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"Seung-Yull Cho"

Review

What was the main factor in successful control of ascariasis in Korea?
Seung-Yull Cho, Sung-Tae Hong
Parasites Hosts Dis 2023;61(2):103-126.
Published online May 23, 2023
DOI: https://doi.org/10.3347/PHD.22152
In the l950s, under the legacy of traditional agriculture, Ascaris lumbricoides, spread epidemically in the war-bitten society of Korea. Consensus on the parasite control was drafted in the Parasite Disease Prevention Act, which passed a parliamentary agreement in 1966, and established safe disposal of feces and mass chemotherapy as control strategies. Biannual stool examinations and treating infected schoolchildren were basic scheme of the control activity through which revenue could be secured for organized business. In the 27 years following 1969, a maximum of 16 million stool examinations had been done every year. Cellophane thick smear enabled the task. The infection declined remarkably in the 1970s when industrialization and green revolution proceeded. A population study of A. lumbricoides in the late 1970s helped us better understand its epidemiology. The data also settled down the understandable protest of teachers against the repeated stool examinations. In the 9 years following 1987, the target population was gradually reduced when the egg positive rate was below 0.1%. An article in the Korean Law, stipulating obligatory stool examinations, was made optional. Although the long-term Korean effort of Ascaris control was a success, the effect of mass chemotherapy was not as succinct in terms of lowering reinfection. In the period of control, Korean agricultural technology changed, and the economy grew and supplied sanitary facilities by which the vicious cycle was disconnected. Reduction of morbidity was a benefit of mass chemotherapy, which is the only control method feasible in economically difficult countries. The most important hurdle of parasite control in the 1960s was poverty of general population and limited financial resources in Korea but the society formed a consensus on the priority of intestinal helminthiasis control during the ordeal period. The national consensus in the 1960s was the critical milestone for Ascaris control in Korea. Under the social agreement, application of timely technical and research advancements in parasitology achieved the success of ascariasis elimination. The successful experience of ascariasis elimination in Korea can be a benchmark for countries where neglected tropical diseases are endemically recycled.

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  • Clonorchis sinensis and Cholangiocarcinoma
    Eun-Min Kim, Sung-Tae Hong
    Journal of Korean Medical Science.2025;[Epub]     CrossRef
  • 7,247 View
  • 204 Download
  • 1 Web of Science
  • Crossref

Mini Review

Fifty Years of the Korean Society for Parasitology
Seung-Yull Cho
Korean J Parasitol 2009;47(Suppl):S7.
Published online October 26, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.S.S7

In 1959, the Korean Society for Parasitology was founded by clinical scientists, specialists of public health, and 5 core parasitologists with experience in American science and medicine. The Society this year celebrates its 50th anniversary. Due to public health importance at the time of foundation, medical parasitology was the main stream for next 3 decades. Domestic problems of niche parasitic diseases, unlisted in 6 tropical diseases of major importance, had been studied by own efforts. To cope with the demand of parasite control, evaluation system for control activity was built up. Control activity against soil-transmitted nematodes, conducted for almost 3 decades, was evaluated as a success. Evaluation of praziquantel efficacy for clonorchiasis, paragonimiasis, and neurocysticercosis, population dynamics of Ascaris lumbricoides infection in a situation of continuous reinfections, diagnostic modalities of antibody tests combined with brain imaging developed for helminthiasis of the central nervous system and researches on intestinal trematodes were achievements in the first 30 years. During the recent 2 decades, science researches, such as cell and molecular biology of parasites and immunology of parasitic infections have been studied especially on parasitic allergens and proteolytic and anti-oxidant enzymes. Experiences of international cooperation for world health have been accumulated and would be expanded in the future.

