Parasites, Hosts and Diseases

"Shunyu Li"

Original Articles

C3H/He Mice as an Incompatible Cholangiocarcinoma Model by Clonorchis sinensis, Dicyclanil and N-Nitrosodimethylamine: Md. Hafiz Uddin, Shunyu Li, Yan Jin, Min-Ho Choi, Ja June Jang, Sung-Tae Hong; Korean J Parasitol 2016;54(3):281-289.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.281

Clonorchis sinensis is a Group-I bio-carcinogen, associated with cholangiocarcinoma (CCA). The hamster is the only experimental model of C. sinensis-mediated CCA, but we oblige another animal model. The present study intended to develop a C. sinensis (Cs) mediated CCA model using C3H/He mice, co-stimulated with N-nitrosodimethyl-amine (NDMA) and dicyclanil (DC). The mice were divided into 8 groups with different combinations of Cs, NDMA, and DC. Six months later the mice were sacrificed and subjected to gross and histopathological examination. The body weights were significantly reduced among the groups treated with 2 or more agents (eg. Cs+NDMA, Cs+DC, NDMA+DC, and Cs+NDMA+DC). In contrast, liver weight percentages to body weight were increased in above groups by 4.1% to 4.7%. A Change of the spleen weight was observed only in Cs+NDMA group. Though C. sinensis infection is evident from hyperplastic changes, only 1 worm was recovered. T wo mice, 1 from Cs and the other from Cs+DC group, showed mass forming lesions; 1 (281.2 mm3) from the Cs group was a hepatocellular adenoma and the other (280.6 mm3) from the Cs+DC group was a cystic mass (peliosis). Higher prevalence of gray-white nodules was observed in Cs group (42.9%) followed by Cs+NDMA+DC group (21.4%). The mice of the Cs+NDMA+DC group showed hyper-proliferation of the bile duct with fibrotic changes. No characteristic change for CCA was recognized in any of the groups. In conclusion, C3H/He mice produce no CCA but extensive fibrosis when they are challenged by Cs, NDMA, and DC together.

Citations

Citations to this article as recorded by

Clonorchis sinensis and Cholangiocarcinoma
Eun-Min Kim, Sung-Tae Hong
Journal of Korean Medical Science.2025;[Epub] CrossRef
Study of gut microbiota in cholangiocarcinoma patients
O. S. Fedorova, A. Е. Kovshirina, T. S. Sokolova, V. V. Kulenich, L. M. Ogorodova
Bulletin of Siberian Medicine.2025; 24(1): 105. CrossRef
Opisthorchis Felineus Infection is a Risk Factor for Cholangiocarcinoma in Western Siberia: A Hospital-based Case-control Study
Olga S Fedorova, Anna E Kovshirina, Yulia V Kovshirina, Jan Hattendorf, Sergey V Onishchenko, Ludmila L Katanakhova, Stanislav S Taslicki, Andrey V Chizhikov, Ilya A Tataurov, Sergey V Vtorushin, Banchob Sripa, Ludmila M Ogorodova, Peter Odermatt
Clinical Infectious Diseases.2023; 76(3): e1392. CrossRef
Similarities and differences among the Opisthorchiidae liver flukes: insights from Opisthorchis felineus
Maria Y. Pakharukova, Viatcheslav A. Mordvinov
Parasitology.2022; 149(10): 1306. CrossRef
The Overactivation of NADPH Oxidase during Clonorchis sinensis Infection and the Exposure to N-Nitroso Compounds Promote Periductal Fibrosis
Ji Hoon Jeong, Junyeong Yi, Myung Ki Hwang, Sung-Jong Hong, Woon-Mok Sohn, Tong-Soo Kim, Jhang Ho Pak
Antioxidants.2021; 10(6): 869. CrossRef
Association between cholangiocarcinoma and liver flukes: review of epidemiological studies
A. E. Kovshirina, O. S. Fedorova, Y. V. Kovshirina, S. V. Onishchenko, S. S. Taslicki, L. L. Katanakhova, A. V. Chizhikov, I. A. Tataurov, L. M. Ororodova
Bulletin of Siberian Medicine.2020; 19(1): 150. CrossRef
TLR2 signal influences the iNOS/NO responses and worm development in C57BL/6J mice infected with Clonorchis sinensis
Qing-Li Yang, Ji-Qing Shen, Zhi-Hua Jiang, Yun-Liang Shi, Xiao-Ling Wan, Yi-Chao Yang
Parasites & Vectors.2017;[Epub] CrossRef

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In Vitro Maintenance of Clonorchis sinensis Adult Worms: Md. Hafiz Uddin, Shunyu Li, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2012;50(4):309-315.
Published online November 26, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.4.309

Abstract
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Clonorchis sinensis is a biological carcinogen inducing human cholangiocarcinoma, and clonorchiasis is one of the important endemic infectious diseases in East Asia. The present study investigated survival longevity of C. sinensis adult worms in various in vitro conditions to find the best way of keeping the worms longer. The worms were maintained in 0.85% NaCl, 1×PBS, 1×Locke's solution, RPMI-1640, DMEM, and IMDM media, and in 1×Locke's solution with different supplements. All of the worms died within 3 and 7 days in 0.85% NaCl and 1×PBS, respectively, but survived up to 57 days in 1×Locke's solution. The worms lived for 106 days in DMEM, and 114 days in both RPMI-1640 and IMDM media. The survival rate in RPMI-1640 medium was the highest (50%) compared to that in DMEM (20±10%) and in IMDM (33.3±25.2%) after 3 months. The 1×Locke's solution with 0.005% bovine bile supplement showed increased duration of maximum survival from 42 days to 70 days. Higher concentration of bile supplements than 0.005% or addition of glucose were disadvantageous for the worm survival. The worms died rapidly in solutions containing L-aspartic acid, L-glutamic acid, and adenine compared to L-arginine, L-serine, and L-tryptophan. In conclusion, the 1×Locke's solution best supports the worms alive among inorganic solutions for 57 days, and the RPMI-1640 medium maintains living C. sinensis adults better and longer up to 114 days in vitro than other media.

