Parasites, Hosts and Diseases

"recombinant antigen"

Original Articles

Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis: Khadijeh Khanaliha, Mohammad Hossein Motazedian, Bahram Kazemi, Bahador Shahriari, Mojgan Bandehpour, Zarin Sharifniya; Korean J Parasitol 2014;52(2):137-142.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.137

Abstract
PDF

Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commercial ELISA. The recombinant plasmids were transformed to JM109 strain of Escherichia coli, and the recombinants were expressed and purified. Recombinant SAG1, SAG2, and SAG3 antigens were evaluated using different groups of sera in an ELISA system, and the results were compared to those of a commercial IgG and IgM ELISA kit. The sensitivity and specificity of recombinant surface antigens for detection of anti-Toxoplasma IgG in comparison with commercially available ELISA were as follows: SAG1 (93.6% and 92.9%), SAG2 (100.0% and 89.4%), and SAG3 (95.4% and 91.2%), respectively. A high degree of agreement (96.9%) was observed between recombinant SAG2 and commercial ELISA in terms of detecting IgG anti-Toxoplasma antibodies. P22 had the best performance in detecting anti-Toxoplasma IgM in comparison with the other 2 recombinant antigens. Recombinant SAG1, SAG2, and SAG3 could all be used for diagnosis of IgG-specific antibodies against T. gondii.

Citations

Citations to this article as recorded by

IgM Antibody Detection as a Diagnostic Marker for Acute Toxoplasmosis: Current Status of Studies and Main Limitations
Karolina Sołowińska, Lucyna Holec-Gąsior
Antibodies.2025; 14(2): 44. CrossRef
Analysis of the Correlation Between Toxoplasma gondii Seropositivity and Alzheimer’s Disease
Jianjun Wang, Ping Lin, Dan Li, Biyu Yang, Jiaqi Wang, Meng Feng, Xunjia Cheng
Pathogens.2024; 13(11): 1021. CrossRef
Seroprevalence and Epidemiology of Toxoplasma gondii in Animals in the Qinghai-Tibetan Plateau Area, China
Guojing Li, Wangli Zheng, Jinfang Yang, Tongsheng Qi, Yongcai He, Wangkai Chen, Hejia Ma, Yali Sun, Ying Li, Ming Kang, Jixu Li
Pathogens.2021; 10(4): 432. CrossRef
Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen
Min-Ju Kim, Jie Mao, Hae-Ji Kang, Ki-Back Chu, Fu-Shi Quan
The Korean Journal of Parasitology.2021; 59(6): 565. CrossRef
Prevalence of Toxoplasma gondii parasite in captive Mexican jaguars determined by recombinant surface antigens (SAG1) and dense granular antigens (GRA1 and GRA7) in ELISA-based serodiagnosis
Alejandro Reynoso-Palomar, Dulce Moreno-Gálvez, Abel Villa-Mancera
Experimental Parasitology.2020; 208: 107791. CrossRef
Evaluation of a PCR assay for diagnosis of toxoplasmosis in serum and peripheral blood mononuclear cell among HIV/AIDS patients
Farah Bokharaei-Salim, Abdoulreza Esteghamati, Khadijeh Khanaliha, Saeed Kalantari, Shirin Sayyahfar, Tahereh Donyavi, Saba Garshasbi, Qasem Asgari, Borna Salemi
Journal of Parasitic Diseases.2020; 44(1): 159. CrossRef
Toxoplasma gondii seropositivity and serointensity and cognitive function in adults
Shawn D. Gale, Lance D. Erickson, Evan L. Thacker, Elizabeth L. Mitchell, Bruce L. Brown, Dawson W. Hedges, Mathieu Nacher
PLOS Neglected Tropical Diseases.2020; 14(10): e0008733. CrossRef
Human toxoplasmosis in Mozambique: gaps in knowledge and research opportunities
Leonardo Manuel, Gabriela Santos-Gomes, Emilia V. Noormahomed
Parasites & Vectors.2020;[Epub] CrossRef
The soluble fraction of Neospora caninum treated with PI-PLC is dominated by NcSRS29B and NcSRS29C
Marcos Alexandre Bezerra, Luiz Miguel Pereira, Luciana Baroni, Ana Patrícia Yatsuda
Experimental Parasitology.2019; 204: 107731. CrossRef
Research Progress of Antigens Related to the Process of Toxoplasma gondii Invading Host Cell
晓敬孙
Open Journal of Natural Science.2019; 07(06): 585. CrossRef
Toxoplasma gondii-positive human sera recognise intracellular tachyzoites and bradyzoites with diverse patterns of immunoreactivity
Marijo S. Roiko, Kaice LaFavers, Diane Leland, Gustavo Arrizabalaga
International Journal for Parasitology.2018; 48(3-4): 225. CrossRef
Development of an immunochromatographic test based on monoclonal antibodies against surface antigen 3 (TgSAG3) for rapid detection of Toxoplasma gondii
Jiaqing Luo, Hongchao Sun, Xianfeng Zhao, Suhua Wang, Xunhui Zhuo, Yi Yang, Xueqiu Chen, Chaoqun Yao, Aifang Du
Veterinary Parasitology.2018; 252: 52. CrossRef
Antigens to Detect the Acute Phase of Toxoplasmosis in Pregnant Women: Standardized Comparison
Juan Gabriel Costa, María Julia Vilariño
Biomarkers in Medicine.2018; 12(5): 517. CrossRef
P35 and P22 Toxoplasma gondii antigens abbreviate regions to diagnose acquired toxoplasmosis during pregnancy: toward single-sample assays
Juan G. Costa, Leandro E. Peretti, Valeria S. García, Luz Peverengo, Verónica D.G. González, Luis M. Gugliotta, Maria L. Dalla Fontana, Claudia M. Lagier, Iván S. Marcipar
Clinical Chemistry and Laboratory Medicine (CCLM).2017;[Epub] CrossRef
Cloning and Sequencing of Truncated Toxoplasma gondii Subtilisin-Like 1 Antigen
Ahmad Rouhizadeh, Ata A Ghadiri, Mohammad Razi Jalali, Masoud Ghorbanpour, Mohammad Hossein Razi Jalali
Zahedan Journal of Research in Medical Sciences.2016;[Epub] CrossRef
Evaluation of Recombinant SRS3 Antigen for Diagnosis of Toxoplasmosis by Enzyme-Linked Immunosorbent Assay
Seyedeh Mahya Marashiyan, Fatemeh Moradian, Geita Saadatnia, Majid Golkar
Archives of Clinical Infectious Diseases.2016;[Epub] CrossRef
Cloning and Expression of Major Surface Antigen 1 Gene of Toxoplasma gondii RH Strain Using the Expression Vector pVAX1 in Chinese Hamster Ovary Cells
Rahman Abdizadeh, Sharif Maraghi, Ata A. Ghadiri, Mehdi Tavalla, Saeedeh Shojaee
Jundishapur Journal of Microbiology.2015;[Epub] CrossRef

