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"Niger"

Brief Communication

Genetic polymorphism of merozoite surface protein 1 and antifolate-resistant genes in Plasmodium falciparum from Mali and Niger
Mahaman Moustapha Lamine, Rabia Maman, Abdoul Aziz Maiga, Ibrahim Maman Laminou
Parasites Hosts Dis 2023;61(4):455-462.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23049
Since 2015, countries in the Sahel region have implemented large-scale seasonal malaria chemoprevention (SMC). However, the mass use of sulfadoxine-pyrimethamine (SP) plus amodiaquine impacts the genetic diversity of malaria parasites and their sensitivity to antimalarials. This study aimed to describe and compare the genetic diversity and SP resistance of Plasmodium falciparum strains in Mali and Niger. We collected 400 blood samples in Mali and Niger from children aged 3–59 months suspected of malaria. Of them, 201 tested positive (Niger, 111, 55.2%; Mali, 90, 44.8%). Polymorphism of merozoite surface protein 1 (msp1) genetic marker showed 201 allotypes. The frequency of the RO33 allotype was significantly higher in Niger (63.6%) than in Mali (39.3%). There was no significant difference in the frequency of the K1 and MAD20 allotypes between the 2 countries. The multiplicity of infection was 2 allotypes per patient in Mali and one allotype per patient in Niger. The prevalence of strains with the triple mutants Pfdhfr51I/Pfdhfr59R/Pfdhps436A/F/H and Pfdhfr51I/Pfdhfr59R/Pfdhps437G was 18.1% and 30.2%, respectively, and 7.7% carried the quadruple mutant Pfdhfr51I/Pfdhfr59R/Pfdhps436A/F/H/Pfdhps437G. Despite the significant genetic diversity of parasite populations, the level of SP resistance was comparable between Mali and Niger. The frequency of mutations conferring resistance to SP still allows its effective use in intermittent preventive treatment in pregnant women and in SMC.

Citations

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  • Preventive interventions and diagnostic testing compliance in the management of tropical infections among patients with sickle cell disease in Tanzania
    Felician Paschal Mgasa, Singeun Oh, Avelina Mgasa, Sunjoo Kang, Erasto Mbugi, Ju Yeong Kim
    Parasites, Hosts and Diseases.2025; 63(2): 147.     CrossRef
  • Micro-geographic variation in antigenic diversity of PfEBA-175 region II in asymptomatic Plasmodium falciparum infections in Tanzania
    Jadidan Hada Syahada, Wang-Jong Lee, Hojong Jun, Johnsy Mary Louis, Fadhila Fitriana, Fauzi Muh, Feng Lu, Md Atique Ahmed, Sunghun Na, Wanjoo Chun, Won Sun Park, Bo-Young Jeon, Eun-Teak Han, Jim Todd, Alphaxard Manjurano, Winifrida Kidima, Ernest Mazigo,
    Frontiers in Immunology.2025;[Epub]     CrossRef
  • 2,342 View
  • 126 Download
  • 3 Web of Science
  • Crossref

Original Articles

Malaria Endemicity in the Rural Communities of Ebonyi State, Nigeria
David Ekene Nwele, Ikechukwu Oliver Onyali, Milliam Okwudili Iwueze, Michael Okpara Elom, Ogbonna Elom Sabastian Uguru
Korean J Parasitol 2022;60(3):173-179.
Published online June 30, 2022
DOI: https://doi.org/10.3347/kjp.2022.60.3.173
Malaria remains a global health threat. Approximately 97% of the population is at risk in sub-Saharan countries, particularly Nigeria. This study compared the performance of 2 diagnostic methods in assessing malaria endemicity in the rural communities of Ebonyi State, Nigeria. A total of 1,140 study participants were screened for malaria parasite using Rapid Diagnostic Test kits (RDT) in the field, while thick and thin films for microscopy were examined in the laboratory. Our result showed that malaria prevalence was 56.8 by RDT and 38.6% by microscopic test. Age group under 10 years had the highest prevalence of 28.9% (RDT) and 23.6% (microscopy), respectively. The highest prevalence of 19.5% by RDT was recorded in Onicha Local Government Area, while the highest prevalence of 13.4% with microscopy was recorded in Ezza North Local Government Area. The sensitivity and specificity of microscopic examination were both 100%, while those of RDT were 95.5% and 75.9%, respectively.

Citations

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  • Subclinical Plasmodium spp. Infections in a Community Setting in Bangui, Central African Republic
    Romaric Nzoumbou-Boko, Mireille Cornelia Ingrid Denissio Morissi Nalingbo, Brice Martial Yambiyo, Roger Detol, Ermeland Moussa, Didita Nalinga, Lydie Joella-Venus de la Grace Namsenei-Dankpea, Alexandre Manirakiza, Lawrence Ayong, Yap Boum
    Research and Reports in Tropical Medicine.2025; Volume 16: 1.     CrossRef
  • Explainable AI for enhanced accuracy in malaria diagnosis using ensemble machine learning models
    Olushina Olawale Awe, Peter Njoroge Mwangi, Samuel Kotva Goudoungou, Ruth Victoria Esho, Olanrewaju Samuel Oyejide
    BMC Medical Informatics and Decision Making.2025;[Epub]     CrossRef
  • Ectoparasite species diversity and prevalence in pigs (Sus scrofa domesticus) within delta central senatorial district, Delta State, Nigeria

    International Journal of Biosciences (IJB).2025; : 320.     CrossRef
  • Analysis of fractional-order model for the transmission dynamics of malaria via Caputo–Fabrizio and Atangana–Baleanu operators
    Benedict Celestine Agbata, Raimonda Dervishi, Mehmet Gümüş, Aseel Smerat, Godwin Christopher Ezike Mbah
    Scientific Reports.2025;[Epub]     CrossRef
  • Estimated distribution of malaria cases among children in sub-Saharan Africa by specified age categories using data from the Global Burden of Diseases 2019
    Olorunfemi A. Oshagbemi, Pedro Lopez-Romero, Cornelis Winnips, Katalin R. Csermak, Guoqin Su, Elodie Aubrun
    Malaria Journal.2023;[Epub]     CrossRef
  • 5,400 View
  • 171 Download
  • 4 Web of Science
  • Crossref
Larval Gnathostoma spinigerum Detected in Asian Swamp Eels, Monopterus albus, Purchased from a Local Market in Yangon, Myanmar
Jong-Yil Chai, Woon-Mok Sohn, Byoung-Kuk Na, Jong-Bok Park, Hoo-Gn Jeoung, Eui-Hyug Hoang, Thi Thi Htoon, Htay Htay Tin
Korean J Parasitol 2015;53(5):619-625.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.619
The present study was performed to determine the infection status of swamp eels with Gnathostoma sp. larvae in Myanmar. We purchased total 37 Asian swamp eels, Monopterus albus, from a local market in Yangon in June and December 2013 and 2014. All collected eels were transferred with ice to our laboratory and each of them was examined by the artificial digestion technique. A total of 401 larval gnathostomes (1-96 larvae/eel) were detected in 33 (89.2%) swamp eels. Most of the larvae (n=383; 95.5%) were found in the muscle. The remaining 18 larvae were detected in the viscera. The advanced third-stage larvae (AdL3) were 2.3-4.4 mm long and 0.25-0.425 mm wide. The characteristic head bulb (0.093 × 0.221 mm in average size) with 4 rows of hooklets, muscular long esophagus (1.025 mm), and 2 pairs of cervical sacs (0.574 mm) were observed by light microscopy. The average number of hooklets in the 1st, 2nd, 3rd, and 4th rows was 41, 45, 48, and 51, respectively. As scanning electron microscopic findings, the characteristic 4-5 rows of hooklets on the head bulb, a cervical papilla, tegumental spines regularly arranged in the transverse striations, and an anus were well observed. Based on these morphological characters, they were identified as the AdL3 of Gnathostoma spinigerum. By the present study, it has been confirmed for the first time that Asian swamp eels, M. albus, from Yangon, Myanmar are heavily infected with G. spinigerum larvae.

