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"Shin-Yong Kang"

Case Report

A human infection of Echinostoma hortense in duodenal bulb diagnosed by endoscopy
Young-Doo Chang, Woon-Mok Sohn, Jae-Hwa Ryu, Shin-Yong Kang, Sung-Jong Hong
Korean J Parasitol 2005;43(2):57-60.
Published online June 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.2.57

As gastroduodenoscopy performed more frequently, case reports of human echinostomiasis are increasing in Korea. A Korean woman presented at a local clinic with complaints of abdominal pain and discomfort that had persisted for 2 weeks. Under gastroduodenoscopy, two motile flukes were found attached on the duodenal bulb, and retrieved with endoscopic forceps. She had history of eating raw frog meat. The two flukes were identified as Echinostoma hortense by egg morphology, 27 collar spines with 4 end-group spines, and surface ultrastructural characters. This report may prove frogs to be a source of human echinostome infections.

Citations

Citations to this article as recorded by  Crossref logo
  • Clonorchis sinensis and Echinostoma hortense detected by endoscopy and molecular characterization: two case reports and update on diagnosis
    Lijia Wen, Benhe Wang, Hui Zhang
    Frontiers in Medicine.2025;[Epub]     CrossRef
  • Rare Case of Echinostoma cinetorchis Infection, South Korea
    Sooji Hong, Hyejoo Shin, Yoon-Hee Lee, Sung-Jong Hong, So-Ri Kim, Youn-Kyoung Kim, Young-Jin Son, Jeong-Gil Song, Jong-Yil Chai, Bong-Kwang Jung
    Emerging Infectious Diseases.2024;[Epub]     CrossRef
  • Type of cercaria in freshwater snails at Tunggu Pampang Reservoir, Makassar City, Indonesia
    Arif Rahman Jabal, Dian Mutiasari, Hairil Akbar, M. Arfah, Marhani Marhani, Rini Rini, Nur Alam Sobak, Anggit Julianingsih Pisu, Agnes Immanuela Toemon, Arini Ratnasari
    Russian Journal of Infection and Immunity.2023; 12(4): 765.     CrossRef
  • Neglected food-borne trematodiases: echinostomiasis and gastrodiscoidiasis
    Rafael Toledo, María Álvarez-Izquierdo, J. Guillermo Esteban, Carla Muñoz-Antoli
    Parasitology.2022; 149(10): 1319.     CrossRef
  • Morphology and Molecular Identification of Echinostoma revolutum and Echinostoma macrorchis in Freshwater Snails and Experimental Hamsters in Upper Northern Thailand
    Preeyaporn Butboonchoo, Chalobol Wongsawad, Pheravut Wongsawad, Jong-Yil Chai
    The Korean Journal of Parasitology.2020; 58(5): 499.     CrossRef
  • Fasciola hepatica infection in children actively detected in a survey in rural areas of Mardan district, Khyber Pakhtunkhawa province, northern Pakistan
    Asma W. Qureshi, Aurang Zeb, Abu Mansoor, Azam Hayat, Santiago Mas-Coma
    Parasitology International.2019; 69: 39.     CrossRef
  • Infection Status of Isthmiophora hortensis Metacercariae in Dark Sleepers, Odontobutis Species, from Some Water Systems of the Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju
