Warning: fopen(/home/virtual/parasitol/journal/upload/ip_log/ip_log_2025-12.txt): failed to open stream: Permission denied in /home/virtual/lib/view_data.php on line 83
Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 A cysteine protease of Paragonimus westermani eggs
Protease activity was identified in crude extracts of Paragonimus westermani eggs which were purified from infected dog lungs, isolated on 14 weeks after metacercarial challenge. The eggs were used after removing possibly contaminated host or worm tissues on their shell surfaces.
In the crude egg extracts, high proteolytic activities against carboxybenzoyl-phenylalanyl-arginyl-4-methoxy-beta- naphthylamide (Cbz-phe-arg-MNA) and Azocoll were detected whereas those against succinyl-alanyl-prolyl- phenylalanyl-p-nitroanilide (Suc-ala- pro-phe-pNA) were not revealed. The enzyme exhibited the maximal activity at pH 6.
Its activity was inhibited by specific cysteine protease inhibitors, 10(-5) M 1-trans-epoxysuccinylleucylamido (4- guanidino) butane (E-64) and 1 mM iodoacetamide (IAA) while potentiated by 6.5-fold in the presence of 2.5 mM dithiothreitol (DTT). When the enzyme was purified partially by Sephacryl S-300 High Resolution gel filtration, it migrated as a single homogeneous band at 35 kDa. The 35 kDa cysteine protease has been recognized neither in the metacercariae nor in the adult. These findings indicated the presence of at least one protease of cathepsin family in immature eggs of P. westermani.
Citations
Citations to this article as recorded by
Expression characteristics and specific antibody reactivity of diverse cathepsin F members of Paragonimus westermani Chun-Seob Ahn, Byoung-Kuk Na, Dong-ll Chung, Jeong-Geun Kim, Jin-Taek Kim, Yoon Kong Parasitology International.2015; 64(1): 37. CrossRef
Identification of immunodominant excretory–secretory cysteine proteases of adult Paragonimus westermani by proteome analysis Eung‐Goo Lee, Byoung‐Kuk Na, Young‐An Bae, Seon‐Hee Kim, Eun‐Young Je, Jeong‐Won Ju, Shin‐Hyeong Cho, Tong‐Soo Kim, Shin‐Yong Kang, Seung‐Yull Cho, Yoon Kong PROTEOMICS.2006; 6(4): 1290. CrossRef
COMPARISON OF GENE REPRESENTATION BETWEEN DIPLOID AND TRIPLOID PARAGONIMUS WESTERMANI BY EXPRESSED SEQUENCE TAG ANALYSES Tae-Suk Kim, Jefferson V. de Guzman, Hyun-Hee Kong, Dong-Il Chung Journal of Parasitology.2006; 92(4): 803. CrossRef
Molecular Expression of a Cysteine Proteinase of
Clonorchis sinensis
and Its Application to an Enzyme-Linked Immunosorbent Assay for Immunodiagnosis of Clonorchiasis
Isao Nagano, Fuquan Pei, Zhiliang Wu, Jun Wu, Huier Cui, Thidarut Boonmars, Yuzo Takahashi Clinical and Vaccine Immunology.2004; 11(2): 411. CrossRef
Cystatin Capture Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Clonorchiasis and Profile of Captured Antigenic Protein ofClonorchis sinensis Tae Yun Kim, Shin-Yong Kang, Sun Hyo Park, Kom Sukontason, Kabkaew Sukontason, Sung-Jong Hong Clinical Diagnostic Laboratory Immunology.2001; 8(6): 1076. CrossRef
Persisting antibody reaction in paragonimiasis after praziquantel treatment is elicited mainly by egg antigens Seung-Yull Cho, Yoon Kong, Doo-Hee Yun, Shin-Yong KanG, Lee-Soo Kim, Young-Bae Chung, Hyun-Jong Yang The Korean Journal of Parasitology.2000; 38(2): 75. CrossRef
Structural and Immunological Characteristics of a 28-Kilodalton Cruzipain-Like Cysteine Protease ofParagonimus westermaniExpressed in the Definitive Host Stage Doo-Hee Yun, Joon-Yong Chung, Young-Bae Chung, Young-Yil Bahk, Shin-Yong Kang, Yoon Kong, Seung-Yull Cho Clinical Diagnostic Laboratory Immunology.2000; 7(6): 932. CrossRef
Immunoglobulin G (IgG) Subclass and IgE Responses in Human Paragonimiases Caused by Three Different Species Yoon Kong, Akira Ito, Hyun-Jong Yang, Young-Bae Chung, Shiro Kasuya, Masashi Kobayashi, Yue-Han Liu, Seung-Yull Cho Clinical Diagnostic Laboratory Immunology.1998; 5(4): 474. CrossRef
Activities of different cysteine proteases of Paragonimus westermani in cleaving human IgG Y B Chung, H J Yang, S Y Kang, Y Kong, S Y Cho The Korean Journal of Parasitology.1997; 35(2): 139. CrossRef
Variation of antigenic proteins of eggs and developmental stages of Paragonimus westermani Y Kong, J Y Chung, D H Yun, L S Kim, S Y Kang, A Ito, L Ma, S Y Cho The Korean Journal of Parasitology.1997; 35(3): 197. CrossRef
