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Editorial Message

Journey to the next horizon
Yoon Kong
Parasites Hosts Dis 2023;61(1):1-1.
Published online February 22, 2023
DOI: https://doi.org/10.3347/PHD.00001
  • 2,016 View
  • 221 Download

Original Article

Echinococcus granulosus Protoscolex DM9 Protein Shows High Potential for Serodiagnosis of Alveolar Echinococcosis
Jeong-Geun Kim, Xiumin Han, Yoon Kong
Korean J Parasitol 2022;60(1):25-34.
Published online February 23, 2022
DOI: https://doi.org/10.3347/kjp.2022.60.1.25
Alveolar echinococcosis (AE) caused by infection with E. multilocularis metacestode, represents one of the most fatal helminthic diseases. AE is principally manifested with infiltrative, proliferating hepatic mass, resembling primary hepatocellular carcinoma. Sometimes metastatic lesions are found in nearby or remote tissue. AE diagnosis largely depends on imaging studies, but atypical findings of imaging features frequently require differential diagnosis from other hepatic lesions. Serological tests may provide further evidence, while obtaining reliable AE materials is not easy. In this study, alternative antigens, specific to AE were identified by analyzing E. granulosus protoscolex proteins. An immunoblot analysis of E. granulosus protoscolex showed that a group of low-molecular-weight proteins in the range from 14 kDa to 16 kDa exhibited a sensitive and specific immune response to AE patient sera. Partial purification and proteomic analysis indicated that this protein group contained myosin, tubulin polymerization promoting protein, fatty-acid binding protein, uncharacterized DM9, heat shock protein 90 cochaperone tebp P-23, and antigen S. When the serological applicability of recombinant forms of these proteins was assessed using enzyme-linked immunosorbent assay, DM9 protein (rEgDM9) showed 90.1% sensitivity (73/81 sera tested) and 94.5% specificity (172/181 sera tested), respectively. rEgDM9 showed weak cross-reactions with patient sera from the transitional and chronic stages of cystic echinococcosis (3 to 5 stages). rEgDM9 would serve as a useful alternative antigen for serodiagnosis of both early- and advanced-stage AE cases.
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  • 185 Download

In Memoriam

Professor Seung-Yull Cho (1943-2019)
Yoon Kong
Korean J Parasitol 2019;57(1):77-78.
Published online February 26, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.1.77

Citations

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  • Leaving behind fond memories, I am stepping away from my role as editor of the Ewha Medical Journal after finalizing this issue's theme
    Sun Huh
    Ewha Medical Journal.2025; 48(4): e51.     CrossRef
  • Presidential address: the Korean Council of Science Editors as a board member of Crossref from March 2021 to February 2024
    Sun Huh
    Science Editing.2021; 8(1): 1.     CrossRef
  • 5,121 View
  • 97 Download
  • 1 Web of Science
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Brief Communication

Evaluation of IgG4 Subclass Antibody Detection by Peptide-Based ELISA for the Diagnosis of Human Paragonimiasis Heterotrema
Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Porntip Laummaunwai, Nimit Morakote, Yoon Kong, Wanchai Maleewong
Korean J Parasitol 2013;51(6):763-766.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.763

A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values of 100%, 94.6%, 96.2%, 100%, and 88.9%, respectively. Cross reactivity was frequently seen against the sera of fascioliasis (75%) and hookworm infections (50%). Since differential diagnosis between paragonimiasis and fascioliasis can be easily done by clinical presentation and fascioliasis serology, this cross reaction is not a serious problem. Sera from patients with other parasitoses (0-25%) rarely responded to this synthetic antigen. This synthetic peptide antigen seems to be useful for development of a standardized diagnostic system for paragonimiasis.

Citations

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  • An immunochromatographic test using whole blood for rapid diagnosis of human paragonimiasis and its diagnostic usefulness
    Patcharaporn Boonroumkaew, Lakkhana Sadaow, Penchom Janwan, Rutchanee Rodpai, Oranuch Sanpool, Tongjit Thanchomnang, Hiroshi Yamasaki, Pewpan M. Intapan, Wanchai Maleewong
    Food and Waterborne Parasitology.2024; 37: e00246.     CrossRef
  • Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis
    Lakkhana Sadaow, Oranuch Sanpool, Hiroshi Yamasaki, Wanchai Maleewong, Pewpan M. Intapan
    Acta Tropica.2020; 203: 105325.     CrossRef
  • Paragonimus and paragonimiasis in Asia: An update
    Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama
    Acta Tropica.2019; 199: 105074.     CrossRef
  • A Case of Pulmonary Paragonimiasis with Chronic Abdominal Pain and Erythematous Rash in a 6-year-old Girl
    Ju Young Kim, Min Kyu Park, Yong Ju Lee, Sun Huh, Ky Young Cho
    Pediatric Infection and Vaccine.2018; 25(1): 54.     CrossRef
  • A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
    Xu Guang Qiu, Fukumi Nakamura-Uchiyama, Yukifumi Nawa, Makoto Itoh
    Tropical Medicine and Health.2016;[Epub]     CrossRef
  • Expression characteristics and specific antibody reactivity of diverse cathepsin F members of Paragonimus westermani
    Chun-Seob Ahn, Byoung-Kuk Na, Dong-ll Chung, Jeong-Geun Kim, Jin-Taek Kim, Yoon Kong
    Parasitology International.2015; 64(1): 37.     CrossRef
  • North American paragonimiasis: epidemiology and diagnostic strategies
    Peter U Fischer, Gary J Weil
    Expert Review of Anti-infective Therapy.2015; 13(6): 779.     CrossRef
  • 8,808 View
  • 109 Download
  • Crossref

Original Articles

Tetrathyridia of Mesocestoides lineatus in Chinese Snakes and Their Adults Recovered from Experimental Animals
Shin-Hyeong Cho, Tong-Soo Kim, Yoon Kong, Byoung-Kuk Na, Woon-Mok Sohn
Korean J Parasitol 2013;51(5):531-536.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.531

Morphological characteristics of Mesocestoides lineatus tetrathyridia collected from Chinese snakes and their adults recovered from experimental animals were studied. The tetrathyridia were detected mainly in the mesentery of 2 snake species, Agkistrodon saxatilis (25%) and Elaphe schrenckii (20%). They were 1.73 by 1.02 mm in average size and had an invaginated scolex with 4 suckers. Adult tapeworms were recovered from 2 hamsters and 1 dog, which were orally infected with 5-10 larvae each. Adults from hamsters were about 32 cm long and those from a dog were about 58 cm long. The scolex was 0.56 mm in average width with 4 suckers of 0.17 by 0.15 mm in average size. Mature proglottids measured 0.29 by 0.91 mm (av.). Ovaries and vitellaria bilobed and located in the posterior portion of proglottids. The cirrus sac was oval-shaped and located median. Testes were follicular, distributed in both lateral fields of proglottids, and 41-52 in number per proglottid. Gravid proglottids were 1.84 by 1.39 mm (av.) with a characteristic paruterine organ. Eggs were 35 by 27 ?m in average size with a hexacanth embryo. These morphological characteristics of adult worms were identical with those of M. lineatus reported previously. Therefore, it has been confirmed that the tetrathyridia detected in 2 species of Chinese snakes are the metacestodes of M. lineatus, and 2 snake species, A. saxatilis and E. schrenckii, play the role of intermediate hosts.