Citations

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Clonorchis sinensis and Cholangiocarcinoma
Eun-Min Kim, Sung-Tae Hong
Journal of Korean Medical Science.2025;[Epub] CrossRef
The Truman Show for Human Helminthic Parasites: A Review of Recent Advances in In Vitro Cultivation Platforms
Smita Sutrave, Martin Heinrich Richter
Microorganisms.2023; 11(7): 1708. CrossRef
In vitro culture and morphology of Neoechinorhynchus buttnerae (Eoacanthocephala: Neoechinorhynchidae) collected from the intestine of tambaqui (Colossoma macropomum) farmed in the Brazilian Amazon
M. I. B. Oliveira, C. Majolo, F. A. Sebastião, M. Tavares-Dias, F. R. Brandão, E. C. Chagas
Brazilian Journal of Biology.2023;[Epub] CrossRef
Structure-based virtual screening and molecular dynamics of potential inhibitors targeting sodium-bile acid co-transporter of carcinogenic liver fluke Clonorchis sinensis
Won Gi Yoo, Fuhong Dai, Jhang Ho Pak, Sung-Jong Hong, Jin-Ho Song, Sutas Suttiprapa
PLOS Neglected Tropical Diseases.2022; 16(11): e0010909. CrossRef
Amino acids serve as an important energy source for adult flukes of Clonorchis sinensis
Shan Li, Xueqing Chen, Juanjuan Zhou, Zhizhi Xie, Mei Shang, Lei He, Pei Liang, Tingjin Chen, Qiang Mao, Chi Liang, Xuerong Li, Yan Huang, Xinbing Yu, John Pius Dalton
PLOS Neglected Tropical Diseases.2020; 14(4): e0008287. CrossRef
Sodium-bile acid co-transporter is crucial for survival of a carcinogenic liver fluke Clonorchis sinensis in the bile
Fuhong Dai, Won Gi Yoo, Yanyan Lu, Jin-Ho Song, Ji-Yun Lee, Youngro Byun, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong, John Pius Dalton
PLOS Neglected Tropical Diseases.2020; 14(12): e0008952. CrossRef
Characterization of a novel organic solute transporter homologue from Clonorchis sinensis
Yanyan Lu, Won Gi Yoo, Fuhong Dai, Ji-Yun Lee, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong, John Pius Dalton
PLOS Neglected Tropical Diseases.2018; 12(4): e0006459. CrossRef
Sequence analysis and characterization of pyruvate kinase from Clonorchis sinensis, a 53.1-kDa homopentamer, implicated immune protective efficacy against clonorchiasis
Tingjin Chen, Hongye Jiang, Hengchang Sun, Zhizhi Xie, Pengli Ren, Lu Zhao, Huimin Dong, Mengchen Shi, Zhiyue Lv, Zhongdao Wu, Xuerong Li, Xinbing Yu, Yan Huang, Jin Xu
Parasites & Vectors.2017;[Epub] CrossRef
C3H/He Mice as an Incompatible Cholangiocarcinoma Model by Clonorchis sinensis, Dicyclanil and N-Nitrosodimethylamine
Md. Hafiz Uddin, Shunyu Li, Yan Jin, Min-Ho Choi, Ja June Jang, Sung-Tae Hong
The Korean Journal of Parasitology.2016; 54(3): 281. CrossRef
Clonorchis sinensis acetoacetyl-CoA thiolase: identification and characterization of its potential role in surviving in the bile duct
Jinsi Lin, Hongling Qu, Guishan Chen, Lei He, Yanquan Xu, Zhizhi Xie, Mengyu Ren, Jiufeng Sun, Shan Li, Wenjun Chen, Xueqing Chen, Xiaoyun Wang, Xuerong Li, Chi Liang, Yan Huang, Xinbing Yu
Parasites & Vectors.2015;[Epub] CrossRef
Involvement of PSMD10, CDK4, and Tumor Suppressors in Development of Intrahepatic Cholangiocarcinoma of Syrian Golden Hamsters Induced by Clonorchis sinensis and N-Nitrosodimethylamine
Md. Hafiz Uddin, Min-Ho Choi, Woo Ho Kim, Ja-June Jang, Sung-Tae Hong, Xiao-Nong Zhou
PLOS Neglected Tropical Diseases.2015; 9(8): e0004008. CrossRef
Advanced Enzymology, Expression Profile and Immune Response of Clonorchis sinensis Hexokinase Show Its Application Potential for Prevention and Control of Clonorchiasis
Tingjin Chen, Jinyun Yu, Zeli Tang, Zhizhi Xie, Zhipeng Lin, Hengchang Sun, Shuo Wan, Xuerong Li, Yan Huang, Xinbing Yu, Jin Xu, Aaron R. Jex
PLOS Neglected Tropical Diseases.2015; 9(3): e0003641. CrossRef
Production and Deformation of Clonorchis sinensis Eggs during In Vitro Maintenance
Md. Hafiz Uddin, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong, Jason Mulvenna
PLoS ONE.2012; 7(12): e52676. CrossRef

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Influencing Factors for Cure of Clonorchiasis by Praziquantel Therapy: Infection Burden and CYP3A5 Gene Polymorphism: Chung Hyeon Kim, Jeong-Keun Lee, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2011;49(1):45-49.
Published online March 18, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.1.45

Abstract
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Chemotherapy of clonorchiasis with praziquantel (PZQ) is effective but about 15% of treated cases have been reported uncured. The present study investigated correlation of single nucleotide polymorphisms (SNPs) of the cytochrome P450 gene, CYP3A5 and cure of clonorchiasis. A total of 346 egg passing residents were subjected and treated by 3 doses of 25 mg/kg PZQ. Reexamination recognized 33 (9.5%) uncured and 313 cured. Numbers of eggs per gram of feces (EPGs) before treatment were significantly lower in the cured group than in the uncured group (2,011.2±3,600.0 vs 4,998.5±7,012.0, P<0.001). DNAs of the subjects were screened for SNPs at 7 locations of CYP3A5 using PCR. In the uncured group, the SNP frequencies at g.-20555G>A and g.27526C>T of CYP3A5 were 15.2% and 9.1% while those were 3.8% and 1.0%, respectively, in the cured group. The cure rate was significantly lower in the cases with SNP at g.27526C>T and EPGs≥1,000. In conclusion, EPGs and SNPs of CYP3A5 are factors which influence cure of clonorchiasis by PZQ therapy. It is strongly suggested to recommend 2-day medication for individuals with high EPGs≥1,000.

