Parasites, Hosts and Diseases

"molecular phylogeny"

Brief Communication

Occurrence and Molecular Identification of Microcotyle sebastis Isolated from Fish Farms of the Korean Rockfish, Sebastes schlegelii: Jun-Young Song, Keun-Yong Kim, Seo-Woo Choi; Korean J Parasitol 2021;59(1):89-95.
Published online February 19, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.1.89

Abstract
PDF

Microcotyle sebastis is a gill monogenean ectoparasite that causes serious problems in the mariculture of the Korean rockfish, Sebastes schlegelii. In this study, we isolated the parasite from fish farms along the coasts of Tongyeong, South Korea in 2016, and characterized its infection, morphology and molecular phylogeny. The prevalence of M. sebastis infection during the study period ranged from 46.7% to 96.7%, and the mean intensity was 2.3 to 31.4 ind./fish, indicating that the fish was constantly exposed to parasitic infections throughout the year. Morphological observations under light and scanning electron microscopes of the M. sebastis isolates in this study showed the typical characteristics of the anterior prohaptor and posterior opisthaptor of monogenean parasites. In phylogenetic trees reconstructed using the nuclear 28S ribosomal RNA gene and the mitochondrial cytochrome c oxidase I gene (cox1), they consistently clustered together with their congeneric species, and showed the closest phylogenetic relationships to M. caudata and M. kasago in the cox1 tree.

Citations

Citations to this article as recorded by

Redescription of Microcotyle elegans (Polyopisthocotyla: Microcotylidae) from Scombrops boops (Perciformes: Scombropidae) off Japanese Waters
Yusuke Kamio, Masato Nitta
Species Diversity.2025; 30(2): 111. CrossRef
Microscopic findings of host–parasite interaction between the Korean rockfish, Sebastes schlegelii, and the monogenean ectoparasite, Microcotyle sebastis
Sang Phil Shin, Seokryel Kim
Parasites, Hosts and Diseases.2024; 62(4): 490. CrossRef
The genus Microcotyle in Mediterranean scorpaenoids (Teleostei), including the description of Microcotyle merche n. sp. from Helicolenus dactylopterus (Delaroche, 1809)
M. Víllora-Montero, A. Pérez-del-Olmo, M. Valmaseda-Angulo, J.A. Raga, F.E. Montero
Journal of Helminthology.2023;[Epub] CrossRef
Correlation of endemicity between monogenean trematode, Microcotyle sebastis, and parasitic copepods in Korean rockfish, Sebastes schlegelii, from a fish farm in Tongyeong-si, Gyeongsangnam-do, Korea
Won-Sik Woo, Gyoungsik Kang, Kyung-Ho Kim, Ha-Jeong Son, Min-Young Sohn, Chan-Il Park
Parasites, Hosts and Diseases.2023; 61(3): 332. CrossRef
Assessment of Salinomycin’s Potential to Treat Microcotyle sebastis in Korean Rockfish (Sebastes schlegelii)
Won-Sik Woo, Sang Hee Shim, Gyoungsik Kang, Kyung-Ho Kim, Ha-Jeong Son, Min-Young Sohn, Seungjin Lee, Jaekyeong Kim, Jung-Soo Seo, Mun-Gyeong Kwon, Do-Hyung Kim, Chan-Il Park
Animals.2023; 13(20): 3233. CrossRef
Description of a New Species, Microcotyle pacinkar n. sp. (Monogenea: Microcotylidae), Parasitic on Gills of Sebastes taczanowskii (Sebastidae) from off Usujiri, Hokkaido, Northern Japan
Yusuke Kamio, Masato Nitta
Species Diversity.2023; 28(2): 263. CrossRef
Kannaphallus leptosomus n. sp. (Monogenea: Heteraxinidae: Cemocotylinae) parasitizing Scyris indica (Carangiformes: Carangidae) from Malaysia
Masato Nitta, Yusuke Kondo, Susumu Ohtsuka, Ahmad Syazni Kamarudin, Norshida Ismail
Systematic Parasitology.2022; 99(5): 587. CrossRef

5,530 View
115 Download
5 Web of Science
Crossref

Original Articles

Molecular Phylogenetics of Centrocestus formosanus (Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand: Chalobol Wongsawad, Pheravut Wongsawad, Kom Sukontason, Worawit Maneepitaksanti, Nattawadee Nantarat; Korean J Parasitol 2017;55(1):31-37.
Published online February 28, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.1.31

Abstract
PDF

This study aimed to investigate the morphology and reconstruct the phylogenetic relationships of Centrocestus formosanus originating from 5 species of freshwater fish, i.e., Esomus metallicus, Puntius brevis, Anabas testudineus, Parambassis siamensis, and Carassius auratus, in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adult C. formosanus from day 5 after infection in chicks. C. formosanus originated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those from C. auratus which revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability of C. formosanus also occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity of C. formosanus with 34 circumoral spines originating from C. auratus in the future.