Citations

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  • Gastrointestinal parasites of cats in the Middle East (2000−2023): A literature review
    Mahdi Soroushianfar, Soheil Sadr, Alireza Sazmand, Shiva Dianaty, Javad Khedri, Rolf Karl Schuster, Hassan Borji
    Parasitology International.2024; 102: 102919.     CrossRef
  • Wood mouse (Apodemus sylvaticus L.) as intermediate host for Mesocestoides canislagopodis (Rudolphi, 1810) (Krabbe 1865) in Iceland
    Damien Jouet, Aðalsteinn Örn Snæþórsson, Karl Skírnisson
    Parasitology Research.2023; 122(9): 2119.     CrossRef
  • Squamata reptiles as a potential source of helminth infections when preyed on by companion animals
    Mariaelisa Carbonara, Jairo Alfonso Mendoza-Roldan, Riccardo Paolo Lia, Giada Annoscia, Roberta Iatta, Antonio Varcasia, Giuseppe Conte, Giovanni Benelli, Domenico Otranto
    Parasites & Vectors.2023;[Epub]     CrossRef
  • Molecular identification of Taenia hydatigena and Mesocestoides species based on copro-DNA analysis of wild carnivores in Mongolia
    Gelegbalsan Ulziijargal, Chultemsuren Yeruult, Janchiv Khulan, Choijilsuren Gantsetseg, Toni Wandra, Hiroshi Yamasaki, Myadagsuren Narankhajid
    International Journal for Parasitology: Parasites and Wildlife.2020; 11: 72.     CrossRef
  • Morphological and Molecular Characterization of Post-Larval Pre-Tetrathyridia ofMesocestoidessp. (Cestoda: Cyclophyllidea) from Ground Skink,Scincella lateralis(SAURIA: SCINCIDAE), FROM SOUTHEASTERN OKLAHOMA
    Chris T. McAllister, Vasyl V. Tkach, David Bruce Conn
    Journal of Parasitology.2018; 104(3): 246.     CrossRef
  • ПЕРВЫЕ МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКИЕ ДАННЫЕ ПО ТЕТРАТИРИДИЯМ РОДА MESOCESTOIDES ОТ КРАСНОЙ ПОЛЕВКИ ИЗ МАГАДАНСКОЙ ОБЛАСТИ, "Паразитология"
    Н. А. Поспехова, В.В. Переверзева, Н.Е. Докучаев
    Паразитология.2018; (5): 382.     CrossRef
  • Occurrence of Mesocestoides canislagopodis (Rudolphi, 1810) (Krabbe, 1865) in mammals and birds in Iceland and its molecular discrimination within the Mesocestoides species complex
    Karl Skirnisson, Damien Jouet, Hubert Ferté, Ólafur K. Nielsen
    Parasitology Research.2016; 115(7): 2597.     CrossRef
  • 12,250 View
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Experimental Murine Fascioliasis Derives Early Immune Suppression with Increased Levels of TGF-β and IL-4
Joon-Yong Chung, Young-An Bae, Doo-Hee Yun, Hyun-Jong Yang, Yoon Kong
Korean J Parasitol 2012;50(4):301-308.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.301

In fascioliasis, T-helper 2 (Th2) responses predominate, while little is known regarding early immune phenomenon. We herein analyzed early immunophenotype changes of BALB/c, C57BL/6, and C3H/He mice experimentally infected with 5 Fasciola hepatica metacercariae. A remarkable expansion of CD19+ B cells was observed as early as week 1 post-infection while CD4+/CD8+ T cells were down-regulated. Accumulation of Mac1+ cells with time after infection correlated well with splenomegaly of all mice strains tested. The expression of tumor necrosis factor (TNF)-α mRNA in splenocytes significantly decreased while that of IL-4 up-regulated. IL-1β expression was down-modulated in BALB/c and C57BL/6 mice, but not in C3H/He. Serum levels of transforming growth factor (TGF)-β were considerably elevated in all mice during 3 weeks of infection period. These collective results suggest that experimental murine fascioliasis might derive immune suppression with elevated levels of TGF-β and IL-4 during the early stages of infection.

Citations

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  • Evaluation of Th1/Th2, regulatory cytokines and transcriptional factor FoxP3 in sheep immunized with a partially protective and non-protective vaccine and challenged with Fasciola hepatica
    María Teresa Ruiz-Campillo, Isabel Lourdes Pacheco, Nieves Abril, María José Bautista, Álvaro Martínez-Moreno, Francisco Javier Martínez-Moreno, Leandro Buffoni, José Pérez, Verónica Molina-Hernández, Rafael Zafra
    Veterinary Research.2024;[Epub]     CrossRef
  • Fascioliasis: Image Findings, Diagnosis, and Treatment
    Jae Seung Lee
    Clinical Ultrasound.2024; 9(1): 18.     CrossRef
  • Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development
    Luis Miguel Flores-Velázquez, María Teresa Ruiz-Campillo, Guillem Herrera-Torres, Álvaro Martínez-Moreno, Francisco Javier Martínez-Moreno, Rafael Zafra, Leandro Buffoni, Pablo José Rufino-Moya, Verónica Molina-Hernández, José Pérez
    Frontiers in Veterinary Science.2023;[Epub]     CrossRef
  • The immunosuppression effects of deforolimus (ridaforolimus, AP23573) on allograft organ transplantation
    Lumin Wang, Yanping Li, Dawei Yang, Jiazhao Fu, Bin Zhao, Yaguang Li, Yanrong Ye, Zhongquan Qi
    Clinical and Translational Discovery.2023;[Epub]     CrossRef
  • Immunization of Goats with Recombinant Protein 14-3-3 Isoform 2(rHcftt-2) Induced Moderate Protection against Haemonchus contortus Challenge
    Yongqian Bu, Caiwen Jia, Xiaowei Tian, Kalibixiati Aimulajiang, Muhammad Ali Memon, Ruofeng Yan, Xiaokai Song, Lixin Xu, Xiangrui Li
    Pathogens.2020; 9(1): 46.     CrossRef
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    María Teresa Ruiz-Campillo, Verónica Molina-Hernández, María José Bautista, Isabel L. Pacheco, Rafael Zafra, Leandro Buffoni, Francisco Javier Martínez-Moreno, Alvaro Martínez-Moreno, José Pérez
    Veterinary Research.2020;[Epub]     CrossRef
  • Helminth infection-induced carcinogenesis: spectrometric insights from the liver flukes, Opisthorchis and Fasciola
    Maria João Gouveia, Maria Y. Pakharukova, Gabriel Rinaldi, Viatcheslav A. Mordvinov, Paul J. Brindley, Fátima Gärtner, Nuno Vale, Martin Michaelis
    Experimental Results.2020;[Epub]     CrossRef
  • Fasciola and fasciolosis in ruminants in Europe: Identifying research needs
    N. J. Beesley, C. Caminade, J. Charlier, R. J. Flynn, J. E. Hodgkinson, A. Martinez-Moreno, M. Martinez-Valladares, J. Perez, L. Rinaldi, D. J. L. Williams
    Transboundary and Emerging Diseases.2018; 65: 199.     CrossRef
  • Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection
    Isabel L. Pacheco, Nieves Abril, Rafael Zafra, Verónica Molina-Hernández, Noelia Morales-Prieto, María J. Bautista, María T. Ruiz-Campillo, Raúl Pérez-Caballero, Alvaro Martínez-Moreno, José Pérez
    Veterinary Research.2018;[Epub]     CrossRef
  • Association of Fasciola hepatica Infection with Liver Fibrosis, Cirrhosis, and Cancer: A Systematic Review
    Claudia Machicado, Jorge D. Machicado, Vicente Maco, Angelica Terashima, Luis A. Marcos, Hector H Garcia
    PLOS Neglected Tropical Diseases.2016; 10(9): e0004962.     CrossRef
  • 8,713 View
  • 68 Download
  • Crossref
Prevalence of Clonorchis sinensis Metacercariae in Freshwater Fish from Three Latitudinal Regions of the Korean Peninsula
Shin-Hyeong Cho, Woon-Mok Sohn, Byoung-Kuk Na, Tong-Soo Kim, Yoon Kong, Keeseon Eom, Won-Seok Seok, Taejoon Lee
Korean J Parasitol 2011;49(4):385-398.
Published online December 16, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.4.385

