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Volume 51(6); December 2013

Preface

Preface
Thawalwong Ratanasiri
Korean J Parasitol 2013;51(6):611-611.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.611
  • 4,561 View
  • 64 Download

Mini Reviews

Comprehensive Review of Ocular Angiostrongyliasis with Special Reference to Optic Neuritis
Ying Feng, Yukifumi Nawa, Kittisak Sawanyavisuth, Zhiyue Lv, Zhong-Dao Wu
Korean J Parasitol 2013;51(6):613-619.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.613

Angiostrongyliasis, caused by Angiostrongylus cantonensis infection, is a food-borne parasitic disease. Its larvae evoke eosinophilic inflammation in the central nervous system, but can also cause pathological changes in the eyes. Among ocular angiostrongyliasis cases, the incidence of optic neuritis is low and only few sporadic reports exist. Some patients with optic neuritis developed obvious hypopsia or even vision loss, which would seriously influence the quality of life of patients. Prompt treatment of optic neuritis caused by A. cantonensis is the key factor for minimizing the incidence of serious complications of this disease. In this review, we first provide a comprehensive overview of ocular angiostrongyliasis, and then focus on the clinical features of optic neuritis caused by A. cantonensis.

Citations

Citations to this article as recorded by  Crossref logo
  • Seroprevalence of Angiostrongylus cantonensis in suspected human angiostrongyliasis cases and clinical manifestations in a tertiary care hospital in Thailand: a retrospective 18-year longitudinal study
    Lakkhana Sadaow, Thidarat K. Prasongdee, Phuangphaka Sadee, Sureeporn Naonongwai, Patcharaporn Boonroumkaew, Rutchanee Rodpai, Oranuch Sanpool, Amnat Kitkhuandee, Verajit Chotmongkol, Kittisak Sawanyawisuth, Pewpan M. Intapan, Wanchai Maleewong
    Food and Waterborne Parasitology.2025; 41: e00290.     CrossRef
  • Infectious eye disease in the 21st century—an overview
    Gerry Clare, John H. Kempen, Carlos Pavésio
    Eye.2024; 38(11): 2014.     CrossRef
  • An Overview of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae), an Emerging Cause of Human Angiostrongylosis on the Indian Subcontinent
    Divakaran Pandian, Tomáš Najer, David Modrý
    Pathogens.2023; 12(6): 851.     CrossRef
  • Identification and genetic characterization of Angiostrongylus cantonensis isolated from the human eye
    Abdulhakam Dumidae, Kanin Luangsawang, Aunchalee Thanwisai, Apichat Vitta
    Parasitology Research.2023; 122(9): 2217.     CrossRef
  • Clinical Course and Optical Coherence Tomography of Ocular Angiostrongyliasis: A Case Report
    Kanin Luangsawang, Veeraphatra Wongsantimeth, Sirinan Treeyawedkul
    Journal of Current Ophthalmology.2023; 35(1): 86.     CrossRef
  • Clinical Efficacy and Safety of Albendazole and Other Benzimidazole Anthelmintics for Rat Lungworm Disease (Neuroangiostrongyliasis): A Systematic Analysis of Clinical Reports and Animal Studies
    John Jacob, Argon Steel, Zhain Lin, Fiona Berger, Katrin Zöeller, Susan Jarvi
    Clinical Infectious Diseases.2022; 74(7): 1293.     CrossRef
  • Stat3/IL-6 signaling mediates sustained pneumonia induced by Agiostrongylus cantonensis
    Hongli Zhou, Yuting Lu, Hang Wei, Yixin Chen, Yanin Limpanon, Paron Dekumyoy, Ping Huang, Peiyao Shi, Zhiyue Lv, Alessandra Morassutti
    PLOS Neglected Tropical Diseases.2022; 16(5): e0010461.     CrossRef
  • Neuroangiostrongyliasis: Global Spread of an Emerging Tropical Disease
    Robert H. Cowie, Vernon Ansdell, Claire Panosian Dunavan, Randi L. Rollins
    The American Journal of Tropical Medicine and Hygiene.2022; 107(6): 1166.     CrossRef
  • An unusual cause of visual loss: Subfoveal, motile angiostrongyliasis
    Roshni Sawh, Lizette Mowatt
    Tropical Doctor.2021; 51(3): 441.     CrossRef
  • Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Ocular Infections
    Sixto M. Leal, Kyle G. Rodino, W. Craig Fowler, Peter H. Gilligan
    Clinical Microbiology Reviews.2021;[Epub]     CrossRef
  • Cluster of Angiostrongyliasis Cases Following Consumption of Raw Monitor Lizard in the Lao People’s Democratic Republic and Review of the Literature
    Leeyounjera Yang, Chirapha Darasavath, Ko Chang, Vilayvanh Vilay, Amphonesavanh Sengduangphachanh, Aphaphone Adsamouth, Manivanh Vongsouvath, Valy Keolouangkhot, Matthew T. Robinson
    Tropical Medicine and Infectious Disease.2021; 6(3): 107.     CrossRef
  • Eosinophilic meningitis due to Angiostrongylus cantonensis in Europe
    Frederik Federspiel, Sofie Skovmand, Sigurdur Skarphedinsson
    International Journal of Infectious Diseases.2020; 93: 28.     CrossRef
  • Two Ocular Angiostrongyliasis Cases in Thailand with Molecular Identification of Causative Parasite Species
    Patcharaporn Boonroumkaew, Thuss Sanguansak, Siriraksa Visaetsilpanonta, Oranuch Sanpool, Lakkhana Sadaow, Pewpan M. Intapan, Wanchai Maleewong
    The American Journal of Tropical Medicine and Hygiene.2020; 102(6): 1399.     CrossRef
  • A Case Report of Slug Ingestion Causing Eosinophilic Meningitis, Papilledema, and Pronounced Motor Weakness in a US Marine
    Jared R Widder, Shamis Fallah, Todd J Mondzelewski
    Military Medicine.2019;[Epub]     CrossRef
  • Genetic characterization of Angiostrongylus larvae and their intermediate host, Achatina fulica, in Thailand
    Abdulhakam Dumidae, Pichamon Janthu, Chanakan Subkrasae, Paron Dekumyoy, Aunchalee Thanwisai, Apichat Vitta, Marcello Otake Sato
    PLOS ONE.2019; 14(9): e0223257.     CrossRef
  • Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen
    JOEL BARRATT, DOUGLAS CHAN, INDY SANDARADURA, RICHARD MALIK, DEREK SPIELMAN, ROGAN LEE, DEBORAH MARRIOTT, JOHN HARKNESS, JOHN ELLIS, DAMIEN STARK
    Parasitology.2016; 143(9): 1087.     CrossRef
  • Central nervous system manifestations of Angiostrongylus cantonensis infection
    Yuri C. Martins, Herbert B. Tanowitz, Kevin R. Kazacos
    Acta Tropica.2015; 141: 46.     CrossRef
  • Emergence of Neural Angiostrongyliasis in Eastern Australia
    Mahdis Aghazadeh, Malcolm K. Jones, Kieran V. Aland, Simon A. Reid, Rebecca J. Traub, James S. McCarthy, Rogan Lee
    Vector-Borne and Zoonotic Diseases.2015; 15(3): 184.     CrossRef
  • Effects of albendazole combined with TSII-A (a Chinese herb compound) on optic neuritis caused by Angiostrongylus cantonensis in BALB/c mice
    Feng Feng, Ying Feng, Zhen Liu, Wei-Hua Li, Wen-Cong Wang, Zhong-Dao Wu, Zhiyue Lv
    Parasites & Vectors.2015;[Epub]     CrossRef
  • A Case of Ocular Angiostrongyliasis with Molecular Identification of the Species in Vietnam
    Nguyen Van De, Le Van Duyet, Jong-Yil Chai
    The Korean Journal of Parasitology.2015; 53(6): 713.     CrossRef
  • Animal model of human disease with optic neuritis: neuropapillitis in a rat model infected with Angiostrongylus cantonensis
    Ying Feng, Xin Zeng, Wei-hua Li, Wen-cong Wang, Li-si Ou-Yang, Xi Sun, Zhiyue Lv, Zhong-Dao Wu
    Parasitology Research.2014; 113(11): 4005.     CrossRef
  • The pathogenesis of optic neuritis caused by Angiostrongylus cantonensis in BALB/c mice
    Ying Feng, Xin Zeng, Wei-Hua Li, Wen-Cong Wang, Wei Chen, Li-si Ou-Yang, Xi Sun, Feng Feng, Zhong-Dao Wu
    Parasites & Vectors.2014;[Epub]     CrossRef
  • 10,638 View
  • 92 Download
  • Crossref
Paragonimus and Paragonimiasis in Vietnam: an Update
Pham Ngoc Doanh, Yoichiro Horii, Yukifumi Nawa
Korean J Parasitol 2013;51(6):621-627.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.621

Paragonimiasis is a food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. In Vietnam, research on Paragonimus and paragonimiasis has been conducted in northern and central regions of the country. Using a combination of morphological and molecular methods, 7 Paragonimus species, namely P. heterotremus, P. westermani, P. skrjabini, P. vietnamensis, P. proliferus, P. bangkokenis and P. harinasutai, have been identified in Vietnam. Of these, the first 3, P. heterotremus, P. westermani and P. skrjabini, are known to infect humans in other countries. However, in Vietnam, only P. heterotremus, found in some northern provinces, has been shown to infect humans. Even nowadays, local people in some northern provinces, such as Lai Chau and Yen Bai, are still suffering from P. heterotremus infection. In some provinces of central Vietnam, the prevalence and infection intensity of P. westermani metacercariae in freshwater crabs (the second intermediate hosts) are extremely high, but human cases have not been reported. Likewise, although P. skrjabini was found in Thanh Hoa Province, its pathogenicity to humans in Vietnam still remains uncertain. The results of molecular phylogenetic analyses of Vietnamese Paragonimus species provides new insights on the phylogeny and taxonomy of the genus Paragonimus. Comprehensive molecular epidemiological and geobiological studies on the genus in Vietnam and adjacent countries are needed to clarify the biodiversity and public health significance of the lung flukes.

