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High Prevalence of Clonorchis sinensis and Other Zoonotic Trematode Metacercariae in Fish from a Local Market in Yen Bai Province, Northern Vietnam
Fuhong Dai, Sung-Jong Hong, Jhang Ho Pak, Thanh Hoa Le, Seung-Ho Choi, Byoung-Kuk Na, Woon-Mok Sohn
Korean J Parasitol 2020;58(3):333-338.
Published online June 26, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.3.333
A small survey was performed to investigate the recent infection status of Clonorchis sinensis and other zoonotic trematode metacercariae in freshwater fish from a local market of Yen Bai city, Yen Bai province, northern Vietnam. A total of 118 fish in 7 species were examined by the artificial digestion method on March 2016. The metacercariae of 4 species of zoonotic trematodes, i.e., C. sinensis, Haplorchis pumilio, Haplorchis taichui, and Centrocestus formosanus, were detected. The metacercariae of C. sinensis were found in 62 (69.7%) out of 89 fish (5 species), and their intensity of infection was very high, 81.2 per fish infected. Prevalences of 3 intestinal flukes, H. pumilio, H. taichui and C. formosanus, were 75.0%, 47.6%, and 31.7% in positive fish species, respectively, with the metacercarial intensities of 15.5, 10.3, and 2.2 per fish infected. From the above results, it has been confirmed that various species of freshwater fish continue to play the role of the infection source of C. sinensis and other zoonotic trematodes in Yen Bai city, Yen Bai province, northern Vietnam. It is of particular note that the prevalence and intensity of C. sinensis metacercariae are much higher than those reported in previous studies in fish in northern Vietnam.

Citations

Citations to this article as recorded by  Crossref logo
  • Hydropower reservoirs – their potential association with transmission of trematodes in Vietnam
    A.H. Le, H.V. Nguyen, H. Van Hoang, T.A. Tran, C.N. Nguyen, B.N. Nguyen, H.T. Nguyen, S.E. Greiman, J.R. Stauffer, H. Madsen, M.H. Nguyen
    Journal of Helminthology.2025;[Epub]     CrossRef
  • Infection with Clonorchis sinensis (Cobbold, 1875) Metacercariae in Fish from the East Lake of Wuhan: Freshwater Fish in Urban Lakes May Act as Infection Sources of Liver Fluke
    Jia-Nan Jiang, Hui-Fen Dong, Hou-Da Cheng, Hong Zou, Ming Li, Wen-Xiang Li, Gui-Tang Wang
    Microorganisms.2024; 12(5): 898.     CrossRef
  • An overview of human helminthioses in Vietnam: Their prevention, control and lessons learnt
    Hung Manh Nguyen, Dung Trung Do, Stephen E. Greiman, Ha Van Nguyen, Hien Van Hoang, Toan Quoc Phan, Phuc Pham-Duc, Henry Madsen
    Acta Tropica.2023; 238: 106753.     CrossRef
  • Parasite Diversity in a Freshwater Ecosystem
    Amana Shafiq, Farzana Abbas, Muhammad Hafeez-ur-Rehman, Bushra Nisar Khan, Ayesha Aihetasham, Iffat Amin, Hmidullah, Ramzi A. Mothana, Mohammed S. Alharbi, Imran Khan, Atif Ali Khan Khalil, Bashir Ahmad, Nimra Mubeen, Muneeba Akram
    Microorganisms.2023; 11(8): 1940.     CrossRef
  • A relatively high zoonotic trematode prevalence in Orientogalba ollula and the developmental characteristics of isolated trematodes by experimental infection in the animal model
    Jian Li, Yijing Ren, Lei Yang, Jiani Guo, Haiying Chen, Jiani Liu, Haoqiang Tian, Qingan Zhou, Weiyi Huang, Wei Hu, Xinyu Feng
    Infectious Diseases of Poverty.2022;[Epub]     CrossRef
  • Helminth infections in fish in Vietnam: A systematic review
    Trang Huyen Nguyen, Pierre Dorny, Thanh Thi Giang Nguyen, Veronique Dermauw
    International Journal for Parasitology: Parasites and Wildlife.2021; 14: 13.     CrossRef
  • Optimization of tools for the detection and identification of Cryptocotyle metacercariae in fish: Digestion method and viability studies
    Maureen Duflot, Graziella Midelet, Odile Bourgau, Kurt Buchmann, Mélanie Gay
    Journal of Fish Diseases.2021; 44(11): 1777.     CrossRef
  • Novel mechanism of hepatobiliary system damage and immunoglobulin G4 elevation caused by Clonorchis sinensis infection
    Xin-He Zhang, Die Huang, Yi-Ling Li, Bing Chang
    World Journal of Clinical Cases.2021; 9(23): 6639.     CrossRef
  • Current status of human liver fluke infections in the Greater Mekong Subregion
    Banchob Sripa, Apiporn T. Suwannatrai, Somphou Sayasone, Dung Trung Do, Virak Khieu, Yichao Yang
    Acta Tropica.2021; 224: 106133.     CrossRef
  • 6,614 View
  • 144 Download
  • 10 Web of Science
  • Crossref
Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam
Nguyen Thi Hong Chien, Thi Lan Nguyen, Khanh Linh Bui, Tho Van Nguyen, Thanh Hoa Le
Korean J Parasitol 2019;57(1):43-47.
Published online February 26, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.1.43
Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. A phylogenetic tree was inferred from 26 representative strains/species of Anaplasma spp. including 10 new sequences from Vietnam. Seven of our Vietnamese sequences fell into the clade of A. marginale and 3 into A. platys, with strong nodal support of 99 and 90%, respectively. Low genetic distances (0.2-0.4%) within each species supported the identification. Anaplasma platys is able to infect humans. Our discovery of this species in cattle and domestic dogs raises considerable concern about zoonotic transmission in Vietnam. Further systematic investigations are needed to gain data for Anaplasma spp. and members of Anaplasmataceae in animal hosts, vectors and humans across Vietnam.

