Parasites, Hosts and Diseases

"Wanchai Maleewong"

Brief Communications

Evaluation of IgG4 Subclass Antibody Detection by Peptide-Based ELISA for the Diagnosis of Human Paragonimiasis Heterotrema: Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Porntip Laummaunwai, Nimit Morakote, Yoon Kong, Wanchai Maleewong; Korean J Parasitol 2013;51(6):763-766.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.763

Abstract
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A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values of 100%, 94.6%, 96.2%, 100%, and 88.9%, respectively. Cross reactivity was frequently seen against the sera of fascioliasis (75%) and hookworm infections (50%). Since differential diagnosis between paragonimiasis and fascioliasis can be easily done by clinical presentation and fascioliasis serology, this cross reaction is not a serious problem. Sera from patients with other parasitoses (0-25%) rarely responded to this synthetic antigen. This synthetic peptide antigen seems to be useful for development of a standardized diagnostic system for paragonimiasis.

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An immunochromatographic test using whole blood for rapid diagnosis of human paragonimiasis and its diagnostic usefulness
Patcharaporn Boonroumkaew, Lakkhana Sadaow, Penchom Janwan, Rutchanee Rodpai, Oranuch Sanpool, Tongjit Thanchomnang, Hiroshi Yamasaki, Pewpan M. Intapan, Wanchai Maleewong
Food and Waterborne Parasitology.2024; 37: e00246. CrossRef
Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis
Lakkhana Sadaow, Oranuch Sanpool, Hiroshi Yamasaki, Wanchai Maleewong, Pewpan M. Intapan
Acta Tropica.2020; 203: 105325. CrossRef
Paragonimus and paragonimiasis in Asia: An update
Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama
Acta Tropica.2019; 199: 105074. CrossRef
A Case of Pulmonary Paragonimiasis with Chronic Abdominal Pain and Erythematous Rash in a 6-year-old Girl
Ju Young Kim, Min Kyu Park, Yong Ju Lee, Sun Huh, Ky Young Cho
Pediatric Infection and Vaccine.2018; 25(1): 54. CrossRef
A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
Xu Guang Qiu, Fukumi Nakamura-Uchiyama, Yukifumi Nawa, Makoto Itoh
Tropical Medicine and Health.2016;[Epub] CrossRef
Expression characteristics and specific antibody reactivity of diverse cathepsin F members of Paragonimus westermani
Chun-Seob Ahn, Byoung-Kuk Na, Dong-ll Chung, Jeong-Geun Kim, Jin-Taek Kim, Yoon Kong
Parasitology International.2015; 64(1): 37. CrossRef
North American paragonimiasis: epidemiology and diagnostic strategies
Peter U Fischer, Gary J Weil
Expert Review of Anti-infective Therapy.2015; 13(6): 779. CrossRef

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Detection of Gnathostoma spinigerum Antibodies in Sera of Non-Traumatic Subarachnoid Hemorrhage Patients in Thailand: Amnat Kitkhuandee, Waranon Munkong, Kittisak Sawanyawisuth, Penchom Janwan, Wanchai Maleewong, Pewpan M. Intapan; Korean J Parasitol 2013;51(6):755-757.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.755

Abstract
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Gnathostoma spinigerum can cause subarachnoid hemorrhage (SAH). The detection of specific antibodies in serum against G. spinigerum antigen is helpful for diagnosis of neurognathostomiasis. There is limited data on the frequency of G. spinigerum infection in non-traumatic SAH. A series of patients diagnosed as non-traumatic SAH at the Srinagarind Hospital, Khon Kaen University, Thailand between January 2011 and January 2013 were studied. CT or MR imaging of the brain was used for diagnosis of SAH. Patients were categorized as aneurysmal subarachnoid hemorrhage (A-SAH) or non-aneurysmal subarachnoid hemorrhage (NA-SAH) according to the results of cerebral angiograms. The presence of specific antibodies in serum against 21- or 24-kDa G. spinigerum antigen was determined using the immunoblot technique. The detection rate of antibodies was compared between the 2 groups. Of the 118 non-traumatic SAH patients for whom cerebral angiogram and immunoblot data were available, 80 (67.8%) patients had A-SAH, whereas 38 (32.2%) had NA-SAH. Overall, 23.7% were positive for specific antibodies against 21- and/or 24-kDa G. spinigerum antigen. No significant differences were found in the positive rate of specific antibodies against G. spinigerum in both groups (P-value=0.350).