Citations

Citations to this article as recorded by  Crossref logo
  • Paragonimiasis in Southeast Asia: A 60-Year Bibliometric Analysis (1963–2023)
    Harley M. Rodriguez, Jose Ma. M. Angeles
    Acta Parasitologica.2025;[Epub]     CrossRef
  • An overview of human helminthioses in Vietnam: Their prevention, control and lessons learnt
    Hung Manh Nguyen, Dung Trung Do, Stephen E. Greiman, Ha Van Nguyen, Hien Van Hoang, Toan Quoc Phan, Phuc Pham-Duc, Henry Madsen
    Acta Tropica.2023; 238: 106753.     CrossRef
  • Lung flukes of the genusParagonimus: ancient and re-emerging pathogens
    David Blair
    Parasitology.2022; 149(10): 1286.     CrossRef
  • General overview of the current status of human foodborne trematodiasis
    Jong-Yil Chai, Bong-Kwang Jung
    Parasitology.2022; 149(10): 1262.     CrossRef
  • Spectrum of pleuropulmonary paragonimiasis: An analysis of 685 cases diagnosed over 22 years
    Chun-Seob Ahn, Jong Wook Shin, Jeong-Geun Kim, Weon-Young Lee, Insug Kang, Jung-Gi Im, Yoon Kong
    Journal of Infection.2021; 82(1): 150.     CrossRef
  • Pleural lump after paragonimiasis treated by thoracoscopy: A case report
    Yue Xie, Ya-Rui Luo, Meng Chen, Yi-Min Xie, Chen-Yu Sun, Qiang Chen
    World Journal of Clinical Cases.2021; 9(3): 666.     CrossRef
  • Paragonimus and its hosts in China: An update
    Xiao-Juan Zhou, Qian Yang, Qi-Hong Tan, Li-Yanyang Zhang, Lin-Bo Shi, Jie-Xin Zou
    Acta Tropica.2021; 223: 106094.     CrossRef
  • Paragonimiasis (invasion by pulmonary flukes)
    S. B. Chuelov, A. L. Rossina
    CHILDREN INFECTIONS.2021; 20(3): 48.     CrossRef
  • Molecular identification of morphologically similar microcercous cercariae of two trematode families, Paragonimidae and Troglotrematidae, concurrently found in the same snail species of the subfamily Triculinae
    Pham Ngoc Doanh, Hoang Van Hien, Bui Thi Dung
    Folia Parasitologica.2020;[Epub]     CrossRef
  • Paragonimiasis: A missed diagnosis from Nepal
    Shreya Shrivastav, Anamika Jha
    Respiratory Medicine Case Reports.2020; 31: 101298.     CrossRef
  • Paragonimus and paragonimiasis in Asia: An update
    Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama
    Acta Tropica.2019; 199: 105074.     CrossRef
  • Simultaneous Paragonimus infection involving the breast and lung: A case report
    Moon Young Oh, Ajung Chu, Jeong Hwan Park, Jong Yoon Lee, Eun Youn Roh, Young Jun Chai, Ki-Tae Hwang
    World Journal of Clinical Cases.2019; 7(24): 4292.     CrossRef
  • Morphological and molecular characterization of Paragonimus caliensis Little, 1968 (Trematoda: Paragonimidae) from Medellin and Pichinde, Colombia
    Carolina Lenis, Alicia Galiano, Imelda Vélez, Iván Darío Vélez, Carlos Muskus, Antonio Marcilla
    Acta Tropica.2018; 183: 95.     CrossRef
  • First intermediate hosts of Paragonimus spp. in Vietnam and identification of intramolluscan stages of different Paragonimus species
    Pham Ngoc Doanh, Luu Anh Tu, Hoang Van Hien, Nguyen Van Duc, Yoichiro Horii, David Blair, Yukifumi Nawa
    Parasites & Vectors.2018;[Epub]     CrossRef
  • A Case of Pulmonary Paragonimiasis with Chronic Abdominal Pain and Erythematous Rash in a 6-year-old Girl
    Ju Young Kim, Min Kyu Park, Yong Ju Lee, Sun Huh, Ky Young Cho
    Pediatric Infection and Vaccine.2018; 25(1): 54.     CrossRef
  • Development of an immunodiagnosis method using recombinant PsCP for detection of Paragonimus skrjabini infection in human
    Shasha Yu, Xilin Zhang, Wenbi Chen, Hong Zheng, Guoping Ai, Nan Ye, Ying Wang
    Parasitology Research.2017; 116(1): 377.     CrossRef
  • Molecular identification of the trematode Paragonimus in faecal samples from the wild cat Prionailurus bengalensis in the Da Krong Nature Reserve, Vietnam
    P.N. Doanh, H.V. Hien, L.A. Tu, N. Nonaka, Y. Horii, Y. Nawa
    Journal of Helminthology.2016; 90(6): 658.     CrossRef
  • Molecular and morphological variation of Paragonimus westermani in Vietnam with records of new second intermediate crab hosts and a new locality in a northern province
    N. PHAM DOANH, A. LUU TU, T. DUNG BUI, T. HO LOAN, NARIAKI NONAKA, YOICHIRO HORII, DAVID BLAIR, YUKIFUMI NAWA
    Parasitology.2016; 143(12): 1639.     CrossRef
  • A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
    Xu Guang Qiu, Fukumi Nakamura-Uchiyama, Yukifumi Nawa, Makoto Itoh
    Tropical Medicine and Health.2016;[Epub]     CrossRef
  • 15,899 View
  • 219 Download
  • Crossref

Original Articles

Susceptibility of Laboratory Rodents to Trichinella papuae
Lakkhana Sadaow, Pewpan M. Intapan, Thidarut Boonmars, Nimit Morakote, Wanchai Maleewong
Korean J Parasitol 2013;51(6):629-632.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.629

Members of the genus Trichinella are small nematodes that can infect a wide range of animal hosts. However, their infectivity varies depending on the parasite and host species combination. In this study, we examined the susceptibility of 4 species of laboratory rodents, i.e., mice, rats, hamsters, and gerbils to Trichinella papuae, an emerging non-encapsulated Trichinella species. Trichinella spiralis and Trichinella pseudospiralis were also included in this study for comparison. Fifteen animals of each rodent species were infected orally with 100 muscle larvae of each Trichinella species. Intestinal worm burden was determined at day 6 and 10 post-inoculation (PI). The numbers of muscle larvae were examined at day 45 PI. The reproductive capacity index (RCI) of the 3 Trichinella species in different rodent hosts was determined. By day 6 PI, 33.2-69.6% of the inoculated larvae of the 3 Trichinella species became adult worms in the small intestines of the host animals. However, in rats, more than 96% of adult worms of all 3 Trichinella species were expelled from the gut by day 10 PI. In gerbils, only 4.8-18.1% of adult worms were expelled by day 10 PI. In accordance with the intestinal worm burden and the persistence of adults, the RCI was the highest in gerbils with values of 241.5±41.0 for T. papuae, 432.6±48 for T. pseudospiralis, and 528.6±20.6 for T. spiralis. Hamsters ranked second and mice ranked third in susceptibility in terms of the RCI, Rats yielded the lowest parasite RCI for all 3 Trichinella species. Gerbils may be an alternative laboratory animal for isolation and maintenance of Trichinella spp.

Citations

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  • Viability of Trichinella spiralis in traditional sour pork fermentation and its inactivation by microwave heating: Implications for zoonotic risk and food safety
    Atchara Artchayasawat, Benjamabhorn Pumhirunroj, Sukhonthip Khueangchiangkhwang, Thidarut Boonmars, Parichart Boueroy, Porntip Laummaunwai, Panaratana Rattanasuwan
    Veterinary World.2025; : 1660.     CrossRef
  • Real-time PCR versus traditional and Nano-based ELISA in early detection of murine trichinellosis
    S. M. Mohammad, L. A. Hegazy, R. S. Abdel Hady, M. A. Salama, S. K. Hammad, S. M. Ibrahim
    Journal of Helminthology.2023;[Epub]     CrossRef
  • A potential herbal therapeutic for trichinellosis
    Asmaa M. El-kady, Iman A. M. Abdel-Rahman, Eman Sayed, Majed H. Wakid, Hussah M. Alobaid, Khalil Mohamed, Eman Abdullah Alshehri, Hayam Elshazly, Wafa Abdullah I. Al-Megrin, Furhan Iqbal, Hatem A. Elshabrawy, Ashraf G. Timsah
    Frontiers in Veterinary Science.2022;[Epub]     CrossRef
  • Reprint of: Survival of Trichinella spiralis in cured meat products
    Annette Johne, Matthias Filter, Jennifer Gayda, Anja Buschulte, Niels Bandick, Karsten Nöckler, Anne Mayer-Scholl
    Veterinary Parasitology.2021; 297: 109544.     CrossRef
  • Human Outbreak of Trichinellosis Caused by Trichinella papuae Nematodes, Central Kampong Thom Province, Cambodia
    Yannick Caron, Sotharith Bory, Michel Pluot, Mary Nheb, Sarin Chan, Sang Houn Prum, Sun Bun Hong Lim, Mala Sim, Yi Sengdoeurn, Ly Sovann, Virak Khieu, Isabelle Vallée, Hélène Yera
    Emerging Infectious Diseases.2020; 26(8): 1759.     CrossRef
  • Survival of Trichinella spiralis in cured meat products
    Annette Johne, Matthias Filter, Jennifer Gayda, Anja Buschulte, Niels Bandick, Karsten Nöckler, Anne Mayer-Scholl
    Veterinary Parasitology.2020; 287: 109260.     CrossRef
  • Immuno-proteomic analysis of Trichinella spiralis, T. pseudospiralis, and T. papuae extracts recognized by human T. spiralis-infected sera
    Chalermchai Somboonpatarakun, Rutchanee Rodpai, Pewpan M. Intapan, Oranuch Sanpool, Lakkhana Sadaow, Chaisiri Wongkham, Tonkla Insawang, Thidarut Boonmars, Wanchai Maleewong
    Parasitology Research.2018; 117(1): 201.     CrossRef
  • Differential immune responses in mice infected with the tissue-dwelling nematode Trichinella zimbabwensis
    W.N. Onkoba, M.J. Chimbari, J.M. Kamau, S. Mukaratirwa
    Journal of Helminthology.2016; 90(5): 547.     CrossRef
  • 8,579 View
  • 72 Download
  • Crossref
Angiostrongylus cantonensis: Scanning Electron Microscopic Observations on the Cuticle of Moulting Larvae
Xin Zeng, Jie Wei, Juan Wang, Feng Wu, Feng Fung, Xiaoying Wu, Xi Sun, Huanqing Zheng, Zhiyue Lv, Zhongdao Wu
Korean J Parasitol 2013;51(6):633-636.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.633

Angiostrongylus cantonensis is a parasitic nematode that needs to develop in different hosts in different larval stages. Freshwater snails, such as Pomacea canaliculata, are the intermediate host, and rats are the definitive host. Periodic shedding of the cuticle (moulting) is an important biological process for the survival and development of the parasite in the intermediate and definitive hosts. However, there are few studies on the cuticle alterations between different stages of this parasite. In this study, we observed the ultrastructural appearance and changes of the cuticle of the 2nd/3rd stage larvae (L2/L3) and the 3rd/4th stage larvae (L3/L4) using a scanning electron microscope. We also first divided L2/L3 into late L2 and early L3. The late L2 lacked alae, but possessed a pull-chain-like fissure. Irregular alignment of spherical particles on the cuticle were noted compared to the L3. Alae appeared in the early L3. The old cuticle turned into a thin film-like structure which adhered to the new cuticle, and spherical particles were seen regularly arranged on the surface of this structure. Regular rectangular cavities were found on the surface of L3/L4. The caudal structure of L3/L4 was much larger than that of L3, but caudal inflation, such as seen in L4, was not observed. These results are the first to reveal the ultrastructural changes of the cuticle of A. cantonensis before and after moulting of L2/L3 and L3/L4.

