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"Sun Huh"

Case Report

A case of vocal cord gnathostomiasis diagnosed with sectional morphologies in a histopathological specimen from a Chinese woman living in Korea
Doo Sik Park, Eun Hyun Cho, Kyung Hoon Park, Soo Min Jo, Bumjung Park, Sun Huh
Parasites Hosts Dis 2023;61(3):298-303.
Published online August 21, 2023
DOI: https://doi.org/10.3347/PHD.23065
This study aimed to describe a rare case of gnathostomiasis in the vocal cord. A 54-year-old Chinese woman living in Korea visited with a chief complaint of voice change at the outpatient department of otorhinolaryngology in Hallym Sacred Heart Hospital, Hallym University on August 2, 2021. She had eaten raw conger a few weeks before the voice change developed, but her medical history and physical examinations demonstrated neither gastrointestinal symptoms nor other health problems. A round and red cystic lesion, recognized in the anterior part of the right vocal cord, was removed using forceps and scissors through laryngeal microsurgery. The histopathological specimen of the cyst revealed 3 cross-sections of a nematode larva in the lumen of the cyst wall composed of inflammatory cells and fibrotic tissues. They differ in diameter, from 190 μm to 235 μm. They showed characteristic cuticular layers with tegumental spines, somatic muscle layers, and gastrointestinal tracts such as the esophagus and intestine. Notably, intestinal sections consisted of 27-28 lining cells containing 0-4 nuclei per cell. We tentatively identified the nematode larva recovered from the vocal cord cystic lesion as the third-stage larva of Gnathostoma, probably G. nipponicum or G. hispidum, based on the sectional morphologies.

Citations

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  • Imported parasitic diseases in the Republic of Korea: status and issues
    Jong-Yil Chai
    Journal of the Korean Medical Association.2025; 68(1): 52.     CrossRef
  • Zoonotic Agents in Farmed Fish: A Systematic Review from the Interdisciplinary Perspective of the One Health Concept
    Juliana Rosa Carrijo Mauad, Marcelo Corrêa da Silva, Carolina Marques Costa Araújo, Rosilda Mara Mussury Franco Silva, Silvia Morales de Queiroz Caleman, Márcia Regina Russo
    Veterinary Sciences.2025; 12(5): 437.     CrossRef
  • 5,336 View
  • 96 Download
  • 2 Web of Science
  • Crossref

Brief Communication

Molecular Identification of Anisakis Larvae Extracted by Gastrointestinal Endoscopy from Health Check-up Patients in Korea
Hyemi Song, Bong-Kwang Jung, Jaeeun Cho, Taehee Chang, Sun Huh, Jong-Yil Chai
Korean J Parasitol 2019;57(2):207-211.
Published online April 30, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.2.207
Anisakiasis is a zoonotic disease induced by anisakid nematodes, and endoscopic inspection is used for a diagnosis or remedy for it. Anisakis simplex, Anisakis physeteris, and Pseudoterranova decipiens had been reported to be the major species causing human infections, particularly, in Japan. However, in Korea, recent studies strongly suggested that Anisakis pegreffii is the major species of human infections. To support this suggestion, we collected anisakid larvae (n=20) from 20 human patients who were undergone gastrointestinal endoscopy at a health check-up center in Korea, and molecular identification was performed on the larvae using PCR-RFLP analysis and gene sequencing of rDNA ITS regions and mtDNA cox2. In addition, anisakid larvae (n=53) collected from the sea eel (Astroconger myriaster) were also examined for comparison with those extracted from humans. The results showed that all human samples (100%) were identified as A. pegreffii, whereas 90.7% of the samples from the sea eel were A. pegreffii with the remaining 9.3% being Hysterothylacium aduncum. Our study confirmed that A. pegreffii is the predominant species causing human anisakiasis in Korea, and this seems to be due to the predominance of this larval type in the fish (sea eels) popularly consumed by the Korean people. The possibility of human infection with H. aduncum in Korea is also suggested.

Citations

Citations to this article as recorded by  Crossref logo
  • Risk Factors of Anisakidosis at the Global Level: A Review
    Yosuke Fujisawa, Diane P. Barton, Shokoofeh Shamsi
    Current Clinical Microbiology Reports.2025;[Epub]     CrossRef
  • Alterations in immunized antigens of Anisakis pegreffii by ampicillin-induced gut microbiome changes in mice
    Myungjun Kim, Jun Ho Choi, Myung-hee Yi, Singeun Oh, Tai-Soon Yong, Ju Yeong Kim
    Parasites, Hosts and Diseases.2024; 62(3): 351.     CrossRef
  • Genetic analyses of Anisakis pegreffii (Nematoda: Anisakidae) from the East Asian finless porpoise Neophocaena asiaeorientalis sunameri (Cetacea: Phocoenidae) in Korean waters
    Sunmin Kim, Jong Yoon Jeon, Kyunglee Lee, Hyunjoo Lee, Han Chan Park, Kyung Eun Lee, Hang Lee, Sung Bin Lee, Sang Wha Kim, Se Chang Park, Seongjun Choe, Heejeong Youn
    Parasitology Research.2024;[Epub]     CrossRef
  • Phylogenetic and phylogeographic analyses of Anisakis simplex sensu stricto (Nematoda: Anisakidae) from the common minke whale in Korean waters
    Sunmin Kim, Bom Sok Lee, Seongjun Choe
    Parasites, Hosts and Diseases.2023; 61(3): 240.     CrossRef
  • Sensitivity of candling as routine method for the detection and recovery of ascaridoids in commercial fish fillets
    E. Mercken, I. Van Damme, B. Šoba, S. Vangeenberghe, A. Serradell, T. De Sterck, J. P. L. Lumain, S. Gabriël
    Scientific Reports.2022;[Epub]     CrossRef
  • Intraspecific Genetic Variation of Anisakis typica in Indian Mackerel Caught from the Gulf of Thailand, Samut Songkhram Province
    Tanawat Chaiphongpachara, Poom Adisakwattana, Nantana Suwandittakul, Daiji Endoh
    The Scientific World Journal.2022; 2022: 1.     CrossRef
  • Anisakiasis Annual Incidence and Causative Species, Japan, 2018–2019
    Hiromu Sugiyama, Mitsuko Shiroyama, Ikuyo Yamamoto, Takashi Ishikawa, Yasuyuki Morishima
    Emerging Infectious Diseases.2022; 28(10): 2105.     CrossRef
  • Identification and genetic characterization of Contracaecum sp. (Nematoda: Anisakidae) from China
    Qingxun ZHANG, Meng MENG, Chengmei HUANG, Shengyong FENG, Jie LIU, Yan TANG, Yu FAN, Guohui YUAN, Shuyi HAN, Jing LUO, Baohua ZHAO, Hongxuan HE
    Integrative Zoology.2021; 16(6): 929.     CrossRef
  • Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System
    Jun Ho Choi, Ju Yeong Kim, Myung-hee Yi, Myungjun Kim, Tai-Soon Yong, Kalman Imre
    BioMed Research International.2021;[Epub]     CrossRef
  • Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish
    Vipavinee Cheypanya, Pheravut Wongsawad, Chalobol Wongsawad, Nattawadee Nantarat
    Asian Pacific Journal of Tropical Medicine.2021; 14(5): 214.     CrossRef
  • Presence of Anisakidae in commercial fish species imported into the Belgian food markets: A systematic review and meta-analyses
    E. Mercken, I. Van Damme, A. Serradell, S. Gabriël
    International Journal of Food Microbiology.2020; 318: 108456.     CrossRef
  • Occurrence and molecular identification of Anisakis larval type 1 (Nematoda: Anisakidae) in marketed fish in Egypt
    Eman Mostafa, Marwa Omar, Shimaa. S. Hassan, Mohamed Samir
    Journal of Parasitic Diseases.2020; 44(3): 536.     CrossRef
  • Ascaridoids in commercial fish: Occurrence, intensity and localization in whole fish and fillets destined for the Belgian market
    E. Mercken, I. Van Damme, S. Vangeenberghe, A. Serradell, T. De Sterck, J.P.L. Lumain, S. Gabriël
    International Journal of Food Microbiology.2020; 327: 108657.     CrossRef
  • Advances in Omic Studies Drive Discoveries in the Biology of Anisakid Nematodes
    Stefano D’Amelio, Fabrizio Lombardo, Antonella Pizzarelli, Ilaria Bellini, Serena Cavallero
    Genes.2020; 11(7): 801.     CrossRef
  • Status of common parasitic diseases in Korea in 2019
    Sun Huh
    Journal of the Korean Medical Association.2019; 62(8): 437.     CrossRef
  • Parasitic infections and medical expenses according to Health Insurance Review Assessment claims data in South Korea, 2011–2018
    Ju Yeong Kim, Myung-hee Yi, Tai-Soon Yong, Guilherme L. Werneck
    PLOS ONE.2019; 14(11): e0225508.     CrossRef
  • Anisakid Larvae from Anchovies in the South Coast of Korea
    Taehee Chang, Bong-Kwang Jung, Sooji Hong, Hyejoo Shin, Jeonggyu Lee, Laddawan Patarwut, Jong-Yil Chai
    The Korean Journal of Parasitology.2019; 57(6): 699.     CrossRef
  • 8,856 View
  • 169 Download
  • 17 Web of Science
  • Crossref