Citations

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Clonorchiasis and opisthorchiasis: epidemiology, transmission, clinical features, morbidity, diagnosis, treatment, and control
Men-Bao Qian, Jennifer Keiser, Jürg Utzinger, Xiao-Nong Zhou, Graeme N. Forrest
Clinical Microbiology Reviews.2024;[Epub] CrossRef
Efficacy and Safety of Tribendimidine Against Clonorchis sinensis
Men-Bao Qian, Peiling Yap, Yi-Chao Yang, Hai Liang, Zhi-Hua Jiang, Wei Li, Yu-Guang Tan, Hui Zhou, Jürg Utzinger, Xiao-Nong Zhou, Jennifer Keiser
Clinical Infectious Diseases.2013; 56(7): e76. CrossRef
Efficacy of Praziquantel against Schistosoma mekongi and Opisthorchis viverrini: A Randomized, Single-Blinded Dose-Comparison Trial
Leonore Lovis, Tippi K. Mak, Khampheng Phongluxa, Phonepasong Ayé Soukhathammavong, Youthanavanh Vonghachack, Jennifer Keiser, Penelope Vounatsou, Marcel Tanner, Christoph Hatz, Jürg Utzinger, Peter Odermatt, Kongsap Akkhavong, Banchob Sripa
PLoS Neglected Tropical Diseases.2012; 6(7): e1726. CrossRef

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Changing Patterns of Serum and Bile Antibodies in Re-infected Rats with Clonorchis sinensis: Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2008;46(1):17-22.
Published online March 20, 2008; DOI: https://doi.org/10.3347/kjp.2008.46.1.17

Abstract
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Rats develop strong resistance to re-infection and super-infection by Clonorchis sinensis. The present study investigated the antibodies present in the sera and bile juice of rats that were primary infected and re-infected with C. sinensis. The serum level of specific IgG antibodies, which were elevated 2 wk of the primary infection, peaked at 4 wk and subsequently remained unchanged even during re-infection. The total IgE level in serum increased slowly from 388 ng / ml to 3,426 ng / ml beginning 2 wk after the primary infection, and remained high up to 8 wk but dropped to a normal level (259 ng / ml) after treatment. In resistant re-infected rats, the serum IgE level increased rapidly and peaked within 1 wk, whereas no increase was observed in immunosuppressed rats. The serum level of specific IgA antibodies was elevated beginning 1 wk after infection, and decreased 4 wk after treatment. The total bile IgA level unchanged during the primary infection but increased in treated and re-infected rats. The elevated levels of serum IgE and bile IgA indicate that these immunoglobulins may be correlated with the development of resistance to re-infection by C. sinensis in rats.

Citations

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Bacillus subtilis spore with surface display of paramyosin from Clonorchis sinensis potentializes a promising oral vaccine candidate
Hengchang Sun, Zhipeng Lin, Lu Zhao, Tingjin Chen, Mei Shang, Hongye Jiang, Zeli Tang, Xinyi Zhou, Mengchen Shi, Lina Zhou, Pengli Ren, Honglin Qu, Jinsi Lin, Xuerong Li, Jin Xu, Yan Huang, Xinbing Yu
Parasites & Vectors.2018;[Epub] CrossRef
Local immune response to primary infection and re-infection by Clonorchis sinensis in FVB mice
Eun-Min Kim, Hak Sun Yu, Yan Jin, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
Parasitology International.2017; 66(4): 436. CrossRef
Virus-like particles vaccine containing Clonorchis sinensis tegumental protein induces partial protection against Clonorchis sinensis infection
Dong-Hun Lee, Ah-Ra Kim, Su-Hwa Lee, Fu-Shi Quan
Parasites & Vectors.2017;[Epub] CrossRef
Clonorchiasis
Men-Bao Qian, Jürg Utzinger, Jennifer Keiser, Xiao-Nong Zhou
The Lancet.2016; 387(10020): 800. CrossRef
Enhanced protection against Clonorchis sinensis induced by co‐infection with Trichinella spiralis in rats
K.‐B. Chu, S.‐S. Kim, S.‐H. Lee, H.‐S. Lee, K.‐H. Joo, J.‐H. Lee, Y.‐S. Lee, S. Zheng, F.‐S. Quan
Parasite Immunology.2014; 36(10): 522. CrossRef
Systemic and local mucosal immune responses induced by orally delivered Bacillus subtilis spore expressing leucine aminopeptidase 2 of Clonorchis sinensis
Hongling Qu, Yanquan Xu, Hengchang Sun, Jinsi Lin, Jinyun Yu, Zeli Tang, Jiqing Shen, Chi Liang, Shan Li, Wenjun Chen, Xuerong Li, Zhongdao Wu, Yan Huang, Xinbing Yu
Parasitology Research.2014; 113(8): 3095. CrossRef
Clonorchis sinensis and clonorchiasis, an update
Sung-Tae Hong, Yueyi Fang
Parasitology International.2012; 61(1): 17. CrossRef
Strain variation in the susceptibility and immune response to Clonorchis sinensis infection in mice
Md. Hafiz Uddin, Shunyu Li, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong
Parasitology International.2012; 61(1): 118. CrossRef

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Brief Communication

Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis: Pyo Yun Cho, Tae Im Kim, Shunyu Li, Sung-Jong Hong, Min-Ho Choi, Sung-Tae Hong, Yong Je Chung; Korean J Parasitol 2007;45(3):229-232.
Published online September 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.3.229

Abstract
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The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.