Citations

Citations to this article as recorded by

Loop‐Mediated Isothermal Amplification Combined With Lateral‐Flow Dipstick for Detection of Centrocestus formosanus in Ornamental Fish
Metawee Sabaijai, Thanawan Tejangkura, Thapana Chontananarth
Journal of Fish Diseases.2025;[Epub] CrossRef
Revision of Cryptogonimus Osborn, 1903 and Caecincola Marshall et Gilbert, 1905 (Digenea: Cryptogonimidae), supplemental description of Cryptogonimus chili Osborn, 1903, and description of a new species of Caecincola infecting basses (Centrarchiformes: Ce
Triet N. Truong, Stephen S. Curran, Brandon L. Simcox, Alex P. Bybel, Stephen A. Bullard
Folia Parasitologica.2025;[Epub] CrossRef
Innovative molecular and immunological approaches of heterophyiasis infecting some Egyptian marketed fishes
Olfat A. Mahdy, Reem M. Ramadan, Mai A. Salem
BMC Veterinary Research.2024;[Epub] CrossRef
Multiplex PCR development for the simultaneous and rapid detection of two pathogenic flukes, Dactylogyrus spp. and Centrocestus formosanus, in ornamental fishes
Phonkawin Jaruboonyakorn, Thanawan Tejangkura, Thapana Chontananarth
Aquaculture.2022; 548: 737660. CrossRef
Efficacy of Acriflavine for controlling parasitic diseases in farmed Nile tilapia with emphasis on fish health, gene expression analysis, oxidative stress, and histopathological alterations
Mahmoud Abou-Okada, Huda O. AbuBakr, Azza Hassan, Shimaa Abdel-Radi, Samira H. Aljuaydi, Mohamed Abdelsalam, Engy Taha, Nehal A. Younis, Dalia A. Abdel-Moneam
Aquaculture.2021; 541: 736791. CrossRef
A review of molecular identification tools for the opisthorchioidea
Maureen Duflot, Thomas Setbon, Graziella Midelet, Thomas Brauge, Mélanie Gay
Journal of Microbiological Methods.2021; 187: 106258. CrossRef
Streptococcus, Centrocestus formosanus and Myxobolus tilapiae concurrent infections in farmed Nile tilapia (Oreochromis niloticus)
Alaa Eldin Eissa, Marwa M. Attia, Mamdouh Y. Elgendy, Gehad A. Ismail, Nader M. Sabry, Abdelbary Prince, Mahmoud A. Mahmoud, Ghada O. El-Demerdash, Mohamed Abdelsalam, Hassan I.M. Derwa
Microbial Pathogenesis.2021; 158: 105084. CrossRef
Molecular classification of rumen fluke eggs in fecal specimens from Suphanburi Province, Thailand, based on cytochrome C oxidase subunit 1
Sothorn Anucherngchai, Thapana Chontananarth, Thanawan Tejangkura, Chalobol Wongsawad
Veterinary Parasitology: Regional Studies and Reports.2020; 20: 100382. CrossRef
Infections of Digenetic Trematode Metacercariae in Wrestling Halfbeak, Dermogenys pusilla from Bangkok Metropolitan Region in Thailand
Laddawan Patarwut, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
The Korean Journal of Parasitology.2020; 58(1): 27. CrossRef
Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio) in Italy: A Window to a New Globalization-Derived Invasive Microorganism
Antonino Pace, Ludovico Dipineto, Serena Aceto, Maria Concetta Censullo, Maria Carmen Valoroso, Lorena Varriale, Laura Rinaldi, Lucia Francesca Menna, Alessandro Fioretti, Luca Borrelli
Animals.2020; 10(3): 456. CrossRef
The life cycle of a zoonotic parasite reassessed: Experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae)
Hudson A. Pinto, Nicole Q. Gonçalves, Danimar López-Hernandez, Eduardo A. Pulido-Murillo, Alan L. Melo, Petr Heneberg
PLOS ONE.2018; 13(4): e0194161. CrossRef
Prevalence of Centrocestus formosanus Metacercariae in Ornamental Fish from Chiang Mai, Thailand, with Molecular Approach Using ITS2
Atcharaphan Wanlop, Chalobol Wongsawad, Pongphol Prattapong, Pheravut Wongsawad, Thapana Chontananarth, Jong-Yil Chai
The Korean Journal of Parasitology.2017; 55(4): 445. CrossRef

9,863 View
196 Download
14 Web of Science
Crossref

Complete Mitochondrial Genome of Haplorchis taichui and Comparative Analysis with Other Trematodes: Dongmin Lee, Seongjun Choe, Hansol Park, Hyeong-Kyu Jeon, Jong-Yil Chai, Woon-Mok Sohn, Tai-Soon Yong, Duk-Young Min, Han-Jong Rim, Keeseon S. Eom; Korean J Parasitol 2013;51(6):719-726.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.719

Abstract
PDF

Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.