A large-scale survey was conducted to investigate the infection status of fresh water fishes with Clonorchis sinensis metacercariae (CsMc) in 3 wide regions, which were tentatively divided by latitudinal levels of the Korean peninsula. A total of 4,071 freshwater fishes were collected from 3 regions, i.e., northern (Gangwon-do: 1,543 fish), middle (Chungcheongbuk-do and Gyeongsangbuk-do: 1,167 fish), and southern areas (Jeollanam-do, Ulsan-si, and Gyeongsangnam-do: 1,361 fish). Each fish was examined by the artificial digestion method from 2003 to 2010. In northern areas, only 11 (0.7%) fish of 2 species, Pungtungia herzi and Squalidus japonicus coreanus from Hantan-gang, Cheolwon-gun, Gangwon-do were infected with av. 2.6 CsMc. In middle areas, 149 (12.8%) fish were infected with av. 164 CsMc. In southern areas, 538 (39.5%) fish were infected with av. 159 CsMc. In the analysis of endemicity in 3 regions with an index fish, P. herzi, 9 (6.2%) of 146 P. herzi from northern areas were infected with av. 2.8 CsMc. In middle areas, 34 (31.8%) of 107 P. herzi were infected with av. 215 CsMc, and in southern areas, 158 (92.9%) of 170 P. herzi were infected with av. 409 CsMc. From these results, it has been confirmed that the infection status of fish with CsMc is obviously different among the 3 latitudinal regions of the Korean peninsula with higher prevalence and burden in southern regions.

Citations

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  • Infection Characteristics of Clonorchis sinensis Metacercariae in Fish from Republic of Korea
    Woon-Mok Sohn
    The Korean Journal of Parasitology.2022; 60(2): 79.     CrossRef
  • Current status of Clonorchis sinensis and clonorchiasis in Korea: epidemiological perspectives integrating the data from human and intermediate hosts
    Won Gi Yoo, Woon-Mok Sohn, Byoung-Kuk Na
    Parasitology.2022; 149(10): 1296.     CrossRef
  • Survey of Zoonotic Trematode Metacercariae in Fish from Water Systems of Geum-gang (River) in Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Cheon-Hyeon Kim, Min-Ah Hwang, Kyeong-Woo No, Jai-Dong Kim
    The Korean Journal of Parasitology.2021; 59(1): 23.     CrossRef
  • High Endemicity with Clonorchis sinensis Metacercariae in Fish from Yongjeon-cheon (Stream) in Cheongsong-gun, Gyeongsangbuk-do, Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Hee Il Lee, Myoung-Ro Lee, Jung-Won Ju, Gou Ok Kim
    The Korean Journal of Parasitology.2021; 59(1): 97.     CrossRef
  • Prevalence and Infection Intensity of Zoonotic Trematode Metacercariae in Fish from Soyang-cheon (Stream), in Wanju-gun, Jeollabuk-do, Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Cheon-Hyeon Kim, Min-Ah Hwang, Kyeong-Woo No, Jong-Ho Park
    The Korean Journal of Parasitology.2021; 59(3): 265.     CrossRef
  • Survey of Zoonotic Trematode Metacercariae in Fish from Irrigation Canal of Togyo-jeosuji (Reservoir) in Cheorwon-gun, Gangwon-do, Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Hee Il Lee, Jung-Won Ju, Myoung-Ro Lee, Eun-Joo Lim, Sung Yong Son, Eunmi Ko, Jaeseok Choi
    The Korean Journal of Parasitology.2021; 59(4): 427.     CrossRef
  • Endemicity of Zoonotic Trematode Metacercariae in Fish from Deokcheon-gang (River) in Sancheong-gun, Gyeongsangnam-do, Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Hee Il Lee, Jung-Won Ju, Myoung-Ro Lee, Jeong-Gil Park, Jihee Ahn
    The Korean Journal of Parasitology.2021; 59(5): 523.     CrossRef
  • Prevalence of Clonorchis sinensis infection in fish in South‐East Asia: A systematic review and meta‐analysis
    Ying Zhang, Qing‐Long Gong, Qing‐Bo Lv, Yang‐Yuan Qiu, Yan‐Chun Wang, Hong‐Yu Qiu, Xin‐Rui Guo, Jun‐Feng Gao, Qiao‐Cheng Chang, Chun‐Ren Wang
    Journal of Fish Diseases.2020; 43(11): 1409.     CrossRef
  • Infection Status with Clonorchis sinensis Metacercariae in Fish from Yangcheon (Stream) in Sancheong-gun, Gyeongsangnam-do, Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju
    The Korean Journal of Parasitology.2019; 57(2): 145.     CrossRef
  • Infection Status with Digenetic Trematode Metacercariae in Fishes from Coastal Lakes in Gangwon-do, Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Soon-Won Lee
    The Korean Journal of Parasitology.2019; 57(6): 681.     CrossRef
  • Prevalence and Intensity of Clonorchis sinensis Metacercariae in Freshwater Fish from Wicheon Stream in Gunwi-gun, Gyeongsangbuk-do, Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Dong-Chul Son
    The Korean Journal of Parasitology.2018; 56(1): 41.     CrossRef
  • Infection Status with Clonorchis sinensis Metacercariae in Fish from Tamjin-gang (River) in Jeollanam-do, Republic of Korea
    Ki-Bok Yoon, Hyun-Cheol Lim, Doo Young Jeon, Sook Park, Shin-Hyeong Cho, Jung-Won Ju, Sung-Shik Shin, Byoung-Kuk Na, Woon-Mok Sohn
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  • Infections with Digenean Trematode Metacercariae in Two Invasive Alien Fish, Micropterus salmoides and Lepomis macrochirus, in Two Rivers in Chungcheongbuk-do, Republic of Korea
    Seongjun Choe, Hansol Park, Dongmin Lee, Yeseul Kang, Hyeong-Kyu Jeon, Keeseon S. Eom
    The Korean Journal of Parasitology.2018; 56(5): 509.     CrossRef
  • A new method for estimating the prevalence of clonorchiasis in Korea
    Jin-Young Jeong, Jin Yong Lee, Byung-Suk Chung, Younghyun Choi, Allison Baer Alley, Hyun Joo Kim
    Medicine.2017; 96(13): e6536.     CrossRef
  • Prevalence of Clonorchis sinensis Metacercariae in Fish from Water Systems of Seomjin-gang (River)
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Mi-Yeoun Park, Cheon-Hyeon Kim, Min-Ah Hwang, Kyeong-Woo No, Ki-Bok Yoon, Hyun-Cheol Lim
    The Korean Journal of Parasitology.2017; 55(3): 305.     CrossRef
  • Trematode Metacercariae in Freshwater Fish from Water Systems of Hantangang and Imjingang in Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Soon-Won Lee, Seung-Bong Choi, Won-Seok Seok
    The Korean Journal of Parasitology.2015; 53(3): 289.     CrossRef
  • Prevalence of Zoonotic Trematode Metacercariae in Freshwater Fish from Gangwon-do, Korea
    Shin-Hyeong Cho, Won-Ja Lee, Tong-Soo Kim, Won-Seok Seok, Taejoon Lee, Kyungjin Jeong, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2014; 52(4): 399.     CrossRef
  • Prevalence and Risk Factors of Clonorchiasis among Residents of Riverside Areas in Muju-gun, Jeollabuk-do, Korea
    Do-Soon Park, Sung-Jin Na, Shin Hyeong Cho, Kyung Ja June, Young-Chae Cho, Young-Ha Lee
    The Korean Journal of Parasitology.2014; 52(4): 391.     CrossRef
  • 10,199 View
  • 98 Download
  • Crossref

Mini Review

Successful Control of Lymphatic Filariasis in the Republic of Korea
Hyeng-Il Cheun, Yoon Kong, Shin-Hyeong Cho, Jong-Soo Lee, Jong-Yil Chai, Joo-Shil Lee, Jong-Koo Lee, Tong-Soo Kim
Korean J Parasitol 2009;47(4):323-335.
Published online December 1, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.4.323

A successful experience of lymphatic filariasis control in the Republic of Korea is briefly reviewed. Filariasis in the Republic of Korea was exclusively caused by infection with Brugia malayi. Over the past several decades from the 1950s to 2006, many investigators exerted their efforts to detection, treatment, and follow-up of filariasis patients in endemic areas, and to control filariasis. Mass, combined with selective, treatments with diethylcarbamazine to microfilaria positive persons had been made them free from microfilaremia and contributed to significant decrease of the microfilarial density in previously endemic areas. Significant decrease of microfilaria positive cases in an area influenced eventually to the endemicity of filariasis in the relevant locality. Together with remarkable economic growth followed by improvement of environmental and personal hygiene and living standards, the factors stated above have contributed to blocking the transmission cycle of B. malayi and led to disappearance of this mosquito-borne ancient disease in the Republic of Korea.