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A Recombinant Matrix Metalloproteinase Protein from Gnathostoma spinigerum for Serodiagnosis of Neurognathostomiasis: Penchom Janwan, Pewpan M. Intapan, Hiroshi Yamasaki, Porntip Laummaunwai, Kittisak Sawanyawisuth, Chaisiri Wongkham, Chatchai Tayapiwatana, Amnat Kitkhuandee, Viraphong Lulitanond, Yukifumi Nawa, Wanchai Maleewong; Korean J Parasitol 2013;51(6):751-754.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.751

Abstract
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Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components of molecular mass 21 kDa and 24 kDa in larval extracts of Gnathostoma spinigerum (Gs 21/24), has high sensitivity and specificity for diagnosis of neurognathostomiasis. However, only very small amounts of the Gs 21/24 antigens can be prepared from parasites harvested from natural or experimental animals. To overcome this problem, we recently produced a recombinant matrix metalloproteinase (rMMP) protein from G. spinigerum. In this study, we evaluated this rMMP alongside the Gs 21/24 antigens for serodiagnosis of human neurognathostomiasis. We studied sera from 40 patients from Srinagarind Hospital, Khon Kaen University, Thailand, with clinical criteria consistent with those of neurognathostomiasis, and sera from 30 healthy control adults from Thailand. All sera were tested for specific IgG antibodies against both G. spinigerum crude larval extract and rMMP protein using immunoblot analysis. The sensitivity and specificity for both antigenic preparations were all 100%. These results show that G. spinigerum rMMP protein can be used as an alternative diagnostic antigen, in place of larval extract, for serodiagnosis of neurognathostomiasis.

Citations

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Protein and antigen profiles of third-stage larvae of Gnathostoma spinigerum assessed with next-generation sequencing transcriptomic information
Kathyleen Nogrado, Tipparat Thiangtrongjit, Poom Adisakwattana, Paron Dekumyoy, Sant Muangnoicharoen, Charin Thawornkuno, Onrapak Reamtong
Scientific Reports.2022;[Epub] CrossRef
Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis
Penchom Janwan, Pewpan M. Intapan, Lakkhana Sadaow, Rutchanee Rodpai, Hiroshi Yamasaki, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Phuangphaka Sadee, Wanchai Maleewong
Diagnostics.2021; 11(5): 862. CrossRef
Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development
Tipparat Thiangtrongjit, Kathyleen Nogrado, Thawatchai Ketboonlue, Preeyarat Malaitong, Poom Adisakwattana, Onrapak Reamtong
Pathogens.2021; 10(9): 1080. CrossRef
Combining lexical and context features for automatic ontology extension
Sara Althubaiti, Şenay Kafkas, Marwa Abdelhakim, Robert Hoehndorf
Journal of Biomedical Semantics.2020;[Epub] CrossRef
Human gnathostomiasis: a neglected food-borne zoonosis
Guo-Hua Liu, Miao-Miao Sun, Hany M. Elsheikha, Yi-Tian Fu, Hiromu Sugiyama, Katsuhiko Ando, Woon-Mok Sohn, Xing-Quan Zhu, Chaoqun Yao
Parasites & Vectors.2020;[Epub] CrossRef
Surveillance and diagnosis of zoonotic foodborne parasites
Reza Zolfaghari Emameh, Sami Purmonen, Antti Sukura, Seppo Parkkila
Food Science & Nutrition.2018; 6(1): 3. CrossRef

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Molecular Detection of Ancylostoma duodenale, Ancylostoma ceylanicum, and Necator americanus in Humans in Northeastern and Southern Thailand: Issarapong Phosuk, Pewpan M. Intapan, Tongjit Thanchomnang, Oranuch Sanpool, Penchom Janwan, Porntip Laummaunwai, Witthaya Aamnart, Nimit Morakote, Wanchai Maleewong; Korean J Parasitol 2013;51(6):747-749.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.747

Abstract
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The 2 principal species of hookworms infecting humans are Necator americanus and Ancylostoma duodenale. Case studies on zoonotic hookworm infections with Ancylostoma ceylanicum and/or Ancylostoma caninum are known mainly from Asian countries. Of these 2 zoonotic species, only A. ceylanicum can develop to adulthood in humans. In the present study, we report a molecular-based survey of human hookworm infections present in southern and northeastern Thailand. Thirty larval hookworm samples were obtained from fecal agar plate cultures of 10 patients in northeastren Thailand and 20 in southern Thailand. Partial ITS1, 5.8S, and ITS2 regions of the ribosomal DNA genes were amplified using PCR. The amplicons were sequenced, aligned, and compared with other hookworm sequences in GenBank database. The results showed that, in Thailand, N. americanus is more prevalent than Ancylostoma spp. and is found in both study areas. Sporadic cases of A. ceylanicum and A. duodenale infection were seen in northeastern Thailand.