Citations

Citations to this article as recorded by

The complete mitochondrial genome of Aspidogaster ijimai (Platyhelminthes: Trematoda: Aspidogastrea): gene content and phylogenetic inference
D. A. Solodovnik, D. M. Atopkin, A. A. Semenchenko, M. Urabe, S. G. Sokolov
Invertebrate Zoology.2025; 22(3): 411. CrossRef
Trematode species diversity in the faucet snail, Bithynia tentaculata at the western edge of its native distribution, in Ireland
A. Faltýnková, K. O’Dwyer, C. Pantoja, D. Jouet, K. Skírnisson, O. Kudlai
Journal of Helminthology.2024;[Epub] CrossRef
Black spot diseases in seven commercial fish species from the English Channel and the North Sea: infestation levels, identification and population genetics of Cryptocotyle spp.
Maureen Duflot, Pierre Cresson, Maéva Julien, Léa Chartier, Odile Bourgau, Marialetizia Palomba, Simonetta Mattiucci, Graziella Midelet, Mélanie Gay
Parasite.2023; 30: 28. CrossRef
A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences
D. M. Atopkin, A. A. Semenchenko, D. A. Solodovnik, Y. I. Ivashko
Journal of Helminthology.2023;[Epub] CrossRef
Mitophylogenomics of the zoonotic fluke Echinostoma malayanum confirms it as a member of the genus Artyfechinostomum Lane, 1915 and illustrates the complexity of Echinostomatidae systematics
Linh Thi Khanh Pham, Weerachai Saijuntha, Scott P. Lawton, Thanh Hoa Le
Parasitology Research.2022; 121(3): 899. CrossRef
Characterization of complete mitochondrial genome and ribosomal operon forCarassotrema koreanumPark, 1938 (Digenea: Haploporidae) by means of next-generation sequencing data
Y.I. Ivashko, A.A. Semenchenko, D.A. Solodovnik, D.M. Atopkin
Journal of Helminthology.2022;[Epub] CrossRef
Characterization of the complete mitochondrial genomes of Diplodiscus japonicus and Diplodiscus mehari (Trematoda: Diplodiscidae): Comparison with the members of the superfamily Paramphistomoidea and phylogenetic implication
Qi An, Yang-Yuan Qiu, Yan Lou, Yan Jiang, Hong-Yu Qiu, Zhong-Huai Zhang, Ben Li, Ai-Hui Zhang, Wei Wei, Ying-Yu Chen, Jun-Feng Gao, Chun-Ren Wang
International Journal for Parasitology: Parasites and Wildlife.2022; 19: 9. CrossRef
The complete mitogenome of the Asian lung fluke Paragonimus skrjabini miyazakii and its implications for the family Paragonimidae (Trematoda: Platyhelminthes)
Thanh Hoa Le, Khue Thi Nguyen, Linh Thi Khanh Pham, Huong Thi Thanh Doan, Takeshi Agatsuma, David Blair
Parasitology.2022; 149(13): 1709. CrossRef
Gene Duplication and Gain in the Trematode Atriophallophorus winterbourni Contributes to Adaptation to Parasitism
Natalia Zajac, Stefan Zoller, Katri Seppälä, David Moi, Christophe Dessimoz, Jukka Jokela, Hanna Hartikainen, Natasha Glover, Dennis Lavrov
Genome Biology and Evolution.2021;[Epub] CrossRef
First next-generation sequencing data for Haploporidae (Digenea: Haploporata): characterization of complete mitochondrial genome and ribosomal operon for Parasaccocoelium mugili Zhukov, 1971
Dmitry M. Atopkin, Alexander A. Semenchenko, Daria A. Solodovnik, Yana I. Ivashko, Kirill A. Vinnikov
Parasitology Research.2021; 120(6): 2037. CrossRef
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel
Maureen Duflot, Mélanie Gay, Graziella Midelet, Per Walter Kania, Kurt Buchmann
Parasitology Research.2021; 120(10): 3417. CrossRef
Discovery of Carcinogenic Liver Fluke Metacercariae in Second Intermediate Hosts and Surveillance on Fish-Borne Trematode Metacercariae Infections in Mekong Region of Myanmar
Ei Ei Phyo Myint, Amornpun Sereemaspun, Joacim Rocklöv, Choosak Nithikathkul
International Journal of Environmental Research and Public Health.2020; 17(11): 4108. CrossRef
Characterization of the mitochondrial genome sequences of the liver fluke Amphimerus sp. (Trematoda: Opisthorchiidae) from Ecuador and phylogenetic implications
Jun Ma, Jun-Jun He, Cheng-Yan Zhou, Miao-Miao Sun, William Cevallos, Hiromu Sugiyama, Xing-Quan Zhu, Manuel Calvopiña
Acta Tropica.2019; 195: 90. CrossRef
The complete mitochondrial genome of Paragonimus ohirai (Paragonimidae: Trematoda: Platyhelminthes) and its comparison with P. westermani congeners and other trematodes
Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, Takeshi Agatsuma, David Blair
PeerJ.2019; 7: e7031. CrossRef
The Complete Mitochondrial Genome of the Caecal Fluke of Poultry, Postharmostomum commutatum, as the First Representative from the Superfamily Brachylaimoidea