Citations

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  • Current Status and a Perspective of Mosquito-Borne Diseases in the Republic of Korea
    Jae Hyoung Im, Tong-Soo Kim, Moon-Hyun Chung, Ji Hyeon Baek, Hea Yoon Kwon, Jin-Soo Lee
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Original Articles

A Nationwide Survey on the Prevalence of Intestinal Parasitic Infections in the Republic of Korea, 2004
Tong-Soo Kim, Shin-Hyeong Cho, Sun Huh, Yoon Kong, Woon-Mok Sohn, Seung-Sik Hwang, Jong-Yil Chai, Soon-Hyung Lee, Yun-Kyu Park, Dae-Kyu Oh, Jong-Koo Lee
Korean J Parasitol 2009;47(1):37-47.
Published online March 12, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.1.37

National surveys on the prevalence of intestinal parasitic infections have been carried out every 5-7 years since 1971 in the Republic of Korea in order to establish control measures. The present nationwide survey was conducted from June to December 2004. The 10% population sampling data of Population and Housing Census by the Korean government in 2000 was used as the survey population. One sample was selected randomly from each of the 22,858 registered subjects, and a total of 20,541 people were ultimately included in this survey. Fecal examinations were performed by the cellophane thick smear and saturated brine flotation techniques. Pinworm infection was examined by cello-tape anal swab method. This survey also included a questionnaire study for a socioeconomic analysis. The total helminth egg positive rate was 3.7%, and the estimated total positive number among nationwide people was 1,780,000. The rates in urban and rural areas were 3.1% and 6.8%, respectively. As the total egg positive rate in the 6th survey in 1997 was 2.4%, the present survey showed that there was a considerable degree of increase in the prevalence rate of intestinal parasitic infections over the 7-year period following the 6th survey. The largest increases occurred in the egg positive rates of Clonorchis sinensis and heterophyids including Metagonimus yokogawai.

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Larval Gnathostoma hispidum detected in the red banded odd-tooth snake, Dinodon rufozonatum rufozonatum, from China
Shin-Hyeong Cho, Tong-Soo Kim, Yoon Kong, Byoung-Kuk Na, Woon-Mok Sohn
Korean J Parasitol 2007;45(3):191-198.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.191

A total of 205 larval gnathostomes were collected from 18 (22.5%) of 80 red banded odd-tooth snakes, Dinodon rufozonatum rufozonatum, which had been smuggled from China and confiscated at Customs in Busan, Republic of Korea. In order to identify the species, some of the larvae were observed by a light microscope and a scanning electron microscope (SEM). The larvae were 2.18 x 0.29 mm in average size, and had a pair of lips at the anterior end, a muscular esophagus, 2 pairs of cervical sacs, and brownish intestines. The head bulb was characteristically equipped with 4 rows of hooklets; the average number of hooklets in each respective row was 38.6, 40.5, 41.5, and 43.7. In SEM views, the mouth evidenced a pair of lateral lips of equal size in a half-moon shape. Each lip featured a couple of labial papillae and a small amphid located between the 2 papillae. The hooklets on the head bulb had single-pointed, posteriorly-curved tips. The cuticular spines were larger and more densely distributed on the anterior part of the body, and decreased gradually in size and number toward the posterior body. On the basis of these morphological characteristics, the larvae were identified as the third stage larvae of Gnathostoma hispidum.

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    Gilang Maulana Putra, Emmanuel Djoko Poetranto, Fedik Abdul Rantam
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    Shin-Hyeong Cho, Tong-Soo Kim, Yoon Kong, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2013; 51(5): 531.     CrossRef
  • The Jeju Weasel,Mustela sibilica quelpartis, A New Definitive Host forGnathostoma nipponicumYamaguti, 1941
    Ho-Choon Woo, Hong-Shik Oh, Shin-Hyeong Cho, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2011; 49(3): 317.     CrossRef
  • Discovery of LarvalGnathostoma nipponicumin Frogs and Snakes from Jeju-do (Province), Republic of Korea
    Ho-Choon Woo, Hong-Shik Oh, Shin-Hyeong Cho, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2011; 49(4): 445.     CrossRef
  • Precocity of Gnathostoma turgidum in naturally infected four-eyed opossum Philander opossum pallidus from Temascal, Oaxaca, Mexico
    Roberto Javier Almeyda-Artigas, Miguel Ángel Mosqueda-Cabrera, Edmundo Sánchez-Núñez
    Parasitology Research.2010; 106(2): 439.     CrossRef
  • Detection of Gnathostoma spinigerum Third-Stage Larvae in Snakeheads Purchased from a Central Part of Myanmar
    Bong-Kwang Jung, Jin-Ju Lee, Kyoung-Ho Pyo, Hyeong-Jin Kim, Hoo-Gn Jeong, Cheong-Ha Yoon, Soon-Hyung Lee, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2008; 46(4): 285.     CrossRef
  • 13,171 View
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Brief Communication

Infection status of pond smelts, Hypomesus olidus, and other freshwater fishes with trematode metacercariae in 6 large lakes
Shin-Hyeong Cho, Woon-Mok Sohn, Sung-Shik Shin, Hyeon-Je Song, Taek-Gyun Choi, Chang-Mi Oh, Yoon Kong, Tong-Soo Kim
Korean J Parasitol 2006;44(3):243-246.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.243

In order to determine the current infection status of pond smelts, Hypomesus olidus, and other freshwater fishes with trematode metacercariae, a total of 4,861 pond smelts and 18 other freshwater fishes, collected from the Soyang and Uiam Lakes in Gangwon-do, the Unam Lake in Jeollabuk-do, the Jangseong Lake in Jeollanam-do, the Uirim-ji (lake) in Chungcheongbuk-do, and the Andong Lake in Gyeongsangbuk-do, were individually digested with 1% pepsin-HCl and examined under a dissecting microscope. In all pond smelts caught from the 6 lakes, we were unable to detect any known human infectious trematode metacercariae in Korea. However, in other freshwater fishes, such as, Squalidus japonicus coreanus (Unam Lake), and Zacco platypus (Jangseong Lake) and Opsariichthys uncirostris amurensis (Jangseong Lake), metacercariae of human-infecting trematodes, i.e., Clonorchis sinensis and Metagonimus sp. were detected, respectively.

