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Brief Communication

Molecular characterization of tick genera Haemaphysalis, Ixodes, and Amblyomma in Korea
Subin Lee, Badriah Alkathiri, Kyu-Sung Ahn, Jiwon Kim, So Youn Youn, Mi-Sun Yoo, Hyang-Sim Lee, Jae-Myung Kim, Dongmi Kwak, Sung Shik Shin, Seung-Hun Lee
Parasites Hosts Dis 2025;63(3):272-277.
Published online August 20, 2025
DOI: https://doi.org/10.3347/PHD.25035
Ticks are important ectoparasites that serve as key vectors of various pathogens, posing significant risks to both human and animal health. In this study, 3 genera and 5 species of ticks, including Haemaphysalis longicornis, H. flava, H. doenitzi, Ixodes nipponensis, and Amblyomma testudinarium, were analyzed both morphologically and molecularly. Morphological identification was conducted using established taxonomic keys, followed by molecular characterization through analysis of complete cytochrome c oxidase subunit 1 (cox1, 1,539 bp) and mitochondrial 16S rDNA (1,191–1,215 bp), and nuclear 18S rDNA (1,812–1,816 bp). Phylogenetic and pairwise distance analyses demonstrated that all 3 genes were effective for genus identification; cox1 and mitochondrial 16S rDNA were more effective than 18S rDNA in species identification. Additionally, this study is the first to identify H. doenitzi in the Korean mainland via molecular characterization. These results may serve as reference data for the molecular identification of tick species.
  • 1,714 View
  • 39 Download

Review

Paleoparasitology research on ancient helminth eggs and larvae in the Republic of Korea
Jong-Yil Chai, Min Seo, Dong Hoon Shin
Parasites Hosts Dis 2023;61(4):345-387.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23085
Paleoparasitology is a discipline that applies existing conventional and molecular techniques to study parasites found in ancient ruins. This review focuses on the history of the discovery of parasites (mostly helminth eggs and larvae) in archaeological soil samples and mummies in Korea from the Three Kingdoms Period to the Joseon Dynasty (100 BCE-1910 CE). We also briefly review important milestones in global paleoparasitology. The helminth species reported so far in Korea included Ascaris lumbricoides, Trichuris trichiura, Strongyloides stercoralis (larva), Trichostrongylus sp. (larva), Paracapillaria philippinensis (syn. Capillaria philippinensis), Enterobius vermicularis, Fasciola hepatica, dicrocoeliids, Paragonimus westermani, Clonorchis sinensis, Metagonimus yokogawai, Pygidiopsis summa, Gymnophalloides seoi, Isthmiophora hortensis, Dibothriocephalus nihonkaiensis (syn. Diphyllobothrium nihonkaiense), and Taenia spp. tapeworms. The findings obtained by Korean paleoparasitologists/archaeologists have brought about deep insight into the status of helminthic infections in Korea’s past populations. Continued paleoparasitological research is essential for further understanding of ancient parasites and parasitic diseases in Korea.

Citations

Citations to this article as recorded by  Crossref logo
  • Imported parasitic diseases in the Republic of Korea: status and issues
    Jong-Yil Chai
    Journal of the Korean Medical Association.2025; 68(1): 52.     CrossRef
  • 7,164 View
  • 224 Download
  • 1 Web of Science
  • Crossref

Mini Reviews

Recent progress in vaccine development targeting pre-clinical human toxoplasmosis
Ki-Back Chu, Fu-Shi Quan
Parasites Hosts Dis 2023;61(3):231-239.
Published online August 21, 2023
DOI: https://doi.org/10.3347/PHD.22097
Toxoplasma gondii is an intracellular parasitic organism affecting all warm-blooded vertebrates. Due to the unavailability of commercialized human T. gondii vaccine, many studies have been reported investigating the protective efficacy of pre-clinical T. gondii vaccines expressing diverse antigens. Careful antigen selection and implementing multifarious immunization strategies could enhance protection against toxoplasmosis in animal models. Although none of the available vaccines could remove the tissue-dwelling parasites from the host organism, findings from these pre-clinical toxoplasmosis vaccine studies highlighted their developmental potential and provided insights into rational vaccine design. We herein explored the progress of T. gondii vaccine development using DNA, protein subunit, and virus-like particle vaccine platforms. Specifically, we summarized the findings from the pre-clinical toxoplasmosis vaccine studies involving T. gondii challenge infection in mice published in the past 5 years.

Citations

Citations to this article as recorded by  Crossref logo
  • PLGA nanoparticles as an efficient carrier in Toxoplasma GAP45: a more effective vaccine against acute toxoplasmosis than traditional ones
    Pan Zhou, YanLi Yu, WeiYu Qi, XiaoJuan Wang, YouLi Yu, JianDong Wang, Li Zhang, ZhengQing Yu, TingLi Liu
    Frontiers in Immunology.2025;[Epub]     CrossRef
  • Influenza virus-like particles presenting Toxoplasma gondii dense granule protein 7 protect mice from lethal ME49 challenge
    Jie Mao, Hae-Ji Kang, Su-In Heo, Fu-Shi Quan
    Nanomedicine.2025; 20(18): 2309.     CrossRef
  • Recombinant TgDDX3X DEAD-box protein confers partial protection in murine models of acute and chronic toxoplasmosis
    Shuai Wang, Jinghui Wang, Youke Fan, Haina Zhang, Junru Wu, Tingting Ying, Hangbin Ma, Qiangqiang Wang, Longkang Wang, Yuanfeng Wang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang, Zhenke Yang
    Acta Tropica.2025; 270: 107780.     CrossRef
  • Vaccinia virus expressing MIC8 and AMA1 provides protection against Toxoplasma gondii ME49 infection
    Hae-Ji Kang, Yan Jin, Zhao-Shou Yang, Md Atique Ahmed, Fu-Shi Quan
    Parasites, Hosts and Diseases.2025; 63(4): 340.     CrossRef
  • Evaluation of protective efficacy of recombinant Toxoplasma gondii DDX39 protein vaccine against acute and chronic T. gondii infection in mice
    Jinghui Wang, Yuanfeng Wang, Haina Zhang, Hangbin Ma, Qiangqiang Wang, Longkang Wang, Youke Fan, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang, Shuai Wang, Zhenke Yang
    Acta Tropica.2024; 260: 107442.     CrossRef
  • 8,232 View
  • 139 Download
  • 4 Web of Science
  • Crossref
Leishmania Vaccines: the Current Situation with Its Promising Aspect for the Future
Rasit Dinc
Korean J Parasitol 2022;60(6):379-391.
Published online December 22, 2022
DOI: https://doi.org/10.3347/kjp.2022.60.6.379
Leishmaniasis is a serious parasitic disease caused by Leishmania spp. transmitted through sandfly bites. This disease is a major public health concern worldwide. It can occur in 3 different clinical forms: cutaneous, mucocutaneous, and visceral Leishmaniasis (CL, MCL, and VL, respectively), caused by different Leishmania spp. Currently, licensed vaccines are unavailable for the treatment of human Leishmaniasis. The treatment and prevention of this disease rely mainly on chemotherapeutics, which are highly toxic and have an increasing resistance problem. The development of a safe, effective, and affordable vaccine for all forms of vector-borne disease is urgently needed to block transmission of the parasite between the host and vector. Immunological mechanisms in the pathogenesis of Leishmaniasis are complex. IL-12-driven Th1-type immune response plays a crucial role in host protection. The essential purpose of vaccination is to establish a protective immune response. To date, numerous vaccine studies have been conducted using live/attenuated/killed parasites, fractionated parasites, subunits, recombinant or DNA technology, delivery systems, and chimeric peptides. Most of these studies were limited to animals. In addition, standardization has not been achieved in these studies due to the differences in the virulence dynamics of the Leishmania spp. and the feasibility of the adjuvants. More studies are needed to develop a safe and effective vaccine, which is the most promising approach against Leishmania infection.

Citations

Citations to this article as recorded by  Crossref logo
  • Identification of novel anti‐leishmanials targeting glutathione synthetase of the parasite: a drug repurposing approach
    Manash Sarma, Kushal Bora, Preeti Ranjan, Vikash Kumar Dubey
    FEBS Letters.2025; 599(3): 367.     CrossRef
  • Molecular Mechanisms of Cell Death in Leishmania donovani Induced by Selected Steroidal Alkaloids
    Naveena Menpadi, Pranjal Chandra, Vikash Kumar Dubey
    Journal of Basic Microbiology.2025;[Epub]     CrossRef
  • Key Facets for the Elimination of Vector-Borne Diseases Filariasis, Leishmaniasis, and Malaria
    Rini Chaturvedi, Amit Sharma
    ACS Infectious Diseases.2025; 11(2): 287.     CrossRef
  • Human migrations, anthropogenic changes, and insect-borne diseases in Latin America
    André B. B. Wilke, Priscilla Farina, Marco Ajelli, Angelo Canale, Filipe Dantas-Torres, Domenico Otranto, Giovanni Benelli
    Parasites & Vectors.2025;[Epub]     CrossRef
  • Vaccines in Dermatology—Present and Future: A Review
    Eyan Goh, Jean-Marc Chavatte, Raymond T. P. Lin, Lisa F. P. Ng, Laurent Rénia, Hazel H. Oon
    Vaccines.2025; 13(2): 125.     CrossRef
  • Structure‐based Virtual Screening and Drug Design Development of Leishmanicidal Pyrimidines
    Gerliny Bezerra de Oliveira, Érick Caique Santos Costa, Zenaide Severina do Monte, Gleybson Correia de Almeida, Emerson Peter da Silva Falcão, Luciana Scotti, Marcus Tullius Scotti, Ricardo Oliveira Silva, Daniele Santana de Sousa Oliveira, Policarpo Adem
    Chemistry & Biodiversity.2025;[Epub]     CrossRef
  • Attitudes and acceptance of vaccination against neglected tropical diseases: A multi-country study in Asia
    Li Ping Wong, Hai Yen Lee, Haridah Alias, Farhana Nishat Seheli, Abhishek Lachyan, Di Khanh Nguyen, Jamil Ahmed, Zhijian Hu, Yulan Lin
    Human Vaccines & Immunotherapeutics.2025;[Epub]     CrossRef
  • Vaccine Designing Technology against Leishmaniasis: Current Challenges and Implication
    Jyoti Gupta, Yukta Menon, Subodh Kumar, Chakresh Kumar Jain
    Current Drug Discovery Technologies.2025;[Epub]     CrossRef
  • Cutaneous Leishmaniasis in the Context of Global Travel, Migration, Refugee Populations, and Humanitarian Crises
    Janice Kim, Tarek Zieneldien, Sophia Ma, Bernard A. Cohen
    Clinics and Practice.2025; 15(4): 77.     CrossRef
  • Histone Deacetylase Inhibitors Show a Potential Leishmanicidal Effect against Leishmania braziliensis in a Mouse Infection Model and Lead to Less Toxicity than Glucantime
    Luciana Ângelo de Souza, Lethícia Kelly Ramos Andrade, Joice de Melo Agripino, Victor Hugo Ferraz da Silva, Sabrina de Oliveira Emerick, Adriana Carneiro da Silva, Larissa Coelho Pereira, Graziela Domingues de Almeida Lima, Ingrid Rabite Garcia, Anna Cláu
    ACS Omega.2025; 10(19): 19466.     CrossRef
  • Leishmania regulates host YY1: Comparative proteomic analysis identifies infection modulated YY1 dependent proteins
    Harsimran Kaur Brar, Eleanor Chen, Fabian Chang, Shawna Angel Lu, Dilraj Kaur Longowal, Kyung-Mee Moon, Leonard J. Foster, Neil Reiner, Devki Nandan, Filomena de Nigris
    PLOS One.2025; 20(5): e0323227.     CrossRef
  • Computational vaccine development against protozoa
    Omar Hashim, Isabelle Dimier-Poisson
    Computational and Structural Biotechnology Journal.2025; 27: 2386.     CrossRef
  • Control measures for neglected tropical diseases: vaccine updates
    Vivek P. Chavda, Suneetha Vuppu, Toshika Mishra, Nikita Sharma, Sathvika Kamaraj, Shatakshi Mishra, Bhumi Sureshbhai, John Matsoukas, Vasso Apostolopoulos
    Expert Review of Vaccines.2025; 24(1): 535.     CrossRef
  • Attitudes and acceptance of mRNA-based vaccine for neglected tropical diseases: A multi-country study in Asia
    Li Ping Wong, Hai Yen Lee, Haridah Alias, Di Khanh Nguyen, Abhishek Lachyan, Farhana Nishat Seheli, Jamil Ahmed, Zhijian Hu, Yulan Lin
    Human Vaccines & Immunotherapeutics.2025;[Epub]     CrossRef
  • Development of vaccines against Leishmania mexicana: a reverse vaccinology approach
    Isis Pérez-Concepción, Iván Corona Guerrero, Angela Corina Hayano Kanashiro, Nohemí Gámez Meza, Armando Tejeda-Mansir
    Biotecnia.2025; 27: e2537.     CrossRef
  • A Human Monoclonal Antibody Targeting α-Gal Ameliorates Experimental Cutaneous Leishmaniasis
    Hyeseon Cho, Andrea Paun, Youngsil Seo, Sang Hun Lee, Maria Traver, Ming Zhao, Jeff Skinner, Joshua Tan, David L Sacks, Peter D Crompton
    The Journal of Infectious Diseases.2025;[Epub]     CrossRef
  • Advances in recombinant protein vaccines against Leishmania infantum: a systematic review of vaccinal constructs and delivery strategies
    André Zaidan Martins, Ana Laura Grossi de Oliveira, Lilian Lacerda Bueno, Fabrício Marcus Silva Oliveira, Ricardo Toshio Fujiwara
    Acta Tropica.2025; 270: 107796.     CrossRef
  • The impact of probiotic administration in vivo on peritoneal mouse macrophages infected by Leishmania amazonensis ex vivo
    Lauren Van den Broeck, Raquel Silva de Azevedo, Ludmila Ferreira de Almeida Fiuza, Marcos Meuser Batista, Cynthia Machado Cascabulho, Ewout Van de Velde, Serge Van Calenbergh, Guy Caljon, Maria de Nazaré Correia Soeiro
    Memórias do Instituto Oswaldo Cruz.2025;[Epub]     CrossRef
  • In silico design and assessment of a multiepitope and multiantigen potential vaccine candidate against Leishmania donovani
    Satabdi Saha, Sahina Laskar, Seshan Gunalan, Gugan Kothandan, Diwakar Kumar
    In Silico Pharmacology.2025;[Epub]     CrossRef
  • Understanding Sex-biases in Kinetoplastid Infections: Leishmaniasis and Trypanosomiasis
    Olivia Battistoni, Ryan H. Huston, Chaitenya Verma, Thalia Pacheco-Fernandez, Sara Abul-Khoudoud, Alison Campbell, Abhay R. Satoskar
    Expert Reviews in Molecular Medicine.2025;[Epub]     CrossRef
  • Luring the vector: A systematic review of sand fly attractants
    Panagiota Tsafrakidou, Arsen Gkektsian, Michael Miaoulis, Lee W. Cohnstaedt, Alexandra Chaskopoulou
    Current Research in Parasitology & Vector-Borne Diseases.2025; 8: 100325.     CrossRef
  • Intracellular Parasitic Infections Caused by Plasmodium falciparum, Leishmania spp., Toxoplasma gondii, Echinococcus multilocularis, Among Key Pathogens: Global Burden, Transmission Dynamics, and Vaccine Advances—A Narrative Review with Contextual Insight
    Tatevik Sargsyan, Lala Stepanyan, Avetis Tsaturyan, Rosanna Palumbo, Caterina Vicidomini, Giovanni N. Roviello
    Vaccines.2025; 13(11): 1082.     CrossRef
  • Repurposing Acetylcholinesterase Inhibitors for Leishmaniasis: Donepezil Hydrochloride and Related Compounds Against the American Tegumentary Form
    Daniela E. Barraza, Emilse N. Araoz, María A. Occhionero, Daniela A. Gaspar, Eliana G. Guevara, María E. Vázquez, Brenda A. Zabala, Paola A. Barroso, Cecilia Pérez Brandán, Carlos J. Minahk, Leonardo Acuña
    Antibiotics.2025; 14(12): 1182.     CrossRef
  • Genetic Manipulation Tools in Leishmania: From CRISPR/Cas9 to Vaccine Strategies for Disease Control
    Merve Gundogdu, Zeynep Islek
    Acta Parasitologica.2025;[Epub]     CrossRef
  • Cutaneous leishmaniasis: immunological insights and clinical challenges
    Héctor Serrano-Coll, Lucero Katherine Aristizábal Parra, Graciela Olarte, Carolina Salamanca-Leguizamón
    Colombia Medica.2025; 56(3): e3006750.     CrossRef
  • CRISPR-Cas13b mediated gene knockdowns in Leishmania infantum
    Marine Queffeulou, Raouia Fakhfakh, Fereshteh Fani, Alex Dos Santos, Gabriel Reis Ferreira, Sophia Bigot, Chantal Godin, Philippe Leprohon, Barbara Papadopoulou, Marc Ouellette
    International Journal for Parasitology: Drugs and Drug Resistance.2025; : 100629.     CrossRef
  • Memory T cells: promising biomarkers for evaluating protection and vaccine efficacy against leishmaniasis
    Mahmoud Nateghi-Rostami, Yahya Sohrabi
    Frontiers in Immunology.2024;[Epub]     CrossRef
  • GSK-3 kinase a putative therapeutic target in trypanosomatid parasites
    Alfredo Prado Diaz, Cristian Alejandro Meneses Canal, Alvaro José Valdés, Jaider Elian Giraldo Delgado, RE Varela-M
    The Brazilian Journal of Infectious Diseases.2024; 28(2): 103736.     CrossRef
  • Live attenuated-nonpathogenic Leishmania and DNA structures as promising vaccine platforms against leishmaniasis: innovations can make waves
    Negar Seyed, Tahereh Taheri, Sima Rafati
    Frontiers in Microbiology.2024;[Epub]     CrossRef
  • Dual Antitubercular and Antileishmanial Profiles of Quinoxaline Di-N-Oxides Containing an Amino Acidic Side Chain
    Juan F. González, María-Auxiliadora Dea-Ayuela, Lena Huck, José María Orduña, Francisco Bolás-Fernández, Elena de la Cuesta, Nazia Haseen, Ashraf Ali Mohammed, J. Carlos Menéndez
    Pharmaceuticals.2024; 17(4): 487.     CrossRef
  • The role of CD4+ T cells in visceral leishmaniasis; new and emerging roles for NKG7 and TGFβ
    Jinrui Na, Christian Engwerda
    Frontiers in Cellular and Infection Microbiology.2024;[Epub]     CrossRef
  • Pilot-Scale Screening of Clinically Approved Drugs to Identify Uridine Insertion/Deletion RNA Editing Inhibitors in Trypanosoma brucei
    Mojtaba Rostamighadi, Arezou Kamelshahroudi, Vanessa Pitsitikas, Kenneth A. Jacobson, Reza Salavati
    ACS Infectious Diseases.2024; 10(9): 3289.     CrossRef
  • Effect of Leishmania infantum infection on B cell lymphopoiesis and memory in the bone marrow and spleen
    Laura Dirkx, Marlotte Loyens, Sara I. Van Acker, Dimitri Bulté, Mathieu Claes, Magdalena Radwanska, Stefan Magez, Guy Caljon
    The FASEB Journal.2024;[Epub]     CrossRef
  • In silico and in vitro evaluation of the immunogenic potential of Leishmania donovani ascorbate peroxidase and its derived peptides
    Shobha Kumari, Saravanan Vijaykumar, Vikash Kumar, Ravi Ranjan, Dayakar Alti, Veer Singh, Ghufran Ahmed, Ganesh Chandra Sahoo, Krishna Pandey, Ashish Kumar
    Acta Tropica.2024; 260: 107381.     CrossRef
  • The Major Role of T Regulatory Cells in the Efficiency of Vaccination in General and Immunocompromised Populations: A Review
    Stanislaw Stepkowski, Dulat Bekbolsynov, Jared Oenick, Surina Brar, Beata Mierzejewska, Michael A. Rees, Obi Ekwenna
    Vaccines.2024; 12(9): 992.     CrossRef
  • Prospects and Challenges of Genetically Modified Live-Attenuated Leishmania Vaccines
    Suman Karmakar, Mousumi Das, Monalisa Ray, Soumyadip Mukherjee, Sanhita Ghosh, Kamalika Roy, Chiranjib Pal
    Zoonoses.2024;[Epub]     CrossRef
  • A review of leishmaniasis: current knowledge and future directions of heterocyclic molecules
    Tejaswini Masne, Dileep Kumar, Deepali Bansode
    Exploration of Drug Science.2024; : 508.     CrossRef
  • A squalene oil emulsified MPL-A and anti-CD200/CD300a antibodies adjuvanted whole-killed Leishmania vaccine provides durable immunity against L. donovani parasites
    Baishakhi Mahapatra, Abhishek Singh, Arpita Banerjee, Shruti Sirohi, Samer Singh, Vikash K. Dubey, Rakesh K. Singh
    Vaccine.2024; 42(26): 126373.     CrossRef
  • C57BL/6 Peritoneal Macrophage Exosomes Improve Antileishmanial Functions of the RAW264.7 Cells
    Hadis Gandomkar, Mostafa Changaei, Mir Mohammadreza Hosseini, Sara Soudi, Ahmad Zavaran Hosseini
    Parasite Immunology.2024;[Epub]     CrossRef
  • India should invest in the expansion of genomic epidemiology for vector-borne diseases filariasis, malaria and visceral leishmaniasis that are targeted for elimination
    Nandini Singh, Amit Sharma
    IJID Regions.2024; 13: 100453.     CrossRef
  • Advances in Leishmania Vaccines: Current Development and Future Prospects
    Andreina Ayala, Alejandro Llanes, Ricardo Lleonart, Carlos M. Restrepo
    Pathogens.2024; 13(9): 812.     CrossRef
  • Development and application of species ID and insecticide resistance assays, for monitoring sand fly Leishmania vectors in the Mediterranean basin and in the Middle East
    Sofia Balaska, Jahangir Khajehali, Konstantinos Mavridis, Mustafa Akiner, Kyriaki Maria Papapostolou, Latifa Remadi, Ilias Kioulos, Michail Miaoulis, Emmanouil Alexandros Fotakis, Alexandra Chaskopoulou, John Vontas, Yara M. Traub-Csekö
    PLOS Neglected Tropical Diseases.2024; 18(12): e0012408.     CrossRef
  • A Review of the Progress in the Development of Leishmaniasis Vaccines
    Narges Khaghanzadeh, Fatemeh Javadi, Afshin Samiei
    Journal of Ardabil University of Medical Sciences.2024; 24(2): 129.     CrossRef
  • A minor emphasis on the outbreak of cutaneous leishmaniasis after devastating earthquakes in Turkey
    Ilknur Nihal Ardic, Nurittin Ardic
    Asian Pacific Journal of Tropical Medicine.2023; 16(3): 97.     CrossRef
  • Immunotherapy and immunochemotherapy in combating visceral leishmaniasis
    Ganesh Yadagiri, Aakriti Singh, Kanika Arora, Shyam Lal Mudavath
    Frontiers in Medicine.2023;[Epub]     CrossRef
  • Efficacy of an Immunotherapy Combining Immunogenic Chimeric Protein Plus Adjuvant and Amphotericin B against Murine Visceral Leishmaniasis
    Danniele L. Vale, Camila S. Freitas, Vívian T. Martins, Gabriel J. L. Moreira, Amanda S. Machado, Fernanda F. Ramos, Isabela A. G. Pereira, Raquel S. Bandeira, Marcelo M. de Jesus, Grasiele S. V. Tavares, Fernanda Ludolf, Miguel A. Chávez-Fumagalli, Alexs
    Biology.2023; 12(6): 851.     CrossRef
  • Evaluation of murine OX40L-murine IgG1(MM1) fusion protein on immunogenicity against L. mexicana infection in BALB/c mice
    Hossein Rezvan, Selman A. Ali, Sahar Hamoon Navard, Robert Rees
    Comparative Immunology, Microbiology and Infectious Diseases.2023; 99: 102011.     CrossRef
  • Leishmania infection upregulates and engages host macrophage Argonaute 1, and system-wide proteomics reveals Argonaute 1-dependent host response
    Atieh Moradimotlagh, Stella Chen, Sara Koohbor, Kyung-Mee Moon, Leonard J. Foster, Neil Reiner, Devki Nandan
    Frontiers in Immunology.2023;[Epub]     CrossRef
  • Recent Patents in the Treatment and Prevention of Leishmaniasis
    Sukhbir K Shahid
    Pharmaceutical Patent Analyst.2023; 12(5): 237.     CrossRef
  • 6,050 View
  • 211 Download
  • 43 Web of Science
  • Crossref