Yi-Tian Fu, Yuan-Chun Jin, Guo-Hua Liu
Frontiers in Genetics.2019;[Epub] CrossRef
The complete mitochondrial genome of Orthocoelium streptocoelium (Digenea: Paramphistomidae) for comparison with other digeneans
Y.Y. Zhao, X. Yang, H.M. Chen, L.X. Wang, H.L. Feng, P.F. Zhao, L. Tan, W.Q. Lei, Y. Ao, M. Hu, R. Fang
Journal of Helminthology.2017; 91(2): 255. CrossRef
Molecular characterization and confocal laser scanning microscopic study of Pygidiopsis macrostomum (Trematoda: Heterophyidae) parasites of guppies Poecilia vivipara
J N Borges, V S Costa, C Mantovani, E Barros, E G N Santos, C L Mafra, C P Santos
Journal of Fish Diseases.2017; 40(2): 191. CrossRef
Molecular characterization of the unique Mesostephanus appendiculatus (Trematoda: Cyathocotylidae) by small ribosomal RNA from Egypt
Nasr M. El-Bahy, Eman K. Bazh, Shimaa S. Sorour, Nagwa M. Elhawary
Parasitology Research.2017; 116(4): 1129. CrossRef
Fishborne zoonotic heterophyid infections: An update
Jong-Yil Chai, Bong-Kwang Jung
Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef
Updated molecular phylogenetic data for Opisthorchis spp. (Trematoda: Opisthorchioidea) from ducks in Vietnam
Thanh Thi Ha Dao, Thanh Thi Giang Nguyen, Sarah Gabriël, Khanh Linh Bui, Pierre Dorny, Thanh Hoa Le
Parasites & Vectors.2017;[Epub] CrossRef
Mitochondrial genome of Ogmocotyle sikae and implications for phylogenetic studies of the Notocotylidae trematodes
Jun Ma, Jun-Jun He, Guo-Hua Liu, David Blair, Li-Zhi Liu, Yi Liu, Xing-Quan Zhu
Infection, Genetics and Evolution.2016; 37: 208. CrossRef
The complete mitochondrial genome of Metorchis orientalis (Trematoda: Opisthorchiidae): Comparison with other closely related species and phylogenetic implications
Lu Na, Jun-Feng Gao, Guo-Hua Liu, Xue Fu, Xin Su, Dong-Mei Yue, Yuan Gao, Yan Zhang, Chun-Ren Wang
Infection, Genetics and Evolution.2016; 39: 45. CrossRef
Epidemiological and molecular data on heterophyid trematode metacercariae found in the muscle of grey mullets (Osteichthyes: Mugilidae) from Sardinia (western Mediterranean Sea)
Simonetta Masala, Maria Cristina Piras, Daria Sanna, Jong-Yil Chai, Bong-Kwang Jung, Woon-Mok Sohn, Giovanni Garippa, Paolo Merella
Parasitology Research.2016; 115(9): 3409. CrossRef
Complete mitochondrial genome analysis of Clinostomum complanatum and its comparison with selected digeneans
Lu Chen, Yan Feng, Hong-Mei Chen, Li-Xia Wang, Han-Li Feng, Xin Yang, Mudassar-Niaz Mughal, Rui Fang
Parasitology Research.2016; 115(8): 3249. CrossRef
Complete Mitochondrial Genome of Echinostoma hortense (Digenea: Echinostomatidae)
Ze-Xuan Liu, Yan Zhang, Yu-Ting Liu, Qiao-Cheng Chang, Xin Su, Xue Fu, Dong-Mei Yue, Yuan Gao, Chun-Ren Wang
The Korean Journal of Parasitology.2016; 54(2): 173. CrossRef
Characterization of the complete mitochondrial genome sequence of Homalogaster paloniae (Gastrodiscidae, Trematoda) and comparative analyses with selected digeneans
Xin Yang, Lixia Wang, Hanli Feng, Mingwei Qi, Zongze Zhang, Chong Gao, Chunqun Wang, Min Hu, Rui Fang, Chengye Li
Parasitology Research.2016; 115(10): 3941. CrossRef
A complete mitochondrial genome from Echinochasmus japonicus supports the elevation of Echinochasminae Odhner, 1910 to family rank (Trematoda: Platyhelminthes)
Thanh Hoa Le, Nga Thi Bich Nguyen, Khue Thi Nguyen, Huong Thi Thanh Doan, Do Trung Dung, David Blair
Infection, Genetics and Evolution.2016; 45: 369. CrossRef
Mitochondrial and nuclear ribosomal DNA dataset supports that Paramphistomum leydeni (Trematoda: Digenea) is a distinct rumen fluke species
Jun Ma, Jun-Jun He, Guo-Hua Liu, Dong-Hui Zhou, Jian-Zhi Liu, Yi Liu, Xing-Quan Zhu
Parasites & Vectors.2015;[Epub] CrossRef
Analysis of the complete Fischoederius elongatus (Paramphistomidae, Trematoda) mitochondrial genome
Xin Yang, Yunyang Zhao, Lixia Wang, Hanli Feng, Li Tan, Weiqiang Lei, Pengfei Zhao, Min Hu, Rui Fang
Parasites & Vectors.2015;[Epub] CrossRef
Dicrocoelium chinensis and Dicrocoelium dendriticum (Trematoda: Digenea) are distinct lancet fluke species based on mitochondrial and nuclear ribosomal DNA sequences
Guo-Hua Liu, Hong-Bin Yan, Domenico Otranto, Xing-Ye Wang, Guang-Hui Zhao, Wan-Zhong Jia, Xing-Quan Zhu
Molecular Phylogenetics and Evolution.2014; 79: 325. CrossRef