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  • Ultrasound-Assisted Enhancement of Gel Properties in Hypomesus olidus Surimi
    Yuan Fu, Guochuan Jiang, Xing Sun, Shuibing Yang, Jiahang Yu, Xuejun Liu, Liyan Wang, Shuangjie Zhu
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  • Physicochemical and functional properties of the muscle protein fraction of Hypomesus olidus
    Yuan Fu, Chuanhao Liu, Xiaohui Yan, Guochuan Jiang, Qiao Dang, Liyan Wang, Xuejun Liu
    Food Chemistry: X.2022; 16: 100484.     CrossRef
  • Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus
    Xuan Zhang, Xin‐Tong Ma, Yu Xu, Guo‐Chuan Jiang, Jia‐Lin Zhang, Xue‐Jun Liu, Xiao‐Hui Yan
    Food Science & Nutrition.2020; 8(2): 849.     CrossRef
  • Intestinal Flukes Recovered from a Herring Gull, Larus argentatus, in the Republic of Korea
    Young-Il Lee, Min Seo, Jong-Yil Chai
    The Korean Journal of Parasitology.2020; 58(1): 81.     CrossRef
  • Monitoring of Lake area Change and Drought using Landsat Images and the Artificial Neural Network Method in Lake Soyang, Chuncheon, Korea
    Jinah Eom, Sungjae Park, Bokyun Ko, Chang-Wook Lee
    Journal of the Korean earth science society.2020; 41(2): 129.     CrossRef
  • Prevalence of Clonorchis sinensis infection in freshwater fishes in northeastern China
    Y. Zhang, Q.C. Chang, Y. Zhang, L. Na, W.T. Wang, W.W. Xu, D.Z. Gao, Z.X. Liu, C.R. Wang, X.Q. Zhu
    Veterinary Parasitology.2014; 204(3-4): 209.     CrossRef
  • Prevalence ofClonorchis sinensisMetacercariae in Freshwater Fish from Three Latitudinal Regions of the Korean Peninsula
    Shin-Hyeong Cho, Woon-Mok Sohn, Byoung-Kuk Na, Tong-Soo Kim, Yoon Kong, Keeseon Eom, Won-Seok Seok, Taejoon Lee
    The Korean Journal of Parasitology.2011; 49(4): 385.     CrossRef
  • Infection Status of Freshwater Fish with Metacercariae of Clonorchis sinensis in Korea
    Eun-Min Kim, Jae-Lip Kim, Sung Yil Choi, Jae-Whan Kim, Siwon Kim, Min-Ho Choi, Young Mee Bae, Soon-Hyung Lee, Sung-Tae Hong
    The Korean Journal of Parasitology.2008; 46(4): 247.     CrossRef
  • Survey for zoonotic liver and intestinal trematode metacercariae in cultured and wild fish in An Giang Province, Vietnam
    Nguyen Diem Thu, Anders Dalsgaard, Ly Thi Thanh Loan, K. Darwin Murrell
    The Korean Journal of Parasitology.2007; 45(1): 45.     CrossRef
  • 8,324 View
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Original Articles

Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection
Hyeong-Woo Lee, Sung-Ung Moon, Hye-Sun Ryu, Yeon-Joo Kim, Shin-Hyeong Cho, Gyung-Tae Chung, Khin Lin, Byoung-Kuk Na, Yoon Kong, Kyung-Suk Chung, Tong-Soo Kim
Korean J Parasitol 2006;44(1):49-54.
Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.49

In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was 39.1% (47/120) in thin smear trials, and 33.3% (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 (30.8%) was lower than that of the blood stage antigens (70.8%), rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases (10.0%), in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.

Citations

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  • Development of new real-time PCR assays for detection and species differentiation of Plasmodium ovale
    Wenqiao He, Rachel Sendor, Varun R. Potlapalli, Melchior M. Kashamuka, Antoinette K. Tshefu, Fernandine Phanzu, Albert Kalonji, Billy Ngasala, Kyaw Lay Thwai, Jonathan J. Juliano, Jessica T. Lin, Jonathan B. Parr, Georges Snounou
    PLOS Neglected Tropical Diseases.2024; 18(9): e0011759.     CrossRef
  • First characterization of Plasmodium vivax liver stage antigen (PvLSA) using synthetic peptides
    Youn-Kyoung Goo, Eun-Jeong Seo, Yeon-kyung Choi, Hyun-Il Shin, Jetsumon Sattabongkot, So-Young Ji, Chom-Kyu Chong, Shin-Hyung Cho, Won-Ja Lee, Jung-Yeon Kim
    Parasites & Vectors.2014;[Epub]     CrossRef
  • Comparison of the antibody responses to Plasmodium vivax and Plasmodium falciparum antigens in residents of Mandalay, Myanmar
    Tong-Soo Kim, Hyung-Hwan Kim, Jung-Yeon Kim, Yoon Kong, Byoung-Kuk Na, Khin Lin, Sung-Ung Moon, Yeon-Joo Kim, Myoung-Hee Kwon, Youngjoo Sohn, Hyuck Kim, Hyeong-Woo Lee
    Malaria Journal.2011;[Epub]     CrossRef
  • Assessment of exposure to Plasmodium falciparum transmission in a low endemicity area by using multiplex fluorescent microsphere-based serological assays
    Jean Biram Sarr, Eve Orlandi-Pradines, Sonia Fortin, Cheikh Sow, Sylvie Cornelie, François Rogerie, Soihibou Guindo, Lassana Konate, Thierry Fusaï, Gilles Riveau, Christophe Rogier, Franck Remoue
    Parasites & Vectors.2011;[Epub]     CrossRef
  • The malaria candidate vaccine liver stage antigen-3 is highly conserved in Plasmodium falciparum isolates from diverse geographical areas
    Eric Prieur, Pierre Druilhe
    Malaria Journal.2009;[Epub]     CrossRef
  • High Levels of Antibodies to Plasmodium falciparum Liver Stage Antigen-1 in Naturally Infected Individuals in Myanmar
    Hyeong-Woo Lee, Sung-Ung Moon, Yeon-Joo Kim, Shin-Hyeong Cho, Khin Lin, Byoung-Kuk Na, Tong-Soo Kim
    The Korean Journal of Parasitology.2008; 46(3): 195.     CrossRef
  • Liver stage antigen 3 isolated from a cDNA library of Plasmodium falciparum erythrocytic stages
    Eva M. Moyano, Luis Miguel González, Susana Arahuetes, Agustín Benito
    Parasitology Research.2007; 102(1): 111.     CrossRef
  • 8,482 View
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A seroepidemiological survey of Taenia solium cysticercosis in Nabo, Guangxi Zhuang Autonomous Region, China
Joon-Yong Chung, Keeseon S. Eom, Yichao Yang, Xenming Li, Zheng Feng, Han-Jong Rim, Seung-Yull Cho, Yoon Kong
Korean J Parasitol 2005;43(4):135-139.
Published online December 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.4.135

We have observed the seropositive rate of Taenia solium cysticercosis in residents at Nabo Village, Tiandong County, Guangxi Zhuang Autonomous Region, China by enzyme linked immunosorbent assay. The village had been found to be a relatively high endemic area of porcine cysticercosis among roaming pigs. Of 202 persons examined four males aged 15, 25, 35 and 41 year-old exhibited absorbance (abs) at 0.18, 0.20, 0.35 and 0.55, respectively. In addition, two females whose ages were 35 and 39 years revealed specific antibody levels of abs 0.26 and 0.41 in their sera. Overall positive rate among the people was 2.97%. All of these persons agreed that they had ingested the pork infected with T. solium metacestode (TsM), while history of proglottid discharge was not noticed from all of them. Three males and one female complained of intermittent headache. Our findings reinforced not only that the prevalence of cysticercosis might be related with roaming pigs infected with TsM but also that behavioral and environmental practices in local community constituted risk factors for transmission of the infection.