Citations

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  • Functional characterization of glucose transporter 4 involved in glucose uptake in Clonorchis sinensis
    Hojong Jun, Ernest Mazigo, Wang-Jong Lee, Yun-Kyu Park, Jin-Hee Han, Seok Ho Cha
    Parasites, Hosts and Diseases.2024; 62(4): 450.     CrossRef
  • Laparoscopic excision for ectopic peritoneal paragonimiasis mimicking a gastric duplication cyst: A case report
    Chul Kyu Roh, Min Jung Jung
    Annals of Medicine and Surgery.2021; 69: 102754.     CrossRef
  • 9,632 View
  • 108 Download
  • Crossref

Original Articles

A seroepidemiological survey of Taenia solium cysticercosis in Nabo, Guangxi Zhuang Autonomous Region, China
Joon-Yong Chung, Keeseon S. Eom, Yichao Yang, Xenming Li, Zheng Feng, Han-Jong Rim, Seung-Yull Cho, Yoon Kong
Korean J Parasitol 2005;43(4):135-139.
Published online December 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.4.135

We have observed the seropositive rate of Taenia solium cysticercosis in residents at Nabo Village, Tiandong County, Guangxi Zhuang Autonomous Region, China by enzyme linked immunosorbent assay. The village had been found to be a relatively high endemic area of porcine cysticercosis among roaming pigs. Of 202 persons examined four males aged 15, 25, 35 and 41 year-old exhibited absorbance (abs) at 0.18, 0.20, 0.35 and 0.55, respectively. In addition, two females whose ages were 35 and 39 years revealed specific antibody levels of abs 0.26 and 0.41 in their sera. Overall positive rate among the people was 2.97%. All of these persons agreed that they had ingested the pork infected with T. solium metacestode (TsM), while history of proglottid discharge was not noticed from all of them. Three males and one female complained of intermittent headache. Our findings reinforced not only that the prevalence of cysticercosis might be related with roaming pigs infected with TsM but also that behavioral and environmental practices in local community constituted risk factors for transmission of the infection.

Citations

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  • A Marked Decline in Taenia solium Taeniasis and Cysticercosis Infections in China: Possible Reasons from the Ecological Determinants Perspective
    Junqiang Li, Feifei Xu, Yuancai Chen, Md Robiul Karim, Yilin Wang, Kaihui Zhang, Xiaoying Li, Pitambar Dhakal, Rongjun Wang, Longxian Zhang
    Zoonoses.2023;[Epub]     CrossRef
  • The burden of T. solium cysticercosis and selected neuropsychiatric disorders in Mocuba district, Zambézia province, Mozambique
    Irene Langa, Fernando Padama, Noémia Nhancupe, Alberto Pondja, Delfina Hlashwayo, Lidia Gouveia, Dominik Stelzle, Clarissa Prazeres da Costa, Veronika Schmidt, Andrea S. Winkler, Emília Virgínia Noormahomed, Eduardo Torres
    PLOS Neglected Tropical Diseases.2022; 16(7): e0010606.     CrossRef
  • Taeniasis and cysticercosis in Asia: A review with emphasis on molecular approaches and local lifestyles
    Akira Ito, Tiaoying Li, Toni Wandra, Paron Dekumyoy, Tetsuya Yanagida, Munehiro Okamoto, Christine M Budke
    Acta Tropica.2019; 198: 105075.     CrossRef
  • Infections of Soil-Transmitted Helminth in Refugees from North Korea
    Young-Il Lee, Min Seo, Suk-Bae Kim
    The Korean Journal of Parasitology.2018; 56(3): 291.     CrossRef
  • Zoonoses in South-East Asia: a regional burden, a global threat
    Marion Bordier, François Roger
    Animal Health Research Reviews.2013; 14(1): 40.     CrossRef
  • Prevalence and Risk Factors Associated with Human Taenia Solium Infections in Mbozi District, Mbeya Region, Tanzania
    Gloria Mwanjali, Charles Kihamia, Deodatus Vitalis Conatus Kakoko, Faustin Lekule, Helena Ngowi, Maria Vang Johansen, Stig Milan Thamsborg, Arve Lee Willingham, Malcolm K. Jones
    PLoS Neglected Tropical Diseases.2013; 7(3): e2102.     CrossRef
  • Serodiagnostic reliability of single-step enriched low-molecular weight proteins of Taenia solium metacestode of American and Asian isolates
    E.-G. Lee, Y.-A. Bae, S.-H. Kim, S.P. Díaz-Camacho, Y. Nawa, Y. Kong
    Transactions of the Royal Society of Tropical Medicine and Hygiene.2010; 104(10): 676.     CrossRef
  • Seroprevalence of Taenia solium antibodies in Vellore district, south India
    V. Prabhakaran, M. Venkata Raghava, V. Rajshekhar, J. Muliyil, A. Oommen
    Transactions of the Royal Society of Tropical Medicine and Hygiene.2008; 102(3): 246.     CrossRef
  • What triggers seizures in neurocysticercosis? A MRI-based study in pig farming community from a district of North India
    Amit Prasad, Rakesh K. Gupta, Sunil Pradhan, Mukesh Tripathi, Chandra M. Pandey, Kashi N. Prasad
    Parasitology International.2008; 57(2): 166.     CrossRef
  • Tissue parasitic helminthiases are prevalent at Cheongjin, North Korea
    Chenghua Shen, Shunyu Li, Shanzi Zheng, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
    The Korean Journal of Parasitology.2007; 45(2): 139.     CrossRef
  • 8,619 View
  • 71 Download
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Excretory bladder: the source of cysteine proteases in Paragonimus westermani metacercariae
Hyun-Jong Yang, Young-Bae Chung, Shin-Yong Kang, Yoon Kong, Seung-Yull Cho
Korean J Parasitol 2002;40(2):89-92.
Published online June 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.2.89