11,660 View
100 Download
Crossref

Mini Review

Molecular Phylogeny of Acanthamoeba: Hyun Hee Kong; Korean J Parasitol 2009;47(Suppl):S21.
Published online October 26, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.S.S21

Abstract
PDF

After morphological grouping of Acanthamoeba by Pussard and Pons, phylogeny of the genus has been always a big topic to the researchers. Because of the variability of morphological characteristics, unchangeable and stable characters have been investigated for phylogenic criteria. Isoenzyme and mitochondrial DNA RFLP (Mt DNA RFLP) analyses revealed different patterns among strains assigned to a same species. Therefore, these characteristics would be considered as tools for strain discrimination than species identification. The most recently developed and the most promising method is the sequence analysis of 18s ribosomal RNA coding DNA (18s rDNA). The phylogenic tree based on comparison of 18s rDNA sequences distinguished the 3 morphological groups of Acanthamoeba and divided them into 12 unique sequence types (T1-T12 genotypes). Most clinical and environmental isolates belonged to the morphological group II and the genotype T4. In the Republic of Korea, 2 strains of Acanthamoeba, YM-2 and YM-3, were first isolated from the environment in 1974. However, phylogenic identification of Korean Acanthamoeba isolates from human infections or the environment were tried from the late 1990s. By RFLP analysis or total sequence analysis of 18s rDNA revealed that almost all clinical isolates including the one from a suspicious granulomatous amebic encephalitis patient belonged to the genotype T4. A large number of environmental isolates from contact lens storage cases, tapped water, and ocean sediments also belonged to the genotype T4. Almost identical strain characteristics, such as Mt DNA RFLP pattern of environmental isolates, with the clinical isolates could make a simple conclusion that most environmental isolates might be a potential keratopathogen.

Citations

Citations to this article as recorded by

Insights Into the Genetic Diversity of Acanthamoeba Keratitis: A Clinical and Molecular Study
Vinil K. Kavya, Lakshmipathy Dhanurekha, Ramachandran Abirami, Ranganathan Sampathkumar, Agarwal S. Shweta, Appakkudal R. Anand, Sri B. Madhangi
Cornea.2025;[Epub] CrossRef
Epidemiology of and Genetic Factors Associated with Acanthamoeba Keratitis
Muhammad Ilyas, Fiona Stapleton, Mark D. P. Willcox, Fiona Henriquez, Hari Kumar Peguda, Binod Rayamajhee, Tasbiha Zahid, Constantinos Petsoglou, Nicole A. Carnt
Pathogens.2024; 13(2): 142. CrossRef
A Narrative Review of Acanthamoeba Isolates in Malaysia: Challenges in Infection Management and Natural Therapeutic Advancements
Mohammad Wisman Abdul Hamid, Roslaini Bin Abd Majid, Victor Fiezal Knight Victor Ernest, Nik Noorul Shakira Mohamed Shakrin, Firdaus Mohamad Hamzah, Mainul Haque
Cureus.2024;[Epub] CrossRef
Assessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer
Rohaya Abdul Halim, Hasseri Halim, Rosnani Hanim Mohd Hussain, Shafiq Aazmi, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar
Journal of Water and Health.2024; 22(12): 2289. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Shotgun Kinetic Target-Guided Synthesis Approach Enables the Discovery of Small-Molecule Inhibitors against Pathogenic Free-Living Amoeba Glucokinases
