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Original Article

Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA

Woon-Mok Sohn1, Ho-Woo Nam2
The Korean Journal of Parasitology 1999;37(4):249-256.
Published online: December 31, 1999

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Corresponding author (howoo@cmc.cuk.ac.kr)
• Received: July 26, 1999   • Accepted: November 3, 1999

Copyright © 1999 by The Korean Society for Parasitology

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Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA
Korean J Parasitol. 1999;37(4):249-256.   Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.249
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Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA
Korean J Parasitol. 1999;37(4):249-256.   Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.249
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Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA
Image Image Image Image Image Image Image
Fig. 1 Western blot positive sera of 26 cases (A to Z) showing typical binding patterns of Toxoplasma gondii infection. Numerals indicat MW markers in kDa.
Fig. 2 Distribution of western blot positive sera by ILAT and ELISA. Each serum was from the same lane in Fig. 1 as labelled alphabetically.
Fig. 3 Distribution of western blot negative sera by ILAT and ELISA. Ten cases (a to j) were located in positive ranges of ILAT titers over 1:32 or ELISA absorbance over 0.275.
Fig. 4 Western blot patterns of 10 cases (a to j) described in Fig. 3. Lane X shows positive bands as a standard.
Fig. 5 Western blot of monoclonal antibodies against RH antigen chosen for sandwich-ELISA. Numerals indicate MW markers in kDa.
Fig. 6 Immunofluorescence images of monoclonal antibodies of Fig. 5 on the extracellular RH (RH column) and the intracellular RH (MeOH and PFA/Tx column). × 1,000.
Fig. 7 Distributional pattern of sandwich-ELISA with various subcellular organelle specific monoclonal antibodies to cat sera. Monoclonal clones were derived from Fig. 5. Hatched boxes indicate the negative sera, blank boxes, false reactive sera of Fig. 4, and cross hatched boxes, positive sera, respectively.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA
mAb clone Cut-off valuea) Absorbance
a h i
Tg563 0.12 0.23 0.37 0.55
Tg505 0.32 0.26 0.59 0.56
Tg605 0.32 0.12 0.35 0.36
Tg556 0.25 0.09 0.38 0.34
Tg737 0.26 0.11 0.44 0.40
Tg695 0.40 0.19 0.41 0.39
Tg786 0.28 0.10 0.30 0.29
Tg621 0.31 0.13 0.34 0.38
Table 1. Sandwich-ELISA absorbance of 3 false reactive cases (lane a, h and i) in Fig. 4.

Cut-off value of each clone was calculated as mean + 3SD of negative sera.

Table 1.

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