Citations

Citations to this article as recorded by  Crossref logo
  • Regulation of immune response against third-stage Gnathostoma spinigerum larvae by human genes
    Pattarasuda Puasri, Wilanee Dechkhajorn, Paron Dekumyoy, Tippayarat Yoonuan, Sumate Ampawong, Onrapak Reamtong, Usa Boonyuen, Surachet Benjathummarak, Yaowapa Maneerat
    Frontiers in Immunology.2023;[Epub]     CrossRef
  • Human gnathostomiasis: A review on the biology of the parasite with special reference on the current therapeutic management
    Kathyleen Nogrado, Poom Adisakwattana, Onrapak Reamtong
    Food and Waterborne Parasitology.2023; 33: e00207.     CrossRef
  • A comparative transcriptome analysis focusing on immune responses of Asian swamp eel following infection with Aeromonas hydrophila
    Dingda Xu, Panpan Han, Lihai Xia, Jinping Gan, Qiaoqing Xu
    Aquaculture.2021; 539: 736655.     CrossRef
  • Detection of Gnathostoma spinigerum Advanced 3rd-Stage Larvae in the Chinese Edible Frog, Hoplobatrachus rugulosus, from Local Markets in Phnom Penh, Cambodia
    Woon-Mok Sohn, Bong-Kwang Jung, Sooji Hong, Seungwan Ryoo, Keon Hoon Lee, Virak Khieu, Jong-Yil Chai
    The Korean Journal of Parasitology.2021; 59(5): 519.     CrossRef
  • Human gnathostomiasis: a neglected food-borne zoonosis
    Guo-Hua Liu, Miao-Miao Sun, Hany M. Elsheikha, Yi-Tian Fu, Hiromu Sugiyama, Katsuhiko Ando, Woon-Mok Sohn, Xing-Quan Zhu, Chaoqun Yao
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Effects of Gnathostoma spinigerum infective stage larva excretory-secretory products on NK cells in peripheral blood mononuclear cell culture: focused on expressions of IFN-γ and killer cell lectin-like receptors
    Thanyathon Khetsuphan, Urai Chaisri, Wilanee Dechkhajorn, Surachet Benjathummarak, Paron Dekumyoy, Sumate Ampawong, Parnpen Viriyavejakul, Yaowapa Maneerat
    Parasitology Research.2020; 119(3): 1011.     CrossRef
  • Larval Gnathostomes and Spargana in Chinese Edible Frogs, Hoplobatrachus rugulosus, from Myanmar: Potential Risk of Human Infection
    Jong-Yil Chai, Bong-Kwang Jung, Jin-Youp Ryu, Hyun-Seung Kim, Sung-Jong Hong, Thi Thi Htoon, Htay Htay Tin, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2020; 58(4): 467.     CrossRef
  • Larval Gnathostomes and Zoonotic Trematode Metacercariae in Fish from a Local Market in Yangon City, Myanmar
    Jong-Yil Chai, Bong-Kwang Jung, Keon Hoon Lee, Jin-Youp Ryu, Hyeon-Seung Kim, Sung-Jong Hong, Thi Thi Htoon, Htay Htay Tin, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2020; 58(6): 701.     CrossRef
  • Infection Status of Gnathostoma spinigerum Larvae in Asian Swamp Eels, Monopterus albus, Purchased from Local Markets in Cambodia
    Jong-Yil Chai, Bong-Kwang Jung, Keon Hoon Lee, Sung-Jong Hong, Virak Khieu, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2020; 58(6): 695.     CrossRef
  • Isolation, identification, and classification of a novel rhabdovirus from diseased Chinese rice-field eels (Monopterus albus)
    Wenzhi Liu, Yuding Fan, Zhong Li, Jianqing Zhao, Yong Zhou, Nan Jiang, Jia Zeng, Kenneth Cain, Lingbing Zeng
    Archives of Virology.2019; 164(1): 105.     CrossRef
  • Molecular identification and genetic diversity of Gnathostoma spinigerum larvae in freshwater fishes in southern Lao PDR, Cambodia, and Myanmar
    Patcharaporn Boonroumkaew, Oranuch Sanpool, Rutchanee Rodpai, Lakkhana Sadaow, Chalermchai Somboonpatarakun, Sakhone Laymanivong, Win Pa Pa Aung, Mesa Un, Porntip Laummaunwai, Pewpan M. Intapan, Wanchai Maleewong
    Parasitology Research.2019; 118(5): 1465.     CrossRef
  • Transcriptome and excretory–secretory proteome of infective-stage larvae of the nematode Gnathostoma spinigerum reveal potential immunodiagnostic targets for development
    Supaporn Nuamtanong, Onrapak Reamtong, Orawan Phuphisut, Palang Chotsiri, Preeyarat Malaithong, Paron Dekumyoy, Poom Adisakwattana
    Parasite.2019; 26: 34.     CrossRef
  • Aeromonas veronii caused disease and pathological changes in Asian swamp eel Monopterus albus
    Lihai Xia, Panpan Han, Xiaoli Cheng, Youshen Li, Chuwen Zheng, Hanwen Yuan, Wenbing Zhang, Qiaoqing Xu
    Aquaculture Research.2019; 50(10): 2978.     CrossRef
  • Gnathostomatidae nematode parasite of Colomesus psittacus (Osteichthyes, Tetraodontiformes) in the Ilha de Marajó, Brazilian Amazon
    Raul Henrique da Silva Pinheiro, Ricardo Luís Sousa Santana, Francisco Tiago Vasconcelos Melo, Jeannie Nascimento dos Santos, Elane Guerreiro Giese
    Revista Brasileira de Parasitologia Veterinária.2017; 26(3): 340.     CrossRef
  • 13,614 View
  • 141 Download
  • 15 Web of Science
  • Crossref

