Parasites, Hosts and Diseases

"ticks"

Brief Communication

Molecular detection of Toxoplasma gondii in ticks and their respective host dogs: Min-Goo Seo, Dongmi Kwak; Parasites Hosts Dis 2025;63(1):66-74.
Published online February 25, 2025; DOI: https://doi.org/10.3347/PHD.24091

Abstract
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We identified the presence of Toxoplasma gondii in ticks and their host dogs, and assess the potential role of ticks as reservoirs for this pathogen. A total of 1,230 feeding ticks were collected from 340 dogs. The tick species identified included Haemaphysalis longicornis, H. flava, and Ixodes nipponensis. Detection of the T. gondii B1 gene occurred in 2 dogs (0.6%) and 4 tick pools (0.9%). Genotyping confirmed the presence of the I/III genotype. This study is the first to report the molecular detection of T. gondii in both canine ticks and their hosts. Our findings offer important insights into the dynamics of T. gondii transmission between vectors and their hosts.

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Detection of intestinal parasites in leopard cat fecal samples using shotgun metagenomics
Jun Ho Choi, Singeun Oh, Myung-hee Yi, Dongjun Kang, Du-Yeol Choi, Xavier Chavarria, Arwa Shatta, Yoon Hee Cho, Seongjun Choe, Seung-Hun Lee, Ju Yeong Kim
Parasites, Hosts and Diseases.2025; 63(4): 349. CrossRef

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Original Articles

In vitro anti-Trypanosoma cruzi activity of methanolic extract of Bidens pilosa and identification of active compounds by gas chromatography-mass spectrometry analysis: Gabriel Enrique Cázares-Jaramillo, Zinnia Judith Molina-Garza, Itza Eloisa Luna-Cruz, Luisa Yolanda Solís-Soto, José Luis Rosales-Encina, Lucio Galaviz-Silva; Parasites Hosts Dis 2023;61(4):405-417.
Published online November 28, 2023; DOI: https://doi.org/10.3347/PHD.23069

Abstract
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Chagas disease, caused by Trypanosoma cruzi parasite, is a significant but neglected tropical public health issue in Latin America due to the diversity of its genotypes and pathogenic profiles. This complexity is compounded by the adverse effects of current treatments, underscoring the need for new therapeutic options that employ medicinal plant extracts without negative side effects. Our research aimed to evaluate the trypanocidal activity of Bidens pilosa fractions against epimastigote and trypomastigote stages of T. cruzi, specifically targeting the Brener and Nuevo León strains—the latter isolated from Triatoma gerstaeckeri in General Terán, Nuevo León, México. We processed the plant’s aerial parts (stems, leaves, and flowers) to obtain a methanolic extract (Bp-mOH) and fractions with varying solvent polarities. These preparations inhibited more than 90% of growth at concentrations as low as 800 μg/ml for both parasite stages. The median lethal concentration (LC50) values for the Bp-mOH extract and its fractions were below 500 μg/ml. Tests for cytotoxicity using Artemia salina and Vero cells and hemolytic activity assays for the extract and its fractions yielded negative results. The methanol fraction (BPFC3MOH1) exhibited superior inhibitory activity. Its functional groups, identified as phenols, enols, alkaloids, carbohydrates, and proteins, include compounds such as 2-hydroxy-3-methylbenzaldehyde (50.9%), pentadecyl prop-2-enoate (22.1%), and linalool (15.4%). Eight compounds were identified, with a match confirmed by the National Institute of Standards and Technology (NIST-MS) software through mass spectrometry analysis.

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Inhibitory Activity of Compounds Obtained from Streptomyces Against Trypanosoma cruzi
Jorge Andrés Delgado-Garduño, Lucio Galaviz-Silva, Ma Guadalupe Rojas-Verde, Joel Horacio Elizondo-Luevano, Lidia Baylón-Pacheco, José Luis Rosales-Encina, Guadalupe Gutiérrez-Soto, Zinnia Judith Molina-Garza
Pathogens.2025; 14(7): 638. CrossRef
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Joel H. Elizondo-Luevano, Ramiro Quintanilla-Licea, Sandra L. Castillo-Hernández, Eduardo Sánchez-García, Minerva Bautista-Villarreal, Georgia M. González-Meza, Marcela A. Gloria-Garza, Osvelia E. Rodríguez-Luis, Maciej Ireneusz Kluz, Miroslava Kačániová
Life.2024; 14(9): 1176. CrossRef

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Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in rabbits: Myung-Jo You; Korean J Parasitol 2004;42(4):195-200.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.195

Abstract
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We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of H. longicornis, and identified P27/30 as a troponin I-like protein. In this study, rabbits that were immunized with recombinant P27/30 expressed in Escherichia coli showed the statistically significant longer feeding duration for larval and adult ticks (P<0.05), low engorgement rates in larval ticks (64.4%), and an apparent reduction in egg weights, which suggest that H. longicornis P27/30 protein is a potential candidate antigen for a tick vaccine. These results demonstrated that the recombinant P27/30 protein might be a useful vaccine candidate antigen for biological control of H. longicornis.

