Parasites, Hosts and Diseases

"Doo-Hee Yun"

Original Article

Experimental Murine Fascioliasis Derives Early Immune Suppression with Increased Levels of TGF-β and IL-4: Joon-Yong Chung, Young-An Bae, Doo-Hee Yun, Hyun-Jong Yang, Yoon Kong; Korean J Parasitol 2012;50(4):301-308.
Published online November 26, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.4.301

Abstract
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In fascioliasis, T-helper 2 (Th2) responses predominate, while little is known regarding early immune phenomenon. We herein analyzed early immunophenotype changes of BALB/c, C57BL/6, and C3H/He mice experimentally infected with 5 Fasciola hepatica metacercariae. A remarkable expansion of CD19⁺ B cells was observed as early as week 1 post-infection while CD4⁺/CD8⁺ T cells were down-regulated. Accumulation of Mac1⁺ cells with time after infection correlated well with splenomegaly of all mice strains tested. The expression of tumor necrosis factor (TNF)-α mRNA in splenocytes significantly decreased while that of IL-4 up-regulated. IL-1β expression was down-modulated in BALB/c and C57BL/6 mice, but not in C3H/He. Serum levels of transforming growth factor (TGF)-β were considerably elevated in all mice during 3 weeks of infection period. These collective results suggest that experimental murine fascioliasis might derive immune suppression with elevated levels of TGF-β and IL-4 during the early stages of infection.

Citations

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Evaluation of Th1/Th2, regulatory cytokines and transcriptional factor FoxP3 in sheep immunized with a partially protective and non-protective vaccine and challenged with Fasciola hepatica
María Teresa Ruiz-Campillo, Isabel Lourdes Pacheco, Nieves Abril, María José Bautista, Álvaro Martínez-Moreno, Francisco Javier Martínez-Moreno, Leandro Buffoni, José Pérez, Verónica Molina-Hernández, Rafael Zafra
Veterinary Research.2024;[Epub] CrossRef
Fascioliasis: Image Findings, Diagnosis, and Treatment
Jae Seung Lee
Clinical Ultrasound.2024; 9(1): 18. CrossRef
Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development
Luis Miguel Flores-Velázquez, María Teresa Ruiz-Campillo, Guillem Herrera-Torres, Álvaro Martínez-Moreno, Francisco Javier Martínez-Moreno, Rafael Zafra, Leandro Buffoni, Pablo José Rufino-Moya, Verónica Molina-Hernández, José Pérez
Frontiers in Veterinary Science.2023;[Epub] CrossRef
The immunosuppression effects of deforolimus (ridaforolimus, AP23573) on allograft organ transplantation
Lumin Wang, Yanping Li, Dawei Yang, Jiazhao Fu, Bin Zhao, Yaguang Li, Yanrong Ye, Zhongquan Qi
Clinical and Translational Discovery.2023;[Epub] CrossRef
Immunization of Goats with Recombinant Protein 14-3-3 Isoform 2(rHcftt-2) Induced Moderate Protection against Haemonchus contortus Challenge
Yongqian Bu, Caiwen Jia, Xiaowei Tian, Kalibixiati Aimulajiang, Muhammad Ali Memon, Ruofeng Yan, Xiaokai Song, Lixin Xu, Xiangrui Li
Pathogens.2020; 9(1): 46. CrossRef
Characterization of dendritic cells and follicular dendritic cells in the hepatic lymph nodes and liver of sheep experimentally infected with Fasciola hepatica
María Teresa Ruiz-Campillo, Verónica Molina-Hernández, María José Bautista, Isabel L. Pacheco, Rafael Zafra, Leandro Buffoni, Francisco Javier Martínez-Moreno, Alvaro Martínez-Moreno, José Pérez
Veterinary Research.2020;[Epub] CrossRef
Helminth infection-induced carcinogenesis: spectrometric insights from the liver flukes, Opisthorchis and Fasciola
Maria João Gouveia, Maria Y. Pakharukova, Gabriel Rinaldi, Viatcheslav A. Mordvinov, Paul J. Brindley, Fátima Gärtner, Nuno Vale, Martin Michaelis
Experimental Results.2020;[Epub] CrossRef
Fasciola and fasciolosis in ruminants in Europe: Identifying research needs
N. J. Beesley, C. Caminade, J. Charlier, R. J. Flynn, J. E. Hodgkinson, A. Martinez-Moreno, M. Martinez-Valladares, J. Perez, L. Rinaldi, D. J. L. Williams
Transboundary and Emerging Diseases.2018; 65: 199. CrossRef
Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection
Isabel L. Pacheco, Nieves Abril, Rafael Zafra, Verónica Molina-Hernández, Noelia Morales-Prieto, María J. Bautista, María T. Ruiz-Campillo, Raúl Pérez-Caballero, Alvaro Martínez-Moreno, José Pérez
Veterinary Research.2018;[Epub] CrossRef
Association of Fasciola hepatica Infection with Liver Fibrosis, Cirrhosis, and Cancer: A Systematic Review
Claudia Machicado, Jorge D. Machicado, Vicente Maco, Angelica Terashima, Luis A. Marcos, Hector H Garcia
PLOS Neglected Tropical Diseases.2016; 10(9): e0004962. CrossRef

