Parasites, Hosts and Diseases

"bile duct"

Original Article

Bile Ductal Transcriptome Identifies Key Pathways and Hub Genes in Clonorchis sinensis-Infected Sprague-Dawley Rats: Won Gi Yoo, Jung-Mi Kang, Huong Giang L?, Jhang Ho Pak, Sung-Jong Hong, Woon-Mok Sohn, Byoung-Kuk Na; Korean J Parasitol 2020;58(5):513-525.
Published online October 22, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.5.513

Abstract
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Clonorchis sinensis is a food-borne trematode that infects more than 15 million people. The liver fluke causes clonorchiasis and chronical cholangitis, and promotes cholangiocarcinoma. The underlying molecular pathogenesis occurring in the bile duct by the infection is little known. In this study, transcriptome profile in the bile ducts infected with C. sinensis were analyzed using microarray methods. Differentially expressed genes (DEGs) were 1,563 and 1,457 at 2 and 4 weeks after infection. Majority of the DEGs were temporally dysregulated at 2 weeks, but 519 DEGs showed monotonically changing expression patterns that formed seven distinct expression profiles. Protein-protein interaction (PPI) analysis of the DEG products revealed 5 sub-networks and 10 key hub proteins while weighted co-expression network analysis (WGCNA)-derived gene-gene interaction exhibited 16 co-expression modules and 13 key hub genes. The DEGs were significantly enriched in 16 Kyoto Encyclopedia of Genes and Genomes pathways, which were related to original systems, cellular process, environmental information processing, and human diseases. This study uncovered a global picture of gene expression profiles in the bile ducts infected with C. sinensis, and provided a set of potent predictive biomarkers for early diagnosis of clonorchiasis.

Citations

Citations to this article as recorded by

Exploratory study on the role of Clonorchis sinensis infection in promoting cholangiocarcinoma progression
Shitao Li, Yiqi Jiang, Jun Kawanokuchi, Xueling Deng, Yuhong Wu, Yu Chen, Lixia Zeng, Ganghuan Deng, Damian Li, Tingzheng Zhan, Dengyu Liu, Ning Ma, Zeli Tang
Parasites & Vectors.2025;[Epub] CrossRef
Liver Fluke-Derived Molecules Accelerate Skin Repair Processes in a Mouse Model of Type 2 Diabetes Mellitus
Anna Kovner, Yaroslav Kapushchak, Oxana Zaparina, Dmitry Ponomarev, Maria Pakharukova
International Journal of Molecular Sciences.2024; 25(22): 12002. CrossRef
Comparative liver transcriptome analysis in hamsters infected with food-borne trematodes Opisthorchis felineus, Opisthorchis viverrini, or Clonorchis sinensis
Ekaterina A. Lishai, Oxana G. Zaparina, Yaroslav K. Kapushchak, Banchob Sripa, Sun-Jong Hong, Guofeng Cheng, Maria Y. Pakharukova, Feng Xue
PLOS Neglected Tropical Diseases.2024; 18(12): e0012685. CrossRef
Current status of Clonorchis sinensis and clonorchiasis in Korea: epidemiological perspectives integrating the data from human and intermediate hosts
Won Gi Yoo, Woon-Mok Sohn, Byoung-Kuk Na
Parasitology.2022; 149(10): 1296. CrossRef
Transcriptomic profiling of three-dimensional cholangiocyte spheroids long term exposed to repetitive Clonorchis sinensis excretory-secretory products
Jung-Woong Kim, Junyeong Yi, Jinhong Park, Ji Hoon Jeong, Jinho Kim, Jihee Won, Seok Chung, Tong-Soo Kim, Jhang Ho Pak
Parasites & Vectors.2021;[Epub] CrossRef

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Case Reports

A Case of Fasciola hepatica Infection Mimicking Cholangiocarcinoma and ITS-1 Sequencing of the Worm: Bong Kyun Kang, Bong-Kwang Jung, Yoon Suk Lee, In Kyeom Hwang, Hyemi Lim, Jaeeun Cho, Jin-Hyeok Hwang, Jong-Yil Chai; Korean J Parasitol 2014;52(2):193-196.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.193

