBORAH MARRIOTT, JOHN HARKNESS, JOHN ELLIS, DAMIEN STARK
Parasitology.2016; 143(9): 1087. CrossRef

10,064 View
115 Download
Crossref

Brief Communication

The Dimension of Trichomonas vaginalis as Measured by Scanning Electron Microscopy: Sang-Hoon Cheon, Seung Ryong Kim, Hyun-Ouk Song, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2013;51(2):243-246.
Published online April 25, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.2.243

Abstract
PDF

It is known that physicochemical conditions (e.g., pH, temperature, and ionic strength) affect the size of trichomonads. In this study, the sizes of 4 isolates of Trichomonas vaginalis cultured for more than a year (called "old T") and 3 isolates freshly isolated from vaginitis cases (called "fresh T") were compared by scanning electron microscopy. Although the fresh T had shorter body length, body width, and flagellar length than old T, total length (about 26 ?m), including body length, flagella length, and axostyle length was almost the same in the 2 groups. A striking difference was observed between the axostyles of the 2 groups; the axostyle length of the fresh T (8.2 ?m) was more than twice as long as that of the old T (4.0 ?m). However, in several parasitology textbooks, the length of T. vaginalis is said to vary widely from 7 to 32 ?m, and its undulating membrane is said to extend about half way (53.5%) to the posterior end of the body. On the other hand, in our study, the undulating membrane was observed to extend more than 3/4 of the body length (72.1%) in old T, whereas in fresh T it could not be measured. Taken together, we suggest that T. vaginalis averages 26 (21-32) ?m in total length, with 9.5 (7.4-11.4) ?m of body length and 6.8 (5.3-7.7) ?m of width, and its undulating membrane extending 3/4 of its body length. Therefore, these findings may provide useful information for morphological characteristics of T. vaginalis.

Citations

Citations to this article as recorded by

In vitro co-culture model of Trichomonas vaginalis, Candida albicans, and Lactobacillus crispatus: a system for assessing antimicrobial activity and microorganism interactions in vaginitis
Fernanda Gomes Cardoso, Luisa Trindade dos Santos, Saulo Almeida Menezes, Graziela Vargas Rigo, Tiana Tasca
Frontiers in Parasitology.2025;[Epub] CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Neutrophil interactions with the sexually transmitted parasite Trichomonas vaginalis: implications for immunity and pathogenesis
Suhani B. Bhakta, Jose A. Moran, Frances Mercer
Open Biology.2020;[Epub] CrossRef
Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis
Frances Mercer, Shek Hang Ng, Taylor M. Brown, Grace Boatman, Patricia J. Johnson, Fred Hughson
PLOS Biology.2018; 16(2): e2003885. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef

12,195 View
87 Download
Crossref

Original Article

Entamoeba histolytica Induces Cell Death of HT29 Colonic Epithelial Cells via NOX1-Derived ROS: Kyeong Ah Kim, Ju Young Kim, Young Ah Lee, Arim Min, Young Yil Bahk, Myeong Heon Shin; Korean J Parasitol 2013;51(1):61-68.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.61

Abstract
PDF

Entamoeba histolytica, which causes amoebic colitis and occasionally liver abscess in humans, is able to induce host cell death. However, signaling mechanisms of colon cell death induced by E. histolytica are not fully elucidated. In this study, we investigated the signaling role of NOX in cell death of HT29 colonic epithelial cells induced by E. histolytica. Incubation of HT29 cells with amoebic trophozoites resulted in DNA fragmentation that is a hallmark of apoptotic cell death. In addition, E. histolytica generate intracellular reactive oxygen species (ROS) in a contact-dependent manner. Inhibition of intracellular ROS level with treatment with DPI, an inhibitor of NADPH oxidases (NOXs), decreased Entamoeba-induced ROS generation and cell death in HT29 cells. However, pan-caspase inhibitor did not affect E. histolytica-induced HT29 cell death. In HT29 cells, catalytic subunit NOX1 and regulatory subunit Rac1 for NOX1 activation were highly expressed. We next investigated whether NADPH oxidase 1 (NOX1)-derived ROS is closely associated with HT29 cell death induced by E. histolytica. Suppression of Rac1 by siRNA significantly inhibited Entamoeba-induced cell death. Moreover, knockdown of NOX1 by siRNA, effectively inhibited E. histolytica-triggered DNA fragmentation in HT29 cells. These results suggest that NOX1-derived ROS is required for apoptotic cell death in HT29 colon epithelial cells induced by E. histolytica.