    The Korean Journal of Parasitology.2018; 56(6): 633.     CrossRef
  • New Definitive Hosts and Differential Body Indices of Isthmiophora hortensis (Digenea: Echinostomatidae)
    Woon-Mok Sohn, Byoung-Kuk Na, Sung-Shik Shin
    The Korean Journal of Parasitology.2017; 55(3): 287.     CrossRef
  • Echinostoma macrorchis (Digenea: Echinostomatidae): Metacercariae in Cipangopaludina chinensis malleata Snails and Adults from Experimental Rats in Korea
    Woon-Mok Sohn, Byoung-Kuk Na
    The Korean Journal of Parasitology.2017; 55(5): 541.     CrossRef
  • Epidemiological analysis of human fascioliasis in northeastern Punjab, Pakistan
    Asma W. Qureshi, Akhtar Tanveer, Santiago Mas-Coma
    Acta Tropica.2016; 156: 157.     CrossRef
  • An update on human echinostomiasis
    R. Toledo, J. G. Esteban
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 37.     CrossRef
  • A Case of Echinostoma cinetorchis (Trematoda: Echinostomatidae) Infection Diagnosed by Colonoscopy
    Woon Tae Jung, Kyeong Ju Lee, Hong Jun Kim, Tae Hyo Kim, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2014; 52(3): 287.     CrossRef
  • Echinostoma hortense Infection with Enteritis Diagnosed by Upper Gastrointestinal Endoscopy in a Dog
    Hiroki OKANISHI, Jun MATSUMOTO, Sadao NOGAMI, Yumiko KAGAWA, Toshihiro WATARI
    Journal of Veterinary Medical Science.2013; 75(7): 991.     CrossRef
  • Evaluation of the role of rats as reservoir hosts for fishborne zoonotic trematodes in two endemic northern Vietnam fish farms
    Nguyen Lan Anh Thi, Henry Madsen, Dao Thanh Ha, Eric Hoberg, Anders Dalsgaard, K. Darwin Murrell
    Parasitology Research.2012; 111(3): 1045.     CrossRef
  • Proteomics of foodborne trematodes
    Rafael Toledo, M. Dolores Bernal, Antonio Marcilla
    Journal of Proteomics.2011; 74(9): 1485.     CrossRef
  • Echinostoma hortense and Heterophyid Metacercariae Encysted in Yellowfin Goby, Acanthogobius flavimanus, from Shinan-gun and Muan-gun (Jeollanam-do), Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho
    The Korean Journal of Parasitology.2009; 47(3): 307.     CrossRef
  • Foodborne Intestinal Flukes in Southeast Asia
    Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • ECHINOSTOMA HORTENSE ASADA INFECTION IN THE DUODENUM: INCIDENTAL FINDINGS DURING ROUTINE GASTROINTESTINAL ENDOSCOPY
    Toshiaki Tanaka, Tomoari Kamada, Hideki Koga, Aki Tanaka, Noriaki Manabe, Jiro Hata, Hiroaki Kusunoki, Manabu Ishii, Motonori Sato, Kenichi Tarumi, Akiko Shiotani, Tetsuya Okino, Ken Haruma
    Digestive Endoscopy.2008; 20(2): 87.     CrossRef
  • Eosinophilia: Causes and pathobiology in persons with prior exposures in tropical areas with an emphasis on parasitic infections
    Yae-Jean Kim, Thomas B. Nutman
    Current Infectious Disease Reports.2006; 8(1): 43.     CrossRef
  • 9,689 View
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Original Articles