The cysteine proteases of Paragonimus westermani metacercariae are involved in metacercarial excystment, host immune modulation, and possibly in tissue penetration. In order to clarify the origin of the enzymes, 28 and 27 kDa cysteine proteases in metacercarial excretory-secretory products were purified through the FPLC system using Mono Q column chromatography. The polyclonal antibodies to the enzymes were produced in BALB/c mice. Immunolocalization studies revealed that both cysteine proteases were distributed at the linings of excretory bladder and excretory concretions of the metacercariae. It was suggested that the excretory epithelium of P. westermani undertake the secretory function of metacercarial cysteine proteases, in addition to its role as a route for eliminating waste products.

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  • Early Cysteine Protease Activity in Excretory Bladder Triggers Metacercaria Excystment of Paragonimus westermani
    Y. B. Chung, T. S. Kim, H. J. Yang
    Journal of Parasitology.2005; 91(4): 953.     CrossRef
  • 7,669 View
  • 69 Download
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Incidence patterns of vivax malaria in civilians residing in a high-risk county of Kyonggi-do (Province), Republic of Korea
Jung Ju Moon, Seung-Yull Cho
Korean J Parasitol 2001;39(4):293-299.
Published online December 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.4.293

The characteristics of vivax malaria epidemics along the demilitarized zone (DMZ) in the Republic of Korea has been established by the early surveillance data. To further characterize the epidemic, data of civilian patients microscopically diagnosed with malaria from 1995 through 2000 were analyzed in Yonchon-gun (county). Malaria incidence was greater in male civilians > 30 years of age (p < 0.05). The annual parasite index was significantly higher in those living in the administrative areas (Myeon) traversed by DMZ than those living in Myons not traversed by DMZ (p < 0.05). Analysis according to the distance (4 to 14 km) from DMZ showed that people living in villages close to DMZ had higher annual parasite indices than those living in villages remote from DMZ (p for trend < 0.05). Civilians living in Myeons with plains and located in northwestern part of the county had higher annual parasite indices than those living in hilly Myeons located in southeastern part of the county (p for trend < 0.05). These findings suggest that the contraction of vivax malaria is related with night-time outdoor activities, and that the distance from DMZ is a risk factor. In this area, the flying distance of infected vector mosquitos can explain the annually repeating occurrence of civilian cases.