Citations

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Efficacy of Simparica Trio® against induced infections of Ancylostoma braziliense and Ancylostoma ceylanicum in dogs
Raj Packianathan, Andrew Hodge, Natalie Bruellke, Michael Pearce, Frans Selepe, Piyanan Taweethavonsawat, Thomas Geurden
Parasites & Vectors.2025;[Epub] CrossRef
The occurrence of cross-host species soil-transmitted helminth infections in humans and domestic/livestock animals: A systematic review
Uniqueky Gratis Mawrie, Riviarynthia Kharkongor, María Martínez Valladares, Stella Kepha, Sitara S. R. Ajjampur, Rajiv Sarkar, Rachel Pullan, Muhammad Asaduzzaman
PLOS Global Public Health.2025; 5(8): e0004614. CrossRef
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Mamta Thakur, Abhishek Mewara, P. V. M. Lakshmi, Sucheta Guleria, Sumeeta Khurana
Scientific Reports.2025;[Epub] CrossRef
PERFIL PARASITOLÓGICO DE HORTALIÇAS CULTIVADAS EM HORTAS TRADICIONAIS
Maria Beatriz Neves Nascimento, Monalissa Dias de Souza, Alexandro de Andrade de Lima, Gislaine Cristina de Souza Melanda, Renato Juciano Ferreira
Revista Saúde - UNG-Ser.2025;[Epub] CrossRef
Ancylostoma ceylanicum and other zoonotic canine hookworms: neglected public and animal health risks in the Asia–Pacific region
Jan Clyden B. Tenorio, Ian Kim B. Tabios, Tawin Inpankaew, Adrian P. Ybañez, Saruda Tiwananthagorn, Sirikachorn Tangkawattana, Sutas Suttiprapa
Animal Diseases.2024;[Epub] CrossRef
Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand
Rutchanee Rodpai, Oranuch Sanpool, Lakkhana Sadaow, Patcharaporn Boonroumkaew, Pewpan M. Intapan, Wanchai Maleewong, Manachai Yingklang, Penchom Janwan, Kotchaphon Vaisusuk, Wasupon Chatan, Supawadee Piratae, Tongjit Thanchomnang
Parasitology Research.2024;[Epub] CrossRef
The enhancive effect of the 2014–2016 El Niño-induced drought on the control of soil-transmitted helminthiases without anthelmintics: A longitudinal study
Attarat Pattanawongsa, Pattanasak Kammaneechan, Prasit Na-ek, Blego Sedionoto, Witthaya Anamnart, Qu Cheng
PLOS Neglected Tropical Diseases.2024; 18(7): e0012331. CrossRef
Assessment of an Immuno-Diagnostic Method for Hookworm-Related Cutaneous Larva Migrans Using Crude Extracts of Ancylostoma caninum
Sitthithana Adam, Paron Dekumyoy, Duangporn Nacapunchai, Thawatchai Ketboonlue, Prakaykaew Charunwatthana, Jittima Dhitavat, Khuanchai Koompapong, Putza Chonsawat, Dorn Watthanakulpanich
Tropical Medicine and Infectious Disease.2023; 8(4): 209. CrossRef
Global distribution of human hookworm species and differences in their morbidity effects: a systematic review
Archie C A Clements, Kefyalew Addis Alene
The Lancet Microbe.2022; 3(1): e72. CrossRef
Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response
Richard D. Bungiro, Lisa M. Harrison, Blaise Dondji, Michael Cappello, Maria Angeles Gómez-Morales
PLOS Neglected Tropical Diseases.2022; 16(1): e0010098. CrossRef
Prevalence of intestinal parasitic infections and genetic differentiation of Strongyloides stercoralis among migrant workers from Myanmar, Lao PDR and Cambodia in northeastern Thailand
Wararat Sangwalee, Jun Norkaew, Sengchoy Inthachak, Penchom Janwan, Rutchanee Rodpai, Oranuch Sanpool, Lakkhana Sadaow, Patcharaporn Boonroumkaew, Pewpan M. Intapan, Wanchai Maleewong, Tongjit Thanchomnang, Raffi V. Aroian
PLOS ONE.2022; 17(12): e0279754. CrossRef
Copro-molecular identification of intestinal nematode infections in a rural community in East Malaysia
Nur-Amirah Hassan, Autchariya Thodsapol, Yvonne Ai Lian Lim, Wan Yusoff Wan Sulaiman, Siti Farah Norasyikeen Sidi Omar, Kamontam Umsakul, Romano Ngui
Parasitology International.2021; 80: 102237. CrossRef
Soil-transmitted helminths, intestinal protozoa and Clonorchis sinensis infections in southeast China
Yan Feng, Kegen Yu, Hualiang Chen, Xuan Zhang, Qiaoyi Lu, Xiaoxiao Wang, Xueying Zhang, Linong Yao, Wei Ruan
BMC Infectious Diseases.2021;[Epub] CrossRef