Ultrastructural localization of 28 kDa glutathione S-transferase in adult Clonorchis sinensis
Sung-Jong Hong, Jae-Ran Yu, Shin-Yong Kang
Korean J Parasitol 2002;40(4):173-176.
Published online December 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.4.173

Glutathione S-transferase (28GST) with molecular mass of 28 kDa is an anti-oxidant enzyme abundant in Clonorchis sinensis. In adult C. sinensis, 28GST was localized in tegumental syncytium, cytons, parenchyma, and sperm tails examined by immunoelectron microscopy. C. sinensis 28GST was earlier found to neutralize bio-reactive compounds and to be rich in eggs. Accordingly, it is suggested that 28GST plays important roles in phase II defense system and physiological roles in worm fecundity of C. sinensis.

Citations

Citations to this article as recorded by  Crossref logo
  • Heat-killed Propionibacterium acnes augment the protective effect of 28-kDa glutathione S-transferases antigen against Schistosoma mansoni infection
    Ho Yin Pekkle Lam, Ting-Hua Yang, Ting-Ruei Liang, Po-Ching Cheng, Kai-Chih Chang, Shih-Yi Peng
    Acta Tropica.2021; 222: 106033.     CrossRef
  • Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis
    Mikhail Pomaznoy, Sergey Tatkov, Alexey Katokhin, Dmitry Afonnikov, Vladimir Babenko, Dagmara Furman, Ilya Brusentsov, Pavel Belavin, Alexandr Najakshin, Sergey Guselnikov, Gennady Vasiliev, Anton Sivkov, Egor Prokhortchouk, Konstantin Skryabin, Viatchesl
    Experimental Parasitology.2013; 135(2): 297.     CrossRef
  • Identification and biochemical characterization of two novel peroxiredoxins in a liver fluke,Clonorchis sinensis
    Y.-A. BAE, S.-H. KIM, E.-G. LEE, W.-M. SOHN, Y. KONG
    Parasitology.2011; 138(9): 1143.     CrossRef
  • Progress on the transcriptomics of carcinogenic liver flukes of humans—Unique biological and biotechnological prospects
    Neil D. Young, Aaron R. Jex, Cinzia Cantacessi, Bronwyn E. Campbell, Thewarach Laha, Woon-Mok Sohn, Banchob Sripa, Alex Loukas, Paul J. Brindley, Robin B. Gasser
    Biotechnology Advances.2010; 28(6): 859.     CrossRef
  • Functional Genes and Proteins of Clonorchis sinensis
    Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
    The Korean Journal of Parasitology.2009; 47(Suppl): S59.     CrossRef
  • Xenobiotic metabolizing enzymes and metabolism of anthelminthics in helminths
    Viktor Cvilink, Jiri Lamka, Lenka Skálová
    Drug Metabolism Reviews.2009; 41(1): 8.     CrossRef
  • Opisthorchis viverrini: Gene expression profiling of carcinogenic adult liver fluke worms using 5′ SAGE
    Nopporn Chutiwitoonchai, Yan Shen, Huajun Zheng, Hui Xiong, Guoping Zhao, Kanokwan Imtawil, Pewpan M. Intapan, Sopit Wongkham, Chaisiri Wongkham
    Experimental Parasitology.2008; 120(4): 306.     CrossRef
  • Clonorchiasis: an update
    H.-J. Rim
    Journal of Helminthology.2005; 79(3): 269.     CrossRef
  • 7,486 View
  • 69 Download
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Excretory bladder: the source of cysteine proteases in Paragonimus westermani metacercariae
Hyun-Jong Yang, Young-Bae Chung, Shin-Yong Kang, Yoon Kong, Seung-Yull Cho
Korean J Parasitol 2002;40(2):89-92.
Published online June 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.2.89

The cysteine proteases of Paragonimus westermani metacercariae are involved in metacercarial excystment, host immune modulation, and possibly in tissue penetration. In order to clarify the origin of the enzymes, 28 and 27 kDa cysteine proteases in metacercarial excretory-secretory products were purified through the FPLC system using Mono Q column chromatography. The polyclonal antibodies to the enzymes were produced in BALB/c mice. Immunolocalization studies revealed that both cysteine proteases were distributed at the linings of excretory bladder and excretory concretions of the metacercariae. It was suggested that the excretory epithelium of P. westermani undertake the secretory function of metacercarial cysteine proteases, in addition to its role as a route for eliminating waste products.

Citations

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  • Early Cysteine Protease Activity in Excretory Bladder Triggers Metacercaria Excystment of Paragonimus westermani
    Y. B. Chung, T. S. Kim, H. J. Yang
    Journal of Parasitology.2005; 91(4): 953.     CrossRef
  • 7,669 View
  • 69 Download
  • Crossref
Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection
Sung-Jong Hong, Tae Yun Kim, Kye-Yong Song, Woon-Mok Sohn, Shin-Yong Kang
Korean J Parasitol 2001;39(4):307-312.
Published online December 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.4.307

In the course of Clonorchis sinensis infection, antigens presented to the hosts may be in a close relation to growth of the fluke. The antigenic proteins stimulating IgG antibody production were chronologically identified by immunoblot and localized by immunohistochemical staining. In the early stage of infection until 12 weeks post-infection (PI), antigens were proteins with molecular mass larger than 34 kDa which were derived from the tegument, testes and intrauterine eggs. After 20 weeks PI, antigens recognized were 29, 27 and 26 kDa proteins from the intestine, excretory bladder and reproductive organs. It is suggested that the tegumental proteins are the most potent antigens and the excretory-secretory proteins with middle molecular mass of 26-45 kDa contribute to the high level production of antibodies after 20 weeks of the C. sinensis infection.

