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Original Article

Phylogenetic and phylogeographic analyses of Anisakis simplex sensu stricto (Nematoda: Anisakidae) from the common minke whale in Korean waters
Sunmin Kim, Bom Sok Lee, Seongjun Choe
Parasites Hosts Dis 2023;61(3):240-250.
Published online August 21, 2023
DOI: https://doi.org/10.3347/PHD.23046
The genus Anisakis is among the most significant parasites to public health, as it causes anisakiasis, a parasitic infection in humans resulting from consuming raw or undercooked seafood. Although the infection status of i>Anisakis in second intermediate hosts, such as marine fishes and cephalopods, and humans have been severally reported in Korea, no information about the definitive host in Korean waters is available. In 2014, 2 adult gastric nematodes were collected from a common minke whale (Balaenoptera acutorostrata) found in the East Sea, Korea. These worms were identified as A. simplex sensu stricto (s.s.) by comparing the mitochondrial COX2 marker with previously deposited sequences. Phylogenetic and phylogeographic analyses of A. simplex (s.s.) worldwide revealed 2 distinct populations: the Pacific population and the European waters population. This is the first report on adult i>Anisakis and its definitive host species in Korea. Further studies on Anisakis infection in other cetacean species and marine mammals in Korean seas are warrantedi>Anisakis

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  • Genetic analyses of Anisakis pegreffii (Nematoda: Anisakidae) from the East Asian finless porpoise Neophocaena asiaeorientalis sunameri (Cetacea: Phocoenidae) in Korean waters
    Sunmin Kim, Jong Yoon Jeon, Kyunglee Lee, Hyunjoo Lee, Han Chan Park, Kyung Eun Lee, Hang Lee, Sung Bin Lee, Sang Wha Kim, Se Chang Park, Seongjun Choe, Heejeong Youn
    Parasitology Research.2024;[Epub]     CrossRef
  • 6,432 View
  • 177 Download
  • 1 Web of Science
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Brief Communication

Molecular epidemiology of Toxoplasma gondii in cattle in Korea
Kyoo-Tae Kim, Min-Goo Seo
Parasites Hosts Dis 2023;61(2):210-215.
Published online May 23, 2023
DOI: https://doi.org/10.3347/PHD.23016
Toxoplasmosis is a major public health concern, with raw or undercooked meat being the primary source of human infection. Knowledge regarding the molecular epidemiology of Toxoplasma gondii in cattle destined for human consumption in Korea is lacking. The present study aimed to genetically characterize the infectious strains of T. gondii. Overall, 455 cattle blood samples from 84 farms in the Gyeongnam-do (Province) were randomly collected in 2017. Nested PCR analysis revealed that only 3 (0.7%) samples were infected with T. gondii. The B1 gene sequence of T. gondii was observed to be similar (97.3–99.6%) to that of other T. gondii isolates. This is the first study to perform the molecular detection of T. gondii in cattle in Korea. Although the prevalence of infection was low, our findings suggest that cattle present a potential public health issue. It may be crucial to recognize the importance of T. gondii infection in cattle meat.

Citations

Citations to this article as recorded by  Crossref logo
  • Molecular detection of Toxoplasma gondii in ticks and their respective host dogs
    Min-Goo Seo, Dongmi Kwak
    Parasites, Hosts and Diseases.2025; 63(1): 66.     CrossRef
  • Prevalence and molecular characterization of intestinal parasites in shelter dogs from South Korea
    You-Jeong Lee, Beoul Kim, Gyeonghyeon Lee, Yong-Myung Kang, Dongmi Kwak, Min-Goo Seo
    Research in Veterinary Science.2025; 188: 105620.     CrossRef
  • Antileishmanial and Antitoxoplasmal Activities of 1,4-Dihydropyridines
    Thaís A. S. Oliveira, Yan R. Robles, Ibrahim S. Al Nasr, Waleed S. Koko, Tariq A. Khan, Ismail Daoud, Seyfeddine Rahali, Noureddine Amdouni, Ridha B. Said, Antônio E. M. Crotti
    ACS Omega.2025; 10(28): 31066.     CrossRef
  • Tick-borne pathogens in Mongolian ticks: The high prevalence of Rickettsia raoultii and its public health implications
    You-Jeong Lee, Beoul Kim, Dongmi Kwak, Ochirkhuu Nyamsuren, Nyamdavaa Guugandaa, Min-Goo Seo
    Acta Tropica.2024; 260: 107412.     CrossRef
  • 4,152 View
  • 165 Download
  • 4 Web of Science
  • Crossref

Original Articles

Morphological Identification and Phylogenetic Analysis of Laelapin Mite Species (Acari: Mesostigmata: Laelapidae) from China
Huijuan Yang, Zhihua Yang, Wenge Dong
Korean J Parasitol 2022;60(4):273-279.
Published online August 24, 2022
DOI: https://doi.org/10.3347/kjp.2022.60.4.273
Laelapinae mites are involved in transmission of microbial diseases between wildlife and humans, with an impact on public health. In this study, 5 mite members in the subfamily Laelapinae (laelapin mites; LM) were morphologically identified by light microscopy, and the phylogenetic relationship of LM was analyzed in combination with the sequence information of part of the LM cytochrome oxidase subunit I (cox1) gene. The morphological identification revealed that 5 mites belonged to the genera Laelaps and Haemolaelaps, respectively. Sequence analysis showed that the ratio of non-synonymous mutation rate to synonymous mutation rate of LM was less than 1, indicating that the LM cox1 gene had undergone purifying selection. Phylogenetic analysis showed that the Laelapinae is a monophyletic group. The genera Haemolaelaps and Hyperlaelaps did not separated into distinct clades but clustered together with species of the genus Laelaps. Our morphological and molecular analyses to describe the phylogenetic relationships among different genera and species of Laelapinae provide a reference for the improvement and revision of the LM taxonomy system.