Original Articles

Molecular Genetic Findings of Spirometra decipiens and S. ranarum in Korea
Hyeong-Kyu Jeon, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai, Keeseon S. Eom
Korean J Parasitol 2018;56(4):359-364.
Published online August 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.4.359
The taxonomy of Spirometra species has been controversial despite the medical and veterinary importance. Currently, only a few Spirometra species are considered valid species in the genus Spirometra. In the present study, the distribution of Spirometra species obtained from animals in Korea were identified by molecular analysis of the mitochondrial cytochrome c oxidase I (cox1) gene. A total of 28 Spirometra species specimens were analyzed. These were all collected between 1973 and 2008 in the Republic of Korea. Mitochondrial cox1 sequences were examined for a total of 28 specimens comprising 14 S. decipiens and 14 S. ranarum. The difference in partial cox1 sequences (316 bp) between S. erinaceieuropaei (KJ599680) and S. ranarum (this study) was 9.3%, while that between S. decipiens (KJ599679) and S. ranarum (this study) was 2.2%. Genetic analyses identified 2 Spirometra species in animals such as cat, leopard cat, dog, duck and snake in Korea as S. decipiens and S. ranarum. S. decipiens and S. ranarum were present in Gyeongnam Province (P), Jeonnam P, Gangwon P, Chungbuk P, and Seoul. S. decipiens was found in tadpoles, snakes, ducks, cats, leopard cats and dogs, while S. ranarum was found in cats and dogs. The ratio of S. decipiens:S. ranarum calculated from the molecular data was 14:14 (or 1:1). These results indicate that S. decipiens and S. ranarum are sympatrically distributed in Korea.

Citations

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  • A Global Review of the Zoonotic Potential and Disease Risks of Amphibian Parasites in Bullfrog Aquaculture
    Meiqi Weng, Xinhua Liu, Chenxi Zhang, Rui Shu, Andrew Wang, Haotian Zhang, Xingqiang Wang, Huirong Yang, Jinyong Zhang
    Reviews in Aquaculture.2025;[Epub]     CrossRef
  • Description of Spirometra asiana sp. nov. (Cestoda: Diphyllobothriidae) found in wild boars and hound dogs in Japan
    Hiroshi Yamasaki, Hiromu Sugiyama, Yasuyuki Morishima, Hirotaka Kobayashi
    Parasitology International.2024; 98: 102798.     CrossRef
  • Identification of a Clinical Spirometra mansoni Plerocercoid Isolate Using Molecular and Morphological Data
    Si Si Ru, Chi Cheng, Peng Jiang, Xi Zhang
    Acta Parasitologica.2024; 69(2): 1304.     CrossRef
  • Metabarcoding of pathogenic parasites based on copro-DNA analysis of wild animals in South Korea
    Jun Ho Choi, Soo Lim Kim, Dong Kyun Yoo, Myung-hee Yi, Singeun Oh, Myungjun Kim, Sohyeon Yun, Tai-Soon Yong, Seongjun Choe, Jong Koo Lee, Ju Yeong Kim
    Heliyon.2024; 10(9): e30059.     CrossRef
  • Epidemiology, Diagnosis, and Prevention of Sparganosis in Asia
    Wei Liu, Tengfang Gong, Shuyu Chen, Quan Liu, Haoying Zhou, Junlin He, Yong Wu, Fen Li, Yisong Liu
    Animals.2022; 12(12): 1578.     CrossRef
  • Morphological Characteristics and Molecular Phylogenetic Evidence Support the Existence of a New Spirometra Species, Spirometra Asiana, (Cestoda: Diphyllobothriidae) in the Wild Boar
    Hiroshi Yamasaki, Hiromu Sugiyama, Yasuyuki Morishima
    SSRN Electronic Journal .2022;[Epub]     CrossRef
  • Spirometra species from Asia: Genetic diversity and taxonomic challenges
    Hiroshi Yamasaki, Oranuch Sanpool, Rutchanee Rodpai, Lakkhana Sadaow, Porntip Laummaunwai, Mesa Un, Tongjit Thanchomnang, Sakhone Laymanivong, Win Pa Pa Aung, Pewpan M. Intapan, Wanchai Maleewong
    Parasitology International.2021; 80: 102181.     CrossRef
  • First Clinical Cases of Spirometrosis in Two Cats in Korea
    Joohyung Kim, Younsung Ock, Kihwan Yang, Seongjun Choe, Kyung-Mee Park, Wan-Kyu Lee, Kyung-Chul Choi, Soochong Kim, Dongmi Kwak, Seung-Hun Lee
    The Korean Journal of Parasitology.2021; 59(2): 153.     CrossRef
  • A Case of Sparganosis Caused by Spirometra decipiens on the Femur
    Shoko YOGO, Sakurako IMAMURA, Kiko NAGAI, Kayo HARADA, Eiji NAGAYASU, Haruhiko MARUYAMA, Kazunori URABE
    Nishi Nihon Hifuka.2021; 83(5): 453.     CrossRef
  • Low prevalence of spargana infection in farmed frogs in the Yangtze River Delta of China
    Xiaoli Zhang, Rongsheng Mi, Yehua Zhang, Shijie Zhang, Tao Sun, Haiyan Jia, Yan Huang, Haiyan Gong, Xiangan Han, Zhaoguo Chen
    Infection, Genetics and Evolution.2020; 85: 104466.     CrossRef
  • Complete Sequence of the Mitochondrial Genome of Spirometra ranarum: Comparison with S. erinaceieuropaei and S. decipiens
    Hyeong-Kyu Jeon, Hansol Park, Dongmin Lee, Seongjun Choe, Yeseul Kang, Mohammed Mebarek Bia, Sang-Hwa Lee, Keeseon S. Eom
    The Korean Journal of Parasitology.2019; 57(1): 55.     CrossRef
  • Genetic diversity of two mitochondrial DNA genes inSpirometra erinaceieuropaei(Cestoda: Diphyllobothridae) from Poland
    Marta Kołodziej‐Sobocińska, Joanna Stojak, Eliza Kondzior, Iwona Ruczyńska, Jan M. Wójcik
    Journal of Zoological Systematics and Evolutionary Research.2019; 57(4): 764.     CrossRef
  • 8,533 View
  • 126 Download
  • 10 Web of Science
  • Crossref
Draft Genome of Toxocara canis, a Pathogen Responsible for Visceral Larva Migrans
Jinhwa Kong, Jungim Won, Jeehee Yoon, UnJoo Lee, Jong-Il Kim, Sun Huh
Korean J Parasitol 2016;54(6):751-758.
Published online December 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.6.751
This study aimed at constructing a draft genome of the adult female worm Toxocara canis using next-generation sequencing (NGS) and de novo assembly, as well as to find new genes after annotation using functional genomics tools. Using an NGS machine, we produced DNA read data of T. canis. The de novo assembly of the read data was performed using SOAPdenovo. RNA read data were assembled using Trinity. Structural annotation, homology search, functional annotation, classification of protein domains, and KEGG pathway analysis were carried out. Besides them, recently developed tools such as MAKER, PASA, Evidence Modeler, and Blast2GO were used. The scaffold DNA was obtained, the N50 was 108,950 bp, and the overall length was 341,776,187 bp. The N50 of the transcriptome was 940 bp, and its length was 53,046,952 bp. The GC content of the entire genome was 39.3%. The total number of genes was 20,178, and the total number of protein sequences was 22,358. Of the 22,358 protein sequences, 4,992 were newly observed in T. canis. Following proteins previously unknown were found: E3 ubiquitin-protein ligase cbl-b and antigen T-cell receptor, zeta chain for T-cell and B-cell regulation; endoprotease bli-4 for cuticle metabolism; mucin 12Ea and polymorphic mucin variant C6/1/40r2.1 for mucin production; tropomodulin-family protein and ryanodine receptor calcium release channels for muscle movement. We were able to find new hypothetical polypeptides sequences unique to T. canis, and the findings of this study are capable of serving as a basis for extending our biological understanding of T. canis.