Mintesinot Kassu, Prakash T. Parvatkar, Jillian Milanes, Neil P. Monaghan, Chungsik Kim, Matthew Dowgiallo, Yingzhao Zhao, Ami H. Asakawa, Lili Huang, Alicia Wagner, Brandon Miller, Karissa Carter, Kayleigh F. Barrett, Logan M. Tillery, Lynn K. Barrett, I
ACS Infectious Diseases.2023; 9(11): 2190. CrossRef
Molecular detection of free-living amoebae from Namhangang (southern Han River) in Korea
Heekyoung Kang, Hae-Jin Sohn, Ga-Eun Seo, Gi-Sang Seong, A-Jeong Ham, A-Young Park, Suk-Yul Jung, Sang-Eun Lee, Shin-Hyeong Cho, Ho-Joon Shin
Scientific Reports.2020;[Epub] CrossRef
Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)
Stas Malavin, Lyubov Shmakova
European Journal of Protistology.2020; 73: 125671. CrossRef
Isolation and molecular characterization of Acanthamoeba from patients with keratitis in Spain
T. Martín-Pérez, A. Criado-Fornelio, J. Martínez, M.A. Blanco, I. Fuentes, J. Pérez-Serrano
European Journal of Protistology.2017; 61: 244. CrossRef
Free-living amoebae in the water resources of Iran: a systematic review
Ehsan Saburi, Toktam Rajaii, Asma Behdari, Mohammad Hasan Kohansal, Hossein Vazini
Journal of Parasitic Diseases.2017; 41(4): 919. CrossRef
Genotyping of Acanthamoeba spp. from water sources from Northwestern Iran
Ali Haniloo, Ali Pezeshki, Abbas Mahmmodzadeh, Elnaz Kadkhodamohammadi
Acta Parasitologica.2017;[Epub] CrossRef
Pathogenic free-living amoeba
Natalia Łanocha-Arendarczyk, Danuta Kosik-Bogacka, Katarzyna Galant, Wojciech Zaorski, Karolina Kot, Aleksandra Łanocha
Postępy Mikrobiologii - Advancements of Microbiology.2017; 56(1): 106. CrossRef
Metacommunity analysis of amoeboid protists in grassland soils
Anna Maria Fiore-Donno, Jan Weinert, Tesfaye Wubet, Michael Bonkowski
Scientific Reports.2016;[Epub] CrossRef
Genotypic, physiological, and biochemical characterization of potentially pathogenic Acanthamoeba isolated from the environment in Cairo, Egypt
Gihan Mostafa Tawfeek, Sawsan Abdel-Hamid Bishara, Rania Mohammad Sarhan, Eman ElShabrawi Taher, Amira ElSaady Khayyal
Parasitology Research.2016; 115(5): 1871. CrossRef
Incidence and molecular diversity of Acanthamoeba species isolated from public baths in Hungary
Csaba Kiss, Zsófia Barna, Márta Vargha, Júlia Katalin Török
Parasitology Research.2014; 113(7): 2551. CrossRef
Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
The Korean Journal of Parasitology.2013; 51(4): 401. CrossRef
Occurrence and characterization of Acanthamoeba similar to genotypes T4, T5, and T2/T6 isolated from environmental sources in Brasília, Federal District, Brazil
Daniella de Sousa Mendes Moreira Alves, Aline Silva Moraes, Nadjar Nitz, Mayara Gabriele Carvalho de Oliveira, Mariana Machado Hecht, Rodrigo Gurgel-Gonçalves, César Augusto Cuba Cuba
Experimental Parasitology.2012; 131(2): 239. CrossRef
Exploring the Unique N-Glycome of the Opportunistic Human Pathogen Acanthamoeba
Birgit Schiller, Georgia Makrypidi, Ebrahim Razzazi-Fazeli, Katharina Paschinger, Julia Walochnik, Iain B.H. Wilson
Journal of Biological Chemistry.2012; 287(52): 43191. CrossRef
Comparison of molecular diagnostic methods for the detection of Acanthamoeba spp. from clinical specimens submitted for keratitis
Krishna Khairnar, Gurdip S. Tamber, Filip Ralevski, Dylan R. Pillai
Diagnostic Microbiology and Infectious Disease.2011; 70(4): 499. CrossRef