11,482 View
109 Download
17 Web of Science
Crossref

A Recombinant Plasmodium vivax Apical Membrane Antigen-1 to Detect Human Infection in Iran: Afsaneh Motevalli Haghi, Mohammad Reza Khoramizade, Mehdi Nateghpour, Mehdi Mohebali, Gholam Hossein Edrissian, Mohammad Reza Eshraghian, Zargham Sepehrizadeh; Korean J Parasitol 2012;50(1):15-21.
Published online March 6, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.1.15

Abstract
PDF

In Iran, Plasmodium vivax is responsible for more than 80% of the infected cases of malaria per year. Control interventions for vivax malaria in humans rely mainly on developed diagnostic methods. Recombinant P. vivax apical membrane antigen-1 (rPvAMA-1) has been reported to achieve designing rapid, sensitive, and specific molecular diagnosis. This study aimed to perform isolation and expression of a rPvAMA-1, derived from Iranian patients residing in an endemic area. Then, the diagnostic efficiency of the characterized Iranian PvAMA-1 was assessed using an indirect ELISA method. For this purpose, a partial region of AMA-1 gene was amplified, cloned, and expressed in pET32a plasmid. The recombinant His-tagged protein was purified and used to coat the ELISA plate. Antibody detection was assessed by indirect ELISA using rPvAMA-1. The validity of the ELISA method for detection of anti-P. vivax antibodies in the field was compared to light microscopy on 84 confirmed P. vivax patients and compared to 84 non-P. vivax infected individuals. The ELISA cut-off value was calculated as the mean+2SD of OD values of the people living in malaria endemic areas from a south part of Iran. We found a cut-off point of OD=0.311 that showed the best correlation between the sera confirmed with P. vivax infection and healthy control sera. A sensitivity of 81.0% and specificity of 84.5% were found at this cut off titer. A good degree of statistical agreement was found between ELISA using rPvAMA-1 and light microscopy (0.827) by Kappa analysis.

Citations

Citations to this article as recorded by

Immunogenic and diagnostic potential of recombinant apical membrane antigen-1 from Plasmodium malariae
Moyan Li, Tingting Liu, Yuerong Wang, Luwen Zhang, Fanbo Lu, Jinxing Xia, Meijuan Zheng, Min Zhang, Bo Wang, Yuanhong Xu
Diagnostic Microbiology and Infectious Disease.2024; 110(3): 116480. CrossRef
A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery
Seda Yerlikaya, Ewurama D A Owusu, Augustina Frimpong, Robert Kirk DeLisle, Xavier C Ding
Clinical Infectious Diseases.2022; 74(1): 40. CrossRef
Structure-genetic diversity and recombinant protein of circumsporozoite protein (CSP) of vivax malaria antigen: A potential malaria vaccine candidate
Vahid Raissi, Soudabeh Etemadi, Muhammad Ibrahim Getso, Ahmad Mehravaran, Omid Raiesi
Gene Reports.2021; 23: 101132. CrossRef
Serological responses to a soluble recombinant circumsporozoite protein-VK210 of Plasmodium vivax (rPvCSP-VK210) among Iranian malaria patients
Mehdi Nateghpour, Soudabeh Etemadi, Afsaneh Motevalli Haghi, Hamid Eslami, Mehdi Mohebali, Leila Farivar
European Journal of Medical Research.2021;[Epub] CrossRef
The immunology of Plasmodium vivax malaria
Lis R. Antonelli, Caroline Junqueira, Joseph M Vinetz, Douglas T. Golenbock, Marcelo U. Ferreira, Ricardo T. Gazzinelli
Immunological Reviews.2020; 293(1): 163. CrossRef
Blood-stage Plasmodium vivax antibody dynamics in a low transmission setting: A nine year follow-up study in the Amazon region
Camilla V. Pires, Jessica R. S. Alves, Barbara A. S. Lima, Ruth B. Paula, Helena L. Costa, Leticia M. Torres, Taís N. Sousa, Irene S. Soares, Bruno A. M. Sanchez, Cor J. F. Fontes, Francis B. Ntumngia, John H. Adams, Flora S. Kano, Luzia H. Carvalho, Gerh
PLOS ONE.2018; 13(11): e0207244. CrossRef
What Is Known about the Immune Response Induced by Plasmodium vivax Malaria Vaccine Candidates?
Carolina López, Yoelis Yepes-Pérez, Natalia Hincapié-Escobar, Diana Díaz-Arévalo, Manuel A. Patarroyo
Frontiers in Immunology.2017;[Epub] CrossRef
Detection of human malaria using recombinant Plasmodium knowlesi merozoire surface protein-1 (MSP-119) expressed in Escherichia coli
Parthasarathy Sonaimuthu, Fei Wen Cheong, Lit Chein Chin, Rohela Mahmud, Mun Yik Fong, Yee Ling Lau
Experimental Parasitology.2015; 153: 118. CrossRef
Immunogenicity of bacterial-expressed recombinant Plasmodium knowlesi merozoite surface protein-142 (MSP-142)
Fei Wen Cheong, Mun Yik Fong, Yee Ling Lau, Rohela Mahmud
Malaria Journal.2013;[Epub] CrossRef
Evaluation of Recombinant Plasmodium knowlesi Merozoite Surface Protein-133 for Detection of Human Malaria
Yee Ling Lau, Fei Wen Cheong, Rohela Mahmud, Mun Yik Fong
The American Journal of Tropical Medicine and Hygiene.2013; 88(5): 835. CrossRef