Brief Communication

High Genetic Variability of Schistosoma haematobium in Mali and Nigeria
Charles Ezeh, Mingbo Yin, Hongyan Li, Ting Zhang, Bin Xu, Moussa Sacko, Zheng Feng, Wei Hu
Korean J Parasitol 2015;53(1):129-134.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.129

Schistosoma haematobium is one of the most prevalent parasitic flatworms, infecting over 112 million people in Africa. However, little is known about the genetic diversity of natural S. haematobium populations from the human host because of the inaccessible location of adult worms in the host. We used 4 microsatellite loci to genotype individually pooled S. haematobium eggs directly from each patient sampled at 4 endemic locations in Africa. We found that the average allele number of individuals from Mali was significantly higher than that from Nigeria. In addition, no significant difference in allelic composition was detected among the populations within Nigeria; however, the allelic composition was significantly different between Mali and Nigeria populations. This study demonstrated a high level of genetic variability of S. haematobium in the populations from Mali and Nigeria, the 2 major African endemic countries, suggesting that geographical population differentiation may occur in the regions.

Citations

Citations to this article as recorded by  Crossref logo
  • Evidence of high genetic diversity among parasite populations in a schistosomiasis hotspot
    Yvonne Aryeetey Ashong, Emmanuel Odartei Armah, Jewelna Akorli, Frank Twum Aboagye, Isaac Owusu-Frimpong, Linda Batsa Debrah, Rhoda Lims Diyie, Samuel Armoo, Alexander Yaw Debrah, Mike Yaw Osei-Atweneboana, Clinton J. Jones, Marcello Otake Sato
    Microbiology Spectrum.2025;[Epub]     CrossRef
  • Geographical Influence on Morphometric Variability of Genetically “Pure” Schistosoma haematobium Eggs from Sub-Saharan Migrants in Spain
    Marta Reguera-Gómez, Maria Adela Valero, Patricio Artigas, Alejandra De Elías-Escribano, Maria Cecilia Fantozzi, Maria Pilar Luzón-García, Joaquín Salas-Coronas, Jérôme Boissier, Santiago Mas-Coma, Maria Dolores Bargues
    Tropical Medicine and Infectious Disease.2023; 8(3): 144.     CrossRef
  • Genetic Diversity of Schistosoma haematobium in Sub-Saharan Africa: A Systematic Review
    Rabecca Tembo, Panji Nkhoma, Mildred Zulu, Florence Mwaba, John Yabe, Hikabasa Halwiindi, Moses Kamwela, King S Nalubamba, Chummy S Sikasunge, Andrew M Phri
    University of Zambia Journal of Agricultural and Biomedical Sciences.2022;[Epub]     CrossRef
  • Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases
    Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon
    Clinical Microbiology Reviews.2021;[Epub]     CrossRef
  • Detection of Schistosoma DNA in genital specimens and urine: A comparison between five female African study populations originating from S. haematobium and/or S. mansoni endemic areas
    P. Pillay, J.A. Downs, J.M. Changalucha, E.A.T. Brienen, C.E. Ramarokoto, P.D.C. Leutscher, B.J. Vennervald, M. Taylor, E.F. Kjetland, L. Van Lieshout
    Acta Tropica.2020; 204: 105363.     CrossRef
  • RETRACTED: Diversity and Compatibility of Human Schistosomes and Their Intermediate Snail Hosts
    Benjamin Sanogo, Dongjuan Yuan, Xin Zeng, Yanhua Zhang, Zhongdao Wu
    Trends in Parasitology.2018; 34(6): 493.     CrossRef
  • Geographic strain differentiation of Schistosoma japonicum in the Philippines using microsatellite markers
    Kharleezelle J. Moendeg, Jose Ma M. Angeles, Ryo Nakao, Lydia R. Leonardo, Ian Kendrich C. Fontanilla, Yasuyuki Goto, Masashi Kirinoki, Elena A. Villacorte, Pilarita T. Rivera, Noboru Inoue, Yuichi Chigusa, Shin-ichiro Kawazu, Aaron R. Jex
    PLOS Neglected Tropical Diseases.2017; 11(7): e0005749.     CrossRef
  • Genetic Diversity of <i>Schistosoma haematobium</i> Eggs Isolated from Human Urine in Sudan
    Juan-Hua Quan, In-Wook Choi, Hassan Ahmed Hassan Ahmed Ismail, Abdoelohab Saed Mohamed, Hoo-Gn Jeong, Jin-Su Lee, Sung-Tae Hong, Tai-Soon Yong, Guang-Ho Cha, Young-Ha Lee
    The Korean Journal of Parasitology.2015; 53(3): 271.     CrossRef
  • 11,732 View
  • 125 Download
  • 9 Web of Science
  • Crossref

Original Article

Comparative Analysis of Pathogenic Organisms in Cockroaches from Different Community Settings in Edo State, Nigeria
Clement Isaac, Philip Ogbeide Orue, Mercy Itohan Iyamu, Joy Imuetiyan Ehiaghe, Osesojie Isaac
Korean J Parasitol 2014;52(2):177-181.
Published online April 18, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.2.177

Cockroaches are abundant in Nigeria and are seen to harbour an array of pathogens. Environmental and sanitary conditions associated with demographic/socio-economic settings of an area could contribute to the prevalence of disease pathogens in cockroaches. A total of 246 cockroaches (Periplaneta americana) in urban (Benin, n=91), semi-urban (Ekpoma, n=75) and rural (Emuhi, n=70) settings in Edo State, Nigeria were collected within and around households. The external body surfaces and alimentary canal of these cockroaches were screened for bacterial, fungal, and parasitological infections. Bacillus sp. and Escherichia coli were the most common bacteria in cockroaches. However, Enterococcus faecalis could not be isolated in cockroaches trapped from Ekpoma and Emuhi. Aspergillus niger was the most prevalent fungus in Benin and Ekpoma, while Mucor sp. was predominant in Emuhi. Parasitological investigations revealed the preponderance of Ascaris lumbricoides in Benin and Emuhi, while Trichuris trichura was the most predominant in Ekpoma. The prevalence and burden of infection in cockroaches is likely to be a reflection of the sanitary conditions of these areas. Also, cockroaches in these areas making incursions in homes may increase the risk of human infections with these disease agents.

