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Karyological Studies of Biomphalaria tenagophila (d'Orbigny, 1835) (Gastropoda: Planobidae) from Rio de Janeiro, Brazil

Gab Man Park1, Tai-Soon Yong2
The Korean Journal of Parasitology 2014;52(4):449-451.
Published online: August 29, 2014

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Corresponding author (gmpark@kd.ac.kr)
• Received: May 12, 2014   • Revised: June 24, 2014   • Accepted: June 24, 2014

© 2014, Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • The karyotypes of Biomphalaria tenagophila collected from Rio de Janeiro, Brazil were studied using the air-drying method. Somatic cells of this species had 2n=36. The 18 chromosome pairs were identified and classified into 3 groups. The diploid cell has 7 pairs of metacentric, 8 pairs of submetacentric, and 3 pairs of subtelocentric chromosomes. Observed chromosomes ranged from 2.4 to 6.4 µm, and the total length was 122.3 µm. This is the first report on the chromosome of B. tenagophila.
  • Key words: Biomphalaria tenagophila, karyotype, freshwater snail, Brazil
Biomphalaria tenagophila is a species of air-breathing freshwater snail, an aquatic pulmonate gastropod mollusk in the family Planorbidae. B. tenagophila is a neotropical species, and its native distribution include Peru, Uruguay, Argentina, and southern Brazil [1,2,3,4]. Santos et al. [5] was the first to record B. tenagophila on Ilha Grande, Rio de Janeiro, in Brazil. This species is a medically important pest because of transferring the disease, intestinal schistosomiasis [6]. Intestinal schistosomiasis is the most widespread of all types of schistosomiasis [7,8]. B. tenagophila is an intermediate host and a vector of Schistosoma mansoni [9]. S. mansoni infects about 83.31 million people worldwide [10].
The study of an organism genome at the chromosomal level can be used in differentiating one species from another, i.e., the analysis of chromosome numbers, size, and centromere positions [11,12,13]. In fact, modern cytogenetic techniques have since mid-1990s been adopted for studies of Gastropoda. The subfamily Biomphalarinae has been known to be a conservative group in regard to haploid chromosome numbers 18 (Table 1). In this study, the karyotype of B. tenagophila was studied in order to analyze their genetic relationships.
The 9 specimens used in this study were collected in Museu de Arte Moderna (22°54'48.12"S, 43°10'19.17"W), Rio de Janeiro, Brazil, September 2012, and examined shortly after collection (Fig. 1). The chromosome preparations were made on gonad of the specimens by the usual air-drying method [14]. The prepared slides were observed under an Olympus (BX51) microscope. The identical specimens used for this study have been deposited at the Department of Environmental Medical Biology, Kwandong University College of Medicine, Korea.
A microphotograph of somatic metaphase chromosomes and the karyogram are shown in Fig. 2, and measurements of the chromosomes are presented in Table 2. Fig. 2B shows the karyotype of this species arranged by size. The present cytological preparations showed 10 well-spread mitotic cells on a slide. This species showed a diploid chromosome number of 2n=36, with 7 metacentric, 8 submetacentric, and 3 subtelocentric chromosome pairs. Observed chromosomes ranged from 2.4 to 6.4 µm in length. This species was no inter-specimen variability in chromosome counts. Also, sexual dimorphism of chromosomes was not found in this study.
Most species of the Biomphalaria have been investigated and at present, B. glabrata, B. pfeifferi, B. madagascariensis, B. sudanica, B. tanganyicensis, B. alexandrina, and B. truncatus have a haploid chromosome complement of 18 (Table 1). The chromosomes are relatively small and monomorphic. Conservation of chromosome number has been pointed out for many gastropoda groups [15]. Burch [16], in the 1960s, utilized preparations from the ovo-testis and reported a haploid complement of 18; however, these results were deducted from the meiotic stage, where it was often different to identify each and every chromosome. However, with the development of cytological methods such as hypotonic treatment of tissue samples and pre-treatment with colchicines (air drying technique), clearer deductions were possible on the chromosome number and morphology [11]. The morphology of mitotic metaphase chromosomes has been reported in 10 species of the subfamily Biomphalrinae with n=18 and 2n=36 (Table 1).
The present results revealed that the diploid chromosome number of B. tenagophila is 36. With regard to karyotype analysis in the genus Biomphalaria, only 4 paper about B. glabrata, B. truncates, and B. alexandria has been published [17,18,19]. Though the chromosome number is the same between B. glabrata, B. alexandria, B. truncates, and B. tenagophila, the karyotype is different. B. glabrata and B. alexandria have 10 pairs of metacentric, 4 pairs of submetacentric, 2 pairs of acrocentric, and 2 pairs of telocentric chromosomes, and B. truncatus has 10 metacentric, 4 submetacentric, 2 subtelocentric, and 2 telocentric chromosomes, whereas B. tenagophila has 7 metacentric, 8 submetacentric, and 3 subtelocentric chromosomes. The results of this study on the mitotic metaphase chromosomes provided a much more elucidated observation of the chromosomes of this species. Further studies are needed with various analyses for the investigation of the karyo-systematic evolution, and accumulation of karyological information is very much required in the subfamily Biomphalarinae.

