Parasites, Hosts and Diseases

"Sera Kim"

Original Article

ELISA detection of vivax malaria with recombinant multiple stage-specific antigens and its application to survey of residents in endemic areas: Sera Kim, Hye-Jin Ahn, Tong-Soo Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(4):203-207.
Published online December 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.4.203

Abstract
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An ELISA was developed for the diagnosis of vivax malaria using multiple stage-specific recombinant antigens of Plasmodium vivax. The DNA from the whole blood of a malaria patient was used as template to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), merozoite surface protein (MSP-1), apical merozoite antigen (AMA-1), serine repeat antigen (SERA), and exported antigen (EXP-1). Each amplified DNA fragment was inserted into pQE30 plasmid to induce the expression of His-tagged protein in Escherichia coli (M15 strain) by IPTG. His-tagged proteins were purified by Ni-NTA metal-affinity chromatography and used as antigens for ELISA with patient sera that were confirmed previously by blood smear examinations. When applied to patient sera, 122 (80.3%) out of 152 vivax malaria cases reacted to at least one antigen, while no reactions were observed with 128 uninfected serum samples. We applied this ELISA to the screening of 3,262 civilian residents in endemic regions near the DMZ, which resulted in 236 positively detected (7.2%) cases. This method can be applied to serological diagnosis and mass screening in endemic regions, or can be used as a safety test for transfusion blood in endemic areas.

Citations

Citations to this article as recorded by

Using Serological Markers for the Surveillance of Plasmodium vivax Malaria: A Scoping Review
Lejla Kartal, Ivo Mueller, Rhea J. Longley
Pathogens.2023; 12(6): 791. CrossRef
Antibodies Against the Plasmodium vivax Apical Membrane Antigen 1 From the Belem Strain Share Common Epitopes Among Other Worldwide Variants
Ana Caroline Barbosa França, Kátia Sanches Françoso, Rodolfo Ferreira Marques, Gustavo H. G. Trossini, Renan A. Gomes, Marinete M. Póvoa, Maristela G. Cunha, Eduardo L. V. Silveira, Irene S. Soares
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Analyses of the expression, immunohistochemical properties and serodiagnostic potential of Schistosoma japonicum peroxiredoxin-4
Minh-Anh Dang-Trinh, Jose Ma. M. Angeles, Kharleezelle J. Moendeg, Adrian Miki C. Macalanda, Thu-Thuy Nguyen, Luna Higuchi, Shotaro Nakagun, Masashi Kirinoki, Yuichi Chigusa, Yasuyuki Goto, Shin-ichiro Kawazu
Parasites & Vectors.2020;[Epub] CrossRef
Development and optimization of cocktail-ELISA for a unified surveillance of zoonotic schistosomiasis in multiple host species
Kharleezelle J. Moendeg, Jose Ma. M. Angeles, Yasuyuki Goto, Lydia R. Leonardo, Masashi Kirinoki, Elena A. Villacorte, Pilarita T. Rivera, Noboru Inoue, Yuichi Chigusa, Shin-ichiro Kawazu
Parasitology Research.2015; 114(3): 1225. CrossRef
Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients
Ho-Woo Nam, Kyoung Ju Song, Hye Jin Ahn, Zhaoshou Yang, Chom-Kyu Chong, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim
The Korean Journal of Parasitology.2014; 52(2): 143. CrossRef
Immunological markers of Plasmodium vivaxexposure and immunity: a systematic review and meta-analysis
Julia C Cutts, Rosanna Powell, Paul A Agius, James G Beeson, Julie A Simpson, Freya J I Fowkes
BMC Medicine.2014;[Epub] CrossRef
A Recombinant Plasmodium vivax Apical Membrane Antigen-1 to Detect Human Infection in Iran
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The Korean Journal of Parasitology.2012; 50(1): 15. CrossRef
Isolation and Characterization of the MSP1 Genes from Plasmodium malariae and Plasmodium ovale
George J. Dawson, Suresh M. Desai, Larry Birkenmeyer, A. Scott Muerhoff
The American Journal of Tropical Medicine and Hygiene.2010; 82(6): 996. CrossRef
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A. Scott Muerhoff, Larry G. Birkenmeyer, Ruthie Coffey, Bruce J. Dille, John W. Barnwell, William E. Collins, Joann S. Sullivan, George J. Dawson, Suresh M. Desai
Clinical and Vaccine Immunology.2010; 17(10): 1631. CrossRef
A new ELISA kit which uses a combination of Plasmodium falciparum extract and recombinant Plasmodium vivax antigens as an alternative to IFAT for detection of malaria antibodies
Cecile Doderer, Aurelie Heschung, Phillippe Guntz, Jean-Pierre Cazenave, Yves Hansmann, Alexandre Senegas, Alexander W Pfaff, Tamer Abdelrahman, Ermanno Candolfi
Malaria Journal.2007;[Epub] CrossRef

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Brief Communication

ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera: Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(3):175-177.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.175

Abstract
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An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a 6 × His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals.

