Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and is endemic in many Latin American countries. Diagnosis is based on serologic testing and the WHO recommends two or more serological tests for confirmation. Acidic ribosomal P protein of T. cruzi showed strong reactivity against positive sera of patients, and we cloned the protein after fragmenting it to enhance its antigenicity and solubility. Twelve positive sera of Chagas disease patients were reacted with the fragmented ribosomal P protein using western blot. Detection rate and density for each fragment were determined. Fragments F1R1, F1R2, and F2R1 showed 100% rate of detection, and average density scoring of 2.00, 1.67, and 2.42 from a maximum of 3.0, respectively. Therefore, the F2R1 fragment of the ribosomal P protein of T. cruzi could be a promising antigen to use in the diagnosis of Chagas disease in endemic regions with high specificity and sensitivity.
Citations
Citations to this article as recorded by
In vitro diagnostic methods of Chagas disease in the clinical laboratory: a scoping review Luis C. Ascanio, Savannah Carroll, Alberto Paniz-Mondolfi, Juan David Ramírez Frontiers in Microbiology.2024;[Epub] CrossRef
A refined genome phage display methodology delineates the human antibody response in patients with Chagas disease André Azevedo Reis Teixeira, Luis Rodriguez Carnero, Andréia Kuramoto, Fenny Hui Fen Tang, Carlos Hernique Gomes, Natalia Bueno Pereira, Léa Campos de Oliveira, Regina Garrini, Jhonatas Sirino Monteiro, João Carlos Setubal, Ester Cerdeira Sabino, Renata P iScience.2021; 24(6): 102540. CrossRef
Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy. The protein was not released into the parasitophorous vacuole during or after invasion. The cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg621. The full length cDNA sequence was completed with 5'-RACE as 1,592 bp, which contained open reading frame of 942 bp. The deduced amino acid sequence of Tg621 consisted of a polypeptide of 313 amino acids, with significant homology to ribosomal P proteins (RPP) of other organisms especially high to those of apicomplexan species. The expressed and purified TgRPP was assayed in western blot with the sera of toxoplasmosis patients and normal sera, which resulted in the 74.0% of positive reactions in toxoplasmosis patients whereas 8.3% in normal group. Therefore, the antibody formation against TgRPP in toxoplasmosis patients was regarded as specific for T. gondii infection and suggested a potential autoantibody.
Citations
Citations to this article as recorded by
Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli M. Serdal Sevinc, Veena Kumar, Makonnen Abebe, Susantha Mohottalage, Premkumari Kumarathasan, Renaud Vincent, Hari M. Vijay Protein Expression and Purification.2009; 65(1): 8. CrossRef
Identification of ribosomal phosphoprotein P0 of Neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis Houshuang Zhang, Eung-goo Lee, Min Liao, Muller K.A. Compaore, Guohong Zhang, Osamu Kawase, Kozo Fujisaki, Chihiro Sugimoto, Yoshifumi Nishikawa, Xuenan Xuan Molecular and Biochemical Parasitology.2007; 153(2): 141. CrossRef
Enzyme-catalyzed amplified immunoassay for the detection of Toxoplasma gondii-specific IgG using Faradaic impedance spectroscopy, CV and QCM Yanjun Ding, Hua Wang, Guoli Shen, Ruqin Yu Analytical and Bioanalytical Chemistry.2005; 382(7): 1491. CrossRef
Host cell binding of GRA10, a novel, constitutively secreted dense granular protein from Toxoplasma gondii Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam Biochemical and Biophysical Research Communications.2005; 331(2): 614. CrossRef
The P Domain of the P0 Protein ofPlasmodium falciparumProtects against Challenge with Malaria Parasites K. Rajeshwari, Kalpesh Patel, Savithri Nambeesan, Monika Mehta, Alfica Sehgal, Tirtha Chakraborty, Shobhona Sharma Infection and Immunity.2004; 72(9): 5515. CrossRef
First
Prev