8,383 View
81 Download
Crossref

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection: Jung-Hwa Cho, Woo-Suk Chung, Kyoung-Ju Song, Byoung-Kuk Na, Seung-Won Kang, Chul-Yong Song, Tong-Soo Kim; Korean J Parasitol 2005;43(1):19-25.
Published online March 20, 2005; DOI: https://doi.org/10.3347/kjp.2005.43.1.19

Abstract
PDF

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and two dense granule proteins (NcDG1 and NcDG2) of N. caninum tachyzoites were expressed in E. coli, respectively. An in vitro neutralization assay using polyclonal antisera raised against each recombinant antigen showed inhibitory effects on the invasion of N. caninum tachyzoites into host cells. Separate groups of gerbils were immunized with the purified recombinant proteins singly or in combinations and animals were then challenged with N.caninum. Following these experimental challenges, the protective efficacy of each vaccination was determined by assessing animal survival rate. All experimental groups showed protective effects of different degrees against experimental infection. The highest protection efficacy was observed for combined vaccination with NcSRS2 and NcDG1. Our results indicate that combined vaccination with the N. caninum recombinant antigens, NcSRS2 and NcDG1, induces the highest protective effect against N. caninum infection in vitro and in vivo.

Citations

Citations to this article as recorded by

Molecular characterization of Neospora caninum major antigens NcSAG1 and NcSRS2
Soledad Echeverría, Federico Carrión, Martín Soñora, Andrés Cabrera, Carlos Robello
Royal Society Open Science.2025;[Epub] CrossRef
A Listeria monocytogenes-Based Vaccine Formulation Reduces Vertical Transmission and Leads to Enhanced Pup Survival in a Pregnant Neosporosis Mouse Model
Dennis Imhof, William Robert Pownall, Camille Monney, Anna Oevermann, Andrew Hemphill
Vaccines.2021; 9(12): 1400. CrossRef
Interferon-γ-dependent protection against Neospora caninum infection conferred by mucosal immunization in IL-12/IL-23 p40-deficient mice
Pedro Ferreirinha, Ricardo Fróis-Martins, Luzia Teixeira, António Rocha, Manuel Vilanova, Alexandra Correia
Vaccine.2018; 36(32): 4890. CrossRef
On the application of reverse vaccinology to parasitic diseases: a perspective on feature selection and ranking of vaccine candidates
Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis
International Journal for Parasitology.2017; 47(12): 779. CrossRef
Mucosal immunization confers long-term protection against intragastrically established Neospora caninum infection
Pedro Ferreirinha, Alexandra Correia, Maria Teixeira-Coelho, Hugo Osório, Luzia Teixeira, António Rocha, Manuel Vilanova
Vaccine.2016; 34(50): 6250. CrossRef
A 78 kDa host cell invasion protein of Neospora caninum as a potential vaccine candidate
Qiang Lv, Shenyang Xing, Pengtao Gong, Le Chang, Zhengzheng Bian, Lidong Wang, Xichen Zhang, Jianhua Li
Experimental Parasitology.2015; 148: 56. CrossRef
Discovering a vaccine against neosporosis using computers: is it feasible?
Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis
Trends in Parasitology.2014; 30(8): 401. CrossRef
Cloning and expression of Neospora caninum dense-granule 7 in E. coli
Marziye Kefayat, Hossein Hamidinejat, Masoud Reza Seifiabadshapoori, Mohammad Mehdi Namavari, Parviz Shayan, Saad Gooraninejad
Journal of Parasitic Diseases.2014; 38(2): 196. CrossRef
Vaccines against neosporosis: What can we learn from the past studies?
Thierry Monney, Andrew Hemphill
Experimental Parasitology.2014; 140: 52. CrossRef
A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
Yanina P. Hecker, Verónica Cóceres, Silvina E. Wilkowsky, José M. Jaramillo Ortiz, Eleonora L. Morrell, Andrea E. Verna, Agustina Ganuza, Dora B. Cano, Lilian Lischinsky, Sergio O. Ángel, Patricia Zamorano, Anselmo C. Odeón, María R. Leunda, Carlos M. Cam
Veterinary Immunology and Immunopathology.2014; 162(3-4): 142. CrossRef
A guide to in silico vaccine discovery for eukaryotic pathogens
S. J. Goodswen, P. J. Kennedy, J. T. Ellis
Briefings in Bioinformatics.2013; 14(6): 753. CrossRef
ELISAs based on rNcGRA7 and rNcSAG1 antigens as an indicator of Neospora caninum activation
Jun Hiasa, Junko Kohara, Maki Nishimura, Xuenan Xuan, Hiromi Tokimitsu, Yoshifumi Nishikawa
Veterinary Parasitology.2012; 187(3-4): 379. CrossRef
Vaccines against a Major Cause of Abortion in Cattle, Neospora caninum Infection
Thierry Monney, Karim Debache, Andrew Hemphill
Animals.2011; 1(3): 306. CrossRef
Comparative efficacy of immunization with inactivated whole tachyzoites versus a tachyzoite-bradyzoite mixture against neosporosis in mice
S. ROJO-MONTEJO, E. COLLANTES-FERNÁNDEZ, S. RODRÍGUEZ-MARCOS, F. PÉREZ-ZABALLOS, I. LÓPEZ-PÉREZ, A. PRENAFETA, L. M. ORTEGA-MORA
Parasitology.2011; 138(11): 1372. CrossRef
Transgenic Neospora caninum strains constitutively expressing the bradyzoite NcSAG4 protein proved to be safe and conferred significant levels of protection against vertical transmission when used as live vaccines in mice
V. Marugán-Hernández, L.M. Ortega-Mora, A. Aguado-Martínez, E. Jiménez-Ruíz, G. Álvarez-García
Vaccine.2011; 29(44): 7867. CrossRef