Citations

Citations to this article as recorded by  Crossref logo
  • Development of a multi-functional multi-probe atomic force microscope system with optical beam deflection method
    Peng Li, Yongjian Shao, Ke Xu, Xiaohui Qiu
    Review of Scientific Instruments.2021;[Epub]     CrossRef
  • Identification of Myoferlin, a Potential Serodiagnostic Antigen of Clonorchiasis, via Immunoproteomic Analysis of Sera From Different Infection Periods and Excretory-Secretory Products of Clonorchis sinensis
    Xiao-Xiao Ma, Yang-Yuan Qiu, Zhi-Guang Chang, Jun-Feng Gao, Rui-Ruo Jiang, Chun-Lin Li, Chun-Ren Wang, Qiao-Cheng Chang
    Frontiers in Cellular and Infection Microbiology.2021;[Epub]     CrossRef
  • Mapping of the putative epitope domain of Clonorchis sinensis paramyosin (CsPmy) recognized by CsPmy-specific immunoglobulin G in sera of human clonorchiasis
    Jung-Mi Kang, Hye-Lim Ju, Jinyoung Lee, Tae Im Kim, Shin-Hyeong Cho, Tong-Soo Kim, Woon-Mok Sohn, Byoung-Kuk Na
    Molecular and Biochemical Parasitology.2015; 201(1): 66.     CrossRef
  • Coproantigen capture ELISA for detection of Clonorchis sinensis infection in experimentally infected rats
    S.M. Mazidur Rahman, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
    Parasitology International.2012; 61(1): 203.     CrossRef
  • Functional Genes and Proteins of Clonorchis sinensis
    Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
    The Korean Journal of Parasitology.2009; 47(Suppl): S59.     CrossRef
  • Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory‐secretory products of Clonorchis sinensis adult worms
    Jung‐Won Ju, Hyun‐Na Joo, Myoung‐Ro Lee, Shin‐Hyeong Cho, Hyeng‐Il Cheun, Jung‐Yeon Kim, Young‐Hee Lee, Kwang‐Jun Lee, Woon‐Mok Sohn, Dong‐Min Kim, Il‐Chul Kim, Byoung Chul Park, Tong‐Soo Kim
    PROTEOMICS.2009; 9(11): 3066.     CrossRef
  • A novel tegumental protein 31.8 kDa of Clonorchis sinensis: sequence analysis, expression, and immunolocalization
    Yan Huang, Zhenwen Zhou, Xuchu Hu, Quande Wei, Jin Xu, Zhongdao Wu, Xinbing Yu
    Parasitology Research.2007; 102(1): 77.     CrossRef
  • Organ-specific antigens of Clonorchis sinensis
    Shunyu Li, Byung-Suk Chung, Min-Ho Choi, Sung-Tae Hong
    The Korean Journal of Parasitology.2004; 42(4): 169.     CrossRef
  • Clinical and epidemiological data of patients with clonorchiasis
    Ke-Xia Wang, Rong-Bo Zhang, Yu-Bao Cui, Ye Tian, Ru Cai, Chao-Pin Li
    World Journal of Gastroenterology.2004; 10(3): 446.     CrossRef
  • Use of a recombinant Clonorchis sinensis pore-forming peptide, clonorin, for serological diagnosis of clonorchiasis
    Ji-Yun Lee, Tae Yun Kim, Xiao-Xian Gan, Shin-Yong Kang, Sung-Jong Hong
    Parasitology International.2003; 52(2): 175.     CrossRef
  • 8,133 View
  • 79 Download
  • Crossref
Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis
Tae Yun Kim, Shin-Yong Kang, Il-Young Ahn, Seung-Yull Cho, Sung-Jong Hong
Korean J Parasitol 2001;39(1):57-66.
Published online March 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.1.57

In the course of immunoscreening of Clonorchis sinensis cDNA library, a cDNA CsRP12 containing a tandem repeat was isolated. The cDNA CsRP12 encodes two putative peptides of open reading frames (ORFs) 1 and 2 (CsRP12-1 and -2). The repetitive region is composed of 15 repeats of 10 amino acids. Of the two putative peptides, CsRP12-1 was proline-rich and found to have homologues in several organisms. Recombinant proteins of the putative peptides were bacterially produced and purified by an affinity chromatography. Recombinant CsRP12-1 protein was recognized by sera of clonorchiasis patients and experimental rabbits, but recombinant CsRP12-2 was not. One of the putative peptide, CsRP12-1, is designated CsPRA, proline-rich antigen of C. sinensis. Both the C-termini of CsRP12-1 and -2 were bacterially produced and analysed to show no antigenicity. Recombinant CsPRA protein showed high sensitivity and specificity. In experimental rabbits, IgG antibodies to CsPRA was produced between 4 and 8 weeks after the infection and decreased thereafter over one year. These results indicate that CsPRA is equivalent to a natural protein and a useful antigenic protein for serodiagnosis of human clonorchiasis.

