Parasites, Hosts and Diseases

"tegument"

Brief Communications

Comparative Characterization of Four Calcium-Binding EF Hand Proteins from Opisthorchis viverrini: Palida Emmanoch, Nanthawat Kosa, Suksiri Vichasri-Grams, Smarn Tesana, Rudi Grams, Amornrat Geadkaew-Krenc; Korean J Parasitol 2018;56(1):81-86.
Published online February 28, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.1.81

Four isoforms of calcium binding proteins containing 2 EF hand motifs and a dynein light chain-like domain in the human liver fluke Opisthorchis viverrini, namely OvCaBP1, 2, 3, and 4, were characterized. They had molecular weights of 22.7, 21.6, 23.7, and 22.5 kDa, respectively and showed 37.2-42.1% sequence identity to CaBP22.8 of O. viverrini. All were detected in 2- and 4-week-old immature and mature parasites. Additionally, OvCaBP4 was found in newly excysted juveniles. Polyclonal antibodies against each isoform were generated to detect the native proteins in parasite extracts by Western blot analysis. All OvCaBPs were detected in soluble and insoluble crude worm extracts and in the excretory-secretory product, at approximate sizes of 21-23 kDa. The ion-binding properties of the proteins were analyzed by mobility shift assays with the divalent cations Ca²⁺, Mg²⁺, Zn²⁺, and Cu²⁺. All OvCaBPs showed mobility shifts with Ca²⁺ and Zn²⁺. OvCaBP1 showed also positive results with Mg²⁺ and Cu²⁺. As tegumental proteins, OvCaBP1, 2, and 3 are interesting drug targets for the treatment of opisthorchiasis.

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Evolutionary analysis of species-specific duplications in flatworm genomes
Mauricio Langleib, Javier Calvelo, Alicia Costábile, Estela Castillo, José F. Tort, Federico G. Hoffmann, Anna V. Protasio, Uriel Koziol, Andrés Iriarte
Molecular Phylogenetics and Evolution.2024; 199: 108141. CrossRef
Cystatins from the Human Liver Fluke Opisthorchis viverrini: Molecular Characterization and Functional Analysis
Amornrat Geadkaew-Krenc, Rudi Grams, Sinee Siricoon, Nanthawat Kosa, Dawid Krenc, Wansika Phadungsil, Pongsakorn Martviset
Pathogens.2023; 12(7): 949. CrossRef
Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes
Muhammad Ehsan, Rui-Si Hu, Jun-Ling Hou, Hany M. Elsheikha, Xiao-Dong Li, Pan-Hong Liang, Xing-Quan Zhu
Parasites & Vectors.2021;[Epub] CrossRef

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Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes: Eun-Taek Han, Hyun-Jong Yang, Young-Jin Park, Jeong-Hyun Park, Jong-Yil Chai; Korean J Parasitol 2014;52(2):201-204.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.201

Abstract
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This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species.

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Reliability of heterophyid antigens in heterologous protection against human schistosomiasis
Alaa H. A. Hegazy, Lamia A. Galal, Tasneem M. Hassan, Refaat M. A. Khalifa
Journal of Parasitic Diseases.2020; 44(2): 349. CrossRef
Fishborne zoonotic heterophyid infections: An update
Jong-Yil Chai, Bong-Kwang Jung
Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef

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Electron Microscopy of the Separated Outer Tegument of the Sparganum and Its Antigenicity: Hyun-Jong Yang; Korean J Parasitol 2012;50(2):181-183.
Published online May 24, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.2.181

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The author reported previously on separation of the outer tegument of the spargana (plerocercoids of Spirometra mansoni) using high concentration of urea solution. To determine which layer of the tegument is separated by this method, an electron microscopic analysis has been processed in this study. It was confirmed that the basement layer of the tegument is separated from the parenchyme of the sparganum. In addition, the antigenicity of the separated outer tegument against the human sparganosis patient sera was evaluated. Numerous antigenic proteins, including 16 and 55 kDa proteins, were noticed in the separated tegument; however, there were no diagnostic 31/36 kDa molecules in this tegument. The molecules reactive with the patient sera in the tegument are to be characterized in future studies.