Citations

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Clonorchis sinensis and clonorchiasis, an update
Sung-Tae Hong, Yueyi Fang
Parasitology International.2012; 61(1): 17. CrossRef

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67 Download
Crossref

Original Article

Tissue parasitic helminthiases are prevalent at Cheongjin, North Korea: Chenghua Shen, Shunyu Li, Shanzi Zheng, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong; Korean J Parasitol 2007;45(2):139-144.
Published online June 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.2.139

Abstract
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We investigated a small-scale serological survey to screen tissue-parasitic helminthiases of North Koreans as one of research programs for re-unification of Korea. Soil-transmitted helminthiases were found highly prevalent among North Korean residents at the border with China. ELISA using 4 tissue-parasitic helminth antigens was applied to 137 residents living in Cheongjin-shi, Hamgyeongbuk-do, North Korea and 133 female refugees in South Korea in 2004-2005. Among a total of 270 samples, 31 (11.5%), 25 (9.3%), and 11 (4.1%) were positive for specific IgG antibodies to antigens of Clonorchis sinensis, Taenia solium metacestode, and sparganum, respectively. The overall positive rate was 21.5%; 38.2% in males and 15.8% in females. The present finding suggests that tissue parasites, such as C. sinensis, T. solium metacestode and sparganum are highly prevalent in some limited areas of North Korea. These foodborne tissue-parasitic helminthiases should be considered for future control measures of parasitic diseases in North Korea.

Citations

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Relationship between Clonorchis sinensis Infection and Cholangiocarcinoma in Korea
Hwa Sun Kim, Ho-Woo Nam, Hye-Jin Ahn, Dongjae Kim, Yeong Hoon Kim
The Korean Journal of Parasitology.2022; 60(4): 261. CrossRef
Taeniasis and cysticercosis in Asia: A review with emphasis on molecular approaches and local lifestyles
Akira Ito, Tiaoying Li, Toni Wandra, Paron Dekumyoy, Tetsuya Yanagida, Munehiro Okamoto, Christine M Budke
Acta Tropica.2019; 198: 105075. CrossRef
Helminthiases in North Korea: a neglected public health challenge
Eunsuk Chang
Pathogens and Global Health.2019; 113(6): 256. CrossRef
Infections of Soil-Transmitted Helminth in Refugees from North Korea
Young-Il Lee, Min Seo, Suk-Bae Kim
The Korean Journal of Parasitology.2018; 56(3): 291. CrossRef
Porcine cysticercosis (Taenia solium and Taenia asiatica): mapping occurrence and areas potentially at risk in East and Southeast Asia
Uffe Christian Braae, Nguyen Manh Hung, Fadjar Satrija, Virak Khieu, Xiao-Nong Zhou, Arve Lee Willingham
Parasites & Vectors.2018;[Epub] CrossRef
Human Taeniasis in the Republic of Korea: Hidden or Gone?
Jong-Yil Chai
The Korean Journal of Parasitology.2013; 51(1): 9. CrossRef
The global epidemiology of clonorchiasis and its relation with cholangiocarcinoma
Men-Bao Qian, Ying-Dan Chen, Song Liang, Guo-Jing Yang, Xiao-Nong Zhou
Infectious Diseases of Poverty.2012;[Epub] CrossRef

8,862 View
67 Download
Crossref

Brief Communication

Status of intestinal helminthic infections of borderline residents in North Korea: Shunyu Li, Chenghua Shen, Min-Ho Choi, Young Mee Bae, Hiwon Yoon, Sung-Tae Hong; Korean J Parasitol 2006;44(3):265-268.
Published online September 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.3.265

Abstract
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The present authors investigated intestinal parasitic infections among North Korean residents and refugees in China in 2003. The Kato-Katz method was applied to 236 residents and soldiers in a town on the North Korea-China border and to 46 people at a refugee camp in China. Only eggs of Ascaris and Trichuris were detected, with egg positive rates of 41.1% and 37.6%, respectively. The total egg positive rate was 55.0% and most of those who were egg positive were only lightly infected. Women of 61.2% and men of 53.1% were egg positive. The refugees from rural areas showed higher egg positive rates than those from urban areas. The present investigation confirmed high prevalence of soil-transmitted intestinal helminths in rural borderline areas of North Korea.

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Helminthiases in North Korea: a neglected public health challenge
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John J Park, Ah-Young Lim, Hyung-Soon Ahn, Andrew I Kim, Soyoung Choi, David HW Oh, Owen Lee-Park, Sharon Y Kim, Sun Jae Jung, Jesse B Bump, Rifat Atun, Hee Young Shin, Kee B Park
BMJ Global Health.2019; 4(2): e001133. CrossRef
An Analysis of Infectious Disease Research Trends in Medical Journals From North Korea
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North Korean Medical Journals in the Galapagos
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Journal of Korean Medical Science.2018;[Epub] CrossRef
Infections of Soil-Transmitted Helminth in Refugees from North Korea
Young-Il Lee, Min Seo, Suk-Bae Kim
The Korean Journal of Parasitology.2018; 56(3): 291. CrossRef
Characteristics of Body Composition and Muscle Strength of North Korean Refugees during South Korean Stay
Sun Wook Cho, So Hee Lee, Eun Sil Koh, Si Eun Kim, Seok Joong Kim
Endocrinology and Metabolism.2015; 30(4): 551. CrossRef
Maintaining Health Sector Collaborations between United States Non-Governmental Organizations and North Korea through Innovation and Planning
Eugene S. Yim, Ricky Y. Choi, Michael J. VanRooyen
Prehospital and Disaster Medicine.2009; 24(3): 153. CrossRef
Tissue parasitic helminthiases are prevalent at Cheongjin, North Korea
Chenghua Shen, Shunyu Li, Shanzi Zheng, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
The Korean Journal of Parasitology.2007; 45(2): 139. CrossRef
Private Wells and Disaster Preparedness
N. Fuke
Prehospital and Disaster Medicine.2005; 20(S2): S142. CrossRef

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Original Articles

Organ-specific antigens of Clonorchis sinensis: Shunyu Li, Byung-Suk Chung, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2004;42(4):169-174.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.169

Abstract
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This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7- and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26- and 28-kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43- and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.