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  • A Marked Decline in Taenia solium Taeniasis and Cysticercosis Infections in China: Possible Reasons from the Ecological Determinants Perspective
    Junqiang Li, Feifei Xu, Yuancai Chen, Md Robiul Karim, Yilin Wang, Kaihui Zhang, Xiaoying Li, Pitambar Dhakal, Rongjun Wang, Longxian Zhang
    Zoonoses.2023;[Epub]     CrossRef
  • The burden of T. solium cysticercosis and selected neuropsychiatric disorders in Mocuba district, Zambézia province, Mozambique
    Irene Langa, Fernando Padama, Noémia Nhancupe, Alberto Pondja, Delfina Hlashwayo, Lidia Gouveia, Dominik Stelzle, Clarissa Prazeres da Costa, Veronika Schmidt, Andrea S. Winkler, Emília Virgínia Noormahomed, Eduardo Torres
    PLOS Neglected Tropical Diseases.2022; 16(7): e0010606.     CrossRef
  • Taeniasis and cysticercosis in Asia: A review with emphasis on molecular approaches and local lifestyles
    Akira Ito, Tiaoying Li, Toni Wandra, Paron Dekumyoy, Tetsuya Yanagida, Munehiro Okamoto, Christine M Budke
    Acta Tropica.2019; 198: 105075.     CrossRef
  • Infections of Soil-Transmitted Helminth in Refugees from North Korea
    Young-Il Lee, Min Seo, Suk-Bae Kim
    The Korean Journal of Parasitology.2018; 56(3): 291.     CrossRef
  • Zoonoses in South-East Asia: a regional burden, a global threat
    Marion Bordier, François Roger
    Animal Health Research Reviews.2013; 14(1): 40.     CrossRef
  • Prevalence and Risk Factors Associated with Human Taenia Solium Infections in Mbozi District, Mbeya Region, Tanzania
    Gloria Mwanjali, Charles Kihamia, Deodatus Vitalis Conatus Kakoko, Faustin Lekule, Helena Ngowi, Maria Vang Johansen, Stig Milan Thamsborg, Arve Lee Willingham, Malcolm K. Jones
    PLoS Neglected Tropical Diseases.2013; 7(3): e2102.     CrossRef
  • Serodiagnostic reliability of single-step enriched low-molecular weight proteins of Taenia solium metacestode of American and Asian isolates
    E.-G. Lee, Y.-A. Bae, S.-H. Kim, S.P. Díaz-Camacho, Y. Nawa, Y. Kong
    Transactions of the Royal Society of Tropical Medicine and Hygiene.2010; 104(10): 676.     CrossRef
  • Seroprevalence of Taenia solium antibodies in Vellore district, south India
    V. Prabhakaran, M. Venkata Raghava, V. Rajshekhar, J. Muliyil, A. Oommen
    Transactions of the Royal Society of Tropical Medicine and Hygiene.2008; 102(3): 246.     CrossRef
  • What triggers seizures in neurocysticercosis? A MRI-based study in pig farming community from a district of North India
    Amit Prasad, Rakesh K. Gupta, Sunil Pradhan, Mukesh Tripathi, Chandra M. Pandey, Kashi N. Prasad
    Parasitology International.2008; 57(2): 166.     CrossRef
  • Tissue parasitic helminthiases are prevalent at Cheongjin, North Korea
    Chenghua Shen, Shunyu Li, Shanzi Zheng, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
    The Korean Journal of Parasitology.2007; 45(2): 139.     CrossRef
  • 8,619 View
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Aspartic proteases of Plasmodium vivax are highly conserved in wild isolates
Byoung-Kuk Na, Eung-Goo Lee, Hyeong-Woo Lee, Shin-Hyeong Cho, Young-An Bae, Yoon Kong, Jong-Koo Lee, Tong-Soo Kim
Korean J Parasitol 2004;42(2):61-66.
Published online June 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.2.61

The plasmepsins are the aspartic proteases of malaria parasites. Treatment of aspartic protease inhibitor inhibits hemoglobin hydrolysis and blocks the parasite development in vitro suggesting that these proteases might be exploited their potentials as antimalarial drug targets. In this study, we determined the genetic variations of the aspartic proteases of Plasmodium vivax (PvPMs) of wild isolates. Two plasmepsins (PvPM4 and PvPM5) were cloned and sequenced from 20 P. vivax Korean isolates and two imported isolates. The sequences of the enzymes were highly conserved except a small number of amino acid substitutions did not modify key residues for the function or the structure of the enzymes. The high sequence conservations between the plasmepsins from the isolates support the notion that the enzymes could be reliable targets for new antimalarial chemotherapeutics.

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  • The Effect of Aqueous Extract of Cinnamon on the Metabolome ofPlasmodium falciparumUsing1HNMR Spectroscopy
    Shirin Parvazi, Sedigheh Sadeghi, Mehri Azadi, Maryam Mohammadi, Mohammad Arjmand, Farideh Vahabi, Somye Sadeghzadeh, Zahra Zamani
    Journal of Tropical Medicine.2016; 2016: 1.     CrossRef
  • Use of multiplex real-time PCR for detection of common diarrhea causing protozoan parasites in Egypt
    John T. Nazeer, Khalifa El Sayed Khalifa, Heidrun von Thien, Mahmoud Mohamed El-Sibaei, Magda Youssef Abdel-Hamid, Ranya Ayman Samir Tawfik, Egbert Tannich
    Parasitology Research.2013; 112(2): 595.     CrossRef
  • Imperfect Duplicate Insertions Type of Mutations in Plasmepsin V Modulates Binding Properties of PEXEL Motifs of Export Proteins in Indian Plasmodium vivax
    Manmeet Rawat, Sonam Vijay, Yash Gupta, Pramod Kumar Tiwari, Arun Sharma, Rajvir Dahiya
    PLoS ONE.2013; 8(3): e60077.     CrossRef
  • Sequence homology and structural analysis of plasmepsin 4 isolated from Indian Plasmodium vivax isolates
    Manmeet Rawat, Sonam Vijay, Yash Gupta, Rajnikant Dixit, P.K. Tiwari, Arun Sharma
    Infection, Genetics and Evolution.2011; 11(5): 924.     CrossRef
  • Single nucleotide polymorphisms, putatively neutral DNA markers and population genetic parameters in IndianPlasmodium vivaxisolates
    BHAVNA GUPTA, ADITYA P. DASH, NALINI SHRIVASTAVA, APARUP DAS
    Parasitology.2010; 137(12): 1721.     CrossRef
  • Characterization of plasmepsin V, a membrane-bound aspartic protease homolog in the endoplasmic reticulum of Plasmodium falciparum
    Michael Klemba, Daniel E. Goldberg
    Molecular and Biochemical Parasitology.2005; 143(2): 183.     CrossRef
  • Purification and Characterization of a Hemoglobin Degrading Aspartic Protease from the Malarial Parasite Plasmodium vivax
    Arun Sharma, Alex Eapen, Sarala K. Subbarao
    The Journal of Biochemistry.2005; 138(1): 71.     CrossRef
  • 8,687 View
  • 69 Download
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A highly specific antigenic protein of 31 kDa from plerocercoid of Spirometra mansoni (sparganum) was obtained by gelatin affinity and Mono Q anion-exchange column chromatography. The purified 31 kDa protein was subjected to N-glycan enzymatic digestion for structural analysis. The relative electrophoretic mobility was analyzed by SDS-PAGE, before and after digestion. On SDS-PAGE after enzymatic digestion, the 31 kDa protein showed a molecular shift of approximately 2 kDa, which indicated the possession of complex N-linked oligosaccharides (N-glycosidase F sensitive) but not of high-mannose oligosaccharides (endo-beta-N-acetylglucosaminidase H, non-sensitive). Chemically periodated 31 kDa protein showed statistically non-significant changes with human sparganosis sera by enzyme linked immunosorbent assay (ELISA). Therefore, the dominant epitopes of the 31 kDa molecule in human sparganosis were found to be mainly polypeptide, while N-glycans of the antigenic molecule in sparganum was minimal in anti-carbohydrate antibody production.