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Separation of the Syncytial Layer of Spargana using Urea: Hyun-Jong Yang; Korean J Parasitol 2009;47(1):69-71.
Published online March 12, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.1.69

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The tegument of tapeworms is known to be composed of an outer syncytial cytoplasm layer which includes microtriches and cytoplasmic organelles (= syncytial layer), and a parenchymatous cytoplasm layer that contains subtegumental cell nuclei (= subtegumental layer) and organelles. In the present study, separation of the syncytial layer of the sparganum, the plerocercoid stage of Spirometra mansoni, was tried using urea as the chemical reagent. Histological sections were prepared to visualize the status of separation after staining with hematoxylin and eosin. The results showed that the syncytial layer of the sparganum tegument which includes microtriches and cytoplasmic organelles were successfully separated from the parenchyma using 3 M urea.

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Electron Microscopy of the Separated Outer Tegument of the Sparganum and Its Antigenicity
Hyun-Jong Yang
The Korean Journal of Parasitology.2012; 50(2): 181. CrossRef
Production of Polyclonal Antibodies against the Tegument of Sparganum (Plerocercoid of Spirometra mansoni) and Its Immunolocalization
Hyun-Jong Yang
The Korean Journal of Parasitology.2010; 48(2): 183. CrossRef

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Original Articles

Tegumental Ultrastructure of Adult Gynaecotyla squatarolae (Digenea: Microphallidae): Do-Seon Lim, Ki-Ju Choi, Sang-Mee Guk, Jong-Yil Chai, Il-Yong Park, Yun-Kyu Park, Min Seo; Korean J Parasitol 2008;46(2):87-90.
Published online June 20, 2008; DOI: https://doi.org/10.3347/kjp.2008.46.2.87

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Gynaecotyla squatarolae (Digenea: Microphallidae) adult flukes were recovered from experimental chicks at day 4-6 post-infection and their tegumental ultrastructure was observed with a scanning electron microscopy. They were pyriform in shape, and their anterior halves were concaved ventrally. The whole body surface was covered with tegumental spines, which were wide and 16-17 digitated between oral and ventral suckers. The density of spines and number of digits decreased posteriorly. The oral sucker was subterminal and the excretory pore was at the posterior end of the worm. Two ventral suckers were similar in appearance and protruded near midline of the worm. The genital atrium was dextral to the small ventral sucker. The dorsal surface was covered with tegumental spines, but the spines were sparser than on the ventral surface. On the middle portion of the dorsal surface, a small opening presumed to be the Laurer's canal was seen. From these findings, it has been confirmed that the adult G. squatarolae has unique characteristics in the surface ultrastructure.

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Two new species of Parspina Pearse, 1920 (Digenea: Cryptogonimidae) from freshwater fishes (Gymnotiformes) of the Paraná River basin in Argentina
Margarita C. Ostrowski de Núñez, Nathalia J. Arredondo, Alicia A. Gil de Pertierra
Systematic Parasitology.2011; 80(1): 67. CrossRef
Ultrastructural features of the tegumental surface of a new metacercaria, Nematostrigea sp. (Trematoda: Strigeidae), with a search for potential taxonomically informative characters
Larisa G. Poddubnaya, Elena Mishina, Alexander E. Zhokhov, David I. Gibson
Systematic Parasitology.2010; 75(1): 59. CrossRef

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Tegumental ultrastructure of the juvenile and adult Himasthla alincia (Digenea: Echinostomatidae): Eun-Taek Han, Kye-Young Han, Jong-Yil Chai; Korean J Parasitol 2003;41(1):17-25.
Published online March 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.1.17

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The tegumental ultrastructure of juvenile and adult Himasthla alincia (Digenea: Echinostomatidae) was observed by scanning electron microscopy. One-, 5- (juveniles) and 20-day-old worms (adults) were harvested from chicks experimentally fed metacercariae from a bivalve, Mactra veneriformis. The juvenile worms were elongated and curved ventrally. The head crown bore 31 collar spines, arranged in a single row. The lip of the oral sucker had 12 paired, and 3 single type I sensory papillae, and the ventral sucker had about 25 type II sensory papillae. The anterolateral surface between the two suckers was densely packed with tegumental spines with 4-7 pointed tips. The adult worms were more elongated and filamentous, and had severe transverse folds over the whole body surface. On the head crown and two suckers, type I and II sensory papillae were more densely distributed than in the juvenile worms. Retractile brush-like spines, with 8-10 digits, were seen on the anterolateral surface, whereas claw-shaped spines, with 2-5 digits, were sparsely distributed posteriorly to the ventral sucker. The cirrus characteristically protruded out, and was armed with small spines distally. The surface ultrastructure of H. alincia was shown to be unique among echinostomes, especially in the digitation of its tegumental spines, the distribution of sensory papillae and by severe folds of the tegument.