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Identification of Myoferlin, a Potential Serodiagnostic Antigen of Clonorchiasis, via Immunoproteomic Analysis of Sera From Different Infection Periods and Excretory-Secretory Products of Clonorchis sinensis
Xiao-Xiao Ma, Yang-Yuan Qiu, Zhi-Guang Chang, Jun-Feng Gao, Rui-Ruo Jiang, Chun-Lin Li, Chun-Ren Wang, Qiao-Cheng Chang
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Recombinant adenylate kinase 3 from liver fluke Clonorchis sinensis for histochemical analysis and serodiagnosis of clonorchiasis
Soon Bin Kwon, Paul Kim, Hae Sun Woo, Tae Yun Kim, Ju Yeong Kim, Hye Min Lee, Yun Soo Jang, Eun-Min Kim, Tai-Soon Yong, Baik Lin Seong
Parasitology.2018; 145(12): 1531. CrossRef
Characterization of immunogenic Clonorchis sinensis protein fractions by gel filtration chromatography
Duan Pham Ngoc, Yuji Arimatsu, Sasithorn Kaewkes, Banchob Sripa
Asian Pacific Journal of Tropical Disease.2015; 5(4): 284. CrossRef
Free radicals enzymatically triggered by Clonorchis sinensis excretory–secretory products cause NF-κB-mediated inflammation in human cholangiocarcinoma cells
Joo-Hyun Nam, Ju Hyun Moon, In Ki Kim, Myoung-Ro Lee, Sung-Jong Hong, Joong Ho Ahn, Jong Woo Chung, Jhang Ho Pak
International Journal for Parasitology.2012; 42(1): 103. CrossRef
In Vitro Maintenance of Clonorchis sinensis Adult Worms
Md. Hafiz Uddin, Shunyu Li, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2012; 50(4): 309. CrossRef
Production and Deformation of Clonorchis sinensis Eggs during In Vitro Maintenance
Md. Hafiz Uddin, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong, Jason Mulvenna
PLoS ONE.2012; 7(12): e52676. CrossRef
KOMP'YuTERNYY I EKSPERIMENTAL'NYY ANALIZ ANTIGENNYKh SVOYSTV VITELLINA I KATEPSINA F OPISTHORCHIS FELINEUS
A A Nosova, P A Belavin, D A Afonnikov
Medical academic journal.2012; 12(4): 33. CrossRef
Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis
Shunyu Li, Jung Guk Shin, Pyo Yun Cho, Tae Im Kim, Sung-Tae Hong, Sung-Jong Hong
Parasitology Research.2011; 108(5): 1295. CrossRef
Scientific Opinion on risk assessment of parasites in fishery products

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Differential gene expression profiling in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory-secretory products
Jhang Ho Pak, Dong-Wook Kim, Ju Hyun Moon, Joo-Hyun Nam, Jong-Hyeok Kim, Jung Won Ju, Tong-Soo Kim, Sang-Beom Seo
Parasitology Research.2009; 104(5): 1035. CrossRef
Proteomic analysis of differentially expressed proteins in human cholangiocarcinoma cells treated withClonorchis sinensisexcretory–secretory products
Jhang Ho Pak, Ju Hyun Moon, Seung‐Jun Hwang, Shin‐Hyeong Cho, Sang‐Beom Seo, Tong‐Soo Kim
Journal of Cellular Biochemistry.2009; 108(6): 1376. CrossRef
Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory‐secretory products of Clonorchis sinensis adult worms
Jung‐Won Ju, Hyun‐Na Joo, Myoung‐Ro Lee, Shin‐Hyeong Cho, Hyeng‐Il Cheun, Jung‐Yeon Kim, Young‐Hee Lee, Kwang‐Jun Lee, Woon‐Mok Sohn, Dong‐Min Kim, Il‐Chul Kim, Byoung Chul Park, Tong‐Soo Kim
PROTEOMICS.2009; 9(11): 3066. CrossRef
Bile components and amino acids affect survival of the newly excysted juvenile Clonorchis sinensis in maintaining media
Shunyu Li, Tae Im Kim, Won Gi Yoo, Pyo Yun Cho, Tong-Soo Kim, Sung-Jong Hong
Parasitology Research.2008; 103(5): 1019. CrossRef
Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis
Changling Ma, Xuchu Hu, Fengyu Hu, Yanwen Li, Xiaoxiang Chen, Zhenwen Zhou, Fangli Lu, Jin Xu, Zhongdao Wu, Xinbing Yu
Parasitology Research.2007; 101(2): 419. CrossRef
Clonorchis sinensis: Expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-LPAP homologue
Fengyu Hu, Xinbing Yu, Changling Ma, Hongjuan Zhou, Zhenwen Zhou, Yanwen Li, Fangli Lu, Jin Xu, Zhongdao Wu, Xuchu Hu
Experimental Parasitology.2007; 117(2): 157. CrossRef
Long-term storage of Clonorchis sinensis metacercariae in vitro
Shunyu Li, Hyoun Woo Kang, Min-Ho Choi, Sung-Tae Hong
Parasitology Research.2006; 100(1): 25. CrossRef
Fish-borne parasitic zoonoses: Status and issues
Jong-Yil Chai, K. Darwin Murrell, Alan J. Lymbery
International Journal for Parasitology.2005; 35(11-12): 1233. CrossRef

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Bile duct changes in rats reinfected with Clonorchis sinensis: Dongil Choi, Sung-Tae Hong, Shunyu Li, Byung-Suk Chung, Jae Hoon Lim, Soon-Hyung Lee; Korean J Parasitol 2004;42(1):7-17.
Published online March 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.1.7

Abstract
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This study describes an evaluation of the sonographic, cholangiographic, pathological, and immunological findings, and the protective effect shown by rats reinfected with Clonorchis sinensis. Eight experimental rat groups were, namely, a normal control, a primary infection control, a reinfection I (reinfection 7 week after treatment following 3-week infection), a reinfection II (reinfection 2 week after treatment following 8-week infection), a reinfection III (exploration of the intrahepatic bile ducts 1 week after reinfection 4 week after treatment following 4-week infection), a superinfection, a secondary infection control, and an infection following immunization group. Sonographic and cholangiographic findings showed moderate or marked dilatation of the bile duct confluence in the primary infection control, reinfection II, and secondary infection control groups. Juvenile worms survived in the intrahepatic bile ducts 1 week after reinfection following treatment in the reinfection III group. It was concluded that reinfecting juvenile worms found during the first week following reinfection failed to survive or grow further. Anatomical, pathophysiological, or immunological changes may induce protection from reinfection in rats.