19,260 View
194 Download
Crossref

Original Article

Molecular phylogeny of parasitic Platyhelminthes based on sequences of partial 28S rDNA D1 and mitochondrial cytochrome c oxidase subunit I: Soo-Ung Lee, Ha-Chung Chun, Sun Huh; Korean J Parasitol 2007;45(3):181-189.
Published online September 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.3.181

Abstract
PDF

The phylogenic relationships existing among 14 parasitic Platyhelminthes in the Republic of Korea were investigated via the use of the partial 28S ribosomal DNA (rDNA) D1 region and the partial mitochondrial cytochrome c oxidase subunit 1 (mCOI) DNA sequences. The nucleotide sequences were analyzed by length, G + C %, nucleotide differences and gaps in order to determine the analyzed phylogenic relationships. The phylogenic patterns of the 28S rDNA D1 and mCOI regions were closely related within the same class and order as analyzed by the PAUP 4.0 program, with the exception of a few species. These findings indicate that the 28S rDNA gene sequence is more highly conserved than are the mCOI gene sequences. The 28S rDNA gene may prove useful in studies of the systematics and population genetic structures of parasitic Platyhelminthes.

Citations

Citations to this article as recorded by

Ultrastructural and phylogenetic characterization of some trematode cercariae emerging from the freshwater ampullariid snail, Lanistes carinatus (Olivier, 1804)
Mohammed E. El-Zeiny, Ahmed M. Ghoneim, Ola A. Abu Samak, Abdel Aziz A. Khidr
Parasitology International.2025; 109: 103113. CrossRef
Seasonal dynamics and molecular phylogenetic studies on cercariae in Central Zone of Kashmir valley
Zahoor Ahmad Wani, Rafiq A. Shahardar, Kamal H. Bulbul, Idrees M. Allaie, Showkat A. Shah, Shabir A. Rather, Aiman Ashraf, Shahana Tramboo, Asif H. Khan, Shawky M Aboelhadid
PLOS One.2025; 20(7): e0325160. CrossRef
Deciphering the global genetic structure of Spirometra mansoni and S. erinaceieuropaei based on 28S ribosomal RNA: Insights into taxonomical revaluation and population dynamics
Anil Kumar Nehra, Rasmita Panda, Prem Sagar Maurya, Ansu Kumari, Aman Dev Moudgil
Parasitology Research.2025;[Epub] CrossRef
Identification of Hydatigera Species in Wildcats (Felis silvestris) from Central Spain
Pablo Matas-Méndez, Lorena Esteban-Sánchez, Francisco Ponce-Gordo, Marta Mateo-Barrientos
Animals.2025; 15(22): 3340. CrossRef
The infection of Cysticercus fasciolaris in natural rats (Rattus species) residing in human residence areas, Nakhon Si Thammarat, Thailand
Sunsaneeya Thaikoed, Watcharapong Mitsuwan, Kittipong Chaisiri, Noppharat Tanthanathipchai, Maria de Lourdes Pereira, Alok K. Paul, Veeranoot Nissapatorn, Phirabhat Saengsawang
Comparative Immunology, Microbiology and Infectious Diseases.2024; 107: 102152. CrossRef
Case report: Disseminated larval trematodiasis caused by Clinostomum marginatum in a green tree frog (Hyla cinerea)
Håkon Jones, Heather M.A. Fenton, Elizabeth J. Elsmo, Nicole M. Nemeth, Kayla B. Garrett, Christopher A. Cleveland, Michael J. Yabsley
Veterinary Parasitology: Regional Studies and Reports.2024; 52: 101051. CrossRef
Current status of intestinal parasitosis and microsporidiosis in industrialized countries: Results from a prospective study in France and Luxembourg
Maxime Moniot, Céline Nourrisson, Eloïse Bailly, Céline Lambert, Patricia Combes, Philippe Poirier, María Victoria Periago
PLOS Neglected Tropical Diseases.2024; 18(12): e0012752. CrossRef
Fatal infection with Versteria sp. in a muskrat, with implications for human health
Kevin D. Niedringhaus, Laken S. Ganoe, Matthew Lovallo, W. David Walter, Michael J. Yabsley, Justin D. Brown
Journal of Veterinary Diagnostic Investigation.2022; 34(2): 314. CrossRef
Expansion of Cyclophyllidea Biodiversity in Rodents of Qinghai-Tibet Plateau and the “Out of Qinghai-Tibet Plateau” Hypothesis of Cyclophyllideans
Yao-Dong Wu, Guo-Dong Dai, Li Li, D. Timothy J. Littlewood, John Asekhaen Ohiolei, Lin-Sheng Zhang, Ai-Min Guo, Yan-Tao Wu, Xing-Wei Ni, Nigus Abebe Shumuye, Wen-Hui Li, Nian-Zhang Zhang, Bao-Quan Fu, Yong Fu, Hong-Bin Yan, Wan-Zhong Jia
Frontiers in Microbiology.2022;[Epub] CrossRef
Morphological and molecular phylogenetic analysis of Bivesicula claviformis Yamaguti, 1934 infecting the tomato hind Cephalopholis sonnerati (Serranidae) in Saudi Arabia
Jawahir Alghamdi, Saleh Al-Quraishy, Rewaida Abdel-Gaber
Journal of King Saud University - Science.2022; 34(4): 101936. CrossRef
Morphological and molecular analyses of Paropecoelus saudiae sp. nov. (Plagiorchiida: Opecoelidae), a trematoda parasite of Parupeneus rubescens (Mullidae) from the Arabian Gulf
Rewaida Abdel-Gaber, Saleh Al Quraishy, Mohamed A. Dkhil, Maysar Abu Hawsah, Lamia Bakr, Sherein Maher
Journal of King Saud University - Science.2020; 32(3): 2243. CrossRef
Phyllodistomum vaili(Plagiorchiida: Gorgoderidae) infecting Parupeneus rubescens (Perciformes: Mullidae): morphology and phylogeny
Rewaida Abdel-Gaber, Saleh Al Quraishy, Mohamed Abdel Monem Dkhil, Maysar Abu Hawsah, Masheil Alghamdi, Arwa Althomali, Lamia Bakr, Sherein Maher, Almahy El-Mallah