Citations

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  • Molecular detection and genetic diversity of Anaplasma and Ehrlichia species in livestock and dogs in Pakistan
    Mughees Aizaz Alvi, Talha Javaid, Abu Bakar Yameen, Fakiha Asghar, Aliza Ali, Iqra Zaheer, Yousef Abdal Jalil Fadladdin, Hong-Bin Yan, Wan-Zhong Jia, Muhammad Saqib, Li Li
    Acta Tropica.2025; 268: 107700.     CrossRef
  • Isolation, Molecular Characterization and In Vitro Propagation of an Anaplasma platys-Like Bacterium in Tick Cells
    Erica Rodrigues de Matos, Priscilla Nunes dos Santos, Erich Peter Zweygarth, Talys Henrique Assumpção Jardim, Huarrisson Azevedo Santos, Matheus Dias Cordeiro, Bruna de Azevedo Baêta, Lesley Bell-Sakyi, Adivaldo Henrique da Fonseca, Claudia Bezerra da Sil
    Pathogens.2025; 14(9): 901.     CrossRef
  • Tick distribution and comparative analysis of bovine blood microbiome in two provinces of South Africa using 16S rRNA PacBio sequencing approach
    Bongekile L. Khoza, Charles Byaruhanga, S. Marcus Makgabo, Nkululeko Nyangiwe, Themba Mnisi, Samukelo Nxumalo, Marinda C. Oosthuizen, Zamantungwa T. H. Mnisi
    Frontiers in Tropical Diseases.2024;[Epub]     CrossRef
  • Molecular Characterization of Anaplasma spp. in Cattle from Kazakhstan
    Madina Kadyrova, Alexandr Ostrovskii, Kassym Mukanov, Amirkhan Kassen, Elena Shevtsova, Maxat Berdikulov, Gilles Vergnaud, Alexandr Shevtsov
    Pathogens.2024; 13(10): 894.     CrossRef
  • Detection of Tick‐Borne Pathogen Coinfections and Coexposures to Foot‐and‐Mouth Disease, Brucellosis, and Q Fever in Selected Wildlife From Kruger National Park, South Africa, and Etosha National Park, Namibia
    Carlo Andrea Cossu, Sunday Ochonu Ochai, Milana Troskie, Axel Hartmann, Jacques Godfroid, Lin-Mari de Klerk, Wendy Turner, Pauline Kamath, Ockert Louis van Schalkwyk, Rudi Cassini, Raksha Bhoora, Henriette van Heerden, Nan-Hua Chen
    Transboundary and Emerging Diseases.2024;[Epub]     CrossRef
  • Dynamics of Infections in Cattle and Rhipicephalus microplus: A Preliminary Study
    Elianne Piloto-Sardiñas, Angélique Foucault-Simonin, Alejandra Wu-Chuang, Lourdes Mateos-Hernández, Roxana Marrero-Perera, Lianet Abuin-Denis, Lisset Roblejo-Arias, Cristian Díaz-Corona, Zbigniew Zając, Joanna Kulisz, Aneta Woźniak, Sara Moutailler, Belki
    Pathogens.2023; 12(8): 998.     CrossRef
  • Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys in cattle in Nigeria
    Joshua Kamani, Juliane Schaer, Ali G. Umar, James Y. Pilarshimwi, Laminu Bukar, Javier González-Miguel, Shimon Harrus
    Ticks and Tick-borne Diseases.2022; 13(4): 101955.     CrossRef
  • Genetic diversity of Anaplasma marginale in calves with anaplasmosis on farms in Minas Gerais, Brazil
