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Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR

Sang-Eun Lee1, Sung-Hee Hong1, Seong-Ho Lee2, Young-Il Jeong1, Su Jin Lim3, Oh Woong Kwon3, Sun Hyun Kim3, Young Sung You3, Shin-Hyeong Cho1, Won-Ja Lee1
The Korean Journal of Parasitology 2012;50(3):229-231.
Published online: August 13, 2012

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Corresponding author (wonja@nih.go.kr)
• Received: February 7, 2012   • Revised: April 9, 2012   • Accepted: April 14, 2012

© 2012, Korean Society for Parasitology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Toxoplasma gondii is a zoonotic parasite resulting in human infections and one of the infectious pathogens leading to uveitis and retinochoroiditis. The present study was performed to assess T. gondii infection in 20 ocular patients with chronic irregular recurrent uveitis (20 aqueous humor and 20 peripheral blood samples) using PCR. All samples were analyzed by nested PCR targeting a specific B1 gene of T. gondii. The PCR-positive rate was 25% (5/20), including 5% (1) in blood samples, 25% (5) in aqueous humor samples, and 5% (1) in both sample types. A molecular screening test for T. gondii infection in ocular patients with common clinical findings of an unclear retinal margin and an inflammatory membrane over the retina, as seen by fundus examination, may be helpful for early diagnosis and treatment.
  • Key words: Toxoplasma gondii, toxoplasmosis, uveitis, nested PCR, B1 gene
Toxoplasma gondii is an important cause of zoonotic infection worldwide and may lead to uveitis, pneumonia, pericarditis, and neurologic disorders in immunocompetent hosts [1]. As ocular infection is one of the main manifestations of T. gondii infection, ocular toxoplasmosis is one of the most common types of infectious uveitis and a leading cause of posterior uveitis, also called retinochoroiditis [2-4], which may occur either immediately or long after initial infection. Recently, the importance of T. gondii infection in ocular patients has been recognized in view of etiologic and clinical findings. The global prevalence of ocular toxoplasmosis is variable, with 1.8% in Japan, 39.8% in Colombia, 22.2% in Thailand, and 8.4% in the U.S. [2,5-7]. Cases of ocular toxoplasmosis have been reported intermittently since the first case was described in Korea [8-11]. In 2003, T. gondii parasites were successfully isolated from a Korean ocular patient [12]. Recently, clinical features of ocular toxoplasmosis in Korean patients were described by Park et al. [13]. However, general information on ocular toxoplasmosis in Korea is insufficient because clinical reports of ocular toxoplasmosis are rare and no epidemiologic survey has been conducted.
This study was carried out to assess T. gondii infection in 40 clinical samples (20 peripheral blood samples and 20 aqueous humor samples) from ocular patients with irregular recurrent uveitis by PCR. Most of the included patients did not show typical ocular toxoplasmic retinochoroidal lesions, but complained of blurry vision and floaters; common clinical findings included an unclear retinal margin and an inflammatory membrane over the retina, as shown by fundus photography (Figs. 1, 2). Samples were collected by an ophthalmologist from April to August 2009 and transferred from the Nune Eye Hospital to the Division of Malaria and Parasitic Disease at the Korea National Institute of Health for detection of T. gondii. Genomic DNA was extracted from aqueous humor and peripheral blood with the commercially available DNeasy Mini column kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. DNA concentrations were measured using the Quant-iT™ dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA) and read on a Qubit™ Fluorometer (Invitrogen). The average DNA concentration was 8.05 ng/µL (3.3-16.9 ng/µL). The DNA samples were stored at -22℃ until use. Two pairs of oligonucleotide primers, specific to the B1 gene of T. gondii, were used to perform nested PCR using purified blood cell DNA as a template. The published B1 gene sequence was amplified using the Maxime PCR premix Kit (Intron, Seongnam, Korea) according to the method described by Hurtado et al. [14]. The PCR program was modified as follows: 94℃ for 3 min, 15 cycles of 94℃ for 30 sec, 65℃ for 45 sec, 72℃ for 1 min, 35 cycles of 94℃ for 20 sec, 53℃ for 30 sec, 72℃ for 30 sec and a final extension of 72℃ for 5 min.
The overall positive rate for T. gondii detection was 5% (1/20) of blood samples and 25% (5/20) of aqueous humor samples by PCR and 5% (1/20) in both blood and aqueous humor samples (Table 1). This was a little lower than that of other countries [15-17]. It was reported that if aqueous humor samples are withdrawn too early after the onset of symptoms, false-negative by PCR results may be produced [17] or may be attributable to late sampling [18]. Therefore, PCR has previously been shown to be a useful tool for early diagnosis of T. gondii infection in patients who test negative by ELISA [19,20] and PCR detection of parasites within aqueous humor samples formally confirms the diagnosis of either primary or reactivated ocular toxoplasmosis [21].
T. gondii was found in the blood of acutely and chronically infected patients regardless of toxoplasmic retinochoroiditis [20]. Although the examination with light microscopy in peripheral blood samples was not carried out, the specific B1 gene of T. gondii in the blood was identified by nested PCR and confirmed by sequencing. The PCR-positive patients with chronic T. gondii infection in the present study had an irregular recurrence of symptoms, as previously stated, and the infection may thus be regarded as atypical chronic ocular toxoplasmosis. Additionally, the supplementary diagnostic tests for other infections, including syphilis (VDRL), HIV (ELISA), tuberculosis (chest X-ray), and autoimmune diseases, such as Vogt-Koyanagi-Harada disease and Behcet disease, were negative in all patients.
The population with ocular toxoplasmosis vary by geographic location [2,5-7,22]. Moreover, an atypical presentation of ocular toxoplasmosis has been reported [4]. Recently, there has been an increase in the reports of ocular toxoplasmosis, addressing the influence of patient age, frequency of inflammation, clinical features, diagnosis, and patterns of uveitis [7]. Therefore, it is necessary to further study the prevalence of ocular toxoplasmosis in a larger population of ocular patients with typical and atypical uveitis or retinochoroiditis in Korea.
In conclusion, we suggest that PCR analysis for diagnosis of ocular toxoplasmosis may be needed for the earlier diagnosis and treatment of ocular patients presenting with blurry vision and floaters caused by an unclear retinal margin and an inflammatory membrane over the retina.
This work was supported by a grant (4847-302-210-13, 2009) from the Korea National Institute of Health, Republic of Korea.
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Fig. 1
The margin of the retinal vessels and disc was unclear due to the vitreous opacity in the patient.
kjp-50-229-g001.jpg
Fig. 2
Inflammatory membrane over the retina as seen by fundus photography in the patient.
kjp-50-229-g002.jpg
Table 1.
PCR results for blood and aqueous humor samples from 20 patients with irregular recurrent uveitis
Table 1.
Nested PCR
Blood Aqueous humor Both
No. of positive/Total 1/20 5/20 1/20
% 5 25 5

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Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR
Korean J Parasitol. 2012;50(3):229-231.   Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.229
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Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR
Korean J Parasitol. 2012;50(3):229-231.   Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.229
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Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR
Image Image
Fig. 1 The margin of the retinal vessels and disc was unclear due to the vitreous opacity in the patient.
Fig. 2 Inflammatory membrane over the retina as seen by fundus photography in the patient.

Fig. 1

Fig. 2

Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR
Nested PCR
Blood Aqueous humor Both No. of positive/Total 1/20 5/20 1/20 % 5 25 5
Table 1. PCR results for blood and aqueous humor samples from 20 patients with irregular recurrent uveitis

Table 1.

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