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1Department of Parasitology, College of Medicine, Kon-Kuk University, Choongju 380-701,Korea 2Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea
Actin and some actin binding proteins such as tropomyosin, -actinin and troponin T were localized by simultaneous double immunogold labeling in several developmental stages of Cryptosporidium parvum. All of the observed developmental stages have many particles of tropomyosin and actin around pellicle and cytoplasm.
Tropomyosin was labeled much more than the actin when these two proteins were labeled simultaneously. And alpha-actinin was labeled mostly in the pellicle, but troponin T labeling was very rarely observed. From this study, it was suggested that tropomyosin seems to be one of the major proteins of C.
parvum, so it must be playing important roles in C. parvum.
Labeling surface epitopes to identify Cryptosporidium life stages using a scanning electron microscopy-based immunogold approach Hanna Edwards, R.C. Andrew Thompson, Wan H. Koh, Peta L. Clode Molecular and Cellular Probes.2012; 26(1): 21. CrossRef
Distribution of actin and tropomyosin in Cryptosporidium muris J R Yu The Korean Journal of Parasitology.1998; 36(4): 227. CrossRef