Brief Communication

Identification of Free-Living Amoebas in Tap Water of Buildings with Storage Tanks in Korea
Da-In Lee, Sung Hee Park, Jong Hwan Baek, Jee Won Yoon, Soo Im Jin, Kwang Eon Han, Hak Sun Yu
Korean J Parasitol 2020;58(2):191-194.
Published online April 30, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.2.191
Free-living amoebas (FLAs) can cause severe disease in humans and animals when they become infected. However, there are no accurate survey reports on the prevalence of FLAs in Korea. In this study, we collected 163 tap water samples from buildings, apartments, and restrooms of highway service areas in 7 Korean provinces with high population density. All these buildings and facilities have water storage tanks in common. The survey was separated into categories of buildings, apartments, and highway service areas. Five hundred milliliters of tap water from each building was collected and filtered with 0.2 ?m pore filter paper. The filters were incubated in agar plates with heated E. coli at 25°C. After axenization, genomic DNA was collected from each FLA, and species classification was performed using partial 18S-rDNA PCR-sequencing analysis. We found that 12.9% of tap water from buildings with storage tanks in Korea was contaminated with FLAs. The highway service areas had the highest contamination rate at 33.3%. All of the FLAs, except one, were genetically similar to Vermamoeba vermiformis (Hartmannella vermiformis). The remaining FLA (KFA21) was very similar to Acanthamoeba lugdunensis (KA/E26). Although cases of human infection by V. vermiformis are very rare, we must pay attention to the fact that one-third of tap water supplies in highway service areas have been contaminated.

Citations

Citations to this article as recorded by  Crossref logo
  • A socioenvironmental approach to the nosogenic potential of freshwaters with presence of thermotolerant free-living amoebae in Costa Rica
    Johan Alvarado-Ocampo, Juan José Romero Zúñiga, Julián Castro, Frida Chaves Monge, Marco Ruiz Campos, Alexa Bustamante Cortés, Elizabeth Abrahams Sandí, Lissette Retana Moreira
    Frontiers in Public Health.2025;[Epub]     CrossRef
  • Presence and diversity of free-living amoebae and their potential application as water quality indicators
    Areum Choi, Ji Won Seong, Jeong Hyun Kim, Jun Young Lee, Hyun Jae Cho, Shin Ae Kang, Mi Kyung Park, Mi Jin Jeong, Seo Yeong Choi, Yu Jin Jeong, Hak Sun Yu
    Parasites, Hosts and Diseases.2024; 62(2): 180.     CrossRef
  • A Narrative Review of Acanthamoeba Isolates in Malaysia: Challenges in Infection Management and Natural Therapeutic Advancements
    Mohammad Wisman Abdul Hamid, Roslaini Bin Abd Majid, Victor Fiezal Knight Victor Ernest, Nik Noorul Shakira Mohamed Shakrin, Firdaus Mohamad Hamzah, Mainul Haque
    Cureus.2024;[Epub]     CrossRef
  • Proper Management for Rigid Gas Permeable Contact and Orthokeratology Lens
    Kyu Young Shim, Jong Hwa Jun
    Annals of Optometry and Contact Lens.2023; 22(1): 7.     CrossRef
  • Well water sources simultaneous contamination with Cryptosporidium and Acanthamoeba in East-Southeast Asia and Acanthamoeba spp. in biofilms in the Philippines
    Frederick R. Masangkay, Giovanni D. Milanez, Joseph D. Dionisio, Luzelle Anne G.-L. Ormita, Abel V. Alvarez, Panagiotis Karanis
    Science of The Total Environment.2022; 837: 155752.     CrossRef
  • Recognition of Cell Wall Mannosylated Components as a Conserved Feature for Fungal Entrance, Adaptation and Survival Within Trophozoites of Acanthamoeba castellanii and Murine Macrophages
    Marina da Silva Ferreira, Susana Ruiz Mendoza, Diego de Souza Gonçalves, Claudia Rodríguez-de la Noval, Leandro Honorato, Leonardo Nimrichter, Luís Felipe Costa Ramos, Fábio C. S. Nogueira, Gilberto B. Domont, José Mauro Peralta, Allan J. Guimarães
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • Free-living amoebae in an oil refinery wastewater treatment facility
    Saeid Andalib, Hanieh Mohammad Rahimi, Maryam Niyyati, Farzaneh Shalileh, Sara Nemati, Soheila Rouhani, Mohammad Reza Zali, Hamed Mirjalali, Panagiotis Karanis
    Science of The Total Environment.2022; 839: 156301.     CrossRef
  • Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation
    Da-In Lee, Sung Hee Park, Shin-Ae Kang, Do Hyun Kim, Sun Hyun Kim, So Yeon Song, Sang Eun Lee, Hak Sun Yu
    The Korean Journal of Parasitology.2022; 60(4): 229.     CrossRef
  • The increasing importance of Vermamoebavermiformis
    Ruqaiyyah Siddiqui, Zinb Makhlouf, Naveed Ahmed Khan
    Journal of Eukaryotic Microbiology.2021;[Epub]     CrossRef
  • 8,132 View
  • 155 Download
  • 7 Web of Science
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Case Report

The First Acanthamoeba keratitis Case of Non-Contact Lens Wearer with HIV Infection in Thailand
Napaporn Tananuvat, Natnaree Techajongjintana, Pradya Somboon, Anchalee Wannasan
Korean J Parasitol 2019;57(5):505-511.
Published online October 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.5.505
Acanthamoeba keratitis (AK) is a rare sight-threatening corneal infection, often reporting from contact lens wearers. An asymptomatic human immunodeficiency virus (HIV)-infected Thai male without history of contact lens use complained foreign body sensation at his left eye during motorbike riding. He had neither specific keratitis symptoms nor common drugs responding, which contributed to delayed diagnosis. By corneal re-scraping, Acanthamoeba-like cysts were detected by calcofluor white staining and agar culture. The etiological agent obtained from the culture was molecularly confirmed by Acanthamoeba spp.-specific PCR, followed by DNA sequencing. The results from BLAST and phylogenetic analysis based on the DNA sequences, revealed that the pathogen was Acanthamoeba T4, the major genotype most frequently reported from clinical isolates. The infection was successfully treated with polyhexamethylene biguanide resulting in corneal scar. This appears the first reported AK case from a non-contact lens wearer with HIV infection in Thailand. Although AK is sporadic in developing countries, a role of free-living Acanthamoeba as an opportunistic pathogen should not be neglected. The report would increase awareness of AK, especially in the case presenting unspecific keratitis symptoms without clinical response to empirical antimicrobial therapy.

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  • Diagnosis of Acanthamoeba Keratitis: Past, Present and Future
    Matthew Azzopardi, Yu Jeat Chong, Benjamin Ng, Alberto Recchioni, Abison Logeswaran, Darren S. J. Ting
    Diagnostics.2023; 13(16): 2655.     CrossRef
  • New Frontiers in Acanthamoeba Keratitis Diagnosis and Management
    Omar Shareef, Sana Shareef, Hajirah N. Saeed
    Biology.2023; 12(12): 1489.     CrossRef
  • Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, Iran
    Ehsan Javanmard, Maryam Niyyati, Ali Taghipour, Marziye Fatemi, Hamed Mirjalali, Panagiotis Karanis
    Journal of Water and Health.2022; 20(4): 620.     CrossRef
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    Hadi Khatoonaki, Kavous Solhjoo, Hassan Rezanezhad, Belal Armand, Amir Abdoli, Ali Taghipour
    Journal of Water and Health.2022; 20(7): 1126.     CrossRef
  • Detection of Acanthamoeba from Acanthamoeba Keratitis Mouse Model Using Acanthamoeba-Specific Antibodies
    Min-Jeong Kim, A-Jeong Ham, A-Young Park, Hae-Jin Sohn, Ho-Joon Shin, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
    Microorganisms.2022; 10(9): 1711.     CrossRef
  • RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
    Heekyoung Kang, Hae-Jin Sohn, A-Young Park, A-Jeong Ham, Jeong-Heon Lee, Young-Hwan Oh, Yong-Joon Chwae, Kyongmin Kim, Sun Park, Hongseok Yang, Suk-Yul Jung, Jong-Hyun Kim, Ho-Joon Shin
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  • Genotype distribution of Acanthamoeba in keratitis: a systematic review
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    Parasitology Research.2021; 120(9): 3051.     CrossRef
  • Explicating the presentations of Acanthamoeba keratitis with special concern in the COVID-19 pandemic ambient
    Chukwuma Sr Chrysanthus
    International Journal of Clinical Microbiology and Biochemical Technology.2021; 4(1): 010.     CrossRef
  • Effectiveness and Safety of Topical Chlorhexidine and Vitamin E TPGS in the Treatment of Acanthamoeba Keratitis: A Survey on 29 Cases
    Ciro Caruso, Daniela Eletto, Michele Rinaldi, Luigi Pacente, Salvatore Troisi, Francesco Semeraro, Roberto dell’Omo, Ciro Costagliola
    Journal of Clinical Medicine.2020; 9(11): 3775.     CrossRef
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  • 132 Download
  • 9 Web of Science
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Original Article

Mitochondrial DNA Sequence Variability of Spirometra Species in Asian Countries
Hyeong-Kyu Jeon, Keeseon S. Eom
Korean J Parasitol 2019;57(5):481-487.
Published online October 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.5.481
Mitochondrial DNA sequence variability of Spirometra erinaceieuropaei in GenBank was observed by reinvestigation of mitochondrial cox1 and cytb sequences. The DNA sequences were analyzed in this study, comprising complete DNA sequences of cox1 (n=239) and cytb (n=213) genes. The 10 complete mitochondrial DNA sequences of Spirometra species were compared with those of Korea, China and Japan. The sequences were analyzed for nucleotide composition, conserved sites, variable sites, singleton sites and parsimony-informative sites. Phylogenetic analyses was done using neighbor joining, maximum parsimony, Bayesian inference and maximum-likelihood on cox1 and cytb sequences of Spirometra species. These polymorphic sites identified 148 (cox1) and 83 (cytb) haplotypes within 239 and 213 isolates from 3 Asian countries. Phylogenetic tree topologies were presented high-level confidence values for the 2 major branches of 2 Spirometra species containing S. erinaceieuropaei and S. decipiens, and S. decipiens sub-clades including all sequences registered as S. erinaceieuropaei in cox1 and cytb genes. These results indicated that mitochondrial haplotypes of S. erinaceieuropaei and S. decipiens were found in the 3 Asian countries.

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    Mahboobeh Salimi, Meysam Sharifdini, Eshrat Beigom Kia
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    Fernando Fredes, Rubén Mercado, Ismael Pereira Salas, Hiromu Sugiyama, Hirotaka Kobayashi, Hiroshi Yamasaki
    Parasitology International.2022; 87: 102493.     CrossRef
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    Parasite Epidemiology and Control.2022; 17: e00245.     CrossRef
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    Fang Fang Xu, Wen Qing Chen, Wei Liu, Sha Sha Liu, Yi Xing Wang, Jing Chen, Jing Cui, Xi Zhang
    Parasites & Vectors.2022;[Epub]     CrossRef
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    Hiroshi Yamasaki, Oranuch Sanpool, Rutchanee Rodpai, Lakkhana Sadaow, Porntip Laummaunwai, Mesa Un, Tongjit Thanchomnang, Sakhone Laymanivong, Win Pa Pa Aung, Pewpan M. Intapan, Wanchai Maleewong
    Parasitology International.2021; 80: 102181.     CrossRef
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    The Korean Journal of Parasitology.2021; 59(2): 153.     CrossRef
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    Infection, Genetics and Evolution.2020; 85: 104466.     CrossRef
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    International Journal for Parasitology: Parasites and Wildlife.2020; 13: 142.     CrossRef
  • Ocular Sparganosis: The First Report of Spirometra ranarum in Thailand
    Wilai Saksirisampant, Chatanun Eamudomkarn, Hyeong-Kyu Jeon, Keeseon S. Eom, Buravej Assavapongpaiboon, Sunisa Sintuwong, Wasee Tulvatana
    The Korean Journal of Parasitology.2020; 58(5): 577.     CrossRef
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Brief Communications

Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam
Nguyen Thi Hong Chien, Thi Lan Nguyen, Khanh Linh Bui, Tho Van Nguyen, Thanh Hoa Le
Korean J Parasitol 2019;57(1):43-47.
Published online February 26, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.1.43
Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. A phylogenetic tree was inferred from 26 representative strains/species of Anaplasma spp. including 10 new sequences from Vietnam. Seven of our Vietnamese sequences fell into the clade of A. marginale and 3 into A. platys, with strong nodal support of 99 and 90%, respectively. Low genetic distances (0.2-0.4%) within each species supported the identification. Anaplasma platys is able to infect humans. Our discovery of this species in cattle and domestic dogs raises considerable concern about zoonotic transmission in Vietnam. Further systematic investigations are needed to gain data for Anaplasma spp. and members of Anaplasmataceae in animal hosts, vectors and humans across Vietnam.

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    Acta Tropica.2025; 268: 107700.     CrossRef
  • Isolation, Molecular Characterization and In Vitro Propagation of an Anaplasma platys-Like Bacterium in Tick Cells
    Erica Rodrigues de Matos, Priscilla Nunes dos Santos, Erich Peter Zweygarth, Talys Henrique Assumpção Jardim, Huarrisson Azevedo Santos, Matheus Dias Cordeiro, Bruna de Azevedo Baêta, Lesley Bell-Sakyi, Adivaldo Henrique da Fonseca, Claudia Bezerra da Sil
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    Frontiers in Tropical Diseases.2024;[Epub]     CrossRef
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    Madina Kadyrova, Alexandr Ostrovskii, Kassym Mukanov, Amirkhan Kassen, Elena Shevtsova, Maxat Berdikulov, Gilles Vergnaud, Alexandr Shevtsov
    Pathogens.2024; 13(10): 894.     CrossRef
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  • Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys in cattle in Nigeria
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  • Genetic diversity of Anaplasma marginale in calves with anaplasmosis on farms in Minas Gerais, Brazil
    Michele Bahia, Lucas Teixeira da Silva, Bruno Montijo da Silva, Matheus dias Cordeiro, Alexandro Guterres, Claudia Bezerra da Silva, Jenevaldo Barbosa da Silva, Adivaldo Henrique Fonseca
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    Barbara A. Qurollo, Brett A. Stillman, Melissa J. Beall, Paulette Foster, Barbara C. Hegarty, Edward B. Breitschwerdt, Ramaswamy Chandrashekar
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  • Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam
    Ly Na Huynh, Adama Zan Diarra, Quang Luan Pham, Nhiem Le-Viet, Jean-Michel Berenger, Van Hoang Ho, Xuan Quang Nguyen, Philippe Parola, Lynn Soong
    PLOS Neglected Tropical Diseases.2021; 15(9): e0009813.     CrossRef
  • The co-infection with Ehrlichia minasensis, Anaplasma marginale and Anaplasma platys is not associated with anemia in beef cattle in the Brazilian Pantanal
    Marcos Rogério André, Ana Cláudia Calchi, Heitor Miraglia Herrera, Diego Carlos de Souza Zanatto, Bruna de Carvalho Le Senéchal Horta, Júlia Banhareli Tasso, Inalda Angélica de Souza Ramos, Victória Valente Califre de Mello, Rosangela Zacarias Machado
    Veterinary Parasitology: Regional Studies and Reports.2020; 21: 100437.     CrossRef
  • Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand
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    BMC Veterinary Research.2020;[Epub]     CrossRef
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  • 132 Download
  • 17 Web of Science
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Comparison of Two PCR Assays for Trichomonas vaginalis
Chang-Suk Noh, Sang-Su Kim, Sung-Yul Park, Hong-Sang Moon, Yeonchul Hong, Jae-Sook Ryu
Korean J Parasitol 2019;57(1):27-31.
Published online February 26, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.1.27
PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

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  • Trikomoniyaz tanısında farklı laboratuvar yöntemlerinin kullanılması ve Trikomoniyaz hastalarında miRNA profilinin belirlenmesi
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    Phytopathology Research.2023;[Epub]     CrossRef
  • A microfluidic-chip-based system with loop-mediated isothermal amplification for rapid and parallel detection of Trichomonas vaginalis and human papillomavirus
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    The Analyst.2023; 148(19): 4820.     CrossRef
  • Development of Nested Polymerase Chain Reaction with Novel Specific Primers for Detection of Tritrichomonas muris Infection in Laboratory Mice
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    Animals.2023; 13(20): 3177.     CrossRef
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  • Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
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    BMC Infectious Diseases.2020;[Epub]     CrossRef
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Original Articles

Evaluation of Protective Immune Response Induced by a DNA Vaccine Encoding GRA8 against Acute Toxoplasmosis in a Murine Model
Jia-Qi Chu, Shuai Huang, Wei Ye, Xuan-Yan Fan, Rui Huang, Shi-Cai Ye, Cai-Yuan Yu, Wei-Yun Wu, Yu Zhou, Wei Zhou, Young-Ha Lee, Juan-Hua Quan
Korean J Parasitol 2018;56(4):325-334.
Published online August 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.4.325
Toxoplasma gondii is an apicomplexan zoonotic protozoan parasite that infects most species of warm-blooded animals, including humans. The heavy incidence and severe or lethal damage caused by T. gondii infection clearly indicate a need for the development of an effective vaccine. T. gondii GRA8 is a member of the dense granules protein family and is used as a marker of acute infection. In the present study, we evaluated the protective immunity induced by DNA vaccination based on a recombinant eukaryotic plasmid, pDsRed2-GRA8, against acute toxoplasmosis in mice. BALB/c mice were intramuscularly immunized with the pDsRed2-GRA8 plasmid and then challenged by infection with the highly virulent GFP-RH strain of T. gondii. The specific immune responses and protective efficacy against T. gondii of this vaccine were analyzed by measuring cytokine and serum antibody titers, splenocyte proliferation assays, and the survival times of mice after challenge. Our results showed that mice immunized with pDsRed2-GRA8 demonstrated specific humoral and cellular responses, induced higher IgG antibody titers with predominant IgG2a production; increased levels of IL-10, IL-12 (p70), IFN-γ, TNF-α, and splenocyte proliferation; and prolonged survival times compared to those of control mice. The present study showed that DNA immunization with pDsRed2-GRA8 induced humoral and cellular immune responses, and all immunized mice showed greater Th1-type immune responses and longer survival times than those of control mice. These results indicated that T. gondii GRA8 DNA immunization induces a partial protective effect against acute toxoplasmosis.