Citations

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  • Sex-based differences in clearance of chronic Plasmodium falciparum infection
    Jessica Briggs, Noam Teyssier, Joaniter I Nankabirwa, John Rek, Prasanna Jagannathan, Emmanuel Arinaitwe, Teun Bousema, Chris Drakeley, Margaret Murray, Emily Crawford, Nicholas Hathaway, Sarah G Staedke, David Smith, Phillip J Rosenthal, Moses Kamya, Gra
    eLife.2020;[Epub]     CrossRef
  • Application of Epidemiological Geographic Information System: An Open-Source Spatial Analysis Tool Based on the OMOP Common Data Model
    Jaehyeong Cho, Seng Chan You, Seongwon Lee, DongSu Park, Bumhee Park, George Hripcsak, Rae Woong Park
    International Journal of Environmental Research and Public Health.2020; 17(21): 7824.     CrossRef
  • Wing size and parity as markers of cohort demography for potential Anopheles (Culicidae: Diptera) malaria vectors in the Republic of Korea
    Desmond H. Foley, Richard C. Wilkerson, Heung Chul Kim, Terry A. Klein, Myung‐Soon Kim, Cong Li, David M. Levin, Leopoldo M. Rueda
    Journal of Vector Ecology.2020; 45(2): 366.     CrossRef
  • Application for Epidemiological Geographic Information System: An Open-Source Spatial Analysis Tool based on the Common Data Model (Preprint)
    Jaehyeong Cho, Seng Chan You, Seongwon Lee, DongSu Park, Bumhee Park, George Hripcsak, Rae Woong Park
    JMIR Public Health and Surveillance.2019;[Epub]     CrossRef
  • Epidemiological Characteristics of Re-emerging Vivax Malaria in the Republic of Korea (1993-2017)
    Young Yil Bahk, Hyeong-Woo Lee, Byoung-Kuk Na, Jeonga Kim, Kyoung Jin, Yeong Seon Hong, Tong-Soo Kim
    The Korean Journal of Parasitology.2018; 56(6): 531.     CrossRef
  • Genetic Diversity of Plasmodium vivax Causing Epidemic Malaria in the Republic of Korea
    Young Yil Bahk, Jeonga Kim, Seong Kyu Ahn, Byoung-Kuk Na, Jong-Yil Chai, Tong-Soo Kim
    The Korean Journal of Parasitology.2018; 56(6): 545.     CrossRef
  • Seasonal dynamics ofAnophelesspecies at three locations in the Republic of Korea
    Desmond H. Foley, Terry A. Klein, Heung Chul Kim, Myung-Soon Kim, Richard C. Wilkerson, Cong Li, Genelle Harrison, Leopoldo M. Rueda
    Journal of Vector Ecology.2017; 42(2): 335.     CrossRef
  • Assessing the impact of meteorological factors on malaria patients in demilitarized zones in Republic of Korea
    Se-Min Hwang, Seok-Joon Yoon, Yoo-Mi Jung, Geun-Yong Kwon, Soo-Nam Jo, Eun-Jeong Jang, Myoung-Ok Kwon
    Infectious Diseases of Poverty.2016;[Epub]     CrossRef