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Surface ultrastructure of the adult and juvenile stages of the trematode Astiotrema impletum (Looss, 1899) Looss 1900 (incertae sedis) from the Nile puffer, Tetraodon lineatus Linnaeus, 1758
S. G. Abd El-Kareem, M. H. Ibraheem
Helminthologia.2021; 58(2): 188. CrossRef
Intestinal Flukes Recovered from a Herring Gull, Larus argentatus, in the Republic of Korea
Young-Il Lee, Min Seo, Jong-Yil Chai
The Korean Journal of Parasitology.2020; 58(1): 81. CrossRef
Tegumental ultrastructure of adult Quinqueserialis quinqueserialis (Trematoda: Notocotylidae): an intestinal parasite of muskrat (Ondatra zibethicus)
Soraya Naem, Ashleigh B. Smythe
Parasitology Research.2015; 114(7): 2473. CrossRef
Three Echinostome Species from Wild Birds in the Republic of Korea
Seongjun Choe, Dongmin Lee, Hansol Park, Mihyeon Oh, Hyeong-Kyu Jeon, Youngsun Lee, Ki-Jeong Na, Youngjun Kim, Hang Lee, Keeseon S. Eom
The Korean Journal of Parasitology.2014; 52(5): 513. CrossRef
Topography and ultrastructure of the tegument of Aphallus tubarium (Rodolphi, 1819) Poche, 1926 (Digenea: Cryptogonimidae), intestinal parasite of the common Dentex dentex (Linnaeus 1758) from Valinco Gulf
Laetitia Antonelli, Yann Quilichini, Jos?phine Foata, Bernard Marchand
Acta Parasitologica.2014;[Epub] CrossRef
Echinostoma macrorchis in Lao PDR: Metacercariae in Cipangopaludina Snails and Adults from Experimentally Infected Animals
Woon-Mok Sohn, Jong-Yil Chai, Byoung-Kuk Na, Tai-Soon Yong, Keeseon S. Eom, Hansol Park, Duk-Young Min, Han-Jong Rim
The Korean Journal of Parasitology.2013; 51(2): 191. CrossRef
Topography and ultrastructure of the tegument of Deropristis inflata Molin, 1859 (Digenea: Deropristidae), a parasite of the European eel Anguilla anguilla (Osteichthyes: Anguillidae)
Jean-José Filippi, Yann Quilichini, Bernard Marchand
Parasitology Research.2013; 112(2): 517. CrossRef
Topography and ultrastructure of the tegument of Lecithochirium musculus (digenea: Hemiuridae), a parasite of the European eel Anguilla anguilla (osteichthyes: Anguillidae)
Jean‐José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
Journal of Morphology.2012; 273(4): 361. CrossRef
Surface Ultrastructure of Juvenile and Adult Acanthoparyphium tyosenense (Digenea: Echinostomatidae)
Eun-Taek Han, Moon-Seok Choi, Sung-Yil Choi, Jong-Yil Chai
Journal of Parasitology.2011; 97(6): 1049. CrossRef
Topography and Ultrastructure of the Tegument of Bucephalus anguillae (Digenea: Bucephalidae), a Parasite of the European Eel Anguilla anguilla (Osteichthyen: Anguillidae)
Jean-José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
Journal of Parasitology.2010; 96(6): 1102. CrossRef
Echinostoma caproni: Differential tegumental responses to growth in compatible and less compatible hosts
Javier Sotillo, Alan Trudgett, Liam Halferty, Antonio Marcilla, J. Guillermo Esteban, Rafael Toledo
Experimental Parasitology.2010; 125(3): 304. CrossRef
Centrocestus formosanus (Digenea: Heterophyidae) Encysted in the Freshwater Fish, Puntius brevis, from Lao PDR
Eun-Taek Han, Eun-Hee Shin, Souvanny Phommakorn, Bounthong Sengvilaykham, Jae-Lip Kim, Han-Jong Rim, Jong-Yil Chai
The Korean Journal of Parasitology.2008; 46(1): 49. CrossRef
Mactra veneriformis, an Intertidal Clam, as a New Second Intermediate Host for Acanthoparyphium marilae (Digenea: Echinostomatidae)
Eun-Taek Han, Jong-Yil Chai
The Korean Journal of Parasitology.2008; 46(2): 101. CrossRef
FIRST RECORD AND DESCRIPTION OF METACERCARIAE OF CURTUTERIA ARGUINAE N. SP. (DIGENEA: ECHINOSTOMATIDAE), PARASITE OF COCKLES CERASTODERMA EDULE (MOLLUSCA: BIVALVIA) IN ARCACHON BAY, FRANCE
Céline Desclaux, Fernanda Russell-Pinto, Xavier de Montaudouin, Guy Bachelet
Journal of Parasitology.2006; 92(3): 578. CrossRef