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  • Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity
    Yanina Arana, Manuela Verastegui, Iskra Tuero, Louis Grandjean, Hector H. Garcia, Robert H. Gilman
    Parasitology Research.2013; 112(10): 3569.     CrossRef
  • 7,894 View
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Divergent long-terminal-repeat retrotransposon families in the genome of Paragonimus westermani
Young-An Bae, Yoon Kong
Korean J Parasitol 2003;41(4):221-231.
Published online December 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.4.221

To gain information on retrotransposons in the genome of Paragonimus westermani, PCR was carried out with degenerate primers, specific to protease and reverse transcriptase (rt) genes of long-terminal-repeat (LTR) retrotransposons. The PCR products were cloned and sequenced, after which 12 different retrotransposon-related sequences were isolated from the trematode genome. These showed various degrees of identity to the polyprotein of divergent retrotransposon families. A phylogenetic analysis demonstrated that these sequences could be classified into three different families of LTR retrotransposons, namely, Xena, Bel, and Gypsy families. Of these, two mRNA transcripts were detected by reverse transcriptase-PCR, showing that these two elements preserved their mobile activities. The genomic distributions of these two sequences were found to be highly repetitive. These results suggest that there are diverse retrotransposons including the ancient Xena family in the genome of P. westermani, which may have been involved in the evolution of the host genome.

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  • Gene diversity and genetic variation in lung flukes (genusParagonimus)
    David Blair, Yukifumi Nawa, Makedonka Mitreva, Pham Ngoc Doanh
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 6.     CrossRef
  • Transfection of Platyhelminthes
    Bárbara Moguel, Raúl J. Bobes, Julio C. Carrero, Juan P. Laclette
    BioMed Research International.2015; 2015: 1.     CrossRef
  • Transient transgenesis of the tapeworm Taenia crassiceps
    Bárbara Moguel, Norma Moreno-Mendoza, Raúl J. Bobes, Julio C. Carrero, Jesús Chimal-Monroy, Martha E. Díaz-Hernández, Luis Herrera-Estrella, Juan P. Laclette
    SpringerPlus.2015;[Epub]     CrossRef
  • Review Article: ISTR, a Retrotransposons-Based Marker to Assess Plant Genome Variability with Special Emphasis in the Genera <i>Zea</i> and <i>Agave</i>
    Martha Isabel Torres-Morán, Norma Almaraz-Abarca, Martha Escoto-Delgadillo
    American Journal of Plant Sciences.2012; 03(12): 1820.     CrossRef
  • PwRn1, a novel Ty3/gypsy-like retrotransposon of Paragonimus westermani: molecular characters and its differentially preserved mobile potential according to host chromosomal polyploidy
    Young-An Bae, Jong-Sook Ahn, Seon-Hee Kim, Mun-Gan Rhyu, Yoon Kong, Seung-Yull Cho
    BMC Genomics.2008;[Epub]     CrossRef
  • Both sense and antisense strands of the LTR of the Schistosoma mansoni Pao-like retrotransposon Sinbad drive luciferase expression
    Claudia S. Copeland, Victoria H. Mann, Paul J. Brindley
    Molecular Genetics and Genomics.2007; 277(2): 161.     CrossRef
  • 9,014 View
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The evolutionary course of the CsRn1 long-terminal-repeat (LTR) retrotransposon was predicted by conducting a phylogenetic analysis with its paralog LTR sequences. Based on the clustering patterns in the phylogenetic tree, multiple CsRn1 copies could be grouped into four subsets, which were shown to have different integration times. Their differential sequence divergences and heterogeneous integration patterns strongly suggested that these subsets appeared sequentially in the genome of C. sinensis. Members of recently expanding subset showed the lowest level of divergence in their LTR and reverse transcriptase gene sequences. They were also shown to be highly polymorphic among individual genomes of the trematode. The CsRn1 element exhibited a preference for repetitive, agenic chromosomal regions in terms of selecting integration targets. Our results suggested that CsRn1 might induce a considerable degree of intergenomic variation and, thereby, have influenced the evolution of the C. sinensis genome.

Citations

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  • Recurrent emergence of structural variants of LTR retrotransposon CsRn1 evolving novel expression strategy and their selective expansion in a carcinogenic liver fluke, Clonorchis sinensis
    Seon-Hee Kim, Yoon Kong, Young-An Bae
    Molecular and Biochemical Parasitology.2017; 214: 14.     CrossRef
  • Genomics and transcriptomics of the Chinese liver fluke Clonorchis sinensis (Opisthorchiidae, Trematoda)
    G. N. Chelomina
    Molecular Biology.2017; 51(2): 184.     CrossRef
  • Egg-specific expression of protein with DNA methyltransferase activity in the biocarcinogenic liver fluke Clonorchis sinensis
    SEON-HEE KIM, HYE-JEONG CHO, WOON-MOK SOHN, CHUN-SEOB AHN, YOON KONG, HYUN-JONG YANG, YOUNG-AN BAE
    Parasitology.2015; 142(9): 1228.     CrossRef
  • Progress on the transcriptomics of carcinogenic liver flukes of humans—Unique biological and biotechnological prospects
    Neil D. Young, Aaron R. Jex, Cinzia Cantacessi, Bronwyn E. Campbell, Thewarach Laha, Woon-Mok Sohn, Banchob Sripa, Alex Loukas, Paul J. Brindley, Robin B. Gasser
    Biotechnology Advances.2010; 28(6): 859.     CrossRef
  • The biology and evolution of transposable elements in parasites
    M. Carmen Thomas, Francisco Macias, Carlos Alonso, Manuel C. López
    Trends in Parasitology.2010; 26(7): 350.     CrossRef
  • Functional Genes and Proteins of Clonorchis sinensis
    Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
    The Korean Journal of Parasitology.2009; 47(Suppl): S59.     CrossRef
  • Molecular genetic profiles among individual Clonorchis sinensis adults collected from cats in two geographic regions of China revealed by RAPD and MGE-PCR methods
    De-Hua Lai, Qiao-Ping Wang, Wen Chen, Lian-Shun Cai, Zhong-Dao Wu, Xing-Quan Zhu, Zhao-Rong Lun
    Acta Tropica.2008; 107(2): 213.     CrossRef
  • PwRn1, a novel Ty3/gypsy-like retrotransposon of Paragonimus westermani: molecular characters and its differentially preserved mobile potential according to host chromosomal polyploidy
    Young-An Bae, Jong-Sook Ahn, Seon-Hee Kim, Mun-Gan Rhyu, Yoon Kong, Seung-Yull Cho
    BMC Genomics.2008;[Epub]     CrossRef
  • 8,015 View
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A seroepidemiological survey for toxocariasis in apparently healthy residents in Gangwon-do, Korea
Hyun-Young Park, Soo-Ung Lee, Sun Huh, Yoon Kong, Jean-Fran?ois Magnaval
Korean J Parasitol 2002;40(3):113-117.
Published online September 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.3.113

We investigated the sero-prevalence of toxocariasis among healthy Korean adults in 1999. A total of 314 sera from normal inhabitants in Whachon-gun, Gangwon-do, Korea was examined for specific antibody levels against excretory-secretory products of second stage larvae of Toxocara (TES). The presence of cross-reactions with other helminthiases such as cysticercosis, paragonimiasis, sparganosis or clonorchiasis was also checked by specific IgG ELISA. Sera showing positive reaction against TES were also tested by IgG immunoblot and by IgE ELISA. Out of 314 subjects, 16 was found to be positive by TES IgG ELISA and immunoblot, among whom 12 were also positive by TES IgE ELISA. Among the 16 seropositive samples, two sera showed positive reaction against Paragonimus and sparganum antigen, respectively. These results inferred that cross-reactions were negligible between toxocariasis and other helminthiases. Toxocariasis seroprevalence among Korean rural adults was detected to be approximately 5%.

