Abstract
In the present communication, YS-27, a Korean strain of Entamoeba histolytica is described for the isolation and establishment of axenic cultivation. E. histolytica, designated as strain "YS-27", was isolated from the pus of a hepatic abscess obtained from a 72 year old inpatient of August 10, 1969. Specimens, were obtained by needle aspiration, inoculated immediately and weekly cultured in a modified diphasic medium at 37 degrees C. Strain YS-27 had been maintained for more than 15 years by weekly subculture until February, 1985. These cultures were transferred to a monoxenic TTY-SB medium seeded with a trypanosomatid of the genus Crithidia. Penicillin G, 2 to 10 x 10(3) International units and Streptomycin, 2 to 10 mg per 100 ml, were added to the cultures to eliminate the bacteria. After more than one year later, these two organisms were well maintained by transfer every 3 or 4 days until January, 1986 at 37 degrees C in TTY-SB medium in the absence of other microorganisms.
These monoxenic cultures were then transferred to TYI-S-33 medium. Strain YS-27 alone had not been growing at the time of transfer, but when overlaid with Crithidia at intervals of 3 to 4 days, strain YS-27 propagated well. The Crithidia died out several weeks later after several passages.
Beginning in April, 1986, strain YS-27, was successfully established in axenic culture in TYI-S-33 medium and has been maintained in continuous culture and multiplied well to present.
Citations
Citations to this article as recorded by
- Mouse models of amoebiasis and culture methods of amoeba
Sharmina Deloer, Risa Nakamura, Fumika Mi-ichi, Keishi Adachi, Seiki Kobayashi, Shinjiro Hamano
Parasitology International.2016; 65(5): 520. CrossRef - Changing Patterns of Human Parasitic Infection in Korea
Myoung-Hee Ahn
Hanyang Medical Reviews.2010; 30(3): 149. CrossRef - Serodiagnosis of amoebiasis using a recombinant protein fragment of the 29 kDa surface antigen of Entamoeba histolytica
Jongweon Lee, Soon-Jung Park, Tai-Soon Yong
International Journal for Parasitology.2000; 30(14): 1487. CrossRef - Characterization of YS-27, an axenic Korean strain of Entamoeba histolytica
Soon-jung Park, Tai-soon Yong, Jae-kyung Chang, Kyung-il Im, Chin-thack Soh
The Korean Journal of Parasitology.1999; 37(1): 59. CrossRef - Synergy between Entamoeba histolytica and Escherichia coli in the induction of cytokine gene expression in human colon epithelial cells
Jung Mogg Kim, Hyun Chae Jung, Kyung-il Im, In Sung Song, Chung Yong Kim
Parasitology Research.1998; 84(6): 509. CrossRef - cDNAs encoding the antigenic proteins in pathogenic strain of Entamoeba histolytica
K I Im, J T Choi, Y P Hong, T E Kim, M S La
The Korean Journal of Parasitology.1997; 35(3): 203. CrossRef - Differentiation of Korean isolates of Entamoeba histolytica from Entamoeba dispar
S C Choe, M Lee, S K Lee, K Im, E Tannich, S H Lee, S T Hong
The Korean Journal of Parasitology.1996; 34(1): 15. CrossRef - Differentiation of Entamoeba histolytica and Entamoeba dispar in cyst-passers by immunoblot
Me jeong LEE, Sung Tae HONG
The Korean Journal of Parasitology.1996; 34(4): 247. CrossRef - Interleukin-8 gene expression in the human colon epithelial cell line, HT-29, exposed to Entamoeba histolytica
J M Kim, H C Jung, K Im, Y J Cho, C Y Kim
The Korean Journal of Parasitology.1995; 33(4): 357. CrossRef