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Gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid-enhanced T1 mapping to evaluate liver damage caused by Clonorchis sinensis
Yanhan Xiang, Ningqin Li, Zhongyang Zhou, Jing Fang, Jinyuan Liao
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Perturbations of Metabolomic Profiling of Spleen From Rats Infected With Clonorchis sinensis Determined by LC-MS/MS Method
Xiaoli Zhang, Xinyi Hu, Rui Chen, Beibei Sun, Yannan Gao, Shanshan Duan, Liyan Liu, Su Han
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Alessandra Gentilini, Mirella Pastore, Fabio Marra, Chiara Raggi
International Journal of Molecular Sciences.2018; 19(10): 2885. CrossRef
In-vivo monitoring of development of cholangiocarcinoma induced with C. sinensis and N-nitrosodimethylamine in Syrian golen hamsters using ultrasonography and magnetic resonance imaging: a preliminary study
Hyunsik Woo, Joon Koo Han, Jung Hoon Kim, Sung-Tae Hong, Md. Hafiz Uddin, Ja-June Jang
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Oxidative stress-mediated mouse liver lesions caused by Clonorchis sinensis infection
Sejung Maeng, Hye Won Lee, Qudsia Bashir, Tae Im Kim, Sung-Jong Hong, Tae Jin Lee, Woon-Mok Sohn, Byoung-Kuk Na, Tong-Soo Kim, Jhang Ho Pak
International Journal for Parasitology.2016; 46(3): 195. CrossRef
Cell Death and Proliferation after Treatment and Reinfection of Clonorchis sinensis in the Sprague-Dawley Rat Bile Duct
Byoung-Hoon Min, Ka-Young Ahn, Haeng-Sook Lee, Soo-Jin Kim, Kyoung-Hwan Joo
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Fas/FasL-dependent apoptosis of hepatocytes induced in rat and patients with Clonorchis sinensis infection
Xiaoli Zhang, Zhanfeng Jin, Rong Da, Yunxia Dong, Wuqi Song, Xiaobei Chen, Qi Huang, Hong Ling, Yang Che, Yihong Li, Fengmin Zhang
Parasitology Research.2008; 103(2): 393. CrossRef
Changing Patterns of Serum and Bile Antibodies in Re-infected Rats with Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2008; 46(1): 17. CrossRef
Factors in the resistance of rats to re-infection and super-infection by Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
Parasitology Research.2008; 102(6): 1111. CrossRef
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Parasitology Research.2007; 101(6): 1557. CrossRef
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Parasitology Research.2005; 97(1): 21. CrossRef

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Development of resistance to reinfection by Clonorchis sinensis in rats: Byung-Suk Chung, Hongman Zhang, Min-Ho Choi, Deogkyu Jeon, Shunyu Li, Mejeong Lee, Sung-Tae Hong; Korean J Parasitol 2004;42(1):19-26.
Published online March 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.1.19

Abstract
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We investigated the induction of resistance to Clonorchis sinensis infection by prior infection in rat and hamster models. Animals were challenged with C. sinensis metacercariae, then treated with praziquantel and reinfected. Worm recovery rate in reinfected animals was used to estimate resistance to reinfection. The determined resistance rates to reinfection in rats and hamsters were 97.7% and 10.3%, respectively. In rats, cure from the primary infection of C. sinensis increased resistant to reinfection, and the greatert the worm burden and the longer the duration of primary infection, the higher was the resistance rate. For primary infection doses of 10, 40 and 100 metacercariae per rat, the resistance rates were 87.4%, 93.8% and 98.4%, respectively. The resistance rates in rats after 2 or 8-week primary infection were 78.7% and 95.3%, respectively. All worms recovered from reinfected rats were immature. When cured rats were administered with methylprednisolone, resistance to reinfection became impaired. These findings indicate that rats develop a high degree of resistance to reinfection by C. sinensis after cure. The growths and maturations of reinfected worms were also impaired.

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Long Non-Coding RNA and mRNA Expression Analysis in Liver of Mice With Clonorchis sinensis Infection
Su Han, Xue-Li Zhang, Xu Jiang, Xiang Li, Jian Ding, Li-Jiao Zuo, Shan-Shan Duan, Rui Chen, Bei-Bei Sun, Xin-Yi Hu, Yan-Nan Gao, Xiao-Li Zhang
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Bacillus subtilis spore with surface display of paramyosin from Clonorchis sinensis potentializes a promising oral vaccine candidate
Hengchang Sun, Zhipeng Lin, Lu Zhao, Tingjin Chen, Mei Shang, Hongye Jiang, Zeli Tang, Xinyi Zhou, Mengchen Shi, Lina Zhou, Pengli Ren, Honglin Qu, Jinsi Lin, Xuerong Li, Jin Xu, Yan Huang, Xinbing Yu
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Local immune response to primary infection and re-infection by Clonorchis sinensis in FVB mice
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Parasitology International.2017; 66(4): 436. CrossRef
Virus-like particles vaccine containing Clonorchis sinensis tegumental protein induces partial protection against Clonorchis sinensis infection
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Clonorchiasis
Men-Bao Qian, Jürg Utzinger, Jennifer Keiser, Xiao-Nong Zhou
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Parasitology International.2012; 61(1): 118. CrossRef
Factors in the resistance of rats to re-infection and super-infection by Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
Parasitology Research.2008; 102(6): 1111. CrossRef
Changing Patterns of Serum and Bile Antibodies in Re-infected Rats with Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2008; 46(1): 17. CrossRef
Susceptibility of experimental animals to reinfection with Clonorchis sinensis
Woon-Mok Sohn, Hongman Zhang, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2006; 44(2): 163. CrossRef
Bile duct changes in rats reinfected with Clonorchis sinensis
Dongil Choi, Sung-Tae Hong, Shunyu Li, Byung-Suk Chung, Jae Hoon Lim, Soon-Hyung Lee
The Korean Journal of Parasitology.2004; 42(1): 7. CrossRef

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Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda): Min-Ho Choi, Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong; Korean J Parasitol 2003;41(3):155-163.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.155

Abstract
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The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.

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Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA: Min-Ho Choi, Il Chan Park, Shunyu Li, Sung-Tae Hong; Korean J Parasitol 2003;41(1):35-39.
Published online March 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.1.35

Abstract
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Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis by ELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis still suffers from cross-reactions, and therefore there is a need to improve the present conventional ELISA. The present study was undertaken to evaluate the diagnostic value of ELISA using excretory-secretory antigen (ESA) instead of crude antigen (CA) of Clonorchis sinensis. The diagnostic sensitivity of ELISA using excretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensis antigen (88.2%). In addition, the specificity of excretory-secretory antigen was found 93.1% while that of crude antigen was 87.8%. In summary, Clonorchis sinensis ESA was found to be a better serodiagnostic antigen than CA for ELISA.