Selection of Neospora caninum antigens stimulating bovine CD4+ve T cell responses through immuno-potency screening and proteomic approaches
Mara S Rocchi, Paul M Bartley, Neil F Inglis, Esther Collantes-Fernandez, Gary Entrican, Frank Katzer, Elisabeth A Innes
Veterinary Research.2011; 42(1): 91. CrossRef
RecNcMIC3-1-R is a microneme- and rhoptry-based chimeric antigen that protects against acute neosporosis and limits cerebral parasite load in the mouse model for Neospora caninum infection
Thierry Monney, David Rütti, Michelle Schorer, Karim Debache, Denis Grandgirard, Stephen L. Leib, Andrew Hemphill
Vaccine.2011; 29(40): 6967. CrossRef
Immunization of female BALB/c mice with Neospora cyclophilin and/or NcSRS2 elicits specific antibody response and prevents against challenge infection by Neospora caninum
Wenbin Tuo, Yan Zhao, Daming Zhu, Mark C. Jenkins
Vaccine.2011; 29(13): 2392. CrossRef
Intraperitoneal and intra-nasal vaccination of mice with three distinct recombinantNeospora caninumantigens results in differential effects with regard to protection against experimental challenge withNeospora caninumtachyzoites
K. DEBACHE, C. GUIONAUD, F. ALAEDDINE, A. HEMPHILL
Parasitology.2010; 137(2): 229. CrossRef
Characterisation of NcGRA7 and NcSAG4 proteins: Immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites
Adriana Aguado-Mart?nez, Gema ?lvarez-Garc?a, Gereon Schares, Ver?nica Risco-Castillo, Aurora Fern?ndez-Garc?a, Virginia Marug?n-Hern?ndez, Luis Ortega-Mora
Acta Parasitologica.2010;[Epub] CrossRef
CpG-ODN combined with Neospora caninum lysate, but not with excreted-secreted antigen, enhances protection against infection in mice
Dâmaso P. Ribeiro, Marina M.P. Freitas, Mariana R.D. Cardoso, Ana C.A.M. Pajuaba, Neide M. Silva, Tiago W.P. Mineo, João S. Silva, José R. Mineo, Deise A.O. Silva
Vaccine.2009; 27(19): 2570. CrossRef
Characterization of tissue distribution and histopathological lesions in Neospora caninum experimentally infected gerbils
Seung-Won Kang, Sung-Soo Park, Se-Eun Choe, Young-Hwa Jean, Suk-Chan Jung, Keun Kim, Dong Van Quyen
Parasitology Research.2009; 104(6): 1261. CrossRef
Failure of a vaccine using immunogenic recombinant proteins rNcSAG4 and rNcGRA7 against neosporosis in mice
Adriana Aguado-Martínez, Gema Álvarez-García, Aurora Fernández-García, Verónica Risco-Castillo, Virginia Marugán-Hernández, Luis M. Ortega-Mora
Vaccine.2009; 27(52): 7331. CrossRef
Vaccination with recombinant NcROP2 combined with recombinant NcMIC1 and NcMIC3 reduces cerebral infection and vertical transmission in mice experimentally infected with Neospora caninum tachyzoites
Karim Debache, Ferial Alaeddine, Christophe Guionaud, Thierry Monney, Joachim Müller, Maria Strohbusch, Stephen L. Leib, Denis Grandgirard, Andrew Hemphill
International Journal for Parasitology.2009; 39(12): 1373. CrossRef
Neospora caninum – How close are we to development of an efficacious vaccine that prevents abortion in cattle?
Michael P. Reichel, John T. Ellis
International Journal for Parasitology.2009; 39(11): 1173. CrossRef
Immunization with Oligomannose-Coated Liposome-Entrapped Dense Granule Protein 7 Protects Dams and Offspring fromNeospora caninumInfection in Mice
Yoshifumi Nishikawa, Houshuang Zhang, Yuzuru Ikehara, Naoya Kojima, Xuenan Xuan, Naoaki Yokoyama
Clinical and Vaccine Immunology.2009; 16(6): 792. CrossRef
Vaccination of mice with recombinant NcROP2 antigen reduces mortality and cerebral infection in mice infected with Neospora caninum tachyzoites
Karim Debache, Christophe Guionaud, Ferial Alaeddine, Meike Mevissen, Andrew Hemphill
International Journal for Parasitology.2008; 38(12): 1455. CrossRef
Prevention of lethal experimental infection of C57BL/6 mice by vaccination with Brucella abortus strain RB51 expressing Neospora caninum antigens
Sheela Ramamoorthy, Neelima Sanakkayala, Ramesh Vemulapalli, Robert B. Duncan, David S. Lindsay, Gerhart S. Schurig, Stephen M. Boyle, Ramanathan Kasimanickam, Nammalwar Sriranganathan
International Journal for Parasitology.2007; 37(13): 1521. CrossRef
Reduced cerebral infection of Neospora caninum in BALB/c mice vaccinated with recombinant Brucella abortus RB51 strains expressing N. caninum SRS2 and GRA7 proteins
Ramesh Vemulapalli, Neelima Sanakkayala, Jatinder Gulani, Gerhardt G. Schurig, Stephen M. Boyle, David S. Lindsay, Nammalwar Sriranganathan
Veterinary Parasitology.2007; 148(3-4): 219. CrossRef
VACCINATION WITH MICRONEME PROTEIN NCMIC4 INCREASES MORTALITY IN MICE INOCULATED WITH NEOSPORA CANINUM
Sangeetha Srinivasan, Joachim Mueller, Angela Suana, Andrew Hemphill
Journal of Parasitology.2007; 93(5): 1046. CrossRef
Protecting babies: vaccine strategies to prevent foetopathy inNeospora caninum‐infected cattle
D. J. L. WILLIAMS, A. J. TREES
Parasite Immunology.2006; 28(3): 61. CrossRef
Vaccination as a control strategy against the coccidial parasitesEimeria,ToxoplasmaandNeospora
E. A. INNES, A. N. VERMEULEN
Parasitology.2006; 133(S2): S145. CrossRef
Immunization with native surface protein NcSRS2 induces a Th2 immune response and reduces congenital Neospora caninum transmission in mice
G.J. Haldorson, B.A. Mathison, K. Wenberg, P.A. Conrad, J.P. Dubey, A.J. Trees, I. Yamane, T.V. Baszler
International Journal for Parasitology.2005; 35(13): 1407. CrossRef