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Brief Communications

Differential expression of the 27 kDa cathepsin L-like cysteine protease in developmental stages of Spirometra erinacei: Yoon Kong, Doo-Hee Yun, Seung-Yull Cho, Woon-Mok Sohn, Young-Bae Chung, Shin-Yong Kang; Korean J Parasitol 2000;38(3):195-199.
Published online September 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.3.195

Abstract
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The 27 kDa cathepsin L-like cysteine protease of Spirometra erinacei plerocercoid is known to play an important function in tissue penetration, nutrient uptake and immune modulation in human sparganosis. In the present study, the expression of this enzyme was examined at different developmental stages of S. erinacei including immature egg, coracidium, plerocercoid in tadpole and rat, and adult. Proteolytic activity against carboxybenzoyl-phenylalanyl-arginyl-7-amino-4-methylcoumarin was detected in the extracts of coracidia and plerocercoid while no activity was observed in those of immature egg and adult. The specific activity in coracidial extracts was lower than that in the plerocercoid. Reverse transcription-polymerase chain reaction and Northern blot analysis demonstrated that the gene was expressed in the coracidium and plerocercoid but not in immature egg and adult. These results suggest that the 27 kDa cysteine protease is only expressed in the stages involving active migration of the parasite in the host tissue.

Citations

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Surgical treatment of a patient with live intracranial sparganosis for 17 years
Jialing Hu, Kaili Liao, Xiaojin Feng, Danling Jiang, Hailin Liu, Qingcui Zheng, Hai Qiu, Fuzhou Hua, Guohai Xu, Chunhua Xu
BMC Infectious Diseases.2022;[Epub] CrossRef
Follow-up study of high-dose praziquantel therapy for cerebral sparganosis
Peng Zhang, Yang Zou, Feng-Xia Yu, Zheng Wang, Han Lv, Xue-Huan Liu, He-Yu Ding, Ting-Ting Zhang, Peng-Fei Zhao, Hong-Xia Yin, Zheng-Han Yang, Zhen-Chang Wang, Siddhartha Mahanty
PLOS Neglected Tropical Diseases.2019; 13(1): e0007018. CrossRef
A cysteine protease from Spirometra erinaceieuropaei plerocercoid is a critical factor for host tissue invasion and migration
Daigo Tsubokawa, Takeshi Hatta, Hiroki Maeda, Fusako Mikami, Yukinobu Goso, Takeshi Nakamura, M. Abdul Alim, Naotoshi Tsuji
Acta Tropica.2017; 167: 99. CrossRef
Characterization of Spirometra erinaceieuropaei Plerocercoid Cysteine Protease and Potential Application for Serodiagnosis of Sparganosis
Li Na Liu, Zhong Quan Wang, Xi Zhang, Peng Jiang, Xin Qi, Ruo Dan Liu, Zi Fang Zhang, Jing Cui, Xiao-Nong Zhou
PLOS Neglected Tropical Diseases.2015; 9(6): e0003807. CrossRef
Diagnosis and stereotactic aspiration treatment of cerebral sparganosis: summary of 11 cases
Lei Deng, Pengju Xiong, Suokai Qian
Journal of Neurosurgery.2011; 114(5): 1421. CrossRef
Identification of host immune regulation candidate genes of Toxascaris leonina by expression sequenced tags (ESTs) analysis
Min Kyoung Cho, Keun Hee Lee, Sun Joo Lee, Se Won Kang, Mee Sun Ock, Yeon Chul Hong, Yong Seok Lee, Hak Sun Yu
Veterinary Parasitology.2009; 164(2-4): 242. CrossRef
Comparison of carbohydrate moieties of sparganum proteins of the snake, mouse and those of adult worm
Hyun Jong Yang
The Korean Journal of Parasitology.2003; 41(2): 135. CrossRef

