Parasites, Hosts and Diseases

"monoclonal"

Original Articles

Development and Clinical Evaluation of a Rapid Diagnostic Test for Yellow Fever Non-Structural Protein 1: Yeong Hoon Kim, Tae-Yun Kim, Ji-Seon Park, Jin Suk Park, Jihoo Lee, Joungdae Moon, Chom-Kyu Chong, Ivan Neves Junior, Fernando Raphael Ferry, Hye-Jin Ahn, Lokraj Bhatt, Ho-Woo Nam; Korean J Parasitol 2019;57(3):283-290.
Published online June 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.3.283

Abstract
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A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclonal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in endemic areas and on the globe.

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Synthesis of Truncated DNA Aptamer and Its Application to an Electrochemical Biosensor Consisting of an Aptamer and a MXene Heterolayer for Yellow Fever Virus
Nayeon Kwon, Siyun Lee, Moonbong Jang, Jin-Ho Lee, Chulhwan Park, Taek Lee
BioChip Journal.2024; 18(1): 93. CrossRef
Challenges in Direct Detection of Flaviviruses: A Review
Bruna de Paula Dias, Camila Cavadas Barbosa, Cyntia Silva Ferreira, Samara Mayra Soares Alves dos Santos, Orlando Alfredo Pineda Arrieta, Wellington Carvalho Malta, Maria Laura Maximiano Dias Gomes, Mariela Alves e Silva, Júlia de Matos Fonseca, Lysandro
Pathogens.2023; 12(5): 643. CrossRef
A Chikungunya Virus Multiepitope Recombinant Protein Expressed from the Binary System Insect Cell/Recombinant Baculovirus Is Useful for Laboratorial Diagnosis of Chikungunya
Leonardo Assis da Silva, Monique da Rocha Queiroz Lima, Brenda Rabello de Camargo, Dyeferson Kened da Silva Coelho Guimarães, Anabele Azevedo Lima Barbastefano, Raquel Curtinhas de Lima, Paulo Vieira Damasco, Rivaldo Venâncio da Cunha, Luiz José de Souza,
Microorganisms.2022; 10(7): 1451. CrossRef

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Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus: Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Chom-Kyu Chong, Yanaihara Pinchemel, Francis Reisdo?rfer, Joyce Brito Coelho, Ronaldo Ferreira Dias, Pan Kee Bae, Zuinara Pereira Maia Gusma?o, Hye-Jin Ahn, Ho-Woo Nam; Korean J Parasitol 2018;56(1):61-70.
Published online February 28, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.1.61

Abstract
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We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV. These proteins were produced using baculovirus expression vector with Sf9 cells. Monoclonal antibodies J2G7 to NS1 and J5E1 to E protein were selected and conjugated with colloidal gold to produce the Zika IgG/IgM RDT kit (Zika RDT). Comparisons with ELISA, plaque reduction neutralization test (PRNT), and PCR were done to investigate the analytical sensitivity of Zika RDT, which resulted in 100% identical results. Sensitivity and specificity of Zika RDT in a field test was determined using positive and negative samples from Brazil and Korea. The diagnostic accuracy of Zika RDT was fairly high; sensitivity and specificity for IgG was 99.0 and 99.3%, respectively, while for IgM it was 96.7 and 98.7%, respectively. Cross reaction with dengue virus was evaluated using anti-Dengue Mixed Titer Performance Panel (PVD201), in which the Zika RDT showed cross-reactions with DENV in 16.7% and 5.6% in IgG and IgM, respectively. Cross reactions were not observed with West Nile, yellow fever, and hepatitis C virus infected sera. Zika RDT kit is very simple to use, rapid to assay, and very sensitive, and highly specific. Therefore, it would serve as a choice of method for point-of-care diagnosis and large scale surveys of ZIKV infection under clinical or field conditions worldwide in endemic areas.