Citations

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  • A point-of-care testing assay for clonorchiasis using a EuNPs-CsTR1 fluorescent probe-based immunoassay
    Xiaoxiao Ma, Huiyuan Zhang, Yiming Fang, Jing Wang, Xingyang Wang, Chen Li, Xiaolei Liu, Mingyuan Liu, Bin Tang, Yi Liu, Javier Sotillo
    PLOS Neglected Tropical Diseases.2024; 18(4): e0012107.     CrossRef
  • Evaluation of Crude and Recombinant Antigens of Schistosoma japonicum for the Detection of Schistosoma mekongi Human Infection
    Jose Ma. M. Angeles, Atcharaphan Wanlop, Minh-Anh Dang-Trinh, Masashi Kirinoki, Shin-ichiro Kawazu, Aya Yajima
    Diagnostics.2023; 13(2): 184.     CrossRef
  • Combination of Biodata Mining and Computational Modelling in Identification and Characterization of ORF1ab Polyprotein of SARS-CoV-2 Isolated from Oronasopharynx of an Iranian Patient
    Reza Zolfaghari Emameh, Hassan Nosrati, Ramezan Ali Taheri
    Biological Procedures Online.2020;[Epub]     CrossRef
  • Cs1, a Clonorchis sinensis-derived serodiagnostic antigen containing tandem repeats and a signal peptide
    Na Cheng, Xue-Nian Xu, Yan Zhou, Yu-Ting Dong, Yi-Fang Bao, Bin Xu, Wei Hu, Zheng Feng, Maria Elena Bottazzi
    PLOS Neglected Tropical Diseases.2018; 12(8): e0006683.     CrossRef
  • Heat shock proteins 70 and 90 from Clonorchis sinensis induce Th1 response and stimulate antibody production
    Eun Joo Chung, Young-Il Jeong, Myoung-Ro Lee, Yu Jung Kim, Sang-Eun Lee, Shin-Hyeong Cho, Won-Ja Lee, Mi-Yeoun Park, Jung-Won Ju
    Parasites & Vectors.2017;[Epub]     CrossRef
  • Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target
    Yu-Jung Kim, Won Yoo, Myoung-Ro Lee, Jung-Mi Kang, Byoung-Kuk Na, Shin-Hyeong Cho, Mi-Yeoun Park, Jung-Won Ju
    International Journal of Molecular Sciences.2017; 18(3): 557.     CrossRef
  • Development of “-omics” research in Schistosoma spp. and -omics-based new diagnostic tools for schistosomiasis
    Shuqi Wang, Wei Hu
    Frontiers in Microbiology.2014;[Epub]     CrossRef
  • Analysis of an expressed sequence tag library from Dicrocoelium dentriticum
    A.M. Martínez-Ibeas, M.J. Perteguer, C. González-Lanza, T. Gárate, M.Y. Manga-González
    Experimental Parasitology.2013; 135(2): 287.     CrossRef
  • Genomics and molecular genetics of Clonorchis sinensis: Current status and perspectives
    Si-Yang Huang, Guang-Hui Zhao, Bao-Quan Fu, Min-Jun Xu, Chun-Ren Wang, Song-Ming Wu, Feng-Cai Zou, Xing-Quan Zhu
    Parasitology International.2012; 61(1): 71.     CrossRef
  • Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis
    Shunyu Li, Jung Guk Shin, Pyo Yun Cho, Tae Im Kim, Sung-Tae Hong, Sung-Jong Hong
    Parasitology Research.2011; 108(5): 1295.     CrossRef
  • Human Antibody Response to Thioredoxin Peroxidase-1 and Tandem Repeat Proteins as Immunodiagnostic Antigen Candidates for Schistosoma japonicum Infection
    Shin-ichiro Kawazu, Masashi Kirinoki, Pilarita Tongol-Rivera, Yasuyuki Goto, Lydia Leonardo, Elena Villacorte, Yuichi Chigusa, Noboru Inoue, Jose Ma. Angeles
    The American Journal of Tropical Medicine and Hygiene.2011; 85(4): 674.     CrossRef
  • Recent Advances in Serodiagnosis for Clonorchiasis
    Tae Im Kim, Sung-Jong Hong
    Hanyang Medical Reviews.2010; 30(3): 232.     CrossRef
  • Functional Genes and Proteins of Clonorchis sinensis
    Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
    The Korean Journal of Parasitology.2009; 47(Suppl): S59.     CrossRef
  • Molecular cloning and phylogenetic analysis of Clonorchis sinensis elongation factor-1α
    Tae Yun Kim, Pyo Yun Cho, Jong Won Na, Sung-Jong Hong
    Parasitology Research.2007; 101(6): 1557.     CrossRef
  • Clonorchiasis: an update
    H.-J. Rim
    Journal of Helminthology.2005; 79(3): 269.     CrossRef
  • Immunology and molecular biology of Opisthorchis viverrini infection
    S. Wongratanacheewin, R.W. Sermswan, S. Sirisinha
    Acta Tropica.2003; 88(3): 195.     CrossRef
  • Use of a recombinant Clonorchis sinensis pore-forming peptide, clonorin, for serological diagnosis of clonorchiasis
    Ji-Yun Lee, Tae Yun Kim, Xiao-Xian Gan, Shin-Yong Kang, Sung-Jong Hong
    Parasitology International.2003; 52(2): 175.     CrossRef
  • Analysis of the genes expressed in Clonorchis sinensis adults using the expressed sequence tag approach
    Ji-Sook Lee, Jongweon Lee, Soon-Jung Park, Tai-Soon Yong
    Parasitology Research.2003; 91(4): 283.     CrossRef
  • 9,663 View
  • 69 Download
  • Crossref