Citations

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  • First Record of Phoretic Mite from Red Palm Weevil Rhynchophorus ferrugiuneus (Olivier,1790) from Basrah, Southern Iraq
    Shurooq Abdullah Najim, Hussain Ali Jabbar, Basil Yousuf Mahdi
    Ibn AL-Haitham Journal For Pure and Applied Sciences.2024; 37(1): 94.     CrossRef
  • The first complete mitochondrial genome of the genus Laelaps with novel gene arrangement reveals extensive rearrangement and phylogenetics in the superfamily Dermanyssoidea
    Bili Yuan, Gangxian He, Wenge Dong
    Experimental and Applied Acarology.2024; 93(3): 515.     CrossRef
  • Comparative analysis of the mitochondrial genome of Dermacentor steini from different regions in China
    Huijuan Yang, Ting Chen, Wenge Dong
    Parasitology.2023; 150(2): 195.     CrossRef
  • Complete mitochondrial genome of Penicillidia jenynsii (Diptera: Hippoboscoidea: Nycteribiidae) and phylogenetic relationship
    Jinting Yang, Xiaobin Huang, Yujuan Wang, Huijuan Yang, Xianzheng Zhang, Xiaoyan Zheng
    Parasitology.2023; 150(7): 623.     CrossRef
  • The first complete mitochondrial genome of the genus Echinolaelaps reveals mitochondrial genome rearrangement type and evolution of Gamasida
    Bili Yuan, Gangxian He, Wenge Dong
    Parasitology.2023; 150(7): 644.     CrossRef
  • The complete mitochondrial genome of Eulaelaps huzhuensis (Mesostigmata: Haemogamasidae)
    Hui-Juan Yang, Zhi-Hua Yang, Tian-Guang Ren, Wen-Ge Dong
    Experimental and Applied Acarology.2023; 90(3-4): 301.     CrossRef
  • Molecular Identification and Phylogenetic Analysis of Laelapidae Mites (Acari: Mesostigmata)
    Evelina Kaminskienė, Jana Radzijevskaja, Loreta Griciuvienė, Michal Stanko, Justina Snegiriovaitė, Dalytė Mardosaitė-Busaitienė, Algimantas Paulauskas
    Animals.2023; 13(13): 2185.     CrossRef
  • 5,557 View
  • 205 Download
  • 7 Web of Science
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Epidemiological Characteristics of Rodents and Chiggers with Orientia Tsutsugamushi in the Republic of Korea
Jung Wook Park, Dae Sung Yu, Gi Seong Lee, Jin Jong Seo, Jae Keun Chung, Jae Il Lee
Korean J Parasitol 2020;58(5):559-564.
Published online October 22, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.5.559
A survey of rodents and chiggers associated with Orientia tsutsugamushi was conducted in a rural region of the Republic of Korea (Korea) between 2014 and 2018. Overall Apodemus agrarius 15.2% had the highest seropisitive for O. tsutsugamushi, followed by Myodes regulus 11.4%. Monthly risk factors using logistic regression analysis were not associated with O. tsutsugamushi infections in rodents. The overall prevalence rate of O. tsutsugamushi among chiggers was 0.3%. The chigger (Leptotrombidium scutellare) and monthly (October) risk factors were associated with O. tsutsugamushi human infections (P<0.05). Orientia tsutsugamushi infections are endemic in rodents in Korea and people, for example, soldiers who are active outdoors, must employ preventive measures, especially during October (P<0.05). When there are many reports of O. tsutsugamushi infections in Korea. The Boryong strain 85.7% (2/14) was the most common strain detected in chiggers, followed by the Shimokoshi 7.1% (1/14) and Karp 7.1% strains.

Citations

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  • Mite Infestation on Rattus tanezum rats in southwest China concerning risk models
    Ru-Jin Liu, Xian-Guo Guo, Pei-Ying Peng, Yan Lv, Peng-Wu Yin, Wen-Yu Song, Rong Xiang, Yan-Ling Chen, Bei Li, Dao-Chao Jin
    Frontiers in Veterinary Science.2025;[Epub]     CrossRef
  • Geographic distribution of Orientia tsutsugamushi strains on chigger mites in the Republic of Korea (2021–2023)
    Hyeon Seung Lee, Byung-Eon Noh, Hyunwoo Kim, Heeil Lee
    Parasites & Vectors.2025;[Epub]     CrossRef
  • A cross sectional study on molecular prevalence of Orientia tsutsugamushi in household rat population of South India
    S. Purushothaman, P. Azhahianambi, M. Dharman, R. Gokula Kannan, K.G. Tirumurugaan, C. Soundararajan, R.P. Aravindh Babu, Panneer Devaraju, John A.J. Prakash
    Comparative Immunology, Microbiology and Infectious Diseases.2024; 111: 102212.     CrossRef
  • Orientia tsutsugamushi Infection in Wild Small Mammals in Western Yunnan Province, China
    Yun-Yan Luo, Si-Tong Liu, Qi-Nan He, Ru-Dan Hong, Jun-Jie Zhu, Zhi-Qiong Ai, Jia-Xiang Yin
    Pathogens.2023; 12(1): 128.     CrossRef
  • Detection of Orientia tsutsugamushi in Novel Trombiculid Mite Species in Northern Tamil Nadu, India: Use of Targeting the Multicopy traD Gene
    John Antony Jude Prakash, Kannan Kamarasu, P Philip Samuel, Renu Govindarajan, Punitha Govindasamy, Liji Anna Johnson, P Ramalingam, J Nirmalson, K C Seran, Kevin Macaluso
    Journal of Medical Entomology.2022; 59(2): 693.     CrossRef
  • Buerger’s Disease May be a Chronic Rickettsial Infection with Superimposed Thrombosis: Literature Review and Efficacy of Doxycycline in Three Patients
    Moon-Hyun Chung, Jin-Soo Lee, Jae-Seung Kang
    Infection & Chemotherapy.2022; 54(1): 20.     CrossRef
  • Nationwide Incidence of Chigger Mite Populations and Molecular Detection of Orientia tsutsugamushi in the Republic of Korea, 2020
    Min-Goo Seo, Bong-Goo Song, Tae-Kyu Kim, Byung-Eon Noh, Hak Seon Lee, Wook-Gyo Lee, Hee Il Lee
    Microorganisms.2021; 9(8): 1563.     CrossRef
  • Geographical Distribution and Epidemiologic Factors of Chigger Mites on Apodemus agrarius during Autumn in Korea
    In Yong Lee, Jae-Won Lim, Jang Hoon Seo, Heung Chul Kim, Ku Jae Lee, Tai-Soon Yong, Won-Ja Lee, Jae-Ran Yu, Seobo Sim
    The Korean Journal of Parasitology.2021; 59(5): 473.     CrossRef
  • 5,318 View
  • 91 Download
  • 8 Web of Science
  • Crossref

Case Report

Identification of Cystoisospora ohioensis in a Diarrheal Dog in Korea
Sangmin Lee, Junki Kim, Doo-Sung Cheon, Eun-A Moon, Dong Joo Seo, Soontag Jung, Hansaem Shin, Changsun Choi
Korean J Parasitol 2018;56(4):371-374.
Published online August 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.4.371
A 3-month-old female Maltese puppy was hospitalized with persistent diarrhea in a local veterinary clinic. Blood chemistry and hematology profile were analyzed and fecal smear was examined. Diarrheal stools were examined in a diagnostic laboratory, using multiplex real-time polymerase chain reaction (PCR) against 23 diarrheal pathogens. Sequence analysis was performed using nested PCR amplicon of 18S ribosomal RNA. Coccidian oocysts were identified in the fecal smear. Although multiplex real-time PCR was positive for Cyclospora cayetanensis, the final diagnosis was Cystoisospora ohioensis infection, confirmed by phylogenetic analysis of 18S rRNA. To our knowledge, this the first case report of C. ohioensis in Korea, using microscopic examination and phylogenetic analysis.