Citations

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Development of a colloidal gold immunochromatographic strip to detect equine infectious anemia virus
Jianzhong Wang, Jicheng Qiu, Mengmeng Wang, Xiaojie Wu, Xiaoguang Li, Heng Zhang
Virology Journal.2025;[Epub] CrossRef
The Expanding Toolkit of Insect Cell Culture: A New Era in Biotechnology
Surjeet Kumar Arya, Cynthia L. Goodman, Subba Reddy Palli
Current Opinion in Insect Science.2025; : 101465. CrossRef
Advances in Metallic-Based Localized Surface Plasmon Sensors for Enhanced Tropical Disease Detection: A Comprehensive Review
Sajid Farooq, Denise Maria Zezell
Plasmonics.2024; 19(4): 1721. CrossRef
Diagnostic accuracy of DPP Fever Panel II Asia tests for tropical fever diagnosis
Sandhya Dhawan, Sabine Dittrich, Sonia Arafah, Stefano Ongarello, Aurelian Mace, Siribun Panapruksachat, Latsaniphone Boutthasavong, Aphaphone Adsamouth, Soulignasak Thongpaseuth, Viengmon Davong, Manivanh Vongsouvath, Elizabeth A. Ashley, Matthew T. Robi
PLOS Neglected Tropical Diseases.2024; 18(4): e0012077. CrossRef
Hyperendemic Dengue and Possible Zika Circulation in the Westernmost Region of the Indonesian Archipelago
Harapan Harapan, Kritu Panta, Alice Michie, Timo Ernst, Suzi McCarthy, Muhsin Muhsin, Safarianti Safarianti, Tjut Mariam Zanaria, Mudatsir Mudatsir, R. Tedjo Sasmono, Allison Imrie
Viruses.2022; 14(2): 219. CrossRef
Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology
Thai Leong Yap, Shin Yee Hong, Jun Hui Soh, Lekha Ravichandraprabhu, Vanessa W.X. Lim, Hsi-Min Chan, Tommy Z.X. Ong, Ying Ping Chua, Shi En Koh, Huajing Wang, Yee Sin Leo, Jackie Y. Ying, William Sun
Emerging Infectious Diseases.2021; 27(5): 1427. CrossRef
Development and characterization of mouse monoclonal antibodies targeting to distinct epitopes of Zika virus envelope protein for specific detection of Zika virus
Chia-Jung Li, Ping-Han Huang, Hui-Wen Chen, Shih-Chung Chang
Applied Microbiology and Biotechnology.2021; 105(11): 4663. CrossRef
Recent advances in point-of-care biosensors for the diagnosis of neglected tropical diseases
Patricia Batista Deroco, Dagwin Wachholz Junior, Lauro Tatsuo Kubota
Sensors and Actuators B: Chemical.2021; 349: 130821. CrossRef
Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies
Alexandra Marisa Targovnik, Jorge Alejandro Simonin, Gregorio Juan Mc Callum, Ignacio Smith, Franco Uriel Cuccovia Warlet, María Victoria Nugnes, María Victoria Miranda, Mariano Nicolás Belaich
Applied Microbiology and Biotechnology.2021; 105(21-22): 8195. CrossRef
Strategies for developing sensitive and specific nanoparticle-based lateral flow assays as point-of-care diagnostic device
Jun Hui Soh, Hsi-Min Chan, Jackie Y. Ying
Nano Today.2020; 30: 100831. CrossRef
Evolutions and upcoming on Zika virus diagnosis through an outbreak: A systematic review
Fernando A. Jorge, Mateus V. Thomazella, Deborah de Castro Moreira, Luciana D. G. Lopes, Jorge J. V. Teixeira, Dennis A. Bertolini
Reviews in Medical Virology.2020;[Epub] CrossRef
Zika virus serological diagnosis: commercial tests and monoclonal antibodies as tools
Isaura Beatriz Borges Silva, Aldacilene Souza da Silva, Mariana Sequetin Cunha, Aline Diniz Cabral, Kelly Cristina Alves de Oliveira, Elizabeth De Gaspari, Carlos Roberto Prudencio
Journal of Venomous Animals and Toxins including Tropical Diseases.2020;[Epub] CrossRef
ZIKV-Specific NS1 Epitopes as Serological Markers of Acute Zika Virus Infection
Yiu-Wing Kam, Juliana Almeida Leite, Siti Naqiah Amrun, Fok-Moon Lum, Wearn-Xin Yee, Farhana Abu Bakar, Kai Er Eng, David C Lye, Yee-Sin Leo, Chia-Yin Chong, Andre Ricardo Ribas Freitas, Guilherme Paier Milanez, Jose Luiz Proença-Modena, Laurent Rénia, Fa
The Journal of Infectious Diseases.2019; 220(2): 203. CrossRef
Seasonal dengue surge: Providers⬨tm) perceptions about the impact of dengue on patient volume, staffing and use of point of care testing in Indian emergency departments
Janice Blanchard, Katherine Douglass, Shweta Gidwani, Usha Khatri, Daniel Gaballa, Amelia Pousson, Neeraj Mangla, Jeffrey Smith
Journal of Infection and Public Health.2019; 12(6): 794. CrossRef
Development and Clinical Evaluation of a Rapid Diagnostic Test for Yellow Fever Non-Structural Protein 1
Yeong Hoon Kim, Tae-Yun Kim, Ji-Seon Park, Jin Suk Park, Jihoo Lee, Joungdae Moon, Chom-Kyu Chong, Ivan Neves Junior, Fernando Raphael Ferry, Hye-Jin Ahn, Lokraj Bhatt, Ho-Woo Nam
The Korean Journal of Parasitology.2019; 57(3): 283. CrossRef
Zika Fever: Development of Diagnostics, Prevention and Treatment
E. I. Kazachinskaya, D. V. Shan’shin, A. V. Ivanova
Problems of Particularly Dangerous Infections.2019; (2): 6. CrossRef
High correlation between Zika virus NS1 antibodies and neutralizing antibodies in selected serum samples from normal healthy Thais
Wannapa Sornjai, Suwipa Ramphan, Nitwara Wikan, Prasert Auewarakul, Duncan R. Smith
Scientific Reports.2019;[Epub] CrossRef
Generation and Characterization of a Polyclonal Antibody Against NS1 Protein for Detection of Zika Virus
Liding Zhang, Congjie Chen, Zhixin Chen, Shuzhen He, Yuzhu Song, Xueshan Xia, Qinqin Han, Jinyang Zhang
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact With trVLP Elements
Dong-Shan Yu, Tian-Hao Weng, Chen-Yu Hu, Zhi-Gang Wu, Yan-Hua Li, Lin-Fang Cheng, Nan-Ping Wu, Lan-Juan Li, Hang-Ping Yao
Frontiers in Microbiology.2018;[Epub] CrossRef
Analysis of Zika virus neutralizing antibodies in normal healthy Thais
Wannapa Sornjai, Janejira Jaratsittisin, Prasert Auewarakul, Nitwara Wikan, Duncan R. Smith
Scientific Reports.2018;[Epub] CrossRef