Zoonotic hookworms of dogs and cats – lessons from the past to inform current knowledge and future directions of research
Rebecca J. Traub, Patsy A. Zendejas-Heredia, Luca Massetti, Vito Colella
International Journal for Parasitology.2021; 51(13-14): 1233. CrossRef
Molecular identification of hookworm isolates from stray dogs, humans and selected wildlife from South Africa
P. I. Ngcamphalala, J. Lamb, S. Mukaratirwa
Journal of Helminthology.2020;[Epub] CrossRef
Semi-domesticated dogs as a potential reservoir for zoonotic hookworms in Bangkok, Thailand
Jutamas Wongwigkan, Tawin Inpankaew
Veterinary World.2020; 13(5): 909. CrossRef
The increased sensitivity of qPCR in comparison to Kato-Katz is required for the accurate assessment of the prevalence of soil-transmitted helminth infection in settings that have received multiple rounds of mass drug administration
Julia C. Dunn, Marina Papaiakovou, Kay Thwe Han, Darren Chooneea, Alison A. Bettis, Nay Yee Wyine, Aye Moe Moe Lwin, Nay Soe Maung, Raju Misra, D. T. J. Littlewood, Roy M. Anderson
Parasites & Vectors.2020;[Epub] CrossRef
Zoonotic Ancylostomiasis: An Update of a Continually Neglected Zoonosis
Katharina Stracke, Aaron R. Jex, Rebecca J. Traub
The American Journal of Tropical Medicine and Hygiene.2020; 103(1): 64. CrossRef
Of dogs and hookworms: man’s best friend and his parasites as a model for translational biomedical research
Catherine Shepherd, Phurpa Wangchuk, Alex Loukas
Parasites & Vectors.2018;[Epub] CrossRef
Prevalence of Hookworm infection and Strongyloidiasis in Cats and Potential Risk Factor of Human Diseases
Blego Sedionoto, Witthaya Anamnart, Hadiyanto, Sudarno, Maryono
E3S Web of Conferences.2018; 31: 06002. CrossRef
Molecular characterization of hookworm spp. isolated from food handlers, Khartoum, Sudan: A cross-sectional study
Tarig A. Gamar, Hassan H. Musa, Hisham N. Altayb, Mohamed H. Mohamed, Adam D. Abakar
F1000Research.2018; 7: 662. CrossRef
Ancylostoma ceylanicum Hookworm in Myanmar Refugees, Thailand, 2012–2015
Elise M. O’Connell, Tarissa Mitchell, Marina Papaiakovou, Nils Pilotte, Deborah Lee, Michelle Weinberg, Potsawin Sakulrak, Dilok Tongsukh, Georgiette Oduro-Boateng, Sarah Harrison, Steven A. Williams, William M. Stauffer, Thomas B. Nutman
Emerging Infectious Diseases.2018;[Epub] CrossRef
Development and Evaluation of a Multiplex Quantitative Real-Time Polymerase Chain Reaction for Hookworm Species in Human Stool
Sze Fui Hii, Dammika Senevirathna, Stacey Llewellyn, Tawin Inpankaew, Peter Odermatt, Virak Khieu, Sinoun Muth, James McCarthy, Rebecca J. Traub
The American Journal of Tropical Medicine and Hygiene.2018; 99(5): 1186. CrossRef
Hookworm infections among migrant workers in Malaysia: Molecular identification of Necator americanus and Ancylostoma duodenale
Norhidayu Sahimin, Yvonne Ai Lian Lim, Benacer Douadi, Mohd Khairul Nizam Mohd Khalid, John-James Wilson, Jerzy M. Behnke, Siti Nursheena Mohd Zain
Acta Tropica.2017; 173: 109. CrossRef
Prevalence of intestinal parasitic infection and associated risk factors among village health volunteers in rural communities of southern Thailand
Chuchard Punsawad, Nonthapan Phasuk, Suchirat Bunratsami, Kanjana Thongtup, Niramon Siripakonuaong, Somchok Nongnaul
BMC Public Health.2017;[Epub] CrossRef
Soil-Transmitted Helminths in Tropical Australia and Asia
Catherine Gordon, Johanna Kurscheid, Malcolm Jones, Darren Gray, Donald McManus
Tropical Medicine and Infectious Disease.2017; 2(4): 56. CrossRef
Restoration of hookworm egg development after prolonged storage in stool suspension
Prasit Na-Ek, Oranuch Sanpool, Jurairat Jongthawin, Witthaya Anamnart, Pewpan M. Intapan, Pennapa Chamavit, Wanchai Maleewong
Parasitology Research.2016; 115(7): 2817. CrossRef
Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand
Wilawan PUMIDONMING, Doaa SALMAN, Dulyatad GRONSANG, Abdelbaset E. ABDELBASET, Khamphon SANGKAEO, Shin-ichiro KAWAZU, Makoto IGARASHI
Journal of Veterinary Medical Science.2016; 78(12): 1779. CrossRef