Revista Brasileira de Parasitologia Veterinária.2020;[Epub] CrossRef
Genetic diversity of the potentially therapeutic tapeworm Hymenolepis diminuta (Cestoda: Cyclophyllidea)
Lucie Řežábková, Jan Brabec, Milan Jirků, Marc Dellerba, Roman Kuchta, David Modrý, William Parker, Kateřina Jirků Pomajbíková
Parasitology International.2019; 71: 121. CrossRef
Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China
Xiao Xiao, Rui Qi, Hui-Ju Han, Jian-Wei Liu, Xiang-Rong Qin, Li-Zhu Fang, Chuan-Min Zhou, Xiao-Qing Gong, Si-Cong Lei, Xue-Jie Yu
International Journal for Parasitology: Parasites and Wildlife.2019; 10: 274. CrossRef
A new species of Steringotrema Odhner, 1911 (Trematoda: Fellodistomidae) from the New Zealand sole Peltorhamphus novaezeelandiae Günther off Kaka point in the Catlins, South Island, New Zealand
Gerardo Pérez-Ponce de León, Thibaut Anglade, Haseeb S. Randhawa
Systematic Parasitology.2018; 95(2-3): 213. CrossRef
Schistosomes and Microfilarial Parasites in Magellanic Penguins
R. E. T. Vanstreels, C. H. Gardiner, M. J. Yabsley, L. Swanepoel, C. K. M. Kolesnikovas, R. P. Silva-Filho, A. C. Ewbank, J. L. Catão-Dias
Journal of Parasitology.2018; 104(3): 322. CrossRef
Complete mitochondrial genome of the first deep-sea spongicolid shrimp Spongiocaris panglao (Decapoda: Stenopodidea): Novel gene arrangement and the phylogenetic position and origin of Stenopodidea
Shao'e Sun, Zhongli Sha, Yanrong Wang
Gene.2018; 676: 123. CrossRef
Design and application of a novel two-amplicon approach for defining eukaryotic microbiota
Ana Popovic, Celine Bourdon, Pauline W. Wang, David S. Guttman, Wieger Voskuijl, Michael E. Grigg, Robert H. J. Bandsma, John Parkinson
Microbiome.2018;[Epub] CrossRef
Comparative mitochondrial genomics among Spirometra (Cestoda: Diphyllobothriidae) and the molecular phylogeny of related tapeworms
Xi Zhang, Jiang Yang Duan, Ya Li Shi, Peng Jiang, De Jun Zeng, Zhong Quan Wang, Jing Cui
Molecular Phylogenetics and Evolution.2017; 117: 75. CrossRef
Fishborne zoonotic heterophyid infections: An update
Jong-Yil Chai, Bong-Kwang Jung
Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef
Molecular phylogeny of Cyclophyllidea (Cestoda: Eucestoda): an in-silico analysis based on mtCOI gene
Sunil Sharma, Damanbha Lyngdoh, Bishnupada Roy, Veena Tandon
Parasitology Research.2016; 115(9): 3329. CrossRef
Sparganosis in wild boar ( Sus scrofa ) – Implications for veterinarians, hunters, and consumers
Marta Kołodziej-Sobocińska, Mariusz Miniuk, Iwona Ruczyńska, Małgorzata Tokarska
Veterinary Parasitology.2016; 227: 115. CrossRef
The complete mitochondrial genome of the tapeworm Cladotaenia vulturi (Cestoda: Paruterinidae): gene arrangement and phylogenetic relationships with other cestodes
Aijiang Guo
Parasites & Vectors.2016;[Epub] CrossRef
The complete mitochondrial genome of Anoplocephala perfoliata, the first representative for the family Anoplocephalidae
Aijiang Guo
Parasites & Vectors.2015;[Epub] CrossRef
The first report of sparganosis (Spirometra sp.) in Eurasian badger (Meles meles)
Marta Kołodziej-Sobocińska, Małgorzata Tokarska, Rafał Kowalczyk
Parasitology International.2014; 63(2): 397. CrossRef
Multilocus sequence evaluation for differentiating species of the trematode Family Gastrothylacidae, with a note on the utility of mitochondrial COI motifs in species identification
Sudeep Ghatani, Jollin Andrea Shylla, Bishnupada Roy, Veena Tandon
Gene.2014; 548(2): 277. CrossRef
Description of two new species of the genus Thaparocleidus Jain, 1952 (Monogenea, Dactylogyridae) from freshwater fish in India: morphological and molecular phylogenetic evidence
A. Chaudhary, H.S. Singh
Journal of Helminthology.2013; 87(2): 160. CrossRef
Molecular characterization of the parasitic tapeworm Bertiella studeri from the island of Mauritius
Nawsheen Taleb-Hossenkhan, Suress Bhagwant
Parasitology Research.2012; 110(2): 759. CrossRef
Secondary structure and phylogenetic utility of the ribosomal large subunit (28S) in monogeneans of the genus Thaparocleidus and Bifurcohaptor (Monogenea: Dactylogyridae)
Anshu Chaudhary, Hridaya Shanker Singh
Journal of Parasitic Diseases.2012;[Epub] CrossRef
The life cycle of Asymphylodora perccotti sp. n. (Trematoda: Lissorchiidae) in the Russian Southern Far East
Vladimir V. Besprozvannykh, Alexey V. Ermolenko, Dmitry M. Atopkin
Parasitology International.2012; 61(2): 235. CrossRef
Geographic distribution modelling for ruminant liver flukes (Fasciola hepatica) in south-eastern Europe
Vaia Kantzoura, Marc K. Kouam, Haralambos Feidas, Denitsa Teofanova, Georgios Theodoropoulos
International Journal for Parasitology.2011; 41(7): 747. CrossRef
Genetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini from Savannakhet, Lao PDR by multilocus enzyme electrophoresis
Nadda Kiatsopit, Paiboon Sithithaworn, Jiraporn Sithithaworn, Thidarut Boonmars, Smarn Tesana, Opal Pitaksakulrat, Weerachai Saijuntha, Trevor N. Petney, Ross H. Andrews
Parasitology Research.2011; 108(1): 211. CrossRef