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Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice
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Original Article

Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice

Mohammad Reza Mohammadi1, Majid Zeinali1, Sussan K. Ardestani1, Amina Kariminia2
The Korean Journal of Parasitology 2006;44(1):43-48.
Published online: March 20, 2006

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Corresponding author (ardestani@ibb.ut.ac.ir)
• Received: May 17, 2005   • Accepted: November 23, 2005

Copyright © 2006 by The Korean Society for Parasitology

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Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice
Korean J Parasitol. 2006;44(1):43-48.   Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.43
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Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice
Korean J Parasitol. 2006;44(1):43-48.   Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.43
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Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice
Image Image Image
Fig. 1 Scanning densitometry diagram of metacyclic L. major proteins (A) which were resolved on a silver stained 7.5-15% gradient SDS-PAGE (B). The band of 152 kDa is indicated by arrow. Lane 1 is wide range molecular weight standard. Lane 2 is metacyclic L. major proteins.
Fig. 2 Immunoblot analysis of parasite-specific IgG isotypes (IgG1, IgG2a, IgG2b and IgG3) in L. major infected BALB/c (A), and C57BL/6 mice (C). Control mice from each strain are in B; b1-b3. strips, BALB/c control sera, b4-b6, C57BL/6 control sera. Lanes 1-4 show IgG1, IgG2a, IgG3 and IgG4 reactive bands respectively. Arrows indicate the locations of 152 and 140 kDa antigenic bands.
Fig. 3 Total IgG monitoring of sixteen recovered Iranian cutaneous leishmaniasis patients (Lanes 1-16). Normal healthy control is shown on lane 17. The location of 152 and 140 kDa antigenic bands are indicated.

Fig. 1

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Fig. 3

Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice
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