  • Vector Competence ofAnopheles kleiniandAnopheles sinensis(Diptera: Culicidae) From the Republic of Korea to Vivax Malaria-Infected Blood From Patients From Thailand
    Ratawan Ubalee, Heung-Chul Kim, Anthony L. Schuster, Patrick W. McCardle, Siriporn Phasomkusolsil, Ratree Takhampunya, Silas A. Davidson, Won-Ja Lee, Terry A. Klein
    Journal of Medical Entomology.2016; 53(6): 1425.     CrossRef
  • Age-Dependent Sex Bias in Clinical Malarial Disease in Hypoendemic Regions
    Sulabha Pathak, Mayuri Rege, Nithya J. Gogtay, Umesh Aigal, Surya Kant Sharma, Neena Valecha, Gyan Bhanot, Nilima A. Kshirsagar, Shobhona Sharma, Georges Snounou
    PLoS ONE.2012; 7(4): e35592.     CrossRef
  • Plasmodium vivax Malaria: Status in the Republic of Korea Following Reemergence
    Jae-Won Park, Gyo Jun, Joon-Sup Yeom
    The Korean Journal of Parasitology.2009; 47(Suppl): S39.     CrossRef
  • Evaluation of the Current Status of Malaria Elimination Project in the Republic of Korea and Suggestion for Improvement of Its Efficacy
    Jae-Won Park, Jee-Young Hong, Joon-Sup Yeom, Sung-Rae Cho, Dae-Kyu Oh
    Infection and Chemotherapy.2009; 41(1): 42.     CrossRef
  • Seroprevalence to the Circumsporozoite Protein Peptide Antigen of Plasmodium vivax in Korean Children
    Chae Seung Lim, Jae Kyun Yoon, Eun Ah Chang, In Bum Suh, Seong Soo A. An, Kee‐Hyoung Lee, Ji Tae Chung, Young Chang Tockgo
    Microbiology and Immunology.2005; 49(6): 521.     CrossRef
  • Hormonal and immunological mechanisms mediating sex differences in parasite infection
    S. L. Klein
    Parasite Immunology.2004; 26(6-7): 247.     CrossRef
  • Prevalence of antibodies to the circumsporozite protein of Plasmodium vivax in five different regions of Korea
    Kap No Lee, In Bum Suh, Eun Ah Chang, Soon Duck Kim, Nam Sun Cho, Phil Whan Park, Seong Soo A. An, Ok Park, Chaeseung Lim
    Tropical Medicine & International Health.2003; 8(12): 1062.     CrossRef
  • Apical membrane antigen-1 (AMA-1) gene sequences of re-emerging Plasmodium vivax in South Korea
    Eun-Taek Han, Jae-Hwan Park, Eun-Hee Shin, Min-Ho Choi, Myoung-Don Oh, Jong-Yil Chai
    The Korean Journal of Parasitology.2002; 40(3): 157.     CrossRef
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  • 83 Download
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Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis
Tae Yun Kim, Shin-Yong Kang, Il-Young Ahn, Seung-Yull Cho, Sung-Jong Hong
Korean J Parasitol 2001;39(1):57-66.
Published online March 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.1.57