Abstract
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Fascioliasis is a zoonotic infection caused by Fasciola hepatica or Fasciola gigantica. We report an 87-year-old Korean male patient with postprandial abdominal pain and discomfort due to F. hepatica infection who was diagnosed and managed by endoscopic retrograde cholangiopancreatography (ERCP) with extraction of 2 worms. At his first visit to the hospital, a gallbladder stone was suspected. CT and magnetic retrograde cholangiopancreatography (MRCP) showed an intraductal mass in the common bile duct (CBD) without proximal duct dilatation. Based on radiological findings, the presumed diagnosis was intraductal cholangiocarcinoma. However, in ERCP which was performed for biliary decompression and tissue diagnosis, movable materials were detected in the CBD. Using a basket, 2 living leaf-like parasites were removed. The worms were morphologically compatible with F. hepatica. To rule out the possibility of the worms to be another morphologically close species, in particular F. gigantica, 1 specimen was processed for genetic analysis of its ITS-1 region. The results showed that the present worms were genetically identical (100%) with F. hepatica but different from F. gigantica.

Citations

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A Curious Culprit Behind Biliary Pus: What’s the Diagnosis?
Partha Pal, Uday Kumar Marri, D. Nageshwar Reddy
Gastroenterology.2026; 170(1): 21. CrossRef
Parasitic Diseases of Upper Gastrointestinal Tract
Heung Up Kim
The Korean Journal of Helicobacter and Upper Gastrointestinal Research.2024; 24(2): 127. CrossRef
Global distribution of zoonotic digenetic trematodes: a scoping review
Yue Hu, Rong-Jian Zhan, Shi-Lin Lu, Yi-Yang Zhang, Min-Yu Zhou, Hui Huang, Ding-Ding Wang, Tao Zhang, Zi-Xin Huang, Yun-Fei Zhou, Zhi-Yue Lv
Infectious Diseases of Poverty.2024;[Epub] CrossRef
General overview of the current status of human foodborne trematodiasis
Jong-Yil Chai, Bong-Kwang Jung
Parasitology.2022; 149(10): 1262. CrossRef
Phylogenetic Characteristics of Fasciola hepatica Isolated from a Korean Patient
Mi Jin Jeong, Jae Kyun Park, Hak Sun Yu
The Korean Journal of Parasitology.2022; 60(5): 367. CrossRef
Fasciola Hepatica Induced Hepatic Abscess Treated with Triclabendazole
Hyun Joon Park, Gil-Soon Choi, Minjung Jung, Sang Uk Lee
The Korean Journal of Gastroenterology.2021; 77(1): 39. CrossRef
Oxysterols of helminth parasites and pathogenesis of foodborne hepatic trematodiasis caused by Opisthorchis and Fasciola species
Nuno Vale, Maria João Gouveia, Fátima Gärtner, Paul J Brindley
Parasitology Research.2020; 119(5): 1443. CrossRef
Prolonged antibiotic use leading to Clostridium difficile colitis in an ill returned traveller with acute fascioliasis
Dylan Kain, Avinash N Mukkala, Andrea K Boggild
Journal of Travel Medicine.2018;[Epub] CrossRef
A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea
Hyo-Seok Kim, Joo-Yeon Kong, Jong-Hyun Kim, Seong-Chan Yeon, Il-Hwa Hong
The Korean Journal of Parasitology.2018; 56(4): 375. CrossRef
F-18-FDG PET/BT’de kolanjiosellüler kanseri taklit eden Fasciola hepatica vakası
Hüseyin KAÇMAZ, Elif Tuğba TUNCEL, Berat EBİK, Feyzullah UÇMAK, Muhsin KAYA, Kendal YALÇIN
Endoskopi Gastrointestinal.2018; 26(2): 69. CrossRef
Unexpected hosts: imaging parasitic diseases
Pablo Rodríguez Carnero, Paula Hernández Mateo, Susana Martín-Garre, Ángela García Pérez, Lourdes del Campo
Insights into Imaging.2017; 8(1): 101. CrossRef
Acute biliary obstruction with cholestasis caused by Fasciola hepatica in a patient travelled to Turcmenistan (a case report and literature review)
Alexander M. Bronshteyn, N. A Malyshev, L. V Fedianina, I. V Davydova
Epidemiology and Infectious Diseases.2016; 21(2): 92. CrossRef
Monitoring of Fasciola Species Contamination in Water Dropwort by COX1 Mitochondrial and ITS-2 rDNA Sequencing Analysis
In-Wook Choi, Hwang-Yong Kim, Juan-Hua Quan, Jae-Gee Ryu, Rubing Sun, Young-Ha Lee
The Korean Journal of Parasitology.2015; 53(5): 641. CrossRef
Endoscopic Extraction of Biliary Fascioliasis Diagnosed Using Intraductal Ultrasonography in a Patient with Acute Cholangitis
Ji Su Ha, Hyun Jong Choi, Jong Ho Moon, Yun Nah Lee, Jae Woong Tae, Moon Han Choi, Tae Hoon Lee, Sang-Woo Cha
Clinical Endoscopy.2015; 48(6): 579. CrossRef
Prevalence of Fasciola spp. from cattle in slaughterhouse by macroscopic examination
Bae-Keun Park, Eui-Ju Hong, Si-Yun Ryu, Bae-Dong Jung, Ji-Min Kim, Hyeon-Cheol Kim
Korean Journal of Veterinary Service.2015; 38(4): 227. CrossRef
Fasciola hepatica in Snails Collected from Water-Dropwort Fields using PCR
Hwang-Yong Kim, In-Wook Choi, Yeon-Rok Kim, Juan-Hua Quan, Hassan Ahmed Hassan Ahmed Ismail, Guang-Ho Cha, Sung-Jong Hong, Young-Ha Lee
The Korean Journal of Parasitology.2014; 52(6): 645. CrossRef

