Parasites, Hosts and Diseases

"Sang-Mi Lee"

Original Articles

Monoclonal antibodies to recombinant Der p 2, a major house dust mite allergen: specificity, epitope analysis and development of two-site capture ELISA: Tai-Soon Yong, Sang-Mi Lee, Gab-Man Park, In-Yong Lee, Han-Il Ree, Kyung-Sup Kim, Sang-Hwan Oh, Jung-Won Park, Chein-Soo Hong; Korean J Parasitol 1999;37(3):163-169.
Published online September 20, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.3.163

Abstract
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House dust mite allergens have been well established as sensitizing agents that are important in the induction of allergic diseases. In order to analyze epitopes of the allergen and to develop a quantitative method of the allergen exposure, monoclonal antibodies against a recombinant Der p 2 (rDer p 2), one of the major allergens of Dermatophagoides pteronyssinus, were produced. Four monoclonal antibodies produced were species-specific and did not cross-react to the D. farinae crude extract. Two of the monoclonal antibodies were found to be IgG1 and the others were IgM. For the analysis of epitopes, a Der p 2 cDNA encoding 126 amino acids (aa) was dissected into three fragments with several overlapping peptides, A (aa residues 1-49), B (44-93), and C fragment (84-126). Three monoclonal antibodies showed reactivities to the recombinant B fragment and to the full-length rDer p 2, but one monoclonal antibody reacted only with the full-length rDer p 2. Two-site capture ELISA was developed using two different monoclonal antibodies for quantitating Der p 2 in house dust. The sensitivity limit was 4 ng/ml with rDer p 2 and 8 ?g/ml with the D. pteronyssinus crude extract. The result suggested that the assay using monoclonal antibodies against rDer p 2 could be useful for the environmental studies and for the standardization of mite allergen extracts.

Citations

Citations to this article as recorded by

Determination of immunogenic epitopes in major house dust mite allergen, Der p 2, via nanoallergens
Jenna Sjoerdsma, Franklin Mejia, Basar Bilgicer
Annals of Allergy, Asthma & Immunology.2022; 129(2): 231. CrossRef
Mapping Mimotopes for House Dust Mite Allergen Der f 7 Using a Specific Monoclonal Antibody
Jianglong Peng, Hao Yin, Ying Zhou, Haoyuan Jia, Yubao Cui
Protein & Peptide Letters.2019; 26(3): 184. CrossRef
Expression and purification of a major allergen, Pla a 1, from Platanus acerifolia pollen and the preparation of its monoclonal antibody
Wei-Wei Ni, Wen Huang, De-Qin Wu, Yan-Jun Zhou, Chun-Mei Ji, Meng-Da Cao, Miao Guo, Jin-Lu Sun, Ji-Fu Wei
Molecular Medicine Reports.2017; 16(3): 2887. CrossRef
Review on Ecology of House Dust Mites in Korea and Suggestion of a Standard Survey Method
Tai-Soon Yong, Kyoung-Yong Jeong
Pediatric Allergy and Respiratory Disease.2011; 21(1): 4. CrossRef
Monoclonal antibodies against recombinant Der f 3 reveal localization of Der f 3 in the gut and faecal pellets of Dermatophagoides farinae
Zheng-ke Zhan, Kun-mei Ji, Xiao-yu Liu, Zhi-gang Liu, Meng Li, Jia-jie Chen, Jia-na Li, Shi Qiu
Experimental and Applied Acarology.2010; 52(1): 63. CrossRef
Household Arthropod Allergens in Korea
Tai-Soon Yong, Kyoung Yong Jeong
The Korean Journal of Parasitology.2009; 47(Suppl): S143. CrossRef
Mimotopes identify conformational B-cell epitopes on the two major house dust mite allergens Der p 1 and Der p 2
Krisztina Szalai, Jan Fuhrmann, Tea Pavkov, Michael Scheidl, Julia Wallmann, Kira H. Brämswig, Susanne Vrtala, Otto Scheiner, Walter Keller, Jean-Marie Saint-Remy, Dirk Neumann, Isabella Pali-Schöll, Erika Jensen-Jarolim
Molecular Immunology.2008; 45(5): 1308. CrossRef
Monoclonal antibodies to recombinant Der f 2 and development of a two‐site ELISA sensitive to major Der f 2 isoallergen in Korea
K.Y. Jeong, H.S. Jin, S.H. Oh, C.‐S. Hong, I.‐Y. Lee, H.‐I. Ree, T.‐S. Yong
Allergy.2002; 57(1): 29. CrossRef
Monoclonal antibodies to recombinant Der f 2 and development of a two-site ELISA sensitive to major Der f 2 isoallergen in Korea
K.Y. Jeong, H.S. Jin, S.H. Oh, C.-S. Hong, I.-Y. Lee, H.-I. Ree, T.-S. Yong
Allergy.2002; 57(1): 29. CrossRef
Localization of Der f 2 in the gut and fecal pellets of Dermatophagoides farinae
K. Y. Jeong, I.‐Y. Lee, H.‐I. Ree, C.‐S. Hong, T.‐S. Yong
Allergy.2002; 57(8): 729. CrossRef
Localization of a major allergen, Der p 2, in the gut and faecal pellets of Dermatophagoides pteronyssinus
Park, Lee, Lee, Ree, Kim, Hong, Yong
Clinical & Experimental Allergy.2000; 30(9): 1293. CrossRef

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Immunodiagnosis of clonorchiasis using a recombinant antigen: Tai-Soon Yong, Hye-Jin Yang, Soon-Jung Park, Yu-Kyoung Kim, Du-Ho Lee, Sang-Mi Lee; Korean J Parasitol 1998;36(3):183-190.
Published online September 30, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.3.183

Abstract
PDF

A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a β-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis.