Citations

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  • 18S rRNA gene metabarcoding for investigation of gastrointestinal parasite diversity in great cormorants
    Subin Lee, Badriah Alkathiri, Chang Hyeon Lee, Heon Woo Lee, Dong-Hyuk Jeong, Ju Yeong Kim, Seongjun Choe, Seung-Hun Lee
    Scientific Reports.2025;[Epub]     CrossRef
  • Morphological and molecular biology identification of Cystoisospora sp. in the blue fox, Alopex lagopus (Linnaeus, 1758)
    Yifan Zhang, Ziyang Qin, Kaihui Zhang, Jiashu Lang, Nanhao Wang, Yixuan Niu, Longxian Zhang
    Parasitology Research.2024;[Epub]     CrossRef
  • Coccidiosis in dogs—100 years of progress
    Jitender P. Dubey, David S. Lindsay
    Veterinary Parasitology.2019; 266: 34.     CrossRef
  • 9,549 View
  • 121 Download
  • 3 Web of Science
  • Crossref

Original Articles

First Molecular Characterization of Hypoderma actaeon in Cattle and Red Deer (Cervus elaphus) in Portugal
Haroon Ahmed, S?rgio Ramalho Sousa, Sami Simsek, Sofia Anast?cio, Seyma Gunyakti Kilinc
Korean J Parasitol 2017;55(6):653-658.
Published online December 31, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.6.653
Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The
objective
of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.

Citations

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  • Morphological and Molecular Identification of Obligatory Myiasis-Causing Species in Wild Cervids in Croatia
    Ema Gagović, Daria Jurković Žilić, Krunoslav Pintur, Adnan Hodžić, Šimun Naletilić, Relja Beck
    Animals.2025; 15(2): 208.     CrossRef
  • Assesment of Hypoderma infestation in a wild population of Cervus elaphus from mountains Atlantic ecosystems in southwestern Europe (Spain)
    Sara González, Rosario Panadero, María Luisa Del Rio, María Natividad Díez, María del Rosario Hidalgo, Angélica Martínez
    Veterinary Research Communications.2024; 48(2): 761.     CrossRef
  • Molecular characterization of the camel nasal botfly, Cephalopina titillator (Diptera: Oestridae)
    Mona G. Shaalan, Sherif Hamed Farghaly, Emad I. Khater, Mohamed A. Kenawy, Enas Hamdy Ghallab
    Beni-Suef University Journal of Basic and Applied Sciences.2024;[Epub]     CrossRef
  • Identification of Hypoderma actaeon (Diptera: Oestridae) in red deer (Cervus elaphus) from northern Spain: Microscopy study and molecular analysis
    Sara González, Maria Luisa Del Rio, Maria Natividad Diez, Maria del Rosario Hidalgo, Angelica Martínez
    Microscopy Research and Technique.2023; 86(1): 3.     CrossRef
  • Temporal and spatial spread of Hypoderma actaeon infection in roe deer from peninsular Spain determined by an indirect enzyme‐linked immunosorbent assay
    R. Panadero, C. M. López, S. Remesar, E. Cabanelas, G. Varas, F. Markina, P. Díaz, D. García‐Dios, A. Prieto, G. Fernández, P. Díez‐Baños, P. Morrondo
    Medical and Veterinary Entomology.2020; 34(1): 44.     CrossRef
  • 8,661 View
  • 155 Download
  • 8 Web of Science
  • Crossref
Genetic Diversity and Phylogenetic Analysis of the Iranian Leishmania Parasites Based on HSP70 Gene PCR-RFLP and Sequence Analysis
Sara Nemati, Asghar Fazaeli, Homa Hajjaran, Ali Khamesipour, Mohsen Falahati Anbaran, Arezoo Bozorgomid, Fatah Zarei
Korean J Parasitol 2017;55(4):367-374.
Published online August 31, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.4.367
Despite the broad distribution of leishmaniasis among Iranians and animals across the country, little is known about the genetic characteristics of the causative agents. Applying both HSP70 PCR-RFLP and sequence analyses, this study aimed to evaluate the genetic diversity and phylogenetic relationships among Leishmania spp. isolated from Iranian endemic foci and available reference strains. A total of 36 Leishmania isolates from almost all districts across the country were genetically analyzed for the HSP70 gene using both PCR-RFLP and sequence analysis. The original HSP70 gene sequences were aligned along with homologous Leishmania sequences retrieved from NCBI, and subjected to the phylogenetic analysis. Basic parameters of genetic diversity were also estimated. The HSP70 PCR-RFLP presented 3 different electrophoretic patterns, with no further intraspecific variation, corresponding to 3 Leishmania species available in the country, L. tropica, L. major, and L. infantum. Phylogenetic analyses presented 5 major clades, corresponding to 5 species complexes. Iranian lineages, including L. major, L. tropica, and L. infantum, were distributed among 3 complexes L. major, L. tropica, and L. donovani. However, within the L. major and L. donovani species complexes, the HSP70 phylogeny was not able to distinguish clearly between the L. major and L. turanica isolates, and between the L. infantum, L. donovani, and L. chagasi isolates, respectively. Our results indicated that both HSP70 PCR-RFLP and sequence analyses are medically applicable tools for identification of Leishmania species in Iranian patients. However, the reduced genetic diversity of the target gene makes it inevitable that its phylogeny only resolves the major groups, namely, the species complexes.