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  • Toxoplasma gondii vaccine candidates: a concise review
    Amirreza Javadi Mamaghani, Anwar Fathollahi, Zahra Arab-Mazar, Kobra kohansal, Matin Fathollahi, Adel Spotin, Homayoon Bashiri, Arezoo Bozorgomid
    Irish Journal of Medical Science (1971 -).2023; 192(1): 231.     CrossRef
  • Insight into the current Toxoplasma gondii DNA vaccine: a review article
    Xirui Zhang, Hao Yuan, Yasser S. Mahmmod, Zipeng Yang, Mengpo Zhao, Yining Song, Shengjun Luo, Xiu-Xiang Zhang, Zi-Guo Yuan
    Expert Review of Vaccines.2023; 22(1): 66.     CrossRef
  • Co-Immunization with DNA Vaccines Expressing SABP1 and SAG1 Proteins Effectively Enhanced Mice Resistance to Toxoplasma gondii Acute Infection
    Xiaoyu Sang, Xiang Li, Ran Chen, Ying Feng, Ting He, Xiaohan Zhang, Saeed El-Ashram, Ebtsam Al-Olayan, Na Yang
    Vaccines.2023; 11(7): 1190.     CrossRef
  • Evaluation of anti‐tick efficiency in rabbits induced by DNA vaccines encoding Haemaphysalis longicornis lipocalin homologue
    Xiang‐Yuan Fan, Xiao‐Can Xu, Ya‐Xue Wu, Xiao‐Ya Liu, Feng Yang, Yong‐Hong Hu
    Medical and Veterinary Entomology.2022; 36(4): 511.     CrossRef
  • Review of DNA Vaccine Approaches Against the Parasite Toxoplasma gondii
    Rosalie C. Warner, Ryan C. Chapman, Brianna N. Davis, Paul H. Davis
    Journal of Parasitology.2021;[Epub]     CrossRef
  • A systematic review on the role of GRA proteins of Toxoplasma gondii in host immunization
    Fatemeh Rezaei, Mahdi Sharif, Shahabeddin Sarvi, Seyed Hossein Hejazi, Sargis Aghayan, Abdol Sattar Pagheh, Samira Dodangeh, Ahmad Daryani
    Journal of Microbiological Methods.2019; 165: 105696.     CrossRef
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  • 172 Download
  • 8 Web of Science
  • Crossref
Immune Responses Induced by HSP60 DNA Vaccine against Toxoplasma gondii Infection in Kunming Mice
Zhong-Yuan Li, Jing Lu, Nian-Zhang Zhang, Jia Chen, Xing-Quan Zhu
Korean J Parasitol 2018;56(3):237-245.
Published online June 30, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.3.237
Toxoplasma gondii can infect all the vertebrates including human, and leads to serious toxoplasmosis and considerable veterinary problems. T. gondii heat shock protein 60 (HSP60) is associated with the activation of antigen presenting cells by inducing initial immune responses and releasing inflammatory cytokines. It might be a potential DNA vaccine candidate for this parasite. A pVAX-HSP60 DNA vaccine was constructed and immune responses was evaluated in Kunming mice in this study. Our data indicated that the innate and adaptive immune responses was elicited by successive immunizations with pVAX-HSP60 DNA, showing apparent increases of CD3e+CD4+ and CD3e+CD8a+ T cells in spleen tissues of the HSP60 DNA-immunized mice (24.70±1.23% and 10.90±0.89%, P<0.05) and higher levels of specific antibodies in sera. Furthermore, the survival period of the immunized mice (10.53±4.78 day) were significantly prolonged during the acute T. gondii infection. Decrease of brain cysts was significant in the experimental group during the chronic infection (P<0.01). Taken together, TgHSP60 DNA can be as a vaccine candidate to prevent the acute and chronic T. gondii infections.

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    Noé Lagunas-Cortés, Brenda Yomara García-Sánchez, Emmanuel Ríos-Castro, Marco Antonio Vega-López, Sirenia González-Pozos, Rubén Darío Diaz-Martín, Juan Manuel Carranza, Carlos J. Ramírez-Flores, Ricardo Mondragón-Flores
    Journal of Proteomics.2025; 317: 105454.     CrossRef
  • Live-attenuated PruΔgra72 strain of Toxoplasma gondii induces strong protective immunity against acute and chronic toxoplasmosis in mice
    Jing Li, Yu Kang, Ze-Xuan Wu, Shu-Feng Yang, Yu-Yang Tian, Xing-Quan Zhu, Xiao-Nan Zheng
    Parasites & Vectors.2024;[Epub]     CrossRef
  • Insight into the current Toxoplasma gondii DNA vaccine: a review article
    Xirui Zhang, Hao Yuan, Yasser S. Mahmmod, Zipeng Yang, Mengpo Zhao, Yining Song, Shengjun Luo, Xiu-Xiang Zhang, Zi-Guo Yuan
    Expert Review of Vaccines.2023; 22(1): 66.     CrossRef
  • Phylogenetic analysis of Babesia gibsoni isolates of south India using apical membrane antigen, 50 kDa surface antigen, and 70 kDa heat shock protein genes
    Chundayil Kalarickal Deepa, Anju Varghese, Christophe Angeline Felicia Bora, Karapparambu Gopalan Ajith Kumar, Lijo John, Muhasin Asaf, Sunanda Chulliparambil, Reghu Ravindran
    Experimental Parasitology.2023; 246: 108461.     CrossRef
  • Review of DNA Vaccine Approaches Against the Parasite Toxoplasma gondii
    Rosalie C. Warner, Ryan C. Chapman, Brianna N. Davis, Paul H. Davis
    Journal of Parasitology.2021;[Epub]     CrossRef
  • 8,191 View
  • 123 Download
  • 6 Web of Science
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An Alternative Method for Extracting Plasmodium DNA from EDTA Whole Blood for Malaria Diagnosis
Krongkaew Seesui, Kanokwan Imtawil, Phimphakon Chanetmahun, Porntip Laummaunwai, Thidarut Boonmars
Korean J Parasitol 2018;56(1):25-32.
Published online February 28, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.1.25
Molecular techniques have been introduced for malaria diagnosis because they offer greater sensitivity and specificity than microscopic examinations. Therefore, DNA isolation methods have been developed for easy preparation and cost effectiveness. The present study described a simple protocol for Plasmodium DNA isolation from EDTA-whole blood. This study demonstrated that after heating infected blood samples with Tris?EDTA buffer and proteinase K solution, without isolation and purification steps, the supernatant can be used as a DNA template for amplification by PCR. The sensitivity of the extracted DNA of Plasmodium falciparum and Plasmodium vivax was separately analyzed by both PCR and semi-nested PCR (Sn-PCR). The results revealed that for PCR the limit of detection was 40 parasites/μl for P. falciparum and 35.2 parasites/μl for P. vivax, whereas for Sn-PCR the limit of detection was 1.6 parasites/μl for P. falciparum and 1.4 parasites/μl for P. vivax. This new method was then verified by DNA extraction of whole blood from 11 asymptomatic Myanmar migrant workers and analyzed by Sn-PCR. The results revealed that DNA can be extracted from all samples, and there were 2 positive samples for Plasmodium (P. falciparum and P. vivax). Therefore, the protocol can be an alternative method for DNA extraction in laboratories with limited resources and a lack of trained technicians for malaria diagnosis. In addition, this protocol can be applied for subclinical cases, and this will be helpful for epidemiology and control.

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  • Evaluation of A Simple DNA Extraction Method and Its Combination with Loop-Mediated Isothermal Amplification Assays for Rapid Plasmodium knowlesi Diagnosis
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  • Liquid Biopsy for Promising Non-invasive Diagnostic Biomarkers in Parasitic Infections
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  • Comparison of six methods for Loa loa genomic DNA extraction
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    PLOS ONE.2022; 17(3): e0265582.     CrossRef
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    Redox Biology.2021; 48: 102177.     CrossRef
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DNA Vaccines Encoding Toxoplasma gondii Cathepsin C 1 Induce Protection against Toxoplasmosis in Mice
Yali Han, Aihua Zhou, Gang Lu, Guanghui Zhao, Wenchao Sha, Lin Wang, Jingjing Guo, Jian Zhou, Huaiyu Zhou, Hua Cong, Shenyi He
Korean J Parasitol 2017;55(5):505-512.
Published online October 31, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.5.505
Toxoplasma gondii cathepsin C proteases (TgCPC1, 2, and 3) are important for the growth and survival of T. gondii. In the present study, B-cell and T-cell epitopes of TgCPC1 were predicted using DNAstar and the Immune Epitope Database. A TgCPC1 DNA vaccine was constructed, and its ability to induce protective immune responses against toxoplasmosis in BALB/c mice was evaluated in the presence or absence of the adjuvant α-GalCer. As results, TgCPC1 DNA vaccine with or without adjuvant α-GalCer showed higher levels of IgG and IgG2a in the serum, as well as IL-2 and IFN-γ in the spleen compared to controls (PBS, pEGFP-C1, and α-Galcer). Upon challenge infection with tachyzoites of T. gondii (RH), pCPC1/α-Galcer immunized mice showed the longest survival among all the groups. Mice vaccinated with DNA vaccine without adjuvant (pCPC1) showed better protective immunity compared to other controls (PBS, pEGFP-C1, and α-Galcer). These results indicate that a DNA vaccine encoding TgCPC1 is a potential vaccine candidate against toxoplasmosis.

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  • Modest Protective Immune Responses Induced by a DNA Vaccine Expressing IMP1 of Toxoplasma gondii in BALB/c Mice
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    Parasite Immunology.2025;[Epub]     CrossRef
  • Insight into the current Toxoplasma gondii DNA vaccine: a review article
    Xirui Zhang, Hao Yuan, Yasser S. Mahmmod, Zipeng Yang, Mengpo Zhao, Yining Song, Shengjun Luo, Xiu-Xiang Zhang, Zi-Guo Yuan
    Expert Review of Vaccines.2023; 22(1): 66.     CrossRef
  • iNKT cell agonists as vaccine adjuvants to combat infectious diseases
    Ya-Qian Li, Cheng Yan, Rui Luo, Zheng Liu
    Carbohydrate Research.2022; 513: 108527.     CrossRef
  • Mining the Proteome of Toxoplasma Parasites Seeking Vaccine and Diagnostic Candidates
    Sajad Rashidi, Javier Sánchez-Montejo, Reza Mansouri, Mohammad Ali-Hassanzadeh, Amir Savardashtaki, Mohammad Saleh Bahreini, Mohammadreza Karimazar, Raúl Manzano-Román, Paul Nguewa
    Animals.2022; 12(9): 1098.     CrossRef
  • A Novel Combined DNA Vaccine Encoding Toxoplasma gondii SAG1 and ROP18 Provokes Protective Immunity Against a Lethal Challenge in Mice
    Lamei Wu, Huijian Yang, Jianglin Wang, Xiuwen Yu, Yanhong He, Shenxia Chen
    Acta Parasitologica.2021; 66(4): 1387.     CrossRef
  • Review of DNA Vaccine Approaches Against the Parasite Toxoplasma gondii
    Rosalie C. Warner, Ryan C. Chapman, Brianna N. Davis, Paul H. Davis
    Journal of Parasitology.2021;[Epub]     CrossRef
  • Characterization of metalloproteases and serine proteases of Toxoplasma gondii tachyzoites and their effect on epithelial cells
    Carlos J. Ramírez-Flores, Rosalba Cruz-Mirón, Rossana Arroyo, Mónica E. Mondragón-Castelán, Tais Nopal-Guerrero, Sirenia González-Pozos, Emmanuel Ríos-Castro, Ricardo Mondragón-Flores
    Parasitology Research.2019; 118(1): 289.     CrossRef
  • Antigenic Epitope Analysis and Efficacy Evaluation of GRA41 DNA Vaccine Against T. gondii Infection
    Jian Zhou, Chenxi Li, Yingquan Luo, Lin Wang
    Acta Parasitologica.2019; 64(3): 471.     CrossRef
  • Cysteine proteases in protozoan parasites
    Jair L. Siqueira-Neto, Anjan Debnath, Laura-Isobel McCall, Jean A. Bernatchez, Momar Ndao, Sharon L. Reed, Philip J. Rosenthal, Photini Sinnis
    PLOS Neglected Tropical Diseases.2018; 12(8): e0006512.     CrossRef
  • Immune Responses Induced by HSP60 DNA Vaccine against Toxoplasma gondii Infection in Kunming Mice
    Zhong-Yuan Li, Jing Lu, Nian-Zhang Zhang, Jia Chen, Xing-Quan Zhu
    The Korean Journal of Parasitology.2018; 56(3): 237.     CrossRef
  • Evaluation of Protective Immune Response Induced by a DNA Vaccine Encoding GRA8 against Acute Toxoplasmosis in a Murine Model
    Jia-Qi Chu, Shuai Huang, Wei Ye, Xuan-Yan Fan, Rui Huang, Shi-Cai Ye, Cai-Yuan Yu, Wei-Yun Wu, Yu Zhou, Wei Zhou, Young-Ha Lee, Juan-Hua Quan
    The Korean Journal of Parasitology.2018; 56(4): 325.     CrossRef
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Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran
Meysam Sharifdini, Zahra Heidari, Zahra Hesari, Sajad Vatandoost, Eshrat Beigom Kia
Korean J Parasitol 2017;55(3):279-285.
Published online June 30, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.3.279
The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0-1.8% and 0-0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.

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  • Helminth Infections in Cattle: A Study on Fecal Samples from the Ağrı Region
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    Van Veterinary Journal.2025; 36(2): 95.     CrossRef
  • Molecular identification of abomasal nematodes (Haemonchus spp. and Ostertagia ostertagi) in naturally infected calves
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    BULGARIAN JOURNAL OF VETERINARY MEDICINE.2024; 27(2): 286.     CrossRef
  • Trichostrongylosis: a zoonotic disease of small ruminants
    A.H. Bhat, H. Tak, I.M. Malik, B.A. Ganai, N. Zehbi
    Journal of Helminthology.2023;[Epub]     CrossRef
  • Prevalence and Morphometric Comparison of Trichostrongylus spp. among Sheep and Goats from Kashan Abattoir, Central Iran
    Mohsen Arbabi, Aimohammad Bakhshi, Hossein Hooshyar, Reza Ghasemikhah, Mahdi Delavari, Mojtaba Sehat
    Journal of Medical Microbiology and Infectious Diseases.2023; 11(1): 28.     CrossRef
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    Fatemeh Hajizadeh, Tahereh Mikaeili Galeh, Seyed Abdollah Hosseini, Seyyed Ali Shariatzadeh, Akram Hematizadeh, Javad Javidnia, Mitra Sadeghi, Mahdi Fakhar, Shirzad Gholami
    Parasite Epidemiology and Control.2023; 22: e00312.     CrossRef
  • Molecular identification of different Trichostrongylus species infecting sheep and goats from Dakahlia governorate, Egypt
    Rana Elseadawy, Ibrahim Abbas, Moustafa Al-Araby, Salah Abu-Elwafa
    Journal of Parasitic Diseases.2021; 45(1): 218.     CrossRef
  • Strongyloides stercoralis and other intestinal parasites in patients receiving immunosuppressive drugs in northern Iran: a closer look at risk factors
    Leila Mirzaei, Keyhan Ashrafi, Zahra Atrkar Roushan, Mohammad Reza Mahmoudi, Irandokht Shenavar Masooleh, Behnaz Rahmati, Farshid Saadat, Hamed Mirjalali, Meysam Sharifdini
    Epidemiology and Health.2021; 43: e2021009.     CrossRef
  • Comparison of Molecular and Parasitological Methods for Diagnosis of Human Trichostrongylosis
    Mehdi Pandi, Meysam Sharifdini, Keyhan Ashrafi, Zahra Atrkar Roushan, Behnaz Rahmati, Nayereh Hajipour
    Frontiers in Cellular and Infection Microbiology.2021;[Epub]     CrossRef
  • Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
    Hedayat Hosseinnezhad, Meysam Sharifdini, Keyhan Ashrafi, Zahra Atrkar Roushan, Hamed Mirjalali, Behnaz Rahmati
    BMC Veterinary Research.2021;[Epub]     CrossRef
  • Prevalence and risk factors of intestinal parasite infections in Mazandaran province, North of Iran
    Zeinab Gholipoor, Hooshang Khazan, Eznoallah Azargashb, Mohammad Reza Youssefi, Ali Rostami
    Clinical Epidemiology and Global Health.2020; 8(1): 17.     CrossRef
  • Zoonotic transmission of Teladorsagia circumcincta and Trichostrongylus species in Guilan province, northern Iran: molecular and morphological characterizations
    Keyhan Ashrafi, Meysam Sharifdini, Zahra Heidari, Behnaz Rahmati, Eshrat Beigom Kia
    BMC Infectious Diseases.2020;[Epub]     CrossRef
  • Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences
    Mohsen Arbabi, Hossein Hooshyar, Majid Lotfinia, Mohamad Ali Bakhshi
    Molecular and Biochemical Parasitology.2020; 236: 111260.     CrossRef
  • Phylogenetic analysis of Trichostrongylus vitrinus isolates from southwest Iran
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    Parasites & Vectors.2020;[Epub]     CrossRef
  • Clinical and haematological characteristics of human trichostrongyliasis
    L. Ghanbarzadeh, M. Saraei, E.B. Kia, F. Amini, M. Sharifdini
    Journal of Helminthology.2019; 93(2): 149.     CrossRef
  • Prevalence and phylogenetic analysis of gastrointestinal helminths (Nematoda: Trichostrongylidae) in ruminant livestock of northwest Iran
    Tahereh BARGHANDAN, Elham HAJİALİLO, Meysam SHARİFDİNİ, Amir JAVADİ
    Ankara Üniversitesi Veteriner Fakültesi Dergisi.2019; 67(1): 65.     CrossRef
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    Parasitology Research.2018; 117(10): 3183.     CrossRef
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    Meysam Sharifdini, Sedigheh Derakhshani, Safar Ali Alizadeh, Laleh Ghanbarzadeh, Hamed Mirjalali, Iraj Mobedi, Mehrzad Saraei
    Acta Tropica.2017; 176: 293.     CrossRef
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Brief Communication

Fasciola hepatica: Infection Status of Freshwater Snails Collected from Gangwon-do (Province), Korea
Jae-Hyung Lee, Juan-Hua Quan, In-Wook Choi, Gab-Man Park, Guang-Ho Cha, Hyun-Ju Kim, Jae-Min Yuk, Young-Ha Lee
Korean J Parasitol 2017;55(1):95-98.
Published online February 28, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.1.95
Fasciola hepatica is a trematode that causes zoonosis, mainly in cattle and sheep, and occasionally in humans. Few recent studies have determined the infection status of this fluke in Korea. In August 2015, we collected 402 samples of freshwater snails at Hoenggye-ri (upper stream) and Suha-ri (lower stream) of Song-cheon (stream) in Daegwalnyeong-myeon, Pyeongchang-gun in Gangwon-do (Province) near many large cattle or sheep farms. F. hepatica infection was determined using PCR on the nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 402 samples, F. hepatica 1TS-2 marker was detected in 6 freshwater snails; thus, the overall prevalence in freshwater snails was 1.5%. The prevalence varied between collection areas, ranging from 0.0% at Hoenggye-ri to 2.9% at Suha-ri. However, F. gigantica ITS-2 was not detected in the 6 F. hepatica-positive samples by PCR. The nucleotide sequences of the 6 F. hepatica ITS-2 PCR-positive samples were 99.4% identical to the F. hepatica ITS-2 sequences in GenBank, whereas they were 98.4% similar to F. gigantica ITS-2 sequences. These results indicated that the prevalence of F. hepatica in snail intermediate hosts was 1.5% in Gangwon-do, Korea; however the prevalence varied between collection areas. These results may help us to understand F. hepatica infection status in natural environments.