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Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection: Sung-Jong Hong, Tae Yun Kim, Kye-Yong Song, Woon-Mok Sohn, Shin-Yong Kang; Korean J Parasitol 2001;39(4):307-312.
Published online December 31, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.4.307

Abstract
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In the course of Clonorchis sinensis infection, antigens presented to the hosts may be in a close relation to growth of the fluke. The antigenic proteins stimulating IgG antibody production were chronologically identified by immunoblot and localized by immunohistochemical staining. In the early stage of infection until 12 weeks post-infection (PI), antigens were proteins with molecular mass larger than 34 kDa which were derived from the tegument, testes and intrauterine eggs. After 20 weeks PI, antigens recognized were 29, 27 and 26 kDa proteins from the intestine, excretory bladder and reproductive organs. It is suggested that the tegumental proteins are the most potent antigens and the excretory-secretory proteins with middle molecular mass of 26-45 kDa contribute to the high level production of antibodies after 20 weeks of the C. sinensis infection.

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Peng Li, Yongjian Shao, Ke Xu, Xiaohui Qiu
Review of Scientific Instruments.2021;[Epub] CrossRef
Identification of Myoferlin, a Potential Serodiagnostic Antigen of Clonorchiasis, via Immunoproteomic Analysis of Sera From Different Infection Periods and Excretory-Secretory Products of Clonorchis sinensis
Xiao-Xiao Ma, Yang-Yuan Qiu, Zhi-Guang Chang, Jun-Feng Gao, Rui-Ruo Jiang, Chun-Lin Li, Chun-Ren Wang, Qiao-Cheng Chang
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Mapping of the putative epitope domain of Clonorchis sinensis paramyosin (CsPmy) recognized by CsPmy-specific immunoglobulin G in sera of human clonorchiasis
Jung-Mi Kang, Hye-Lim Ju, Jinyoung Lee, Tae Im Kim, Shin-Hyeong Cho, Tong-Soo Kim, Woon-Mok Sohn, Byoung-Kuk Na
Molecular and Biochemical Parasitology.2015; 201(1): 66. CrossRef
Coproantigen capture ELISA for detection of Clonorchis sinensis infection in experimentally infected rats
S.M. Mazidur Rahman, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
Parasitology International.2012; 61(1): 203. CrossRef
Functional Genes and Proteins of Clonorchis sinensis
Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
The Korean Journal of Parasitology.2009; 47(Suppl): S59. CrossRef
Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory‐secretory products of Clonorchis sinensis adult worms
Jung‐Won Ju, Hyun‐Na Joo, Myoung‐Ro Lee, Shin‐Hyeong Cho, Hyeng‐Il Cheun, Jung‐Yeon Kim, Young‐Hee Lee, Kwang‐Jun Lee, Woon‐Mok Sohn, Dong‐Min Kim, Il‐Chul Kim, Byoung Chul Park, Tong‐Soo Kim
PROTEOMICS.2009; 9(11): 3066. CrossRef
A novel tegumental protein 31.8 kDa of Clonorchis sinensis: sequence analysis, expression, and immunolocalization
Yan Huang, Zhenwen Zhou, Xuchu Hu, Quande Wei, Jin Xu, Zhongdao Wu, Xinbing Yu
Parasitology Research.2007; 102(1): 77. CrossRef
Organ-specific antigens of Clonorchis sinensis
Shunyu Li, Byung-Suk Chung, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2004; 42(4): 169. CrossRef
Clinical and epidemiological data of patients with clonorchiasis
Ke-Xia Wang, Rong-Bo Zhang, Yu-Bao Cui, Ye Tian, Ru Cai, Chao-Pin Li
World Journal of Gastroenterology.2004; 10(3): 446. CrossRef
Use of a recombinant Clonorchis sinensis pore-forming peptide, clonorin, for serological diagnosis of clonorchiasis
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Parasitology International.2003; 52(2): 175. CrossRef