Citations

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  • Parasites in Sewage: Legal Requirements and Diagnostic Tools
    Oliwia Obuch-Woszczatyńska, Klaudia Bylińska, Małgorzata Krzyżowska, Karol Korzekwa, Piotr Bąska
    Pathogens.2025; 14(1): 86.     CrossRef
  • Toward anthelmintic drug candidates for toxocariasis: Challenges and recent developments
    Ana C. Mengarda, Tais C. Silva, Aline S. Silva, Daniel B. Roquini, João Paulo S. Fernandes, Josué de Moraes
    European Journal of Medicinal Chemistry.2023; 251: 115268.     CrossRef
  • Antigenic Proteins from the Excretory–Secretory Products of Toxocara canis Larvae and Evaluation of Their Potential for Immunodiagnostics of Larval Toxocarosis
    Kateřina Skulinová, Jan Novák, Libuše Kolářová, Martin Kašný
    Acta Parasitologica.2022; 67(2): 705.     CrossRef
  • GAAP: A Genome Assembly + Annotation Pipeline
    Jinhwa Kong, Sun Huh, Jung-Im Won, Jeehee Yoon, Baeksop Kim, Kiyong Kim
    BioMed Research International.2019; 2019: 1.     CrossRef
  • 15,015 View
  • 175 Download
  • 6 Web of Science
  • Crossref
Human Infections with Spirometra decipiens Plerocercoids Identified by Morphologic and Genetic Analyses in Korea
Hyeong-Kyu Jeon, Hansol Park, Dongmin Lee, Seongjun Choe, Kyu-Heon Kim, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai, Keeseon S. Eom
Korean J Parasitol 2015;53(3):299-305.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.299
Tapeworms of the genus Spirometra are pseudophyllidean cestodes endemic in Korea. At present, it is unclear which Spirometra species are responsible for causing human infections, and little information is available on the epidemiological profiles of Spirometra species infecting humans in Korea. Between 1979 and 2009, a total of 50 spargana from human patients and 2 adult specimens obtained from experimentally infected carnivorous animals were analyzed according to genetic and taxonomic criteria and classified as Spirometra erinaceieuropaei or Spirometra decipiens depending on the morphology. Morphologically, S. erinaceieuropaei and S. decipiens are different in that the spirally coiled uterus in S. erinaceieuropaei has 5-7 complete coils, while in S. decipiens it has only 4.5 coils. In addition, there is a 9.3% (146/1,566) sequence different between S. erinaceieuropaei and S. decipiens in the cox1 gene. Partial cox1 sequences (390 bp) from 35 Korean isolates showed 99.4% (388/390) similarity with the reference sequence of S. erinaceieuropaei from Korea (G1724; GenBank KJ599680) and an additional 15 Korean isolates revealed 99.2% (387/390) similarity with the reference sequences of S. decipiens from Korea (G1657; GenBank KJ599679). Based on morphologic and molecular databases, the estimated population ratio of S. erinaceieuropaei to S. decipiens was 35: 15. Our results indicate that both S. erinaceieuropaei and S. decipiens found in Korea infect humans, with S. erinaceieuropaei being 2 times more prevalent than S. decipiens. This study is the first to report human sparganosis caused by S. decipiens in humans in Korea.

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  • Molecular confirmation of Spirometra mansoni (Cestoda: Diphyllobothriidae) in Javanese keelback water snake (Fowlea melanzosta) in Indonesia
    Ryanka Edila, Seongjun Choe, Mustofa Helmi Effendi, Lucia Tri Suwanti, John Yew Huat Tang
    International Journal for Parasitology: Parasites and Wildlife.2025; 28: 101150.     CrossRef
  • A case of cerebral sparganosis diagnosed by surgical resection and molecular analysis
    Ryo Miyahara, Osamu Akiyama, Naoko Yoshida, Mai Suzuki, Karin Ashizawa, Takuma Kodama, Yuzaburo Shimizu, Akihide Kondo
    Surgical Neurology International.2025; 16: 512.     CrossRef
  • Description of Spirometra asiana sp. nov. (Cestoda: Diphyllobothriidae) found in wild boars and hound dogs in Japan
    Hiroshi Yamasaki, Hiromu Sugiyama, Yasuyuki Morishima, Hirotaka Kobayashi
    Parasitology International.2024; 98: 102798.     CrossRef
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    Acta Parasitologica.2024; 69(2): 1304.     CrossRef
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    Roman Kuchta, Anna J. Phillips, Tomáš Scholz
    International Journal for Parasitology: Parasites and Wildlife.2024; 24: 100947.     CrossRef
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    The Korean Journal of Parasitology.2021; 59(2): 139.     CrossRef
  • First Clinical Cases of Spirometrosis in Two Cats in Korea
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    The Korean Journal of Parasitology.2021; 59(2): 153.     CrossRef
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    Parasitology International.2021; 84: 102383.     CrossRef
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  • A Case of Sparganosis Caused by Spirometra decipiens on the Femur
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Brief Communication

To determine the cause of death of prisoners of war during the Korean War (1950-1953), death certificates or medical records were analyzed. Out of 7,614 deaths, 5,013 (65.8%) were due to infectious diseases. Although dysentery and tuberculosis were the most common infectious diseases, parasitic diseases had caused 14 deaths: paragonimiasis in 5, malaria in 3, amoebiasis in 2, intestinal parasitosis in 2, ascariasis in 1, and schistosomiasis in 1. These results showed that paragonimiasis, malaria, and amoebiasis were the most fatal parasitic diseases during the early 1950s in the Korean Peninsula. Since schistosomiasis is not endemic to Korea, it is likely that the infected private soldier moved from China or Japan to Korea.

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Original Articles

Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae
Yan Jin, Chenghua Shen, Sun Huh, Woon-Mok Sohn, Min-Ho Choi, Sung-Tae Hong
Korean J Parasitol 2013;51(4):433-439.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.433

Toxocariasis is a worldwide zoonosis caused by larvae of ascarid nematodes of dogs or cats, Toxocara canis or T. cati. Diagnosis of human toxocariasis currently relies on serology that uses T. canis excretory-secretory antigen to detect specific IgG antibodies by ELISA. We investigated the serodiagnostic efficacy of ELISA using crude antigen of T. canis larvae (TCLA). Serum specimens of 64 clinically confirmed toxocariasis, 115 healthy controls, and 119 other tissue-invading helminthiases were screened by ELISA using TCLA. The ELISA using TCLA showed 92.2% (59/64 patient samples) sensitivity and 86.6% (103/119) specificity. Its positive diagnostic predictivity was 78.7% and negative predictivity was 97.8%. No serum of healthy controls reacted but that of anisakiasis (45.5%), gnathostomiasis (19.2%), clonorchiasis (15.8%), sparganosis (11.1%), and cysticercosis (6.3%) cross-reacted. Immunoblot analysis on TCLA recognized antigenic proteins of 28- and 30-kDa bands in their dominant protein quantity and strong blotting reactivity. The present results indicate that the ELISA using our TCLA antigen is acceptable by the sensitivity and specificity for serodiagnosis of human toxocariasis. ELISA with TCLA is recommended to make differential diagnosis for patients with any sign of organ infiltration and eosinophilia.

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Dongil Choi, Jae Hoon Lim, Dong-Chull Choi, Kyung Soo Lee, Seung Woon Paik, Sun-Hee Kim, Yoon-Ho Choi, Sun Huh
Korean J Parasitol 2012;50(1):23-27.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.23

The aim of this study is to ascertain the relationship between ingestion of raw cow liver and Toxocara canis infection. A total of 150 apparently healthy adults were divided into 2 groups; 1 group consisted of 86 adults with positive results of Toxocara ELISA, and the other group of 64 adults with negative results. One researcher collected the history of ingestion of raw cow liver within 1 year and recent history of keeping dogs. Among 86 seropositive adults for T. canis, 68 (79.1%) had a recent history of ingestion of raw cow liver. Multivariate statistical analysis showed that a recent ingestion of raw cow liver and keeping dogs were related to an increased risk of toxocariasis (odds ratios, 4.4 and 3.7; and 95% confidence intervals, 1.9-10.2 and 1.2-11.6, respectively). A recent history of ingestion of raw cow liver and keeping dogs was significantly associated with toxocariasis.

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Morphologic and Genetic Identification of Diphyllobothrium nihonkaiense in Korea
Hyeong-Kyu Jeon, Kyu-Heon Kim, Sun Huh, Jong-Yil Chai, Duk-Young Min, Han-Jong Rim, Keeseon S. Eom
Korean J Parasitol 2009;47(4):369-375.
Published online December 1, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.4.369

Diphyllobothrium nihonkaiense was first described by Yamane in 1986 but the taxonomical features have been obscure due to lack of critical morphologic criteria in its larval and adult stages. In Korea, this tapeworm had long been known as Diphyllobothrium latum. In this study, we observed 62 specimens collected from Korean residents and analyzed them by morphological features and nucleotide sequences of mitochondrial cox1 gene as well as the ITS1 region. Adult tapeworms were examined after carmine or trichrome stain. Longitudinal sections of the gravid proglottids showed an obtuse angle of about 150 degree between the cirrus sac and seminal vesicle. This angle is known as a major differential point compared with that of D. latum. Nucleotide sequence differences between D. latum and the specimens from Koreans represented 17.3% in mitochondrial DNA cox1 gene. Sequence divergence of ITS1 among 4 Korean isolates was 0.3% and similarity was 99.7% with D. nihonkaiense and D. klebanovskii. All of the Korean specimens analyzed in this study were identified as being D. nihonkaiense (n = 62). We propose its Korean name as "Dong-hae-gin-chon-chung" which means 'long tapeworm of the East Sea' for this newly analyzed diphyllobothriid tapeworm in Korea.