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Development of Monoclonal Antibodies for Diagnosis of Plasmodium vivax: Nguyen Thi Phuong Linh, Hyun Park, Jinyoung Lee, Dong-Xu Liu, Ga-Eun Seo, Hae-Jin Sohn, Jin-Hee Han, Eun-Taek Han, Ho-Joon Shin, Seon-Ju Yeo; Korean J Parasitol 2017;55(6):623-630.
Published online December 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.6.623

Abstract
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Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.

Citations

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Diagnostic Methods for Non-Falciparum Malaria
Alba Marina Gimenez, Rodolfo F. Marques, Matías Regiart, Daniel Youssef Bargieri
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Plasmodium falciparum Parasitemia and Band Sensitivity of the SD Bioline Malaria Ag P.f/Pan Rapid Diagnostic Test in Madagascar
Rajeev K. Mehlotra, Rosalind E. Howes, Estee Y. Cramer, Riley E. Tedrow, Tovonahary A. Rakotomanga, Stéphanie Ramboarina, Arsène C. Ratsimbasoa, Peter A. Zimmerman
The American Journal of Tropical Medicine and Hygiene.2019; 100(5): 1196. CrossRef

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Brief Communication

Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection: Mu-Xin Chen, Jia-Xu Chen, Shao-Hong Chen, Da-Na Huang, Lin Ai, Ren-Li Zhang; Korean J Parasitol 2016;54(3):375-380.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.375

Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.