In the course of immunoscreening of Clonorchis sinensis cDNA library, a cDNA CsRP12 containing a tandem repeat was isolated. The cDNA CsRP12 encodes two putative peptides of open reading frames (ORFs) 1 and 2 (CsRP12-1 and -2). The repetitive region is composed of 15 repeats of 10 amino acids. Of the two putative peptides, CsRP12-1 was proline-rich and found to have homologues in several organisms. Recombinant proteins of the putative peptides were bacterially produced and purified by an affinity chromatography. Recombinant CsRP12-1 protein was recognized by sera of clonorchiasis patients and experimental rabbits, but recombinant CsRP12-2 was not. One of the putative peptide, CsRP12-1, is designated CsPRA, proline-rich antigen of C. sinensis. Both the C-termini of CsRP12-1 and -2 were bacterially produced and analysed to show no antigenicity. Recombinant CsPRA protein showed high sensitivity and specificity. In experimental rabbits, IgG antibodies to CsPRA was produced between 4 and 8 weeks after the infection and decreased thereafter over one year. These results indicate that CsPRA is equivalent to a natural protein and a useful antigenic protein for serodiagnosis of human clonorchiasis.

Citations

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  • A point-of-care testing assay for clonorchiasis using a EuNPs-CsTR1 fluorescent probe-based immunoassay
    Xiaoxiao Ma, Huiyuan Zhang, Yiming Fang, Jing Wang, Xingyang Wang, Chen Li, Xiaolei Liu, Mingyuan Liu, Bin Tang, Yi Liu, Javier Sotillo
    PLOS Neglected Tropical Diseases.2024; 18(4): e0012107.     CrossRef
  • Evaluation of Crude and Recombinant Antigens of Schistosoma japonicum for the Detection of Schistosoma mekongi Human Infection
    Jose Ma. M. Angeles, Atcharaphan Wanlop, Minh-Anh Dang-Trinh, Masashi Kirinoki, Shin-ichiro Kawazu, Aya Yajima
    Diagnostics.2023; 13(2): 184.     CrossRef
  • Combination of Biodata Mining and Computational Modelling in Identification and Characterization of ORF1ab Polyprotein of SARS-CoV-2 Isolated from Oronasopharynx of an Iranian Patient
    Reza Zolfaghari Emameh, Hassan Nosrati, Ramezan Ali Taheri
    Biological Procedures Online.2020;[Epub]     CrossRef
  • Cs1, a Clonorchis sinensis-derived serodiagnostic antigen containing tandem repeats and a signal peptide
    Na Cheng, Xue-Nian Xu, Yan Zhou, Yu-Ting Dong, Yi-Fang Bao, Bin Xu, Wei Hu, Zheng Feng, Maria Elena Bottazzi
    PLOS Neglected Tropical Diseases.2018; 12(8): e0006683.     CrossRef
  • Heat shock proteins 70 and 90 from Clonorchis sinensis induce Th1 response and stimulate antibody production
    Eun Joo Chung, Young-Il Jeong, Myoung-Ro Lee, Yu Jung Kim, Sang-Eun Lee, Shin-Hyeong Cho, Won-Ja Lee, Mi-Yeoun Park, Jung-Won Ju
    Parasites & Vectors.2017;[Epub]     CrossRef
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    Yu-Jung Kim, Won Yoo, Myoung-Ro Lee, Jung-Mi Kang, Byoung-Kuk Na, Shin-Hyeong Cho, Mi-Yeoun Park, Jung-Won Ju
    International Journal of Molecular Sciences.2017; 18(3): 557.     CrossRef
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    Shuqi Wang, Wei Hu
    Frontiers in Microbiology.2014;[Epub]     CrossRef
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    A.M. Martínez-Ibeas, M.J. Perteguer, C. González-Lanza, T. Gárate, M.Y. Manga-González
    Experimental Parasitology.2013; 135(2): 287.     CrossRef
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    Parasitology International.2012; 61(1): 71.     CrossRef
  • Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis
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    Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
    The Korean Journal of Parasitology.2009; 47(Suppl): S59.     CrossRef
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    H.-J. Rim
    Journal of Helminthology.2005; 79(3): 269.     CrossRef
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    Acta Tropica.2003; 88(3): 195.     CrossRef
  • Use of a recombinant Clonorchis sinensis pore-forming peptide, clonorin, for serological diagnosis of clonorchiasis
    Ji-Yun Lee, Tae Yun Kim, Xiao-Xian Gan, Shin-Yong Kang, Sung-Jong Hong
    Parasitology International.2003; 52(2): 175.     CrossRef
  • Analysis of the genes expressed in Clonorchis sinensis adults using the expressed sequence tag approach
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    Parasitology Research.2003; 91(4): 283.     CrossRef
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Brief Communications

Differential expression of the 27 kDa cathepsin L-like cysteine protease in developmental stages of Spirometra erinacei
Yoon Kong, Doo-Hee Yun, Seung-Yull Cho, Woon-Mok Sohn, Young-Bae Chung, Shin-Yong Kang
Korean J Parasitol 2000;38(3):195-199.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.195

The 27 kDa cathepsin L-like cysteine protease of Spirometra erinacei plerocercoid is known to play an important function in tissue penetration, nutrient uptake and immune modulation in human sparganosis. In the present study, the expression of this enzyme was examined at different developmental stages of S. erinacei including immature egg, coracidium, plerocercoid in tadpole and rat, and adult. Proteolytic activity against carboxybenzoyl-phenylalanyl-arginyl-7-amino-4-methylcoumarin was detected in the extracts of coracidia and plerocercoid while no activity was observed in those of immature egg and adult. The specific activity in coracidial extracts was lower than that in the plerocercoid. Reverse transcription-polymerase chain reaction and Northern blot analysis demonstrated that the gene was expressed in the coracidium and plerocercoid but not in immature egg and adult. These results suggest that the 27 kDa cysteine protease is only expressed in the stages involving active migration of the parasite in the host tissue.