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BMB Reports.2016; 49(11): 590. CrossRef
Towards improved diagnosis of neglected zoonotic trematodes using a One Health approach
Maria Vang Johansen, Tore Lier, Paiboon Sithithaworn
Acta Tropica.2015; 141: 161. CrossRef
Excretory/secretory products of the carcinogenic liver fluke are endocytosed by human cholangiocytes and drive cell proliferation and IL6 production
Sujittra Chaiyadet, Michael Smout, Michael Johnson, Cynthia Whitchurch, Lynne Turnbull, Sasithorn Kaewkes, Javier Sotillo, Alex Loukas, Banchob Sripa
International Journal for Parasitology.2015; 45(12): 773. CrossRef
The serological diagnosis of human clonorchiasis by time-resolved fluoroimmunoassay based on GST2-specific IgG4 detection
Jin Xu, Lexun Wang, Chuanhuan Deng, Xuchu Hu, Ran Li, Tingjin Chen, Dan Ning, Yingsong Wu, Yan Huang, Xuerong Li, Zhongdao Wu, Xinbing Yu, Yonglong Li
Parasitology Research.2014; 113(1): 149. CrossRef
Pediatric Neurocysticercosis: Usefulness of Antibody Response in Cysticidal Treatment Follow-Up
Venkata Subba Rao Atluri, Venkateswara Reddy Gogulamudi, Pratibha Singhi, Niranjan Khandelwal, Lakshmana Swamy Parasa, Nancy Malla
BioMed Research International.2014; 2014: 1. CrossRef
Infection and Cancer: Global Distribution and Burden of Diseases
Jin-Kyoung Oh, Elisabete Weiderpass
Annals of Global Health.2014; 80(5): 384. CrossRef
Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
Dae-Won Kim, Won Gi Yoo, Myoung-Ro Lee, Hye-Won Yang, Yu-Jung Kim, Shin-Hyeong Cho, Won-Ja Lee, Jung-Won Ju
Parasites & Vectors.2014;[Epub] CrossRef
Trichinosis Caused by Ingestion of Raw Soft-Shelled Turtle Meat in Korea
Sang-Rok Lee, Sang-Hoon Yoo, Hyun-Seon Kim, Seung-Ha Lee, Min Seo
The Korean Journal of Parasitology.2013; 51(2): 219. CrossRef
Expression, immunolocalization, and serological reactivity of a novel sphingomyelin phosphodiesterase-like protein, an excretory/secretory antigen from Clonorchis sinensis
Yanwei Huang, Youwei Zheng, Yuzhe Li, Mei Yang, Ting Li, Suxiang Zeng, Xinbing Yu, Huaiqiu Huang, Xuchu Hu
Parasitology Research.2013; 112(6): 2197. CrossRef
Parasitoses hépatobiliaires (à l’exception de l’amibiase, de l’hydatidose, de la bilharziose et de la balantidiose)
A.R. Ndiaye, B. Ndiaye, N.M. Diagne Gueye, F. Klotz
EMC - Hépatologie.2013; 28(3): 1. CrossRef
Development of a real-time PCR assay for the detection of Clonorchis sinensis DNA in gallbladder bile and stone samples from patients with cholecystolithiasis
Tie Qiao, Pei-Ming Zheng, Rui-Hong Ma, Xiao-Bing Luo, Zhen-Liang Luo
Parasitology Research.2012; 111(4): 1497. CrossRef
Molecular characterization and expression of a cysteine protease from Clonorchis sinensis and its application for serodiagnosis of clonorchiasis
Xiaoli Lv, Wenjun Chen, Xiaoyun Wang, Xuerong Li, Jiufeng Sun, Chuanhuan Deng, Jingtao Men, Yanli Tian, Chenhui Zhou, Huali Lei, Chi Liang, Xinbing Yu
Parasitology Research.2012; 110(6): 2211. CrossRef
Theoretical investigation on structural, functional and epitope of a 12 kDa excretory-secretory protein from Toxoplasma gondii
Yap Boon Wooi Tommy, Theam Soon Lim, Rahmah Noordin, Geita Saadatnia, Yee Siew Choong
BMC Structural Biology.2012;[Epub] CrossRef
Coproantigen capture ELISA for detection of Clonorchis sinensis infection in experimentally infected rats
S.M. Mazidur Rahman, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
Parasitology International.2012; 61(1): 203. CrossRef
Clonorchis sinensis and clonorchiasis, an update
Sung-Tae Hong, Yueyi Fang
Parasitology International.2012; 61(1): 17. CrossRef
Trematode Infections
Thomas Fürst, Urs Duthaler, Banchop Sripa, Jürg Utzinger, Jennifer Keiser
Infectious Disease Clinics of North America.2012; 26(2): 399. CrossRef
Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis
S. M. Mazidur Rahman, Young Mee Bae, Sung-Tae Hong, Min-Ho Choi
Parasitology Research.2011; 109(2): 297. CrossRef
Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis
Shunyu Li, Jung Guk Shin, Pyo Yun Cho, Tae Im Kim, Sung-Tae Hong, Sung-Jong Hong
Parasitology Research.2011; 108(5): 1295. CrossRef
Performance of an Enzyme-Linked Immunosorbent Assay for Detection of Clonorchis sinensis Infestation in High- and Low-Risk Groups
Young Jin Kim, Sun Min Lee, Go Eun Choi, Sang Hyun Hwang, Hyung Hoi Kim, Eun Yup Lee, Chulhun L. Chang
Journal of Clinical Microbiology.2010; 48(7): 2365. CrossRef
Evaluation of excretory secretory and 10–30 kDa antigens of Taenia solium Cysticerci by EITB assay for the diagnosis of neurocysticercosis
S. R. V. ATLURI, P. SINGHI, N. KHANDELWAL, N. MALLA
Parasite Immunology.2009; 31(3): 151. CrossRef
Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory‐secretory products of Clonorchis sinensis adult worms
Jung‐Won Ju, Hyun‐Na Joo, Myoung‐Ro Lee, Shin‐Hyeong Cho, Hyeng‐Il Cheun, Jung‐Yeon Kim, Young‐Hee Lee, Kwang‐Jun Lee, Woon‐Mok Sohn, Dong‐Min Kim, Il‐Chul Kim, Byoung Chul Park, Tong‐Soo Kim