Citations

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  • Cockroach Microbiome Disrupts Indoor Environmental Microbial Ecology with Potential Public Health Implications
    Jiahui Ma, Mengzhen Wang, Ye Sun, Yunhao Zheng, Senchao Lai, Yingyi Zhang, Yan Wu, Chao Jiang, Fangxia Shen
    Environment & Health.2025; 3(4): 380.     CrossRef
  • SURVEY OF MEDICALLY IMPORTANT PROTOZOANS INFESTED ON COCKROACHES COLLECTED FROM STUDENT HOSTEL IN SA’ADU ZUNGUR UNIVERSITY, BAUCHI STATE
    Abdullahi Kamal, Aliyu Abdulhamid Omar, Abdullahi Shamsudden , Ladan Muhammad Auwal, Umar Aminu Mohammed, Abubakar Sadiq Yusuf, Umar Aliyu, Firdausi Zakari Ya’u
    FUDMA JOURNAL OF SCIENCES.2025; 9(2): 61.     CrossRef
  • The Medical Importance of Cockroaches as Vectors of Pathogens: Implications for Public Health
    Dongfen Geng, Haotian Yu, Teng Zhao, Chunxiao Li
    Zoonoses.2025;[Epub]     CrossRef
  • Comparison of the Effectiveness of Eucalyptus camaldulensis Extract and Dichlorvos (DDVP) in Controlling the Periplaneta americana
    A H Alwan, S M Lafta, E K Athab
    IOP Conference Series: Earth and Environmental Science.2025; 1538(1): 012076.     CrossRef
  • Urban Cockroaches as Reservoirs of Multidrug-Resistant Bacteria: Insights from Morphological and Molecular Characterization
    Jaweria Riaz, Mubashar Hussain, Aniza Iftikhar, Kiran Aftab, Suleman Hussain Shah, Sheeza Sakhawat, Amina Zafar, Aqsa Noreen
    Revista Brasileira de Entomologia.2025;[Epub]     CrossRef
  • Intestinal Parasites Associated with American Cockroach (Periplaneta americana) in Akure, Ondo State, Nigeria
    Adebayo Victor Akeju, Titus Adeniyi Olusi, Regina-Tyra Ekwi Obi
    Journal of Parasitic Diseases.2024; 48(1): 67.     CrossRef
  • Extended spectrum and metalo beta lactamase producing gram negative bacterial pathogens from cockroaches collected at hospital, Southern Ethiopia
    Fithamlak Solomon Bisetegn, Habtamu Azene, Khawaja Shakeel Ahmed, Fiseha Wadilo, Efrata Girma Tufa
    Antimicrobial Resistance & Infection Control.2024;[Epub]     CrossRef
  • De Novo Assembly and Characterization of the Transcriptome of an Omnivorous Camel Cricket (Tachycines meditationis)
    Jun-Hui Lu, De-Long Guan, Sheng-Quan Xu, Huateng Huang
    International Journal of Molecular Sciences.2023; 24(4): 4005.     CrossRef
  • Species composition and parasite carriage rate of cockroaches among households of Sekota town, Northeast Ethiopia
    Habtu Debash, Megbaru Alemu, Animen Ayehu
    International Journal of Tropical Insect Science.2022; 42(6): 3815.     CrossRef
  • A Systematic Review of Zoonotic Enteric Parasites Carried by Flies, Cockroaches, and Dung Beetles
    Avi Patel, Meg Jenkins, Kelly Rhoden, Amber N. Barnes
    Pathogens.2022; 11(1): 90.     CrossRef
  • Experimental studies to study the efficacy of "Tarzan, VE" against Blattoptera representatives
    R. M. Akbaev, L. V. Nacheva, A. A. Generalov
    Russian Journal of Parasitology.2021; 15(2): 80.     CrossRef
  • Protozoan Cysts in Faecal Pellets of German Cockroaches (Blattella germanica), with Particular Emphasis on Lophomonas blattarum
    Hugo Cornelis van Woerden, Rafael Martínez-Girón, Cristina Martínez-Torre
    Acta Parasitologica.2020; 65(4): 831.     CrossRef
  • Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches
    Zohreh Abdolmaleki, Zohreh Mashak, Farhad Safarpoor Dehkordi
    Antimicrobial Resistance & Infection Control.2019;[Epub]     CrossRef
  • Spatial Distribution and Prevalence of Parasites Vectored by Periplaneta americana in Southern, Nigeria: Implication for Intervention
    Ito Edore Edwin
    Asian Journal of Biological Sciences.2019; 12(2): 313.     CrossRef
  • Contamination of Cockroaches (Insecta: Blattaria) by Medically Important Bacteriae: A Systematic Review and Meta-analysis
    Hassan Nasirian, Michael Rust
    Journal of Medical Entomology.2019; 56(6): 1534.     CrossRef
  • Identification of Cockroaches as Mechanical Vector for Parasitic Infections and Infestations in Kuantan, Malaysia
    Afzan Mat Yusof
    Journal of Entomology.2018; 15(3): 143.     CrossRef
  • Isolation of bacteria from the digestive tract of Periplaneta americana and Polyphaga aegyptiaca (Blattodea: Blattidae) in Khuzestan Province, Southwestern Iran
    Arezo Elyasigomari, Davood Keshavarzi, Mustapha Ahmed Yusuf, Amir Hassanzadeh, Narges Marvi, Marzia Shahriari-Namadi, Zahra Nassiri
    Oriental Insects.2017; 51(4): 345.     CrossRef
  • Contamination of cockroaches (Insecta: Blattaria) to medically fungi: A systematic review and meta-analysis
    H. Nasirian
    Journal de Mycologie Médicale.2017; 27(4): 427.     CrossRef
  • The cockroach Periplaneta americana as a potential paratenic host of the lungworm Aelurostrongylus abstrusus
    Luigi Falsone, Vito Colella, Ettore Napoli, Emanuele Brianti, Domenico Otranto
    Experimental Parasitology.2017; 182: 54.     CrossRef
  • The prevalence of protozoa in the gut of German cockroaches (Blattella germanica) with special reference to Lophomonas blattarum
    Rafael Martínez-Girón, Cristina Martínez-Torre, Hugo Cornelis van Woerden
    Parasitology Research.2017; 116(11): 3205.     CrossRef
  • Cockroaches as a Source of High Bacterial Pathogens with Multidrug Resistant Strains in Gondar Town, Ethiopia
    Feleke Moges, Setegn Eshetie, Mengistu Endris, Kahsay Huruy, Dagnachew Muluye, Tigist Feleke, Fisha G/Silassie, Getenet Ayalew, Raja Nagappan
    BioMed Research International.2016; 2016: 1.     CrossRef
  • Cockroaches (Blattodea: Blattidae): A Reservoir of Pathogenic Microbes in Human-Dwelling Localities in Lahore
    H. Memona, F. Manzoor, A. A. Anjum
    Journal of Medical Entomology.2016; : tjw168.     CrossRef
  • 9,462 View
  • 125 Download
  • 19 Web of Science
  • Crossref