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A Case of Biliary Fascioliasis by Fasciola gigantica in Turkey: Vedat Goral, Senem Senturk, Omer Mete, Mutallib Cicek, Berat Ebik, Be?ir Kaya; Korean J Parasitol 2011;49(1):65-68.
Published online March 18, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.1.65

Abstract
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A case of Fasciola gigantica-induced biliary obstruction and cholestasis is reported in Turkey. The patient was a 37- year-old woman, and suffered from icterus, ascites, and pain in her right upper abdominal region. A total of 7 living adult flukes were recovered during endoscopic retrograde cholangiopancreatography (ERCP). A single dose of triclabendazole was administered to treat possible remaining worms. She was living in a village of southeast of Anatolia region and had sheeps and cows. She had the history of eating lettuce, mallow, dill, and parsley without washing. This is the first case of fascioliasis which was treated via endoscopic biliary extraction during ERCP in Turkey.

Citations

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Human and Animal Fascioliasis: Origins and Worldwide Evolving Scenario
Santiago Mas-Coma, M. Adela Valero, M. Dolores Bargues
Clinical Microbiology Reviews.2022;[Epub] CrossRef
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Sarah Gabriël, Pierre Dorny, Ganna Saelens, Veronique Dermauw
Foods.2022; 12(1): 142. CrossRef
Triclabendazole in the treatment of human fascioliasis: a review
Preetam Gandhi, Esther K Schmitt, Chien-Wei Chen, Sanjay Samantray, Vinay Kumar Venishetty, David Hughes
Transactions of The Royal Society of Tropical Medicine and Hygiene.2019; 113(12): 797. CrossRef
Fasciola hepatica in the common bile duct: spyglass visualization and endoscopic extraction
Edson Guzmán Calderón, Augusto Vera Calderón, Ramiro Díaz Ríos, Ronald Arcana López, Edgar Alva Alva
Revista Española de Enfermedades Digestivas.2018;[Epub] CrossRef
Human fascioliasis by Fasciola hepatica: the first case report in Nepal
Ranjit Sah, Shusila Khadka, Mohan Khadka, Dipesh Gurubacharya, Jeevan Bahadur Sherchand, Keshab Parajuli, Niranjan Prasad Shah, Hari Prasad Kattel, Bharat Mani Pokharel, Basista Rijal
BMC Research Notes.2017;[Epub] CrossRef
Biliary Fasciola gigantica infestation in a nonendemic area — An intraoperative surprise
Prema Menon, Amit Kumar Sinha, Katragadda Lakshmi Narasimha Rao, Sumeeta Khurana, Sadhana Lal, Babu Ram Thapa
Journal of Pediatric Surgery.2015; 50(11): 1983. CrossRef
Molecular identification ofFasciolaspp. (Digenea: Platyhelminthes) in cattle from Vietnam
S. Nguyen, S. Amer, M. Ichikawa, T. Itagaki, Y. Fukuda, Y. Nakai
Parasite.2012; 19(1): 85. CrossRef

10,591 View
86 Download
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Original Articles