Brief Communication

Differential expression of the 27 kDa cathepsin L-like cysteine protease in developmental stages of Spirometra erinacei
Yoon Kong, Doo-Hee Yun, Seung-Yull Cho, Woon-Mok Sohn, Young-Bae Chung, Shin-Yong Kang
Korean J Parasitol 2000;38(3):195-199.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.195

The 27 kDa cathepsin L-like cysteine protease of Spirometra erinacei plerocercoid is known to play an important function in tissue penetration, nutrient uptake and immune modulation in human sparganosis. In the present study, the expression of this enzyme was examined at different developmental stages of S. erinacei including immature egg, coracidium, plerocercoid in tadpole and rat, and adult. Proteolytic activity against carboxybenzoyl-phenylalanyl-arginyl-7-amino-4-methylcoumarin was detected in the extracts of coracidia and plerocercoid while no activity was observed in those of immature egg and adult. The specific activity in coracidial extracts was lower than that in the plerocercoid. Reverse transcription-polymerase chain reaction and Northern blot analysis demonstrated that the gene was expressed in the coracidium and plerocercoid but not in immature egg and adult. These results suggest that the 27 kDa cysteine protease is only expressed in the stages involving active migration of the parasite in the host tissue.

Citations

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  • Surgical treatment of a patient with live intracranial sparganosis for 17 years
    Jialing Hu, Kaili Liao, Xiaojin Feng, Danling Jiang, Hailin Liu, Qingcui Zheng, Hai Qiu, Fuzhou Hua, Guohai Xu, Chunhua Xu
    BMC Infectious Diseases.2022;[Epub]     CrossRef
  • Follow-up study of high-dose praziquantel therapy for cerebral sparganosis
    Peng Zhang, Yang Zou, Feng-Xia Yu, Zheng Wang, Han Lv, Xue-Huan Liu, He-Yu Ding, Ting-Ting Zhang, Peng-Fei Zhao, Hong-Xia Yin, Zheng-Han Yang, Zhen-Chang Wang, Siddhartha Mahanty
    PLOS Neglected Tropical Diseases.2019; 13(1): e0007018.     CrossRef
  • A cysteine protease from Spirometra erinaceieuropaei plerocercoid is a critical factor for host tissue invasion and migration
    Daigo Tsubokawa, Takeshi Hatta, Hiroki Maeda, Fusako Mikami, Yukinobu Goso, Takeshi Nakamura, M. Abdul Alim, Naotoshi Tsuji
    Acta Tropica.2017; 167: 99.     CrossRef
  • Characterization of Spirometra erinaceieuropaei Plerocercoid Cysteine Protease and Potential Application for Serodiagnosis of Sparganosis
    Li Na Liu, Zhong Quan Wang, Xi Zhang, Peng Jiang, Xin Qi, Ruo Dan Liu, Zi Fang Zhang, Jing Cui, Xiao-Nong Zhou
    PLOS Neglected Tropical Diseases.2015; 9(6): e0003807.     CrossRef
  • Diagnosis and stereotactic aspiration treatment of cerebral sparganosis: summary of 11 cases
    Lei Deng, Pengju Xiong, Suokai Qian
    Journal of Neurosurgery.2011; 114(5): 1421.     CrossRef
  • Identification of host immune regulation candidate genes of Toxascaris leonina by expression sequenced tags (ESTs) analysis
    Min Kyoung Cho, Keun Hee Lee, Sun Joo Lee, Se Won Kang, Mee Sun Ock, Yeon Chul Hong, Yong Seok Lee, Hak Sun Yu
    Veterinary Parasitology.2009; 164(2-4): 242.     CrossRef
  • Comparison of carbohydrate moieties of sparganum proteins of the snake, mouse and those of adult worm
    Hyun Jong Yang
    The Korean Journal of Parasitology.2003; 41(2): 135.     CrossRef