Citations

Citations to this article as recorded by

Rapid Single-Step Immunochromatographic Assay for Angiostrongylus cantonensis Specific Antigen Detection
Praphathip Eamsobhana, Anchalee Tungtrongchitr, Darawan Wanachiwanawin, Sudarat Boonyong, Hoi-Sen Yong
Pathogens.2023; 12(6): 762. CrossRef
Semi-Automated Microfluidic Device Combined with a MiniPCR-Duplex Lateral Flow Dipstick for Screening and Visual Species Identification of Lymphatic Filariae
Achinya Phuakrod, Navapon Kusuwan, Witsaroot Sripumkhai, Pattaraluck Pattamang, Sirichit Wongkamchai
Micromachines.2022; 13(2): 336. CrossRef
Further studies of neuroangiostrongyliasis (rat lungworm disease) in Australian dogs: 92 new cases (2010–2020) and results for a novel, highly sensitive qPCR assay
Rogan Lee, Tsung-Yu Pai, Richard Churcher, Sarah Davies, Jody Braddock, Michael Linton, Jane Yu, Erin Bell, Justin Wimpole, Anna Dengate, David Collins, Narelle Brown, George Reppas, Susan Jaensch, Matthew K. Wun, Patricia Martin, William Sears, Jan Šlape
Parasitology.2021; 148(2): 178. CrossRef
Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis
Praphathip Eamsobhana, Anchalee Tungtrongchitr, Hoi-Sen Yong, Anchana Prasartvit, Darawan Wanachiwanawin, Xiao-Xian Gan
Parasitology.2021; 148(2): 234. CrossRef
Development of a recombinase polymerase amplification (RPA-EXO) and lateral flow assay (RPA-LFA) based on the ITS1 gene for the detection of Angiostrongylus cantonensis in gastropod intermediate hosts
Susan I. Jarvi, Elizabeth S. Atkinson, Lisa M. Kaluna, Kirsten A. Snook, Argon Steel
Parasitology.2021; 148(2): 251. CrossRef
Genetic Characterization and Detection of Angiostrongylus cantonensis by Molecular Approaches
Muxin Chen, Dana Huang, Jiaxu Chen, Yalan Huang, Huiwen Zheng, Yijun Tang, Qian Zhang, Shaohong Chen, Lin Ai, Xiaonong Zhou, Renli Zhang
Vector-Borne and Zoonotic Diseases.2021; 21(9): 643. CrossRef
Meningitis patients with Angiostrongylus cantonensis may present without eosinophilia in the cerebrospinal fluid in northern Vietnam
Tomoko Hiraoka, Ngo Chi Cuong, Sugihiro Hamaguchi, Mihoko Kikuchi, Shungo Katoh, Le Kim Anh, Nguyen Thi Hien Anh, Dang Duc Anh, Chris Smith, Haruhiko Maruyama, Lay-Myint Yoshida, Do Duy Cuong, Pham Thanh Thuy, Koya Ariyoshi, Alessandra Morassutti
PLOS Neglected Tropical Diseases.2020; 14(12): e0008937. CrossRef
Rapid diagnosis of parasitic diseases: current scenario and future needs
S. Momčilović, C. Cantacessi, V. Arsić-Arsenijević, D. Otranto, S. Tasić-Otašević
Clinical Microbiology and Infection.2019; 25(3): 290. CrossRef
Diagnostic approach to encephalitis and meningoencephalitis in adult returning travellers
A. Kenfak, G. Eperon, M. Schibler, F. Lamoth, M.I. Vargas, J.P. Stahl
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Survey of Angiostrongylus cantonensis Infection Status in Host Animals and Populations in Shenzhen, 2016–2017
Dana Huang, Yalan Huang, Yijun Tang, Qian Zhang, Xiaoheng Li, Shitong Gao, Wuwei Hua, Renli Zhang
Vector-Borne and Zoonotic Diseases.2019; 19(10): 717. CrossRef
Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis
Praphathip Eamsobhana, Anchalee Tungtrongchitr, Darawan Wanachiwanawin, Hoi-Sen Yong
International Journal of Infectious Diseases.2018; 73: 69. CrossRef
Small-scale spatial analysis of intermediate and definitive hosts of Angiostrongylus cantonensis
Qiu-An Hu, Yi Zhang, Yun-Hai Guo, Shan Lv, Shang Xia, He-Xiang Liu, Yuan Fang, Qin Liu, Dan Zhu, Qi-Ming Zhang, Chun-Li Yang, Guang-Yi Lin
Infectious Diseases of Poverty.2018;[Epub] CrossRef
Current Trends in Ligand Binding Real-Time Measurement Technologies
Stephanie Fraser, Judy Y. Shih, Mark Ware, Edward O’Connor, Mark J. Cameron, Martin Schwickart, Xuemei Zhao, Karin Regnstrom
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Original Articles

Monoclonal Antibody-Based Dipstick Assay: A Reliable Field Applicable Technique for Diagnosis of Schistosoma mansoni Infection Using Human Serum and Urine Samples: Zeinab Demerdash, Salwa Mohamed, Mohamed Hendawy, Ibrahim Rabia, Mohy Attia, Zeinab Shaker, Tarek M Diab; Korean J Parasitol 2013;51(1):93-98.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.93