Citations

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  • Water transmission potential of Angiostrongylus cantonensis: Larval viability and effectiveness of rainwater catchment sediment filters
    Kathleen Howe, Lisa Kaluna, Alicia Lozano, Bruce Torres Fischer, Yaeko Tagami, Robert McHugh, Susan Jarvi, Matty Knight
    PLOS ONE.2019; 14(4): e0209813.     CrossRef
  • The genetic basis of adaptive evolution in parasitic environment from the Angiostrongylus cantonensis genome
    Lian Xu, Meng Xu, Xi Sun, Junyang Xu, Xin Zeng, Dai Shan, Dongjuan Yuan, Ping He, Weiming He, Yulan Yang, Shiqi Luo, Jie Wei, Xiaoying Wu, Zhen Liu, Xiaomin Xu, Zhensheng Dong, Langui Song, Beibei Zhang, Zilong Yu, Lifu Wang, Chi Zhang, Xiaodong Fang, Qia
    PLOS Neglected Tropical Diseases.2019; 13(11): e0007846.     CrossRef
  • Molluscicidal activity and mechanism of toxicity of a novel salicylanilide ester derivative against Biomphalaria species
    Ping He, Weisi Wang, Benjamin Sanogo, Xin Zeng, Xi Sun, Zhiyue Lv, Dongjuan Yuan, Liping Duan, Zhongdao Wu
    Parasites & Vectors.2017;[Epub]     CrossRef
  • Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen
    JOEL BARRATT, DOUGLAS CHAN, INDY SANDARADURA, RICHARD MALIK, DEREK SPIELMAN, ROGAN LEE, DEBORAH MARRIOTT, JOHN HARKNESS, JOHN ELLIS, DAMIEN STARK
    Parasitology.2016; 143(9): 1087.     CrossRef
  • 9,388 View
  • 115 Download
  • Crossref
Modulation of Antibody Responses against Gnathostoma spinigerum in Mice Immunized with Crude Antigen Formulated in CpG Oligonucleotide and Montanide ISA720
Pewpan M. Intapan, Chakrit Hirunpetcharat, Churairat Kularbkaew, Wiboonchai Yutanawiboonchai, Penchom Janwan, Wanchai Maleewong
Korean J Parasitol 2013;51(6):637-644.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.637

This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.

Citations

Citations to this article as recorded by  Crossref logo
  • Evaluation of immunodiagnostic tests for human gnathostomiasis using different antigen preparations of Gnathostoma spinigerum larvae against IgE, IgM, IgG, IgG1‐4 and IgG1 patterns of post‐treated patients
    Issariya Ieamsuwan, Dorn Watthanakulpanich, Urai Chaisri, Poom Adisakwattana, Paron Dekumyoy
    Tropical Medicine & International Health.2021; 26(12): 1634.     CrossRef
  • 8,748 View
  • 86 Download
  • Crossref
Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P
Tongjit Thanchomnang, Pewpan M. Intapan, Chairat Tantrawatpan, Viraphong Lulitanond, Sudchit Chungpivat, Piyanan Taweethavonsawat, Worasak Kaewkong, Oranuch Sanpool, Penchom Janwan, Wej Choochote, Wanchai Maleewong
Korean J Parasitol 2013;51(6):645-650.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.645

A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.

Citations

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  • Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis
    Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski
    Pathogens.2024; 13(6): 447.     CrossRef
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    Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski
    Pathogens.2024; 13(11): 950.     CrossRef
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Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi by Real-Time PCR with High Resolution Melting Analysis
Amornmas Kongklieng, Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Viraphong Lulitanond, Pusadee Sri-Aroon, Yanin Limpanont, Wanchai Maleewong
Korean J Parasitol 2013;51(6):651-656.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.651

Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were 84.5±0.07℃ and 85.7±0.07℃, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts.

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Morphological and Molecular Characterization of Lymnaeid Snails and Their Potential Role in Transmission of Fasciola spp. in Vietnam
Bui Thi Dung, Pham Ngoc Doanh, Dang Tat The, Ho Thi Loan, Bertrand Losson, Yannick Caron
Korean J Parasitol 2013;51(6):657-662.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.657

Freshwater snails of the family Lymnaeidae play an important role in the transmission of fascioliasis worldwide. In Vietnam, 2 common lymnaeid species, Lymnaea swinhoei and Lymnaea viridis, can be recognized on the basis of morphology, and a third species, Lymnaea sp., is known to exist. Recent studies have raised controversy about their role in transmission of Fasciola spp. because of confusion in identification of the snail hosts. The aim of this study is, therefore, to clarify the identities of lymnaeid snails in Vietnam by a combination of morphological and molecular approaches. The molecular analyses using the second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA clearly showed that lymnaeids in Vietnam include 3 species, Austropeplea viridis (morphologically identified as L. viridis), Radix auricularia (morphologically identified as L. swinhoei) and Radix rubiginosa (morphologically identified as Lymnaea sp.). R. rubiginosa is a new record for Vietnam. Among them, only A. viridis was found to be infected with Fasciola spp. These results provide a new insight into lymnaeid snails in Vietnam. Identification of lymnaeid snails in Vietnam and their role in the liver fluke transmission should be further investigated.

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Zoonotic Echinostome Infections in Free-Grazing Ducks in Thailand
Weerachai Saijuntha, Kunyarat Duenngai, Chairat Tantrawatpan
Korean J Parasitol 2013;51(6):663-667.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.663

Free-grazing ducks play a major role in the rural economy of Eastern Asia in the form of egg and meat production. In Thailand, the geographical location, tropical climate conditions and wetland areas of the country are suitable for their husbandry. These environmental factors also favor growth, multiplication, development, survival, and spread of duck parasites. In this study, a total of 90 free-grazing ducks from northern, central, and northeastern regions of Thailand were examined for intestinal helminth parasites, with special emphasis on zoonotic echinostomes. Of these, 51 (56.7%) were infected by one or more species of zoonotic echinostomes, Echinostoma revolutum, Echinoparyphium recurvatum, and Hypoderaeum conoideum. Echinostomes found were identified using morphological criteria when possible. ITS2 sequences were used to identify juvenile and incomplete worms. The prevalence of infection was relatively high in each region, namely, north, central, and northeast region was 63.2%, 54.5%, and 55.3%, respectively. The intensity of infection ranged up to 49 worms/infected duck. Free-grazing ducks clearly play an important role in the life cycle maintenance, spread, and transmission of these medically important echinostomes in Thailand.

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  • Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand
    Sothorn Anucherngchai, Thanawan Tejangkura, Thapana Chontananarth
    Asian Pacific Journal of Tropical Biomedicine.2016; 6(6): 539.     CrossRef
  • Mitochondrial DNA sequences of 37 collar-spined echinostomes (Digenea: Echinostomatidae) in Thailand and Lao PDR reveals presence of two species: Echinostoma revolutum and E. miyagawai
    Mitsuru Nagataki, Chairat Tantrawatpan, Takeshi Agatsuma, Tetsuro Sugiura, Kunyarat Duenngai, Paiboon Sithithaworn, Ross H. Andrews, Trevor N. Petney, Weerachai Saijuntha
    Infection, Genetics and Evolution.2015; 35: 56.     CrossRef
  • 10,140 View
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Ultrastructure of Spermatogenesis in the Testis of Paragonimus heterotremus
Nongnut Uabundit, Pipatphong Kanla, Phongphithak Puthiwat, Channarong Arunyanart, Kowit Chaiciwamongkol, Wanchai Maleewong, Pewpan M. Intapan, Sitthichai Iamsaard, Wiphawi Hipkaeo
Korean J Parasitol 2013;51(6):669-676.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.669

Lung fluke, Paragonimus heterotremus, is a flatworm causing pulmonary paragonimiasis in cats, dogs, and humans in Southeast Asia. We examined the ultrastructure of the testis of adult P. heterotremus with special attention to spermatogenesis and spermiogenesis using scanning and transmission electron microscopy. The full sequence of spermatogenesis and spermiogenesis, from the capsular basal lamina to the luminal surface, was demonstrated. The sequence comprises spermatogonia, spermatocytes with obvious nuclear synaptonemal complexes, spermatids, and eventual spermatozoa. Moreover, full steps of spermatid differentiation were shown which consisted of 1) early stage, 2) differentiation stage representing the flagella, intercentriolar body, basal body, striated rootlets, and electron dense nucleus of thread-like lamellar configuration, and 3) growing spermatid flagella. Detailed ultrastructure of 2 different types of spermatozoa was also shown in this study.

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  • Molecular identification of the trematode P. ichunensis stat. n. from lungs of siberian tigers justified reappraisal of Paragonimus westermani species complex
    Anastasia N. Voronova, Konstantin S. Vainutis, Tatiana V. Tabakaeva, Mikhail V. Sapotsky, Nadezhda N. Kakareka, Yury G. Volkov, Irina V. Galkina, Mikhail Yu. Shchelkanov
    Journal of Parasitic Diseases.2022; 46(3): 744.     CrossRef
  • 10,763 View
  • 116 Download
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Molecular Variation in the Paragonimus heterotremus Complex in Thailand and Myanmar
Oranuch Sanpool, Pewpan M. Intapan, Tongjit Thanchomnang, Penchom Janwan, Yukifumi Nawa, David Blair, Wanchai Maleewong
Korean J Parasitol 2013;51(6):677-681.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.677

Paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. Of the 7 members of the genus known in Thailand until recently, only P. heterotremus has been confirmed as causing human disease. An 8th species, P. pseudoheterotremus, has recently been proposed from Thailand, and has been found in humans. Molecular data place this species as a sister species to P. heterotremus, and it is likely that P. pseudoheterotremus is not specifically distinct from P. heterotremus. In this study, we collected metacercariae of both nominal species (identification based on metacercarial morphology) from freshwater crabs from Phetchabun Province in northern Thailand, Saraburi Province in central Thailand, and Surat Thani Province in southern Thailand. In addition, we purchased freshwater crabs imported from Myanmar at Myawaddy Province, western Thailand, close to the Myanmar-Thailand border. The DNAs extracted from excysted metacercariae were PCR-amplified and sequenced for ITS2 and cox1 genes. The ITS2 sequences were nearly identical among all samples (99-100%). Phylogenies inferred from all available partial cox1 sequences contained several clusters. Sequences from Indian P. heterotremus formed a sister group to sequences from P. pseudoheterotremus-type metacercariae. Sequences of P. heterotremus from Thailand, Vietnam, and China formed a separate distinct clade. One metacercaria from Phitsanulok Province was distinct from all others. There is clearly considerable genetic variation in the P. heterotremus complex in Thailand and the form referred to as P. pseudoheterotremus is widely distributed in Thailand and the Thai-Myanmar border region.