Citations

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  • Existing and newly emerging human cutaneous Leishmania isolates in Ethiopia: a systematic review
    Abiy Ayele Angelo, Getu Girmay, Dereje Mengesha Berta, Bisrat Birke Teketelew, Elias Chane, Negesse Cherie, Muluneh Assefa, Zufan Yiheyis Abriham, Mebratu Tamir
    Discover Medicine.2025;[Epub]     CrossRef
  • Update on the Seroepidemiology of Human Cystic Echinococcosis and Associated Risk Factors in Iran: A Systematic Review and Meta‐Analysis
    Nashmin Mohemmi, Mohammad Taghi Khodayari, Seyed Ahmad Karamati, Mohammad Reza Shiee, Hossein Effatpanah, Afshin Davari, Ali Reza Ghorbani, Arezoo Bozorgomid
    Health Science Reports.2025;[Epub]     CrossRef
  • Genetic Diversity and Chromosomal Variations in the Iranian Leishmania major Strain: Insights into Pathogenicity and Drug Resistance
    Hanieh Sharifian, Anis Khalafiyan, Mahmood Fadaie, Hossein Khanahmad, Zabihollah Shahmoradi, Erfan Zaker, Parisa Mousavi, Nadia Pourmoshir, Azadeh Zolfaghari
    Advanced Biomedical Research.2025;[Epub]     CrossRef
  • Genetic diversity and epidemiological insights into cutaneous leishmaniasis in Pakistan: a comprehensive study on clinical manifestations and molecular characterization of Leishmania species
    Shumaila Naz, Muhammed Nalcaci, Obaid Hayat, Seray Toz, Azhar Minhas, Shahid Waseem, Yusuf Ozbel
    Parasitology Research.2024;[Epub]     CrossRef
  • Assessment of genetic markers for multilocus sequence typing (MLST) of Fasciola isolates from Iran
    Naser Nazari, Mohamad Bagher Rokni, Madoka Ichikawa‐Seki, Saber Raeghi, Homa Hajjaran, Shahab Falahi, Yazdan Hamzavi, Peyman Heydarian, Afshin Davari, Keyphobad Ghadiri, Arezoo Bozorgomid
    Veterinary Medicine and Science.2023; 9(2): 924.     CrossRef
  • Real‐time impact of COVID‐19 pandemic on cutaneous leishmaniasis case finding and strategic planning, preventive interventions, control and epidemiology in a region with a high burden of cutaneous leishmaniasis and COVID‐19: A cross‐sectional descriptive
    Morteza Shams, Ayoub Rashidi, Jasem Mohamadi, Mohamad Moradi, Reza Pakzad, Razi Naserifar, Jahangir Abdi, Fariba Ghelichi, Arezoo Bozorgomid, Nahid Maspi, Azra Kenarkoohi, Yasin Mohammadi, Amir Abdoli, Shahab Falahi
    Health Science Reports.2023;[Epub]     CrossRef
  • An overview of the trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites infecting several mammal species in Colombia
    Adriana C. Castillo-Castañeda, Luz H. Patiño, Maria Fernanda Zuñiga, Omar Cantillo-Barraza, Martha S. Ayala, Maryi Segura, Jessica Bautista, Plutarco Urbano, Jeiczon Jaimes-Dueñez, Juan David Ramírez
    Parasites & Vectors.2022;[Epub]     CrossRef
  • Taxonomy, Population Structure and Genetic Diversity of Iranian Leishmania Strains of Cutaneous and Visceral Leishmaniasis
    Sara Nemati, Homa Hajjaran, Soudabeh Heydari, Asghar Fazaeli, Ali Khamesipour, Mohsen Falahati Anbaran, Mehdi Mohebali, Hamed Mirjalali
    Acta Parasitologica.2021; 66(4): 1274.     CrossRef
  • The Geographical Distribution of Human Cutaneous and Visceral Leishmania Species Identified by Molecular Methods in Iran: A Systematic Review With Meta-Analysis
    Homa Hajjaran, Reza Saberi, Alireza Borjian, Mahdi Fakhar, Seyed Abdollah Hosseini, Sajjad Ghodrati, Mehdi Mohebali
    Frontiers in Public Health.2021;[Epub]     CrossRef
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  • Isolation and Molecular Identification of Leishmania spp. in Patients With Cutaneous Leishmaniasis in Golestan Province, Iran
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    Veterinary World.2018; : 1692.     CrossRef
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  • 234 Download
  • 13 Web of Science
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Brief Communication

PCR Identification and Phylogenetic Analysis of Trichomonas gallinae from Domestic Pigeons in Guangzhou, China
Shen-Ben Qiu, Meng-Na Lv, Xi He, Ya-Biao Weng, Shang-Shu Zou, Xin-Qiu Wang, Rui-Qing Lin
Korean J Parasitol 2017;55(3):333-336.
Published online June 30, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.3.333
Avian trichomoniasis caused by Trichomonas gallinae is a serious protozoan disease worldwide. The domestic pigeon (Columba livia domestica) is the main host for T. gallinae and plays an important role in the spread of the disease. Based on the internal transcribed spacers of nuclear ribosomal DNA of this parasite, a pair of primers (TgF2/TgR2) was designed and used to develop a PCR assay for the diagnosis of T. gallinae infection in domestic pigeons. This approach allowed the identification of T. gallinae, and no amplicons were produced when using DNA from other common avian pathogens. The minimum amount of DNA detectable by the specific PCR assay developed in this study was 15 pg. Clinical samples from Guangzhou, China, were examined using this PCR assay and a standard microscopy method, and their molecular characteristics were determined by phylogenetic analysis. All of the T. gallinae-positive samples detected by microscopic examination were also detected as positive by the PCR assay. Most of the samples identified as negative by microscopic examination were detected as T. gallinae positive by the PCR assay and were confirmed by sequencing. The positive samples of T. gallinae collected from Guangzhou, China, were identified as T. gallinae genotype B by sequencing and phylogenetic analyses, providing relevant data for studying the ecology and population genetic structures of trichomonads and for the prevention and control of the diseases they cause.