Brief Communications

Detection of Gnathostoma spinigerum Antibodies in Sera of Non-Traumatic Subarachnoid Hemorrhage Patients in Thailand
Amnat Kitkhuandee, Waranon Munkong, Kittisak Sawanyawisuth, Penchom Janwan, Wanchai Maleewong, Pewpan M. Intapan
Korean J Parasitol 2013;51(6):755-757.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.755

Gnathostoma spinigerum can cause subarachnoid hemorrhage (SAH). The detection of specific antibodies in serum against G. spinigerum antigen is helpful for diagnosis of neurognathostomiasis. There is limited data on the frequency of G. spinigerum infection in non-traumatic SAH. A series of patients diagnosed as non-traumatic SAH at the Srinagarind Hospital, Khon Kaen University, Thailand between January 2011 and January 2013 were studied. CT or MR imaging of the brain was used for diagnosis of SAH. Patients were categorized as aneurysmal subarachnoid hemorrhage (A-SAH) or non-aneurysmal subarachnoid hemorrhage (NA-SAH) according to the results of cerebral angiograms. The presence of specific antibodies in serum against 21- or 24-kDa G. spinigerum antigen was determined using the immunoblot technique. The detection rate of antibodies was compared between the 2 groups. Of the 118 non-traumatic SAH patients for whom cerebral angiogram and immunoblot data were available, 80 (67.8%) patients had A-SAH, whereas 38 (32.2%) had NA-SAH. Overall, 23.7% were positive for specific antibodies against 21- and/or 24-kDa G. spinigerum antigen. No significant differences were found in the positive rate of specific antibodies against G. spinigerum in both groups (P-value=0.350).

  • 9,566 View
  • 76 Download
A Recombinant Matrix Metalloproteinase Protein from Gnathostoma spinigerum for Serodiagnosis of Neurognathostomiasis
Penchom Janwan, Pewpan M. Intapan, Hiroshi Yamasaki, Porntip Laummaunwai, Kittisak Sawanyawisuth, Chaisiri Wongkham, Chatchai Tayapiwatana, Amnat Kitkhuandee, Viraphong Lulitanond, Yukifumi Nawa, Wanchai Maleewong
Korean J Parasitol 2013;51(6):751-754.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.751

Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components of molecular mass 21 kDa and 24 kDa in larval extracts of Gnathostoma spinigerum (Gs 21/24), has high sensitivity and specificity for diagnosis of neurognathostomiasis. However, only very small amounts of the Gs 21/24 antigens can be prepared from parasites harvested from natural or experimental animals. To overcome this problem, we recently produced a recombinant matrix metalloproteinase (rMMP) protein from G. spinigerum. In this study, we evaluated this rMMP alongside the Gs 21/24 antigens for serodiagnosis of human neurognathostomiasis. We studied sera from 40 patients from Srinagarind Hospital, Khon Kaen University, Thailand, with clinical criteria consistent with those of neurognathostomiasis, and sera from 30 healthy control adults from Thailand. All sera were tested for specific IgG antibodies against both G. spinigerum crude larval extract and rMMP protein using immunoblot analysis. The sensitivity and specificity for both antigenic preparations were all 100%. These results show that G. spinigerum rMMP protein can be used as an alternative diagnostic antigen, in place of larval extract, for serodiagnosis of neurognathostomiasis.

Citations

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  • Protein and antigen profiles of third-stage larvae of Gnathostoma spinigerum assessed with next-generation sequencing transcriptomic information
    Kathyleen Nogrado, Tipparat Thiangtrongjit, Poom Adisakwattana, Paron Dekumyoy, Sant Muangnoicharoen, Charin Thawornkuno, Onrapak Reamtong
    Scientific Reports.2022;[Epub]     CrossRef
  • Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis
    Penchom Janwan, Pewpan M. Intapan, Lakkhana Sadaow, Rutchanee Rodpai, Hiroshi Yamasaki, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Phuangphaka Sadee, Wanchai Maleewong
    Diagnostics.2021; 11(5): 862.     CrossRef
  • Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development
    Tipparat Thiangtrongjit, Kathyleen Nogrado, Thawatchai Ketboonlue, Preeyarat Malaitong, Poom Adisakwattana, Onrapak Reamtong
    Pathogens.2021; 10(9): 1080.     CrossRef
  • Combining lexical and context features for automatic ontology extension
    Sara Althubaiti, Şenay Kafkas, Marwa Abdelhakim, Robert Hoehndorf
    Journal of Biomedical Semantics.2020;[Epub]     CrossRef
  • Human gnathostomiasis: a neglected food-borne zoonosis
    Guo-Hua Liu, Miao-Miao Sun, Hany M. Elsheikha, Yi-Tian Fu, Hiromu Sugiyama, Katsuhiko Ando, Woon-Mok Sohn, Xing-Quan Zhu, Chaoqun Yao
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Surveillance and diagnosis of zoonotic foodborne parasites
    Reza Zolfaghari Emameh, Sami Purmonen, Antti Sukura, Seppo Parkkila
    Food Science & Nutrition.2018; 6(1): 3.     CrossRef
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Original Article

Modulation of Antibody Responses against Gnathostoma spinigerum in Mice Immunized with Crude Antigen Formulated in CpG Oligonucleotide and Montanide ISA720
Pewpan M. Intapan, Chakrit Hirunpetcharat, Churairat Kularbkaew, Wiboonchai Yutanawiboonchai, Penchom Janwan, Wanchai Maleewong
Korean J Parasitol 2013;51(6):637-644.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.637

This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.