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Modified Formalin-Ether Concentration Technique for Diagnosis of Human Strongyloidiasis: Witthaya Anamnart, Pewpan M. Intapan, Wanchai Maleewong; Korean J Parasitol 2013;51(6):743-745.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.743

Abstract
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We compared the efficacy and applicability of a modified formalin-ether concentration technique (M-FECT) to the conventional FECT (C-FECT) and the agar plate culture (APC) method for the detection of Strongyloides stercoralis larvae. For this purpose, we used 600 human fecal specimens collected in an endemic area of southern Thailand. In the M-FECT, we used 2 layers of wire meshes, instead of gauze, to avoid the loss by absorption/adhesion of larvae to the gauze during filtration, and we reduced the exposure time of S. stercoralis larvae in stool samples to formalin. By such simple modifications, the efficacy of M-FECT has become comparable to APC and was much better than that of C-FECT for the diagnosis of strongyloidiasis.

Citations

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Gastrointestinal Parasitic Infections in Macaca fascicularis in Northeast Thailand: A One Health Perspective on Zoonotic Risks
Teputid Kuasit, Manachai Yingklang, Penchom Janwan, Wanchai Maleewong, Weerachai Saijuntha, Siriporn Kuanamon, Tongjit Thanchomnang
Animals.2025; 15(14): 2112. CrossRef
Strongyloidiasis
Catherine A. Gordon, Jürg Utzinger, Stephen Muhi, Sören L. Becker, Jennifer Keiser, Virak Khieu, Darren J. Gray
Nature Reviews Disease Primers.2024;[Epub] CrossRef
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Vania Agustina, Prasert Saichua, Thewarach Laha, Sirikachorn Tangkawatana, Suksanti Prakobwong, Nonglak Laoprom, Wanrak Kamphasri, Chonteera Chareonchai, David Blair, Sutas Suttiprapa
International Journal for Parasitology.2024; 54(10): 497. CrossRef
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Apisit Chaidee, Naruechar Charoenram, Chatchawan Sengthong, Rungtiwa Dangtakot, Porntip Pinlaor, Thatsanapong Pongking, Somchai Pinlaor
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Sengaloun Phonekeo, Sengchanh Kounnavong, Manithong Vonglokham, Latsamy Siengsounthone, Anousin Homsana, Sascha Gummin, Penelope Vounatsu, Prawat Nittiyanant, Suchin Worawichawong, Wichai Aekplakorn, Peter Odermatt, Somphou Sayasone
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Watcharapol Suyapoh, Domechai Kaewnoi, Pornphutthachat Sota, Wichaya Thongtako, Sutas Suttiprapa
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Pornphutthachat Sota, Sutas Suttiprapa, Sirikachorn Tangkawattana, Manop Sripa, David Blair, Banchob Sripa
Parasitology.2022; 149(10): 1334. CrossRef
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Ivana Camacho-Alvarez, Elia M. Chavez-Mamani, Goyens Philippe, Jenny M. Luizaga-López, Mary Cruz Torrico, Laurent Gétaz, Frédérique Jacobs
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Wannee Kaewrat, Chatchawan Sengthong, Manachai Yingklang, Kitti Intuyod, Ornuma Haonon, Sudarat Onsurathum, Rungtiwa Dangtakot, Phitsamai Saisud, Arunnee Sangka, Sirirat Anutrakulchai, Somchai Pinlaor, Ubon Cha'on, Porntip Pinlaor
Acta Tropica.2020; 203: 105291. CrossRef
Comparative study of two commercial tests for Strongyloides stercoralis serologic diagnosis
Isabel Fradejas, J M Herrero-Martínez, Manuel Lizasoaín, Esperanza Rodríguez de las Parras, Ana Pérez-Ayala
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Asian Biomedicine.2018; 12(1): 45. CrossRef
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Jennifer K. Ketzis
Acta Tropica.2017; 170: 178. CrossRef
Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand
Wilawan PUMIDONMING, Doaa SALMAN, Dulyatad GRONSANG, Abdelbaset E. ABDELBASET, Khamphon SANGKAEO, Shin-ichiro KAWAZU, Makoto IGARASHI
Journal of Veterinary Medical Science.2016; 78(12): 1779. CrossRef
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Case Reports

Sparganosis Presenting as Cauda Equina Syndrome with Molecular Identification of the Parasite in Tissue Sections: Adhiratha Boonyasiri, Pornsuk Cheunsuchon, Prajak Srirabheebhat, Hiroshi Yamasaki, Wanchai Maleewong, Pewpan M. Intapan; Korean J Parasitol 2013;51(6):739-742.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.739

Abstract
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A 52-year-old woman presented with lower back pain, progressive symmetrical paraparesis with sensory impairment, and sphincter disturbance. Magnetic resonance imaging (MRI) of the whole spine revealed multiple intradural extramedullary serpiginous-mass lesions in the subarachnoid space continuously from the prepontine to the anterior part of the medulla oblongata levels, C7, T2-T8, and T12 vertebral levels distally until the end of the theca sac and filling-in the right S1 neural foramen. Sparganosis was diagnosed by demonstration of the sparganum in histopathological sections of surgically resected tissues and also by the presence of serum IgG antibodies by ELISA. DNA was extracted from unstained tissue sections, and a partial fragment of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was amplified using a primer set specific for Spirometra spp. cox1. After sequencing of the PCR-amplicon and alignment of the nucleotide sequence data, the causative agent was identified as the larva of Spirometra erinaceieuropaei.