We have no conflict of interest related to this study.

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Fig. 1
Shell of Biomphalaria tenagophila from Rio de Janeiro, Brazil.
kjp-52-449-g001.jpg
Fig. 2
Metaphase chromosomes of Biomphalaria tenagophila (A) and karyotype constructed from A (B).
kjp-52-449-g002.jpg
Table 1.
Chromosome numbers and karyotypes of subfamily Biomphalarinae
Table 1.
Species Haploid no. Karyotypea Source References
Biomphalaria alexandria 18 Egypt Natarajan and Gismann [20]
18 10M+4SM+2A+2T Egypt Abdel-Haleem [19]
18 8M+8SM+2ST Egypt Bakry and Garhy [18]
B. choanomphala 18 Tanzania Burch [16]
B. glabrata 18 Puerto Rico Burch [21]
18 10M+4SM+2A+2T USA Raghunathan [17]
18 10M+4SM+2A+2T Egypt Abdel-Haleem [19]
B. sudanica 18 Sudan Burch [21]
B. tanganyicensis 18 Tanzania Natarajan and Gismann [20]
B. pfeifferi 18 Tanzania South Africa Natarajan and Gismann [20]
B. p. gaudi 18 Liberia, Ghana Natarajan and Gismann [20]
B. p. madagascariensis 18 Madagascar Natarajan and Gismann [20]
B. tenagophila 18 7M+8SM+3ST Brazil This study
B. truncatus 18 10M+4SM+2ST+2T Egypt Bakry and Garhy [18]

aA, acrocentric; M, metacentric; SM, submetacentric; ST, subtelocentric; T, telocentric chromosomes.

Table 2.
Total lengths (μm) and relative lengths of chromosomes of Biomphalaria tenagophila
Table 2.
Chromosome Total length ± SE Relative length ± SE Type
1 6.4 ± 0.21 9.3 ± 0.42 M
2 6.2 ± 0.80 9.0 ± 0.35 M
3 5.5 ± 0.45 8.0 ± 0.63 M
4 5.3 ± 0.11 7.7 ± 0.16 ST
5 4.8 ± 0.43 7.0 ± 0.65 SM
6 4.6 ± 0.19 6.7 ± 0.32 M
7 4.4 ± 0.10 6.4 ± 0.19 SM
8 4.2 ± 0.14 6.1 ± 0.27 M
9 4.0 ± 0.10 5.8 ± 0.54 SM
10 3.9 ± 0.08 5.7 ± 0.35 M
11 3.7 ± 0.23 5.4 ± 0.15 SM
12 3.6 ± 0.07 5.2 ± 0.23 SM
13 3.4 ± 0.13 4.9 ± 0.13 M
14 3.2 ± 0.11 4.7 ± 0.16 SM
15 3.0 ± 0.15 4.4 ± 0.33 SM
16 2.8 ± 0.12 4.1 ± 0.12 ST
17 2.6 ± 0.09 3.8 ± 0.08 SM
18 2.4 ± 0.26 3.4 ± 0.21 ST

Based on measurements from 4 sets of karyotyped cells.