Citations

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  • Surgical treatment of a patient with live intracranial sparganosis for 17 years
    Jialing Hu, Kaili Liao, Xiaojin Feng, Danling Jiang, Hailin Liu, Qingcui Zheng, Hai Qiu, Fuzhou Hua, Guohai Xu, Chunhua Xu
    BMC Infectious Diseases.2022;[Epub]     CrossRef
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  • Identification of host immune regulation candidate genes of Toxascaris leonina by expression sequenced tags (ESTs) analysis
    Min Kyoung Cho, Keun Hee Lee, Sun Joo Lee, Se Won Kang, Mee Sun Ock, Yeon Chul Hong, Yong Seok Lee, Hak Sun Yu
    Veterinary Parasitology.2009; 164(2-4): 242.     CrossRef
  • Comparison of carbohydrate moieties of sparganum proteins of the snake, mouse and those of adult worm
    Hyun Jong Yang
    The Korean Journal of Parasitology.2003; 41(2): 135.     CrossRef
  • 8,857 View
  • 81 Download
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The 10 kDa protein of Taenia solium metacestodes shows genus specific antigenicity
Seung-Kyu Park, Doo-Hee Yun, Joon-Yong Chung, Yoon Kong, Seung-Yull Cho
Korean J Parasitol 2000;38(3):191-194.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.191

Genus specific antigenicity of the 10 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was demonstrated by comparative immunoblot analysis. When CFs from taeniid metacestodes of T. saginata, T. solium, T. taeniaeformis and T. crassiceps were probed with specific monoclonal antibody (mAb) raised against 150 kDa protein of T. solium metacestodes, specific antibody reactions were observed in 7 and 10 kDa proteins of T. solium and in 7/8 kDa of T. saginata, T. taeniaeformis and T. crassiceps. The mAb did not react with any protein in hydatid fluid of Echinococcus granulosus and E. multilocularis. This result revealed that the 10 kDa peptide of T. solium metacestodes and its equivalent proteins of different Taenia metacestodes are genus specific antigens that are shared among different Taenia species.

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Original Articles
IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis
Young Bae Chung, Yoon Kong, Hyun Jong Yang, Seung-Yull Cho
Korean J Parasitol 2000;38(3):145-150.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.145

Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially from experimental mice, fed 5 spargana each, were reacted with the crude extracts. Protein bands at 36-26 kDa and 103 kDa showed positive reactions since two weeks after infection. In a differential immunoblot, in which a monospecific antibody against sparganum chymase at 36 kDa was pre-treated, the reactions at 36-26 kDa disappeared, indicating that the sparganum chymase and its degradation products invoked IgG antibody reactions. When 69 patients sera of human sparganosis were examined for their IgG subclass responses, IgG4 levels showed the highest reaction which was followed by IgG1. The IgG4 antibody also reacted mainly with 36-31 kDa protease. These results indicate that 36 kDa chymase of S. mansoni plerocercoid is the main antigenic component inducing IgG antibody response in early stage of experimental sparganosis and for specific IgG subclass reactions in human sparganosis.

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    Anqi Luo, Shuyu Chen, Mingye He, Xiaoruo Tan, Zhikang Li, Wei Liu, Yisong Liu
    Vector-Borne and Zoonotic Diseases.2024;[Epub]     CrossRef
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Persisting antibody reaction in paragonimiasis after praziquantel treatment is elicited mainly by egg antigens
Seung-Yull Cho, Yoon Kong, Doo-Hee Yun, Shin-Yong Kang, Lee-Soo Kim, Young-Bae Chung, Hyun-Jong Yang
Korean J Parasitol 2000;38(2):75-84.
Published online June 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.2.75

Antibody responses in serum and cerebrospinal fluid (CSF) samples from patients with active and chronic paragonimiasis and in sera from patients on whom follow-up studies were done after praziquantel treatment were analyzed using antigens of Paragonimus westermani prepared from eggs, metacercariae, juveniles of 4- and 7-week old, adult worms and recombinant protein of 28 kDa cruzipain-like cysteine protease (rPw28CCP). The patient sera/CSFs of active and chronic paragonimiasis revealed strong antibody reactions against the crude extracts of 4- and 7-week old juveniles as well as against those from egg and adult. rPw28CCP also showed specific reaction to the sera with active paragonimiasis. After the treatment, levels of specific antibodies in the sera gradually decreased to negative range in most patients. In some cases with persisting high antibody levels, however, the reactions at 27 kDa egg protein were sustained throughout the observation period of 34 months. The reactions at 35 and 32 kDa in adult extract and rPw28CCP disappeared rapidly after the treatment. Persistent antibody reactions even after successful treatment are provoked by continuous antigenic challenge from eggs which were not resolved by treatment.

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