Warning: fopen(/home/virtual/parasitol/journal/upload/ip_log/ip_log_2025-12.txt): failed to open stream: Permission denied in /home/virtual/lib/view_data.php on line 83
Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 Interleukin-8 gene expression in the human colon epithelial cell line, HT-29, exposed to Entamoeba histolytica
The protozoan parasite, Entamoeba histolytica, is one of major causative agents of intestinal disease all over the world. In acute experimental infection, the early host response to E. histolytica is characterized by an infiltration of neutrophils. However, the chemotactic signal for this response is not well known. Based on the finding that human epithelial cells produce the potent neutrophil chemoattractant and activator, interleukin-8 (IL-8), IL-8 gene expression was examined thoroughly in human colon epithelial cells exposed to E. histolytica trophozoites.
Cellular RNAs were extracted from HT-29 or Caco-2 human colon epithelial cells exposed to E. histolytica trophozoites for 30 minutes, 1 and 3 hours. IL-8 mRNA transcripts were measured by reverse transcriptional polymerase chain reaction (RT-PCR) using synthetic standard RNA. The number of IL-8 mRNA molecules increased from 30 minutes to 3 hours of exposure period, reaching 3.1 x 10(7) molecules/micrograms of total RNA. Expression pattern of IL-8 mRNA transcripts was parallel to the amounts of IL-8 protein measured by enzyme-linked immunosorbent assay (ELISA). Lysates of E. histolytica also induced expression of mRNA for IL-8 in colon epithelial cells. These results suggest that acute inflammatory reaction by E. histolytica may be initially triggered by proinflammatory cytokines such as IL-8 secreted from epithelial cells of the colon.
Citations
Citations to this article as recorded by
Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin The Korean Journal of Parasitology.2022; 60(3): 155. CrossRef
Calpain-dependent cleavage of SHP-1 and SHP-2 is involved in the dephosphorylation of Jurkat T cells induced byEntamoeba histolytica K. A. KIM, Y. A. LEE, M. H. SHIN Parasite Immunology.2010; 32(3): 176. CrossRef
Fifty Years of the Korean Society for Parasitology Seung-Yull Cho The Korean Journal of Parasitology.2009; 47(Suppl): S7. CrossRef
Expression of immune modulator cytokines in human fulminant amoebic colitis R. E. SIERRA‐PUENTE, R. CAMPOS‐RODRÍGUEZ, R. A. JARILLO‐LUNA, L. MUÑOZ‐FERNÁNDEZ, M. G. RODRÍGUEZ, M. H. MUÑOZ‐ORTEGA, J. VENTURA‐JUÁREZ Parasite Immunology.2009; 31(7): 384. CrossRef
Differential Induction of Immunoregulatory Circuits of Phagocytic Cells by Gal/Gal NAc Lectin from Pathogenic and Nonpathogenic Entamoeba Monika Sharma, Deepak Bhasin, Harpreet Vohra Journal of Clinical Immunology.2008; 28(5): 542. CrossRef
Calpain-dependent calpastatin cleavage regulates caspase-3 activation during apoptosis of Jurkat T cells induced by Entamoeba histolytica Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin International Journal for Parasitology.2007; 37(11): 1209. CrossRef
Hematological effects of Blastocystis hominis infection in male foreign workers in Taiwan H. S. Cheng, Y. L. Guo, J. W. Shin Parasitology Research.2003; 90(1): 48. CrossRef
Adhesion of Lactic Acid Bacteria to Caco‐2 Cells and Their Effect on Cytokine Secretion Hirotsugu Morita, Fang He, Tetsuo Fuse, Arthur C. Ouwehand, Hideo Hashimoto, Masataka Hosoda, Koko Mizumachi, Jun‐ichi Kurisaki Microbiology and Immunology.2002; 46(4): 293. CrossRef
Pathophysiology of amoebiasis Samuel L Stanley Trends in Parasitology.2001; 17(6): 280. CrossRef
Proteolytic activity of cysteine protease in excretory–secretory product of Paragonimus westermani newly excysted metacercariae pivotally regulates IL‐8 production of human eosinophils Myeong Heon Shin, Soo Young Lee Parasite Immunology.2000; 22(10): 529. CrossRef
Human intestinal epithelial cells produce proinflammatory cytokines in response to infection in a SCID mouse-human intestinal xenograft model of amebiasis K B Seydel, E Li, P E Swanson, S L Stanley Infection and Immunity.1997; 65(5): 1631. CrossRef