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Surface ultrastructure of Metagonimus takahashii metacercariae and adults: Jong-Yil Chai, Sang-Mee Guk, Eun-Taek Han, Min Seo, Eun-Hee Shin, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee; Korean J Parasitol 2000;38(1):9-15.
Published online March 31, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.1.9

Abstract
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A scanning electron microscopic study was performed on the surface ultrastructure of metacercariae and adults of Metagonimus takahashii. Metacercariae were collected from the scale of crucian carp (Carassius auratus), and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker had type I (numerous) and type II (seven in total) sensory papillae. Tegumental spines were dense and digitated into 5-7 points on the surface anterior to the ventral sucker, but became sparse and less digitated posteriorly toward the end of the body. In adults, seven type II sensory papillae were characteristically arranged around the lip of the oral sucker, and on the inner side of the lip four small and two large type I sensory papillae were symmetrically seen on each side (12 in total). Tegumental spines on anterior two-thirds of the body, were digitated with 9-12 tips ventrally and 8-13 tips dorsally. Sperms entering into the Laurer's canal were observed. The results show that the surface ultrastructure of M. takahashii is generally similar to those of M. yokogawai and M. miyatai except for the digitation of tegumental spines.

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Morphological and molecular characterization of Acanthostomum species with Plesiomonas shigelloides coinfection: Immunological and histopathological appraisal in Bagrus bajad
Marwa M. Attia, Abdullah Albaqami, Rania I. Mohamed, Hanim S. Heikal, Asmaa W. Soliman, Mohamed Abdelsalam
Microbial Pathogenesis.2025; 200: 107365. CrossRef
Body Surface Ultrastructure as a Main Morphological Criterion for Distinguishing Adult Trematode Metagonimus suifunensis
Polina Shumenko, Yulia Tatonova, Mikhail Shchelkanov
Biology.2024; 13(11): 942. CrossRef
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Metagonimus yokogawai Ancient DNA Recovered from 16th- to 17th-Century Korean Mummy Feces of the Joseon Dynasty
Jong Ha Hong, Min Seo, Chang Seok Oh, Jong-Yil Chai, Dong Hoon Shin
Journal of Parasitology.2020;[Epub] CrossRef
Light microscopy and surface topography of Urotrema scabridum and Renschetrema indicum (Digenea) from Rhinopoma hardwickii (Chiroptera): first report in Egypt
Kareem Morsy, Fathy Abdel-Ghaffar, Abdel-Rahman Bashtar, Saad Bin Dajem, Rewaida Abdel-Gaber, Marwa Mostafa
Revista Brasileira de Parasitologia Veterinária.2018;[Epub] CrossRef
Topography and ultrastructure of the tegument of Aphallus tubarium (Rodolphi, 1819) Poche, 1926 (Digenea: Cryptogonimidae), intestinal parasite of the common Dentex dentex (Linnaeus 1758) from Valinco Gulf
Laetitia Antonelli, Yann Quilichini, Jos?phine Foata, Bernard Marchand
Acta Parasitologica.2014;[Epub] CrossRef
Porangatus ceteyusn. gen., n. sp. (Digenea: Zoogonidae): A Parasite ofHoplosternum littorale(Pisces: Callichthyidae) from Amazonas State, Brazil
Berenice M. M. Fernandes, José C. O. Malta, Aprigio M. Morias
Comparative Parasitology.2013; 80(2): 203. CrossRef
Topography and ultrastructure of the tegument of Deropristis inflata Molin, 1859 (Digenea: Deropristidae), a parasite of the European eel Anguilla anguilla (Osteichthyes: Anguillidae)
Jean-José Filippi, Yann Quilichini, Bernard Marchand
Parasitology Research.2013; 112(2): 517. CrossRef
Topography and ultrastructure of the tegument of Lecithochirium musculus (digenea: Hemiuridae), a parasite of the European eel Anguilla anguilla (osteichthyes: Anguillidae)
Jean‐José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
Journal of Morphology.2012; 273(4): 361. CrossRef
Surface Ultrastructure of Juvenile and Adult Acanthoparyphium tyosenense (Digenea: Echinostomatidae)
Eun-Taek Han, Moon-Seok Choi, Sung-Yil Choi, Jong-Yil Chai
Journal of Parasitology.2011; 97(6): 1049. CrossRef
Topography and Ultrastructure of the Tegument of Bucephalus anguillae (Digenea: Bucephalidae), a Parasite of the European Eel Anguilla anguilla (Osteichthyen: Anguillidae)
Jean-José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
Journal of Parasitology.2010; 96(6): 1102. CrossRef
The life cycle of Ascocotyle (Phagicola) longa (Digenea: Heterophyidae), a causative agent of fish-borne trematodosis
Susana Balmant Emerique Simões, Helene Santos Barbosa, Cláudia Portes Santos
Acta Tropica.2010; 113(3): 226. CrossRef
TAXONOMIC STATUS, REDESCRIPTION, AND SURFACE ULTRASTRUCTURE OF ASCOCOTYLE (PHAGICOLA) PINDORAMENSIS N. COMB. (DIGENEA: HETEROPHYIDAE)
Susana Balmant Emerique Simões, Tomáš Scholz, Helene Santos Barbosa, Cláudia Portes Santos
Journal of Parasitology.2006; 92(3): 501. CrossRef
Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
The Korean Journal of Parasitology.2004; 42(3): 129. CrossRef
Surface ultrastructure of the adult stage of Acanthotrema felis (Trematoda: Heterophyidae)
Woon-Mok Sohn, Min Seo, Jong-Yil Chai
The Korean Journal of Parasitology.2003; 41(2): 107. CrossRef
Tegumental ultrastructure of Echinoparyphium recurvatum according to developmental stages
Woon-Mok Sohn, Ho-Choon Woo, Sung-Jong Hong
The Korean Journal of Parasitology.2002; 40(2): 67. CrossRef
Surface ultrastructure of Pygidiopsis summa (Digenea: Heterophyidae) adult flukes
Jong-Yil Chai, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee
The Korean Journal of Parasitology.2002; 40(3): 107. CrossRef
Brachylaima cribbi (Digenea: Brachylaimidae): scanning electron microscopical observations of the life-cycle stages
A.R. Butcher, J.K. Brealey, D.I. Grove, R.B. Dymock
Journal of Helminthology.2002; 76(3): 207. CrossRef