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Jong-Kyu Lee, Eun-Taek Han, Sun Huh, Woo-Yoon Park, Jae-Ran Yu
Korean J Parasitol 2009;47(3):219-225.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.219

The seroprevalence of cryptosporidiosis was examined using patients' sera collected from hospitals located in 4 different areas of the Republic of Korea. ELISA was used to measure antibody titers against Cryptosporidium parvum antigens from a total of 2,394 serum samples, which were collected randomly from patients in local hospitals; 1) Chungbuk National University Hospital, 2) Konkuk University Hospital, 3) local hospitals in Chuncheon, Gangwon-do (province), 4) Jeonnam National University Hospital, from 2002 through 2003. Of the 2,394 samples assayed, 34%, 26%, and 56% were positive for C. parvum-specific IgG, IgM, and IgA antibodies, respectively. Positive IgG titers were most common in sera from Jeonnam National University Hospital, Gwangju, Jeollanam-do, and positive IgM titers were most common in sera from Chungbuk National University Hospital, Cheongju, Chuncheongbuk-do. The seropositivity was positively correlated with age for both the IgG and IgA antibodies but was negatively correlated with age for the IgM antibodies. Western blotting revealed that 92%, 83%, and 77% of sera positive for IgG, IgM, and IgA ELISA reacted with 27-kDa antigens, respectively. These results suggested that infection with Cryptosporidium in hospital patients occurs more commonly than previously reported in the Republic of Korea.

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Brief Communication

Ultrastructural Localization of Toxocara canis Larval Antigen Reacted with a Seropositive Human Serum
Soo-Ung Lee, Jae-Ran Yu, Sun Huh
Korean J Parasitol 2009;47(1):65-68.
Published online March 12, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.1.65

Excretory-secretory products of Toxocara canis larvae have been considered as a major functional antigen in immune responses against toxocariasis. We studied ultrastructural localization of T. canis second-stage larval antigen using a seropositive human serum under immunogold electron microscopy. High-density gold particles were observed in the secretory cells, excretory duct, intestinal epithelium, and cuticle of the larval worm sections. The distribution of the positive reactions in the larval worms suggests that the nature of the antigen is excretory-secretory antigen including waste metabolites and secretory enzymes.

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Original Articles

A Nationwide Survey on the Prevalence of Intestinal Parasitic Infections in the Republic of Korea, 2004
Tong-Soo Kim, Shin-Hyeong Cho, Sun Huh, Yoon Kong, Woon-Mok Sohn, Seung-Sik Hwang, Jong-Yil Chai, Soon-Hyung Lee, Yun-Kyu Park, Dae-Kyu Oh, Jong-Koo Lee
Korean J Parasitol 2009;47(1):37-47.
Published online March 12, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.1.37

National surveys on the prevalence of intestinal parasitic infections have been carried out every 5-7 years since 1971 in the Republic of Korea in order to establish control measures. The present nationwide survey was conducted from June to December 2004. The 10% population sampling data of Population and Housing Census by the Korean government in 2000 was used as the survey population. One sample was selected randomly from each of the 22,858 registered subjects, and a total of 20,541 people were ultimately included in this survey. Fecal examinations were performed by the cellophane thick smear and saturated brine flotation techniques. Pinworm infection was examined by cello-tape anal swab method. This survey also included a questionnaire study for a socioeconomic analysis. The total helminth egg positive rate was 3.7%, and the estimated total positive number among nationwide people was 1,780,000. The rates in urban and rural areas were 3.1% and 6.8%, respectively. As the total egg positive rate in the 6th survey in 1997 was 2.4%, the present survey showed that there was a considerable degree of increase in the prevalence rate of intestinal parasitic infections over the 7-year period following the 6th survey. The largest increases occurred in the egg positive rates of Clonorchis sinensis and heterophyids including Metagonimus yokogawai.

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Toxocariasis and Ingestion of Raw Cow Liver in Patients with Eosinophilia
Dongil Choi, Jae Hoon Lim, Dong-Chull Choi, Seung Woon Paik, Sun-Hee Kim, Sun Huh
Korean J Parasitol 2008;46(3):139-143.
Published online September 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.3.139

Ingestion of raw animal liver has been suggested as a possible mode of infection of human toxocariasis. We evaluated the relationship between toxocariasis and the ingestion of raw meat in patients with eosinophilia of unknown etiology. The study population consisted of 120 patients presenting with peripheral blood eosinophilia (> 500 cells/?l or > 10% of the white blood cell count). They were divided into 2 groups: 104 seropositive patients based on a Toxocara excretory-secretory IgG ELISA and 16 seronegative patients. While 25.0% of seronegative patients had a recent history of eating raw cow liver, 87.5% of seropositive patients had this history. Multivariate statistical analysis showed that a recent history of eating raw cow liver was related to an increased risk of toxocariasis. Collectively, it is proposed that raw cow liver is a significant infection source of toxocariasis in the patients with eosinophilia of unknown etiology.

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Seroprevalence of Toxocariasis among Healthy People with Eosinophilia
Yong-Hun Kim, Sun Huh, Young-Bae Chung
Korean J Parasitol 2008;46(1):29-32.
Published online March 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.1.29

The aim of this study is to determine the Toxocara seropositive rate among healthy people with eosinophilia. A total of 97 people residing in Seoul who were healthy and whose blood eosinophilia was over 10%, as shown by regular health check-ups in 2004, were subjected to this study. Their sera were tested by immunoblotting and ELISA with the antigen of larval Toxocara canis excretory-secretory (ES) protein. Sixty-five sera were band-positive (67.0%). The seropositve control sera were positive to band sizes of 66 kDa, 56 kDa, 32 kDa, and 13 kDa. In ELISA, 63 sera (65.0%) were positive to T. canis ES protein. There was no significant correlation between the IgG ELISA titer and the level of eosinophilia (r = 0.156, P = 0.156). As there were insufficient data to determine whether there were cross-reactions with other helminthic infections, or whether atopy occurred, further studies are required to verify the cause of the seropositive reactions against T. canis ES antigen. Toxocariasis seropositivity is suggested to be the major cause of eosinophilia, since the Toxocara seroprevalence among Korean rural adults was shown to be approximately 5%.

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The phylogenic relationships existing among 14 parasitic Platyhelminthes in the Republic of Korea were investigated via the use of the partial 28S ribosomal DNA (rDNA) D1 region and the partial mitochondrial cytochrome c oxidase subunit 1 (mCOI) DNA sequences. The nucleotide sequences were analyzed by length, G + C %, nucleotide differences and gaps in order to determine the analyzed phylogenic relationships. The phylogenic patterns of the 28S rDNA D1 and mCOI regions were closely related within the same class and order as analyzed by the PAUP 4.0 program, with the exception of a few species. These findings indicate that the 28S rDNA gene sequence is more highly conserved than are the mCOI gene sequences. The 28S rDNA gene may prove useful in studies of the systematics and population genetic structures of parasitic Platyhelminthes.

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Brief Communication

Cloning of the novel putative apoptosis-related gene of Spirometra erinacei (Order Pseudophyllidea)
Soo-Ung Lee, Sun Huh
Korean J Parasitol 2006;44(3):233-237.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.233

We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.

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  • The genome of the sparganosis tapeworm Spirometra erinaceieuropaeiisolated from the biopsy of a migrating brain lesion
    Hayley M Bennett, Hoi Ping Mok, Effrossyni Gkrania-Klotsas, Isheng J Tsai, Eleanor J Stanley, Nagui M Antoun, Avril Coghlan, Bhavana Harsha, Alessandra Traini, Diogo M Ribeiro, Sascha Steinbiss, Sebastian B Lucas, Kieren SJ Allinson, Stephen J Price, Thom
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Mini Review

A successful experience of soil-transmitted helminth control in the Republic of Korea
Sung-Tae Hong, Jong-Yil Chai, Min-Ho Choi, Sun Huh, Han-Jong Rim, Soon-Hyung Lee
Korean J Parasitol 2006;44(3):177-185.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.177

Soil-transmitted helminths (STH), namely Ascaris, Trichuris and hookworms (Ancylostoma and Necator), present a global health problem to about a half of the earth's population. In the Republic of Korea, STH were highly prevalent and were considered a high priority target for national control. To promote the control, a non-governmental organization named Korea Association for Parasite Eradication (currently Korea Association of Health Promotion) was founded in 1964, and mass fecal examination followed by selective mass chemotherapy with anthelmintics was performed twice a year from 1969 to 1995 targeting whole nationwide schoolchildren. Meanwhile, decreasing patterns of national STH infections have been monitored by 7 times' quinquennial national surveys targeting general population. In 1971, the overall intestinal helminth egg positive rate was 84.3% (Ascaris 58.2%, Trichuris 65.4%, and hookworms 10.7%), which became 63.2% in 1976, 41.1% in 1981, 12.9% in 1986, 3.8% in 1992, 2.4% in 1997, and 4.3% (Ascaris 0.03%, Trichuris 0.02%, and hookworms 0%) in 2004. During the control period, national economy rapidly developed, and living standards including environment, sanitation, and agricultural technology greatly improved, which undoubtedly boosted the STH control effects. Our experience indicates that social driving force to establish an eligible national control system to conduct repeated mass chemotherapy, together with improvement of environment and sanitation, is important for initiating and achieving STH control in a developing community.