  • 8,857 View
  • 81 Download
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Case Report

A case of chronic cerebral paragonimiasis westermani
Shin-Yong Kang, Tae-Kwon Kim, Tae Yun Kim, Young-Il Ha, Sun-Wook Choi, Sung-Jong Hong
Korean J Parasitol 2000;38(3):167-171.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.167

We report a chronic cerebral paragonimiasis from a 41-year-old Korean man who complains a headache and weakness of left motor neuron components. Magnetic resonance images of the brain revealed conglomerates of multiple ring-like enhancements in temporo-occipital and frontal lobes of the right hemisphere. An intradermal test for paragonimiasis westermani was positive. The patient was born near an endemic area of paragonimiasis and used to eat boiled or grilled freshwater crayfish in his childhood. Nodules in the brain were resected through craniotomies. The eggs of P. westermani were identified pathologically and parasitologically in the calcified necrotic lesions. Examinations on sputum and fecal specimens for the eggs of P. westermani were shown to be negative and a chest radiograph was normal. It is presumed that the brain lesions were formed by P. westermani approximately 30 years ago.

Citations

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  • Imaging of infectious and inflammatory cystic lesions of the brain, a narrative review
    Anna Cervantes-Arslanian, Hector H Garcia, Otto Rapalino
    Expert Review of Neurotherapeutics.2023; 23(3): 237.     CrossRef
  • Cerebral Paragonimiasis Presenting with Dementia
    Seok Woo Moon, Taeho Kim
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Original Article
Persisting antibody reaction in paragonimiasis after praziquantel treatment is elicited mainly by egg antigens
Seung-Yull Cho, Yoon Kong, Doo-Hee Yun, Shin-Yong Kang, Lee-Soo Kim, Young-Bae Chung, Hyun-Jong Yang
Korean J Parasitol 2000;38(2):75-84.
Published online June 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.2.75

Antibody responses in serum and cerebrospinal fluid (CSF) samples from patients with active and chronic paragonimiasis and in sera from patients on whom follow-up studies were done after praziquantel treatment were analyzed using antigens of Paragonimus westermani prepared from eggs, metacercariae, juveniles of 4- and 7-week old, adult worms and recombinant protein of 28 kDa cruzipain-like cysteine protease (rPw28CCP). The patient sera/CSFs of active and chronic paragonimiasis revealed strong antibody reactions against the crude extracts of 4- and 7-week old juveniles as well as against those from egg and adult. rPw28CCP also showed specific reaction to the sera with active paragonimiasis. After the treatment, levels of specific antibodies in the sera gradually decreased to negative range in most patients. In some cases with persisting high antibody levels, however, the reactions at 27 kDa egg protein were sustained throughout the observation period of 34 months. The reactions at 35 and 32 kDa in adult extract and rPw28CCP disappeared rapidly after the treatment. Persistent antibody reactions even after successful treatment are provoked by continuous antigenic challenge from eggs which were not resolved by treatment.

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