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Comprehensive antigen identification and comparative analysis: significant approaches for controlling Haemaphysalis longicornis ticks
Md. Samiul Haque, Bumseok Kim, Myung-Jo You
Journal of Veterinary Science.2025;[Epub] CrossRef
De Novo Transcriptome Sequencing and Profiling of Ovarian Development of Argas persicus Along the Trophogonic Cycle
Fen Yan, Deyong Duan, Jinzhu Meng, Tianyin Cheng
Genes.2025; 16(9): 1107. CrossRef
Hemalin vaccination modulates the host immune response and reproductive cycle of Haemaphysalis longicornis
Hejia Ma, Jingkai Ai, Yansha La, Xinyuan Zhao, Ankang Zeng, Qi Qin, Shangjiali Feng, Ming Kang, Yali Sun, Jixu Li
Veterinary Parasitology.2023; 323: 110051. CrossRef
Molecular cloning, expression and impact of ribosomal protein S-27 silencing in Haemaphysalis longicornis (Acari: Ixodidae)
Md Khalesur Rahman, Bumseok Kim, Myungjo You
Experimental Parasitology.2020; 209: 107829. CrossRef
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Mohamamd Mahdi Ranjbar, Shishir K. Gupta, Khodayar Ghorban, Sedigheh Nabian, Alireza Sazmand, Mohammad Taheri, Sahar Esfandyari, Maryam Taheri
Applied Biochemistry and Biotechnology.2015; 175(1): 323. CrossRef
Identification of the tropomyosin (HL-Tm) in Haemaphysalis longicornis
Meiyuan Tian, Zhancheng Tian, Jin Luo, Junren Xie, Hong Yin, Qiaoying Zeng, Hui Shen, Huiping Chai, Xiaosong Yuan, Fangfang Wang, Guangyuan Liu
Veterinary Parasitology.2015; 207(3-4): 318. CrossRef
Identification of salivary antigenic markers discriminating host exposition between two European ticks: Rhipicephalus sanguineus and Dermacentor reticulatus
Vinh Vu Hai, Lionel Almeras, Stephane Audebert, Matthieu Pophillat, Nathalie Boulanger, Philippe Parola, Didier Raoult, Frederic Pages
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Runhui Zhang, Quwu Jise, Wanpeng Zheng, Yongjun Ren, Xiang Nong, Xuhang Wu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Songjia Lai, Guangyou Yang
Parasites & Vectors.2012;[Epub] CrossRef
Cloning, expression and evaluation of the efficacy of a recombinant Haemaphysalis concinna Hc-23 antigen in rabbits
Yao Bian, Weizhong Chen, Guangyou Yang, Xiaobin Gu, Shuxian Wang, Jiagang Sun, Kaijun Li, Hong Wei
Vaccine.2011; 29(5): 1041. CrossRef
Cloning and characterization of a cDNA clone encoding troponin T from tick Haemaphysalis qinghaiensis (Acari: Ixodidae)
Jinliang Gao, Jianxun Luo, Ruiquan Fan, Guiquan Guan, Volker Fingerle, Chihiro Sugimoto, Noboru Inoue, Hong Yin
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology.2008; 151(3): 323. CrossRef
Molecular characterization of a myosin alkali light chain-like protein, a “concealed” antigen from the hard tick Haemaphysalis qinghaiensis
Jinliang Gao, Jianxun Luo, Ruiquan Fan, Guiquan Guan, Qiaoyun Ren, Miling Ma, Chihiro Sugimoto, Qi Bai, Hong Yin
Veterinary Parasitology.2007; 147(1-2): 140. CrossRef
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A. J. NISBET, J. F. HUNTLEY, A. MacKELLAR, N. SPARKS, R. MCDEVITT
Parasite Immunology.2006; 28(8): 401. CrossRef

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85 Download
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Brief Communication

Two human cases of tick bite caused by Ixodes nipponensis: Jung-Hun Ko, Do-Youn Cho, Byoung-Soo Chung, Suk-Il Kim; Korean J Parasitol 2002;40(4):199-203.
Published online December 30, 2002; DOI: https://doi.org/10.3347/kjp.2002.40.4.199

Abstract
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We report two human cases of tick bite. A 63-year-old male had a pruritic pea-sized brownish nodule on the left popliteal area. Another 41-year-old male had an asymptomatic bean-sized black nodule in the pubic area. The ticks were identified as Ixodes nipponensis, which are the 18th and the 19th cases in Korea.

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D.‐Y. Duan, Z. Chen, Y.‐T. Fu, G.‐H. Liu, Suleman, T.‐Y. Cheng
Medical and Veterinary Entomology.2021; 35(3): 513. CrossRef
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Tian-Yin Cheng, Zhen Chen, Zhong-Bo Li, Guo-Hua Liu
Vector-Borne and Zoonotic Diseases.2018; 18(10): 575. CrossRef
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Yoontae Noh, Yeong Seon Lee, Heung-Chul Kim, Sung-Tae Chong, Terry A. Klein, Ju Jiang, Allen L. Richards, Hae Kyeong Lee, Su Yeon Kim
Parasites & Vectors.2017;[Epub] CrossRef
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