Ticks are important vectors of numerous zoonotic pathogens, yet integrated molecular surveillance of wildlife hosts and their parasitizing ticks remains limited in Korea. In this study, we investigated the occurrence of major tick-borne bacterial pathogens in wildlife and their associated ticks. Blood samples were collected from Korean water deer (Hydropotes inermis), raccoon dogs (Nyctereutes procyonoides), and wild boars (Sus scrofa), and a total of 246 ticks were obtained from these 34 animals. Molecular analyses identified multiple bacterial pathogens, including Anaplasma phagocytophilum, Ehrlichia canis, Bartonella schoenbuchensis, Rickettsia raoultii, Rickettsia monacensis, and Candidatus Rickettsia longicornii. Notably, E. canis was detected for the first time in blood samples from raccoon dogs and wild boars in Korea, and B. schoenbuchensis was identified in ticks for the first time in the country. In addition, R. monacensis exhibited a remarkably high minimum infection rate in Ixodes nipponensis, and all positive ticks were collected from raccoon dogs. Strikingly, Ca. R. longicornii was detected at a very high prevalence (94.1%) in wildlife blood samples, suggesting extensive circulation among wildlife hosts in the study area. These findings indicate that wildlife and their parasitic ticks may serve as important reservoirs of diverse tick-borne bacteria in Korea and highlight the importance of continuous molecular surveillance within a One Health framework.

This study surveyed the current status of intestinal parasite infections in Korean dog fecal samples. A total of 367 fecal samples were collected from the northern (Seoul and Gyeonggi-do), central (Chungcheong-do), and southern (Gyeongsang-do) regions and analyzed using the saturated sodium nitrate flotation technique and the nucleotide sequences of 18S rRNA. Six species of intestinal parasites were detected using the flotation technique. Among them, helminth eggs detected included Toxocara canis (6.0%), Toxascaris leonina (1.1%), Trichuris vulpis (6.8%), Ancylostoma caninum (2.7%), and Spirometra sp. (1.1%). Additionally, Cystoisospora sp. (7.6%) oocysts were also detected. The prevalence of intestinal parasite infections was higher in shelter dogs than in pet dogs. Molecular genetic assays revealed the gdh and 18S rRNA genes of Giardia duodenalis (type D) in 4.9% of fecal samples. To the best of our knowledge, 18S rRNA genes of Cryptosporidium canis were identified in 1.9% of fecal samples for the first time in Korea. These findings provide an overview of the current status of intestinal parasite infections in fecal samples of dogs from Korea and can be helpful in the surveillance of zoonotic parasite infections related to dogs.