Citations

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  • Considerable genetic diversity within Paragonimus heterotremus in Luang Prabang, northern Lao People's Democratic Republic
    Alongkorn Nonthapa, Rutchanee Rodpai, Tongjit Thanchomnang, Patcharaporn Boonroumkaew, Lakkhana Sadaow, David Blair, Pewpan M. Intapan, Wanchai Maleewong, Virasack Banouvong, Sakhone Laymanivong, Oranuch Sanpool
    Infection, Genetics and Evolution.2025; 128: 105718.     CrossRef
  • Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): genetic characteristics, polymorphism, and implications for intersuperfamilial phylogeny
    Khue Thi Nguyen, Huong Thi Thanh Doan, Linh Thi Khanh Pham, Do Thi Roan, Takeshi Agatsuma, Pham Ngoc Doanh, Thanh Hoa Le
    Parasitology Research.2024;[Epub]     CrossRef
  • Morphological and Molecular Characterization of Paragonimus skrjabini Complex from Yunnan, China: A Brief Report
    Qiu-Hong Shu, Shu-De Li, Ming Tian, Yong Meng, Shu-Mei-Qi He, Min Zhu, Miao-Miao Wang, Wen-Lin Wang
    Acta Parasitologica.2022; 67(1): 316.     CrossRef
  • The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes
    Abigail Hui En Chan, Naowarat Saralamba, Sompob Saralamba, Jiraporn Ruangsittichai, Urusa Thaenkham
    BMC Genomics.2022;[Epub]     CrossRef
  • General overview of the current status of human foodborne trematodiasis
    Jong-Yil Chai, Bong-Kwang Jung
    Parasitology.2022; 149(10): 1262.     CrossRef
  • Genetic differentiation of Southeast Asian Paragonimus Braun, 1899 (Digenea: Paragonimidae) and genetic variation in the Paragonimus heterotremus complex examined by nuclear DNA sequences
    Chairat Tantrawatpan, Sumonta Tapdara, Takeshi Agatsuma, Oranuch Sanpool, Pewpan M. Intapan, Wanchai Maleewong, Weerachai Saijuntha
    Infection, Genetics and Evolution.2021; 90: 104761.     CrossRef
  • An outbreak of Paragonimus westermani infection among Cambodian technical intern trainees in Japan, exhibiting various extrapulmonary lesions
    Yoshifumi Nakagawa, Yuki Ikematsu, Takayuki Nakanishi, Yuji Ogawa, Ryuichi Taen, Yasunori Nakashima, Hironao Okabe, Ayako Yoshida, Haruhiko Maruyama
    Parasitology International.2021; 81: 102279.     CrossRef
  • Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis
    Lakkhana Sadaow, Oranuch Sanpool, Hiroshi Yamasaki, Wanchai Maleewong, Pewpan M. Intapan
    Acta Tropica.2020; 203: 105325.     CrossRef
  • Paragonimus and paragonimiasis in Asia: An update
    Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama
    Acta Tropica.2019; 199: 105074.     CrossRef
  • Morphological and molecular characterization of Paragonimus caliensis Little, 1968 (Trematoda: Paragonimidae) from Medellin and Pichinde, Colombia
    Carolina Lenis, Alicia Galiano, Imelda Vélez, Iván Darío Vélez, Carlos Muskus, Antonio Marcilla
    Acta Tropica.2018; 183: 95.     CrossRef
  • Gene diversity and genetic variation in lung flukes (genusParagonimus)
    David Blair, Yukifumi Nawa, Makedonka Mitreva, Pham Ngoc Doanh
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 6.     CrossRef
  • A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region
    K. Choudhary, A. Kumar Verma, S. Swaroop, N. Agrawal
    Helminthologia.2015; 52(3): 167.     CrossRef
  • 11,373 View
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Paragonimus paishuihoensis Metacercariae in Freshwater Crabs, Potamon lipkei, in Vientiane Province, Lao PDR
Shigehisa Habe, Pham Ngoc Doanh, Shinichiro Yahiro, Nanthasane Vannavong, Hubert Barennes, Peter Odermatt, Gilles Dreyfuss, Yoichiro Horii, Yukifumi Nawa
Korean J Parasitol 2013;51(6):683-687.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.683

Among Paragonimus species, P. paishuihoensis is one of the most mysterious and poorly understood species. Metacercariae are characterized by having a unique dendritically branched excretory bladder. However, the morphology of the adult worm remains unknown. To date, metacercariae of this species have been reported only in China and Thailand. In this study, we first found P. paishuihoensis metacercariae in freshwater crabs, Potamon lipkei, in Hinheub District, Vientiane, Lao PDR, with a prevalence of 77.7% and the average intensity of 10.3 (range 1-28) metacercariae per crab. The molecular data based on ITS2 and CO1 markers indicated that P. paishuihoensis from Laos and Thailand were almost completely identical and were close to members of the Paragonimus bangkokensis/Paragonimus harinasutai complex. Attempts to infect experimental animals (cats, dogs, and rats) with P. paishuihoensis were unsuccessful, suggesting that these animals might be unsuitable definitive hosts for the species. Further studies are necessary to elucidate the taxonomic status and life cycle of P. paishuihoensis.

Citations

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  • Lung flukes of the genusParagonimus: ancient and re-emerging pathogens
    David Blair
    Parasitology.2022; 149(10): 1286.     CrossRef
  • Paragonimus and paragonimiasis in Asia: An update
    Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama
    Acta Tropica.2019; 199: 105074.     CrossRef
  • Morphological and molecular identification of a lung fluke, Paragonimus macrorchis (Trematoda, Paragonimidae), found in central Lao PDR and its molecular phylogenetic status in the genus Paragonimus
    Oranuch Sanpool, Pewpan Maleewong Intapan, Tongjit Thanchomnang, Penchom Janwan, Sakhone Laymanivong, Hiromu Sugiyama, Wanchai Maleewong
    Parasitology International.2015; 64(6): 513.     CrossRef
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Molecular Differentiation of Opisthorchis viverrini and Clonorchis sinensis Eggs by Multiplex Real-Time PCR with High Resolution Melting Analysis
Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Porntip Laummaunwai, Viraphong Lulitanond, Pham Ngoc Doanh, Wanchai Maleewong
Korean J Parasitol 2013;51(6):689-694.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.689

Opisthorchis viverrini and Clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in Asia. The standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. However, the method has low sensitivity, and eggs of O. viverrini and C. sinensis are difficult to distinguish from each other and from those of some other trematodes. Here, we report a multiplex real-time PCR coupled with high resolution melting (HRM) analysis for the differentiation of O. viverrini and C. sinensis eggs in fecal samples. Using 2 pairs of species-specific primers, DNA sequences from a portion of the mitochondrial NADH dehydrogenase subunit 2 (nad 2) gene, were amplified to generate 209 and 165 bp products for O. viverrini and C. sinensis, respectively. The distinct characteristics of HRM patterns were analyzed, and the melting temperatures peaked at 82.4±0.09℃ and 85.9±0.08℃ for O. viverrini and C. sinensis, respectively. This technique was able to detect as few as 1 egg of O. viverrini and 2 eggs of C. sinensis in a 150 mg fecal sample, which is equivalent to 7 and 14 eggs per gram of feces, respectively. The method is species-specific, rapid, simple, and does not require fluorescent probes or post-PCR processing for discrimination of eggs of the 2 species. It offers a new tool for differentiation and detection of Asian liver fluke infections in stool specimens.