    Michele Bahia, Lucas Teixeira da Silva, Bruno Montijo da Silva, Matheus dias Cordeiro, Alexandro Guterres, Claudia Bezerra da Silva, Jenevaldo Barbosa da Silva, Adivaldo Henrique Fonseca
    Ticks and Tick-borne Diseases.2021; 12(1): 101552.     CrossRef
  • Comparison of Anaplasma and Ehrlichia species–specific peptide ELISAs with whole organism–based immunofluorescent assays for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs
    Barbara A. Qurollo, Brett A. Stillman, Melissa J. Beall, Paulette Foster, Barbara C. Hegarty, Edward B. Breitschwerdt, Ramaswamy Chandrashekar
    American Journal of Veterinary Research.2021; 82(1): 71.     CrossRef
  • Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia
    Viet-Linh Nguyen, Filipe Dantas-Torres, Domenico Otranto
    Current Research in Parasitology & Vector-Borne Diseases.2021; 1: 100001.     CrossRef
  • Molecular detection of selected tick-borne pathogens infecting cattle at the wildlife–livestock interface of Queen Elizabeth National Park in Kasese District, Uganda
    Benedicto Byamukama, Patrick Vudriko, Maria Agnes Tumwebaze, Dickson Stuart Tayebwa, Joseph Byaruhanga, Martin Kamilo Angwe, Jixu Li, Eloiza May Galon, Aaron Ringo, Mingming Liu, Yongchang Li, Shengwei Ji, Mohamed Abdo Rizk, Paul Frank Adjou Moumouni, Seu
    Ticks and Tick-borne Diseases.2021; 12(5): 101772.     CrossRef
  • Molecular detection of Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos, Trypanosoma evansi and first evidence of Theileria sinensis-associated bovine anaemia in crossbred Kedah-Kelantan x Brahman cattle
    Onyinyechukwu Ada Agina, Mohd Rosly Shaari, Nur Mahiza Md Isa, Mokrish Ajat, Mohd Zamri-Saad, Mazlina Mazlan, Azim Salahuddin Muhamad, Afrah Alhana Kassim, Lee Chai Ha, Fairuz Hazwani Rusli, Darulmuqaamah Masaud, Hazilawati Hamzah
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  • Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam
    Ly Na Huynh, Adama Zan Diarra, Quang Luan Pham, Nhiem Le-Viet, Jean-Michel Berenger, Van Hoang Ho, Xuan Quang Nguyen, Philippe Parola, Lynn Soong
    PLOS Neglected Tropical Diseases.2021; 15(9): e0009813.     CrossRef
  • The co-infection with Ehrlichia minasensis, Anaplasma marginale and Anaplasma platys is not associated with anemia in beef cattle in the Brazilian Pantanal
    Marcos Rogério André, Ana Cláudia Calchi, Heitor Miraglia Herrera, Diego Carlos de Souza Zanatto, Bruna de Carvalho Le Senéchal Horta, Júlia Banhareli Tasso, Inalda Angélica de Souza Ramos, Victória Valente Califre de Mello, Rosangela Zacarias Machado
    Veterinary Parasitology: Regional Studies and Reports.2020; 21: 100437.     CrossRef
  • Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand
    Anh H. L. Nguyen, Sonthaya Tiawsirisup, Morakot Kaewthamasorn
    BMC Veterinary Research.2020;[Epub]     CrossRef
  • 8,842 View
  • 132 Download
  • 17 Web of Science
  • Crossref