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Ryo Miyahara, Osamu Akiyama, Naoko Yoshida, Mai Suzuki, Karin Ashizawa, Takuma Kodama, Yuzaburo Shimizu, Akihide Kondo
Surgical Neurology International.2025; 16: 512. CrossRef
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Parasitology International.2024; 98: 102798. CrossRef
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Formosan Journal of Surgery.2023; 56(5): 160. CrossRef
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Gan-Jun Wen, Jian Chen, Shi-Fei Zhang, Zhi-Sen Zhou, Gen-Long Jiao
World Journal of Clinical Cases.2023; 11(36): 8507. CrossRef
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Clinical Manifestations of Eosinophilic Meningitis Due to Infection with Angiostrongylus cantonensis in Children: Kittisak Sawanyawisuth, Jarin Chindaprasirt, Vichai Senthong, Panita Limpawattana, Narong Auvichayapat, Sompon Tassniyom, Verajit Chotmongkol, Wanchai Maleewong, Pewpan M. Intapan; Korean J Parasitol 2013;51(6):735-738.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.735

Abstract
PDF

Eosinophilic meningitis, caused by the nematode Angiostrongylus cantonensis, is prevalent in northeastern Thailand, most commonly in adults. Data regarding clinical manifestations of this condition in children is limited and may be different those in adults. A chart review was done on 19 eosinophilic meningitis patients aged less than 15 years in Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Thailand. Clinical manifestations and outcomes were reported using descriptive statistics. All patients had presented with severe headache. Most patients were males, had fever, nausea or vomiting, stiffness of the neck, and a history of snail ingestion. Six patients had papilledema or cranial nerve palsies. It was shown that the clinical manifestations of eosinophilic meningitis due to A. cantonensis in children are different from those in adult patients. Fever, nausea, vomiting, hepatomegaly, neck stiffness, and cranial nerve palsies were all more common in children than in adults.

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Original Articles

Molecular Differentiation of Opisthorchis viverrini and Clonorchis sinensis Eggs by Multiplex Real-Time PCR with High Resolution Melting Analysis: Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Porntip Laummaunwai, Viraphong Lulitanond, Pham Ngoc Doanh, Wanchai Maleewong; Korean J Parasitol 2013;51(6):689-694.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.689

Abstract
PDF

Opisthorchis viverrini and Clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in Asia. The standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. However, the method has low sensitivity, and eggs of O. viverrini and C. sinensis are difficult to distinguish from each other and from those of some other trematodes. Here, we report a multiplex real-time PCR coupled with high resolution melting (HRM) analysis for the differentiation of O. viverrini and C. sinensis eggs in fecal samples. Using 2 pairs of species-specific primers, DNA sequences from a portion of the mitochondrial NADH dehydrogenase subunit 2 (nad 2) gene, were amplified to generate 209 and 165 bp products for O. viverrini and C. sinensis, respectively. The distinct characteristics of HRM patterns were analyzed, and the melting temperatures peaked at 82.4±0.09℃ and 85.9±0.08℃ for O. viverrini and C. sinensis, respectively. This technique was able to detect as few as 1 egg of O. viverrini and 2 eggs of C. sinensis in a 150 mg fecal sample, which is equivalent to 7 and 14 eggs per gram of feces, respectively. The method is species-specific, rapid, simple, and does not require fluorescent probes or post-PCR processing for discrimination of eggs of the 2 species. It offers a new tool for differentiation and detection of Asian liver fluke infections in stool specimens.

