Abstract
Paragonimus westermani is a common fluke in Korea. The present study aimed to determine serum total IgE and specific IgG levels in experimental paragonimiasis of rats.
Each Wistar rat was inoculated orally with 20-25 metacercariae of P. westermani from Cambaroides similis.
Before and after infection (1, 2, 3, 4, 6, 8 weeks) of P.
westermani, the blood was collected from the retro-orbital venous plexus of rats and kept serum at -70 degrees C. Serum total IgE and specific IgG levels were determined by the capture and conventional enzyme-linked immunosorbent assay, respectively. The results were as follows; 1. Serum IgE values were increased to 0.18 +/- 0.042 at 2 weeks, 0.28 +/- 0.151 at 4 weeks and 0.43 +/- 0.055 at 8 weeks after infection. The absorbances of non-infected rats ranged 0.07 +/- 0.021-0.12 +/- 0.025. 2. Specific IgG values were slightly increased at 3 weeks (0.20 +/- 0.032) and gradually increased up to 8 weeks (0.31 +/- 0.067) after infection.
The absorbances of non-infected rats ranged 0.11 +/- 0.035-0.18 +/- 0.019. The present results suggested that P.
westermani could elevate serum IgE and specific IgG antibodies in Wistar rats which were not a good definitive host.
Citations
Citations to this article as recorded by
- Effects of anti-IgE mAb on serum IgE, FcεRII/CD23 expression on splenic B cells and worm burden in mice infected with Paragonimus westermani
M H Shin, H K Min
The Korean Journal of Parasitology.1997; 35(1): 47. CrossRef - Changes of IgE production, splenic helper and suppressor T lymphocytes in mice infected with Paragonimus westermani
D Y Min, J S Ryu, M H Shin
The Korean Journal of Parasitology.1993; 31(3): 231. CrossRef