PROTEOMICS.2009; 9(11): 3066. CrossRef
Serodiagnostic applicability of recombinant antigens of Clonorchis sinensis expressed by wheat germ cell-free protein synthesis system
Chenghua Shen, Jong-Ae Lee, Sonia Refaat Ahmed Allam, Young Mee Bae, Eun-Taek Han, Satoru Takeo, Takafumi Tsuboi, Sung-Tae Hong, Min-Ho Choi
Diagnostic Microbiology and Infectious Disease.2009; 64(3): 334. CrossRef
Detection ofClonorchis sinensisin stool samples using real-time PCR
E.-M. Kim, J. J. Verweij, A. Jalili, L. van Lieshout, M.-H. Choi, Y. M. Bae, M. K. Lim, S.-T. Hong
Annals of Tropical Medicine & Parasitology.2009; 103(6): 513. CrossRef
Neurocysticercosis immunodiagnosis using Taenia solium cysticerci crude soluble extract, excretory secretory and lower molecular mass antigens in serum and urine samples of Indian children
Subba Rao V. Atluri, P. Singhi, N. Khandelwal, N. Malla
Acta Tropica.2009; 110(1): 22. CrossRef
Antigenic Properties of Cystatin-binding Cysteine Proteinases from Neodiplostomum seoulense
Tae-Yun Kim, Kye-Young Han, Eun-Hee Shin, Jong-Yil Chai
Journal of Parasitology.2008; 94(3): 654. CrossRef
Intraductal papillary neoplasm of the bile duct associated with Clonorchis sinensis infection
Kee-Taek Jang, Seung-Mo Hong, Kyu Taek Lee, Jong Gyun Lee, Seoung Ho Choi, Jin Seok Heo, Dong Wook Choi, Dongil Choi, Jae Hoon Lim
Virchows Archiv.2008; 453(6): 589. CrossRef
Factors in the resistance of rats to re-infection and super-infection by Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
Parasitology Research.2008; 102(6): 1111. CrossRef
Changing Patterns of Serum and Bile Antibodies in Re-infected Rats with Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2008; 46(1): 17. CrossRef
Gallstones and Clonorchis sinensis infection: A hospital‐based case–control study in Korea
Dongil Choi, Jae Hoon Lim, Kyu Taek Lee, Jong Kyun Lee, Seong Ho Choi, Jin Seok Heo, Dong Wook Choi, Kee‐Taek Jang, Nam Yong Lee, Seonwoo Kim, Sung‐Tae Hong
Journal of Gastroenterology and Hepatology.2008;[Epub] CrossRef
Antigenic Properties of Cystatin-binding Cysteine Proteinases from Neodiplostomum seoulense
Tae-Yun Kim, Kye-Young Han, Eun-Hee Shin, Jong-Yil Chai
Journal of Parasitology.2008; 94(3): 654. CrossRef
Clonorchis sinensis: Expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-LPAP homologue
Fengyu Hu, Xinbing Yu, Changling Ma, Hongjuan Zhou, Zhenwen Zhou, Yanwen Li, Fangli Lu, Jin Xu, Zhongdao Wu, Xuchu Hu
Experimental Parasitology.2007; 117(2): 157. CrossRef
Tissue parasitic helminthiases are prevalent at Cheongjin, North Korea
Chenghua Shen, Shunyu Li, Shanzi Zheng, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
The Korean Journal of Parasitology.2007; 45(2): 139. CrossRef
Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis
Changling Ma, Xuchu Hu, Fengyu Hu, Yanwen Li, Xiaoxiang Chen, Zhenwen Zhou, Fangli Lu, Jin Xu, Zhongdao Wu, Xinbing Yu
Parasitology Research.2007; 101(2): 419. CrossRef
Evaluation of a recombinant excretory secretory Haemonchus contortus protein for use in a diagnostic enzyme-linked immunosorbent assay
Xiaojun Li, Aifang Du, Weiming Cai, Yuhui Hou, Linhai Pang, Xiang Gao
Experimental Parasitology.2007; 115(3): 242. CrossRef
Clonorchis sinensis and Opisthorchis viverrini: Development of a mitochondrial-based multiplex PCR for their identification and discrimination
Thanh Hoa Le, Nguyen Van De, David Blair, Paiboon Sithithaworn, Donald P. McManus
Experimental Parasitology.2006; 112(2): 109. CrossRef
Cholangiocarcinoma and Clonorchis sinensis infection: A case–control study in Korea
Dongil Choi, Jae Hoon Lim, Kyu Taek Lee, Jong Kyun Lee, Seong Ho Choi, Jin Seok Heo, Kee-Taek Jang, Nam Yong Lee, Seonwoo Kim, Sung-Tae Hong
Journal of Hepatology.2006; 44(6): 1066. CrossRef
Fish-borne parasitic zoonoses: Status and issues
Jong-Yil Chai, K. Darwin Murrell, Alan J. Lymbery
International Journal for Parasitology.2005; 35(11-12): 1233. CrossRef
The identification of a Clonorchis sinensis gene encoding an antigenic egg protein
Mejeong Lee, Young-Bae Chung, Suk-Keun Lee, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
Parasitology Research.2005; 95(3): 224. CrossRef
Organ-specific antigens of Clonorchis sinensis
Shunyu Li, Byung-Suk Chung, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2004; 42(4): 169. CrossRef
Evaluation of Clonorchis sinensis Recombinant 7-Kilodalton Antigen for Serodiagnosis of Clonorchiasis
Qin-Ping Zhao, Sung-Ung Moon, Hyeong-Woo Lee, Byoung-Kuk Na, Seung-Yull Cho, Yoon Kong, Ming-Sen Jiang, Ai-Hua Li, Tong-Soo Kim
Clinical and Vaccine Immunology.2004; 11(4): 814. CrossRef
Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda)
Min-Ho Choi, Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong
The Korean Journal of Parasitology.2003; 41(3): 155. CrossRef