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The 10 kDa protein of Taenia solium metacestodes shows genus specific antigenicity: Seung-Kyu Park, Doo-Hee Yun, Joon-Yong Chung, Yoon Kong, Seung-Yull Cho; Korean J Parasitol 2000;38(3):191-194.
Published online September 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.3.191

Abstract
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Genus specific antigenicity of the 10 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was demonstrated by comparative immunoblot analysis. When CFs from taeniid metacestodes of T. saginata, T. solium, T. taeniaeformis and T. crassiceps were probed with specific monoclonal antibody (mAb) raised against 150 kDa protein of T. solium metacestodes, specific antibody reactions were observed in 7 and 10 kDa proteins of T. solium and in 7/8 kDa of T. saginata, T. taeniaeformis and T. crassiceps. The mAb did not react with any protein in hydatid fluid of Echinococcus granulosus and E. multilocularis. This result revealed that the 10 kDa peptide of T. solium metacestodes and its equivalent proteins of different Taenia metacestodes are genus specific antigens that are shared among different Taenia species.

Citations

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Echinococcus granulosus antigen B: A Hydrophobic Ligand Binding Protein at the host–parasite interface
Valeria Silva-Álvarez, Ana Maite Folle, Ana Lía Ramos, Fernando Zamarreño, Marcelo D. Costabel, Eduardo García-Zepeda, Gustavo Salinas, Betina Córsico, Ana María Ferreira
Prostaglandins, Leukotrienes and Essential Fatty Acids.2015; 93: 17. CrossRef
Development of the S3Pvac Vaccine Against MurineTaenia crassicepsCysticercosis: A Historical Review
Edda Sciutto, Gladis Fragoso, Marisela Hernández, Gabriela Rosas, José J. Martínez, Agnès Fleury, Jacquelynne Cervantes, Aline Aluja, Carlos Larralde
Journal of Parasitology.2013; 99(4): 693. CrossRef

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70 Download
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Original Article

Persisting antibody reaction in paragonimiasis after praziquantel treatment is elicited mainly by egg antigens: Seung-Yull Cho, Yoon Kong, Doo-Hee Yun, Shin-Yong Kang, Lee-Soo Kim, Young-Bae Chung, Hyun-Jong Yang; Korean J Parasitol 2000;38(2):75-84.
Published online June 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.2.75

Abstract
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Antibody responses in serum and cerebrospinal fluid (CSF) samples from patients with active and chronic paragonimiasis and in sera from patients on whom follow-up studies were done after praziquantel treatment were analyzed using antigens of Paragonimus westermani prepared from eggs, metacercariae, juveniles of 4- and 7-week old, adult worms and recombinant protein of 28 kDa cruzipain-like cysteine protease (rPw28CCP). The patient sera/CSFs of active and chronic paragonimiasis revealed strong antibody reactions against the crude extracts of 4- and 7-week old juveniles as well as against those from egg and adult. rPw28CCP also showed specific reaction to the sera with active paragonimiasis. After the treatment, levels of specific antibodies in the sera gradually decreased to negative range in most patients. In some cases with persisting high antibody levels, however, the reactions at 27 kDa egg protein were sustained throughout the observation period of 34 months. The reactions at 35 and 32 kDa in adult extract and rPw28CCP disappeared rapidly after the treatment. Persistent antibody reactions even after successful treatment are provoked by continuous antigenic challenge from eggs which were not resolved by treatment.

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