Original Article

Distribution Status of Hybrid Types in Large Liver Flukes, Fasciola Species (Digenea: Fasciolidae), from Ruminants and Humans in Vietnam
Thi Bich Nga Nguyen, Nguyen Van De, Thi Kim Lan Nguyen, Huynh Hong Quang, Huong Thi Thanh Doan, Takeshi Agatsuma, Thanh Hoa Le
Korean J Parasitol 2018;56(5):453-461.
Published online October 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.5.453
The aim of this study is to delineate ‘admixed hybrid’ and ‘introgressive’ Fasciola genotypes present in the Fasciola population in Vietnam. Adult liver flukes collected from ruminants in 18 Provinces were morphologically sorted out by naked eyes for small (S), medium (M) and large (L) body shapes; and human samples (n=14) from patients. Nuclear ribosomal (rDNA) ITS1 and ITS2, and mitochondrial (mtDNA) nad1 markers were used for determination of their genetic status. Total 4,725 worm samples of ruminants were tentatively classified by their size: 6% (n=284) small (S)-, 13% (n=614) medium (M)-, and 81% (n=3,827) large (L)-forms. All the representative (n=120, as 40 each group) and 14 human specimens, possessed maternal mtDNA of only F. gigantica and none of F. hepatica. Paternally, all (100%) of the L-(n=40) and 77.5% (n=31) of the M-flukes had single F. gigantica rDNA indicating ‘pure’ F. gigantica. A majority (90%, n=36) of the S- and 15% (n=6) of the M-worms had single F. hepatica rDNA, indicating their introgressive; the rest (10%, n=4) of the S- and 7.5% (n=3) of the M-flukes had mixture of both F. gigantica and F. hepatica rDNAs, confirming their admixed hybrid genetic status. Fourteen human samples revealed 9 (64%) of pure F. gigantica, 3 (22%) of introgressive and 2 (14%) of admixed hybrid Fasciola spp. By the present study, it was confirmed that the small worms, which are morphologically identical with F. hepatica, are admixed and/or introgressive hybrids of Fasciola spp., and able to be the pathogens of human fascioliasis.