Citations

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  • Rapid and sensitive detection of Trichomonas gallinae using RAA-CRISPR-Cas12a
    Yuhan Zhou, Yaqian Chen, Xinglong Song, Zhenyu Zhong, Qingyun Guo, Shengfan Jing, Olalekan Opeyemi Ayanniyi, Zhenxiao Lu, Qingxun Zhang, Congshan Yang
    Veterinary Parasitology.2025; 334: 110412.     CrossRef
  • Recombinase-Aided Amplification Combined with Lateral Flow Dipstick for the Rapid and Sensitive Detection of Trichomonas gallinae
    Yuhan Zhou, Xinglong Song, Yaqian Chen, Zhenyu Zhong, Qingyun Guo, Shengfan Jing, Yilei Zhang, Qingxun Zhang, Congshan Yang
    Journal of Parasitology.2025;[Epub]     CrossRef
  • An Evolutionarily Conserved Laterally Acquired Toolkit Enables Microbiota Targeting by Trichomonas
    Adam J Hart, Lenshina A Mpeyako, Nick P Bailey, George Merces, Joseph Gray, Jacob Biboy, Manuel Banzhaf, Waldemar Vollmer, Robert P Hirt, Ana Carolina Junqueira
    Molecular Biology and Evolution.2025;[Epub]     CrossRef
  • Genotypic diversity and epidemiology of Trichomonas gallinae in Columbidae: Insights from a comprehensive analysis
    Yu Liu, Haiming Cai, Dingai Wang, Shenquan Liao, Nanshan Qi, Juan Li, Zhuanqiang Yan, Hanqin Shen, Siyun Fang, Minna Lv, Xuhui Lin, Yongle Song, Junjing Hu, Yibin Zhu, Xiangjie Chen, Lijun Yin, Jianfei Zhang, Yaqiong Guo, Mingfei Sun
    International Journal for Parasitology: Parasites and Wildlife.2024; 23: 100918.     CrossRef
  • Prevalence and molecular characterization of Trichomonas gallinae from pigeons in Anhui, China
    Yilei Zhang, Zhenxiao Lu, Zhenzhen Liu, Yuhan Zhou, Guodong Xiao, Ayanniyi Olalekan Opeyemi, Sihua Jin, Yongsheng Li, Tao Liu, Qiong Wu, Xingdong Sun, Qianming Xu, Qingxun Zhang, Congshan Yang
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  • SURVEY ON THE MOST COMMON DISEASES CIRCULATING AMONG PIGEONS IN THE EASTERN PROVINCE, SAUDI ARABIA
    Mahmoud H.A.Mohamad, Adel M.Abdel Aziz
    The Journal of Animal and Plant Sciences.2023; (5): 1220.     CrossRef
  • Genetic characterization of Trichomonas gallinae (Rivolta, 1878) in companion birds in Japan and the genotypical relationship in the Asia region
    Shyun Chou, Shinichiro Hadano, Atsushi Kojima, Mario Yorisaki, Masaru Yasuda, Kazunori Ike, Toshihiro Tokiwa
    Journal of Microbiology, Immunology and Infection.2022; 55(3): 527.     CrossRef
  • A new duplex real-time PCR for simultaneous detection and differentiation of Tetratrichomonas gallinarum and Trichomonas gallinae
    Brigitte Sigrist, T. W. Cherry Ng, Sarah Albini, Nina Wolfrum
    Journal of Veterinary Diagnostic Investigation.2022; 34(4): 631.     CrossRef
  • Molecular prevalence of Tetratrichomonas gallinarum and Trichomonas gallinae in three domestic free-range poultry breeds in Anhui Province, China
    Dong-qian Chen, Xin-yu Luo, Qiao-qiao Li, Jin-chao Pan, Hen Zhang, Yue-yue Gu, Zhen-zhen Kan, Jia-min Huang, Zhui Fang, Xin-chao Liu, You-fang Gu, Wen-Chao Li
    Parasitology Research.2022; 121(10): 2841.     CrossRef
  • Prevalence and diversity of Trichomonas gallinae in meat pigeons (Columba livia) in Guangdong Province, People’s Republic of China
    Haiming Cai, Yu Liu, Yibin Zhu, Zhihong Xu, Qingfeng Zhou, Zhuanqiang Yan, Shenquan Liao, Nanshan Qi, Juan Li, Xuhui Lin, Junjing Hu, Shuilan Yu, Jianfei Zhang, Junwei Lin, Minna Lv, Mingfei Sun
    Parasitology Research.2022; 121(11): 3183.     CrossRef
  • Common bacterial, viral, and parasitic diseases in pigeons (Columba livia): A review of diagnostic and treatment strategies
    Harvey M. Santos, Ching-Yi Tsai, Gail Everette M. Catulin, Kim Chloe G. Trangia, Lemmuel L. Tayo, Hung-Jen Liu, Kuo Pin Chuang
    Veterinary Microbiology.2020; 247: 108779.     CrossRef
  • Prevalence and molecular characterization of Trichomonas gallinae from domestic pigeons in Beijing, China
    Sheng-Yong Feng, Han Chang, Fu-Huang Li, Cheng-Min Wang, Jing Luo, Hong-Xuan He
    Infection, Genetics and Evolution.2018; 65: 369.     CrossRef
  • 8,283 View
  • 169 Download
  • 13 Web of Science
  • Crossref

Case Report

Four Human Cases of Diphyllobothrium nihonkaiense (Eucestoda: Diphyllobothriidae) in China with a Brief Review of Chinese Cases
Yu-Chun Cai, Shao-Hong Chen, Hiroshi Yamasaki, Jia-Xu Chen, Yan Lu, Yong-Nian Zhang, Hao Li, Lin Ai, Hai-Ning Chen
Korean J Parasitol 2017;55(3):319-325.
Published online June 30, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.3.319
We described 4 human infection cases of zoonotic fish-tapeworm, Diphyllobothrium nihonkaiense, identified with morphological and molecular characters and briefly reviewed Chinese cases in consideration of it as an emerging parasitic disease in China. The scolex and mature and gravid proglottids of some cases were seen, a rosette-shaped uterus was observed in the middle of the mature and gravid proglottids, and the diphyllobothriid eggs were yellowish-brown in color and displayed a small knob or abopercular protuberance on the opposite end of a lid-like opening. The average size of the eggs was recorded as 62-67×42-45 μm. The parasitic materials gathered from 4 human cases were morphologically identified as belonging to the genera Diphyllobothrium and Adenocephalus. The phylogenetic analysis based on the nucleotide sequences of cytochrome c oxidase subunit 1 gene of the etiologic agents confirmed that the 4 cases were D. nihonkaiense infection. The finding of 4 additional D. nihonkaiense cases suggests that D. nihonkaiense might be a major causative species of human diphyllobothriasis in China. A combined morphological and molecular analysis is the main method to confirm D. nihonkaiense infection.

Citations

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  • Dietary footprints of a global parasite: diagnosing Dibothriocephalus nihonkaiensis in non-endemic regions
    Wilson G.W. Goh, Jean-Marc Chavatte, Gabriel Z.R. Yan, Yuan Yi Constance Chen, Mark Dhinesh Muthiah, Lionel H.W. Lum
    Gut Pathogens.2025;[Epub]     CrossRef
  • Fish Pathogens and Associated Zoonotic Risks in Aquaculture: A Global One Health Perspective
    Fredrick Juma Syanya, Sajna Beegum, Zachariy Oreko Winam, Fazeena Fariq, A. R. Nikhila Khanna, Megha Lovejan, Mujeeb Rahiman K. M., Harikrishnan Mahadevan
    Aquaculture, Fish and Fisheries.2025;[Epub]     CrossRef
  • Molecular Identification of Dibothriocephalus nihonkaiense Infection Using Nanopore Sequencing: A Case Report and Literature Review
    Hin-Fung Tsang, Stanley W.M. Leung, Tin-Nok Hung, Icy Law, Ka-Wai Lam, Loiston Chan, Sze-Chuen Cesar Wong
    Diagnostics.2024; 14(24): 2871.     CrossRef
  • Human diphyllobothriosis in Taiwan: A review of cases and molecular evidence of Dibothriocephalus nihonkaiensis
    Chia-Kwung Fan, Daniel Barčák, Tomáš Scholz, Pasaikou Sonko, Martina Orosová, Kua-Eyre Su, Chun-Chao Chang, Yuarn-Jang Lee, Roman Kuchta, Mikuláš Oros
    Food and Waterborne Parasitology.2023; 33: e00213.     CrossRef
  • Global analysis of cytochrome c oxidase subunit 1 (cox1) gene variation in Dibothriocephalus nihonkaiensis (Cestoda: Diphyllobothriidae)
    Niichiro Abe, Takashi Baba, Yoshitaka Nakamura, Shintaro Murakami
    Current Research in Parasitology & Vector-Borne Diseases.2021; 1: 100042.     CrossRef
  • Dibothriocephalus nihonkaiensis: an emerging foodborne parasite in Brittany (France)?
    Brice Autier, Sorya Belaz, Brigitte Degeilh, Jean-Pierre Gangneux, Florence Robert-Gangneux
    Parasites & Vectors.2019;[Epub]     CrossRef
  • Epidemiology ofDiphyllobothrium nihonkaienseDiphyllobothriasis, Japan, 2001–2016
    Hiroshi Ikuno, Shinkichi Akao, Hiroshi Yamasaki
    Emerging Infectious Diseases.2018;[Epub]     CrossRef
  • 10,472 View
  • 151 Download
  • 9 Web of Science
  • Crossref

Original Articles

Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran
Meysam Sharifdini, Zahra Heidari, Zahra Hesari, Sajad Vatandoost, Eshrat Beigom Kia
Korean J Parasitol 2017;55(3):279-285.
Published online June 30, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.3.279
The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0-1.8% and 0-0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.