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Brief Communications

Intestinal protozoan infections and echinococcosis in the inhabitants of Dornod and Selenge, Mongolia (2003)
Sun Huh, Jae-Ran Yu, Jong-Il Kim, Choijamts Gotov, Radnaabazar Janchiv, Jeong-Sun Seo
Korean J Parasitol 2006;44(2):171-174.
Published online June 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.2.171

The status of intestinal parasitic infections and seroprevalence of cystic echinococcosis in the inhabitants in Dornod and Selenge, Mongolia, was observed with stool and serum samples from 165 and 683 residents from August 9 to August 15, 2003. A total of 10 inhabitants (6.1%) were found to be infected with protozoan cysts or oocysts by stool examinations; 7, 1 and 2 cases with Entamoeba coli, Giardia lamblia and Cryptosporidium parvum, respectively. A total of 62 people (9.1%) revealed significantly high levels of specific antibodies against Echinococcus granulosus by enzyme-linked immunosorbent assay (ELISA). The surveyed areas are rural areas and the sanitized tab water is not available. There is a possibility of endemic transmission of water-borne protozoan diseases in these areas. The clinical echinococcosis is needed to be searched from the seropositive inhabitants.

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The intestines and hearts of dogs were examined for Toxocara canis, Toxascaris leonina, and Dirofilaria immitis, after necropsy between June 26 and September 29, 2004 in Chuncheon, Korea. Of the 662 dogs examined, 6 were infected with T. canis (0.9%), 86 with T. leonina (13.0%). Fifty dogs were infected with D. immitis among 500 dogs examined (10.0%). Five were co-infected with T. canis and T. leonina, and three were co-infected with T. leonina and D. immitis. The cumulative positive infection rate for three species was 134/662(20.2%). Considering previously reported seropositive rates of T. canis excretory-secretory antigen, i.e., 5% in the adult population in Korea, the possibility of toxocariasis caused by T. leonina should be reevaluated.

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Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis
Soo-Ung Lee, Sun Huh
Korean J Parasitol 2004;42(3):145-148.
Published online September 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.3.145

We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.

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Original Article

Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
Korean J Parasitol 2004;42(3):129-135.
Published online September 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.3.129

We compared the DNA sequences of the genus Metagonimus: M. yokogawai, M. takahashii, and M. miyatai. We obtained 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome c oxidase subunit I (mtCOI) fragments from the adult worms by PCR, that were cloned and sequenced. Phylogenetic relationships inferred from the nucleotide sequences of the 28S D1 rDNA and mtCOI gene. M. takahashii and M. yokogawai are placed in the same clade supported by DNA sequence and phylogenic tree analysis in 28S D1 rDNA and mtCOI gene region. The above findings tell us that M. takahashii is closer to M. yokogawai than to M. miyatai genetically. This phylogenetic data also support the nomination of M. miyatai as a separate species.

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Brief Communication

To determine the molecular phylogenic location of Plagiorchis muris, 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome C oxidase subunit I (mtCOI) were sequenced and compared with other trematodes in the family Plagiorchiidae. The 28S D1 tree of P. muris was found to be closely related to those of P. elegans and other Plagiorchis species. And, the mtCOI tree also showed that P. muris is in a separate clade with genus Glypthelmins. These results support a phylogenic relationship between members of the Plagiorchiidae, as suggested by morphologic features.

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Original Article

Prevalence of cryptosporidiosis among the villagers and domestic animals in several rural areas of Korea
Jae-Ran Yu, Jong-Kyu Lee, Min Seo, Seok-Il Kim, Woon-Mok Sohn, Sun Huh, Hae-Yeon Choi, Tong-Soo Kim
Korean J Parasitol 2004;42(1):1-6.
Published online March 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.1.1

The present study was undertaken to investigate the infection status of Cryptosporidium parvum in the villagers and the reservoir hosts in several rural areas in Korea. A total 5,262 fecal samples were collected from the inhabitants residing at Gangwon-do, Chungcheongbuk-do, Jeollanam-do, and Gyeongsangnam-do between the dates of September, 2001 to June, 2002. In addition, 1,453 fecal samples were collected from livestock reared in Gokseong-gun, Jeollanam-do and Chungju-si, Chungcheongbuk-do. All the fecal smears were prepared by formalin-ether sedimentation, and examined by light microscopy after modified acid-fast staining. The overall positive rate of human cryptosporidiosis was 3.3%. Gokseong-gun, Jeollanam-do showed a 8.2% positive rate and appeared as the highest endemic area among the surveyed areas. Haman-gun, Gyeongsangnam-do showed a 0.4% positive rate and was the lowest endemic area. The positive rate of livestock infection in Gokseong-gun, Jeollanam-do was 94%, which was more than ten times higher than that of Chungju-si, Chungcheongbuk-do (9.3%). From these results, it was revealed that cryptosporidiosis was an endemic disease in some rural areas of Korea, and the livestock could be an important source of human infection.

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  • Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea
    Sang-Mee Guk, Tai-Soon Yong, Soon-Jung Park, Jae-Hwan Park, Jong-Yil Chai
    The Korean Journal of Parasitology.2004; 42(2): 85.     CrossRef
  • Parasitic Diseases in Children
    Sun Huh
    Journal of the Korean Medical Association.2004; 47(6): 512.     CrossRef
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Brief Communication

Molecular identification of Korean Trichinella isolates
Woon-Mok Sohn, Sun Huh, Dong-Il Chung, Edoardo Pozio
Korean J Parasitol 2003;41(2):125-127.
Published online June 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.2.125

Muscle larvae of Trichinella isolates from two outbreaks in Korea were analyzed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiple-PCR. All of the muscle larvae showed a band similar to that of T. spiralis larvae of the reference strain. The two Korean Trichinella isolates (isolate code ISS623 and ISS1078) might be classifiable to Trichinella spiralis.

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Original Article

A seroepidemiological survey for toxocariasis in apparently healthy residents in Gangwon-do, Korea
Hyun-Young Park, Soo-Ung Lee, Sun Huh, Yoon Kong, Jean-Fran?ois Magnaval
Korean J Parasitol 2002;40(3):113-117.
Published online September 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.3.113

We investigated the sero-prevalence of toxocariasis among healthy Korean adults in 1999. A total of 314 sera from normal inhabitants in Whachon-gun, Gangwon-do, Korea was examined for specific antibody levels against excretory-secretory products of second stage larvae of Toxocara (TES). The presence of cross-reactions with other helminthiases such as cysticercosis, paragonimiasis, sparganosis or clonorchiasis was also checked by specific IgG ELISA. Sera showing positive reaction against TES were also tested by IgG immunoblot and by IgE ELISA. Out of 314 subjects, 16 was found to be positive by TES IgG ELISA and immunoblot, among whom 12 were also positive by TES IgE ELISA. Among the 16 seropositive samples, two sera showed positive reaction against Paragonimus and sparganum antigen, respectively. These results inferred that cross-reactions were negligible between toxocariasis and other helminthiases. Toxocariasis seroprevalence among Korean rural adults was detected to be approximately 5%.

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  • Significance of Serum Antibody Test for Toxocariasis in Healthy Healthcare Examinees with Eosinophilia in Seoul and Gyeongsangnam-do, Korea
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  • Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae
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Brief Communications

Enzooticity of the dogs, the reservoir host of Thelazia callipaeda, in Korea
Min Seo, Jae-Ran Yu, Hyun-Young Park, Sun Huh, Sang-Kun Kim, Sung-Tae Hong
Korean J Parasitol 2002;40(2):101-103.
Published online June 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.2.101

The reservoir hosts of Thelazia callipaeda were examined. The eyes of the 76 dogs raised at farm, 78 military dogs (shepherds), 96 cattle, and 105 pigs were investigated for the presence of eyeworm. Among them, six worms of T. callipaeda were collected from two dogs raised at farm (2.7%), and 188 worms from 26 shepherds (33.5%). No worms were recovered from the cattle or pigs. These results suggest that the dogs, especially the military dogs are serving as a reservoir host of T. callipaeda in Korea.

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  • Thelazia Callipaeda Infestation with Tarsal Ectropion
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An epidemiological survey on Cryptosporidium parvum infection of inhabitants in Chorwon-gun, Kangwon-do
Min Seo, Sun Huh, Jong-Yil Chai, Jae-Ran Yu
Korean J Parasitol 2001;39(2):201-203.
Published online June 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.2.201

The present study was undertaken to know the infection status of Cryptosporidium parvum among the residents of Chorwon-gun, Kangwon-do in 1993. Total 461 fecal samples were collected from the inhabitants residing in Chorwon-gun during the period of August 12 to September 14, 1993. Fecal smears were prepared by formalin-ether sedimentation, and examined after modified acid fast staining. Of the 461 fecal samples, 9 (1.9%) were positive for C. parvum oocysts. The positive cases were limited to thirties (4) patients, forties (3), and sixties (2), and no oocyst was detected in other age groups. The oocyst positive rate for male was 1.4% and that of female was 2.6%.