Citations

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  • Multiparasitism in Spain in a Korean Visiting Friends and Relatives: Case Report and Review of Imported Cases of Clonorchis sinensis in Europe
    Paola Cociancic, Jacklyn Comas, J. Guillermo Esteban
    Clinical Case Reports.2025;[Epub]     CrossRef
  • Study of the population genetic structure of Opisthorchis-like eggs in northern Thailand using mitochondrial genes
    Picha Suwannahitatorn, Mathirut Mungthin, Ittisak Subrungruang, Lakhanawan Charoensuk, Nithikoon Aksorn, Saiwasan Buathong, Krystyna Cwiklinski
    PLOS Neglected Tropical Diseases.2024; 18(8): e0012445.     CrossRef
  • An Approach for Egg Parasite Classification Based on Ensemble Deep Learning
    Narut Butploy, Wanida Kanarkard, Pewpan M. Intapan, Oranuch Sanpool
    Journal of Advanced Computational Intelligence and Intelligent Informatics.2023; 27(6): 1113.     CrossRef
  • Fish and Food-Fatale: Food-borne Trematode Opisthorchis viverrini and Cholangiocarcinoma
    S. Tan, M. Machrumnizar
    Helminthologia.2023; 60(4): 287.     CrossRef
  • Are Melanoides tuberculata and Tarebia granifera (Gastropoda, Thiaridae), suitable first intermediate hosts of Clonorchis sinensis in Vietnam?
    Hung Manh Nguyen, Hien Hoang Van, Loan Thi Ho, Yulia V. Tatonova, Henry Madsen, Xiao-Nong Zhou
    PLOS Neglected Tropical Diseases.2021; 15(1): e0009093.     CrossRef
  • Clonorchis sinensis and clonorchiasis
    Byoung-Kuk Na, Jhang Ho Pak, Sung-Jong Hong
    Acta Tropica.2020; 203: 105309.     CrossRef
  • Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
    Pyo Yun Cho, Ji-Yun Lee, Tae Im Kim, Jin-Ho Song, Sung-Jong Hong, Won Gi Yoo, Takafumi Tsuboi, Kwon-Soo Ha, Jae-Wan Jung, Satoru Takeo, Eun-Taek Han, Banchob Sripa, Sung-Tae Hong, Jong-Yil Chai, Ho-Woo Nam, Jhang Ho Pak, Tong-Soo Kim, Krystyna Cwiklinski
    PLOS Neglected Tropical Diseases.2020; 14(12): e0008998.     CrossRef
  • Clonorchiasis sinensis detected by laparoscopic exploration of biliary tracts in two patients with obstructive jaundice
    Xialei Liu, Genglong Zhu, Chaonong Cai, Zhiyue Lv, Jian Li
    BMC Infectious Diseases.2019;[Epub]     CrossRef
  • Development of Conventional Multiplex PCR: A Rapid Technique for Simultaneous Detection of Soil-Transmitted Helminths
    Vivornpun Sanprasert, Ruthairat Kerdkaew, Siriporn Srirungruang, Sarit Charuchaibovorn, Kobpat Phadungsaksawasdi, Surang Nuchprayoon
    Pathogens.2019; 8(3): 152.     CrossRef
  • Performance evaluation of existing immunoassays for Clonorchis sinensis infection in China
    Hong-Mei Li, Men-Bao Qian, Yi-Chao Yang, Zhi-Hua Jiang, Kang Wei, Jia-Xu Chen, Jun-Hu Chen, Ying-Dan Chen, Xiao-Nong Zhou
    Parasites & Vectors.2018;[Epub]     CrossRef
  • Molecular characterization of the unique Mesostephanus appendiculatus (Trematoda: Cyathocotylidae) by small ribosomal RNA from Egypt
    Nasr M. El-Bahy, Eman K. Bazh, Shimaa S. Sorour, Nagwa M. Elhawary
    Parasitology Research.2017; 116(4): 1129.     CrossRef
  • LAMPhimerus: A novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples
    William Cevallos, Pedro Fernández-Soto, Manuel Calvopiña, Cristina Fontecha-Cuenca, Hiromu Sugiyama, Megumi Sato, Julio López Abán, Belén Vicente, Antonio Muro, David Blair
    PLOS Neglected Tropical Diseases.2017; 11(6): e0005672.     CrossRef
  • Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand
    Saiwasan Buathong, Saovanee Leelayoova, Mathirut Mungthin, Toon Ruang-areerate, Tawee Naaglor, Picha Suwannahitatorn, Phunlerd Piyaraj, Paanjit Taamasri, Peerapan Tan-ariya, Edoardo Pozio
    PLOS Neglected Tropical Diseases.2017; 11(11): e0006030.     CrossRef
  • Current status ofClonorchis sinensisand clonorchiasis in China
    De-Hua Lai, Xiao-Kun Hong, Bi-Xiu Su, Chi Liang, Geoff Hide, Xiaoli Zhang, Xinbing Yu, Zhao-Rong Lun
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 21.     CrossRef
  • 10,874 View
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Curcumin Prevents Bile Canalicular Alterations in the Liver of Hamsters Infected with Opisthorchis viverrini
Prapaporn Jattujan, Somchai Pinlaor, Lakhanawan Charoensuk, Channarong Arunyanart, Jariya Umka Welbat, Wunnee Chaijaroonkhanarak
Korean J Parasitol 2013;51(6):695-701.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.695

Opisthorchis viverrini infection causes inflammation and liver injury leading to periductal fibrosis. Little is known about the pathological alterations in bile canaliculi in opisthorchiasis. This study aimed to investigate bile canalicular alterations in O. viverrini-infected hamsters and to examine the chemopreventive effects of curcumin on such changes. Hamsters were infected with O. viverrini and one group of animals was fed with 1% dietary curcumin supplement. Animals were examined during the acute infection phase, days 21 and 30 post-infection (PI) and chronic infection phase (day 90 PI). Scanning electron microscopy revealed that in the infected group fed with a normal diet, bile canaliculi became slightly tortuous by 30 day PI and more tortuous at day 90 PI. Transmission electron microscopy showed a reduction in microvilli density of canaliculi starting at day 30 PI, with a marked loss of microvilli at day 90 PI. These ultrastructral changes were slightly seen at day 21 PI, which was similar to that found in infected animals fed with 1% curcumin-supplemented diet. Notably, curcumin treatment prevented the reduction of microvilli density, reduced the dilation of bile canaliculi, and decreased the tortuosity of the bile canaliculi relative to non-infected animals on a normal diet at days 30 and 90 PI. These results suggest that curcumin reduces alteration of bile canaliculi and may be a promising agent to prevent the onset of bile duct abnormalities induced by O. viverrini infection.

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  • Components from Curcuma longa (Turmeric) Against Hepatobiliary Diseases Based on Gut–Liver Axis: Pharmacotherapeutic Properties and Potential Clinical Applications
    Tianhui Gao, Shuyi Wang, Zongping Zhu, Liting Lin, Yirong Luo, Meigui Lu, Wan Liao
    The American Journal of Chinese Medicine.2024; 52(02): 387.     CrossRef
  • Dietary Polyphenols Alleviate Autoimmune Liver Disease by Mediating the Intestinal Microenvironment: Challenges and Hopes
    Zhen Cheng, Yuehua Wang, Bin Li
    Journal of Agricultural and Food Chemistry.2022; 70(35): 10708.     CrossRef
  • Generation of oxidative stress and induction of apoptotic like events in curcumin and thymoquinone treated adult Fasciola gigantica worms
    Abdur Rehman, Rizwan Ullah, Divya Gupta, M.A. Hannan Khan, Lubna Rehman, Mirza Ahmar Beg, Asad U. Khan, S.M.A. Abidi
    Experimental Parasitology.2020; 209: 107810.     CrossRef
  • Nanoencapsulated curcumin and praziquantel treatment reduces periductal fibrosis and attenuates bile canalicular abnormalities in Opisthorchis viverrini-infected hamsters
    Lakhanawan Charoensuk, Porntip Pinlaor, Supason Wanichwecharungruang, Kitti Intuyod, Kulthida Vaeteewoottacharn, Apisit Chaidee, Puangrat Yongvanit, Chawalit Pairojkul, Natthakitta Suwannateep, Somchai Pinlaor
    Nanomedicine: Nanotechnology, Biology and Medicine.2016; 12(1): 21.     CrossRef
  • Bile canalicular changes and defective bile secretion in Opisthorchis viverrini-infected hamsters
    Lakhanawan Charoensuk, Porntip Pinlaor, Umawadee Laothong, Puangrat Yongvanit, Chawalit Pairojkul, Yukifumi Nawa, Somchai Pinlaor
    Folia Parasitologica.2014; 61(6): 512.     CrossRef
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Distribution and Abundance of Opisthorchis viverrini Metacercariae in Cyprinid Fish in Northeastern Thailand
Somchai Pinlaor, Sudarat Onsurathum, Thidarut Boonmars, Porntip Pinlaor, Nuttanan Hongsrichan, Apisit Chaidee, Ornuma Haonon, Wutipong Limviroj, Smarn Tesana, Sasithorn Kaewkes, Paiboon Sithithaworn
Korean J Parasitol 2013;51(6):703-710.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.703

To increase public health awareness for prevention of opisthorchiasis caused by eating raw freshwater fish, the distribution and abundance of Opisthorchis viverrini metacercariae (OV MC) was investigated in freshwater fish obtained from 20 provinces in northeastern Thailand between April 2011 and February 2012. A cross-sectional survey was conducted on 12,890 fish consisting of 13 species randomly caught from 26 rivers, 10 dams, and 38 ponds/lakes. Fish, were collected in each of the rainy and winter seasons from each province. Fish were identified, counted, weighed, and digested using pepsin-HCl. Samples were examined for OV MC by a sedimentation method, and metacercariae were identified under a stereomicroscope. OV MC were found in 6 species of fish; i.e., Cyclocheilichthys armatus, Puntius orphoides, Hampala dispar, Henicorhynchus siamensis, Osteochilus hasselti, and Puntioplites proctozysron from localities in 13 provinces. Among the sites where OV MC-infected fish were found, 70.0% were dams, 23.7% were ponds/lakes, and 7.7% were rivers. The mean intensity of OV MC ranged from 0.01 to 6.5 cysts per fish (or 1.3-287.5 cysts per kg of fish). A high mean intensity of OV MC per fish (>3 cysts) was found in 5 provinces: Amnat Charoen (6.5 cysts), Nakhon Phanom (4.3), Mukdahan (4.1), Khon Kaen, (3.5) and Si Sa Ket (3.4). In conclusion, OV MC are prevalent in natural cyprinid fish, with the infection rate varying according to fish species and habitats.

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  • High-fat/high-fructose diet and Opisthorchis viverrini infection promote metabolic dysfunction-associated steatotic liver disease via inflammation, fibrogenesis, and metabolic dysfunction
    Lakhanawan Charoensuk, Phonpilas Thongpon, Chutima Sitthirach, Apisit Chaidee, Kitti Intuyod, Chawalit Pairojkul, Ei Htet Htet Khin, Chanakan Jantawong, Kanjana Thumanu, Porntip Pinlaor, Nuttanan Hongsrichan, Somchai Pinlaor
    Acta Tropica.2025; 261: 107491.     CrossRef
  • Development of a Dielectric Heating System for Selective Thermal Targeting of Liver Fluke Regions in Cirrhinus microlepis
    Supatinee Kornsing, Sirigiet Phunklang, Chanchai Thongsopa, Piyaporn Krachodnok, Nuchanart Santalunai, Samran Santalunai
    Applied Sciences.2025; 15(10): 5466.     CrossRef
  • Distribution of Opisthorchis viverrini metacercariae and cyprinoid fish abundance in Nong Han Wetland, Thailand
    Wiwat Kaensa, Lakhanawan Charoensuk, Chadon Nakmai, Somchai Pinlaor, Apiporn T. Suwannatrai, Umawadee Laothong, Suksanti Prakobwong
    Parasite Epidemiology and Control.2025; 30: e00447.     CrossRef
  • Unpacking the black box: I. Knowledge production and public health narratives on liver fluke infection and cholangiocarcinoma in Northeast Thailand
    Yee Ting Lee, Alan D. Ziegler
    South East Asia Research.2025; 33(2): 133.     CrossRef
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    The Journal of Infectious Diseases.2025;[Epub]     CrossRef
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    Yi-Chen Wang, Andrea Law, Jutamas Namsanor, Paiboon Sithithaworn, Narong Khuntikeo
    Annals of the American Association of Geographers.2024; 114(8): 1781.     CrossRef
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    Naiyana Senasri, Nattiya Chumnanka, Patcharawalai Sriyasak, Supannee Suwanpakdee, Sugunya Kumla, Kosit Sreeputhorn
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    International Journal for Parasitology.2024; 54(10): 497.     CrossRef
  • Integrated One-Health approach for prevention and control of Opisthorchis viverrini infection in rural Thailand: a 3-year study
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    Parasitology Research.2024;[Epub]     CrossRef
  • Spatial Predictive Modeling of Liver Fluke Opisthorchis viverrine (OV) Infection under the Mathematical Models in Hexagonal Symmetrical Shapes Using Machine Learning-Based Forest Classification Regression
    Benjamabhorn Pumhirunroj, Patiwat Littidej, Thidarut Boonmars, Atchara Artchayasawat, Narueset Prasertsri, Phusit Khamphilung, Satith Sangpradid, Nutchanat Buasri, Theeraya Uttha, Donald Slack
    Symmetry.2024; 16(8): 1067.     CrossRef
  • Transcriptome changes of liver fluke Opisthorchis viverrini in diabetic hamsters
    Apisit Chaidee, Naruechar Charoenram, Chatchawan Sengthong, Rungtiwa Dangtakot, Porntip Pinlaor, Thatsanapong Pongking, Somchai Pinlaor
    Parasite.2024; 31: 54.     CrossRef
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Establishment of an Allo-Transplantable Hamster Cholangiocarcinoma Cell Line and Its Application for In Vivo Screening of Anti-Cancer Drugs
Nattapong Puthdee, Kulthida Vaeteewoottacharn, Wunchana Seubwai, Orasa Wonkchalee, Worasak Kaewkong, Amornrat Juasook, Somchai Pinlaor, Chawalit Pairojkul, Chaisiri Wongkham, Seiji Okada, Thidarut Boonmars, Sopit Wongkham
Korean J Parasitol 2013;51(6):711-717.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.711