8,948 View
94 Download
Crossref

ELISA detection of vivax malaria with recombinant multiple stage-specific antigens and its application to survey of residents in endemic areas: Sera Kim, Hye-Jin Ahn, Tong-Soo Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(4):203-207.
Published online December 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.4.203

Abstract
PDF

An ELISA was developed for the diagnosis of vivax malaria using multiple stage-specific recombinant antigens of Plasmodium vivax. The DNA from the whole blood of a malaria patient was used as template to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), merozoite surface protein (MSP-1), apical merozoite antigen (AMA-1), serine repeat antigen (SERA), and exported antigen (EXP-1). Each amplified DNA fragment was inserted into pQE30 plasmid to induce the expression of His-tagged protein in Escherichia coli (M15 strain) by IPTG. His-tagged proteins were purified by Ni-NTA metal-affinity chromatography and used as antigens for ELISA with patient sera that were confirmed previously by blood smear examinations. When applied to patient sera, 122 (80.3%) out of 152 vivax malaria cases reacted to at least one antigen, while no reactions were observed with 128 uninfected serum samples. We applied this ELISA to the screening of 3,262 civilian residents in endemic regions near the DMZ, which resulted in 236 positively detected (7.2%) cases. This method can be applied to serological diagnosis and mass screening in endemic regions, or can be used as a safety test for transfusion blood in endemic areas.