Citations

Citations to this article as recorded by

Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Roloff, Fábio Pereira Leivas Leite
Revista Brasileira de Parasitologia Veterinária.2015; 24(2): 148. CrossRef
Diagnostic Potential of Anti-rNcp-43 Polyclonal Antibodies for the Detection of Neospora caninum
Gizele Lima de Sá, Diene de Borba Pacheco, Leonardo Garcia Monte, Francine Alves Sinnott, Marina Amaral Xavier, Caroline Rizzi, Sibele Borsuk, Maria Elisabeth Aires Berne, Renato Andreotti, Cláudia Pinho Hartleben
Current Microbiology.2014; 68(4): 472. CrossRef
Expression ofNeospora caninumNcSRS2 surface protein inPichia pastorisand its application for serodiagnosis ofNeosporainfection
Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Rollof, Fábio Pereira Leivas Leite
Pathogens and Global Health.2013; 107(3): 116. CrossRef
Development of an indirect ELISA-NcSRS2 for detection of Neospora caninum antibodies in cattle
Sibele Borsuk, Renato Andreotti, Fábio Pereira Leivas Leite, Luciano da Silva Pinto, Simone Simionatto, Claudia Pinho Hartleben, Marcelo Goetze, Leandra Marla Oshiro, Maria de Fátima Cepa Matos, Maria Elisabeth Aires Berne
Veterinary Parasitology.2011; 177(1-2): 33. CrossRef
Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA
Jing Liu, Jinshu Yu, Ming Wang, Qun Liu, Wei Zhang, Chong Deng, Jun Ding
Veterinary Parasitology.2007; 143(3-4): 358. CrossRef
Epidemiology and Control of Neosporosis andNeospora caninum
J. P. Dubey, G. Schares, L. M. Ortega-Mora
Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef
Diagnosis of bovine neosporosis: Recent advances and perspectives
Luis Ortega-Mora, Aurora Fernández-García, Mercedes Gómez-Bautista
Acta Parasitologica.2006; 51(1): 1. CrossRef
Diagnosis of bovine neosporosis
J.P. Dubey, G. Schares
Veterinary Parasitology.2006; 140(1-2): 1. CrossRef
Comparison of proteome and antigenic proteome between two Neospora caninum isolates
Yong-Seung Shin, Gee-Wook Shin, Young-Rim Kim, Eun-Young Lee, Hyang-Hee Yang, K.J. Palaksha, Hee-Jeong Youn, Jae-Hoon Kim, Dae-Yong Kim, A.E. Marsh, J. Lakritz, Tae-Sung Jung
Veterinary Parasitology.2005; 134(1-2): 41. CrossRef
HPLC purification of recombinant NcGRA6 antigen improves enzyme-linked immunosorbent assay for serodiagnosis of bovine neosporosis
M.C. Jenkins, R. Fetterer, G. Schares, C. Björkman, W. Wapenaar, M. McAllister, J.P. Dubey
Veterinary Parasitology.2005; 131(3-4): 227. CrossRef
Identification of antigenic proteins from Neospora caninum recognized by bovine immunoglobulins M, E, A and G using immunoproteomics
Yong‐seung Shin, Eung‐goo Lee, Gee‐wook Shin, Young‐rim Kim, Eun‐young Lee, Jae‐hoon Kim, Hwan Jang, Laurel J. Gershwin, Dae‐yong Kim, Yong‐hwan Kim, Gon‐sup Kim, Myung‐deuk Suh, Tae‐sung Jung
PROTEOMICS.2004; 4(11): 3600. CrossRef
Development of competitive ELISA for neosporosis by employing immunoproteomics
Yong-seung Shin, Eung-goo Lee, Gee-wook Shin, Young-rim Kim, Eun-young Lee, Jae-hoon Kim, Hwan Jang, Dae-yong Kim, Yong-hwan Kim, Gon-sup Kim, Myung-deuk Suh, Tae-sung Jung
Clinical Proteomics.2004;[Epub] CrossRef

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Original Article

Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients: Hye-Jin Ahn, Sera Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(2):89-96.
Published online June 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.2.89

Abstract
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Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy. The protein was not released into the parasitophorous vacuole during or after invasion. The cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg621. The full length cDNA sequence was completed with 5'-RACE as 1,592 bp, which contained open reading frame of 942 bp. The deduced amino acid sequence of Tg621 consisted of a polypeptide of 313 amino acids, with significant homology to ribosomal P proteins (RPP) of other organisms especially high to those of apicomplexan species. The expressed and purified TgRPP was assayed in western blot with the sera of toxoplasmosis patients and normal sera, which resulted in the 74.0% of positive reactions in toxoplasmosis patients whereas 8.3% in normal group. Therefore, the antibody formation against TgRPP in toxoplasmosis patients was regarded as specific for T. gondii infection and suggested a potential autoantibody.

Citations

Citations to this article as recorded by

Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli
M. Serdal Sevinc, Veena Kumar, Makonnen Abebe, Susantha Mohottalage, Premkumari Kumarathasan, Renaud Vincent, Hari M. Vijay
Protein Expression and Purification.2009; 65(1): 8. CrossRef
Identification of ribosomal phosphoprotein P0 of Neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis
Houshuang Zhang, Eung-goo Lee, Min Liao, Muller K.A. Compaore, Guohong Zhang, Osamu Kawase, Kozo Fujisaki, Chihiro Sugimoto, Yoshifumi Nishikawa, Xuenan Xuan
Molecular and Biochemical Parasitology.2007; 153(2): 141. CrossRef
Enzyme-catalyzed amplified immunoassay for the detection of Toxoplasma gondii-specific IgG using Faradaic impedance spectroscopy, CV and QCM
Yanjun Ding, Hua Wang, Guoli Shen, Ruqin Yu
Analytical and Bioanalytical Chemistry.2005; 382(7): 1491. CrossRef
Host cell binding of GRA10, a novel, constitutively secreted dense granular protein from Toxoplasma gondii
Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam
Biochemical and Biophysical Research Communications.2005; 331(2): 614. CrossRef
The P Domain of the P0 Protein ofPlasmodium falciparumProtects against Challenge with Malaria Parasites
K. Rajeshwari, Kalpesh Patel, Savithri Nambeesan, Monika Mehta, Alfica Sehgal, Tirtha Chakraborty, Shobhona Sharma
Infection and Immunity.2004; 72(9): 5515. CrossRef