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    Mohammad Mirzaei
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    The Korean Journal of Parasitology.2004; 42(1): 1.     CrossRef
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Enterobius vermicularis egg positive rate of pre-school children in Chunchon, Korea (1999)
Hyung-Jun Yoon, Ye-Jong Choi, Soo-Ung Lee, Hyun-Young Park, Sun Huh, Yong-Sang Yang
Korean J Parasitol 2000;38(4):279-281.
Published online December 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.4.279

Pre-school children were examined for the presence of Enterobius vermicularis egg by perianal swab method. We visited 67 nurseries and 25 kindergartens between January, 1999 and April, 1999 in Chunchon, Korea. Of the 4,711 children examined, 434 (9.2%) were found to be positive for E. vermicularis egg. The egg positive rates of boys and girls were 10.1% and 8.1%, respectively. The rates were 7.8% in kindergartens and 9.7% in nurseries. Positive rate of 50 institutions was less than 10%. Rate in 35 institutions was from 10% to less than 20%. Rate in 7 institutions was equal to or over 20%. Out of 1,113 children examined twice, 28 (2.5%) children were positive consecutively, 53 (4.8%) were positive with negative conversion, 47 (4.2%) were negative with positive conversion, and 985 (88.5%) were consecutively negative. It means that in the low endemic area of enterobiasis with around 10% positive rate, the two consecutive examinations may increase the egg detection rate of 4.2-4.8%. The small number of consecutive egg positive children also suggests that the worm burden of the positive children might be low. Since the egg positive rate of that age group in this City increased from 1.85% (1997), and 3.0% (1998), the more intensive regular control should be executed.

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    Stavros Panidis, Daniel Paramythiotis, Dimitris Panagiotou, Georgios Batsis, Spyridon Salonikidis, Vassiliki Kaloutsi, Antonios Michalopoulos
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    Mare Remm, Kalle Remm
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    Betina C Pezzani
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    Bong Jin Kim, Bo Young Lee, Hyun Kee Chung, Young Sun Lee, Kun Hee Lee, Hae Jin Chung, Mee Sun Ock
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  • Enterobius vermicularis egg positive rates in primary school children in Gangwon-do (Province), Korea
    Kyu-Jae Lee, Yung-Kyum Ahn, Yong-Suk Ryang
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  • Infection rates of Enterobius vermtcularis and Clonorchis sinensis of primary school children in Hamyang-gun, Gyeongsangnam-do (Province), Korea
    Bong Jin Kim, Je Wook Yeon, Mee Sun Ock
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Case Report

Five cases of ocular toxocariasis confirmed by serology
Sung-Pyo Park, Inwon Park, Hyun-Young Park, Soo-Ung Lee, Sun Huh, Jean-Fran?ois Magnaval
Korean J Parasitol 2000;38(4):267-273.
Published online December 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.4.267

We report 5 cases of ocular toxocariasis in Korean adults complaining of visual impairment along with floating or bubbling sensation. Fundoscopic examination revealed a retinal detachment along with exudate in 4 cases. They all showed typical reaction by ELISA and immunoblot against Toxocra excretory-secretory antigen. One case showed high level of anti-Toxocara IgE antibodies (34,000 Toxocara units/L) as well as increased level of serum total IgE antibodies and the specific IgE antibodies for 3 inhalant antigens, suggesting that high level of anti-Toxocara IgE antibodies was associated with an atopic status. Clinical manifestations were improved after the sequential use of steroids then mebendazole. We also suggest that ocular toxocariasis should be thoroughly investigated even when an evocative uniocular inflammatory lesion is encountered in peripheral retina without a systematic disease.

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Original Articles

Chromosomes of the liver fluke, Clonorchis sinensis
Gab-Man Park, Kyung-il Im, Sun Huh, Tai-Soon Yong
Korean J Parasitol 2000;38(3):201-206.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.201

A karyological study was carried out in order to compared the chromosome numbers, chromosome morphologies and karyotypes of the oriental liver fluke, Clonorchis sinensis (Trematoda: Opisthorchiidae), collected from Korea and China. Chromosome preparations were made by means of air-drying method. The chromosome number was 2n=56 in both Korean and Chinese flukes, and chromosomes were divided into two groups based on this size; consisting of 8 pairs of large and 20 pairs of small chromosomes. However, the karyotypes showed some differences between Korean and Chinese flukes. The karyotype of liver flukes from Korea consisted of three metacentric pairs, one meta-/submetacentric pair, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes. On the other hand, liver flukes from China consisted of two metacentric pairs, two meta-/submetacentric pairs, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes.

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    Maria Y. Pakharukova, Viatcheslav A. Mordvinov
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  • High-quality reference genome for Clonorchis sinensis
    Neil D. Young, Andreas J. Stroehlein, Liina Kinkar, Tao Wang, Woon-Mok Sohn, Bill C.H. Chang, Parwinder Kaur, David Weisz, Olga Dudchenko, Erez Lieberman Aiden, Pasi K. Korhonen, Robin B. Gasser
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  • Genomics of worms, with an emphasis on Opisthorchis viverrini — opportunities for fundamental discovery and biomedical outcomes
    Robin B. Gasser, Patrick Tan, Bin Tean Teh, Sopit Wongkham, Neil D. Young
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    Marta Bombarová, Marta Špakulová, Martin Kello, Petr Nguyen, Eva Bazsalovicsová, Ivica Králová-Hromadová
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    Neil D. Young, Niranjan Nagarajan, Suling Joyce Lin, Pasi K. Korhonen, Aaron R. Jex, Ross S. Hall, Helena Safavi-Hemami, Worasak Kaewkong, Denis Bertrand, Song Gao, Qihui Seet, Sopit Wongkham, Bin Tean Teh, Chaisiri Wongkham, Pewpan Maleewong Intapan, Wan
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    Jiufeng Sun, Yan Huang, Huaiqiu Huang, Pei Liang, Xiaoyun Wang, Qiang Mao, Jingtao Men, Wenjun Chen, Chuanhuan Deng, Chenhui Zhou, Xiaoli Lv, Juanjuan Zhou, Fan Zhang, Ran Li, Yanli Tian, Huali Lei, Chi Liang, Xuchu Hu, Jin Xu, Xuerong Li, XinbingYu, Dav
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  • Genomics and molecular genetics of Clonorchis sinensis: Current status and perspectives
    Si-Yang Huang, Guang-Hui Zhao, Bao-Quan Fu, Min-Jun Xu, Chun-Ren Wang, Song-Ming Wu, Feng-Cai Zou, Xing-Quan Zhu
    Parasitology International.2012; 61(1): 71.     CrossRef
  • Clarification of the systematic position ofCercariaeum crassumWesenberg-Lund, 1934 (Digenea), based on karyological analysis and DNA sequences
    R. Petkevičiūtė, V. Stunžėnas, G. Stanevičiūtė
    Journal of Helminthology.2012; 86(3): 293.     CrossRef
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    Kira S. Zadesenets, Alexei V. Katokhin, Viatcheslav A. Mordvinov, Nikolay B. Rubtsov
    Parasitology International.2012; 61(1): 87.     CrossRef
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    Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
    The Korean Journal of Parasitology.2009; 47(Suppl): S59.     CrossRef
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    De-Hua Lai, Qiao-Ping Wang, Wen Chen, Lian-Shun Cai, Zhong-Dao Wu, Xing-Quan Zhu, Zhao-Rong Lun
    Acta Tropica.2008; 107(2): 213.     CrossRef
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    Gab-Man Park
    Parasitology Research.2007; 100(2): 351.     CrossRef
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    Thewarach Laha, Porntip Pinlaor, Jason Mulvenna, Banchob Sripa, Manop Sripa, Michael J Smout, Robin B Gasser, Paul J Brindley, Alex Loukas
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    H.-J. Rim
    Journal of Helminthology.2005; 79(3): 269.     CrossRef
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    Gab-Man Park, Tai-Soon Yong
    The Korean Journal of Parasitology.2003; 41(1): 41.     CrossRef
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Genetic heterogeneity of Pneumocystis carinii from rats of several regions and strains
Byung-Suk Chung, Yun-Kyu Pars, Sun Huh, Jae-Ran Yu, Jin Kim, Xiaohua Shi, Sang Rock Cho, Soon-Hyung Lee, Sung-Tae Hong
Korean J Parasitol 2000;38(3):151-158.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.151

Pneumocystis carinii is a major opportunistic pathogen which has been found in the lungs of a wide variety of mammalian host species, and the fact suggests the possibility of intraspecific variation. Until now, P. carinii from different mammalian species are differentiated as subspecies, and the rats are known to be infected by two subspecies. The present study investigated genetic heterogeneity of P. carinii isolates from two strains of rats in Korea and China by molecular karyotyping, RFLP and sequencing analysis. Karyotypes of P. carinii were grouped into three, two from two strains of rats in Korea and one from rats in China. However RFLP of PCR product of ribosomal and MSG gene of the P. carinii isolates showed same pattern. The sequence homology rates of α-tubulin DNA of the P. carinii isolates were 96% in Seoul Wistar rats, 93% in Seoul Sprague-Dawley rats, and 85% in Chinese Sprague-Dawley rats. The present finding confirmed that P. carinii from rats in Korea are grouped into two karyotype strains which are different from that of P. carinii from rats in China. The Chinese isolate shows a little different sequences of α-tubulin DNA.