Opisthorchis viverrini (O. viverrini) is a well-known causative agent of cholangiocarcinoma (CCA) in humans. CCA is very resistant to chemotherapy and is frequently fatal. To understand the pathogenesis of CCA in humans, a rodent model was developed. However, the development of CCA in rodents is time-consuming and the xenograft-transplantation model of human CCA in immunodeficient mice is costly. Therefore, the establishment of an in vivo screening model for O. viverrini-associated CCA treatment was of interest. We developed a hamster CCA cell line, Ham-1, derived from the CCA tissue of O. viverrini-infected and N-nitrosodimethylamine-treated Syrian golden hamsters. Ham-1 has been maintained in Dulbecco's Modified Essential Medium supplemented with 10% fetal bovine serum for more than 30 subcultures. These cells are mostly diploid (2n=44) with some being polyploid. Tumorigenic properties of Ham-1 were demonstrated by allograft transplantation in hamsters. The transplanted tissues were highly proliferative and exhibited a glandular-like structure retaining a bile duct marker, cytokeratin 19. The usefulness of this for in vivo model was demonstrated by berberine treatment, a traditional medicine that is active against various cancers. Growth inhibitory effects of berberine, mainly by an induction of G1 cell cycle arrest, were observed in vitro and in vivo. In summary, we developed the allo-transplantable hamster CCA cell line, which can be used for chemotherapeutic drug testing in vitro and in vivo.

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Complete Mitochondrial Genome of Haplorchis taichui and Comparative Analysis with Other Trematodes
Dongmin Lee, Seongjun Choe, Hansol Park, Hyeong-Kyu Jeon, Jong-Yil Chai, Woon-Mok Sohn, Tai-Soon Yong, Duk-Young Min, Han-Jong Rim, Keeseon S. Eom
Korean J Parasitol 2013;51(6):719-726.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.719

Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.

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A Cross-Sectional Study on Intestinal Parasitic Infections in Rural Communities, Northeast Thailand
Sirintip Boonjaraspinyo, Thidarut Boonmars, Butsara Kaewsamut, Nuttapon Ekobol, Porntip Laummaunwai, Ratchadawan Aukkanimart, Nadchanan Wonkchalee, Amornrat Juasook, Pranee Sriraj
Korean J Parasitol 2013;51(6):727-734.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.727

Despite the existence of effective anthelmintics, parasitic infections remain a major public health problem in Southeast Asia, including Thailand. In rural communities, continuing infection is often reinforced by dietary habits that have a strong cultural basis and by poor personal hygiene and sanitation. This study presents a survey of the prevalence of intestinal parasitic infections among the people in rural Thailand. The community-based cross-sectional study was conducted in villages in Khon Kaen Province, northeastern Thailand, from March to August 2013. A total of 253 stool samples from 102 males and 140 females, aged 2-80 years, were prepared using formalin-ethyl acetate concentration methods and examined using light microscopy. Ninety-four individuals (37.2%) were infected with 1 or more parasite species. Presence of parasitic infection was significantly correlated with gender (P=0.001); nearly half of males in this survey (49.0%) were infected. Older people had a higher prevalence than younger members of the population. The most common parasite found was Opisthorchis viverrini (26.9%), followed by Strongyloides stercoralis (9.5%), Taenia spp. (1.6%), echinostomes (0.4%), and hookworms (0.4%). The prevalence of intestinal protozoa was Blastocystis hominis 1.6%, Entamoeba histolytica 0.8%, Entamoeba coli 0.8%, Balantidium coli 0.4%, Iodamoeba b?tschlii 0.4%, and Sarcocystis hominis 0.4%. Co-infections of various helminths and protozoa were present in 15.9% of the people. The present results show that the prevalence of parasitic infections in this region is still high. Proactive education about dietary habits, personal hygiene, and sanitation should be provided to the people in this community to reduce the prevalence of intestinal parasite infections. Moreover, development of policies and programs to control parasites is needed.

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Case Reports

Clinical Manifestations of Eosinophilic Meningitis Due to Infection with Angiostrongylus cantonensis in Children
Kittisak Sawanyawisuth, Jarin Chindaprasirt, Vichai Senthong, Panita Limpawattana, Narong Auvichayapat, Sompon Tassniyom, Verajit Chotmongkol, Wanchai Maleewong, Pewpan M. Intapan
Korean J Parasitol 2013;51(6):735-738.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.735

Eosinophilic meningitis, caused by the nematode Angiostrongylus cantonensis, is prevalent in northeastern Thailand, most commonly in adults. Data regarding clinical manifestations of this condition in children is limited and may be different those in adults. A chart review was done on 19 eosinophilic meningitis patients aged less than 15 years in Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Thailand. Clinical manifestations and outcomes were reported using descriptive statistics. All patients had presented with severe headache. Most patients were males, had fever, nausea or vomiting, stiffness of the neck, and a history of snail ingestion. Six patients had papilledema or cranial nerve palsies. It was shown that the clinical manifestations of eosinophilic meningitis due to A. cantonensis in children are different from those in adult patients. Fever, nausea, vomiting, hepatomegaly, neck stiffness, and cranial nerve palsies were all more common in children than in adults.

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    Clinical Infectious Diseases.2016; 63(11): 1475.     CrossRef
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Sparganosis Presenting as Cauda Equina Syndrome with Molecular Identification of the Parasite in Tissue Sections
Adhiratha Boonyasiri, Pornsuk Cheunsuchon, Prajak Srirabheebhat, Hiroshi Yamasaki, Wanchai Maleewong, Pewpan M. Intapan
Korean J Parasitol 2013;51(6):739-742.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.739

A 52-year-old woman presented with lower back pain, progressive symmetrical paraparesis with sensory impairment, and sphincter disturbance. Magnetic resonance imaging (MRI) of the whole spine revealed multiple intradural extramedullary serpiginous-mass lesions in the subarachnoid space continuously from the prepontine to the anterior part of the medulla oblongata levels, C7, T2-T8, and T12 vertebral levels distally until the end of the theca sac and filling-in the right S1 neural foramen. Sparganosis was diagnosed by demonstration of the sparganum in histopathological sections of surgically resected tissues and also by the presence of serum IgG antibodies by ELISA. DNA was extracted from unstained tissue sections, and a partial fragment of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was amplified using a primer set specific for Spirometra spp. cox1. After sequencing of the PCR-amplicon and alignment of the nucleotide sequence data, the causative agent was identified as the larva of Spirometra erinaceieuropaei.