Single Nucleotide Polymorphisms of Cytokine Genes are Associated with Fibrosis of the Intrahepatic Bile Duct Wall in Human Clonorchiasis: Byung-Suk Chung, Jeong-Keun Lee, Min-Ho Choi, Myoung Hee Park, Dongil Choi, Sung-Tae Hong; Korean J Parasitol 2009;47(2):145-151.
Published online May 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.145

Abstract
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This study examined the association of cytokine gene polymorphisms with intrahepatic bile duct wall fibrosis in human clonorchiasis. A total of 240 residents in Heilongjiang, China underwent ultrasonography, blood sampling, and stool examination. Single nucleotide polymorphism (SNP) sites for IFN-γ (+874 T/A), IL-10 (-1,082 G/A, -819 C/T, -592 C/A), TNF-α (-308 G/A), and TGF-β1 (codon 10 T/C, codon 25 G/C) genes were observed with the TaqMan allelic discrimination assay. No significant correlation was observed between individual cytokine gene polymorphisms and intrahepatic duct dilatation (IHDD). Among individuals with clonorchiasis of moderate intensity, the incidence of IHDD was high in those with IFN-γ intermediate-producing genotype, +874AT (80.0%, P = 0.177), and in those with TNF-α low-producing genotype, -308GG (63.0%, P = 0.148). According to the combination of IFN-γ and TNF-α genotypes, the risks for IHDD could be stratified into high (intermediate-producing IFN-γ and low producing TNF-α), moderate, and low (low-producing IFN-γ and high producing TNF-α) risk groups. The incidence of IHDD was significantly different among these groups (P = 0.022): 88.9% (odds ratio, OR = 24.0) in high, 56.5% (OR = 3.9) in moderate, and 25.0% (OR = 1) in low risk groups. SNP of IFN-γ and TNF-α genes may contribute to the modulation of fibrosis in the intrahepatic bile duct wall in clonorchiasis patients.

Citations

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Risk Factors of Clonorchis sinensis Human Infections in Endemic Areas, Haman-Gun, Republic of Korea: A Case-Control Study
Sang-Eun Lee, Hee-Eun Shin, Myoung-Ro Lee, Yang-Hee Kim, Shin-Hyeong Cho, Jung-Won Ju
The Korean Journal of Parasitology.2020; 58(6): 647. CrossRef
The impact of cytokine gene polymorphisms on Epstein–Barr virus infection outcome in pediatric liver transplant recipients
Beata Kasztelewicz, Irena Jankowska, Joanna Pawłowska, Joanna Teisseyre, Katarzyna Dzierżanowska-Fangrat
Journal of Clinical Virology.2012; 55(3): 226. CrossRef
Clinical relevance of the interleukin 10 promoter polymorphisms in Chinese Han patients with major trauma: genetic association studies
Ling Zeng, Wei Gu, Kehong Chen, Dongpo Jiang, Lianyang Zhang, Dingyuan Du, Ping Hu, Qing Liu, Suna Huang, Jianxin Jiang
Critical Care.2009;[Epub] CrossRef

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Changes in Sonographic Findings after Treatment of Patients with Clonorchiasis in a Heavy Endemic Area: Dongil Choi, Yong Hwan Jeon, Geun-Chan Lee, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2009;47(1):19-23.
Published online March 12, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.1.19

Abstract
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We measured changes in sonographic findings of patients with clonorchiasis after a treatment in a highly endemic area. A total of 347 residents showed positive stool results for Clonorchis sinensis eggs in a village in northeastern China, and were treated with praziquantel. Of them, 132 patients underwent abdominal sonography both before and 1 year after treatment, and the changes in sonographic findings of 83 cured subjects were compared. Diffuse dilatation of intrahepatic bile ducts (DDIHD) was found in 82 patients (98.2%) before and 80 (96.4%) after treatment, which was improved in 3, aggravated in 1, and unchanged in 79 patients. Increased periductal echogenicity (IPDE) was observed in 42 patients (50.6%) before and 45 (54.2%) after treatment, which was improved in 5, aggravated in 8, and unchanged in 70 patients. Floating echogenic foci in the gallbladder (FEFGB) was detected in 32 patients (38.6%) before and 17 (20.5%) after treatment, which was improved in 20, aggravated in 5, and unchanged in 58 patients. Improvement of FEFGB only was statistically significantly (P = 0.004). The present results confirm that DDIHD and IPDE persist but FEFGB decreases significantly at 1 year after treatment. In a heavy endemic area, the sonographic finding of FEFGB may suggest active clonorchiasis 1 year after treatment.

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