Citations

Citations to this article as recorded by

Using Serological Markers for the Surveillance of Plasmodium vivax Malaria: A Scoping Review
Lejla Kartal, Ivo Mueller, Rhea J. Longley
Pathogens.2023; 12(6): 791. CrossRef
Antibodies Against the Plasmodium vivax Apical Membrane Antigen 1 From the Belem Strain Share Common Epitopes Among Other Worldwide Variants
Ana Caroline Barbosa França, Kátia Sanches Françoso, Rodolfo Ferreira Marques, Gustavo H. G. Trossini, Renan A. Gomes, Marinete M. Póvoa, Maristela G. Cunha, Eduardo L. V. Silveira, Irene S. Soares
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Analyses of the expression, immunohistochemical properties and serodiagnostic potential of Schistosoma japonicum peroxiredoxin-4
Minh-Anh Dang-Trinh, Jose Ma. M. Angeles, Kharleezelle J. Moendeg, Adrian Miki C. Macalanda, Thu-Thuy Nguyen, Luna Higuchi, Shotaro Nakagun, Masashi Kirinoki, Yuichi Chigusa, Yasuyuki Goto, Shin-ichiro Kawazu
Parasites & Vectors.2020;[Epub] CrossRef
Development and optimization of cocktail-ELISA for a unified surveillance of zoonotic schistosomiasis in multiple host species
Kharleezelle J. Moendeg, Jose Ma. M. Angeles, Yasuyuki Goto, Lydia R. Leonardo, Masashi Kirinoki, Elena A. Villacorte, Pilarita T. Rivera, Noboru Inoue, Yuichi Chigusa, Shin-ichiro Kawazu
Parasitology Research.2015; 114(3): 1225. CrossRef
Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients
Ho-Woo Nam, Kyoung Ju Song, Hye Jin Ahn, Zhaoshou Yang, Chom-Kyu Chong, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim
The Korean Journal of Parasitology.2014; 52(2): 143. CrossRef
Immunological markers of Plasmodium vivaxexposure and immunity: a systematic review and meta-analysis
Julia C Cutts, Rosanna Powell, Paul A Agius, James G Beeson, Julie A Simpson, Freya J I Fowkes
BMC Medicine.2014;[Epub] CrossRef
A Recombinant Plasmodium vivax Apical Membrane Antigen-1 to Detect Human Infection in Iran
Afsaneh Motevalli Haghi, Mohammad Reza Khoramizade, Mehdi Nateghpour, Mehdi Mohebali, Gholam Hossein Edrissian, Mohammad Reza Eshraghian, Zargham Sepehrizadeh
The Korean Journal of Parasitology.2012; 50(1): 15. CrossRef
Isolation and Characterization of the MSP1 Genes from Plasmodium malariae and Plasmodium ovale
George J. Dawson, Suresh M. Desai, Larry Birkenmeyer, A. Scott Muerhoff
The American Journal of Tropical Medicine and Hygiene.2010; 82(6): 996. CrossRef
Detection ofPlasmodium falciparum,P. vivax,P. ovale, andP. malariaeMerozoite Surface Protein 1-p19 Antibodies in Human Malaria Patients and Experimentally Infected Nonhuman Primates
A. Scott Muerhoff, Larry G. Birkenmeyer, Ruthie Coffey, Bruce J. Dille, John W. Barnwell, William E. Collins, Joann S. Sullivan, George J. Dawson, Suresh M. Desai
Clinical and Vaccine Immunology.2010; 17(10): 1631. CrossRef
A new ELISA kit which uses a combination of Plasmodium falciparum extract and recombinant Plasmodium vivax antigens as an alternative to IFAT for detection of malaria antibodies
Cecile Doderer, Aurelie Heschung, Phillippe Guntz, Jean-Pierre Cazenave, Yves Hansmann, Alexandre Senegas, Alexander W Pfaff, Tamer Abdelrahman, Ermanno Candolfi
Malaria Journal.2007;[Epub] CrossRef

8,003 View
81 Download
Crossref

Brief Communication

ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera: Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(3):175-177.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.175

Abstract
PDF

An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a 6 × His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals.

Citations

Citations to this article as recorded by

Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Roloff, Fábio Pereira Leivas Leite
Revista Brasileira de Parasitologia Veterinária.2015; 24(2): 148. CrossRef
Diagnostic Potential of Anti-rNcp-43 Polyclonal Antibodies for the Detection of Neospora caninum
Gizele Lima de Sá, Diene de Borba Pacheco, Leonardo Garcia Monte, Francine Alves Sinnott, Marina Amaral Xavier, Caroline Rizzi, Sibele Borsuk, Maria Elisabeth Aires Berne, Renato Andreotti, Cláudia Pinho Hartleben
Current Microbiology.2014; 68(4): 472. CrossRef
Expression ofNeospora caninumNcSRS2 surface protein inPichia pastorisand its application for serodiagnosis ofNeosporainfection
Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Rollof, Fábio Pereira Leivas Leite
Pathogens and Global Health.2013; 107(3): 116. CrossRef
Development of an indirect ELISA-NcSRS2 for detection of Neospora caninum antibodies in cattle
Sibele Borsuk, Renato Andreotti, Fábio Pereira Leivas Leite, Luciano da Silva Pinto, Simone Simionatto, Claudia Pinho Hartleben, Marcelo Goetze, Leandra Marla Oshiro, Maria de Fátima Cepa Matos, Maria Elisabeth Aires Berne
Veterinary Parasitology.2011; 177(1-2): 33. CrossRef
Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA
Jing Liu, Jinshu Yu, Ming Wang, Qun Liu, Wei Zhang, Chong Deng, Jun Ding
Veterinary Parasitology.2007; 143(3-4): 358. CrossRef
Epidemiology and Control of Neosporosis andNeospora caninum
J. P. Dubey, G. Schares, L. M. Ortega-Mora
Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef
Diagnosis of bovine neosporosis: Recent advances and perspectives
Luis Ortega-Mora, Aurora Fernández-García, Mercedes Gómez-Bautista
Acta Parasitologica.2006; 51(1): 1. CrossRef
Diagnosis of bovine neosporosis
J.P. Dubey, G. Schares
Veterinary Parasitology.2006; 140(1-2): 1. CrossRef
Comparison of proteome and antigenic proteome between two Neospora caninum isolates
Yong-Seung Shin, Gee-Wook Shin, Young-Rim Kim, Eun-Young Lee, Hyang-Hee Yang, K.J. Palaksha, Hee-Jeong Youn, Jae-Hoon Kim, Dae-Yong Kim, A.E. Marsh, J. Lakritz, Tae-Sung Jung
Veterinary Parasitology.2005; 134(1-2): 41. CrossRef
HPLC purification of recombinant NcGRA6 antigen improves enzyme-linked immunosorbent assay for serodiagnosis of bovine neosporosis
M.C. Jenkins, R. Fetterer, G. Schares, C. Björkman, W. Wapenaar, M. McAllister, J.P. Dubey
Veterinary Parasitology.2005; 131(3-4): 227. CrossRef
Identification of antigenic proteins from Neospora caninum recognized by bovine immunoglobulins M, E, A and G using immunoproteomics
Yong‐seung Shin, Eung‐goo Lee, Gee‐wook Shin, Young‐rim Kim, Eun‐young Lee, Jae‐hoon Kim, Hwan Jang, Laurel J. Gershwin, Dae‐yong Kim, Yong‐hwan Kim, Gon‐sup Kim, Myung‐deuk Suh, Tae‐sung Jung
PROTEOMICS.2004; 4(11): 3600. CrossRef
Development of competitive ELISA for neosporosis by employing immunoproteomics
Yong-seung Shin, Eung-goo Lee, Gee-wook Shin, Young-rim Kim, Eun-young Lee, Jae-hoon Kim, Hwan Jang, Dae-yong Kim, Yong-hwan Kim, Gon-sup Kim, Myung-deuk Suh, Tae-sung Jung
Clinical Proteomics.2004;[Epub] CrossRef