Citations

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  • Evaluation of immunodiagnostic tests for human gnathostomiasis using different antigen preparations of Gnathostoma spinigerum larvae against IgE, IgM, IgG, IgG1‐4 and IgG1 patterns of post‐treated patients
    Issariya Ieamsuwan, Dorn Watthanakulpanich, Urai Chaisri, Poom Adisakwattana, Paron Dekumyoy
    Tropical Medicine & International Health.2021; 26(12): 1634.     CrossRef
  • 8,761 View
  • 86 Download
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Case Reports

Cutaneous Gnathostomiasis with Recurrent Migratory Nodule and Persistent Eosinophilia: a Case Report from China
Jing Cui, Ye Wang, Zhong Quan Wang
Korean J Parasitol 2013;51(4):467-470.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.467

The present study reports a human case of cutaneous gnathostomiasis with recurrent migratory nodule and persistent eosinophilia in China. A 52-year-old woman from Henan Province, central China, presented with recurrent migratory reddish swelling and subcutaneous nodule in the left upper arm and on the back for 3 months. Blood examination showed eosinophila (21.2%), and anti-sparganum antibodies were positive. Skin biopsy of the lesion and histopathological examinations revealed dermal infiltrates of eosinophils but did not show any parasites. Thus, the patient was first diagnosed as sparganosis; however, new migratory swellings occurred after treatment with praziquantel for 3 days. On further inquiring, she recalled having eaten undercooked eels and specific antibodies to the larvae of Gnathostoma spinigerum were detected. The patient was definitely diagnosed as cutaneous gnathostomiasis caused by Gnathostoma sp. and treated with albendazole (1,000 mg/day) for 15 days, and the subsequent papule and blister developed after the treatment. After 1 month, laboratory findings indicated a reduced eosinophil count (3.3%). At her final follow-up 18 months later, the patient had no further symptoms and anti-Gnathostoma antibodies became negative. Conclusively, the present study is the first report on a human case of cutaneous gnathostomiasis in Henan Province, China, based on the past history (eating undercooked eels), clinical manifestations (migratory subcutaneous nodule and persistent eosinophilia), and a serological finding (positive for specific anti-Gnathostoma antibodies).

Citations

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  • First reported case of intraocular gnathostomiasis in China
    Shuang Wang, Ling Zhang, Feng Miao, Jun Huang, Juan Guo
    Journal of Travel Medicine.2022;[Epub]     CrossRef
  • Current and future strategies against cutaneous parasites
    Ernest Man, Helen P. Price, Clare Hoskins
    Pharmaceutical Research.2022; 39(4): 631.     CrossRef
  • Eosinophilic Meningitis (Angiostrongylus spp. and Gnathostoma spp.)
    Nelson Iván Agudelo Higuita, Jackrapong Bruminhent
    Current Tropical Medicine Reports.2022; 9(1): 40.     CrossRef
  • Morphological and Phylogenetic Analysis of Eustrongylides sp. and Gnathostoma spinigerum Parasitizing the Asian Swamp Eel Monopterusalbus in China
    Sixin Zhang, Guangping Huang, Liang Li, Xianyong Liu, Xiaoli Tang, Xun Suo
    Pathogens.2021; 10(6): 711.     CrossRef
  • Human gnathostomiasis: a neglected food-borne zoonosis
    Guo-Hua Liu, Miao-Miao Sun, Hany M. Elsheikha, Yi-Tian Fu, Hiromu Sugiyama, Katsuhiko Ando, Woon-Mok Sohn, Xing-Quan Zhu, Chaoqun Yao
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Imported gnathostomiasis manifesting as cutaneous larva migrans and Löffler’s syndrome
    William L. Hamilton, Daniel Agranoff
    BMJ Case Reports.2018; 2018: bcr-2017-223132.     CrossRef
  • 12,918 View
  • 175 Download
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Gnathostoma spinigerum Infection in the Upper Lip of a Korean Woman: An Autochthonous Case in Korea
Jae Hee Kim, Hyemi Lim, Young-Sang Hwang, Tae Yeon Kim, Eun Mee Han, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2013;51(3):343-347.
Published online June 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.3.343

Autochthonous human gnathostomiasis had never been reported in the Republic of Korea. We report here a case of Gnathostoma spinigerum infection in a 32-year-old Korean woman, presumed to have been infected via an indigenous route. The patient had experienced a painful migratory swelling near the left nasolabial fold area of the face for a year, with movement of the swelling to the mucosal area of the upper lip 2 weeks before surgical removal of the lesion. Histopathological examinations of the extracted tissue revealed inflammation with heavy eosinophilic infiltrations and sections of a nematode suggestive of a Gnathostoma sp. larva. The larva characteristically revealed about 25 intestinal cells with multiple (3-6) nuclei in each intestinal cell consistent with the 3rd-stage larva of G. spinigerum. The patient did not have any special history of travel abroad except a recent trip, 4 months before surgery, to China where she ate only cooked food. The patient is the first recorded autochthonous case of G. spinigerum infection in Korea.

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  • Imported parasitic diseases in the Republic of Korea: status and issues
    Jong-Yil Chai
    Journal of the Korean Medical Association.2025; 68(1): 52.     CrossRef
  • Parasitic Diseases of Upper Gastrointestinal Tract
    Heung Up Kim
    The Korean Journal of Helicobacter and Upper Gastrointestinal Research.2024; 24(2): 127.     CrossRef
  • A case of vocal cord gnathostomiasis diagnosed with sectional morphologies in a histopathological specimen from a Chinese woman living in Korea
    Doo Sik Park, Eun Hyun Cho, Kyung Hoon Park, Soo Min Jo, Bumjung Park, Sun Huh
    Parasites, Hosts and Diseases.2023; 61(3): 298.     CrossRef
  • Morphological and Phylogenetic Analysis of Eustrongylides sp. and Gnathostoma spinigerum Parasitizing the Asian Swamp Eel Monopterusalbus in China
    Sixin Zhang, Guangping Huang, Liang Li, Xianyong Liu, Xiaoli Tang, Xun Suo
    Pathogens.2021; 10(6): 711.     CrossRef
  • Human gnathostomiasis: a neglected food-borne zoonosis
    Guo-Hua Liu, Miao-Miao Sun, Hany M. Elsheikha, Yi-Tian Fu, Hiromu Sugiyama, Katsuhiko Ando, Woon-Mok Sohn, Xing-Quan Zhu, Chaoqun Yao
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Gnathostomiasis: an emerging infectious disease relevant to all dermatologists
    Francisco Bravo, Bernardo Gontijo
    Anais Brasileiros de Dermatologia.2018; 93(2): 172.     CrossRef
  • Imported gnathostomiasis manifesting as cutaneous larva migrans and Löffler’s syndrome
    William L. Hamilton, Daniel Agranoff
    BMJ Case Reports.2018; 2018: bcr-2017-223132.     CrossRef
  • Gnathostomiasis: an Emerging Infection of Raw Fish Consumers in Gnathostoma Nematode-Endemic and Nonendemic Countries
    James H. Diaz
    Journal of Travel Medicine.2015; 22(5): 318.     CrossRef
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  • 110 Download
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Original Articles

Positivity and Intensity of Gnathostoma spinigerum Infective Larvae in Farmed and Wild-Caught Swamp Eels in Thailand
Wilai Saksirisampant, Benjamas Wongsatayanon Thanomsub
Korean J Parasitol 2012;50(2):113-118.
Published online May 24, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.2.113

