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Original Article

Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea

Weon-Gyu Kho1, Yeong-Hong Park2, Joon-Yong Chung1, Jong-Pil Kim1, Sung-Tae Hong3, Won-Ja Lee4, Tong-Soo Kim4, Jong-Soo Lee4
The Korean Journal of Parasitology 1999;37(4):265-270.
Published online: December 31, 1999

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Corresponding author (wgkho@ijnc.inje.ac.kr)
• Received: May 10, 1999   • Accepted: November 27, 1999

Copyright © 1999 by The Korean Society for Parasitology

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Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea
Korean J Parasitol. 1999;37(4):265-270.   Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.265
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Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea
Korean J Parasitol. 1999;37(4):265-270.   Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.265
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Figure

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Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea
Image Image Image Image Image
Fig. 1 Schematic presentation of the Plasmodium vivax CSP gene. The relative positions of primers for PCR (PVF1, PVR1, PVF2 and PVR2) and sequencing (PVF3 and PVR2) are indicated beneath the map.
Fig. 2 PCR of the PvCSP gene from Korean isolates. PCR products were resolved by electrophoresis on a 1.2% agarose gel and stained with ethidium bromide. M, 100 bp marker; lane 1, SK-A type; lane 2, SK-B type.
Fig. 3 The nucleotide sequences of the PvCSP gene of South Korean isolates compared to the NK and China groups. Repeats are numbered and the sequences read from 5' to 3', starting at the first base of the first numbered tandem repeat to the last codon in the last numbered repeat. The predicted amino acid is given above the nucleotide sequence. The sequences are compared to the NK isolate and CH-5 (Mann et al., 1994).
Fig. 4 Variation in the nucleotide sequences of end of the last 27-bp repeat, 36-bp insert and 12-bp repeat. The sequence of post-repeat region of PvCSP was compared with NK, CH2-7, PH79, VK210, SOL-83, SOL-101, Belem and NYUThai (Mann et al., 1994). The bold numbers indicate 12 bp repeat. Shaded squares reveal identical sites.
Fig. 5 RFLP patterns of the PvCSP gene from Korean isolates. RFLP was performed using PvuII (CAG/CTG) restriction enzyme. M, marker; lane 1, undigested SK-A; lane 2 and 3, digested SK-A; lane 4, undigested SK-B; lane 5 and 6, digested SK-B.

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Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea
PHD : Parasites, Hosts and Diseases

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