Abstract
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A field applicable diagnostic technique, the dipstick assay, was evaluated for its sensitivity and specificity in diagnosing human Schistosoma mansoni infection. A monoclonal antibody (mAb) against S. mansoni adult worm tegumental antigen (AWTA) was employed in dipstick and sandwich ELISA for detection of circulating schistosome antigen (CSA) in both serum and urine samples. Based on clinical and parasitological examinations, 60 S. mansoni-infected patients, 30 patients infected with parasites other than schistosomiasis, and 30 uninfected healthy individuals were selected. The sensitivity and specificity of dipstick assay in urine samples were 86.7% and 90.0%, respectively, compared to 90.0% sensitivity and 91.7% specificity of sandwich ELISA. In serum samples, the sensitivity and specificity were 88.3% and 91.7% for dipstick assay vs. 91.7% and 95.0% for sandwich ELISA, respectively. The diagnostic efficacy of dipstick assay in urine and serum samples was 88.3% and 90.0%, while it was 90.8% and 93.3% for sandwich ELISA, respectively. The diagnostic indices of dipstick assay and ELISA either in serum or in urine were statistically comparable (P>0.05). In conclusion, the dipstick assay offers an alternative simple, rapid, non-invasive technique in detecting CSA or complement to stool examinations especially in field studies.

Citations

Citations to this article as recorded by

Construction of Camelus dromedaries Immune Single Domain Antibodies Library for Development of Schistosoma mansoni Specific Nanobodies Using Phage Display Strategy
Hadeer Adel El-Kalamawy, Mohammed H. Awwad, Tarek M. Diab, Hend Okasha, Amal M. Abdel-Kareim, Marawan A. Marawan, Salma A. Shoulah, Ehab El-Dabaa
Recent Patents on Biotechnology.2025; 19(1): 69. CrossRef
Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases
Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Serum low replacement medium versus serum rich replacement medium for production of Anti-Schistosoma Monoclonal antibody: A comparative study
Faten S. Mahmoud, Zeinab A. Demerdash, Sherihan M. Elmotawakel, Salwa Hassan, Mohamed Hendawy, Shimaa A. Atta, Shereen M. Shawky, Engy M. Alkhateeb, Hanaa I. Hassanin
Clinical Epidemiology and Global Health.2020; 8(2): 423. CrossRef
A protease-based biosensor for the detection of schistosome cercariae
A. J. Webb, R. Kelwick, M. J. Doenhoff, N. Kylilis, J. T. MacDonald, K. Y. Wen, C. McKeown, G. Baldwin, T. Ellis, K. Jensen, P. S. Freemont
Scientific Reports.2016;[Epub] CrossRef
Diagnostic Tests to Support Late-Stage Control Programs for Schistosomiasis and Soil-Transmitted Helminthiases
Kenneth R. Hawkins, Jason L. Cantera, Helen L. Storey, Brandon T. Leader, Tala de los Santos, Justin V. Remais
PLOS Neglected Tropical Diseases.2016; 10(12): e0004985. CrossRef
Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil
Maria Cristina Carvalho Espírito-Santo, Mónica Viviana Alvarado-Mora, Pedro Luiz Silva Pinto, Maria Carmen Arroyo Sanchez, Emmanuel Dias-Neto, Vera Lúcia Pagliusi Castilho, Elenice Messias do Nascimento Gonçalves, Pedro Paulo Chieffi, Expedito José de Alb
BioMed Research International.2015; 2015: 1. CrossRef
Diagnosis of Parasitic Infections: What’s Going On?
Alessandra Ricciardi, Momar Ndao
SLAS Discovery.2015; 20(1): 6. CrossRef

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Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients: Hye-Jin Ahn, Sera Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(2):89-96.
Published online June 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.2.89

Abstract
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Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy. The protein was not released into the parasitophorous vacuole during or after invasion. The cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg621. The full length cDNA sequence was completed with 5'-RACE as 1,592 bp, which contained open reading frame of 942 bp. The deduced amino acid sequence of Tg621 consisted of a polypeptide of 313 amino acids, with significant homology to ribosomal P proteins (RPP) of other organisms especially high to those of apicomplexan species. The expressed and purified TgRPP was assayed in western blot with the sera of toxoplasmosis patients and normal sera, which resulted in the 74.0% of positive reactions in toxoplasmosis patients whereas 8.3% in normal group. Therefore, the antibody formation against TgRPP in toxoplasmosis patients was regarded as specific for T. gondii infection and suggested a potential autoantibody.