From July 2008 to June 2009, livers of the swamp eels (Monopterus alba) were investigated for advanced third-stage larvae (AL3) of Gnathostoma spinigerum. Results revealed that 10.2% (106/1,037) and 20.4% (78/383) of farmed eels from Aranyaprathet District, Sa Kaeo Province and those of wild-caught eels obtained from a market in Min Buri District of Bangkok, Thailand were infected, respectively. The prevalence was high during the rainy and winter seasons. The infection rate abruptly decreased in the beginning of summer. The highest infection rate (13.7%) was observed in September and absence of infection (0%) in March-April in the farmed eels. Whereas, in the wild-caught eels, the highest rate (30.7%) was observed in November, and the rate decreased to the lowest at 6.3% in March. The average no. (mean±SE) of AL3 per investigated liver in farmed eels (1.1±0.2) was significantly lower (P=0.040) than those in the caught eels (0.2±0.03). In addition, the intensity of AL3 recovered from each infected liver varied from 1 to 18 (2.3±0.3) in the farmed eels and from 1 to 47 (6.3±1.2) in the caught eels, respectively. The AL3 intensity showed significant difference (P=0.011) between these 2 different sources of eels. This is the first observation that farmed eels showed positive findings of G. spinigerum infective larvae. This may affect the standard farming of the culture farm and also present a risk of consuming undercooked eels from the wild-caught and farmed eels.

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  • Human gnathostomiasis: A review on the biology of the parasite with special reference on the current therapeutic management
    Kathyleen Nogrado, Poom Adisakwattana, Onrapak Reamtong
    Food and Waterborne Parasitology.2023; 33: e00207.     CrossRef
  • Morphological and Phylogenetic Analysis of Eustrongylides sp. and Gnathostoma spinigerum Parasitizing the Asian Swamp Eel Monopterusalbus in China
    Sixin Zhang, Guangping Huang, Liang Li, Xianyong Liu, Xiaoli Tang, Xun Suo
    Pathogens.2021; 10(6): 711.     CrossRef
  • Molecular cloning and characterization of serine protease inhibitor from food-borne nematode, Gnathostoma spinigerum
    Anusorn Tinyou, Salisa Chaimon, Orawan Phuphisut, Porntida Kobpornchai, Preeyarat Malaithong, Akkarin Poodeepiyasawat, Issariya Ieamsuwan, Jiraporn Ruangsittichai, Pornpan Pumirat, Paron Dekumyoy, Onrapak Reamtong, Poom Adisakwattana
    Acta Tropica.2020; 204: 105288.     CrossRef
  • Infection Status of Gnathostoma spinigerum Larvae in Asian Swamp Eels, Monopterus albus, Purchased from Local Markets in Cambodia
    Jong-Yil Chai, Bong-Kwang Jung, Keon Hoon Lee, Sung-Jong Hong, Virak Khieu, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2020; 58(6): 695.     CrossRef
  • Genetic diversity of infective larvae of Gnathostoma spinigerum (Nematoda: Gnathostomatidae) in freshwater swamp eels from Thailand
    P. Eamsobhana, D. Wanachiwanawin, K. Roongruangchai, S.L. Song, H.S. Yong
    Journal of Helminthology.2017; 91(6): 767.     CrossRef
  • Cloning and expression analysis of two pro-inflammatory cytokines, IL-1β and its receptor, IL-1R2, in the Asian swamp eel Monopterus albus
    Q. Q. Xu, P. Xu, J. W. Zhou, T. S. Pan, R. Tuo, K. Ai, D. Q. Yang
    Molecular Biology.2016; 50(5): 671.     CrossRef
  • Antiviral genes in the Asian swamp eel Monopterus albus: comparison of tissue expression and inducible expression of six interferon regulatory factors
    Qiaoqing Xu, Jin Wan, Tingshuang Pan, Jingwen Zhou, Kete Ai, Daiqin Yang
    Genes & Genomics.2015; 37(5): 429.     CrossRef
  • Larval Gnathostoma spinigerum Detected in Asian Swamp Eels, Monopterus albus, Purchased from a Local Market in Yangon, Myanmar
    Jong-Yil Chai, Woon-Mok Sohn, Byoung-Kuk Na, Jong-Bok Park, Hoo-Gn Jeoung, Eui-Hyug Hoang, Thi Thi Htoon, Htay Htay Tin
    The Korean Journal of Parasitology.2015; 53(5): 619.     CrossRef
  • Cutaneous Gnathostomiasis with Recurrent Migratory Nodule and Persistent Eosinophilia: a Case Report from China
    Jing Cui, Ye Wang, Zhong Quan Wang
    The Korean Journal of Parasitology.2013; 51(4): 467.     CrossRef
  • 10,812 View
  • 95 Download
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Assessment of Loiasis and Outcomes of Ivermectin Masstreatment in Ijebu-North, Nigeria
A. A. Hassan, B. Akinsanya, N. Iyase, F. O. Owagboriaye
Korean J Parasitol 2011;49(2):153-159.
Published online June 14, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.2.153

A total of 286 individuals from 3 selected communities (Areedi-Aje, Ipakodo/Ojokodo, and Ijebu-Igbo) of Ijebu-North, southwestern Nigeria were examined for Loa loa microfilaremia using finger prick blood smear, between December 2008 and March 2009. Rapid assessment procedure for loiasis (RAPLOA) was used to obtain information, from 187 Ijebu-Igbo residents, on adverse reactions experienced from retrospective treatments with ivermectin and history of eye worm. Only 33.9% of the respondents reported having had a history of eye worm while 33.2% had microfilaremia. The demographic factor of gender was not significant determinants of the prevalence (P>0.05) while age was significant (P<0.05). The highest prevalence of eye worm history and microfilaremia were recorded in 61-70 and 15-20 years of age categories, respectively. Ijebu-Igbo had 27.3% eye worm history, 32.1% microfilaremia, and the highest intensity of 140 microfilariae (mf)/ml. Ipakodo area had the highest eye worm history of 54.4% and the highest intensity of 420 mf/ml. Areedi-Aje had the highest occurrence of 45.2% microfilaremia and the highest intensity of 460 mf/ml. Predictably, Areedi-Aje and Ipakodo areas were high risk communities. The low intensity of L. loa infection with an insignificant (2.1%; P>0.05) adverse reactions from 187 subjects involved in the retrospective ivermectin administration confirmed that ivermectin delivery may be considered safe. The community-directed treatment with ivermectin (CDTI) programme was most probably responsible for the low prevalence and intensity.