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  • Fasciola hepatica infection in Korean water deer (Hydropotes inermis argyropus)
    Na-Hyeon Kim, Min-Gyeong Seo, Bumseok Kim, Yu Jeong Jeon, In Jung Jung, Il-Hwa Hong
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  • Countering Zoonotic Diseases: Current Scenario and Advances in Diagnostics, Monitoring, Prophylaxis and Therapeutic Strategies
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    Archives of Medical Research.2024; 55(6): 103037.     CrossRef
  • The potential of snails as a source of food and feed
    Akash Gupta, Prabhat Khanal
    Journal of Agriculture and Food Research.2024; 18: 101330.     CrossRef
  • Molluscicidal and cercaricidal effects of Persicaria senegalensis on Radix natalensis snails and their echinostome-shed cercariae in South Africa
    MC Mathole, PH King
    African Journal of Aquatic Science.2023; 48(1): 71.     CrossRef
  • Epidemiology of Fasciola spp. in the intermediate host in China: A potential risk for fasciolosis transmission
    Ming Pan, Shao-Yuan Bai, Tian-Kai Ji, Yi-Min Fan, Dan-Dan Liu, Yi Yang, Jian-Ping Tao, Si-Yang Huang
    Acta Tropica.2022; 230: 106394.     CrossRef
  • Economic losses, morpho-molecular identification, and identity of Fasciola species recovered from Egypt
    Omima Ramadan Abdel‑Fatah, Waleed M. Arafa, Ahmed Anwar Wahba, Khaled Mohamed El‑Dakhly
    Journal of Parasitic Diseases.2022; 46(4): 1036.     CrossRef
  • Green vegetable juice as a potential source of human fascioliasis in Korea
    Sungim Choi, Sunghee Park, Sooji Hong, Hyejoo Shin, Bong-Kwang Jung, Min Jae Kim
    One Health.2022; 15: 100441.     CrossRef
  • Human and Animal Fascioliasis: Origins and Worldwide Evolving Scenario
    Santiago Mas-Coma, M. Adela Valero, M. Dolores Bargues
    Clinical Microbiology Reviews.2022;[Epub]     CrossRef
  • Living Liver Donor With Fasciola Hepatica: First Case Report in the Literature
    Sami Akbulut, Tevfik Tolga Sahin, Mehmet Kolu, Burak Isik, Yasar Bayindir, Sezai Yilmaz
    Experimental and Clinical Transplantation.2021; 19(3): 276.     CrossRef
  • Trematode cercarial fauna obtained from the field-collected freshwater snails Lymnaea natalensis in Egypt
    Amina M. Ibrahim, Amira Kamal Ahmed
    Bulletin of the National Research Centre.2019;[Epub]     CrossRef
  • Assessment of the molluscicidal impact of extracted chlorophyllin on some biochemical parameters in the nervous tissue and histological changes in Biomphalaria alexandrina and Lymnaea natalensis snails
    Amina M. Ibrahim, Fayez A. Bakry
    Invertebrate Neuroscience.2019;[Epub]     CrossRef
  • A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea
    Hyo-Seok Kim, Joo-Yeon Kong, Jong-Hyun Kim, Seong-Chan Yeon, Il-Hwa Hong
    The Korean Journal of Parasitology.2018; 56(4): 375.     CrossRef
  • Purification and biochemical characterization of a 22-kDa stable cysteine- like protease from the excretory-secretory product of the liver fluke Fasciola hepatica by using conventional techniques
    Ahmed Hemici, Roumaila Sabrina Benerbaiha, Dalila Bendjeddou
    Journal of Chromatography B.2017; 1068-1069: 268.     CrossRef
  • 10,004 View
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Original Articles

Platynosomum fastosum (Trematoda: Dicrocoeliidae) from Cats in Vietnam: Morphological Redescription and Molecular Phylogenetics
Hung Manh Nguyen, Hien Van Hoang, Loan Thi Ho
Korean J Parasitol 2017;55(1):39-45.
Published online February 28, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.1.39
The present study was performed to reveal the morphological characteristics and molecular phylogenetic position of Platynosomum fastosum Kossack, 1910. A total 167 specimens of P. fastosum were collected in 8 (4.9%) out of 163 sets of gall-bladders and bile ducts of cats. The number of worms was 1-105 per infected cat. This species was characterized by having a long and slender body, slightly larger ventral sucker than the oral sucker, indistinct prepharynx, small pharynx, short esophagus, bifurcation midway between 2 suckers, and ceca extending to the posterior end of the body. The length of the partial sequences of ITS1 and 5.8S rDNA of P. fastosum were 990 bp, GC-rich. AT/GC ratio was 0.9, there were 9 polymorphic sites, and intraspecific variations ranged from 0.1% to 0.9%. Phylogenetic analyses by neighbor-joining phylogram inferred from ITS1 rDNA sequences revealed that the genetic distance between P. fastosum specimens ranged from 0.3 to 1.5% while the smallest interspecific distance among dicrocoeliid species was 20.9 %. The redescription and genetic characters of P. fastosum are taxonomically important to recognize future different species of the genus Platynosomum showing high intraspecific and morphological variability.

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  • Occurrence and Risk Factors Associated with Platynosomum illiciens Infection in Cats with Elevated Liver Enzymes
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    Animals.2024; 14(7): 1065.     CrossRef
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    Zootaxa.2024; 5463(1): 1.     CrossRef
  • Survey on the prevalence of intestinal parasites in domestic cats (Felis catus Linnaeus, 1758) in central Nepal
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    Veterinary Medicine and Science.2023; 9(2): 559.     CrossRef
  • A systematic review and meta-analysis on the global status of Platynosomum sp. (Trematoda – Dicrocoelidae) infecting domestic cats (Felis catus)
    Welitânia Inácia Silva, Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela
    Veterinary Parasitology.2023; 322: 110031.     CrossRef
  • Antigenic components, identification, and characterization of whole worm extract of Platynosomum illiciens
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    Veterinary Parasitology.2022; 310: 109774.     CrossRef
  • Hepatic trematode Platynosomum sp. (Dicrocoeliidae) from a domestic cat in Colombo, Sri Lanka: Case report and molecular identification
    Dissanayaka Mudiyanselage Asha Erandhi Ranaraja, Thilini Anupama Nanayakkarawasam Mahakapuge, Dulari Samanthika Thilakarathne, Juliyabaduge Yushika Chamodi Dharmasuriya, Rajapakse Peramune Veddikkarage Jayanthe Rajapakse
    Veterinary Parasitology: Regional Studies and Reports.2022; 34: 100762.     CrossRef
  • A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis
    Babi Kyi Soe, Poom Adisakwattana, Onrapak Reamtong, Panat Anuracpreeda, Woraporn Sukhumavasi
    Veterinary World.2022; : 2029.     CrossRef
  • Platynosomum illiciens (Trematoda: Dicrocoeliidae) in a hybrid marmoset (Callithrix sp.) in the Municipality of Seropédica, RJ, Brazil – Case report
    Rayane Christine Pereira de Assis, Diefrey Ribeiro Campos, Debora Azevedo Borges, Barbara Rauta de Avelar, Julia Aline Santos de Mello Pereira, Carlos Alexandre Rey Matias, Carlos Wilson Gomes Lopes, Douglas McIntosh, Fabio Barbour Scott
    Revista Brasileira de Parasitologia Veterinária.2021;[Epub]     CrossRef
  • Clinical pathological findings of a cat with chronic cholangitis
    A. A. Ikhwan-Saufi, R. Ahmad-Rasul, H. X. Liew, M. Y. Lim, T. Adeline, R. Nuhanim, M. Daarulmuqaamah, A. Amlizawaty, M. Maizatul-Akmal, J. Johaimi, A. Rasedee, M. I. Mahiza, A. A. Azlina, H. Hazilawati
    Comparative Clinical Pathology.2020; 29(1): 17.     CrossRef
  • DNA sequences confirm low specificity to definitive host and wide distribution of the cat pathogen Platynosomum illiciens (= P. fastosum) (Trematoda: Dicrocoeliidae)
    Hudson A. Pinto, Eduardo A. Pulido-Murillo, Roberta R. Braga, Vitor L. T. Mati, Alan L. Melo, Vasyl V. Tkach
    Parasitology Research.2018; 117(6): 1975.     CrossRef
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Complete Mitochondrial Genome of Echinostoma hortense (Digenea: Echinostomatidae)
Ze-Xuan Liu, Yan Zhang, Yu-Ting Liu, Qiao-Cheng Chang, Xin Su, Xue Fu, Dong-Mei Yue, Yuan Gao, Chun-Ren Wang
Korean J Parasitol 2016;54(2):173-179.
Published online April 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.2.173
Echinostoma hortense (Digenea: Echinostomatidae) is one of the intestinal flukes with medical importance in humans. However, the mitochondrial (mt) genome of this fluke has not been known yet. The present study has determined the complete mt genome sequences of E. hortense and assessed the phylogenetic relationships with other digenean species for which the complete mt genome sequences are available in GenBank using concatenated amino acid sequences inferred from 12 protein-coding genes. The mt genome of E. hortense contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 non-coding region. The length of the mt genome of E. hortense was 14,994 bp, which was somewhat smaller than those of other trematode species. Phylogenetic analyses based on concatenated nucleotide sequence datasets for all 12 protein-coding genes using maximum parsimony (MP) method showed that E. hortense and Hypoderaeum conoideum gathered together, and they were closer to each other than to Fasciolidae and other echinostomatid trematodes. The availability of the complete mt genome sequences of E. hortense provides important genetic markers for diagnostics, population genetics, and evolutionary studies of digeneans.

Citations

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  • Genetic variation and population structure of Haemonchus contortus: an in-silico analysis
    W. Wei, Z. Lan, Xuewei Liu, Xinhui Zhang, X. Gu, R. Wang
    Journal of Helminthology.2025;[Epub]     CrossRef
  • The Nuclear Ribosomal Transcription Units of Two Echinostomes and Their Taxonomic Implications for the Family Echinostomatidae
    Yu Cao, Ye Li, Zhong-Yan Gao, Bo-Tao Jiang
    Biology.2025; 14(8): 1101.     CrossRef
  • The complete mitochondrial genome of Aspidogaster ijimai (Platyhelminthes: Trematoda: Aspidogastrea): gene content and phylogenetic inference
    D. A. Solodovnik, D. M. Atopkin, A. A. Semenchenko, M. Urabe, S. G. Sokolov
    Invertebrate Zoology.2025; 22(3): 411.     CrossRef
  • Complete mitochondrial genome and phylogenetic analysis of Dollfustrema vaneyi (Trematoda: Bucephalidae)
    Ye Hu, Tong Ye, Hong Zou, Gui-Tang Wang, Wen-Xiang Li, Dong Zhang
    BMC Genomics.2024;[Epub]     CrossRef
  • Characterization of the complete mitochondrial genome of Plagiorchis multiglandularis (Digenea, Plagiorchiidae): Comparison with the members of Xiphidiatan species and phylogenetic implications
    Janelle Laura J. Gacad, Natalia I. Yurlova, Natalia M. Ponomareva, Misako Urabe
    Parasitology Research.2023; 122(7): 1545.     CrossRef
  • A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences
    D. M. Atopkin, A. A. Semenchenko, D. A. Solodovnik, Y. I. Ivashko
    Journal of Helminthology.2023;[Epub]     CrossRef
  • The complete mitochondrial genome of Prosthogonimus cuneatus and Prosthogonimus pellucidus (Trematoda: Prosthogonimidae), their features and phylogenetic relationships in the superfamily Microphalloidea
    Xin-ru Guo, Ye Li, Yuan Gao, Yang-yuan Qiu, Zhen-hua Jin, Zhong-yan Gao, Xian-guang Zhang, Qi An, Qiao-cheng Chang, Jun-feng Gao, Chun-ren Wang
    Acta Tropica.2022; 232: 106469.     CrossRef
  • Characterization of complete mitochondrial genome and ribosomal operon forCarassotrema koreanumPark, 1938 (Digenea: Haploporidae) by means of next-generation sequencing data
    Y.I. Ivashko, A.A. Semenchenko, D.A. Solodovnik, D.M. Atopkin
    Journal of Helminthology.2022;[Epub]     CrossRef
  • Trematode diversity in freshwater snails from a stopover point for migratory waterfowls in Hokkaido, Japan: An assessment by molecular phylogenetic and population genetic analyses
    Minoru Nakao, Mizuki Sasaki
    Parasitology International.2021; 83: 102329.     CrossRef
  • First next-generation sequencing data for Haploporidae (Digenea: Haploporata): characterization of complete mitochondrial genome and ribosomal operon for Parasaccocoelium mugili Zhukov, 1971
    Dmitry M. Atopkin, Alexander A. Semenchenko, Daria A. Solodovnik, Yana I. Ivashko, Kirill A. Vinnikov
    Parasitology Research.2021; 120(6): 2037.     CrossRef
  • Characterization of the complete mitochondrial genome sequence of Tracheophilus cymbius (Digenea), the first representative from the family Cyclocoelidae
    Y. Li, X.X. Ma, Q.B. Lv, Y. Hu, H.Y. Qiu, Q.C. Chang, C.R. Wang
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Mitochondrial Genome Sequence of Echinostoma revolutum from Red-Crowned Crane (Grus japonensis)
    Rongkun Ran, Qi Zhao, Asmaa M. I. Abuzeid, Yue Huang, Yunqiu Liu, Yongxiang Sun, Long He, Xiu Li, Jumei Liu, Guoqing Li
    The Korean Journal of Parasitology.2020; 58(1): 73.     CrossRef
  • Characterization and comparative analysis of the complete mitochondrial genome of Azygia hwangtsiyui Tsin, 1933 (Digenea), the first for a member of the family Azygiidae
    Yuan-An Wu, Jin-Wei Gao, Xiao-Fei Cheng, Min Xie, Xi-Ping Yuan, Dong Liu, Rui Song
    ZooKeys.2020; 945: 1.     CrossRef
  • Characterization of the complete mitochondrial genome of Diplostomum baeri
    Toby Landeryou, Stephen M. Kett, Anne Ropiquet, Dirk Wildeboer, Scott P. Lawton
    Parasitology International.2020; 79: 102166.     CrossRef
  • A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity
    Michelle A. Gordy, Patrick C. Hanington
    Ecology and Evolution.2019; 9(6): 3153.     CrossRef
  • Characterization of the complete mitochondrial genome of Plagiorchis maculosus (Digenea, Plagiorchiidae), Representative of a taxonomically complex digenean family
    Suleman, Jun Ma, Mian Sayed Khan, Vasyl V. Tkach, Nehaz Muhammad, Dong Zhang, Xing-Quan Zhu
    Parasitology International.2019; 71: 99.     CrossRef
  • Characterization of the mitochondrial genome sequences of the liver fluke Amphimerus sp. (Trematoda: Opisthorchiidae) from Ecuador and phylogenetic implications
    Jun Ma, Jun-Jun He, Cheng-Yan Zhou, Miao-Miao Sun, William Cevallos, Hiromu Sugiyama, Xing-Quan Zhu, Manuel Calvopiña
    Acta Tropica.2019; 195: 90.     CrossRef
  • Characterization of the complete mitochondrial genome of Uvitellina sp., representative of the family Cyclocoelidae and phylogenetic implications
    Suleman, Mian Sayed Khan, Petr Heneberg, Cheng-Yan Zhou, Nehaz Muhammad, Xing-Quan Zhu, Jun Ma
    Parasitology Research.2019; 118(7): 2203.     CrossRef
  • The complete mitochondrial genome of Echinostoma miyagawai: Comparisons with closely related species and phylogenetic implications
    Ye Li, Yang-Yuan Qiu, Min-Hao Zeng, Pei-Wen Diao, Qiao-Cheng Chang, Yuan Gao, Yan Zhang, Chun-Ren Wang
    Infection, Genetics and Evolution.2019; 75: 103961.     CrossRef
  • Mitochondrial DNA Evidence Supports the Hypothesis that Triodontophorus Species Belong to Cyathostominae
    Yuan Gao, Yan Zhang, Xin Yang, Jian-Hua Qiu, Hong Duan, Wen-Wen Xu, Qiao-Cheng Chang, Chun-Ren Wang
    Frontiers in Microbiology.2017;[Epub]     CrossRef
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  • 130 Download
  • 22 Web of Science
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Brief Communication

Monitoring of Fasciola Species Contamination in Water Dropwort by COX1 Mitochondrial and ITS-2 rDNA Sequencing Analysis
In-Wook Choi, Hwang-Yong Kim, Juan-Hua Quan, Jae-Gee Ryu, Rubing Sun, Young-Ha Lee
Korean J Parasitol 2015;53(5):641-645.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.641
Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort is available. Here, we collected 500 samples of water dropwort in 3 areas in Korea during February and March 2015, and the water dropwort contamination of Fasciola species was monitored by DNA sequencing analysis of the Fasciola hepatica and Fasciola gigantica specific mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 500 samples assessed, the presence of F. hepatica cox1 and 1TS-2 markers were detected in 2 samples, and F. hepatica contamination was confirmed by sequencing analysis. The nucleotide sequences of cox1 PCR products from the 2 F. hepatica-contaminated samples were 96.5% identical to the F. hepatica cox1 sequences in GenBank, whereas F. gigantica cox1 sequences were 46.8% similar with the sequence detected from the cox1 positive samples. However, F. gigantica cox1 and ITS-2 markers were not detected by PCR in the 500 samples of water dropwort. Collectively, in this survey of the water dropwort contamination with Fasciola species, very low prevalence of F. hepatica contamination was detected in the samples.

Citations

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  • Isolation and molecular identification of liver fluke cercariae in freshwater snails of Chaharmahal and Bakhtiari province, Iran
    Bijan Hosseinpour Aghaei, Nadia Taiefi Nasrabadi, Yaser Pirali Kheirabadi, Seyed Shapoor Reza Shojaei
    Molluscan Research.2024; 44(1): 84.     CrossRef
  • Morphological and molecular identification of lymnaeid snail and trematodes cercariae in different water bodies in Perak, Malaysia
    Nazir Ahmad Tookhy, Nur Mahiza Md Isa, Rozaihan Mansor, Yasmin Abd Rahaman, Nur Indah Ahmad, Dung Thi Bui, Lokman Hakim Idris, Noor Hazfalinda Hamzah, Norhadila Zulkifli
    Parasitology Research.2023; 122(7): 1475.     CrossRef
  • Green vegetable juice as a potential source of human fascioliasis in Korea
    Sungim Choi, Sunghee Park, Sooji Hong, Hyejoo Shin, Bong-Kwang Jung, Min Jae Kim
    One Health.2022; 15: 100441.     CrossRef
  • Phylogenetic Characteristics of Fasciola hepatica Isolated from a Korean Patient
    Mi Jin Jeong, Jae Kyun Park, Hak Sun Yu
    The Korean Journal of Parasitology.2022; 60(5): 367.     CrossRef
  • A Descriptive Study of Human Fascioliasis in Qaemshahr, Mazandaran Province, Iran: Its Prevalence and Risk Factors
    Lotfollah Davoodi, Azadeh Mizani, Roya Najafi-Vosough, Saeed Hosseini Teshnizi, afsane amouei, Mousa Motavallihaghi, Hamideh Izadyar, Fateme Amuei, Sara Pourhaghighi, Seyed Reza Mirbadie, Eissa Soleymani
    Archives of Clinical Infectious Diseases.2022;[Epub]     CrossRef
  • A Review ofOenanthe javanica(Blume) DC. as Traditional Medicinal Plant and Its Therapeutic Potential
    Chuan-li Lu, Xiu-fen Li
    Evidence-Based Complementary and Alternative Medicine.2019; 2019: 1.     CrossRef
  • Human fascioliasis infection sources, their diversity, incidence factors, analytical methods and prevention measures
    S. Mas-Coma, M. D. Bargues, M. A. Valero
    Parasitology.2018; 145(13): 1665.     CrossRef
  • 9,901 View
  • 107 Download
  • 8 Web of Science
  • Crossref

Original Article

Effect of Farnesyltransferase Inhibitor R115777 on Mitochondria of Plasmodium falciparum
Young Ran Ha, Bae-Geun Hwang, Yeonchul Hong, Hye-Won Yang, Sang Joon Lee
Korean J Parasitol 2015;53(4):421-430.
Published online August 25, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.4.421
The parasite Plasmodium falciparum causes severe malaria and is the most dangerous to humans. However, it exhibits resistance to their drugs. Farnesyltransferase has been identified in pathogenic protozoa of the genera Plasmodium and the target of farnesyltransferase includes Ras family. Therefore, the inhibition of farnesyltransferase has been suggested as a new strategy for the treatment of malaria. However, the exact functional mechanism of this agent is still unknown. In addition, the effect of farnesyltransferase inhibitor (FTIs) on mitochondrial level of malaria parasites is not fully understood. In this study, therefore, the effect of a FTI R115777 on the function of mitochondria of P. falciparum was investigated experimentally. As a result, FTI R115777 was found to suppress the infection rate of malaria parasites under in vitro condition. It also reduces the copy number of mtDNA-encoded cytochrome c oxidase III. In addition, the mitochondrial membrane potential (ΔΨm) and the green fluorescence intensity of MitoTracker were decreased by FTI R115777. Chloroquine and atovaquone were measured by the mtDNA copy number as mitochondrial non-specific or specific inhibitor, respectively. Chloroquine did not affect the copy number of mtDNA-encoded cytochrome c oxidase III, while atovaquone induced to change the mtDNA copy number. These results suggest that FTI R115777 has strong influence on the mitochondrial function of P. falciparum. It may have therapeutic potential for malaria by targeting the mitochondria of parasites.