Citations

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Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli
M. Serdal Sevinc, Veena Kumar, Makonnen Abebe, Susantha Mohottalage, Premkumari Kumarathasan, Renaud Vincent, Hari M. Vijay
Protein Expression and Purification.2009; 65(1): 8. CrossRef
Identification of ribosomal phosphoprotein P0 of Neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis
Houshuang Zhang, Eung-goo Lee, Min Liao, Muller K.A. Compaore, Guohong Zhang, Osamu Kawase, Kozo Fujisaki, Chihiro Sugimoto, Yoshifumi Nishikawa, Xuenan Xuan
Molecular and Biochemical Parasitology.2007; 153(2): 141. CrossRef
Enzyme-catalyzed amplified immunoassay for the detection of Toxoplasma gondii-specific IgG using Faradaic impedance spectroscopy, CV and QCM
Yanjun Ding, Hua Wang, Guoli Shen, Ruqin Yu
Analytical and Bioanalytical Chemistry.2005; 382(7): 1491. CrossRef
Host cell binding of GRA10, a novel, constitutively secreted dense granular protein from Toxoplasma gondii
Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam
Biochemical and Biophysical Research Communications.2005; 331(2): 614. CrossRef
The P Domain of the P0 Protein ofPlasmodium falciparumProtects against Challenge with Malaria Parasites
K. Rajeshwari, Kalpesh Patel, Savithri Nambeesan, Monika Mehta, Alfica Sehgal, Tirtha Chakraborty, Shobhona Sharma
Infection and Immunity.2004; 72(9): 5515. CrossRef

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Effects of specific monoclonal antibodies to dense granular proteins on the invasion of Toxoplasma gondii in vitro and in vivo: Dong Yeob Cha, In Kwan Song, Gye Sung Lee, Ok-Sun Hwang, Hyung-Jun Noh, Seung-Dong Yeo, Dae-Whan Shin, Young-Ha Lee; Korean J Parasitol 2001;39(3):233-240.
Published online September 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.3.233

Abstract
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Although some reports have been published on the protective effect of antibodies to Toxoplasma gondii surface membrane proteins, few address the inhibitory activity of antibodies to dense granular proteins (GRA proteins). Therefore, we performed a series of experiments to evaluate the inhibitory effects of monoclonal antibodies (mAbs) to GRA proteins (GRA2, 28 kDa; GRA6, 32 kDa) and surface membrane protein (SAG1, 30 kDa) on the invasion of T. gondii tachyzoites. Passive immunization of mice with one of three mAbs following challenge with a lethal dose of tachyzoites significantly increased survival compared with results for mice treated with control ascites. The survival times of mice challenged with tachyzoites pretreated with anti-GRA6 or anti-SAG1 mAb were significantly increased. Mice that received tachyzoites pretreated with both mAb and complement had longer survival times than those that received tachyzoites pretreated with mAb alone. Invasion of tachyzoites into fibroblasts and macrophages was significantly inhibited in the anti-GRA2, anti-GRA6 or anti-SAG1 mAb pretreated group. Pretreatment with mAb and complement inhibited invasion of tachyzoites in both fibroblasts and macrophages. These results suggest that specific antibodies to dense-granule molecules may be useful for controlling infection with T. gondii.

Citations

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Different kinetics of humoral response against individual antigens in the ovine model of Toxoplasma gondii infection and its pathological association
Paula Formigo, Ignacio Gual, Germán J. Cantón, Luisa F. Mendoza Morales, Emiliano Sosa, Agustina Soto Cabrera, Valeria Scioli, Claudia Morsella, Valeria A. Sander, Dadin P. Moore, Sergio O. Angel, Lucía M. Campero, Marina Clemente
Frontiers in Immunology.2025;[Epub] CrossRef
Immune system roles in pathogenesis, prognosis, control, and treatment of Toxoplasma gondii infection
Mohammad Mahdi Jafari, Zahra Azimzadeh Tabrizi, Mohammad Saaid Dayer, Nazanin Atieh Kazemi-Sefat, Mahshid Mohtashamifard, Rahimeh Mohseni, Atefeh Bagheri, Saeed Bahadory, Amir Karimipour-Saryazdi, Fatemeh Ghaffarifar
International Immunopharmacology.2023; 124: 110872. CrossRef
A Novel Vaccine Candidate: Recombinant Toxoplasma gondii Perforin-Like Protein 2 Stimulates Partial Protective Immunity Against Toxoplasmosis
Xiaowei Tian, Hanqi Sun, Meng Wang, Guangmin Wan, Tong Xie, Xuefang Mei, Zhenchao Zhang, Xiangrui Li, Shuai Wang
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Control of human toxoplasmosis
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Toxoplasma gondii: Ultrastructural localization of specific antigens and inhibition of intracellular multiplication by monoclonal antibodies: Boo-Young Lee, Myoung-Hee Ahn, Hyun-Chul Kim, Duk-Young Min; Korean J Parasitol 2001;39(1):67-75.
Published online March 31, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.1.67