7,942 View
77 Download
Crossref

Original Articles

Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite: Eui-Sun Son, Tong Soo Kim, Ho-Woo Nam; Korean J Parasitol 2001;39(2):171-176.
Published online June 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.2.171

Abstract
PDF

Western blot analysis was performed to diagnose vivax malaria using stage-specific recombinant antigens. Genomic DNA from the whole blood of a malaria patient was used as templates to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), merozoite surface protein (MSP-1), apical merozoite antigen (AMA-1), serine repeat antigen (SERA), and exported antigen (EXP-1) of Plasmodium vivax. Each amplified DNA fragment was inserted into a pGEX-4T plasmid to induce the expression of GST fusion protein in Escherichia coli by IPTG. The bacterial cell extracts were separated on 10% SDS-PAGE followed by western blot analysis with patient sera which was confirmed by blood smear examination. When applied with patient sera, 147 (91.9%) out of 160 vivax malaria, 12 (92.3%) out of 13 falciparum malaria, and all 9 vivax/falciparum mixed malaria reacted with at least one antigen, while no reactions occurred with 20 normal uninfected sera. In the case of vivax malaria, CSP-1 reacted with 128 (80.0%) sera, MSP-1 with 102 (63.8%), AMA-1 with 128 (80.0%), SERA with 115 (71.9%), and EXP-1 with 89 (55.6%), respectively. We obtained higher detection rates when using 5 antigens (91.9%) rather than using each antigen solely (55.6-80%), a combination of 2 (76.3-87.5%), 3 (85.6-90.6%), or 4 antigens (89.4-91.3%). This method can be applied to serological diagnosis, mass screening in endemic regions, or safety test in transfusion of prevalent vivax malaria.

Citations

Citations to this article as recorded by

A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery
Seda Yerlikaya, Ewurama D A Owusu, Augustina Frimpong, Robert Kirk DeLisle, Xavier C Ding
Clinical Infectious Diseases.2022; 74(1): 40. CrossRef
Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients
Iris Aparici-Herraiz, Melisa Gualdrón-López, Carlos J. Castro-Cavadía, Jaime Carmona-Fonseca, María Fernanda Yasnot, Carmen Fernandez-Becerra, Hernando A. del Portillo
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Development of an Immunosensor for PfHRP 2 as a Biomarker for Malaria Detection
Aver Hemben, Jon Ashley, Ibtisam Tothill
Biosensors.2017; 7(3): 28. CrossRef
Expression and Evaluation of Recombinant Plasmodium knowlesi Merozoite Surface Protein-3 (MSP-3) for Detection of Human Malaria
Jeremy Ryan De Silva, Yee-Ling Lau, Mun-Yik Fong, Luzia Helena Carvalho
PLOS ONE.2016; 11(7): e0158998. CrossRef
Evaluation of Recombinant Plasmodium knowlesi Merozoite Surface Protein-133 for Detection of Human Malaria
Yee Ling Lau, Fei Wen Cheong, Rohela Mahmud, Mun Yik Fong
The American Journal of Tropical Medicine and Hygiene.2013; 88(5): 835. CrossRef
Identification of Plasmodium vivax Proteins with Potential Role in Invasion Using Sequence Redundancy Reduction and Profile Hidden Markov Models
Daniel Restrepo-Montoya, David Becerra, Juan G. Carvajal-Patiño, Alvaro Mongui, Luis F. Niño, Manuel E. Patarroyo, Manuel A. Patarroyo, Leonardo Mariño-Ramírez
PLoS ONE.2011; 6(10): e25189. CrossRef
ELISA detection of vivax malaria with recombinant multiple stage-specific antigens and its application to survey of residents in endemic areas
Sera Kim, Hye-Jin Ahn, Tong-Soo Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2003; 41(4): 203. CrossRef

8,538 View
95 Download
Crossref

Immunodiagnosis of clonorchiasis using a recombinant antigen: Tai-Soon Yong, Hye-Jin Yang, Soon-Jung Park, Yu-Kyoung Kim, Du-Ho Lee, Sang-Mi Lee; Korean J Parasitol 1998;36(3):183-190.
Published online September 30, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.3.183

Abstract
PDF

A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a β-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis.