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Fish and Food-Fatale: Food-borne Trematode Opisthorchis viverrini and Cholangiocarcinoma
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Molecular Variation in the Paragonimus heterotremus Complex in Thailand and Myanmar: Oranuch Sanpool, Pewpan M. Intapan, Tongjit Thanchomnang, Penchom Janwan, Yukifumi Nawa, David Blair, Wanchai Maleewong; Korean J Parasitol 2013;51(6):677-681.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.677

Abstract
PDF

Paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. Of the 7 members of the genus known in Thailand until recently, only P. heterotremus has been confirmed as causing human disease. An 8th species, P. pseudoheterotremus, has recently been proposed from Thailand, and has been found in humans. Molecular data place this species as a sister species to P. heterotremus, and it is likely that P. pseudoheterotremus is not specifically distinct from P. heterotremus. In this study, we collected metacercariae of both nominal species (identification based on metacercarial morphology) from freshwater crabs from Phetchabun Province in northern Thailand, Saraburi Province in central Thailand, and Surat Thani Province in southern Thailand. In addition, we purchased freshwater crabs imported from Myanmar at Myawaddy Province, western Thailand, close to the Myanmar-Thailand border. The DNAs extracted from excysted metacercariae were PCR-amplified and sequenced for ITS2 and cox1 genes. The ITS2 sequences were nearly identical among all samples (99-100%). Phylogenies inferred from all available partial cox1 sequences contained several clusters. Sequences from Indian P. heterotremus formed a sister group to sequences from P. pseudoheterotremus-type metacercariae. Sequences of P. heterotremus from Thailand, Vietnam, and China formed a separate distinct clade. One metacercaria from Phitsanulok Province was distinct from all others. There is clearly considerable genetic variation in the P. heterotremus complex in Thailand and the form referred to as P. pseudoheterotremus is widely distributed in Thailand and the Thai-Myanmar border region.

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Considerable genetic diversity within Paragonimus heterotremus in Luang Prabang, northern Lao People's Democratic Republic
Alongkorn Nonthapa, Rutchanee Rodpai, Tongjit Thanchomnang, Patcharaporn Boonroumkaew, Lakkhana Sadaow, David Blair, Pewpan M. Intapan, Wanchai Maleewong, Virasack Banouvong, Sakhone Laymanivong, Oranuch Sanpool
Infection, Genetics and Evolution.2025; 128: 105718. CrossRef
Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): genetic characteristics, polymorphism, and implications for intersuperfamilial phylogeny
Khue Thi Nguyen, Huong Thi Thanh Doan, Linh Thi Khanh Pham, Do Thi Roan, Takeshi Agatsuma, Pham Ngoc Doanh, Thanh Hoa Le
Parasitology Research.2024;[Epub] CrossRef
Morphological and Molecular Characterization of Paragonimus skrjabini Complex from Yunnan, China: A Brief Report
Qiu-Hong Shu, Shu-De Li, Ming Tian, Yong Meng, Shu-Mei-Qi He, Min Zhu, Miao-Miao Wang, Wen-Lin Wang
Acta Parasitologica.2022; 67(1): 316. CrossRef
The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes
Abigail Hui En Chan, Naowarat Saralamba, Sompob Saralamba, Jiraporn Ruangsittichai, Urusa Thaenkham
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Jong-Yil Chai, Bong-Kwang Jung
Parasitology.2022; 149(10): 1262. CrossRef
Genetic differentiation of Southeast Asian Paragonimus Braun, 1899 (Digenea: Paragonimidae) and genetic variation in the Paragonimus heterotremus complex examined by nuclear DNA sequences
Chairat Tantrawatpan, Sumonta Tapdara, Takeshi Agatsuma, Oranuch Sanpool, Pewpan M. Intapan, Wanchai Maleewong, Weerachai Saijuntha
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An outbreak of Paragonimus westermani infection among Cambodian technical intern trainees in Japan, exhibiting various extrapulmonary lesions
Yoshifumi Nakagawa, Yuki Ikematsu, Takayuki Nakanishi, Yuji Ogawa, Ryuichi Taen, Yasunori Nakashima, Hironao Okabe, Ayako Yoshida, Haruhiko Maruyama
Parasitology International.2021; 81: 102279. CrossRef
Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis
Lakkhana Sadaow, Oranuch Sanpool, Hiroshi Yamasaki, Wanchai Maleewong, Pewpan M. Intapan
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Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama
Acta Tropica.2019; 199: 105074. CrossRef
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Carolina Lenis, Alicia Galiano, Imelda Vélez, Iván Darío Vélez, Carlos Muskus, Antonio Marcilla
Acta Tropica.2018; 183: 95. CrossRef
Gene diversity and genetic variation in lung flukes (genusParagonimus)
David Blair, Yukifumi Nawa, Makedonka Mitreva, Pham Ngoc Doanh
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Helminthologia.2015; 52(3): 167. CrossRef

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Ultrastructure of Spermatogenesis in the Testis of Paragonimus heterotremus: Nongnut Uabundit, Pipatphong Kanla, Phongphithak Puthiwat, Channarong Arunyanart, Kowit Chaiciwamongkol, Wanchai Maleewong, Pewpan M. Intapan, Sitthichai Iamsaard, Wiphawi Hipkaeo; Korean J Parasitol 2013;51(6):669-676.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.669