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  • Sparganosis of the Cauda Equina: A Rare Case of Lower Thoracic Cord Edema and Diagnostic Challenges
    Prasert Iampreechakul, Chonlada Angsusing, Sunisa Hangsapruek, Samasuk Thammachantha, Adisak Tanpun
    Asian Journal of Neurosurgery.2025; 20(02): 378.     CrossRef
  • A case of cerebral sparganosis diagnosed by surgical resection and molecular analysis
    Ryo Miyahara, Osamu Akiyama, Naoko Yoshida, Mai Suzuki, Karin Ashizawa, Takuma Kodama, Yuzaburo Shimizu, Akihide Kondo
    Surgical Neurology International.2025; 16: 512.     CrossRef
  • Description of Spirometra asiana sp. nov. (Cestoda: Diphyllobothriidae) found in wild boars and hound dogs in Japan
    Hiroshi Yamasaki, Hiromu Sugiyama, Yasuyuki Morishima, Hirotaka Kobayashi
    Parasitology International.2024; 98: 102798.     CrossRef
  • Establishment of Animal Infection Model of Spirometra Mansoni and Identification of Spirometra Mansoni by Enzyme-Linked Immunosorbent Assay
    Anqi Luo, Shuyu Chen, Mingye He, Xiaoruo Tan, Zhikang Li, Wei Liu, Yisong Liu
    Vector-Borne and Zoonotic Diseases.2024;[Epub]     CrossRef
  • Sparganosis parasitic tumor: Resectability and postoperative outcome, and analysis of cases from an endemic area in Indochina
    Amnuay Kleebayoon, Viroj Wiwanitkit
    Formosan Journal of Surgery.2023; 56(5): 160.     CrossRef
  • Multiple sparganosis spinal infections mainly in the thoracic region: A case report
    Gan-Jun Wen, Jian Chen, Shi-Fei Zhang, Zhi-Sen Zhou, Gen-Long Jiao
    World Journal of Clinical Cases.2023; 11(36): 8507.     CrossRef
  • Morphological Characteristics and Molecular Phylogenetic Evidence Support the Existence of a New Spirometra Species, Spirometra Asiana, (Cestoda: Diphyllobothriidae) in the Wild Boar
    Hiroshi Yamasaki, Hiromu Sugiyama, Yasuyuki Morishima
    SSRN Electronic Journal .2022;[Epub]     CrossRef
  • Spirometra species from Asia: Genetic diversity and taxonomic challenges
    Hiroshi Yamasaki, Oranuch Sanpool, Rutchanee Rodpai, Lakkhana Sadaow, Porntip Laummaunwai, Mesa Un, Tongjit Thanchomnang, Sakhone Laymanivong, Win Pa Pa Aung, Pewpan M. Intapan, Wanchai Maleewong
    Parasitology International.2021; 80: 102181.     CrossRef
  • Case Report: Sparganosis of the Cauda Equina
    Verajit Chotmongkol, Warinthorn Phuttharak, Kritsakorn Jingjit, Nipon Chaisuriya, Oranuch Sanpool, Soramon Chaichan, Sittichai Khamsai
    The American Journal of Tropical Medicine and Hygiene.2021; 104(1): 298.     CrossRef
  • Low prevalence of spargana infection in farmed frogs in the Yangtze River Delta of China
    Xiaoli Zhang, Rongsheng Mi, Yehua Zhang, Shijie Zhang, Tao Sun, Haiyan Jia, Yan Huang, Haiyan Gong, Xiangan Han, Zhaoguo Chen
    Infection, Genetics and Evolution.2020; 85: 104466.     CrossRef
  • Cauda equina syndrome secondary to extramedullary spinal sparganosis
    Tzu-Hsieh Hsu, Tzu-Tsao Chung, Yi-Ani Chen, Among-Hsuan Chung
    Formosan Journal of Surgery.2020; 53(6): 230.     CrossRef
  • Differential Diagnosis of Human Sparganosis Using Multiplex PCR
    Hyeong-Kyu Jeon, Kyu-Heon Kim, Woon-Mok Sohn, Keeseon S. Eom
    The Korean Journal of Parasitology.2018; 56(3): 295.     CrossRef
  • Rapid identification of nine species of diphyllobothriidean tapeworms by pyrosequencing
    Tongjit Thanchomnang, Chairat Tantrawatpan, Pewpan M. Intapan, Oranuch Sanpool, Viraphong Lulitanond, Somjintana Tourtip, Hiroshi Yamasaki, Wanchai Maleewong
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    Quan Liu, Ming-Wei Li, Ze-Dong Wang, Guang-Hui Zhao, Xing-Quan Zhu
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    R. S. Petrigh, N. P. Scioscia, G. M. Denegri, M. H. Fugassa
    Helminthologia.2015; 52(4): 355.     CrossRef
  • Nine Human Sparganosis Cases in Thailand with Molecular Identification of Causative Parasite Species
    Wanchai Maleewong, Pewpan M. Intapan, Adhiratha Boonyasiri, Pornsuk Cheunsuchon, Hiroshi Yamasaki, Yupin Suputtamongkol, Oranuch Sanpool
    The American Journal of Tropical Medicine and Hygiene.2014; 91(2): 389.     CrossRef
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Brief Communications
Modified Formalin-Ether Concentration Technique for Diagnosis of Human Strongyloidiasis
Witthaya Anamnart, Pewpan M. Intapan, Wanchai Maleewong
Korean J Parasitol 2013;51(6):743-745.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.743

We compared the efficacy and applicability of a modified formalin-ether concentration technique (M-FECT) to the conventional FECT (C-FECT) and the agar plate culture (APC) method for the detection of Strongyloides stercoralis larvae. For this purpose, we used 600 human fecal specimens collected in an endemic area of southern Thailand. In the M-FECT, we used 2 layers of wire meshes, instead of gauze, to avoid the loss by absorption/adhesion of larvae to the gauze during filtration, and we reduced the exposure time of S. stercoralis larvae in stool samples to formalin. By such simple modifications, the efficacy of M-FECT has become comparable to APC and was much better than that of C-FECT for the diagnosis of strongyloidiasis.

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  • Gastrointestinal Parasitic Infections in Macaca fascicularis in Northeast Thailand: A One Health Perspective on Zoonotic Risks
    Teputid Kuasit, Manachai Yingklang, Penchom Janwan, Wanchai Maleewong, Weerachai Saijuntha, Siriporn Kuanamon, Tongjit Thanchomnang
    Animals.2025; 15(14): 2112.     CrossRef
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    Catherine A. Gordon, Jürg Utzinger, Stephen Muhi, Sören L. Becker, Jennifer Keiser, Virak Khieu, Darren J. Gray
    Nature Reviews Disease Primers.2024;[Epub]     CrossRef
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    International Journal for Parasitology.2024; 54(10): 497.     CrossRef
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    Apisit Chaidee, Naruechar Charoenram, Chatchawan Sengthong, Rungtiwa Dangtakot, Porntip Pinlaor, Thatsanapong Pongking, Somchai Pinlaor
    Parasite.2024; 31: 54.     CrossRef
  • Epidemiology of Strongyloides stercoralis and Opisthorchis viverrini infections in northern and northeastern Thailand: Insights from urine-ELISA surveys
    Wansiri Wiraphongthongchai, Paiboon Sithithaworn, Kavin Thinkhamrop, Kulwadee Suwannatrai, Kulthida Y. Kopolrat, Chanika Worasith, Apiporn T. Suwannatrai
    Parasitology Research.2024;[Epub]     CrossRef
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    Sengaloun Phonekeo, Sengchanh Kounnavong, Manithong Vonglokham, Latsamy Siengsounthone, Anousin Homsana, Sascha Gummin, Penelope Vounatsu, Prawat Nittiyanant, Suchin Worawichawong, Wichai Aekplakorn, Peter Odermatt, Somphou Sayasone
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    Veterinary World.2022; : 1208.     CrossRef
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    Pornphutthachat Sota, Sutas Suttiprapa, Sirikachorn Tangkawattana, Manop Sripa, David Blair, Banchob Sripa
    Parasitology.2022; 149(10): 1334.     CrossRef
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    Ivana Camacho-Alvarez, Elia M. Chavez-Mamani, Goyens Philippe, Jenny M. Luizaga-López, Mary Cruz Torrico, Laurent Gétaz, Frédérique Jacobs
    BMC Primary Care.2022;[Epub]     CrossRef
  • Improved agar plate culture conditions for diagnosis of Strongyloides stercoralis
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    Acta Tropica.2020; 203: 105291.     CrossRef
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    Isabel Fradejas, J M Herrero-Martínez, Manuel Lizasoaín, Esperanza Rodríguez de las Parras, Ana Pérez-Ayala
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2018;[Epub]     CrossRef
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  • Risk factors for Opisthorchis viverrini infection in Nakhon Phanom, Thailand, where the infection is highly endemic
    Songkran Nakbun, Pramote Thongkrajai, Choosak Nithikathkul
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    Wilawan PUMIDONMING, Doaa SALMAN, Dulyatad GRONSANG, Abdelbaset E. ABDELBASET, Khamphon SANGKAEO, Shin-ichiro KAWAZU, Makoto IGARASHI
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    D. Buonfrate, F. Formenti, F. Perandin, Z. Bisoffi
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Molecular Detection of Ancylostoma duodenale, Ancylostoma ceylanicum, and Necator americanus in Humans in Northeastern and Southern Thailand
Issarapong Phosuk, Pewpan M. Intapan, Tongjit Thanchomnang, Oranuch Sanpool, Penchom Janwan, Porntip Laummaunwai, Witthaya Aamnart, Nimit Morakote, Wanchai Maleewong
Korean J Parasitol 2013;51(6):747-749.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.747

The 2 principal species of hookworms infecting humans are Necator americanus and Ancylostoma duodenale. Case studies on zoonotic hookworm infections with Ancylostoma ceylanicum and/or Ancylostoma caninum are known mainly from Asian countries. Of these 2 zoonotic species, only A. ceylanicum can develop to adulthood in humans. In the present study, we report a molecular-based survey of human hookworm infections present in southern and northeastern Thailand. Thirty larval hookworm samples were obtained from fecal agar plate cultures of 10 patients in northeastren Thailand and 20 in southern Thailand. Partial ITS1, 5.8S, and ITS2 regions of the ribosomal DNA genes were amplified using PCR. The amplicons were sequenced, aligned, and compared with other hookworm sequences in GenBank database. The results showed that, in Thailand, N. americanus is more prevalent than Ancylostoma spp. and is found in both study areas. Sporadic cases of A. ceylanicum and A. duodenale infection were seen in northeastern Thailand.

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    Chuchard Punsawad, Nonthapan Phasuk, Suchirat Bunratsami, Kanjana Thongtup, Niramon Siripakonuaong, Somchok Nongnaul
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    Catherine Gordon, Johanna Kurscheid, Malcolm Jones, Darren Gray, Donald McManus
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    Prasit Na-Ek, Oranuch Sanpool, Jurairat Jongthawin, Witthaya Anamnart, Pewpan M. Intapan, Pennapa Chamavit, Wanchai Maleewong
    Parasitology Research.2016; 115(7): 2817.     CrossRef
  • Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand
    Wilawan PUMIDONMING, Doaa SALMAN, Dulyatad GRONSANG, Abdelbaset E. ABDELBASET, Khamphon SANGKAEO, Shin-ichiro KAWAZU, Makoto IGARASHI
    Journal of Veterinary Medical Science.2016; 78(12): 1779.     CrossRef
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A Recombinant Matrix Metalloproteinase Protein from Gnathostoma spinigerum for Serodiagnosis of Neurognathostomiasis
Penchom Janwan, Pewpan M. Intapan, Hiroshi Yamasaki, Porntip Laummaunwai, Kittisak Sawanyawisuth, Chaisiri Wongkham, Chatchai Tayapiwatana, Amnat Kitkhuandee, Viraphong Lulitanond, Yukifumi Nawa, Wanchai Maleewong
Korean J Parasitol 2013;51(6):751-754.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.751

Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components of molecular mass 21 kDa and 24 kDa in larval extracts of Gnathostoma spinigerum (Gs 21/24), has high sensitivity and specificity for diagnosis of neurognathostomiasis. However, only very small amounts of the Gs 21/24 antigens can be prepared from parasites harvested from natural or experimental animals. To overcome this problem, we recently produced a recombinant matrix metalloproteinase (rMMP) protein from G. spinigerum. In this study, we evaluated this rMMP alongside the Gs 21/24 antigens for serodiagnosis of human neurognathostomiasis. We studied sera from 40 patients from Srinagarind Hospital, Khon Kaen University, Thailand, with clinical criteria consistent with those of neurognathostomiasis, and sera from 30 healthy control adults from Thailand. All sera were tested for specific IgG antibodies against both G. spinigerum crude larval extract and rMMP protein using immunoblot analysis. The sensitivity and specificity for both antigenic preparations were all 100%. These results show that G. spinigerum rMMP protein can be used as an alternative diagnostic antigen, in place of larval extract, for serodiagnosis of neurognathostomiasis.