Citations

Citations to this article as recorded by

Parasites as Modulators of Angiogenesis: Implications for Vascular Biology and Pathogenesis
Tonathiu Rodríguez, Víctor H. Salazar-Castañón, Luis I. Terrazas, Imelda Juárez-Avelar, Miriam Rodríguez-Sosa
Pathogens.2026; 15(4): 347. CrossRef
Involvement of NOX2-derived ROS in human hepatoma HepG2 cell death induced by Entamoeba histolytica
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2023; 61(4): 388. CrossRef
Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica
Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin
The Korean Journal of Parasitology.2022; 60(3): 155. CrossRef
The interaction betweenEntamoeba histolyticaand enterobacteria shed light on an ancient antibacterial response
Nancy Guillén
Cellular Microbiology.2019;[Epub] CrossRef
NOX4 activation is involved in ROS‐dependent Jurkat T‐cell death induced by Entamoeba histolytica
Young Ah Lee, Kyeong Ah Kim, Arim Min, Myeong Heon Shin
Parasite Immunology.2019;[Epub] CrossRef
O-deGlcNAcylation is required for Entamoeba histolytica-induced HepG2 cell death
Young Ah Lee, Arim Min, Myeong Heon Shin
Microbial Pathogenesis.2018; 123: 285. CrossRef
GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways
Ming‑Zhen Wang, Cheng‑Zhi Qiu, Wai‑Shi Yu, Yan‑Ta Guo, Chun‑Xiao Wang, Zhi‑Xiong Chen
Molecular Medicine Reports.2017;[Epub] CrossRef
Infection Strategies of Intestinal Parasite Pathogens and Host Cell Responses
Bruno M. Di Genova, Renata R. Tonelli
Frontiers in Microbiology.2016;[Epub] CrossRef
A whole-genome RNAi screen uncovers a novel role for human potassium channels in cell killing by the parasite Entamoeba histolytica
Chelsea Marie, Hans P. Verkerke, Dan Theodorescu, William A. Petri
Scientific Reports.2015;[Epub] CrossRef
Degradation of the Transcription Factors NF-κB, STAT3, and STAT5 Is Involved in Entamoeba histolytica-Induced Cell Death in Caco-2 Colonic Epithelial Cells
Kyeong Ah Kim, Arim Min, Young Ah Lee, Myeong Heon Shin
The Korean Journal of Parasitology.2014; 52(5): 459. CrossRef
Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice
Rima Ramonaite
World Journal of Gastroenterology.2014; 20(35): 12533. CrossRef
Small GTPases of the Ras superfamily regulate intestinal epithelial homeostasis and barrier function via common and unique mechanisms
Alí Francisco Citalán-Madrid, Alexander García-Ponce, Hilda Vargas-Robles, Abigail Betanzos, Michael Schnoor
Tissue Barriers.2013; 1(5): e26938. CrossRef
The EhCPADH112 Complex of Entamoeba histolytica Interacts with Tight Junction Proteins Occludin and Claudin-1 to Produce Epithelial Damage
Abigail Betanzos, Rosario Javier-Reyna, Guillermina García-Rivera, Cecilia Bañuelos, Lorenza González-Mariscal, Michael Schnoor, Esther Orozco, Johanna M. Brandner
PLoS ONE.2013; 8(6): e65100. CrossRef