Citations

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  • Integrating hybridization and introgression into host–parasite epidemiology, ecology, and evolution
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    Trends in Parasitology.2025; 41(2): 129.     CrossRef
  • Meta-analysis and systematic review of the prevalence and risk factors of animal fascioliasis in Eastern and Southern Africa between 2000 and 2023
    Stellah Nambuya, Chester Kalinda, Patrick Vudriko, Moses Adriko, Million Phiri, Tafadzwa Mindu, David Wagaba, Lawrence Mugisha
    Preventive Veterinary Medicine.2025; 239: 106490.     CrossRef
  • Challenges in the recognition of trematode species: Consideration of hypotheses in an inexact science
    T.H. Cribb, D.P. Barton, D. Blair, N.J. Bott, R.A. Bray, R.D. Corner, S.C. Cutmore, M.L.I. De Silva, B. Duong, A. Faltýnková, A. Gonchar, R.F. Hechinger, K.K. Herrmann, D.C. Huston, P.T.J. Johnson, G. Kremnev, R. Kuchta, C. Louvard, W.J. Luus-Powell, S.B.
    Journal of Helminthology.2025;[Epub]     CrossRef
  • Human fascioliasis emergence in southern Asia: Complete nuclear rDNA spacer and mtDNA gene sequences prove Indian patient infection related to fluke hybridization in northeastern India and Bangladesh
    M. Dolores Bargues, Patricio Artigas, George M. Varghese, T. Jacob John, Sitara S.R. Ajjampur, Syed Ali Ahasan, Emdadul Haque Chowdhury, Albis Francesco Gabrielli, Santiago Mas-Coma
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  • Environmental influence on abundance and infection patterns of snail intermediate hosts of liver and intestinal flukes in North and Central Vietnam
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    Parasites & Vectors.2024;[Epub]     CrossRef
  • A multidisciplinary analysis of over 53,000 fascioliasis patients along the 1995–2019 countrywide spread in Vietnam defines a new epidemiological baseline for One Health approaches
    Nguyen Van De, Pham Ngoc Minh, Thanh Hoa Le, Do Trung Dung, Tran Thanh Duong, Bui Van Tuan, Le Thanh Dong, Nguyen Van Vinh Chau, Pablo F. Cuervo, M. Dolores Bargues, M. Adela Valero, Albis Francesco Gabrielli, Antonio Montresor, Santiago Mas-Coma
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  • Fasciola hepatica and Fasciola hybrid form co-existence in yak from Tibet of China: application of rDNA internal transcribed spacer
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  • First morphometric and molecular characterization of Fasciola spp. in Northwest Tunisia
    Ines Hammami, Lavina Ciuca, Maria Paola Maurelli, Rihab Romdhane, Limam Sassi, Mohamed Ridha Rjeibi, Nadia Farhat, Alain Kouam Simo, Laura Rinaldi, Mourad Rekik, Mohamed Gharbi
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  • Description and phylogenetic analyses of ribosomal transcription units from species of Fasciolidae (Platyhelminthes: Digenea)
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  • Exposing the Barcoding Void: An Integrative Approach to Study Snail-Borne Parasites in a One Health Context
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  • Identification of Adult Fasciola spp. Using Matrix-Assisted Laser/Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry
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Case Report

A Rare Human Case of Dirofilaria repens Infection in the Subcutaneous Posterior Thorax with Molecular Identification
Tran Anh Le, Thuat Thang Vi, Khac Luc Nguyen, Thanh Hoa Le
Korean J Parasitol 2015;53(3):329-333.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.329
The emergence of dirofilarial infections in Asia including Vietnam is a clinically significant threat to the community. We here report a rare case of subcutaneous Dirofilaria repens infection on the posterior thoracic wall in a young woman presenting a painful, itchy, and palpable nodule. The adult worm was identified by mitochondrial cox1 and nuclear ITS-2 sequence determination. The diagnosis was additionally confirmed by 16S rRNA sequencing of the endosymbiont Wolbachia pipientis commonly co-existing with D. repens. This is a rare case of subcutaneous human infection on the posterior thoracic region caused by D. repens.

