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Metabolism of C14-acetate by some trematodes
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Original Article
Korean J Parasitol. 1965 Dec;3(3):117-121. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1965.3.3.117
Copyright © 1965 by The Korean Society for Parasitology
Metabolism of C14-acetate by some trematodes
Byong Seol Seo,Han Jong Rim,Yong Ok Min,Sang Don Rhee,Tong Hoon Lee and Myong Soon Yun
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Department of Physiology, College of Medicine, Seoul National University, Korea.
Abstract

The adult trematodes, Fasciola hepatica, Eurytrema pancreaticum and Paramphistomum cervi, employed in this experiment were obtained from the cattle slaughtered at the local abbatoir. The worms selected and washed several times in normal sterilized saline solution. Each about ten of intact F. hepatica, fourty of E. pancreaticum, and twenty of P. cervi were incubated in 50 cc volume of special incubation flasks with incubation medium consisting of 10 cc. of Krebs-Ringer phosphate buffer(pH 7.4) The incubation medium was added C14-1-acetate and non-radioactive carrier Na-acetate so as to contain acetate concentration of 50 mg per cent . The worms were allowed to incubate for 5 hours in the Dubnoff metabolic shaking incubator at 38㄃.

After incubation period, respiratory CO2 samples from central well of incubation flask were analysed for total CO2 production rate and their specific activity of respiratory CO2. The lactate and pyruvate appearance rates were determined by analyzing the lactate and pyruvate concentration in a medium after incubation.

The glycogen samples isolated from worms were analyzed for the tissue concentration and their radioactivities in order to determine the turnover rate of glycogen pool. Radioactivities of these series of experiments were counted by an endwindow Geiger-Muller counter as an infinitely thin samples.

The quantitative analysis of C14-acetate utilized by F. hepatica, E. pancreaticum and P. cervi were compared and discussed in this report. According to these data of the experiment, it is suggested that the fatty acid such as acetate may play a part of their oxidative process into the respiratory CO2 and the synthetic process into glycogen in the above species of trematodes.

Tables


Table 1
The oxidation metabolism of C14-acetate into respiratory CO2, lactate and pyruvate by Fasciola hepatica


Table 2
The oxidation metabolism of C14-acetate into respiratory CO2, lactate and pyruvate by Eurytrema pancreaticum


Table 3
The oxidation metabolism of C14-acetate into respiratory CO2, lactate and pyruvate by Paramphistomum cervi


Table 4
The turnover rate of C14-acetate into glycogenby helminths (Trematodes)

References
1. Barker SB, et al. J Biol Chem 1941;138:535–554.
2. von Brand T. Helm Abstr 1960;29(2):97–111.
3. Friedmann TE, et al. J Biol Chem 1943;147:415–421.
4. Good CA, et al. J Biol Chem 1933;485:492.
5. Lee SI. [Metabolism Of C(14)-Lactate By Fasciola Hepatica And Eurytrema Pancreaticum]. Korean J Parasitol 1965;3(1):10–18.
 
6. Seo BS, Rim HJ, Kim KS, Rhee SD, Lim SJ, Lee WS. Metabolism Of C(14)-Glucose By Fasciola Hepatica. Korean J Parasitol 1964;2(3):170–174.
 
7. Seo BS, Rim HJ, Kim KS, Lee MS, Kim YU, Song HY. Metabolism Of C(14)-Glucose By Eurytrema Pancreaticum. Korean J Parasitol 1964;2(3):175–178.
 
8. Seo BS, Rim HJ, Lee SI, Park DK, Moon SC. [Metabolism of C(14)-glucose by Paramphistomum cervi]. Korean J Parasitol 1965;3(1):5–9.
 
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