Abstract
PDF

Lung fluke, Paragonimus heterotremus, is a flatworm causing pulmonary paragonimiasis in cats, dogs, and humans in Southeast Asia. We examined the ultrastructure of the testis of adult P. heterotremus with special attention to spermatogenesis and spermiogenesis using scanning and transmission electron microscopy. The full sequence of spermatogenesis and spermiogenesis, from the capsular basal lamina to the luminal surface, was demonstrated. The sequence comprises spermatogonia, spermatocytes with obvious nuclear synaptonemal complexes, spermatids, and eventual spermatozoa. Moreover, full steps of spermatid differentiation were shown which consisted of 1) early stage, 2) differentiation stage representing the flagella, intercentriolar body, basal body, striated rootlets, and electron dense nucleus of thread-like lamellar configuration, and 3) growing spermatid flagella. Detailed ultrastructure of 2 different types of spermatozoa was also shown in this study.

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Molecular identification of the trematode P. ichunensis stat. n. from lungs of siberian tigers justified reappraisal of Paragonimus westermani species complex
Anastasia N. Voronova, Konstantin S. Vainutis, Tatiana V. Tabakaeva, Mikhail V. Sapotsky, Nadezhda N. Kakareka, Yury G. Volkov, Irina V. Galkina, Mikhail Yu. Shchelkanov
Journal of Parasitic Diseases.2022; 46(3): 744. CrossRef

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Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi by Real-Time PCR with High Resolution Melting Analysis: Amornmas Kongklieng, Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Viraphong Lulitanond, Pusadee Sri-Aroon, Yanin Limpanont, Wanchai Maleewong; Korean J Parasitol 2013;51(6):651-656.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.651

Abstract
PDF

Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were 84.5±0.07℃ and 85.7±0.07℃, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts.

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Subash C.B. Gopinath, Thean-Hock Tang, Yeng Chen, Marimuthu Citartan, Thangavel Lakshmipriya
Biosensors and Bioelectronics.2014; 60: 332. CrossRef

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Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P: Tongjit Thanchomnang, Pewpan M. Intapan, Chairat Tantrawatpan, Viraphong Lulitanond, Sudchit Chungpivat, Piyanan Taweethavonsawat, Worasak Kaewkong, Oranuch Sanpool, Penchom Janwan, Wej Choochote, Wanchai Maleewong; Korean J Parasitol 2013;51(6):645-650.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.645

Abstract
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A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.

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Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction
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Modulation of Antibody Responses against Gnathostoma spinigerum in Mice Immunized with Crude Antigen Formulated in CpG Oligonucleotide and Montanide ISA720: Pewpan M. Intapan, Chakrit Hirunpetcharat, Churairat Kularbkaew, Wiboonchai Yutanawiboonchai, Penchom Janwan, Wanchai Maleewong; Korean J Parasitol 2013;51(6):637-644.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.637

Abstract
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This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.

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Evaluation of immunodiagnostic tests for human gnathostomiasis using different antigen preparations of Gnathostoma spinigerum larvae against IgE, IgM, IgG, IgG1‐4 and IgG1 patterns of post‐treated patients
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Tropical Medicine & International Health.2021; 26(12): 1634. CrossRef

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Susceptibility of Laboratory Rodents to Trichinella papuae: Lakkhana Sadaow, Pewpan M. Intapan, Thidarut Boonmars, Nimit Morakote, Wanchai Maleewong; Korean J Parasitol 2013;51(6):629-632.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.629

Abstract
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Members of the genus Trichinella are small nematodes that can infect a wide range of animal hosts. However, their infectivity varies depending on the parasite and host species combination. In this study, we examined the susceptibility of 4 species of laboratory rodents, i.e., mice, rats, hamsters, and gerbils to Trichinella papuae, an emerging non-encapsulated Trichinella species. Trichinella spiralis and Trichinella pseudospiralis were also included in this study for comparison. Fifteen animals of each rodent species were infected orally with 100 muscle larvae of each Trichinella species. Intestinal worm burden was determined at day 6 and 10 post-inoculation (PI). The numbers of muscle larvae were examined at day 45 PI. The reproductive capacity index (RCI) of the 3 Trichinella species in different rodent hosts was determined. By day 6 PI, 33.2-69.6% of the inoculated larvae of the 3 Trichinella species became adult worms in the small intestines of the host animals. However, in rats, more than 96% of adult worms of all 3 Trichinella species were expelled from the gut by day 10 PI. In gerbils, only 4.8-18.1% of adult worms were expelled by day 10 PI. In accordance with the intestinal worm burden and the persistence of adults, the RCI was the highest in gerbils with values of 241.5±41.0 for T. papuae, 432.6±48 for T. pseudospiralis, and 528.6±20.6 for T. spiralis. Hamsters ranked second and mice ranked third in susceptibility in terms of the RCI, Rats yielded the lowest parasite RCI for all 3 Trichinella species. Gerbils may be an alternative laboratory animal for isolation and maintenance of Trichinella spp.

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