Citations

Citations to this article as recorded by

A point-of-care testing assay for clonorchiasis using a EuNPs-CsTR1 fluorescent probe-based immunoassay
Xiaoxiao Ma, Huiyuan Zhang, Yiming Fang, Jing Wang, Xingyang Wang, Chen Li, Xiaolei Liu, Mingyuan Liu, Bin Tang, Yi Liu, Javier Sotillo
PLOS Neglected Tropical Diseases.2024; 18(4): e0012107. CrossRef
Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
Pyo Yun Cho, Ji-Yun Lee, Tae Im Kim, Jin-Ho Song, Sung-Jong Hong, Won Gi Yoo, Takafumi Tsuboi, Kwon-Soo Ha, Jae-Wan Jung, Satoru Takeo, Eun-Taek Han, Banchob Sripa, Sung-Tae Hong, Jong-Yil Chai, Ho-Woo Nam, Jhang Ho Pak, Tong-Soo Kim, Krystyna Cwiklinski
PLOS Neglected Tropical Diseases.2020; 14(12): e0008998. CrossRef
Cs1, a Clonorchis sinensis-derived serodiagnostic antigen containing tandem repeats and a signal peptide
Na Cheng, Xue-Nian Xu, Yan Zhou, Yu-Ting Dong, Yi-Fang Bao, Bin Xu, Wei Hu, Zheng Feng, Maria Elena Bottazzi
PLOS Neglected Tropical Diseases.2018; 12(8): e0006683. CrossRef
Development of a polymerase chain reaction applicable to rapid and sensitive detection ofClonorchis sinensiseggs in human stool samples
Pyo Yun Cho, Byoung-Kuk Na, Kyung Mi Choi, Jin Su Kim, Shin-Hyeong Cho, Won-Ja Lee, Sung-Bin Lim, Seok Ho Cha, Yun-Kyu Park, Jhang Ho Pak, Hyeong-Woo Lee, Sung-Jong Hong, Tong-Soo Kim
Pathogens and Global Health.2013; 107(5): 253. CrossRef
Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis
Shunyu Li, Jung Guk Shin, Pyo Yun Cho, Tae Im Kim, Sung-Tae Hong, Sung-Jong Hong
Parasitology Research.2011; 108(5): 1295. CrossRef
Proteomics of foodborne trematodes
Rafael Toledo, M. Dolores Bernal, Antonio Marcilla
Journal of Proteomics.2011; 74(9): 1485. CrossRef
Recent Advances in Serodiagnosis for Clonorchiasis
Tae Im Kim, Sung-Jong Hong
Hanyang Medical Reviews.2010; 30(3): 232. CrossRef
Functional Genes and Proteins of Clonorchis sinensis
Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
The Korean Journal of Parasitology.2009; 47(Suppl): S59. CrossRef
Molecular cloning and phylogenetic analysis of Clonorchis sinensis elongation factor-1α
Tae Yun Kim, Pyo Yun Cho, Jong Won Na, Sung-Jong Hong
Parasitology Research.2007; 101(6): 1557. CrossRef
Clonorchis sinensis: Development and evaluation of a nested polymerase chain reaction (PCR) assay
Ammini Parvathi, H. Sanath Kumar, B. Kenchanna Prakasha, Jieyuan Lu, Xuenian Xu, Wei Hu, Zheng Feng, Indrani Karunasagar, Iddya Karunasagar
Experimental Parasitology.2007; 115(3): 291. CrossRef
Clonorchiasis: an update
H.-J. Rim
Journal of Helminthology.2005; 79(3): 269. CrossRef
Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA
Min-Ho Choi, Il Chan Park, Shunyu Li, Sung-Tae Hong
The Korean Journal of Parasitology.2003; 41(1): 35. CrossRef
Immunology and molecular biology of Opisthorchis viverrini infection
S. Wongratanacheewin, R.W. Sermswan, S. Sirisinha
Acta Tropica.2003; 88(3): 195. CrossRef
Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis
Young-Bae Chung, Mejeong Lee, Hyun-Jong Yang, Byung-Suk Chung, Shun-Yu Lee, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2002; 40(2): 83. CrossRef
Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis
Tae Yun Kim, Shin-Yong Kang, Il-Young Ahn, Seung-Yull Cho, Sung-Jong Hong
The Korean Journal of Parasitology.2001; 39(1): 57. CrossRef
Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection
Sung-Jong Hong, Tae Yun Kim, Kye-Yong Song, Woon-Mok Sohn, Shin-Yong Kang
The Korean Journal of Parasitology.2001; 39(4): 307. CrossRef
Cystatin Capture Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Clonorchiasis and Profile of Captured Antigenic Protein ofClonorchis sinensis
Tae Yun Kim, Shin-Yong Kang, Sun Hyo Park, Kom Sukontason, Kabkaew Sukontason, Sung-Jong Hong
Clinical Diagnostic Laboratory Immunology.2001; 8(6): 1076. CrossRef
Identification of a Clonorchis sinensis gene encoding a vitellaria antigenic protein containing repetitive sequences
Hye-Jin Yang, Soon-Jung Park, Kyung-il Im, Tai-Soon Yong
Molecular and Biochemical Parasitology.2000; 111(1): 213. CrossRef