Citations

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  • Helminth Infections in Cattle: A Study on Fecal Samples from the Ağrı Region
    Milad Afşar, Selahattin Aydemir, Sedat Kavak, Sadi Elasan
    Van Veterinary Journal.2025; 36(2): 95.     CrossRef
  • Molecular identification of abomasal nematodes (Haemonchus spp. and Ostertagia ostertagi) in naturally infected calves
    H. H. Shihab, S. D. Hassan
    BULGARIAN JOURNAL OF VETERINARY MEDICINE.2024; 27(2): 286.     CrossRef
  • Trichostrongylosis: a zoonotic disease of small ruminants
    A.H. Bhat, H. Tak, I.M. Malik, B.A. Ganai, N. Zehbi
    Journal of Helminthology.2023;[Epub]     CrossRef
  • Prevalence and Morphometric Comparison of Trichostrongylus spp. among Sheep and Goats from Kashan Abattoir, Central Iran
    Mohsen Arbabi, Aimohammad Bakhshi, Hossein Hooshyar, Reza Ghasemikhah, Mahdi Delavari, Mojtaba Sehat
    Journal of Medical Microbiology and Infectious Diseases.2023; 11(1): 28.     CrossRef
  • Investigating intestinal parasitic infections with emphasis on molecular identification of Strongyloides stercoralis and Trichostrongylus colubriformis in north of Iran
    Fatemeh Hajizadeh, Tahereh Mikaeili Galeh, Seyed Abdollah Hosseini, Seyyed Ali Shariatzadeh, Akram Hematizadeh, Javad Javidnia, Mitra Sadeghi, Mahdi Fakhar, Shirzad Gholami
    Parasite Epidemiology and Control.2023; 22: e00312.     CrossRef
  • Molecular identification of different Trichostrongylus species infecting sheep and goats from Dakahlia governorate, Egypt
    Rana Elseadawy, Ibrahim Abbas, Moustafa Al-Araby, Salah Abu-Elwafa
    Journal of Parasitic Diseases.2021; 45(1): 218.     CrossRef
  • Strongyloides stercoralis and other intestinal parasites in patients receiving immunosuppressive drugs in northern Iran: a closer look at risk factors
    Leila Mirzaei, Keyhan Ashrafi, Zahra Atrkar Roushan, Mohammad Reza Mahmoudi, Irandokht Shenavar Masooleh, Behnaz Rahmati, Farshid Saadat, Hamed Mirjalali, Meysam Sharifdini
    Epidemiology and Health.2021; 43: e2021009.     CrossRef
  • Comparison of Molecular and Parasitological Methods for Diagnosis of Human Trichostrongylosis
    Mehdi Pandi, Meysam Sharifdini, Keyhan Ashrafi, Zahra Atrkar Roushan, Behnaz Rahmati, Nayereh Hajipour
    Frontiers in Cellular and Infection Microbiology.2021;[Epub]     CrossRef
  • Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
    Hedayat Hosseinnezhad, Meysam Sharifdini, Keyhan Ashrafi, Zahra Atrkar Roushan, Hamed Mirjalali, Behnaz Rahmati
    BMC Veterinary Research.2021;[Epub]     CrossRef
  • Prevalence and risk factors of intestinal parasite infections in Mazandaran province, North of Iran
    Zeinab Gholipoor, Hooshang Khazan, Eznoallah Azargashb, Mohammad Reza Youssefi, Ali Rostami
    Clinical Epidemiology and Global Health.2020; 8(1): 17.     CrossRef
  • Zoonotic transmission of Teladorsagia circumcincta and Trichostrongylus species in Guilan province, northern Iran: molecular and morphological characterizations
    Keyhan Ashrafi, Meysam Sharifdini, Zahra Heidari, Behnaz Rahmati, Eshrat Beigom Kia
    BMC Infectious Diseases.2020;[Epub]     CrossRef
  • Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences
    Mohsen Arbabi, Hossein Hooshyar, Majid Lotfinia, Mohamad Ali Bakhshi
    Molecular and Biochemical Parasitology.2020; 236: 111260.     CrossRef
  • Phylogenetic analysis of Trichostrongylus vitrinus isolates from southwest Iran
    Mohammad Amin Ghatee, Seyed Ali Asghar Malek Hosseini, Masoud Marashifard, Mehdi Karamian, Walter Robert Taylor, Ali Jamshidi, Iraj Mobedi, Hasan Azarmehr
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Clinical and haematological characteristics of human trichostrongyliasis
    L. Ghanbarzadeh, M. Saraei, E.B. Kia, F. Amini, M. Sharifdini
    Journal of Helminthology.2019; 93(2): 149.     CrossRef
  • Prevalence and phylogenetic analysis of gastrointestinal helminths (Nematoda: Trichostrongylidae) in ruminant livestock of northwest Iran
    Tahereh BARGHANDAN, Elham HAJİALİLO, Meysam SHARİFDİNİ, Amir JAVADİ
    Ankara Üniversitesi Veteriner Fakültesi Dergisi.2019; 67(1): 65.     CrossRef
  • Gastrointestinal helminths in farmers and their ruminant livestock from the Coastal Savannah zone of Ghana
    Sylvia Afriyie Squire, Rongchang Yang, Ian Robertson, Irene Ayi, Daniel Sai Squire, Una Ryan
    Parasitology Research.2018; 117(10): 3183.     CrossRef
  • Strongyloides stercoralis hyperinfection in an unconscious diabetic patient with dermatomyositis
    Meysam Sharifdini, Aniseh Hesari, SeifAli Mahdavi, Akram Alipour, EshratBeigom Kia
    Indian Journal of Pathology and Microbiology.2018; 61(1): 109.     CrossRef
  • Molecular identification and phylogenetic analysis of human Trichostrongylus species from an endemic area of Iran
    Meysam Sharifdini, Sedigheh Derakhshani, Safar Ali Alizadeh, Laleh Ghanbarzadeh, Hamed Mirjalali, Iraj Mobedi, Mehrzad Saraei
    Acta Tropica.2017; 176: 293.     CrossRef
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  • 223 Download
  • 18 Web of Science
  • Crossref
Complete Mitochondrial Genome of Echinostoma hortense (Digenea: Echinostomatidae)
Ze-Xuan Liu, Yan Zhang, Yu-Ting Liu, Qiao-Cheng Chang, Xin Su, Xue Fu, Dong-Mei Yue, Yuan Gao, Chun-Ren Wang
Korean J Parasitol 2016;54(2):173-179.
Published online April 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.2.173
Echinostoma hortense (Digenea: Echinostomatidae) is one of the intestinal flukes with medical importance in humans. However, the mitochondrial (mt) genome of this fluke has not been known yet. The present study has determined the complete mt genome sequences of E. hortense and assessed the phylogenetic relationships with other digenean species for which the complete mt genome sequences are available in GenBank using concatenated amino acid sequences inferred from 12 protein-coding genes. The mt genome of E. hortense contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 non-coding region. The length of the mt genome of E. hortense was 14,994 bp, which was somewhat smaller than those of other trematode species. Phylogenetic analyses based on concatenated nucleotide sequence datasets for all 12 protein-coding genes using maximum parsimony (MP) method showed that E. hortense and Hypoderaeum conoideum gathered together, and they were closer to each other than to Fasciolidae and other echinostomatid trematodes. The availability of the complete mt genome sequences of E. hortense provides important genetic markers for diagnostics, population genetics, and evolutionary studies of digeneans.