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  • In Southern Nigeria Loa loa Blood Microfilaria Density is Very Low Even in Areas with High Prevalence of Loiasis: Results of a Survey Using the New LoaScope Technology
    Emmanuel Emukah, Lindsay J. Rakers, Barminas Kahansim, Emmanuel S. Miri, Bertram E. B. Nwoke, Emily Griswold, Yisa Saka, Ifeoma Anagbogu, Emmanuel Davies, Cephas Ityonzughul, Michael D’Ambrosio, Matthew Bakalar, Daniel A. Fletcher, Thomas Nutman, Joseph K
    The American Journal of Tropical Medicine and Hygiene.2018; 99(1): 116.     CrossRef
  • A Case of Loiasis in a Patient Returning to China Diagnosed by Nested PCR Using DNA Extracted From Tissue
    Xiaohui Wang, Xiaoke Zhang, Zhiyong Zong
    Journal of Travel Medicine.2012; 19(5): 314.     CrossRef
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  • 74 Download
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Brief Communications

Prevalence of Malaria in Pregnant Women in Lagos, South-West Nigeria
Chimere O. Agomo, Wellington A. Oyibo, Rose I. Anorlu, Philip U. Agomo
Korean J Parasitol 2009;47(2):179-183.
Published online May 27, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.2.179

Prevalence rates reported for malaria in pregnancy in Nigeria vary considerably. The accuracy of results of malaria diagnosis is dependent on training, experience, and motivation of the microscopist as well as the laboratory facility available. Results of training programmes on malaria microscopy have shown low levels of sensitivity and specificity of those involved in malaria diagnosis routinely and for research. This study was done to ascertain the true prevalence of malaria in pregnancy in Lagos, South-West Nigeria. A total of 1,084 pregnant women were recruited into this study. Blood smears stained with Giemsa were used for malaria diagnosis by light microscopy. Malaria infection during pregnancy presents mostly as asymptomatic infection. The prevalence of malaria in this population was 7.7% (95% confidence interval; 6.2-9.4%). Factors identified to increase the risk of malaria infection include young maternal age (< 20 years), and gravidity (primigravida). In conclusion, this study exposes the over-diagnosis of malaria in pregnancy and the need for training and retraining of laboratory staffs as well as establishing the malaria diagnosis quality assurance programme to ensure the accuracy of malaria microscopy results at all levels.

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Detection of Gnathostoma spinigerum Third-Stage Larvae in Snakeheads Purchased from a Central Part of Myanmar
Bong-Kwang Jung, Jin-Ju Lee, Kyoung-Ho Pyo, Hyeong-Jin Kim, Hoo-Gn Jeong, Cheong-Ha Yoon, Soon-Hyung Lee, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2008;46(4):285-288.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.285

To examine the infection status of freshwater fish with Gnathostoma spp. larvae in Myanmar, we purchased 15 snakeheads, Channa striatus, from a local market in a suburban area of Naypyidaw, the new capital city. Two larval gnathostomes were collected using an artificial digestion technique, and observed by a light microscope and a scanning electron microscope. The size of an intact larva was 2.65 mm long and 0.32 mm wide. The characteristic morphology of the larvae included the presence of a long esophagus (0.80 mm long), 2 pairs of cervical sacs (0.43 mm long), and a characteristic head bulb with 4 rows of hooklets. The number of hooklets in the 1st, 2nd, 3rd, and 4th row was 45, 48, 50, and 52, respectively. Based on these morphological characters, the larvae were identified as the advanced 3rd-stage larvae of Gnathostoma spinigerum. This is the first report of detection of G. spinigerum 3rd-stage larvae in the central part of Myanmar. Our study suggests that intake of raw meat of snakehead fish in Myanmar may result in human gnathostomiasis.

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Intestinal helminthiases and schistosomiasis among school children in an urban center and some rural communities in southwest Nigeria
Olufemi Moses Agbolade, Ndubuisi Chinweike Agu, Oluseyi Olusegun Adesanya, Adedayo Olugbenga Odejayi, Aliu Adekunle Adigun, Emmanuel Babatunde Adesanlu, Flourish George Ogunleye, Adetoun Omolayo Sodimu, Stella Ajoke Adeshina, Ganiyat Olusola Bisiriyu, Oluwatosin Ibiyemi Omotoso, Karen Mfon Udia
Korean J Parasitol 2007;45(3):233-238.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.233

Intestinal helminths and schistosomiasis among school children were investigated in an urban and some rural communities of Ogun State, southwest Nigeria. Fecal samples of 1,059 subjects (524 males, 535 females) aged 3-18 years were examined using direct smear and brine concentration methods between June 2005 and November 2006. The pooled prevalence of infection was 66.2%. Ascaris lumbricoides showed the highest prevalence (53.4%) (P < 0.001) followed by hookworms (17.8%), Trichuris trichiura (10.4%), Taenia sp. (9.6%), Schistosoma mansoni (2.3%), Strongyloides stercoralis (0.7%), Schistosoma haematobium (0.6%), and Enterobius vermicularis (0.3%). The prevalences of A. lumbricoides, hookworms, Taenia sp., S. mansoni, and S. stercoralis in the urban centre were similar (P > 0.05) to those in the rural communities. The fertile and infertile egg ratios of A. lumbricoides in the urban centre and the rural communities were 13: 1 and 3.7: 1, respectively. Each helminth had similar prevalences among both genders (P > 0.05). The prevalence of A. lumbricoides increased significantly with age (P < 0.001). The commonest double infections were Ascaris and hookworms, while the commonest triple infections were Ascaris, hookworms, and Trichuris. The study demonstrates the need for urgent intervention programmes against intestinal helminthiases and schistosomiasis in the study area.

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Original Article
Biting of anthropophilic Culicoides fulvithorax (Diptera: Ceratopogonidae), a vector of Mansonella perstans in Nigeria
Olufemi-Moses Agbolade, Dora Olufunmilola Akinboye, Taiwo Monroof Olateju, Oluwatoyin Adepeju Ayanbiyi, Olukayode Olakunle Kuloyo, Oluwalolami Oluwaseun Fenuga
Korean J Parasitol 2006;44(1):67-72.
Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.67

Anthropophilic Culicoides were investigated in a rural community endemic for Mansonella perstans in Ijebu North area of western Nigeria between December 2003 and October 2004. Three hundred and fifty-nine adults of Culicoides fulvithorax collected by human bait in the morning were dissected for Mansonella perstans larvae, and 1.95% of infection rate was found. Seasonal abundance of C. fulvithorax was investigated by monthly biting rates, and showed that higher prevalence was observed in rainy season, with peak in September. Culicoides prevalence was positively correlated with rainfall and relative humidity, but not temperature. Human perceptions on the behavior of these biting midges were determined by interviewing 854 self-selected villagers, of which 86.5% of the interviewees confessed having experienced Culicoides bites. Between 76.5 and 99.1% of the various age groups complained body reactions to Culicoides bites. Itching was the most frequent body reaction. No interviewees associated Culicoides with transmission of any parasitic infections. The results showed need to adequately control Culicoides in the community.

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