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    Raksha Kardam, Ashok Jangra, Dinesh Kumar, Vinod Kumar, Vikas Sharma
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  • Recent Advances in Diagnostics and Therapeutic Interventions for Drug-Resistant Malaria
    Kangkana Barman, Pranab Goswami
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  • Novel Therapeutics for Malaria
    Haitham Alaithan, Nirbhay Kumar, Mohammad Z. Islam, Angelike P. Liappis, Victor E. Nava
    Pharmaceutics.2023; 15(7): 1800.     CrossRef
  • Structure- and ligand-based drug design methods for the modeling of antimalarial agents: a review of updates from 2012 onwards
    Vivek K. Vyas, Sapna Bhati, Shivani Patel, Manjunath Ghate
    Journal of Biomolecular Structure and Dynamics.2022; 40(20): 10481.     CrossRef
  • Characterization of the promoter of the human farnesyltransferase beta subunit and the impact of the transcription factor OCT-1 on its expression
    Henning Verhasselt, Patrick Stelmach, Marie Domin, Dominik Jung, Anna Hagemann, Iris Manthey, Hagen S. Bachmann
    Genomics.2022; 114(2): 110314.     CrossRef
  • A comprehensive review on classifying fast-acting and slow-acting antimalarial agents based on time of action and target organelle of Plasmodium sp
    Monika Mariebernard, Abhinab Mohanty, Vinoth Rajendran
    Pathogens and Disease.2022;[Epub]     CrossRef
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    S. Daisy Precilla, Shreyas S. Kuduvalli, Anitha Thirugnanasambandhar Sivasubramanian
    Cell Biology International.2021; 45(1): 18.     CrossRef
  • The Repurposing of the Antimalaria Drug, Primaquine, as a Photosensitizer to Inactivate Cryptococcal Cells
    Uju L. Madu, Adepemi O. Ogundeji, Olufemi S. Folorunso, Jacobus Albertyn, Carolina H. Pohl, Olihile M. Sebolai
    Photochem.2021; 1(2): 275.     CrossRef
  • Live and Let Dye: Visualizing the Cellular Compartments of the Malaria Parasite Plasmodium falciparum
    Marleen Linzke, Sun Liu Rei Yan, Attila Tárnok, Henning Ulrich, Matthew R. Groves, Carsten Wrenger
    Cytometry Part A.2020; 97(7): 694.     CrossRef
  • Drug targets for resistant malaria: Historic to future perspectives
    Sahil Kumar, T.R. Bhardwaj, D.N. Prasad, Rajesh K. Singh
    Biomedicine & Pharmacotherapy.2018; 104: 8.     CrossRef
  • The Prenylated Proteome of Plasmodium falciparum Reveals Pathogen-specific Prenylation Activity and Drug Mechanism-of-action
    Jolyn E. Gisselberg, Lichao Zhang, Joshua E. Elias, Ellen Yeh
    Molecular & Cellular Proteomics.2017; 16(4): S54.     CrossRef
  • Exploring the putative self‐binding property of the human farnesyltransferase alpha‐subunit
    Anna Hagemann, Grit Müller, Iris Manthey, Hagen S. Bachmann
    FEBS Letters.2017; 591(21): 3637.     CrossRef
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  • 123 Download
  • 12 Web of Science
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Brief Communications

Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb
Chang Seok Oh, Min Seo, Jong Ha Hong, Jong-Yil Chai, Seung Whan Oh, Jun Bum Park, Dong Hoon Shin
Korean J Parasitol 2015;53(2):237-242.
Published online April 22, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.2.237
Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.

Citations

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  • Paleoparasitology research on ancient helminth eggs and larvae in the Republic of Korea
    Jong-Yil Chai, Min Seo, Dong Hoon Shin
    Parasites, Hosts and Diseases.2023; 61(4): 345.     CrossRef
  • Historic and Prehistoric Epidemics: An Overview of Sources Available for the Study of Ancient Pathogens
    Antoinette C. van der Kuyl
    Epidemiologia.2022; 3(4): 443.     CrossRef
  • Mummification in Korea and China: Mawangdui, Song, Ming and Joseon Dynasty Mummies
    Dong Hoon Shin, Raffaella Bianucci, Hisashi Fujita, Jong Ha Hong
    BioMed Research International.2018; 2018: 1.     CrossRef
  • Estimation of parasite infection prevalence of Joseon people by paleoparasitological data updates from the ancient feces of pre-modern Korean mummies
    MIN SEO, CHANG SEOK OH, JONG HA HONG, JONG-YIL CHAI, SOON CHUL CHA, YURI BANG, IN GUK CHA, YANG GUN WI, JUNG MIN PARK, DONG HOON SHIN
    Anthropological Science.2017; 125(1): 9.     CrossRef
  • A Case of Ectopic Paragonimiasis in a 17th Century Korean Mummy
    D. H. Shin, Y.-S. Kim, D. S. Yoo, M. J. Kim, C. S. Oh, J. H. Hong, E. Lee, J. Y. Chai, M. Seo
    Journal of Parasitology.2017; 103(4): 399.     CrossRef
  • Paleogenetic study on the 17th century Korean mummy with atherosclerotic cardiovascular disease
    Dong Hoon Shin, Chang Seok Oh, Jong Ha Hong, Yusu Kim, Soong Deok Lee, Eunju Lee, David Caramelli
    PLOS ONE.2017; 12(8): e0183098.     CrossRef
  • Ancient Ascaris DNA Sequences of Cytochrome B, Cytochrome C Oxidase Subunit 1, NADH Dehydrogenase Subunit 1, and Internal Transcribed Spacer 1 Genes from Korean Joseon Mummy Feces
    Jong Ha Hong, Chang Seok Oh, Min Seo, Jong-Yil Chai, Dong Hoon Shin
    Journal of Parasitology.2017; 103(6): 795.     CrossRef
  • 11,920 View
  • 129 Download
  • 7 Web of Science
  • Crossref
Molecular Characterization of Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae) from Goats in the Western Part of India by LSU of Nuclear Ribosomal DNA
Ashwani Kumar, Anshu Chaudhary, Chandni Verma, Hridaya Shanker Singh
Korean J Parasitol 2014;52(6):701-705.
Published online December 23, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.6.701

The rumen parasite, Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae), is a highly pathogenic trematode parasite of goat (Capra hircus). It sucks blood that causes acute disease like anemia, and severe economic losses occur due to morbidity and mortality of the ruminant infected by these worms. The study of these rumen paramphistomes, their infection, and public health importance remains unclear in India especially in the western part of state Uttar Pradesh (U.P.), Meerut, India, where the goat meat consumption is very high. This paper provides the molecular characterization of G. crumenifer recovered from the rumen of Capra hircus from Meerut, U.P., India by the partial sequence of 28S rDNA. Nucleotide sequence similarity searching on BLAST of 28S rDNA from parasites showed the highest identity with those of G. crumenifer from the same host Capra hircus. This is the first report of molecular identification of G. crumenifer from this part of India.

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  • A Universal Approach to Molecular Identification of Rumen Fluke Species Across Hosts, Continents, and Sample Types
    Gillian Mitchell, Ruth N. Zadoks, Philip J. Skuce
    Frontiers in Veterinary Science.2021;[Epub]     CrossRef
  • Rumen fluke, Fischoederius elongatus (Trematoda: Gastrothylacidae): Preliminary investigation of suitable conditions for egg hatching
    Sirapat Nak-on, Thapana Chontananarth
    Veterinary Parasitology.2020; 282: 109135.     CrossRef
  • Incidence of amphistome infection in sheep from Mhow, Madhya Pradesh
    D. K. Bansal, V. Agrawal, A. K. Jayraw, U. K. Garg, G. P Jatav, N. Jamra, Nidhi Singh
    Journal of Parasitic Diseases.2018; 42(1): 19.     CrossRef
  • First genetic evidence for the presence of the rumen fluke Paramphistomum epiclitum in Pakistan
    Qasim Ali, Imran Rashid, Muhammad Zubair Shabbir, Haroon Akbar, Kashif Shahzad, Kamran Ashraf, Neil Sargison, Umer Chaudhry
    Parasitology International.2018; 67(5): 533.     CrossRef
  • 11,098 View
  • 102 Download
  • 3 Web of Science
  • Crossref
Genetic Polymorphisms in VIR Genes among Indian Plasmodium vivax Populations
Purva Gupta, Veena Pande, Aparup Das, Vineeta Singh
Korean J Parasitol 2014;52(5):557-564.
Published online October 22, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.5.557

The vir genes are antigenic genes and are considered to be possible vaccine targets. Since India is highly endemic to Plasmodium vivax, we sequenced 5 different vir genes and investigated DNA sequence variations in 93 single-clonal P. vivax isolates. High variability was observed in all the 5 vir genes; the vir 1/9 gene was highly diverged across Indian populations. The patterns of genetic diversity do not follow geographical locations, as geographically distant populations were found to be genetically similar. The results in general present complex genetic diversity patterns in India, requiring further in-depth population genetic and functional studies.

Citations

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  • Genomic dynamics of clinical Plasmodium vivax: comparative genomic hybridization in severe malaria cases
    Sampreeti Tahbildar, Pon Arunachalam Boopathi, Sanjay Kumar Kochar, Dhanpat Kumar Kochar, Mohamed Aiyaz, Raja C. Mugasimangalam, Sudha N. Rao, Ashis Das
    Frontiers in Malaria.2025;[Epub]     CrossRef
  • Population genetic analysis of Plasmodium vivax vir genes in Pakistan
    Sylvatrie-Danne Dinzouna-Boutamba, Zin Moon, Sanghyun Lee, Sahib Gul Afridi, Hương Giang Lê, Yeonchul Hong, Byoung-Kuk Na, Youn-Kyoung Goo
    Parasites, Hosts and Diseases.2024; 62(3): 313.     CrossRef
  • Misidentification of Plasmodium Species by Cross-Reacting Primers and Cerebral Malaria Caused by Plasmodium vivax
    Anoopkrishna Rai, Deepak Sebastian Pinto, Praveen Rai, Srinivas Teerthanath, Indrani Karunasagar, Rama Adiga
    Journal of Health and Allied Sciences NU.2023; 13(04): 563.     CrossRef
  • Vivax Malaria and the Potential Role of the Subtelomeric Multigene vir Superfamily
    Youn-Kyoung Goo
    Microorganisms.2022; 10(6): 1083.     CrossRef
  • Humoral and cellular immune response to Plasmodium vivax VIR recombinant and synthetic antigens in individuals naturally exposed to P. vivax in the Republic of Korea
    Sanghyun Lee, Young-Ki Choi, Youn-Kyoung Goo
    Malaria Journal.2021;[Epub]     CrossRef
  • Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation
    Ui-han Son, Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Hae Soo Yun, Jung-Yeon Kim, So-Young Joo, Sookwan Jeong, Man Hee Rhee, Yeonchul Hong, Dong-Il Chung, Dongmi Kwak, Youn-Kyoung Goo
    The Korean Journal of Parasitology.2017; 55(2): 149.     CrossRef
  • Clinical manifestations and molecular mechanisms in the changing paradigm of vivax malaria in India
    Purva Gupta, Rajni Sharma, Jagdish Chandra, Virender Kumar, Ruchi Singh, Veena Pande, Vineeta Singh
    Infection, Genetics and Evolution.2016; 39: 317.     CrossRef
  • Acute kidney injury in malaria: An update
    Anand Chellappan, D.S. Bhadauria
    Clinical Queries: Nephrology.2016; 5(1): 26.     CrossRef
  • Plasmodium vivax msp-3α polymorphisms: analysis in the Indian subcontinent
    Anju Verma, Hema Joshi, Vineeta Singh, Anup Anvikar, Neena Valecha
    Malaria Journal.2016;[Epub]     CrossRef
  • Design, construction and validation of a Plasmodium vivax microarray for the transcriptome profiling of clinical isolates
    Pon Arunachalam Boopathi, Amit Kumar Subudhi, Sheetal Middha, Jyoti Acharya, Raja Chinnadurai Mugasimangalam, Sanjay Kumar Kochar, Dhanpat Kumar Kochar, Ashis Das
    Acta Tropica.2016; 164: 438.     CrossRef
  • 10,677 View
  • 102 Download
  • 9 Web of Science
  • Crossref

Case Report

Eosinophilic Pleuritis due to Sparganum: A Case Report
Youngmin Oh, Jeong-Tae Kim, Mi-Kyeong Kim, You-Jin Chang, Keeseon Eom, Jung-Gi Park, Ki-Man Lee, Kang-Hyeon Choe, Jin-Young An
Korean J Parasitol 2014;52(5):541-543.
Published online October 22, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.5.541

Sparganosis is a rare parasitic disease caused by migrating plerocercoid tapeworm larva of the genus Spirometra. Infection in humans is mainly caused by the ingestion of raw or inadequately cooked flesh of infected frogs, snakes, and chickens. Here, we report a rare case of a 45-year-old man who was admitted to our hospital with left lower chest pain. The chest radiograph and computed tomography (CT) scan revealed localized pleural effusion in the left lower lobe; further, peripheral blood eosinophilia and eosinophilic pleural effusion were present. Percutaneous catheter drainage was performed, which revealed long worm-shaped material that was identified as a sparganum by DNA sequencing. The patient showed clinical improvement after drainage of the sparganum. This study demonstrates the importance of considering parasitic diseases in the differential diagnosis of eosinophilic pleural effusion.

Citations

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    Zhen-zhen Pan, Miao-juan Zhu, Yu-qiong Rong, Jiong Yang
    BMC Infectious Diseases.2023;[Epub]     CrossRef
  • Epidemiology, Diagnosis, and Prevention of Sparganosis in Asia
    Wei Liu, Tengfang Gong, Shuyu Chen, Quan Liu, Haoying Zhou, Junlin He, Yong Wu, Fen Li, Yisong Liu
    Animals.2022; 12(12): 1578.     CrossRef
  • Incidence, aetiology and clinical features of eosinophilic pleural effusion: a retrospective study
    Minfang Li, Yunxiang Zeng, Yaqing Li, Dan Jia, Sheng Chen, Jinlin Wang
    BMC Pulmonary Medicine.2021;[Epub]     CrossRef
  • Recurrent eosinophilic pleuritis caused by sparganum infection
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    Medicine.2020; 99(22): e20226.     CrossRef
  • Retrospective study of pleural parasitic infestations: a practical diagnostic approach
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  • Clinical Features of Pulmonary Sparganosis
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    The American Journal of the Medical Sciences.2015; 350(6): 436.     CrossRef
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Original Article

DNA Extraction from Protozoan Oocysts/Cysts in Feces for Diagnostic PCR
Yousry Hawash
Korean J Parasitol 2014;52(3):263-271.
Published online June 26, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.3.263

PCR detection of intestinal protozoa is often restrained by a poor DNA recovery or by inhibitors present in feces. The need for an extraction protocol that can overcome these obstacles is therefore clear. QIAamp® DNA Stool Mini Kit (Qiagen) was evaluated for its ability to recover DNA from oocysts/cysts directly from feces. Twenty-five Giardia-positive, 15 Cryptosporidium-positive, 15 Entamoeba histolytica-positive, and 45 protozoa-free samples were processed as control by microscopy and immunoassay tests. DNA extracts were amplified using 3 sets of published primers. Following the manufacturer's protocol, the kit showed sensitivity and specificity of 100% towards Giardia and Entamoeba. However, for Cryptosporidium, the sensitivity and specificity were 60% (9/15) and 100%, respectively. A series of optimization experiments involving various steps of the kit's protocol were conducted using Cryptosporidium-positive samples. The best DNA recoveries were gained by raising the lysis temperature to the boiling point for 10 min and the incubation time of the InhibitEX tablet to 5 min. Also, using a pre-cooled ethanol for nucleic acid precipitation and small elution volume (50-100 ?l) were valuable. The sensitivity of the amended protocol to Cryptosporidium was raised to 100%. Cryptosporidium DNA was successfully amplified by either the first or the second primer set. When applied on parasite-free feces spiked with variable oocysts/cysts counts, ? 2 oocysts/cysts were theoretically enough for detection by PCR. To conclude, the Qiagen kit with the amended protocol was proved to be suitable for protozoan DNA extraction directly from feces and support PCR diagnosis.