11,620 View
103 Download
Crossref

Brief Communications

Electron Microscopy of the Separated Outer Tegument of the Sparganum and Its Antigenicity: Hyun-Jong Yang; Korean J Parasitol 2012;50(2):181-183.
Published online May 24, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.2.181

Abstract
PDF

The author reported previously on separation of the outer tegument of the spargana (plerocercoids of Spirometra mansoni) using high concentration of urea solution. To determine which layer of the tegument is separated by this method, an electron microscopic analysis has been processed in this study. It was confirmed that the basement layer of the tegument is separated from the parenchyme of the sparganum. In addition, the antigenicity of the separated outer tegument against the human sparganosis patient sera was evaluated. Numerous antigenic proteins, including 16 and 55 kDa proteins, were noticed in the separated tegument; however, there were no diagnostic 31/36 kDa molecules in this tegument. The molecules reactive with the patient sera in the tegument are to be characterized in future studies.

7,702 View
60 Download

PCR for Diagnosis of Male Trichomonas vaginalis Infection with Chronic Prostatitis and Urethritis: Jong Jin Lee, Hong Sang Moon, Tchun Yong Lee, Hwan Sik Hwang, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2012;50(2):157-159.
Published online May 24, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.2.157

Abstract
PDF

The aim of this study was to assess the usefulness of PCR for diagnosis of Trichomonas vaginalis infection among male patients with chronic recurrent prostatitis and urethritis. Between June 2001 and December 2003, a total of 33 patients visited the Department of Urology, Hanyang University Guri Hospital and were examined for T. vaginalis infection by PCR and culture in TYM medium. For the PCR, we used primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Voided bladder urine (VB1 and VB3) was sampled from 33 men with symptoms of lower urinary tract infection (urethral charge, residual urine sensation, and frequency). Culture failed to detect any T. vaginalis infection whereas PCR identified 7 cases of trichomoniasis (21.2%). Five of the 7 cases had been diagnosed with prostatitis and 2 with urethritis. PCR for the 5 prostatitis cases yielded a positive 330 bp band from bothVB1 and VB3, whereas positive results were only obtained from VB1 for the 2 urethritis patients. We showed that the PCR method could detect T. vaginalis when there was only 1 T. vaginalis cell per PCR mixture. Our results strongly support the usefulness of PCR on urine samples for detecting T. vaginalis in chronic prostatitis and urethritis patients.