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    Belén Budiño, Jesús Lamas, María P. Pata, Juan A. Arranz, Manuel L. Sanmartín, José Leiro
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    Jae-Seok Kim, Yong-Kyun Kim, Ji Young Park, Eun Kyung Mo, Han Sung Kim, Wonkeun Song, Hyoun Chan Cho, Kyu Man Lee
    Chonnam Medical Journal.2008; 44(2): 82.     CrossRef
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A cytogenetic study on human intestinal trematodes of the genus Metagonimus (Digenea: Heterophyidae) in Korea
Soo-Ung Lee, Sun Huh, Gab-Man Park, Jong-Yil Chai
Korean J Parasitol 1999;37(4):237-241.
Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.237

In order to analyze chromosome numbers and karyotypes of intestinal trematodes belonging to the genus, Metagonimus, the gonad tissues of M. takahashii, M. miyatai, and M. yokogawai were prepared and examined. The number of bivalents in the first meiotic division of M. takahashii was nine (n=9). The diploid number of M. miyatai was observed to be eighteen (2n=18) and their chromosomes consisted of one pair of metacentric, 7 pairs of submetacentric, and one pair of telocentric chromosomes. The diploid number of M. yokogawai was thirty-two (2n=32) and the chromosome complements were composed of two pairs of metacentric, 11 pairs of submetacentric, and three pairs of subtelocentric chromosomes. These results could be a supporting evidence for the validity of the new species, M. miyatai, distinct from M. yokogawai

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    Jong-Yil Chai, Bong-Kwang Jung
    Food and Waterborne Parasitology.2017; 8-9: 33.     CrossRef
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    L. Rafat-Motavalli, H. Miri-Hakimabad, E. Bakhtiyari
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  • Foodborne Intestinal Flukes in Southeast Asia
    Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • SUSCEPTIBILITY OF INBRED MOUSE STRAINS TO INFECTION WITH THREE SPECIES OF METAGONIMUS PREVALENT IN THE REPUBLIC OF KOREA
    Sang-Mee Guk, Jin-Young Park, Min Seo, Eun-Taek Han, Jae-Lip Kim, Jong-Yil Chai
    Journal of Parasitology.2005; 91(1): 12.     CrossRef
  • Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
    Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
    The Korean Journal of Parasitology.2004; 42(3): 129.     CrossRef
  • Food-borne intestinal trematode infections in the Republic of Korea
    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
  • 10,135 View
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Infection status of dragonflies with Plagiorchis muris metacercariae in Korea
Sung-Jong Hong, Ho-Chun Woo, Soo-Ung Lee, Sun Huh
Korean J Parasitol 1999;37(2):65-70.
Published online June 30, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.2.65

Plagiorchis muris has been found in both house and field rats as well as in humans. The infection status of the second intermediate hosts of P. muris is prerequisite in understanding their biological features in an ecosystem. Six species of dragonflies were caught in a wide range of areas in Korea; and they were Sympetrum darwinianum, S. eroticum, S. pedomontanum, S. infuscatum, Pantala flavoscens, Calopteryx atrata, and Orthetrum albistylum speciosum. The occurrence of P. muris metacercariae in dragonflies was nationwide with various infection rates. The metacercarial burden of P. muris in the surveyed areas was the highest in S. eroticum followed by S. darwinianum, S. pedomontanum, and C. atrata. The highest infection rate by P. muris metacercariae was found in S. darwinianum followed by S. eroticum. The metacercarial burden was particularly heavy in the dragonflies found in Hamyang-gun and Kosong-gun, Kyongsangnam-do. It is, therefore, likely that dragonflies play a significant role as the second intermediate host in the life cycle of P. muris in Korea.

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  • Survey of the Prosthogonimus spp. metacercariae infection in the second intermediate host dragonfly in Heilongjiang Province, China
    Ben Li, Zhuo Lan, Xin-Ru Guo, Ai-Hui Zhang, Wei Wei, Ye Li, Zhen-Hua Jin, Zhong-Yan Gao, Xian-Guang Zhang, Bai Li, Jun-Feng Gao, Chun-Ren Wang
    Parasitology Research.2023; 122(12): 2859.     CrossRef
  • Odonata (Insecta) Larvae as the Second Intermediate Hosts of the Trematodes of Genus Plagiorchis in the Basin of Chany Lake, Western Siberia
    N. M. Ponomareva, O. N. Popova, N. I. Yurlova
    Contemporary Problems of Ecology.2022; 15(6): 631.     CrossRef
  • Influences of habitat and arthropod density on parasitism in two co-occurring host taxa
    J. Koprivnikar, T.M.Y. Urichuk, D. Szuroczki
    Canadian Journal of Zoology.2017; 95(8): 589.     CrossRef
  • A molecular and ecological analysis of the trematodePlagiorchis elegansin the wood mouseApodemus sylvaticusfrom a periaquatic ecosystem in the UK
    K. Boyce, G. Hide, P.S. Craig, C. Reynolds, M. Hussain, A.J. Bodell, H. Bradshaw, A. Pickles, M.T. Rogan
    Journal of Helminthology.2014; 88(3): 310.     CrossRef
  • Trematodes and nematodes parasitizing the benthic insect community of an Andean Patagonian stream, with emphasis on plagiorchiid metacercariae
    Gustavo D. Mariluan, Gustavo P. Viozzi, Ricardo J. Albariño
    Invertebrate Biology.2012; 131(4): 285.     CrossRef
  • Plagiorchis muris infection in Apodemus agrarius from northern Gyeonggi-do (Province) near the demilitarized zone
    Jong-Yil Chai, Jae-Hwan Park, Sang-Mee Guk, Jae-Lip Kim, Hyo-Jin Kim, Won-Hee Kim, Eun-Hee Shin, Terry A. Klein, Heung-Chul Kim, Sung-Tae Chong, Jin-Won Song, Luck-Ju Baek
    The Korean Journal of Parasitology.2007; 45(2): 153.     CrossRef
  • The occurrence of the trematodePlagiorchis murisin the wood mouseApodemus sylvaticusin North Yorkshire, UK
    M.T. Rogan, P.S. Craig, G. Hide, S. Heath, A. Pickles, D.M. Storey
    Journal of Helminthology.2007; 81(1): 57.     CrossRef
  • A Human Case of Plagiorchis vespertilionis (Digenea: Plagiorchiidae) Infection in the Republic of Korea
    S-M. Guk, J-L. Kim, J-H. Park, J-Y. Chai
    Journal of Parasitology.2007; 93(5): 1225.     CrossRef
  • Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I
    Soo-Ung Lee, Sun Huh, Woon-Mok Sohn
    The Korean Journal of Parasitology.2004; 42(2): 71.     CrossRef
  • Food-borne intestinal trematode infections in the Republic of Korea
    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
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  • 69 Download
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Variation of antigenicity and serological reaction to Pneumocystis carinii in Korea
Hyun-Young Park, Soo-Ung Lee, Seoung-Wan Chae, Sun Huh, Jae-Ran Yu, Jin Kim, Sung-Tae Hong
Korean J Parasitol 1999;37(2):109-115.
Published online June 30, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.2.109

The present study observed the variation of antigenicity of Pneumocystis carinii and serum IgG antibody reaction to the antigens from different localities in Korea. Antigens of rat P. carinii and sera of inhabitants were collected at Chunchon, Chungju, Kwangju, and Seoul during 1995-1996. Enzyme-Linked Immunosorbent Assay and immunoblot were used for immune reaction. Absorbance of 1,294 human sera ranged between 0.01 and 0.93. Sera from Chunchon showed higher absorbances than those from other areas. Immunoblotting revealed IgG antibody reactions to 116, 100, and 45-55 kDa antigenic bands of rat P. carinii, but the frequencies of positive reaction to individual bands were variable by localities. Total 62.6% of the sera showed the reaction to 116 kDa band while 37.7% reacted to 100 kDa band and 32.0% to 45-55 kDa bands. For the reaction to 116 kDa, the reaction rate was 60.0% to 82.6% by localities. It is found that the reaction rates of the human sera to rat P. carinii antigen are variable according to the localities. Also, the high molecular antigen of 116 kDa of rat P. carinii is the most frequent antigenic band reacting to human sera.

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  • Human Immunodeficiency Virus-Infected Patients with PriorPneumocystisPneumonia Exhibit Increased Serologic Reactivity to Several Major Surface Glycoprotein Clones
    K. R. Daly, J. V. Koch, N. J. Shire, L. Levin, P. D. Walzer
    Clinical and Vaccine Immunology.2006; 13(10): 1071.     CrossRef
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  • 61 Download
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Case Reports

An aural myiasis case in a 54-year-old male farmer in Korea
Jin-Hak Cho, Hyung-Baek Kim, Chin-Saeng Cho, Sun Huh, Han-Il Ree
Korean J Parasitol 1999;37(1):51-53.
Published online March 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.1.51

A 54-year-old male farmer residing in Chunchon, Korea, complaining of blood tinged discharge and tinnitus in the left ear for two days, was examined in August 16, 1996. Otoscopic examination revealed live maggots from the ear canal. The patient did not complain of any symptoms after removal of maggots. Five maggots recovered were identified as the third stage larvae of Lucilia sericata (Diptera: Calliphoridae). This is the first record of aural myiasis in Korea.