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    Frontiers in Microbiology.2025;[Epub]     CrossRef
  • Evidence of Waterborne Parasites in Mussels for Human Consumption Harvested from a Recreational and Highly Productive Bay
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    Microorganisms.2025; 13(9): 1971.     CrossRef
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    Scientific Reports.2024;[Epub]     CrossRef
  • Evaluation of molecular-based methods for the detection and quantification of Cryptosporidium spp. in wastewater
    Oumaima Hachimi, Rebecca Falender, Gabriel Davis, Rispa Vranka Wafula, Melissa Sutton, June Bancroft, Paul Cieslak, Christine Kelly, Devrim Kaya, Tyler Radniecki
    Science of The Total Environment.2024; 947: 174219.     CrossRef
  • Nanoparticle Lysis of Cryptosporidium Oocysts
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    Methods and Protocols.2024; 7(5): 66.     CrossRef
  • Enhancing diagnostic accuracy: Direct immunofluorescence assay as the gold standard for detecting Giardia duodenalis and Cryptosporidium spp. in canine and feline fecal samples
    Juan P. Barrera, Guadalupe Miró, David Carmena, Carlos Foncubierta, Juliana Sarquis, Valentina Marino, Efrén Estévez-Sánchez, Begoña Bailo, Rocío Checa, Ana Montoya
    BMC Veterinary Research.2024;[Epub]     CrossRef
  • Cryptosporidium spp. in German wildlife: Detection, regional occurrence and diversity in wild boar, roe, red and fallow deer
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    Heliyon.2024; 10(21): e38548.     CrossRef
  • Plastic pollution and fungal, protozoan, and helminth pathogens – A neglected environmental and public health issue?
    Michael J. Ormsby, Ayorinde Akinbobola, Richard S. Quilliam
    Science of The Total Environment.2023; 882: 163093.     CrossRef
  • Current Applications of Digital PCR in Veterinary Parasitology: An Overview
    Constantina N. Tsokana, Isaia Symeonidou, Georgios Sioutas, Athanasios I. Gelasakis, Elias Papadopoulos
    Parasitologia.2023; 3(3): 269.     CrossRef
  • A rapid multiplex loop-mediated isothermal amplification (mLAMP) assay for detection of Entamoeba histolytica and Giardia duodenalis
    Abhishek Mewara, Sandhya Khunger, Chayan Sharma, Sivanantham Krishnamoorthi, Shreya Singh, Rakesh Yadav, Sumeeta Khurana, Rakesh Sehgal
    Letters in Applied Microbiology.2023;[Epub]     CrossRef
  • An Epidemiological Assessment of Cryptosporidium and Giardia spp. Infection in Pet Animals from Taiwan
    Chia-Hui Hsu, Chi Liang, Shi-Chien Chi, Kuan-Ju Lee, Chung-Hsi Chou, Chen-Si Lin, Wen-Yuan Yang
    Animals.2023; 13(21): 3373.     CrossRef
  • Development and evaluation of a molecular based protocol for detection and quantification of Cryptosporidium spp. in wastewater
    N.P. Mthethwa, I.D. Amoah, P. Reddy, F. Bux, S. Kumari
    Experimental Parasitology.2022; 234: 108216.     CrossRef
  • Pampas fox (Lycalopex gymnocercus) of the Argentine Pampas as intermediate host for Neospora caninum
    Nathalia Paula Scioscia, Yanina Paola Hecker, David Arranz-Solís, Julieta Pedrana, Facundo Nahuel Urtizbiria, Lucía María Campero, Leandro Olmos, María V. Scioli, Matías A. Dorsch, Franco Fiorani, Felipe Cheuquepan, Guillermo María Denegri, Gastón Moré, D
    Parasitology International.2022; 88: 102549.     CrossRef
  • Comparison Study of Four Extraction Methods Combined with PCR and LAMP for Feline Tritrichomonas foetus Detection in Fecal Samples
    Joanna Dąbrowska, Jacek Karamon, Maciej Kochanowski, Jacek Sroka, Jolanta Zdybel, Tomasz Cencek
    Pathogens.2022; 11(5): 604.     CrossRef
  • Detection and Molecular Identification of Cryptosporidium Species Among Children with Malignancies
    Heba Said Ibrahim, Amel Youssef Shehab, Amal Farahat Allam, Mostafa Aboelhoda Mohamed, Hoda Fahmy Farag, Mona Mohamed Tolba
    Acta Parasitologica.2021; 66(2): 377.     CrossRef
  • Prevalence and Phylogenetic Analysis of Microsporidium Enterocytozoon bieneusi in Diarrheal Patients
    Manman Zang, Jinjin Li, Chun Tang, Songtao Ding, Wei Huang, Qizhong Qin, Handeng Liu
    Pathogens.2021; 10(2): 128.     CrossRef
  • Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology
    Natalia Mannise, Andrés Cabrera, Hernán Juan, Mariana Cosse, Federico Giannitti, María E. Francia, Telma González, Andrés Iriarte, Franklin Riet~Correa, Carlos Robello, Susana González
    Molecular Biology Reports.2021; 48(6): 5013.     CrossRef
  • Optimization of routine microscopic and molecular detection of parasitic protozoa in SAF-fixed faecal samples in Sweden
    Jessica Ögren, Olaf Dienus, Andreas Matussek
    Infectious Diseases.2020; 52(2): 87.     CrossRef
  • Evaluation of Different Oocyst DNA Extraction Methods for Cryptosporidium spp. Research in Environmental Samples
    Neliane Cristina Moreira, Marlene Cabrine-Santos, Márcia Benedita de Oliveira-Silva
    Acta Parasitologica.2020; 65(4): 995.     CrossRef
  • Simultaneous Detection of Ascaris lumbricoides, Trichuris trichiura and Strongyloides stercoralis using Modified Stool DNA Extraction Method
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    Research Journal of Parasitology.2020; 16(1): 1.     CrossRef
  • MULTIPLEX POLYMERASE CHAIN REACTION ASSAY FOR THE DETECTION OF FIVE COMMON ENDOPARASITE INFESTATIONS IN LABORATORY RODENTS
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    Taiwan Veterinary Journal.2020; 46(04): 123.     CrossRef
  • First report and molecular characterization of Cryptosporidium spp. in humans and animals in Khartoum state, Sudan
    Kaltoum Yagoub Adam, A. A. Ismail, M. A. Masri, A. A. Gameel
    Veterinary World.2019; 12(1): 183.     CrossRef
  • Use of shotgun metagenomics for the identification of protozoa in the gut microbiota of healthy individuals from worldwide populations with various industrialization levels
    Ana Lokmer, Amandine Cian, Alain Froment, Nausicaa Gantois, Eric Viscogliosi, Magali Chabé, Laure Ségurel, Tiffany L. Weir
    PLOS ONE.2019; 14(2): e0211139.     CrossRef
  • Zoonotic potential of Giardia lamblia and control of giardiasis
    Fantinatti* Maria
    Insights in Veterinary Science.2019; 3(1): 001.     CrossRef
  • Practical Guidance for Clinical Microbiology Laboratories: Laboratory Diagnosis of Parasites from the Gastrointestinal Tract
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    Clinical Microbiology Reviews.2018;[Epub]     CrossRef
  • Assessment of the diagnostic performance of four methods for the detection of Giardia duodenalis in fecal samples from human, canine and feline carriers
    Flávia Fernandes de Mendonça Uchôa, Adriana Pittella Sudré, Sabrina Destri Emmerick Campos, Nádia Regina Pereira Almosny
    Journal of Microbiological Methods.2018; 145: 73.     CrossRef
  • Evaluation of Three Protocols of DNA Extraction for Detection of Giardia duodenalis in Human Fecal Specimens
    Fatemeh Asgarian, Mehdi Tavalla, Ali Teimoori, Nozhat Zebardast, Bahman Cheraghian
    Jundishapur Journal of Microbiology.2018;[Epub]     CrossRef
  • Evaluation of four commercial DNA extraction kits for the detection of Microsporidia and the importance of pretreatments in DNA isolation
    Ülfet Çetinkaya, Arzuv Charyyeva, Eda Sivcan, Esra Gürbüz
    Acta Parasitologica.2018; 63(2): 386.     CrossRef
  • Assessment of microscopic and molecular tools for the diagnosis and follow-up of cryptosporidiosis in patients at risk
    Y. Le Govic, K. Guyot, G. Certad, A. Deschildre, R. Novo, C. Mary, B. Sendid, E. Viscogliosi, L. Favennec, E. Dei-Cas, E. Fréalle, E. Dutoit
    European Journal of Clinical Microbiology & Infectious Diseases.2016; 35(1): 137.     CrossRef
  • Prevalence of intestinal parasite infections among patients in local public hospitals of Hail, Northwestern Saudi Arabia
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    Asian Pacific Journal of Tropical Medicine.2016; 9(1): 44.     CrossRef
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    Parasitology Research.2016; 115(9): 3551.     CrossRef
  • Evaluation of five commercial methods for the extraction and purification of DNA from human faecal samples for downstream molecular detection of the enteric protozoan parasites Cryptosporidium spp., Giardia duodenalis, and Entamoeba spp
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    Parasitology International.2016; 65(6): 635.     CrossRef
  • Clinical consequences of polymerase chain reaction‐based diagnosis of intestinal parasitic infections
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  • Prevalence of Cryptosporidium-Associated Diarrhea in a High Altitude-Community of Saudi Arabia Detected by Conventional and Molecular Methods
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Case Report

A Case of Diphyllobothrium nihonkaiense Infection as Confirmed by Mitochondrial COX1 Gene Sequence Analysis
Sang Hyun Park, Keeseon S. Eom, Min Sun Park, Oh Kyoung Kwon, Hyo Sun Kim, Jai Hoon Yoon
Korean J Parasitol 2013;51(4):471-473.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.471

Diphyllobothrium nihonkaiense has been reported in Korea as Diphyllobothrium latum because of their close morphologic resemblance. We have identified a human case of D. nihonkaiense infection using the mitochondrial cytochrome c oxidase subunit I (cox1) gene sequence analysis. On 18 February 2012, a patient who had consumed raw fish a month earlier visited our outpatient clinic with a long tapeworm parasite excreted in the feces. The body of the segmented worm was 2 m long and divided into the scolex (head) and proglottids. It was morphologically close to D. nihonkaiense and D. latum. The cox1 gene analysis showed 99.4% (340/342 bp) homology with D. nihonkaiense but only 91.8% (314/342 bp) homology with D. latum. The present study suggested that the Diphyllobothrium spp. infection in Korea should be analyzed with specific DNA sequence for an accurate species identification.

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    Gut Pathogens.2025;[Epub]     CrossRef
  • Repertory of eukaryotes (eukaryome) in the human gastrointestinal tract: taxonomy and detection methods
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    Parasite Immunology.2016; 38(1): 12.     CrossRef
  • Diphyllobothrium nihonkaiense: wide egg size variation in 32 molecularly confirmed adult specimens from Korea
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    Parasitology Research.2015; 114(6): 2129.     CrossRef
  • Three Cases of Diphyllobothrium nihonkaiense Infection in Korea
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    The Korean Journal of Parasitology.2014; 52(6): 677.     CrossRef
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    The Korean Journal of Parasitology.2014; 52(2): 197.     CrossRef
  • Parasitic Infections Based on 320 Clinical Samples Submitted to Hanyang University, Korea (2004-2011)
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    The Korean Journal of Parasitology.2014; 52(2): 215.     CrossRef
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Original Articles

Overexpression of Ubiquitin and Amino Acid Permease Genes in Association with Antimony Resistance in Leishmania tropica Field Isolates
Elham Kazemi-Rad, Mehdi Mohebali, Mohammad Bagher Khadem-Erfan, Homa Hajjaran, Ramtin Hadighi, Ali Khamesipour, Sassan Rezaie, Mojtaba Saffari, Reza Raoofian, Mansour Heidari
Korean J Parasitol 2013;51(4):413-419.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.413

The mainstay therapy against leishmaniasis is still pentavalent antimonial drugs; however, the rate of antimony resistance is increasing in endemic regions such as Iran. Understanding the molecular basis of resistance to antimonials could be helpful to improve treatment strategies. This study aimed to recognize genes involved in antimony resistance of Leishmania tropica field isolates. Sensitive and resistant L. tropica parasites were isolated from anthroponotic cutaneous leishmaniasis patients and drug susceptibility of parasites to meglumine antimoniate (Glucantime®) was confirmed using in vitro assay. Then, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) and real-time reverse transcriptase-PCR (RT-PCR) approaches were utilized on mRNAs from resistant and sensitive L. tropica isolates. We identified 2 known genes, ubiquitin implicated in protein degradation and amino acid permease (AAP3) involved in arginine uptake. Also, we identified 1 gene encoding hypothetical protein. Real-time RT-PCR revealed a significant upregulation of ubiquitin (2.54-fold), and AAP3 (2.86-fold) (P<0.05) in a resistant isolate compared to a sensitive one. Our results suggest that overexpression of ubiquitin and AAP3 could potentially implicated in natural antimony resistance.

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  • Leishmania spp. genetic factors associated with cutaneous leishmaniasis antimony pentavalent drug resistance: a systematic review
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    Memórias do Instituto Oswaldo Cruz.2024;[Epub]     CrossRef
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    Parasites & Vectors.2023;[Epub]     CrossRef
  • Quantitative proteomic analysis reveals differential modulation of crucial stage specific proteins during promastigote to amastigote differentiation in Leishmania donovani
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    Journal of Proteins and Proteomics.2022; 13(1): 17.     CrossRef
  • Leishmania infantum UBC1 in Metacyclic Promastigotes from Phlebotomus perniciosus, a Vaccine Candidate for Zoonotic Visceral Leishmaniasis
    Jaime Larraga, Pedro Alcolea, Ana Alonso, Luis Martins, Inmaculada Moreno, Mercedes Domínguez, Vicente Larraga
    Vaccines.2022; 10(2): 231.     CrossRef
  • Global distribution of treatment resistance gene markers for leishmaniasis
    Samira Salari, Mehdi Bamorovat, Iraj Sharifi, Pooya Ghasemi Nejad Almani
    Journal of Clinical Laboratory Analysis.2022;[Epub]     CrossRef
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    Frontiers in Pharmacology.2022;[Epub]     CrossRef
  • Metabolite Biomarkers of Leishmania Antimony Resistance
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    Cells.2021; 10(5): 1063.     CrossRef
  • Voriconazole resistance genes in Aspergillus flavus clinical isolates
    F. Zaini, E. Lotfali, A. Fattahi, E. Siddig, S. Farahyar, E. Kouhsari, M. Saffari
    Journal de Mycologie Médicale.2020; 30(2): 100953.     CrossRef
  • Comparative transcriptomic analysis of antimony resistant and susceptible Leishmania infantum lines
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    Parasites & Vectors.2020;[Epub]     CrossRef
  • An overview of leishmanization experience: A successful control measure and a tool to evaluate candidate vaccines
    Mehdi Mohebali, Abolhassan Nadim, Ali Khamesipour
    Acta Tropica.2019; 200: 105173.     CrossRef
  • Comparison of Cysteine Protease B Gene Expression between Clinical Isolates of Leishmania tropica, Leishmania major and Leishmania infantum
    Elham Kazemirad, Hossien Reisi Nafchi, Alireza Latifi, Reza Raoofian, Mehdi Mohebali, Homa Hajjaran
    Journal of Medical Microbiology and Infectious Diseases.2019; 7(3): 72.     CrossRef
  • Use of AFLP for the study of eukaryotic pathogens affecting humans
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    Acta Parasitologica.2016;[Epub]     CrossRef
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    International Journal of Dermatology.2016; 55(9): 1020.     CrossRef
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    The Korean Journal of Parasitology.2014; 52(2): 189.     CrossRef
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Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba
Hye-Won Yang, Yu-Ran Lee, Noboru Inoue, Bijay Kumar Jha, Dinzouna-Boutamba Sylvatrie Danne, Hong-Kyun Kim, Junhun Lee, Youn-Kyoung Goo, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong
Korean J Parasitol 2013;51(3):269-277.
Published online June 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.3.269

Amoebic keratitis (AK) caused by Acanthamoeba is one of the most serious corneal infections. AK is frequently misdiagnosed initially as viral, bacterial, or fungal keratitis, thus ensuring treatment delays. Accordingly, the early detection of Acanthamoeba would contribute significantly to disease management and selection of an appropriate anti-amoebic therapy. Recently, the loop-mediated isothermal amplification (LAMP) method has been applied to the clinical diagnosis of a range of infectious diseases. Here, we describe a rapid and efficient LAMP-based method targeting Acanthamoeba 18S rDNA gene for the detection of Acanthamoeba using clinical ocular specimens in the diagnosis of AK. Acanthamoeba LAMP assays detected 11 different strains including all AK-associated species. The copy number detection limit for a positive signal was 10 DNA copies of 18S rDNA per reaction. No cross-reactivity with the DNA of fungi or other protozoa was observed. The sensitivity of LAMP assay was higher than those of Nelson primer PCR and JDP primer PCR. In the present study, LAMP assay based on directly heat-treated samples was found to be as efficient at detecting Acanthamoeba as DNA extracted using a commercial kit, whereas PCR was only effective when commercial kit-extracted DNA was used. This study showed that the devised Acanthamoeba LAMP assay could be used to diagnose AK in a simple, sensitive, and specific manner.

Citations

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  • Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii
    Susanna Haapanen, Maarit S. Patrikainen, Seppo Parkkila
    Diagnostic Microbiology and Infectious Disease.2023; 107(2): 116014.     CrossRef
  • A simple and visible detection method for the rapid diagnosis of Ustilaginoidea virens in rice seeds by a loop‐mediated isothermal amplification assay
    Wei Wang, Hang Yin, Ning Huang, Cuijing Zhu, Yufei Wang, Xintong Qi, Lu Ma, Yunxin Fan, Yao Yu, Hongsheng Zhang, Yongmei Bao
    Journal of Phytopathology.2021; 169(6): 369.     CrossRef
  • Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample
    Tsui-Kang Hsu, Jung-Sheng Chen, Hsin-Chi Tsai, Chi-Wei Tao, Yu-Yin Yang, Ying-Chin Tseng, Yi-Jie Kuo, Dar-Der Ji, Jagat Rathod, Bing-Mu Hsu
    Scientific Reports.2021;[Epub]     CrossRef
  • Development of Visually Improved Loop Mediated Isothermal Amplification for the Diagnosis of Plasmodium vivax Malaria in a Tertiary Hospital in Chandigarh, North India
    Hargobinder Kaur, Rakesh Sehgal, Devendra Bansal, Ali A. Sultan, Ashish Bhalla, Sunit C. Singhi
    The American Journal of Tropical Medicine and Hygiene.2018; 98(5): 1374.     CrossRef
  • Detection of Acanthamoeba spp. in water samples collected from natural water reservoirs, sewages, and pharmaceutical factory drains using LAMP and PCR in China
    Anna Lass, Milena Guerrero, Xiuping Li, Gabriele Karanis, Liqing Ma, Panagiotis Karanis
    Science of The Total Environment.2017; 584-585: 489.     CrossRef
  • Water-borne protozoa parasites: The Latin American perspective
    Félix Manuel Rosado-García, Milena Guerrero-Flórez, Gabriele Karanis, María Del Carmen Hinojosa, Panagiotis Karanis
    International Journal of Hygiene and Environmental Health.2017; 220(5): 783.     CrossRef
  • Evaluation of Loop-mediated Isothermal Amplification Assay for Rapid Diagnosis of Acanthamoeba Keratitis
    Abhishek Mewara, Sumeeta Khurana, Shakila Yoonus, Kirti Megha, Parveen Tanwar, Amit Gupta, Rakesh Sehgal
    Indian Journal of Medical Microbiology.2017; 35(1): 90.     CrossRef
  • Acanthamoeba keratitis: improving the Scottish diagnostic service for the rapid molecular detection of Acanthamoeba species
    Claire Low Alexander, Michael Coyne, Brian Jones, Deepa Anijeet
    Journal of Medical Microbiology .2015; 64(7): 682.     CrossRef
  • Molecular diagnosis in clinical parasitology: When and why?
    Samson SY Wong, Kitty SC Fung, Sandy Chau, Rosana WS Poon, Sally CY Wong, Kwok-Yung Yuen
    Experimental Biology and Medicine.2014; 239(11): 1443.     CrossRef
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Protective and Anti-Pathology Effects of Sm Fructose-1,6-Bisphosphate Aldolase-Based DNA Vaccine against Schistosoma mansoni by Changing Route of Injection
Mohamed Saber, Tarek Diab, Olft Hammam, Amr Karim, Amina Medhat, Mamdouh Khela, Ehab El-Dabaa
Korean J Parasitol 2013;51(2):155-163.
Published online April 25, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.2.155

This study aimed to evaluate the efficacy of fructose-1,6-bis phosphate aldolase (SMALDO) DNA vaccination against Schistosoma mansoni infection using different routes of injection. The SMALDO has been cloned into the eukaryotic expression vector pcDNA3.1/V5-His TOPO-TA and was used in injecting Swiss albino mice intramuscularly (IM), subcutaneously (SC), or intraperitoneally (IP) (50 ?g/mouse). Mice vaccinated with non-recombinant pcDNA3.1 served as controls. Each group was immunized 4 times at weeks 0, 2, 4, and 6. Two weeks after the last booster dose, all mice groups were infected with 80 S. mansoni cercariae via tail immersion. At week 8 post-infection, animals were sacrificed for assessment of parasitological and histopathological parameters. High anti-SMALDO IgG antibody titers were detected in sera of all vaccinated groups (P<0.01) compared to the control group. Both the IP and SC vaccination routes resulted in a significant reduction in worm burden (46.2% and 28.9%, respectively, P<0.01). This was accompanied by a significant reduction in hepatic and intestinal egg counts (41.7% and 40.2%, respectively, P<0.01) in the IP group only. The number of dead eggs was significantly increased in both IP and IM groups (P<0.01). IP vaccination recorded the highest significant reduction in granuloma number and diameter (54.7% and 29.2%, respectively, P<0.01) and significant increase in dead miracidia (P<0.01). In conclusion, changing the injection route of SMALDO DNA vaccination significantly influenced the efficacy of vaccination. SMALDO DNA vaccination via IP route could be a promising protective and anti-pathology vaccine candidate against S. mansoni infection.