Citations

Citations to this article as recorded by

Trikomoniyaz tanısında farklı laboratuvar yöntemlerinin kullanılması ve Trikomoniyaz hastalarında miRNA profilinin belirlenmesi
Hasan Turgut, Fadime Eroğlu
Dicle Tıp Dergisi.2024; 51(1): 89. CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene
Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Detection of Trichomonas vaginalis Infection in Chronic Prostatitis/Chronic Pelvic Pain Syndrome Patients by Rapid Immunochromatographic Test
Po-Chih Chang, Ming-Li Hsieh, Shih-Tsung Huang, Hsin-Chieh Huang, Yu-Chao Hsu, Ching-Wei Huang, Wei-Feng Ding, Yu Chen
Polish Journal of Microbiology.2022; 71(3): 301. CrossRef
Increased diagnostic yield of routine multiplex PCR compared to clinician requested testing for detection of Trichomonas vaginalis
Brooke Webb, Andrea Crampton, Michelle J. Francis, John Hamblin, Tony M. Korman, Maryza Graham
Pathology.2021; 53(2): 257. CrossRef
Signaling Role of Adipocyte Leptin in Prostate Cell Proliferation Induced by Trichomonas vaginalis
Jung-Hyun Kim, Ik-Hwan Han, Su-Jin Shin, Sung-Yul Park, Hyo-Yeoung Chung, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(3): 235. CrossRef
Cáncer y Microbiota
Francisco Arvelo, Felipe Sojo, Carlos Cotte
Investigación Clínica.2021; 62(4): 407. CrossRef
Proliferation of Mouse Prostate Cancer Cells Inflamed by Trichomonas vaginalis
Sang-Su Kim, Kyu-Shik Kim, Ik-Hwan Han, Yeseul Kim, Seong Sik Bang, Jung-Hyun Kim, Yong-Suk Kim, Soo-Yeon Choi, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(6): 547. CrossRef
IL-6 produced by prostate epithelial cells stimulated with Trichomonas vaginalis promotes proliferation of prostate cancer cells by inducing M2 polarization of THP-1-derived macrophages
Ik-Hwan Han, Hyun-Ouk Song, Jae-Sook Ryu, Michael H. Hsieh
PLOS Neglected Tropical Diseases.2020; 14(3): e0008126. CrossRef
Advancing Prevention of STIs by Developing Specific Serodiagnostic Targets: Trichomonas vginalis as a Model
John F. Alderete
International Journal of Environmental Research and Public Health.2020; 17(16): 5783. CrossRef
Experimental rat prostatitis caused by Trichomonas vaginalis infection
Ki‐Seok Jang, Ik‐Hwan Han, Seung‐Ju Lee, Jin Yoo, Ye‐Seul Kim, Seobo Sim, Jae‐Sook Ryu
The Prostate.2019; 79(4): 379. CrossRef
Comparison of Seropositivity to Trichomonas vaginalis between Men with Prostatic Tumor and Normal Men
Jung-Hyun Kim, Hong-Sang Moon, Kyu-Shik Kim, Hwan-Sik Hwang, Jae-Sook Ryu, Sung-Yul Park
The Korean Journal of Parasitology.2019; 57(1): 21. CrossRef
Comparison of Two PCR Assays for Trichomonas vaginalis
Chang-Suk Noh, Sang-Su Kim, Sung-Yul Park, Hong-Sang Moon, Yeonchul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2019; 57(1): 27. CrossRef
Inflammatory mediators of prostate epithelial cells stimulated with Trichomonas vaginalis promote proliferative and invasive properties of prostate cancer cells
Ik‐Hwan Han, Jung‐Hyun Kim, Ki‐Seok Jang, Jae‐Sook Ryu
The Prostate.2019; 79(10): 1133. CrossRef
Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens
Collins Adjei, Richard Boateng, Albert Dompreh, Bismark Okyere, Eddie-Williams Owiredu
Tropical Medicine and Health.2019;[Epub] CrossRef
The Microbiome of Prostate Fluid Is Associated With Prostate Cancer
Xiaowei Ma, Chenfei Chi, Liancheng Fan, Baijun Dong, Xiaoguang Shao, Shaowei Xie, Min Li, Wei Xue
Frontiers in Microbiology.2019;[Epub] CrossRef
Proliferation of prostate epithelia induced by IL‐6 from stroma reacted with Trichomonas vaginalis
J.‐H. Kim, I.‐H. Han, Y.‐S. Kim, C.‐S. Noh, J.‐S. Ryu
Parasite Immunology.2018;[Epub] CrossRef
Trichomonas vaginalis infection and the diagnostic significance of detection tests among Ghanaian outpatients