Citations

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  • Genetic variation and population structure of Haemonchus contortus: an in-silico analysis
    W. Wei, Z. Lan, Xuewei Liu, Xinhui Zhang, X. Gu, R. Wang
    Journal of Helminthology.2025;[Epub]     CrossRef
  • The Nuclear Ribosomal Transcription Units of Two Echinostomes and Their Taxonomic Implications for the Family Echinostomatidae
    Yu Cao, Ye Li, Zhong-Yan Gao, Bo-Tao Jiang
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    D. A. Solodovnik, D. M. Atopkin, A. A. Semenchenko, M. Urabe, S. G. Sokolov
    Invertebrate Zoology.2025; 22(3): 411.     CrossRef
  • Complete mitochondrial genome and phylogenetic analysis of Dollfustrema vaneyi (Trematoda: Bucephalidae)
    Ye Hu, Tong Ye, Hong Zou, Gui-Tang Wang, Wen-Xiang Li, Dong Zhang
    BMC Genomics.2024;[Epub]     CrossRef
  • Characterization of the complete mitochondrial genome of Plagiorchis multiglandularis (Digenea, Plagiorchiidae): Comparison with the members of Xiphidiatan species and phylogenetic implications
    Janelle Laura J. Gacad, Natalia I. Yurlova, Natalia M. Ponomareva, Misako Urabe
    Parasitology Research.2023; 122(7): 1545.     CrossRef
  • A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences
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    Journal of Helminthology.2023;[Epub]     CrossRef
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    Xin-ru Guo, Ye Li, Yuan Gao, Yang-yuan Qiu, Zhen-hua Jin, Zhong-yan Gao, Xian-guang Zhang, Qi An, Qiao-cheng Chang, Jun-feng Gao, Chun-ren Wang
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    Y.I. Ivashko, A.A. Semenchenko, D.A. Solodovnik, D.M. Atopkin
    Journal of Helminthology.2022;[Epub]     CrossRef
  • Trematode diversity in freshwater snails from a stopover point for migratory waterfowls in Hokkaido, Japan: An assessment by molecular phylogenetic and population genetic analyses
    Minoru Nakao, Mizuki Sasaki
    Parasitology International.2021; 83: 102329.     CrossRef
  • First next-generation sequencing data for Haploporidae (Digenea: Haploporata): characterization of complete mitochondrial genome and ribosomal operon for Parasaccocoelium mugili Zhukov, 1971
    Dmitry M. Atopkin, Alexander A. Semenchenko, Daria A. Solodovnik, Yana I. Ivashko, Kirill A. Vinnikov
    Parasitology Research.2021; 120(6): 2037.     CrossRef
  • Characterization of the complete mitochondrial genome sequence of Tracheophilus cymbius (Digenea), the first representative from the family Cyclocoelidae
    Y. Li, X.X. Ma, Q.B. Lv, Y. Hu, H.Y. Qiu, Q.C. Chang, C.R. Wang
    Journal of Helminthology.2020;[Epub]     CrossRef
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    Rongkun Ran, Qi Zhao, Asmaa M. I. Abuzeid, Yue Huang, Yunqiu Liu, Yongxiang Sun, Long He, Xiu Li, Jumei Liu, Guoqing Li
    The Korean Journal of Parasitology.2020; 58(1): 73.     CrossRef
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    Yuan-An Wu, Jin-Wei Gao, Xiao-Fei Cheng, Min Xie, Xi-Ping Yuan, Dong Liu, Rui Song
    ZooKeys.2020; 945: 1.     CrossRef
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    Toby Landeryou, Stephen M. Kett, Anne Ropiquet, Dirk Wildeboer, Scott P. Lawton
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    Michelle A. Gordy, Patrick C. Hanington
    Ecology and Evolution.2019; 9(6): 3153.     CrossRef
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    Suleman, Jun Ma, Mian Sayed Khan, Vasyl V. Tkach, Nehaz Muhammad, Dong Zhang, Xing-Quan Zhu
    Parasitology International.2019; 71: 99.     CrossRef
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    Jun Ma, Jun-Jun He, Cheng-Yan Zhou, Miao-Miao Sun, William Cevallos, Hiromu Sugiyama, Xing-Quan Zhu, Manuel Calvopiña
    Acta Tropica.2019; 195: 90.     CrossRef
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    Suleman, Mian Sayed Khan, Petr Heneberg, Cheng-Yan Zhou, Nehaz Muhammad, Xing-Quan Zhu, Jun Ma
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    Ye Li, Yang-Yuan Qiu, Min-Hao Zeng, Pei-Wen Diao, Qiao-Cheng Chang, Yuan Gao, Yan Zhang, Chun-Ren Wang
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    Yuan Gao, Yan Zhang, Xin Yang, Jian-Hua Qiu, Hong Duan, Wen-Wen Xu, Qiao-Cheng Chang, Chun-Ren Wang
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  • 22 Web of Science
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Brief Communications

Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
Giyong Seong, Yu-Jung Han, Jeong- Byoung Chae, Joon-Seok Chae, Do-Hyeon Yu, Young-Sung Lee, Jinho Park, Bae-Keun Park, Jae-Gyu Yoo, Kyoung-Seong Choi
Korean J Parasitol 2015;53(6):765-769.
Published online December 31, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.6.765
Anaplasma species are obligate intracellular pathogens that can cause tick-borne diseases in mammalian hosts. To date, very few studies of their occurrence in Korean native goats (Capra aegagrus hircus) have been reported. In the present study, we investigated Anaplasma infection of Korean native goats on Jeju Island, Republic of Korea, and performed phylogenetic analysis based on the 16S rRNA gene sequences. Our results showed that Anaplasma infection was found mostly in adult female goats. The phylogenetic tree revealed that the 7 sequences identified in Korean native goats could belong to Anaplasma sp. and were distinct from A. marginale, A. centrale, and A. ovis. The results indicated that the sequences identified to belong to Anaplasma were closely related to sequences isolated from goats in China and were clustered within the same group. To our knowledge, this is the first study to detect Anaplasma sp. infection in Korean native goats.

