Abstract
Subgenus classification of Acanthamoeba remains uncertain.
Twenty-three reference strains of Acanthamoeba including 18 (neo)type-strains were subjected for classification at the subgenus level by riboprinting. PCR/RFLP analysis of 18S rRNA gene (rDNA). On the dendrogram reconstructed on the basis of riboprint analyses, two type-strains (A. astronyxis and A. tubiashi) of morphological group 1 diverged early from the other strains and were quite distinct from each other. Four type-strains of morphological group 3, A.
culbertsoni, A. palestinensis, A. healyi were considered taxonomically valid, but A. pustulosa was regarded as an invalid synonym of A. palestinensis. Strains of morphological group 2 were classified into 6 subgroups.
Among them, A. griffini which has an intron in its 18S rDNA was the most divergent from the remaining strains.
Acanthamoeba castellanii Castellani, A. quina Vil3, A.
lugdunensis L3a, A. polyphaga Jones, A. triangularis SH621, and A. castellanii Ma strains belonged to a subgroup, A.
castellanii complex. However, A. quina and A. lugdunensis were regarded as synonyms of A. castellanii. The Chang strain could be regarded as A. hatchetti. Acanthamoeba mauritaniensis, A. divionensis, A. paradivionensis could be considered as synonyms of A. rhysodes. Neff strain was regarded as A. polyphaga rather than as A. castellanii. It is likely that riboprinting can be applied for rapid identification of Acanthamoeba isolated from the clinical specimens and environments.
Citations
Citations to this article as recorded by
- The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis
Min Seung Kang, Sangyoon Kim, Da Som Kim, Hak Sun Yu, Ji Eun Lee, Yi Cao
PLOS ONE.2023; 18(2): e0281141. CrossRef - Acanthamoeba Mannose and Laminin Binding Proteins Variation across Species and Genotypes
Daniele Corsaro
Microorganisms.2022; 10(11): 2162. CrossRef - Acanthamoeba mauritaniensis genotype T4D: An environmental isolate displays pathogenic behavior
Daniel Coronado-Velázquez, Angélica Silva-Olivares, Federico Castro-Muñozledo, Luis Fernando Lares-Jiménez, Libia Zulema Rodríguez-Anaya, Mineko Shibayama, Jesús Serrano-Luna
Parasitology International.2020; 74: 102002. CrossRef - Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)
Stas Malavin, Lyubov Shmakova
European Journal of Protistology.2020; 73: 125671. CrossRef - Species, Sequence Types and Alleles: Dissecting Genetic Variation in Acanthamoeba
Paul A. Fuerst, Gregory C. Booton
Pathogens.2020; 9(7): 534. CrossRef - Adhesion ofAcanthamoebaon Cosmetic Contact Lenses
Seung-Mok Lee, Ji-Eun Lee, Da-In Lee, Hak-Sun Yu
Journal of Korean Medical Science.2018;[Epub] CrossRef - Adhesion of Acanthamoeba on Silicone Hydrogel Contact Lenses
Ga-Hyun Lee, Ji-Eun Lee, Mi-Kyung Park, Hak-Sun Yu
Cornea.2016; 35(5): 663. CrossRef - Effects of multipurpose solutions on the adhesion of Acanthamoeba to rigid gas permeable contact lenses
Ga‐Hyun Lee, Hak‐Sun Yu, Ji‐Eun Lee
Ophthalmic and Physiological Optics.2016; 36(2): 93. CrossRef - Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
The Korean Journal of Parasitology.2013; 51(4): 401. CrossRef - Molecular Phylogeny of Acanthamoeba
Hyun Hee Kong
The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef - Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea
Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu
The Korean Journal of Parasitology.2009; 47(4): 337. CrossRef - Fifty Years of the Korean Society for Parasitology
Seung-Yull Cho
The Korean Journal of Parasitology.2009; 47(Suppl): S7. CrossRef - Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef - Cysticidal Effect on Acanthamoeba and Toxicity on Human Keratocytes by Polyhexamethylene Biguanide and Chlorhexidine
Ji-Eun Lee, Boo Sup Oum, Hee Young Choi, Hak Sun Yu, Jong Soo Lee