Abstract
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This experiment was focused on the characterization of anti-Toxoplasma monoclonal antibodies (mAbs) and the effect of mAbs on the parasite invasion of mouse peritoneal macrophages. Twenty eight mAbs including M110, M556, R7A6 and M621 were characterized by Ab titer, immunoglobulin isotyping and western blot pattern. Antibody titer (optical density) of 4 mAbs, M110, M556, R7A6 and M621, were 0.53, 0.67, 0.45 and 0.39 (normal mouse serum; 0.19) with the same IgG1 isotypes shown by Enzyme-linked immunosorbent assay (ELISA). Western blot analysis showed that M110, M556, R7A6 and M621 reacted with the 33 kDa (p30), 31 kDa (p28), 43 kDa and 36 kDa protein. Immunogold labelling of mAbs M110, M556, R7A6 and M621 reacted with the surface membrane, dense granules and parasitophorous vacuolar membrane (PVM), rhoptries and cytoplasm of tachyzoite, respectively. For in vitro assay, preincubation of tachyzoites with four mAbs, M110, M556, R7A6 and M621 resulted in the decrease of the number of infected macrophages (p < 0.05) and the suppression of parasite multiplication at 18 h postinfection. Four monoclonal antibodies including M110 (SAG1) were found to have an important role in the inhibition of macrophage invasion and T. gondii multiplication in vitro, and these mAbs may be suitable for vaccine candidates, diagnostic kit and for chemotherapy.

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FERIAL ALAEDDINE, ANDREW HEMPHILL, KARIM DEBACHE, CHRISTOPHE GUIONAUD
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Yong-Feng Fu, Meng Feng, Kenji Ohnishi, Tamon Kimura, Johbu Itoh, Xun-Jia Cheng, Hiroshi Tachibana, J. H. Adams
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Effects of specific monoclonal antibodies to dense granular proteins on the invasion of Toxoplasma gondii in vitro and in vivo
Dong Yeob Cha, In Kwan Song, Gye Sung Lee, Ok-Sun Hwang, Hyung-Jun Noh, Seung-Dong Yeo, Dae-Whan Shin, Young-Ha Lee
The Korean Journal of Parasitology.2001; 39(3): 233. CrossRef

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Detection and characterization of excretory/secretory proteins from Toxoplasma gondii by monoclonal antibodies: Eui-Sun Son, Ho-Woo Nam; Korean J Parasitol 2001;39(1):49-56.
Published online March 31, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.1.49

Abstract
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Excretory/secretory proteins (ESP) from Toxoplasma gondii were analyzed to define the function in the penetration process into host cells. Whole ESP obtained at 37℃ were composed of 15 bands with molecular mass of 110, 97, 86, 80, 70, 60, 54, 42, 40, 36, 30, 28, 26, 22, and 19 kDa. Five ESP of 86, 80, 42, 36, and 28 kDa were reacted with monoclonal antibodies (mAb), named as Tg386 (microneme), Tg485 (surface membrane), Tg786 (rhoptry), Tg378, and Tg556 (both dense granules), respectively. The ESP was released by a temperature-dependent/-independent manner and all at once whenever ready to pour out except Tg786. Each ESP was not exhausted within the parasite but the amount was limited. Tg786 was released continuously with increment, whereas Tg378 and Tg556 were ceased to release after 3 and 4 hr. Dense granular Tg378 and Tg556 were released spontaneously and constitutively before the entry into host cells also. The entry of T. gondii was inhibited by all the mAbs differentially. And the parasite deprived of ESP was inhibited to enter exponentially up to 90.1%. It is suggested that ESP play an essential function to provide appropriate environment for the entry of the parasite into host cells.