Citations

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  • Glycolytic Enzymes as Vaccines Against Schistosomiasis: Testing Schistosoma mansoni Phosphoglycerate Mutase in Mice
    David B. Pirovich, Akram A. Da'dara, Patrick J. Skelly
    Journal of Parasitology.2024;[Epub]     CrossRef
  • Multifunctional Fructose 1,6-Bisphosphate Aldolase as a Therapeutic Target
    David B. Pirovich, Akram A. Da’dara, Patrick J. Skelly
    Frontiers in Molecular Biosciences.2021;[Epub]     CrossRef
  • Characterization of Fructose-1,6-Bisphosphate Aldolase 1 of Echinococcus multilocularis
    Xuedong He, Jing Zhang, Yue Sun, Tianyan Lan, Xiaola Guo, Xiaoqiang Wang, Omnia M. Kandil, Mazhar Ayaz, Xuenong Luo, Houhui Song, Yadong Zheng
    Veterinary Sciences.2021; 9(1): 4.     CrossRef
  • Proteomic analysis of adult Schistosoma mekongi somatic and excretory-secretory proteins
    Onrapak Reamtong, Nattapon Simanon, Tipparat Thiangtrongjit, Yanin Limpanont, Phiraphol Chusongsang, Yupa Chusongsang, Songtham Anuntakarun, Sunchai Payungporn, Orawan Phuphisut, Poom Adisakwattana
    Acta Tropica.2020; 202: 105247.     CrossRef
  • Amelioration of type 1 diabetes by recombinant fructose-1,6-bisphosphate aldolase and cystatin derived from Schistosoma japonicum in a murine model
    Ke Yan, Bo Wang, Huabang Zhou, Qingli Luo, Jilong Shen, Yunxia Xu, Zhengrong Zhong
    Parasitology Research.2020; 119(1): 203.     CrossRef
  • Linking murine resistance to secondary cystic echinococcosis with antibody responses targeting Echinococcus granulosus tegumental antigens
    Sebastián Miles, Javier Magnone, Marek Cyrklaff, Paula Arbildi, Friedrich Frischknecht, Sylvia Dematteis, Gustavo Mourglia-Ettlin
    Immunobiology.2020; 225(3): 151916.     CrossRef
  • Interactome analysis of CD5 and CD6 ectodomains with tegumental antigens from the helminth parasite Echinococcus granulosus sensu lato
    Sebastián Miles, María Velasco-de-Andrés, Francisco Lozano, Gustavo Mourglia-Ettlin
    International Journal of Biological Macromolecules.2020; 164: 3718.     CrossRef
  • Protein extract from head-foot tissue of Oncomelania hupensis promotes the growth and development of mother sporocysts of Schistosoma japonicum via upregulation of parasite aldolase gene
    Ting Chai, Sijing Zhu, Huifen Dong, Zhenping Ming
    Parasitology Research.2019; 118(6): 1821.     CrossRef
  • Comparative study of excretory–secretory proteins released by Schistosoma mansoni-resistant, susceptible and naïve Biomphalaria glabrata
    Conor E. Fogarty, Min Zhao, Donald P. McManus, Mary G. Duke, Scott F. Cummins, Tianfang Wang
    Parasites & Vectors.2019;[Epub]     CrossRef
  • Why Do Intravascular Schistosomes Coat Themselves in Glycolytic Enzymes?
    David Pirovich, Akram A. Da'dara, Patrick J. Skelly
    BioEssays.2019;[Epub]     CrossRef
  • Schistosomiasis in Egypt: A never-ending story?
    Ahmad A. Othman, Rasha H. Soliman
    Acta Tropica.2015; 148: 179.     CrossRef
  • Cloning, expression, and partial characterization of FBPA from Schistosoma japonicum, a molecule on that the fluke may develop nutrition competition and immune evasion from human
    Qiping Hu, Huiqiong Xie, Shuyu Zhu, Dejun Liao, Tingzheng Zhan, Dengyu Liu
    Parasitology Research.2015; 114(9): 3459.     CrossRef
  • Evaluation of Echinostoma liei worm, metacercaria and redia antigens for schistosomiasis control
    G. Abdel-Monaem, A. Farid, I. Rabia, A. El-Amir
    Experimental Parasitology.2015; 157: 23.     CrossRef
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Efficacy of a DNA Vaccine Carrying Eimeria maxima Gam56 Antigen Gene against Coccidiosis in Chickens
Jinjun Xu, Yan Zhang, Jianping Tao
Korean J Parasitol 2013;51(2):147-154.
Published online April 25, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.2.147

To control coccidiosis without using prophylactic medications, a DNA vaccine targeting the gametophyte antigen Gam56 from Eimeria maxima in chickens was constructed, and the immunogenicity and protective effects were evaluated. The ORF of Gam56 gene was cloned into an eukaryotic expression vector pcDNA3.1(zeo)+. Expression of Gam56 protein in COS-7 cells transfected with recombinant plasmid pcDNA-Gam56 was confirmed by indirect immunofluorescence assay. The DNA vaccine was injected intramuscularly to yellow feathered broilers of 1-week old at 3 dosages (25, 50, and 100 ?g/chick). Injection was repeated once 1 week later. One week after the second injection, birds were challenged orally with 5×104 sporulated oocysts of E. maxima, then weighed and killed at day 8 post challenge. Blood samples were collected and examined for specific peripheral blood lymphocyte proliferation activity and serum antibody levels. Compared with control groups, the administration of pcDNA-Gam56 vaccine markedly increased the lymphocyte proliferation activity (P<0.05) at day 7 and 14 after the first immunization. The level of lymphocyte proliferation started to decrease on day 21 after the first immunization. A similar trend was seen in specific antibody levels. Among the 3 pcDNA-Gam56 immunized groups, the median dosage group displayed the highest lymphocyte proliferation and antibody levels (P<0.05). The median dosage group had the greatest relative body weight gain (89.7%), and the greatest oocyst shedding reduction (53.7%). These results indicate that median dosage of DNA vaccine had good immunogenicity and immune protection effects, and may be used in field applications for coccidiosis control.

Citations

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  • Alternatives to antibiotics against coccidiosis for poultry production: the relationship between immunity and coccidiosis management – a comprehensive review
    Mohamed T. El-Saadony, Heba M. Salem, Marwa M. Attia, Nahed Yehia, Ahmed H. Abdelkader, Sara Abdel Mawgod, Nesma Mohamed Kamel, Samar Sami Alkafaas, Muslimah N. Alsulami, Ahmed Ezzat Ahmed, Dina Mostafa Mohammed, Ahmed M. Saad, Walid F.A. Mosa, Shaaban S.
    Annals of Animal Science.2025;[Epub]     CrossRef
  • Insight into poultry coccidiosis: a dual focus on anticoccidial drugs and herbal plants for pharmacological and prevention strategies
    Pooja Bharti, Syed Tanveer, Zahoor Ahmad Wani, Shabir Ahmad Rather, Zahoor Ahmad Parray
    Journal of Parasitic Diseases.2025; 49(3): 565.     CrossRef
  • Development and immunological insights into recombinant/subunit vaccines against avian coccidiosis
    Shagufta Iqbal, Syed Tanveer, Idrees Mehraj Allaie, Yasmeena Jan, Shahana Tramboo, Nazima Maqbool
    Journal of Microbiological Methods.2025; 238: 107255.     CrossRef
  • Innovative prevention and control of coccidiosis: targeting sporogony for new control agent development
    Dan Zhao, Jingxia Suo, Lin Liang, Ruiying Liang, Rongqiong Zhou, Jiabo Ding, Xianyong Liu, Xun Suo, Sixin Zhang, Xinming Tang
    Poultry Science.2024; 103(12): 104246.     CrossRef
  • In vitro cultivation methods for coccidian parasite research
    Anna Sophia Feix, Teresa Cruz-Bustos, Bärbel Ruttkowski, Anja Joachim
    International Journal for Parasitology.2023; 53(9): 477.     CrossRef
  • Recombinant GMA56 and ROP17 of Eimeria magna conferred protection against infection by homologous species
    Jie Xiao, Hao Chen, Ruoyu Zheng, Jiayan Pu, Xiaobin Gu, Yue Xie, Ran He, Jing Xu, Bo Jing, Xuerong Peng, Guangyou Yang
    Frontiers in Immunology.2023;[Epub]     CrossRef
  • What Do We Know about Surface Proteins of Chicken Parasites Eimeria?
    Jesica Daiana Britez, Anabel Elisa Rodriguez, Lucía Di Ciaccio, Virginia Marugán-Hernandez, Mariela Luján Tomazic
    Life.2023; 13(6): 1295.     CrossRef
  • Cloning, expression and purification of Eimeria maxima gametocyte antigen-EmGam56 for control of poultry coccidiosis
    Vijayashanthi Ramalingam, Raman Muthusamy, Kasthuri Bohra, Azhahianambi Palavesam, Dhinakarraj Gopal
    Journal of Parasitic Diseases.2023; 47(4): 773.     CrossRef
  • Vaccination Against Poultry Parasites
    Ruediger Hauck, Kenneth S. Macklin
    Avian Diseases.2023;[Epub]     CrossRef
  • Coccidiosis: Recent Progress in Host Immunity and Alternatives to Antibiotic Strategies
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    Vaccines.2022; 10(2): 215.     CrossRef
  • A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens
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    Veterinary Research.2022;[Epub]     CrossRef
  • Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities
    Tean Zaheer, Rao Zahid Abbas, Muhammad Imran, Asghar Abbas, Ali Butt, Sarfraz Aslam, Jameel Ahmad
    Parasitology Research.2022; 121(10): 2749.     CrossRef
  • Research progress of the avian coccidiosis vaccine
    Haiming Cai, Nanshan Qi, Juan Li, Minna Lv, Xuhui Lin, Junjing Hu, Jianfei Zhang, Shenquan Liao, Mingfei Sun
    Veterinary Vaccine.2022; 1(1): 100002.     CrossRef
  • Protection of hatchlings against coccidiosis by maternal antibodies to four recombinant proteins of Eimeria tenella, Eimeria acervulina and Eimeria maxima
    Xu Yang, Xingju Song, Jing Liu, Qingzhong Chen, Tongwei An, Qun Liu
    Veterinary Parasitology.2022; 312: 109813.     CrossRef
  • Veteriner protozoolojide aşı uygulamalarına güncel yaklaşım
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    Journal of Advances in VetBio Science and Techniques.2021; 6(2): 179.     CrossRef
  • Brief History and Advancements of Vaccination Against Avian Coccidiosis: A Review

    Science Letters.2021; 9(2): 31.     CrossRef
  • Sexual Development in Non-Human Parasitic Apicomplexa: Just Biology or Targets for Control?
    Teresa Cruz-Bustos, Anna Sophia Feix, Bärbel Ruttkowski, Anja Joachim
    Animals.2021; 11(10): 2891.     CrossRef
  • Characterization of Cystoisospora suis sexual stages in vitro
    Anna Sophia Feix, Teresa Cruz-Bustos, Bärbel Ruttkowski, Anja Joachim
    Parasites & Vectors.2020;[Epub]     CrossRef
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    Shafiya Imtiaz Rafiqi, Rajat Garg, Hira Ram, K. K. Reena, Mayurkumar Asari, Priyanka Kumari, V. R. Kundave, Mithilesh Singh, P. S. Banerjee
    Parasitology Research.2019; 118(3): 945.     CrossRef
  • A review of Eimeria antigen identification for the development of novel anticoccidial vaccines
    J. Venkatas, M. A. Adeleke
    Parasitology Research.2019; 118(6): 1701.     CrossRef
  • Optimization of Immunization Procedure for Eimeria tenella DNA Vaccine pVAX1-pEtK2-IL-2 and Its Stability
    Bucai Zhang, Cheng Yuan, Xiaokai Song, Lixin Xu, Ruofeng Yan, Muhammad Ali A. Shah, Changming Guo, Shanyuan Zhu, Xiangrui Li
    Acta Parasitologica.2019; 64(4): 745.     CrossRef
  • Recent advances in delivery of veterinary DNA vaccines against avian pathogens
    Seyed Davoud Jazayeri, Chit Laa Poh
    Veterinary Research.2019;[Epub]     CrossRef
  • Passive immunization with Eimeria tenella gametocyte antigen 56 (EtGAM56) specific antibodies and active immunization trial with the epitope containing peptide
    Stefanie Wiedmer, Alaa Aldin Alnassan, Beate Volke, Ahmed Thabet, Arwid Daugschies, Matthias Lendner, Michael Kurth
    Veterinary Parasitology.2017; 247: 100.     CrossRef
  • Recombinant anticoccidial vaccines - a cup half full?
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    Infection, Genetics and Evolution.2017; 55: 358.     CrossRef
  • DNA vaccination of poultry: The current status in 2015
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    Vaccine.2016; 34(2): 202.     CrossRef
  • Development of immunization trials against Eimeria spp.
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    Trials in Vaccinology.2016; 5: 38.     CrossRef
  • Construction of Eimeria tenella multi-epitope DNA vaccines and their protective efficacies against experimental infection
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  • Immunization with recombinant 3-1E protein in AbISCO®-300 adjuvant induced protective immunity against Eimeria acervulina infection in chickens
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    Experimental Parasitology.2014; 141: 75.     CrossRef
  • Securing poultry production from the ever-present Eimeria challenge
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    Trends in Parasitology.2014; 30(1): 12.     CrossRef
  • Strategies for anti-coccidial prophylaxis
    DAVID M. WITCOMBE, NICHOLAS C. SMITH
    Parasitology.2014; 141(11): 1379.     CrossRef
  • 9,881 View
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Brief Communications

Human Infections with Liver and Minute Intestinal Flukes in Guangxi, China: Analysis by DNA Sequencing, Ultrasonography, and Immunoaffinity Chromatography
Hyeong-Kyu Jeon, Dongmin Lee, Hansol Park, Duk-Young Min, Han-Jong Rim, Hongman Zhang, Yichao Yang, Xueming Li, Keeseon S Eom
Korean J Parasitol 2012;50(4):391-394.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.391

The prevalence of liver and intestinal fluke infections was determined by surveying inhabitants of Hengxuan, Fusui, and Shanglin villages which were known to be endemic for liver flukes in Guangxi, China in May 2010. A total of 718 people were examined for helminth eggs by the Kato-Katz thick smear technique, ultrasonography, immunoaffinity chromatography, and DNA sequencing. The overall egg positive rate was found to be 59.6% (28.0-70.6%) that included mixed infections with liver and intestinal flukes. Cases showing higher than 20,000 eggs per gram of feces (EPG) were detected between 1.3% and 16.2%. Ultrasonographic findings exhibited overall 28.2% (72 of 255 cases) dilatation rate of the intrahepatic bile duct. Clonorchis sinensis infection was detected serologically in 88.3% (38 of 43 cases) among C. sinensis egg positive subjects by the immunoaffinity chromatography using a specific antigen for C. sinensis. For differential diagnosis of the liver and intestinal flukes, more precise PCR and nucleotide sequencing for copro-DNA were performed for 46 egg positive cases. Mixed infections with C. sinensis and Metagonimus yokogawai were detected in 8 of 46 egg positive cases, whereas 29 specimens were positive for Haplorchis taichui. Ultrasonographic findings and immunoaffinity chromatography results showed usefulness, even in a limited way, in figuring out of the liver fluke endemicity.

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    Parasitology Research.2024;[Epub]     CrossRef
  • Global prevalence of 4 neglected foodborne trematodes targeted for control by WHO: A scoping review to highlight the gaps
    Rachel Tidman, Kaushi S. T. Kanankege, Mathieu Bangert, Bernadette Abela-Ridder, Paul R. Torgerson
    PLOS Neglected Tropical Diseases.2023; 17(3): e0011073.     CrossRef
  • Incidence of fish-borne trematode infections and associated factors: results from a cohort study in highly endemic communities in northern Vietnam
    Thao Thi Bich Nguyen, Veronique Dermauw, Dung Thi Bui, Hafid Dahma, Dung Thuy Le, Hien Thi Thu Nguyen, Dung Trung Do, Pierre Dorny, Bertrand Losson, Olivier Vandenberg
    Parasitology Research.2023; 122(6): 1415.     CrossRef
  • PRESENCE OF METACERCARIAE OF Opisthorchis sp. BLANCHARD, 1895 (TREMATODA: OPISTHORCHIIDAE) IN NEW HOST Macrobrachium amazonicum (HELLER, 1862), FROM THE NORTHERN BRAZIL: AN EMERGING RISK TO HUMANS BY THE SHRIMP CONSUMPTION
    Déborah Elena Galvão Martins, Israel Hidenburgo Aniceto Cintra, Mayara Galvão Martins, Flavio de Almeida Alves-Júnior
    Environmental Smoke.2023; 6(2): 36.     CrossRef
  • Current status of Opisthorchis viverrini, minute intestinal fluke and Taenia spp. infections among inhabitants in the Kenethao district of northern Lao PDR
    Phasouk Senephansiri, Thaksaporn Thongseesuksai, Phaviny Sithay, Sakhone Laymanivong, Thidarut Boonmar, Porntip Laummaunwai
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2020; 114(5): 397.     CrossRef
  • Discovery of Carcinogenic Liver Fluke Metacercariae in Second Intermediate Hosts and Surveillance on Fish-Borne Trematode Metacercariae Infections in Mekong Region of Myanmar
    Ei Ei Phyo Myint, Amornpun Sereemaspun, Joacim Rocklöv, Choosak Nithikathkul
    International Journal of Environmental Research and Public Health.2020; 17(11): 4108.     CrossRef
  • Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
    Anh Kieu Thi Tran, Hoa Thuy Doan, Anh Ngoc Do, Van Thi Nguyen, Su Xuan Hoang, Huong Thu Thi Le, Hoa Thi Hoang, Nam Hoang Le, Quyen Bao Thi Le, Tran-Anh Le
    BioMed Research International.2019; 2019: 1.     CrossRef
  • Fishborne zoonotic heterophyid infections: An update
    Jong-Yil Chai, Bong-Kwang Jung
    Food and Waterborne Parasitology.2017; 8-9: 33.     CrossRef
  • Application of Ultrasonography in the Diagnosis of Infectious Diseases in Resource-Limited Settings
    Enrico Brunetti, Tom Heller, Joachim Richter, Daniel Kaminstein, Daniel Youkee, Maria Teresa Giordani, Samuel Goblirsch, Francesca Tamarozzi
    Current Infectious Disease Reports.2016;[Epub]     CrossRef
  • Point-of-Care Ultrasound Assessment of Tropical Infectious Diseases—A Review of Applications and Perspectives
    Sabine Bélard, Elizabeth Joekes, Francesca Tamarozzi, Tom Heller, Amaya L. Bustinduy, Walter Kuhn, Enrico Brunetti, Claudia Wallrauch, Martin P. Grobusch
    The American Journal of Tropical Medicine and Hygiene.2016; 94(1): 8.     CrossRef
  • Zoonotic helminths parasites in the digestive tract of feral dogs and cats in Guangxi, China
    Fang Fang, Jian Li, Tengfei Huang, Jacques Guillot, Weiyi Huang
    BMC Veterinary Research.2015;[Epub]     CrossRef
  • Correlation between clonorchiasis incidences and climatic factors in Guangzhou, China
    Tiegang Li, Zhicong Yang, Ming Wang
    Parasites & Vectors.2014;[Epub]     CrossRef
  • Complete Mitochondrial Genome of Haplorchis taichui and Comparative Analysis with Other Trematodes
    Dongmin Lee, Seongjun Choe, Hansol Park, Hyeong-Kyu Jeon, Jong-Yil Chai, Woon-Mok Sohn, Tai-Soon Yong, Duk-Young Min, Han-Jong Rim, Keeseon S. Eom
    The Korean Journal of Parasitology.2013; 51(6): 719.     CrossRef
  • Accuracy of the Kato-Katz method and formalin-ether concentration technique for the diagnosis of Clonorchis sinensis, and implication for assessing drug efficacy
    Men-Bao Qian, Peiling Yap, Yi-Chao Yang, Hai Liang, Zhi-Hua Jiang, Wei Li, Jürg Utzinger, Xiao-Nong Zhou, Jennifer Keiser
    Parasites & Vectors.2013;[Epub]     CrossRef
  • Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target
    Jing-ying Xiao, Ji-Yun Lee, Shinji Tokuhiro, Mitsuru Nagataki, Blanca R. Jarilla, Haruka Nomura, Tae Im Kim, Sung-Jong Hong, Takeshi Agatsuma, Banchob Sripa
    PLoS Neglected Tropical Diseases.2013; 7(11): e2548.     CrossRef
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Two Human Cases Infected by the Horsehair Worm, Parachordodes sp. (Nematomorpha: Chordodidae), in Japan
Minoru Yamada, Tatsuya Tegoshi, Niichiro Abe, Misako Urabe
Korean J Parasitol 2012;50(3):263-267.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.263

The present study was performed to describe 2 human cases infected by the horsehair worm, Parachordodes sp., in Japan. Two gordiid worms were collected in the vomit and excreta of an 80-year-old woman in November 2009 in Kyoto city, and in the mouth of 1-year-old boy in December 2009 in Nara city, Japan, respectively. Both worms were males having bifurcated posterior ends and male gonads in cross sectional specimens. They were identified as Parachordodes sp. (Nematomorpha: Chordodidae) based on the characteristic morphologies of cross sections and areoles in the cuticle. DNA analysis on 18S rRNA partial sequence arrangements was also carried out and both worms were assumed to be close to the genus Paragordionus based on tree analysis, and far from Gordius sp. which has already been reported in humans in Japan. DNA sequencing of the Parachordodes worm does not appear on the database; therefore, more information on the gene sequences of the genus Parachordodes from humans, animals, or intermediates is required.

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  • Oral cavity myiasis and mouth parasitic infestations: A systematic review
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    Journal of Stomatology Oral and Maxillofacial Surgery.2025; 126(5): 102425.     CrossRef
  • Invisible tenants: a case of parasitism in Akymnopellis chilensis (Gervais, 1847) (Chilopoda: Scolopendromorpha: Scolopendridae)
    E. Vega-Román
    Journal of Natural History.2025; 59(37-40): 2405.     CrossRef
  • Foodborne Diseases in the Edible Insect Industry in Europe—New Challenges and Old Problems
    Remigiusz Gałęcki, Tadeusz Bakuła, Janusz Gołaszewski
    Foods.2023; 12(4): 770.     CrossRef
  • Expression of Hairpin-Enriched Mitochondrial DNA in Two Hairworm Species (Nematomorpha)
    Olga V. Nikolaeva, Aleksandra M. Beregova, Boris D. Efeykin, Tatiana S. Miroliubova, Andrey Yu. Zhuravlev, Andrey Yu. Ivantsov, Kirill V. Mikhailov, Sergei E. Spiridonov, Vladimir V. Aleoshin
    International Journal of Molecular Sciences.2023; 24(14): 11411.     CrossRef
  • An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae
    Blaine A. Mathison, Sarah G. H. Sapp
    ZooKeys.2021; 1069: 1.     CrossRef
  • Laboratory Identification of Arthropod Ectoparasites
    Blaine A. Mathison, Bobbi S. Pritt
    Clinical Microbiology Reviews.2014; 27(1): 48.     CrossRef
  • 19,527 View
  • 130 Download
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Expression and Characterization of α-Methylacyl CoA Racemase from Anisakis simplex Larvae
Bong Jin Kim, Sun Mi Kim, Min Kyung Cho, Hak Sun Yu, Yong Seok Lee, Hee Jae Cha, Meesun Ock
Korean J Parasitol 2012;50(2):165-171.
Published online May 24, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.2.165

Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as α-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-40℃) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.

