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Biochemical and molecular characterization of a strain KA/S2 of Acanthamoeba castellanii isolated from Korean soil

Chung, D I , Kong, H H , Yu, H S , Oh, Y M , Yee, S T , Lim, Y J
Korean J Parasitol 1996;34(1):79-85.
Department of Parasitology, Kyungpook National University School of Medicine, Taegu, Korea.
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Abstract

A strain, KA/S2, isolated from Korean soil and morphologically assigned to Acanthamoeba castellanii, was characterized by isoenzyme analysis, and total proteins profile, and mitochondrial (Mt) DNA restriction fragment length polymorphism (RFLP), and compared with four reference strains assigned to the species (the authenitic Castellani, Neff, Ma, and Chang strains). It was found that four isoenzyme, total proteins, and Mt DNA RFLP patterns by eight restriction endonucleases of the strain KA/S2 were identical with those of the Neff strain, isolated from soil of California, USA. The Chang strain was unique in its morphology and total protein patterns. Interstrain polymorphisms of isoenzyme profiles and Mt DNA RFLP patterns were observed among the Castellani, Neff, Ma, and Chang strains. Mt DNA RFLP was confirmed to be highly appropriate for the strain characterization and identification of Acanthamoeba spp.

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Biochemical and molecular characterization of a strain KA/S2 of Acanthamoeba castellanii isolated from Korean soil
Korean J Parasitol. 1996;34(1):79-85.
DOI: https://doi.org/10.3347/kjp.1996.34.1.79
Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
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Biochemical and molecular characterization of a strain KA/S2 of Acanthamoeba castellanii isolated from Korean soil
Korean J Parasitol. 1996;34(1):79-85.
DOI: https://doi.org/10.3347/kjp.1996.34.1.79
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