Citations

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    Juan A. Segura, Antonia Dibernardo, Kathy Manguiat, Brooks Waitt, Zulma V. Rueda, Yoav Keynan, Heidi Wood, Lina A. Gutiérrez
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  • Molecular Detection and Phylogenetic Analysis ofAnaplasmaspp. in Korean Native Goats from Ulsan Metropolitan City, Korea
    Hyun-Ji Seo, Byung-Chan Jin, Keun-Ho Kim, Mi-Sun Yoo, Kwang-Won Seong, Seong-Jin Jeong, Bang-Hun Hyun, Yun Sang Cho
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  • Molecular Detection of Anaplasma, Bartonella, and Borrelia theileri in Raccoon Dogs (Nyctereutes procyonoides) in Korea
    Hang Lee, Joon-Seok Chae, Bae-Keun Park, Jinho Park, Do-Hyeon Yu, Jun-Gu Kang, Nam-Shik Shin, Young-Sun Jo, Yoon-Kyoung Cho, Kyoung-Seong Choi, Jeong-Byoung Chae
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  • Molecular identification of selected tick-borne pathogens in wild deer and raccoon dogs from the Republic of Korea
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    Veterinary Parasitology: Regional Studies and Reports.2017; 7: 25.     CrossRef
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Genetic Diversity of Toxoplasma gondii Strains from Different Hosts and Geographical Regions by Sequence Analysis of GRA20 Gene
Hong-Rui Ning, Si-Yang Huang, Jin-Lei Wang, Qian-Ming Xu, Xing-Quan Zhu
Korean J Parasitol 2015;53(3):345-348.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.345
Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.

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  • Effects of latent infection of Toxoplasma gondii strains with different genotypes on mouse behavior and brain transcripts
    Bei-Bei Zhou, Hong-Jie Dong, Hang Sun, Xiao-Man Xie, Huan-Huan Xie, Wen-Ju Zhu, Ya-Nan Li, Chao Xu, Jian-Ping Cao, Gui-Hua Zhao, Kun Yin
    Parasites & Vectors.2025;[Epub]     CrossRef
  • First Report on the Molecular Detection and Genetic Characterization of Toxoplasma gondii From Donkeys in Kenya
    Fredrick O. Obonyo, Ndichu Maingi, Samuel M. Githigia, Kevin O. Ochwedo, Anne A. Owiti, Evans N. Nyaboga
    Acta Parasitologica.2024; 69(3): 1480.     CrossRef
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  • 2 Web of Science
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Original Articles
Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions
Shuai Wang, Aiping Cao, Xun Li, Qunli Zhao, Yuan Liu, Hua Cong, Shenyi He, Huaiyu Zhou
Korean J Parasitol 2015;53(3):253-258.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.253
Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.

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  • Superoxide Dismutases in Immune Regulation and Infectious Diseases
    Tong Liu, Jiajin Shang, Qijun Chen
    Antioxidants.2025; 14(7): 809.     CrossRef
  • Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase (TgSOD) induces partial immune protection against acute toxoplasmosis in BALB/c mice
    Yuan Liu, Aiping Cao, Yawen Li, Xun Li, Hua Cong, Shenyi He, Huaiyu Zhou
    BMC Infectious Diseases.2017;[Epub]     CrossRef
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Developmental and Phylogenetic Characteristics of Stellantchasmus falcatus (Trematoda: Heterophyidae) from Thailand
Pralongyut Sripalwit, Chalobol Wongsawad, Thapana Chontananarth, Somboon Anuntalabhochai, Pheravut Wongsawad, Jong-Yil Chai
Korean J Parasitol 2015;53(2):201-207.
Published online April 22, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.2.201
This study aimed to investigate the infection status, worm development, and phylogenetic characteristics of the intestinal trematode, Stellantchasmus falcatus. The metacercariae of S. falcatus were detected only in the half-beak (Dermogenus pusillus) out of the 4 fish species examined. Their prevalence was 90.0%, and the intensity of infection was 919 metacercariae on average. Worms were recovered from 33 (97.1%) of 34 chicks that were experimentally infected with 200 S. falcatus metacercariae each, and the average recovery rate was 43.0%. The body size and inner organs of S. falcatus quickly increased in the experimental chicks over days 1-2 post-infection (PI). In addition, ITS2 sequence data of this parasite were analyzed to examine the phylogenetic relationships with other trematodes using the UPGMA method. The results indicated that the ITS2 sequence data recorded from trematodes in the family Heterophyidae appeared to be monophyletic. This study concluded that D. pusillus serves as a compatible second intermediate host of S. falcatus in Thailand and that S. falcatus can develop rapidly in the experimental chicks. Data collected from this study can help to close the gap in knowledge regarding the epidemiology, biology, and phylogenetic characteristics of S. falcatus in Thailand.

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    Laddawan Patarwut, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
    The Korean Journal of Parasitology.2020; 58(1): 27.     CrossRef
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    Chalobol Wongsawad, Nattawadee Nantarat, Pheravut Wongsawad, Preeyaporn Butboonchoo, Jong-Yil Chai
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PCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea in the Republic of Korea
Kim, Yun Ah , Kim, Hye Youn , Cho, Shin Hyeong , Cheun, Hyeong Il , Yu, Jae Ran , Lee, Sang Eun
Korean J Parasitol 2010;48(1):9-13.
DOI: https://doi.org/10.3347/kjp.2010.48.1.9
Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce diarrhea, have been attributed to opportunistic overgrowth of P. hominis. This study was performed to confirm the P. hominis infection and its molecular characterization from the feces of puppies with diarrhea. Fecal samples were obtained from 14 German shepherd puppies with diarrhea over 1 week (7 females and 7 males, 2-9 months of age) residing on a dog farm in August 2007. Species-specific PCR assay identified P. hominis 18S rRNA genes in 3 of the 14 puppies (1 female and 2 males; 1 aged 2 months and 2 aged 9 months). This phylogenetic analysis established that P. hominis belonged to the 1st clade, which is comprised of Bos taurus and Felines.

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