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Surface ultrastructure of Metagonimus miyatai metacercariae and adults: Jong-Yil Chai, Young-Je Kang, Sung-Yil Choi, Sang-Mee Guk, Jae-Ran Yu, Soon-Hyung Lee; Korean J Parasitol 1998;36(4):217-225.
Published online December 20, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.4.217

Abstract
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A scanning electron microscopic study was performed to observe surface ultrastructures of excysted metacercariae and adults of Metagonimus miyatai. Metacercariae were collected from the scale of the pale chub (Zacco platypus), and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker was devoid of tegumental spines and had type I and type II sensory papillae. Anteriorly to the ventral sucker, spines were dense and digitated into 5-7 points, whereas near the posterior end of the body spines were sparse and digitated into 2-3 points. In one-week adults, 7 type II sensory papillae were arranged around the lip of the oral sucker, and at inner side of the lip one pair of small and two pairs of large type 1 sensory papillae were seen on each side. The distribution of tegumental spines was similar to that of metacercariae, but they were more differentiated with 9-11 pointed tips. In two- to four-week old adults, the surface ultrastructure was nearly the same as in one-week old adults, however, sperms were frequently seen entering into the Laurer's canal. Conclusively, the surface ultrastructure of M. miyatai was generally similar to that of M. yokogawai, however, differentiation of tegumental spines and distribution of sensory papillae around the oral sucker were different between the two species, which may be of taxonomic significance.

Citations

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Body Surface Ultrastructure as a Main Morphological Criterion for Distinguishing Adult Trematode Metagonimus suifunensis
Polina Shumenko, Yulia Tatonova, Mikhail Shchelkanov
Biology.2024; 13(11): 942. CrossRef
Surface ultrastructure of the adult and juvenile stages of the trematode Astiotrema impletum (Looss, 1899) Looss 1900 (incertae sedis) from the Nile puffer, Tetraodon lineatus Linnaeus, 1758
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