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  • AURAL MYIASIS, AN UNUSUAL PRESENTATION
    Anupam Batra, Anita Singhare, Vishal R. Munjal
    PARIPEX INDIAN JOURNAL OF RESEARCH.2021; : 73.     CrossRef
  • Aural Myiasis: A Case Report on a Rare Entity
    Ellen Rummens, Gerry Van der Mieren, Vincent Van Rompaey, Peter Piessens, Francis Somville
    Cureus.2020;[Epub]     CrossRef
  • A clinico-etiological study of aural myiasis
    Pradip Mallik, Vikas Sinha, Sushil Jha, Jigna Swani, Naimish Maniya, Sandeep Yadav
    Indian Journal of Otology.2019; 25(4): 180.     CrossRef
  • Canine Wound Myiasis Caused by Lucilia sericata (Diptera: Calliphoridae) in Korea
    Seongjun Choe, Dongmin Lee, Hansol Park, Hyeong-Kyu Jeon, Hakhyun Kim, Ji-Houn Kang, Cha-Ho Jee, Keeseon S. Eom
    The Korean Journal of Parasitology.2016; 54(5): 667.     CrossRef
  • A Case of Aural Myiasis in Chronic Alcoholism Patient
    Sung Hoon Jung, Jae Hwan Jung, Seok Hyun Kim, Yang Jae Kim
    Journal of Clinical Otolaryngology Head and Neck Surgery.2016; 27(2): 332.     CrossRef
  • Incidence of myiasis among humans—a review
    Amandeep Singh, Zorawar Singh
    Parasitology Research.2015; 114(9): 3183.     CrossRef
  • Aural Myiasis, a Rare Cause of Earache
    Ibrahim Al Jabr
    Case Reports in Otolaryngology.2015; 2015: 1.     CrossRef
  • Myiasis of the Ear
    Joshua Jervis-Bardy, Nick Fitzpatrick, Ajmal Masood, Graeme Crossland, Hemi Patel
    Annals of Otology, Rhinology & Laryngology.2015; 124(5): 345.     CrossRef
  • A Case of Nosocomial Nasal Myiasis in Comatose Patient
    Sung Jae Heo, Mi Jin Lee, Chang Mook Park, Jung Soo Kim
    Korean Journal of Otorhinolaryngology-Head and Neck Surgery.2013; 56(10): 664.     CrossRef
  • A Case of Oral Myiasis Caused by Lucilia sericata (Diptera: Calliphoridae) in Korea
    Mun Jang, Seung-Min Ryu, Sang-Chang Kwon, Jun-Ouk Ha, Young-Hoon Kim, Dong-Hyun Kim, Soon-Myung Jung, Soon-Il Lee, Woon-Mok Sohn, Hee-Jae Cha, Meesun Ock
    The Korean Journal of Parasitology.2013; 51(1): 119.     CrossRef
  • Myiasis
    Fabio Francesconi, Omar Lupi
    Clinical Microbiology Reviews.2012; 25(1): 79.     CrossRef
  • A Nasal Myiasis in a 76-Year-Old Female in Korea
    Jae-Soo Kim, Pil-Won Seo, Jong-Wan Kim, Jai-Hyang Go, Soon-Cheol Jang, Hye-Jung Lee, Min Seo
    The Korean Journal of Parasitology.2009; 47(4): 405.     CrossRef
  • The insecticidal activity of four medicinal plants against the blowfly Lucilia sericata (Diptera: Calliphoridae)
    Hanem Fathy Khater, D. F. Khater
    International Journal of Dermatology.2009; 48(5): 492.     CrossRef
  • Patient With Persistent Wound Drainage
    Amit Agrawal, S.R. Joharapurkar, Abhishek Wadaskar, Bhushan Wani, Jitesh Khandarani
    Infectious Diseases in Clinical Practice.2009; 17(4): 258.     CrossRef
  • Aural myiasis in a patient with Alzheimer’s disease
    Pascal Werminghaus, Thomas K. Hoffmann, Heinz Mehlhorn, Murat Bas
    European Archives of Oto-Rhino-Laryngology.2008; 265(7): 851.     CrossRef
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    Süleyman Yazar, Bilal Dik, Şaban Yalçın, Funda Demirtaş, Ozan Yaman, Mustafa Öztürk, İzzet Şahin
    Yonsei Medical Journal.2005; 46(3): 431.     CrossRef
  • Aural Myiasis in Children and Literature Review
    Köksal Yuca, Hüseyin Çaksen, Yunus Feyyat Sakin, Sevil Ari Yuca, Muzaffer Kiris, Hasan Yilmaz, Hakan Çankaya
    The Tohoku Journal of Experimental Medicine.2005; 206(2): 125.     CrossRef
  • Aural live foreign bodies in children
    Köksal Yuca, Sevil Arı Yuca, Hüseyin Çaksen
    The Journal of Emergency Medicine.2003; 25(1): 102.     CrossRef
  • Cutaneous myiasis caused by Lucilia sericata (Diptera : Calliphoridae) on skin cancer of the cheek
    Yuichi CHIGUSA, Satoshi SHINONAGA, Tatsuhito KAWAGUCHI, Naoko KAWAGUCHI, Masashi KIRINOKI, Hajime MATSUDA
    Medical Entomology and Zoology.2002; 53(2): 89.     CrossRef
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A case of gastric pseudoterranoviasis in a 43-year-old man in Korea
Moon-Soo Koh, Sun Huh, Woon-Mok Sohn
Korean J Parasitol 1999;37(1):47-49.
Published online March 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.1.47

A case of Pseudoterranova decipiens infection was found in a 43-year-old man by gastroendoscopic examination on August 20, 1996. On August 6, 1996, he visited a local clinic, complaining of epigastric pain two days after eating raw marine fishes. Although the symptoms were relieved soon, endoscopic examination was done for differential diagnosis. A white, live nematode larva was removed from the fundus of the stomach. The larva was 38. 3×1.0 mm in size and had a cecum reaching to the mid-level of the ventriculus. A lot of transverse striations were regularly arranged on the cuticle of its body surface, but the boring tooth and mucron were not observed at both ends of the worm. The worm was identified as the 4th stage larva of P. decipiens.

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  • Gastrointestinal parasites of harbour seal (Phoca vitulina L.) in Danish marine waters: Prevalence, abundance, intensity and reproductive potential
    Kaan Kumas, Carlota Marola Fernandez Gonzalez, Per Walter Kania, Kurt Buchmann
    International Journal for Parasitology: Parasites and Wildlife.2025; 27: 101066.     CrossRef
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    Kurt Buchmann
    Folia Parasitologica.2023;[Epub]     CrossRef
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    A. Nordholm, J.A.L. Kurtzhals, A.M. Karami, P.W. Kania, K. Buchmann
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Effects of anisakid nematodes Anisakis simplex (s.l.), Pseudoterranova decipiens (s.l.) and Contracaecum osculatum (s.l.) on fish and consumer health
    Kurt Buchmann, Foojan Mehrdana
    Food and Waterborne Parasitology.2016; 4: 13.     CrossRef
  • Mitochondrial genomes of Anisakis simplex and Contracaecum osculatum (sensu stricto) – Comparisons with selected nematodes
    Namitha Mohandas, Abdul Jabbar, Magdalena Podolska, Xing-Quan Zhu, D. Timothy J. Littlewood, Aaron R. Jex, Robin B. Gasser
    Infection, Genetics and Evolution.2014; 21: 452.     CrossRef
  • A human case of gastric infection by Pseudoterranova decipiens larva
    Jae-Ran Yu, Min Seo, Young-Wook Kim, Mee-Hee Oh, Woon-Mok Sohn
    The Korean Journal of Parasitology.2001; 39(2): 193.     CrossRef
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  • 72 Download
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Brief Communication
Karyotype analysis of Neodiplostomum seoulense
Gab-Man Park, Soo-Ung Lee, Hyun-Young Park, Sun Huh
Korean J Parasitol 1998;36(4):277-279.
Published online December 20, 1998
DOI: https://doi.org/10.3347/kjp.1998.36.4.277

A karyotype analysis of the chromosome of Neodiplostomum seoulense, one of causative agents of human intestinal trematodiasis, was done from the gonad tissue by the squashing method. The chromosome number was n = 10 and 2n = 20. Chromosome length was 1.30-4.0 ?m. Chromosome pairs in the complement consisted of two pairs of metacentric, five pairs of submetacentrics/subtelocentric and three pairs of telocentric chromosomes. These data were comparable with those of other intestinal trematodes.

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  • Occurrence of a Diploid Type and a New First Intermediate Host of a Human Lung Fluke, Paragonimus westermani, in Korea
    Gab Man Park, K.J. Lee, K.I. Im, H. Park, T.S. Yong
    Experimental Parasitology.2001; 99(4): 206.     CrossRef
  • Chromosomes of the liver fluke, Clonorchis sinensis
    Gab-Man Park, Kyung-il Im, Sun Huh, Tai-Soon Yong
    The Korean Journal of Parasitology.2000; 38(3): 201.     CrossRef
  • A cytogenetic study on human intestinal trematodes of the genus Metagonimus (Digenea: Heterophyidae) in Korea
    Soo-Ung Lee, Sun Huh, Gab-Man Park, Jong-Yil Chai
    The Korean Journal of Parasitology.1999; 37(4): 237.     CrossRef
  • 7,885 View
  • 118 Download
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