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  • Protein and antigen profiles of third-stage larvae of Gnathostoma spinigerum assessed with next-generation sequencing transcriptomic information
    Kathyleen Nogrado, Tipparat Thiangtrongjit, Poom Adisakwattana, Paron Dekumyoy, Sant Muangnoicharoen, Charin Thawornkuno, Onrapak Reamtong
    Scientific Reports.2022;[Epub]     CrossRef
  • Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis
    Penchom Janwan, Pewpan M. Intapan, Lakkhana Sadaow, Rutchanee Rodpai, Hiroshi Yamasaki, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Phuangphaka Sadee, Wanchai Maleewong
    Diagnostics.2021; 11(5): 862.     CrossRef
  • Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development
    Tipparat Thiangtrongjit, Kathyleen Nogrado, Thawatchai Ketboonlue, Preeyarat Malaitong, Poom Adisakwattana, Onrapak Reamtong
    Pathogens.2021; 10(9): 1080.     CrossRef
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    Sara Althubaiti, Şenay Kafkas, Marwa Abdelhakim, Robert Hoehndorf
    Journal of Biomedical Semantics.2020;[Epub]     CrossRef
  • Human gnathostomiasis: a neglected food-borne zoonosis
    Guo-Hua Liu, Miao-Miao Sun, Hany M. Elsheikha, Yi-Tian Fu, Hiromu Sugiyama, Katsuhiko Ando, Woon-Mok Sohn, Xing-Quan Zhu, Chaoqun Yao
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Surveillance and diagnosis of zoonotic foodborne parasites
    Reza Zolfaghari Emameh, Sami Purmonen, Antti Sukura, Seppo Parkkila
    Food Science & Nutrition.2018; 6(1): 3.     CrossRef
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Detection of Gnathostoma spinigerum Antibodies in Sera of Non-Traumatic Subarachnoid Hemorrhage Patients in Thailand
Amnat Kitkhuandee, Waranon Munkong, Kittisak Sawanyawisuth, Penchom Janwan, Wanchai Maleewong, Pewpan M. Intapan
Korean J Parasitol 2013;51(6):755-757.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.755

Gnathostoma spinigerum can cause subarachnoid hemorrhage (SAH). The detection of specific antibodies in serum against G. spinigerum antigen is helpful for diagnosis of neurognathostomiasis. There is limited data on the frequency of G. spinigerum infection in non-traumatic SAH. A series of patients diagnosed as non-traumatic SAH at the Srinagarind Hospital, Khon Kaen University, Thailand between January 2011 and January 2013 were studied. CT or MR imaging of the brain was used for diagnosis of SAH. Patients were categorized as aneurysmal subarachnoid hemorrhage (A-SAH) or non-aneurysmal subarachnoid hemorrhage (NA-SAH) according to the results of cerebral angiograms. The presence of specific antibodies in serum against 21- or 24-kDa G. spinigerum antigen was determined using the immunoblot technique. The detection rate of antibodies was compared between the 2 groups. Of the 118 non-traumatic SAH patients for whom cerebral angiogram and immunoblot data were available, 80 (67.8%) patients had A-SAH, whereas 38 (32.2%) had NA-SAH. Overall, 23.7% were positive for specific antibodies against 21- and/or 24-kDa G. spinigerum antigen. No significant differences were found in the positive rate of specific antibodies against G. spinigerum in both groups (P-value=0.350).

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Successful Treatment of Brugia pahangi in Naturally Infected Cats with Ivermectin
Piyanan Taweethavonsawat, Sudchit Chungpivat
Korean J Parasitol 2013;51(6):759-761.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.759

Lymphatic filariasis is a common parasitic disease of cats in tropical regions including Thailand. The
objective
of this study was to determine the efficacy of ivermectin against microfilariae of Brugia pahangi in naturally infected cats. Eight cats naturally infected with B. pahangi were divided into control (untreated) and treated groups. Cats in the latter group were given ivermectin injection at 400 ?g/kg weekly for 2 months. Microfilariae were counted every week until 48 weeks. Microfilaremia was significantly decreased in the treated group 4 weeks after starting the treatment and become zero at week 9 and afterwards. On the other hand, cats in the control group had high microfilaremia throughout the study. It was successful to treat and control B. pahangi infection in naturally infected cats using ivermectin.

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    Jerapas Thongpiya, Doonyapat Sa-nguanraksa, Norasate Samarnthai, Patsharaporn T. Sarasombath
    Parasitology International.2021; 80: 102203.     CrossRef
  • Comparison of efficacy of ivermectin and diethylcarbamazine against naturally infected Brugia malayi microfilaria in dogs
    Poojary Vineeta Sadarama, Deepa Chirayath, Usha Narayana Pillai, Bindu Lakshmanan
    Journal of Parasitic Diseases.2019; 43(4): 554.     CrossRef
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    Ladawan Khowawisetsut, Patsharaporn T. Sarasombath, Suwich Thammapalo, Sumart Loymek, Therayot Korbarsa, Hathai Nochote, Achinya Phuakrod, Wej Choochote, Sirichit Wongkamchai
    Veterinary Parasitology.2017; 245: 42.     CrossRef
  • 7,659 View
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Evaluation of IgG4 Subclass Antibody Detection by Peptide-Based ELISA for the Diagnosis of Human Paragonimiasis Heterotrema
Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Porntip Laummaunwai, Nimit Morakote, Yoon Kong, Wanchai Maleewong
Korean J Parasitol 2013;51(6):763-766.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.763

A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values of 100%, 94.6%, 96.2%, 100%, and 88.9%, respectively. Cross reactivity was frequently seen against the sera of fascioliasis (75%) and hookworm infections (50%). Since differential diagnosis between paragonimiasis and fascioliasis can be easily done by clinical presentation and fascioliasis serology, this cross reaction is not a serious problem. Sera from patients with other parasitoses (0-25%) rarely responded to this synthetic antigen. This synthetic peptide antigen seems to be useful for development of a standardized diagnostic system for paragonimiasis.

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  • An immunochromatographic test using whole blood for rapid diagnosis of human paragonimiasis and its diagnostic usefulness
    Patcharaporn Boonroumkaew, Lakkhana Sadaow, Penchom Janwan, Rutchanee Rodpai, Oranuch Sanpool, Tongjit Thanchomnang, Hiroshi Yamasaki, Pewpan M. Intapan, Wanchai Maleewong
    Food and Waterborne Parasitology.2024; 37: e00246.     CrossRef
  • Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis
    Lakkhana Sadaow, Oranuch Sanpool, Hiroshi Yamasaki, Wanchai Maleewong, Pewpan M. Intapan
    Acta Tropica.2020; 203: 105325.     CrossRef
  • Paragonimus and paragonimiasis in Asia: An update
    Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama
    Acta Tropica.2019; 199: 105074.     CrossRef
  • A Case of Pulmonary Paragonimiasis with Chronic Abdominal Pain and Erythematous Rash in a 6-year-old Girl
    Ju Young Kim, Min Kyu Park, Yong Ju Lee, Sun Huh, Ky Young Cho
    Pediatric Infection and Vaccine.2018; 25(1): 54.     CrossRef
  • A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
    Xu Guang Qiu, Fukumi Nakamura-Uchiyama, Yukifumi Nawa, Makoto Itoh
    Tropical Medicine and Health.2016;[Epub]     CrossRef
  • Expression characteristics and specific antibody reactivity of diverse cathepsin F members of Paragonimus westermani
    Chun-Seob Ahn, Byoung-Kuk Na, Dong-ll Chung, Jeong-Geun Kim, Jin-Taek Kim, Yoon Kong
    Parasitology International.2015; 64(1): 37.     CrossRef
  • North American paragonimiasis: epidemiology and diagnostic strategies
    Peter U Fischer, Gary J Weil
    Expert Review of Anti-infective Therapy.2015; 13(6): 779.     CrossRef
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High Prevalence of Haplorchiasis in Nan and Lampang Provinces, Thailand, Proven by Adult Worm Recovery from Suspected Opisthorchiasis Cases
Adulsak Wijit, Nimit Morakote, Jaewwaew Klinchid
Korean J Parasitol 2013;51(6):767-769.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.767

Opisthorchiasis, a risk factor for cholangiocarcinoma in humans, is of public health importance in Thailand. The Annual Surveillance Reports from Nan and Lampang Provinces, Thailand, for the year 2011 showed an opisthorchiasis prevalence of over 70% by recovery of eggs in the feces. This study investigated whether most cases are actually due to minute intestinal flukes (MIF) rather than Opisthorchis viverrini, as the eggs of both can hardly be differentiated by morphology. Fifty and 100 cases from residents in Nan and Lampang, respectively, had stools positive for eggs initially assumed to be those of O. viverrini. Each patient was given praziquantel at 40 mg/kg in a single dose. After 2 hr, 30-45 ml of the purgative magnesium sulfate was given, and stools were collected up to 4 times sequentially. The stools were examined for adult worms by simple sedimentation. It was found that 39 of 50 cases (78.0%) from Nan Province had Haplorchis taichui, with intensities ranging from 5 to 1,250 with an average of 62 worms/case. Taenia saginata (7 cases) and Enterobius vermicularis (1 case) were other helminths recovered as the co-infectants. In Lampang Province, H. taichui was recovered from 69 cases (69.0%). The number of flukes recovered ranged from 1 to 4,277, with an average of 326 worms/case. Four cases had Phaneropsolus bonnei, and 10 T. saginata as the co-infectants. Adult specimens of O. viverrini were not recovered from any stool. Clearly, MIF infection, especially haplorchiasis, is more common in northern Thailand. These findings should encourage the Public Health Office to employ more specific tools than Kato's method for surveillance of opisthorchiasis in Thailand.

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  • Study of the population genetic structure of Opisthorchis-like eggs in northern Thailand using mitochondrial genes
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    eLife.2021;[Epub]     CrossRef
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    Kornkamon Lertsuwan, Supathra Phoaubon, Nathapol Tasnawijitwong, Jomnarong Lertsuwan
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  • Genetic Differentiation of Opisthorchis-Like Eggs in Northern Thailand Using Stool Specimens Under National Strategic Plan to Control Liver Fluke Infection and Cholangiocarcinoma
    Saiwasan Buathong, Kateitsaray Phaiphilai, Toon Ruang-areerate, Naruemon Sitthichot, Thanyapit Thita, Mathirut Mungthin, Picha Suwannahitatorn
    The American Journal of Tropical Medicine and Hygiene.2020; 103(3): 1118.     CrossRef
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    PLOS Neglected Tropical Diseases.2017; 11(11): e0006030.     CrossRef
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