Citations

Citations to this article as recorded by

A point-of-care testing assay for clonorchiasis using a EuNPs-CsTR1 fluorescent probe-based immunoassay
Xiaoxiao Ma, Huiyuan Zhang, Yiming Fang, Jing Wang, Xingyang Wang, Chen Li, Xiaolei Liu, Mingyuan Liu, Bin Tang, Yi Liu, Javier Sotillo
PLOS Neglected Tropical Diseases.2024; 18(4): e0012107. CrossRef
Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
Pyo Yun Cho, Ji-Yun Lee, Tae Im Kim, Jin-Ho Song, Sung-Jong Hong, Won Gi Yoo, Takafumi Tsuboi, Kwon-Soo Ha, Jae-Wan Jung, Satoru Takeo, Eun-Taek Han, Banchob Sripa, Sung-Tae Hong, Jong-Yil Chai, Ho-Woo Nam, Jhang Ho Pak, Tong-Soo Kim, Krystyna Cwiklinski
PLOS Neglected Tropical Diseases.2020; 14(12): e0008998. CrossRef
Cs1, a Clonorchis sinensis-derived serodiagnostic antigen containing tandem repeats and a signal peptide
Na Cheng, Xue-Nian Xu, Yan Zhou, Yu-Ting Dong, Yi-Fang Bao, Bin Xu, Wei Hu, Zheng Feng, Maria Elena Bottazzi
PLOS Neglected Tropical Diseases.2018; 12(8): e0006683. CrossRef
Development of a polymerase chain reaction applicable to rapid and sensitive detection ofClonorchis sinensiseggs in human stool samples
Pyo Yun Cho, Byoung-Kuk Na, Kyung Mi Choi, Jin Su Kim, Shin-Hyeong Cho, Won-Ja Lee, Sung-Bin Lim, Seok Ho Cha, Yun-Kyu Park, Jhang Ho Pak, Hyeong-Woo Lee, Sung-Jong Hong, Tong-Soo Kim
Pathogens and Global Health.2013; 107(5): 253. CrossRef
Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis
Shunyu Li, Jung Guk Shin, Pyo Yun Cho, Tae Im Kim, Sung-Tae Hong, Sung-Jong Hong
Parasitology Research.2011; 108(5): 1295. CrossRef
Proteomics of foodborne trematodes
Rafael Toledo, M. Dolores Bernal, Antonio Marcilla
Journal of Proteomics.2011; 74(9): 1485. CrossRef
Recent Advances in Serodiagnosis for Clonorchiasis
Tae Im Kim, Sung-Jong Hong
Hanyang Medical Reviews.2010; 30(3): 232. CrossRef
Functional Genes and Proteins of Clonorchis sinensis
Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
The Korean Journal of Parasitology.2009; 47(Suppl): S59. CrossRef
Molecular cloning and phylogenetic analysis of Clonorchis sinensis elongation factor-1α
Tae Yun Kim, Pyo Yun Cho, Jong Won Na, Sung-Jong Hong
Parasitology Research.2007; 101(6): 1557. CrossRef
Clonorchis sinensis: Development and evaluation of a nested polymerase chain reaction (PCR) assay
Ammini Parvathi, H. Sanath Kumar, B. Kenchanna Prakasha, Jieyuan Lu, Xuenian Xu, Wei Hu, Zheng Feng, Indrani Karunasagar, Iddya Karunasagar
Experimental Parasitology.2007; 115(3): 291. CrossRef
Clonorchiasis: an update
H.-J. Rim
Journal of Helminthology.2005; 79(3): 269. CrossRef
Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA
Min-Ho Choi, Il Chan Park, Shunyu Li, Sung-Tae Hong
The Korean Journal of Parasitology.2003; 41(1): 35. CrossRef
Immunology and molecular biology of Opisthorchis viverrini infection
S. Wongratanacheewin, R.W. Sermswan, S. Sirisinha
Acta Tropica.2003; 88(3): 195. CrossRef
Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis
Young-Bae Chung, Mejeong Lee, Hyun-Jong Yang, Byung-Suk Chung, Shun-Yu Lee, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2002; 40(2): 83. CrossRef
Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis
Tae Yun Kim, Shin-Yong Kang, Il-Young Ahn, Seung-Yull Cho, Sung-Jong Hong
The Korean Journal of Parasitology.2001; 39(1): 57. CrossRef
Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection
Sung-Jong Hong, Tae Yun Kim, Kye-Yong Song, Woon-Mok Sohn, Shin-Yong Kang
The Korean Journal of Parasitology.2001; 39(4): 307. CrossRef
Cystatin Capture Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Clonorchiasis and Profile of Captured Antigenic Protein ofClonorchis sinensis
Tae Yun Kim, Shin-Yong Kang, Sun Hyo Park, Kom Sukontason, Kabkaew Sukontason, Sung-Jong Hong
Clinical Diagnostic Laboratory Immunology.2001; 8(6): 1076. CrossRef
Identification of a Clonorchis sinensis gene encoding a vitellaria antigenic protein containing repetitive sequences
Hye-Jin Yang, Soon-Jung Park, Kyung-il Im, Tai-Soon Yong
Molecular and Biochemical Parasitology.2000; 111(1): 213. CrossRef