Parasites, Hosts and Diseases

"Teera Kusolsuk"

Case Report

Asymptomatic Human Paragonimiasis among the Karen People in Tak Province, Thailand: A Case Report: Teera Kusolsuk, Orawan Phuphisut, Wanna Maipanich, Somchit Pubampen, Surapol Sa-nguankiat, Akkarin Poodeepiyasawat, Nirundorn Homsuwan, Srisuchart Mongkolmoo, Tippayarat Yoonuan, Poom Adisakwattana, Udomsak Silachamroon, Yukifumi Nawa; Korean J Parasitol 2020;58(1):57-60.
Published online February 29, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.1.57

During the mobile clinic activities in Tak Province, Thailand, Paragonimus sp. eggs were found in a fecal sample of a 72-year-old Karen resident. Paragonimus DNA was amplified from the stool sample and identified to P. heterotremus. The patient did not have any symptoms. Apparent pulmonary lesion was not found on the chest X-ray. The patient admitted habitual consumption of semi-cooked or roasted waterfall crabs for several years. The waterfall crabs collected from stream near the village were found negative for Paragonimus metacercariae. In northern Thailand, paragonimiasis remains as one of the public health concerns and should be ruled out for asymptomatic pulmonary patients.

Citations

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Lung flukes of the genusParagonimus: ancient and re-emerging pathogens
David Blair
Parasitology.2022; 149(10): 1286. CrossRef

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Original Article

Subtype Distribution of Blastocystis in Thai-Myanmar Border, Thailand: Supaluk Popruk, Ruenruetai Udonsom, Khuanchai Koompapong, Aongart Mahittikorn, Teera Kusolsuk, Jiraporn Ruangsittichai, Attakorn Palasuwan; Korean J Parasitol 2015;53(1):13-19.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.13

Abstract
PDF

Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.

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Brief Communication

Egg Laying Capacity of Haplorchis taichui (Digenea: Heterophyidae) in Humans: Megumi Sato, Surapol Sanguankiat, Somchit Pubampen, Teera Kusolsuk, Wanna Maipanich, Jitra Waikagul; Korean J Parasitol 2009;47(3):315-318.
Published online August 28, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.3.315

Abstract
PDF

Quantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui (Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.

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13,303 View
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Case Report

Anchitrema sanguineum (Digenea: Anchitrematidae) Accidentally Found during Colonoscopy of a Patient with Chronic Abdominal Pain: A Case Report: Teera Kusolsuk, Nantana Paiboon, Somchit Pubampen, Wanna Maipanich, Paron Dekumyoy, Jitra Waikagul; Korean J Parasitol 2009;47(2):167-170.
Published online May 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.167

Abstract
PDF

In November 2007, a 46-year-old male Thai patient presented with chronic abdominal pain for over 3 years. Colonoscopy revealed a small parasite of about 2 × 1 mm in size attached to the cecum mucosa. The worm was removed endoscopically, fixed, and stained for morphological observations. The specimen was identified as Anchitrema sanguineum (Digenea: Anchitrematidae), a trematode first reported in a reptile, Chamaeleo vulgaris, from Egypt, and then sporadically found in the intestines of insectivorous bats and other mammals. The patient was treated with praziquantel but no more worms were found in his stool. His symptoms improved slightly but not cured completely. It remains unclear whether the chronic abdominal pain of the patient was caused by this trematode infection. Whatever is the pathogenicity of this trematode, this is the first human case of A. sanguineum infection in the literature.

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