Cornea.2007; 26(6): 736. CrossRef - Acanthamoeba: Keratopathogenicity of isolates from domestic tap water in Korea
Hae Jin Jeong, Sun Joo Lee, Jeong Hwan Kim, Ying Hua Xuan, Keun Hee Lee, Sang Kyun Park, Sun Hee Choi, Dong Il Chung, Hyun Hee Kong, Mee Sun Ock, Hak Sun Yu
Experimental Parasitology.2007; 117(4): 357. CrossRef - Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear
Sun Joo Lee, Hae Jin Jeong, Ji Eun Lee, Jong Soo Lee, Ying Hua Xuan, Hyun-Hee Kong, Dong-Il Chung, Mee-Sun Ock, Hak Sun Yu
The Korean Journal of Parasitology.2006; 44(4): 313. CrossRef - Genetic diversity of Acanthamoeba isolates from ocean sediments
Hua Liu, Young-Ran Ha, Sung-Tae Lee, Yean-Chul Hong, Hyun-Hee Kong, Dong-Il Chung
The Korean Journal of Parasitology.2006; 44(2): 117. CrossRef - Protists in soil ecology and forest nutrient cycling
M S Adl, VV SR Gupta
Canadian Journal of Forest Research.2006; 36(7): 1805. CrossRef - Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses
Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung
The Korean Journal of Parasitology.2005; 43(1): 7. CrossRef - Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals
Frederick L. Schuster, Govinda S. Visvesvara
International Journal for Parasitology.2004; 34(9): 1001. CrossRef - Necrotizing Meningoencephalitis and Pneumonitis in a Simian Immunodeficiency Virus—infected Rhesus Macaque due to Acanthamoeba
S. V. Westmoreland, J. Rosen, J. MacKey, C. Romsey, D.-L. Xia, G. S. Visvesvera, K. G. Mansfield
Veterinary Pathology.2004; 41(4): 398. CrossRef - Acanthamoeba sohi, n. sp., a pathogenic Korean isolate YM-4 from a freshwater fish
Kyung-il Im, Ho-Joon Shin
The Korean Journal of Parasitology.2003; 41(4): 181. CrossRef - Acanthamoeba keratitis update—incidence, molecular epidemiology and new drugs for treatment
D V Seal
Eye.2003; 17(8): 893. CrossRef - Acanthamoebaspp. as Agents of Disease in Humans
Francine Marciano-Cabral, Guy Cabral
Clinical Microbiology Reviews.2003; 16(2): 273. CrossRef - A riboprinting scheme for identification of unknown Acanthamoeba isolates at species level
Hyun-Hee Kong, Dong-Il Chung
The Korean Journal of Parasitology.2002; 40(1): 25. CrossRef - Mitochondrial DNA Restriction Fragment Length Polymorphism (RFLP) and 18S Small-Subunit Ribosomal DNA PCR-RFLP Analyses ofAcanthamoebaIsolated from Contact Lens Storage Cases of Residents in Southwestern Korea
Hyun-Hee Kong, Ji-Yeol Shin, Hak-Sun Yu, Jin Kim, Tae-Won Hahn, Young-Ho Hahn, Dong-Il Chung
Journal of Clinical Microbiology.2002; 40(4): 1199. CrossRef - Genetic analyses of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea
Hak-Sun Yu, Kyung-Hee Choi, Hyo-Kyung Kim, Hyun-Hee Kong, Dong-Il Chung
The Korean Journal of Parasitology.2001; 39(2): 161. CrossRef - Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge
Jill M. Schroeder, Gregory C. Booton, John Hay, Ingrid A. Niszl, David V. Seal, Miles B. Markus, Paul A. Fuerst, Thomas J. Byers
Journal of Clinical Microbiology.2001; 39(5): 1903. CrossRef - The Increasing Importance of Acanthamoeba Infections1
FRANCINE MARCIANO‐CABRAL, ROBYN PUFFENBARGER, GUY A. CABRAL
Journal of Eukaryotic Microbiology.2000; 47(1): 29. CrossRef - Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene
Hak-sun Yu, Mee-yul Hwang, Tae-ook Kim, Ho-cheol Yun, Tae-ho Kim, Hyun-hee Kong, Dong-il Chung
The Korean Journal of Parasitology.1999; 37(3): 181. CrossRef - Isoenzyme patterns and phylogenetic relationships in Acanthamoeba spp. isolated from contact lens containers in Korea
Ho-Joon Shin, Myung-Soo Cho, Han-jip Kim, Kyung-il Im
The Korean Journal of Parasitology.1999; 37(4): 229. CrossRef -
Fluorescent Oligonucleotide Probes for Clinical and Environmental Detection of
Acanthamoeba
and the T4 18S rRNA Gene Sequence Type
Diane R. Stothard, John Hay, Jill M. Schroeder-Diedrich, David V. Seal, Thomas J. Byers
Journal of Clinical Microbiology.1999; 37(8): 2687. CrossRef