Richard Harry Asmah, Rita Ofosuaa Agyeman, Noah Obeng-Nkrumah, Harriet Blankson, Georgina Awuah-Mensah, Momodou Cham, Listowell Asare, Patrick Ferdinand Ayeh-Kumi
BMC Women's Health.2018;[Epub] CrossRef
Epitopes within recombinant α-actinin protein is serodiagnostic target for Trichomonas vaginalis sexually transmitted infections
J.F. Alderete
Heliyon.2017; 3(1): e00237. CrossRef
Interaction between Trichomonas vaginalis and the Prostate Epithelium
Jung-Hyun Kim, Ik-Hwan Han, Sang-Su Kim, Soon-Jung Park, Duk-Young Min, Myoung-Hee Ahn, Jae-Sook Ryu
The Korean Journal of Parasitology.2017; 55(2): 213. CrossRef
Trichomonas vaginalis induces IL‐1β production in a human prostate epithelial cell line by activating the NLRP3 inflammasome via reactive oxygen species and potassium ion efflux
Na‐Yeong Gu, Jung‐Hyun Kim, Ik‐Hwan Han, Su‐Jeong Im, Min‐Young Seo, Yong‐Hoon Chung, Jae‐Sook Ryu
The Prostate.2016; 76(10): 885. CrossRef
Inflammatory response of a prostate stromal cell line induced by Trichomonas vaginalis
S. J. Im, I. H. Han, J. H. Kim, N. Y. Gu, M. Y. Seo, Y. H. Chung, J. S. Ryu
Parasite Immunology.2016; 38(4): 218. CrossRef
Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea
Seung-Ryong Kim, Jung-Hyun Kim, Na-Yeong Gu, Yong-Suk Kim, Yeon-Chul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2016; 54(2): 187. CrossRef
Inflammatory Responses in a Benign Prostatic Hyperplasia Epithelial Cell Line (BPH-1) Infected with Trichomonas vaginalis
Sang-Su Kim, Jung-Hyun Kim, Ik-Hwan Han, Myoung-Hee Ahn, Jae-Sook Ryu
The Korean Journal of Parasitology.2016; 54(2): 123. CrossRef
Proliferation of Prostate Stromal Cell Induced by Benign Prostatic Hyperplasia Epithelial Cell Stimulated WithTrichomonas vaginalisvia Crosstalk With Mast Cell
Jung-Hyun Kim, Sang-Su Kim, Ik-Hwan Han, Seobo Sim, Myoung-Hee Ahn, Jae-Sook Ryu
The Prostate.2016; 76(15): 1431. CrossRef
Detection of Trichomonas vaginalis in prostate tissue and serostatus in patients with asymptomatic benign prostatic hyperplasia
Jamshaid Iqbal, Jumanah Al-Rashed, Elijah O. Kehinde
BMC Infectious Diseases.2016;[Epub] CrossRef
Signalling pathways associated with IL‐6 production and epithelial–mesenchymal transition induction in prostate epithelial cells stimulated with Trichomonas vaginalis
I. H. Han, J. H. Kim, S. S. Kim, M. H. Ahn, J. S. Ryu
Parasite Immunology.2016; 38(11): 678. CrossRef
Epidemiology and laboratory characteristics ofTrichomonas vaginalisinfection in Croatian men with and without urethritis syndrome: a case–control study
Mario Sviben, Emilija Mlinarić Missoni, Tomislav Meštrović, Gordana Vojnović, Gordana Mlinarić Galinović
Sexually Transmitted Infections.2015; 91(5): 360. CrossRef
Trichomonas vaginalis: Clinical relevance, pathogenicity and diagnosis
Thomas Edwards, Patricia Burke, Helen Smalley, Glyn Hobbs
Critical Reviews in Microbiology.2014; : 1. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef
Prevalence of Trichomonas vaginalis by PCR in Men Attending a Primary Care Urology Clinic in South Korea
Jun-Hyeok Seo, Hye-Won Yang, So-Young Joo, Su-Min Song, Yu-Ran Lee, Jae-Sook Ryu, Eun Sang Yoo, Won Kee Lee, Hyun-Hee Kong, Sang-Eun Lee, Won-Ja Lee, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2014; 52(5): 551. CrossRef
Inflammatory response of prostate epithelial cells to stimulation by Trichomonas vaginalis
Min‐Young Seo, Su‐Jeong Im, Na‐Young Gu, Jung‐Hyun Kim, Yong‐Hoon Chung, Myoung‐Hee Ahn, Jae‐Sook Ryu
The Prostate.2014; 74(4): 441. CrossRef
Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
Hyun-Ouk Song, Jae-Sook Ryu
The Korean Journal of Parasitology.2013; 51(4): 479. CrossRef
A Survey Study of Trichomonas Vaginalis Infection in Unmarried Male Referred to Farmanfarmaean Clinic
Zarrintaj Valadkhani, Nayrreh Hassan, Zohreh Aghighi
Scimetr.2013;[Epub] CrossRef