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    Yeji Lee, Young seop Song, Ji hoon Phi, In-one Kim, Keewon Kim
    Child's Nervous System.2023; 39(3): 811.     CrossRef
  • TOXOCARA CANİS VE VİSCERAL LARVA MİGRANS
    Selma KOCADEMİR, Kader YILDIZ
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  • Prevalence of Toxocariasis and Its Risk Factors in Patients with Eosinophilia in Korea
    Hyun Beom Song, Deokho Lee, Yan Jin, Jinwoo Kang, Shin-Hyeong Cho, Min Sun Park, Jin-Ho Park, Woo-Jung Song, Hye-Ryun Kang, Sang Hyub Lee, Sung-Tae Hong, Min-Ho Choi
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Excretory bladder: the source of cysteine proteases in Paragonimus westermani metacercariae
Hyun-Jong Yang, Young-Bae Chung, Shin-Yong Kang, Yoon Kong, Seung-Yull Cho
Korean J Parasitol 2002;40(2):89-92.
Published online June 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.2.89

The cysteine proteases of Paragonimus westermani metacercariae are involved in metacercarial excystment, host immune modulation, and possibly in tissue penetration. In order to clarify the origin of the enzymes, 28 and 27 kDa cysteine proteases in metacercarial excretory-secretory products were purified through the FPLC system using Mono Q column chromatography. The polyclonal antibodies to the enzymes were produced in BALB/c mice. Immunolocalization studies revealed that both cysteine proteases were distributed at the linings of excretory bladder and excretory concretions of the metacercariae. It was suggested that the excretory epithelium of P. westermani undertake the secretory function of metacercarial cysteine proteases, in addition to its role as a route for eliminating waste products.

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  • Early Cysteine Protease Activity in Excretory Bladder Triggers Metacercaria Excystment of Paragonimus westermani
    Y. B. Chung, T. S. Kim, H. J. Yang
    Journal of Parasitology.2005; 91(4): 953.     CrossRef
  • 7,669 View
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Brief Communications

Differential expression of the 27 kDa cathepsin L-like cysteine protease in developmental stages of Spirometra erinacei
Yoon Kong, Doo-Hee Yun, Seung-Yull Cho, Woon-Mok Sohn, Young-Bae Chung, Shin-Yong Kang
Korean J Parasitol 2000;38(3):195-199.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.195

The 27 kDa cathepsin L-like cysteine protease of Spirometra erinacei plerocercoid is known to play an important function in tissue penetration, nutrient uptake and immune modulation in human sparganosis. In the present study, the expression of this enzyme was examined at different developmental stages of S. erinacei including immature egg, coracidium, plerocercoid in tadpole and rat, and adult. Proteolytic activity against carboxybenzoyl-phenylalanyl-arginyl-7-amino-4-methylcoumarin was detected in the extracts of coracidia and plerocercoid while no activity was observed in those of immature egg and adult. The specific activity in coracidial extracts was lower than that in the plerocercoid. Reverse transcription-polymerase chain reaction and Northern blot analysis demonstrated that the gene was expressed in the coracidium and plerocercoid but not in immature egg and adult. These results suggest that the 27 kDa cysteine protease is only expressed in the stages involving active migration of the parasite in the host tissue.

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  • Surgical treatment of a patient with live intracranial sparganosis for 17 years
    Jialing Hu, Kaili Liao, Xiaojin Feng, Danling Jiang, Hailin Liu, Qingcui Zheng, Hai Qiu, Fuzhou Hua, Guohai Xu, Chunhua Xu
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    Li Na Liu, Zhong Quan Wang, Xi Zhang, Peng Jiang, Xin Qi, Ruo Dan Liu, Zi Fang Zhang, Jing Cui, Xiao-Nong Zhou
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    Lei Deng, Pengju Xiong, Suokai Qian
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    Min Kyoung Cho, Keun Hee Lee, Sun Joo Lee, Se Won Kang, Mee Sun Ock, Yeon Chul Hong, Yong Seok Lee, Hak Sun Yu
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  • Comparison of carbohydrate moieties of sparganum proteins of the snake, mouse and those of adult worm
    Hyun Jong Yang
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The 10 kDa protein of Taenia solium metacestodes shows genus specific antigenicity
Seung-Kyu Park, Doo-Hee Yun, Joon-Yong Chung, Yoon Kong, Seung-Yull Cho
Korean J Parasitol 2000;38(3):191-194.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.191

Genus specific antigenicity of the 10 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was demonstrated by comparative immunoblot analysis. When CFs from taeniid metacestodes of T. saginata, T. solium, T. taeniaeformis and T. crassiceps were probed with specific monoclonal antibody (mAb) raised against 150 kDa protein of T. solium metacestodes, specific antibody reactions were observed in 7 and 10 kDa proteins of T. solium and in 7/8 kDa of T. saginata, T. taeniaeformis and T. crassiceps. The mAb did not react with any protein in hydatid fluid of Echinococcus granulosus and E. multilocularis. This result revealed that the 10 kDa peptide of T. solium metacestodes and its equivalent proteins of different Taenia metacestodes are genus specific antigens that are shared among different Taenia species.

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  • Echinococcus granulosus antigen B: A Hydrophobic Ligand Binding Protein at the host–parasite interface
    Valeria Silva-Álvarez, Ana Maite Folle, Ana Lía Ramos, Fernando Zamarreño, Marcelo D. Costabel, Eduardo García-Zepeda, Gustavo Salinas, Betina Córsico, Ana María Ferreira
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    Edda Sciutto, Gladis Fragoso, Marisela Hernández, Gabriela Rosas, José J. Martínez, Agnès Fleury, Jacquelynne Cervantes, Aline Aluja, Carlos Larralde
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Original Articles
IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis
Young Bae Chung, Yoon Kong, Hyun Jong Yang, Seung-Yull Cho
Korean J Parasitol 2000;38(3):145-150.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.145

Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially from experimental mice, fed 5 spargana each, were reacted with the crude extracts. Protein bands at 36-26 kDa and 103 kDa showed positive reactions since two weeks after infection. In a differential immunoblot, in which a monospecific antibody against sparganum chymase at 36 kDa was pre-treated, the reactions at 36-26 kDa disappeared, indicating that the sparganum chymase and its degradation products invoked IgG antibody reactions. When 69 patients sera of human sparganosis were examined for their IgG subclass responses, IgG4 levels showed the highest reaction which was followed by IgG1. The IgG4 antibody also reacted mainly with 36-31 kDa protease. These results indicate that 36 kDa chymase of S. mansoni plerocercoid is the main antigenic component inducing IgG antibody response in early stage of experimental sparganosis and for specific IgG subclass reactions in human sparganosis.

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    Vector-Borne and Zoonotic Diseases.2024;[Epub]     CrossRef
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    Oriol Jornet‐Rius, Nida Chornarm, Niki Skeldon, Ashley McGrew, Michael Lappin, Laia Solano‐Gallego, A Russell Moore
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    Yun Ku Kim, Myung Hwan Kim, Han Seoung Park, Ji Woong Jang, Yil Sik Hyun, Ji Hun Kim
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Persisting antibody reaction in paragonimiasis after praziquantel treatment is elicited mainly by egg antigens
Seung-Yull Cho, Yoon Kong, Doo-Hee Yun, Shin-Yong Kang, Lee-Soo Kim, Young-Bae Chung, Hyun-Jong Yang
Korean J Parasitol 2000;38(2):75-84.
Published online June 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.2.75

Antibody responses in serum and cerebrospinal fluid (CSF) samples from patients with active and chronic paragonimiasis and in sera from patients on whom follow-up studies were done after praziquantel treatment were analyzed using antigens of Paragonimus westermani prepared from eggs, metacercariae, juveniles of 4- and 7-week old, adult worms and recombinant protein of 28 kDa cruzipain-like cysteine protease (rPw28CCP). The patient sera/CSFs of active and chronic paragonimiasis revealed strong antibody reactions against the crude extracts of 4- and 7-week old juveniles as well as against those from egg and adult. rPw28CCP also showed specific reaction to the sera with active paragonimiasis. After the treatment, levels of specific antibodies in the sera gradually decreased to negative range in most patients. In some cases with persisting high antibody levels, however, the reactions at 27 kDa egg protein were sustained throughout the observation period of 34 months. The reactions at 35 and 32 kDa in adult extract and rPw28CCP disappeared rapidly after the treatment. Persistent antibody reactions even after successful treatment are provoked by continuous antigenic challenge from eggs which were not resolved by treatment.

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