SE, standard error.

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Karyological Studies of Biomphalaria tenagophila (d'Orbigny, 1835) (Gastropoda: Planobidae) from Rio de Janeiro, Brazil
Korean J Parasitol. 2014;52(4):449-451.   Published online August 29, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.4.449
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Karyological Studies of Biomphalaria tenagophila (d'Orbigny, 1835) (Gastropoda: Planobidae) from Rio de Janeiro, Brazil
Korean J Parasitol. 2014;52(4):449-451.   Published online August 29, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.4.449
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Karyological Studies of Biomphalaria tenagophila (d'Orbigny, 1835) (Gastropoda: Planobidae) from Rio de Janeiro, Brazil
Image Image
Fig. 1 Shell of Biomphalaria tenagophila from Rio de Janeiro, Brazil.
Fig. 2 Metaphase chromosomes of Biomphalaria tenagophila (A) and karyotype constructed from A (B).

Fig. 1

Fig. 2

Karyological Studies of Biomphalaria tenagophila (d'Orbigny, 1835) (Gastropoda: Planobidae) from Rio de Janeiro, Brazil
Species Haploid no. Karyotypea Source References Biomphalaria alexandria 18 Egypt Natarajan and Gismann [20] 18 10M+4SM+2A+2T Egypt Abdel-Haleem [19] 18 8M+8SM+2ST Egypt Bakry and Garhy [18] B. choanomphala 18 Tanzania Burch [16] B. glabrata 18 Puerto Rico Burch [21] 18 10M+4SM+2A+2T USA Raghunathan [17] 18 10M+4SM+2A+2T Egypt Abdel-Haleem [19] B. sudanica 18 Sudan Burch [21] B. tanganyicensis 18 Tanzania Natarajan and Gismann [20] B. pfeifferi 18 Tanzania South Africa Natarajan and Gismann [20] B. p. gaudi 18 Liberia, Ghana Natarajan and Gismann [20] B. p. madagascariensis 18 Madagascar Natarajan and Gismann [20] B. tenagophila 18 7M+8SM+3ST Brazil This study B. truncatus 18 10M+4SM+2ST+2T Egypt Bakry and Garhy [18] Chromosome Total length ± SE Relative length ± SE Type 1 6.4 ± 0.21 9.3 ± 0.42 M 2 6.2 ± 0.80 9.0 ± 0.35 M 3 5.5 ± 0.45 8.0 ± 0.63 M 4 5.3 ± 0.11 7.7 ± 0.16 ST 5 4.8 ± 0.43 7.0 ± 0.65 SM 6 4.6 ± 0.19 6.7 ± 0.32 M 7 4.4 ± 0.10 6.4 ± 0.19 SM 8 4.2 ± 0.14 6.1 ± 0.27 M 9 4.0 ± 0.10 5.8 ± 0.54 SM 10 3.9 ± 0.08 5.7 ± 0.35 M 11 3.7 ± 0.23 5.4 ± 0.15 SM 12 3.6 ± 0.07 5.2 ± 0.23 SM 13 3.4 ± 0.13 4.9 ± 0.13 M 14 3.2 ± 0.11 4.7 ± 0.16 SM 15 3.0 ± 0.15 4.4 ± 0.33 SM 16 2.8 ± 0.12 4.1 ± 0.12 ST 17 2.6 ± 0.09 3.8 ± 0.08 SM 18 2.4 ± 0.26 3.4 ± 0.21 ST
Table 1. Chromosome numbers and karyotypes of subfamily Biomphalarinae

A, acrocentric; M, metacentric; SM, submetacentric; ST, subtelocentric; T, telocentric chromosomes.

Table 2. Total lengths (μm) and relative lengths of chromosomes of Biomphalaria tenagophila

Based on measurements from 4 sets of karyotyped cells.

SE, standard error.

Table 1.

Table 2.

PHD : Parasites, Hosts and Diseases

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