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The Korean Journal of Parasitology.2001; 39(3): 233. CrossRef

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Brief Communication

The 10 kDa protein of Taenia solium metacestodes shows genus specific antigenicity: Seung-Kyu Park, Doo-Hee Yun, Joon-Yong Chung, Yoon Kong, Seung-Yull Cho; Korean J Parasitol 2000;38(3):191-194.
Published online September 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.3.191

Abstract
PDF

Genus specific antigenicity of the 10 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was demonstrated by comparative immunoblot analysis. When CFs from taeniid metacestodes of T. saginata, T. solium, T. taeniaeformis and T. crassiceps were probed with specific monoclonal antibody (mAb) raised against 150 kDa protein of T. solium metacestodes, specific antibody reactions were observed in 7 and 10 kDa proteins of T. solium and in 7/8 kDa of T. saginata, T. taeniaeformis and T. crassiceps. The mAb did not react with any protein in hydatid fluid of Echinococcus granulosus and E. multilocularis. This result revealed that the 10 kDa peptide of T. solium metacestodes and its equivalent proteins of different Taenia metacestodes are genus specific antigens that are shared among different Taenia species.

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Original Articles

Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA: Woon-Mok Sohn, Ho-Woo Nam; Korean J Parasitol 1999;37(4):249-256.
Published online December 31, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.4.249

Abstract
PDF

A total of 198 sera from stray cats was assayed against Toxoplasma gondii antigen by western blot. Out of 198 sera assayed, 26 sera (13.1%) showed typical blot patterns against T. gondii. When spotted by ELISA absorbance and indirect latex agglutination test (ILAT) titer, all 26 cases were distributed over the cut-off value of ELISA whereas 24 cases (92.3%) were in the positive range of 1:32 or higher and 2 cases in negative range by ILAT. Among western blot negative 172 sera, 162 cases were negative in both ILAT and ELISA while 10 cases were reactive falsely such that three cases were ILAT positive with 1:32 titer and 9 cases were ELISA positive (2 cases overlapped). These 10 cases reacted peculiarly without typical binding pattern in Western blot. Sandwich-ELISA was performed with monoclonal antibodies (mAbs) of Tg563 (30 kDa, SAG1), Tg505 (22 kDa, SAG2), Tg605 (43 kDa, SAG3), Tg556 (28 kDa, GRA2), Tg737 (32 kDa, GRA6), Tg695 (66 kDa, ROP2), Tg786 (42 kDa, ROP6), and Tg621 (32 kDa, anonymous but cytosolic) clone, respectively. All western blot-positive cases were in the positive range and negative cases in the negative range clearly. Among the 10 false reactive cases, 3 cases were in the positive range with one or more mAbs. All mAbs used in this study were confirmed to be specific to T. gondii infection as a standardized sandwich-ELISA to differentiate it from other pathogens.

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Monoclonal antibodies to recombinant Der p 2, a major house dust mite allergen: specificity, epitope analysis and development of two-site capture ELISA: Tai-Soon Yong, Sang-Mi Lee, Gab-Man Park, In-Yong Lee, Han-Il Ree, Kyung-Sup Kim, Sang-Hwan Oh, Jung-Won Park, Chein-Soo Hong; Korean J Parasitol 1999;37(3):163-169.
Published online September 20, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.3.163

Abstract
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House dust mite allergens have been well established as sensitizing agents that are important in the induction of allergic diseases. In order to analyze epitopes of the allergen and to develop a quantitative method of the allergen exposure, monoclonal antibodies against a recombinant Der p 2 (rDer p 2), one of the major allergens of Dermatophagoides pteronyssinus, were produced. Four monoclonal antibodies produced were species-specific and did not cross-react to the D. farinae crude extract. Two of the monoclonal antibodies were found to be IgG1 and the others were IgM. For the analysis of epitopes, a Der p 2 cDNA encoding 126 amino acids (aa) was dissected into three fragments with several overlapping peptides, A (aa residues 1-49), B (44-93), and C fragment (84-126). Three monoclonal antibodies showed reactivities to the recombinant B fragment and to the full-length rDer p 2, but one monoclonal antibody reacted only with the full-length rDer p 2. Two-site capture ELISA was developed using two different monoclonal antibodies for quantitating Der p 2 in house dust. The sensitivity limit was 4 ng/ml with rDer p 2 and 8 ?g/ml with the D. pteronyssinus crude extract. The result suggested that the assay using monoclonal antibodies against rDer p 2 could be useful for the environmental studies and for the standardization of mite allergen extracts.

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