Citations

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    Esra Yilmaz, Moritz Fritzenwanker, Nikola Pantchev, Mathias Lendner, Sirichit Wongkamchai, Domenico Otranto, Inge Kroidl, Martin Dennebaum, Thanh Hoa Le, Tran Anh Le, Sabrina Ramünke, Roland Schaper, Georg von Samson-Himmelstjerna, Sven Poppert, Jürgen Kr
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  • 114 Download
  • 9 Web of Science
  • Crossref

Mini Review

Current Status of Taeniasis and Cysticercosis in Vietnam
Nguyen Van De, Thanh Hoa Le, Phan Thi Huong Lien, Keeseon S. Eom
Korean J Parasitol 2014;52(2):125-129.
Published online April 18, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.2.125

Several reports on taeniasis and cysticercosis in Vietnam show that they are distributed in over 50 of 63 provinces. In some endemic areas, the prevalence of taeniasis was 0.2-12.0% and that of cysticercosis was 1.0-7.2%. The major symptoms of taeniasis included fidgeted anus, proglottids moving out of the anus, and proglottids in the feces. Clinical manifestations of cysticercosis in humans included subcutaneous nodules, epileptic seizures, severe headach, impaired vision, and memory loss. The species identification of Taenia in Vietnam included Taenia asiatica, Taenia saginata, and Taenia solium based on combined morphology and molecular methods. Only T. solium caused cysticercosis in humans. Praziquantel was chosen for treatment of taeniasis and albendazole for treatment of cysticercosis. The infection rate of cysticercus cellulosae in pigs was 0.04% at Hanoi slaughterhouses, 0.03-0.31% at provincial slaughterhouses in the north, and 0.9% in provincial slaughterhouses in the southern region of Vietnam. The infection rate of cysticercus bovis in cattle was 0.03-2.17% at Hanoi slaughterhouses. Risk factors investigated with regard to transmission of Taenia suggested that consumption of raw meat (eating raw meat 4.5-74.3%), inadequate or absent meat inspection and control, poor sanitation in some endemic areas, and use of untreated human waste as a fertilizer for crops may play important roles in Vietnam, although this remains to be validated.

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Case Reports

The First Human Case of Thelazia callipaeda Infection in Vietnam
Nguyen Van De, Thanh Hoa Le, Jong-Yil Chai
Korean J Parasitol 2012;50(3):221-223.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.221

A 26-year-old man residing in a village of Thai Nguyen Province, North Vietnam, visited the Thai Nguyen Provincial Hospital in July 2008. He felt a bulge-sticking pain in his left eye and extracted 5 small nematode worms by himself half a day before visiting the hospital. Two more worms were extracted from his left eye by a medical doctor, and they were morphologically observed and genetically analyzed on the mitochondrial cytochrome c oxidase 1 gene. The worms were 1 male and 1 female, and genetically identical with those of Thelazia callipaeda. By the present study, the presence of human T. callipaeda infection is first reported in Vietnam.

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From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.

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Original Article
Codon usage and bias in mitochondrial genomes of parasitic platyhelminthes
Thanh Hoa Le, Donald Peter McManus, David Blair
Korean J Parasitol 2004;42(4):159-167.
Published online December 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.4.159

Sequences of the complete protein-coding portions of the mitochondrial (mt) genome were analysed for 6 species of cestodes (including hydatid tapeworms and the pork tapeworm) and 5 species of trematodes (blood flukes and liver- and lung-flukes). A near-complete sequence was also available for an additional trematode (the blood flukeSchistosoma malayensis). All of these parasites belong to a large flatworm taxon named the Neodermata. Considerable variation was found in the base composition of the protein-coding genes among these neodermatans. This variation was reflected in statistically-significant differences in numbers of each inferred amino acid between many pairs of species. Both convergence and divergence in nucleotide, and hence amino acid, composition was noted among groups within the Neodermata. Considerable variation in skew (unequal representation of complementary bases on the same strand) was found among the species studied. A pattern is thus emerging of diversity in the mt genome in neodermatans that may cast light on evolution of mt genomes generally.

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