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  • The mitochondrial genome ofAncylostoma tubaeformefrom cats in China
    X.L. Shi, Y.Q. Fu, A.Y. Abdullahi, M.W. Wang, F. Yang, X.G. Yu, W.D. Pan, X.X. Yan, J.X. Hang, P. Zhang, G.Q. Li
    Journal of Helminthology.2018; 92(1): 22.     CrossRef
  • α-Methylacyl-CoA racemase (AMACR): Metabolic enzyme, drug metabolizer and cancer marker P504S
    Matthew D. Lloyd, Maksims Yevglevskis, Guat Ling Lee, Pauline J. Wood, Michael D. Threadgill, Timothy J. Woodman
    Progress in Lipid Research.2013; 52(2): 220.     CrossRef
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Original Article

Morphologic and Genetic Identification of Taenia Tapeworms in Tanzania and DNA Genotyping of Taenia solium
Keeseon S. Eom, Jong-Yil Chai, Tai-Soon Yong, Duk-Young Min, Han-Jong Rim, Charles Kihamia, Hyeong-Kyu Jeon
Korean J Parasitol 2011;49(4):399-403.
Published online December 16, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.4.399

Species identification of Taenia tapeworms was performed using morphologic observations and multiplex PCR and DNA sequencing of the mitochondrial cox1 gene. In 2008 and 2009, a total of 1,057 fecal samples were collected from residents of Kongwa district of Dodoma region, Tanzania, and examined microscopically for helminth eggs and proglottids. Of these, 4 Taenia egg positive cases were identified, and the eggs were subjected to DNA analysis. Several proglottids of Taenia solium were recovered from 1 of the 4 cases. This established that the species were T. solium (n=1) and T. saginata (n=3). One further T. solium specimen was found among 128 fecal samples collected from Mbulu district in Arusha, and this had an intact strobila with the scolex. Phylegenetic analysis of the mtDNA cox1 gene sequences of these 5 isolates showed that T. saginata was basal to the T. solium clade. The mitochondrial cox1 gene sequences of 3 of these Tanzanian isolates showed 99% similarity to T. saginata, and the other 2 isolates showed 100% similarity to T. solium. The present study has shown that Taenia tapeworms are endemic in Kongwa district of Tanzania, as well as in a previously identified Mbulu district. Both T. solium isolates were found to have an "African/Latin American" genotype (cox1).

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  • Current status of bovine cysticercosis and human taeniosis in areas surrounding Halaba Kulito Town, Central Ethiopia Regional State, Ethiopia
    Omer Idris, Jemere Bekele
    Veterinary Research Communications.2025;[Epub]     CrossRef
  • Proteomic and Phosphoproteomic Analysis of Adult Taenia saginata Revealed the Potential Importance of Cytoskeleton-Related Proteins
    Kang Liu, Kaijuan Wu, Xiaohua Liu, Jing Xie, Yixiao Wang, Chandara Ngim, Die Hu, Yangfan Xiao, Zheng Wang, Yisong Liu, Wei Liu, Liping Jiang
    Journal of Proteome Research.2025; 24(11): 5727.     CrossRef
  • Community Health-Education Intervention Trial against Human Taenia solium Taeniasis/Cysticercosis in Central and Southern Zones of Tanzania
    George Makingi, Bernard Ngowi, Ernatus Mkupasi, Christina Wilson, Andrea Sylvia Winkler, Jahashi Nzalawahe, Helena Ngowi
    Pathogens.2023; 12(7): 955.     CrossRef
  • Evidence for Transmission of Taenia solium Taeniasis/Cysticercosis in a Rural Area of Northern Rwanda
    Lucrecia Acosta Soto, Lucy Anne Parker, María José Irisarri-Gutiérrez, Javier Arturo Bustos, Yesenia Castillo, Erika Perez, Carla Muñoz-Antoli, José Guillermo Esteban, Héctor Hugo García, Fernando Jorge Bornay-Llinares
    Frontiers in Veterinary Science.2021;[Epub]     CrossRef
  • Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania
    Justine Daudi Maganira, Winifrida Kidima, Chacha John Mwita, Peter Halvarsson, Johan Höglund
    Infection Ecology & Epidemiology.2020;[Epub]     CrossRef
  • Taenia solium taeniosis and cysticercosis literature in Tanzania provides research evidence justification for control: A systematic scoping review
    Helena Aminiel Ngowi, Andrea Sylvia Winkler, Uffe Christian Braae, Robinson Hammerthon Mdegela, Ernatus Martin Mkupasi, Mwemezi Lutakyawa Kabululu, Faustin Peter Lekule, Maria Vang Johansen, Brecht Devleesschauwer
    PLOS ONE.2019; 14(6): e0217420.     CrossRef
  • Validation of droplet digital Polymerase Chain Reaction for the detection and absolute quantification of Taenia solium eggs in spiked soil samples
    Justine Daudi Maganira, Beda John Mwang'onde, Winifrida Kidima, Chacha John Mwita, Gamba Nkwengulila, Johan Höglund
    Acta Tropica.2019; 200: 105175.     CrossRef
  • Seroprevalence of circulating taeniid antigens in pigs and associated risk factors in Kongwa district, Tanzania
    Justine D. Maganira, Beda J. Mwang'onde, Winifrida Kidima, Chacha J. Mwita, Johan Höglund
    Parasite Epidemiology and Control.2019; 7: e00123.     CrossRef
  • Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in southern and eastern Africa
    Veronique Dermauw, Pierre Dorny, Uffe Christian Braae, Brecht Devleesschauwer, Lucy J. Robertson, Anastasios Saratsis, Lian F. Thomas
    Parasites & Vectors.2018;[Epub]     CrossRef
  • Prevalence and distribution of Taenia solium cysticercosis in naturally infected pigs in Punjab, India
    Satinder Pal Singh, Balbir Bagicha Singh, Deepali G. Kalambhe, Devendra Pathak, Rabinder Singh Aulakh, Navneet K. Dhand, Hector H. Garcia
    PLOS Neglected Tropical Diseases.2018; 12(11): e0006960.     CrossRef
  • Recent advances and perspectives in molecular epidemiology of Taenia solium cysticercosis
    Akira Ito, Tetsuya Yanagida, Minoru Nakao
    Infection, Genetics and Evolution.2016; 40: 357.     CrossRef
  • Monitoring the outcomes of interventions against Taenia solium: options and suggestions
    M. W. Lightowlers, H. H. Garcia, C. G. Gauci, M. Donadeu, B. Abela‐Ridder
    Parasite Immunology.2016; 38(3): 158.     CrossRef
  • Taenia solium taeniosis/cysticercosis and the co-distribution with schistosomiasis in Africa
    Uffe Christian Braae, Christopher F. L. Saarnak, Samson Mukaratirwa, Brecht Devleesschauwer, Pascal Magnussen, Maria Vang Johansen
    Parasites & Vectors.2015;[Epub]     CrossRef
  • Assessment of sanitary conditions of unregistered pig slaughter slabs and post mortem examination of pigs for Taenia solium metacestodes in Kaduna metropolis, Nigeria
    Agnes U Edia-Asuke, Helen I Inabo, Veronica J Umoh, Clement MZ Whong, Sunday Asuke, Richard E Edeh
    Infectious Diseases of Poverty.2014;[Epub]     CrossRef
  • Evolution, molecular epidemiology and perspectives on the research of taeniid parasites with special emphasis on Taenia solium
    Raúl J. Bobes, Gladis Fragoso, Agnès Fleury, Martín García-Varela, Edda Sciutto, Carlos Larralde, Juan P. Laclette
    Infection, Genetics and Evolution.2014; 23: 150.     CrossRef
  • Molecular diagnosis in clinical parasitology: When and why?
    Samson SY Wong, Kitty SC Fung, Sandy Chau, Rosana WS Poon, Sally CY Wong, Kwok-Yung Yuen
    Experimental Biology and Medicine.2014; 239(11): 1443.     CrossRef
  • Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1
    Wen Hui Li, Wan Zhong Jia, Zi Gang Qu, Zhi Zhou Xie, Jian Xun Luo, Hong Yin, Xiao Lin Sun, Radu Blaga, Bao Quan Fu
    The Korean Journal of Parasitology.2013; 51(2): 197.     CrossRef
  • Taenia solium taeniosis/cysticercosis in Africa: Risk factors, epidemiology and prospects for control using vaccination
    Emmanuel Assana, Marshall W. Lightowlers, André P. Zoli, Stanny Geerts
    Veterinary Parasitology.2013; 195(1-2): 14.     CrossRef
  • Molecular Approaches to Taenia asiatica
    Hyeong-Kyu Jeon, Keeseon S. Eom
    The Korean Journal of Parasitology.2013; 51(1): 1.     CrossRef
  • 11,104 View
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Brief Communications

PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
Joung-Ho Moon, Shin-Hyeong Cho, Jae-Ran Yu, Won-Ja Lee, Hyeng-Il Cheun
Korean J Parasitol 2011;49(3):281-284.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.281

Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples.

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    Lynne S. Garcia, Michael Arrowood, Evelyne Kokoskin, Graeme P. Paltridge, Dylan R. Pillai, Gary W. Procop, Norbert Ryan, Robyn Y. Shimizu, Govinda Visvesvara
    Clinical Microbiology Reviews.2018;[Epub]     CrossRef
  • Evaluation of recombinant multi-epitope proteins for diagnosis of goat schistosomiasis by enzyme-linked immunosorbent assay
    Chao Lv, Yang Hong, Zhiqiang Fu, Ke Lu, Xiaodan Cao, Tao Wang, Chuangang Zhu, Hao Li, Rui Xu, Bingguang Jia, Qian Han, Xuefeng Dou, Yuanxi Shen, Zuhang Zhang, Jinli Zai, Jintao Feng, Jiaojiao Lin
    Parasites & Vectors.2016;[Epub]     CrossRef
  • Frequency of amoebiasis and other intestinal parasitoses in a settlement in Ilhéus City, State of Bahia, Brazil
    Helena Lúcia Carneiro Santos, Luci Ana Fernandes Martins, Regina Helena Saramago Peralta, José Mauro Peralta, Heloisa Werneck de Macedo
    Revista da Sociedade Brasileira de Medicina Tropical.2014; 47(1): 101.     CrossRef
  • 9,687 View
  • 101 Download
  • Crossref
Molecular Identification of Taenia Tapeworms by Cox1 Gene in Koh Kong, Cambodia
Hyeong-Kyu Jeon, Tai-Soon Yong, Woon-Mok Sohn, Jong-Yil Chai, Sung-Jong Hong, Eun-Taek Han, Hoo-Gn Jeong, Tep Chhakda, Muth Sinuon, Duong Socheat, Keeseon S. Eom
Korean J Parasitol 2011;49(2):195-197.
Published online June 14, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.2.195

We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.

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  • Taeniasis impacts human gut microbiome composition and function
    Wenjie Mu, Pingping Ma, Yugui Wang, Yaqi Li, Yingying Ding, Yang Zou, Lixia Pu, Qi Yan, Haoyue Kong, Xiaola Guo, Aijiang Guo, Hailong Li, Shuai Wang
    The ISME Journal.2024;[Epub]     CrossRef
  • Taenia

    CABI Compendium.2022;[Epub]     CrossRef
  • Prevalence of meat-transmitted Taenia and Trichinella parasites in the Far East countries
    Yi Liu, Zijian Dong, Jianda Pang, Mingyuan Liu, Xuemin Jin
    Parasitology Research.2021; 120(12): 4145.     CrossRef
  • Occurrence of a Hybrid Between Taenia saginata and Taenia asiatica Tapeworms in Cambodia
    Taehee Chang, Bong-Kwang Jung, Sooji Hong, Hyejoo Shin, Seungwan Ryoo, Jeonggyu Lee, Keon Hoon Lee, Hansol Park, Keeseon S. Eom, Virak Khieu, Rekol Huy, Woon-Mok Sohn, Jong-Yil Chai
    The Korean Journal of Parasitology.2021; 59(2): 179.     CrossRef
  • Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development
    Hanif Ullah, Abdul Qadeer, Muhammad Rashid, Muhammad Imran Rashid, Guofeng Cheng
    Parasitology.2020; 147(4): 383.     CrossRef
  • Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia
    Ramon M. Eichenberger, Lian F. Thomas, Sarah Gabriël, Branco Bobić, Brecht Devleesschauwer, Lucy J. Robertson, Anastasios Saratsis, Paul R. Torgerson, Uffe C. Braae, Veronique Dermauw, Pierre Dorny
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Cysticercosis/taeniasis endemicity in Southeast Asia: Current status and control measures
    Hai-Wei Wu, Akira Ito, Lin Ai, Xiao-Nong Zhou, Luz P. Acosta, Arve Lee Willingham III
    Acta Tropica.2017; 165: 121.     CrossRef
  • Needs of exploring the burden of recent onset seizures due to neurocysticercosis and challenges in southeast Asia focusing on scenario in Malaysia
    Priyadarshi S. Sahu, Yvonne A.L. Lim, Rohela Mahmud, Sushela D. Somanath, Chong T. Tan, C.P. Ramachandran
    Asian Pacific Journal of Tropical Medicine.2017; 10(4): 332.     CrossRef
  • Advances in molecular diagnosis of parasitic enteropathogens
    Shane Byrne, Jennifer M.B. Robson
    Pathology.2015; 47(3): 234.     CrossRef
  • Intestinal Parasite Infections in Symptomatic Children Attending Hospital in Siem Reap, Cambodia
    Catrin E. Moore, Phot Nget, Mao Saroeun, Suy Kuong, Seng Chanthou, Varun Kumar, Rachel Bousfield, Johanna Nader, J. Wendi Bailey, Nicholas J. Beeching, Nicholas P. Day, Christopher M. Parry, William A Paxton
    PLOS ONE.2015; 10(5): e0123719.     CrossRef
  • Evolution, molecular epidemiology and perspectives on the research of taeniid parasites with special emphasis on Taenia solium
    Raúl J. Bobes, Gladis Fragoso, Agnès Fleury, Martín García-Varela, Edda Sciutto, Carlos Larralde, Juan P. Laclette
    Infection, Genetics and Evolution.2014; 23: 150.     CrossRef
  • The Parasitological, Immunological, and Molecular Diagnosis of Human Taeniasis with Special Emphasis on Taenia solium Taeniasis
    Kabemba E. Mwape, Sarah Gabriël
    Current Tropical Medicine Reports.2014; 1(4): 173.     CrossRef
  • Comparative analysis of the miRNA profiles from Taenia solium and Taenia asiatica adult
    Ai Lin, Chen Mu-Xin, Zhang Yong-Nian, Chen Shao-Hong, Zhou Xiao-Nong, Chen Jia-Xu
    African Journal of Microbiology Research.2014; 8(9): 895.     CrossRef
  • Prevalence of Intestinal Helminths among Inhabitants of Cambodia (2006-2011)
    Tai-Soon Yong, Jong-Yil Chai, Woon-Mok Sohn, Keeseon S. Eom, Hoo-Gn Jeoung, Eui-Hyug Hoang, Cheong-Ha Yoon, Bong-Kwang Jung, Soon-Hyung Lee, Muth Sinuon, Duong Socheat
    The Korean Journal of Parasitology.2014; 52(6): 661.     CrossRef
  • Epidemiology and genetic diversity of Taenia asiatica: a systematic review
    Anita Ale, Bjorn Victor, Nicolas Praet, Sarah Gabriël, Niko Speybroeck, Pierre Dorny, Brecht Devleesschauwer
    Parasites & Vectors.2014;[Epub]     CrossRef
  • Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections
    Jaco J. Verweij, C. Rune Stensvold
    Clinical Microbiology Reviews.2014; 27(2): 371.     CrossRef
  • Molecular Approaches to Taenia asiatica
    Hyeong-Kyu Jeon, Keeseon S. Eom
    The Korean Journal of Parasitology.2013; 51(1): 1.     CrossRef
  • 9,960 View
  • 117 Download
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CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax
Hyo-Jin Kim, Bong-Kwang Jung, Jin-Joo Lee, Kyoung-Ho Pyo, Tae Yun Kim, Byung-il Choi, Tae Woo Kim, Hajime Hisaeda, Kunisuke Himeno, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2011;49(1):85-90.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.85

Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.

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  • Live Vaccination with Blood-Stage Plasmodium yoelii 17XNL Prevents the Development of Experimental Cerebral Malaria
    Takashi Imai, Ha Ngo-Thanh, Kazutomo Suzue, Aoi Shimo, Akihiro Nakamura, Yutaka Horiuchi, Hajime Hisaeda, Takashi Murakami
    Vaccines.2022; 10(5): 762.     CrossRef
  • Immunotherapeutic Effects of Different Doses of Mycobacterium tuberculosis ag85a/b DNA Vaccine Delivered by Electroporation
    Yan Liang, Lei Cui, Li Xiao, Xiao Liu, Yourong Yang, Yanbo Ling, Tong Wang, Lan Wang, Jie Wang, Xueqiong Wu
    Frontiers in Immunology.2022;[Epub]     CrossRef
  • Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol
    Leoneide Érica Maduro Bouillet, Mariana Oliveira Dias, Natália Alves Dorigo, Andrew Douglas Moura, Bruce Russell, Francois Nosten, Laurent Renia, Érika Martins Braga, Ricardo Tostes Gazzinelli, Maurício M. Rodrigues, Irene S. Soares, Oscar Bruna-Romero, J
    Infection and Immunity.2011; 79(9): 3642.     CrossRef
  • 9,873 View
  • 74 Download
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Original Article

Molecular Variation and Distribution of Anopheles fluviatilis (Diptera: Culicidae) Complex in Iran
Saied Reza Naddaf, Mohammad Reza Razavi, Golnaz Bahramali
Korean J Parasitol 2010;48(3):231-236.
Published online September 16, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.3.231

Anopheles fluviatilis James (Diptera: Culicidae) is one of the known malaria vectors in south and southeastern Iran. Earlier ITS2 sequences analysis of specimens from Iran demonstrated only a single genotype that was identical to species Y in India, which is also the same as species T. We identified 2 haplotypes in the An. fluviatilis populations of Iran based on differences in nucleotide sequences of D3 domain of the 28S locus of ribosomal DNA (rDNA). Comparison of sequence data from 44 Iranian specimens with those publicly available in the Genbank database showed that all of the 28S-D3 sequences from Kazeroun and Khesht regions in Fars Province were identical to the database entry representing species U in India. In other regions, all the individuals showed heterozygosity at the single nucleotide position, which identifies species U and T. It is argued that the 2 species may co-occur in some regions and hybridize; however, the heterozygosity in the 28S-D3 locus was not reflected in ITS2 sequences and this locus for all individuals was identical to species T. This study shows that in a newly diverged species, like members of An. fluviatilis complex, a single molecular marker may not be sufficiently discriminatory to identify all the taxa over a vast geographical area. In addition, other molecular markers may provide more reliable information for species discrimination.

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  • Are members of the Anopheles fluviatilis complex conspecific?
    Om P. Singh, Ankita Sindhania, Gunjan Sharma, Shobhna Mishra, Surya K. Sharma, Piyoosh K. Singh, Manoj K. Das
    Acta Tropica.2021; 224: 106149.     CrossRef
  • A detailed review of the mosquitoes (Diptera: Culicidae) of Iran and their medical and veterinary importance
    Shahyad Azari-Hamidian, Behzad Norouzi, Ralph E. Harbach
    Acta Tropica.2019; 194: 106.     CrossRef
  • Spatial changes in the distribution of malaria vectors during the past 5 decades in Iran
    A. Salahi-Moghaddam, A. Khoshdel, H. Dalaei, K. Pakdad, G.G. Nutifafa, M.M. Sedaghat
    Acta Tropica.2017; 166: 45.     CrossRef
  • An evaluation of the suitability of COI and COII gene variation for reconstructing the phylogeny of, and identifying cryptic species in, anopheline mosquitoes (Diptera Culicidae)
    Gang Wang, Chunxiao Li, Wei Zheng, Fenglin Song, Xiaoxia Guo, Zhonghua Wu, Peng Luo, Yongyao Yang, Lei He, Tongyan Zhao
    Mitochondrial DNA Part A.2017; 28(5): 769.     CrossRef
  • An integrative approach to species discrimination in the Anagrus atomus group sensu stricto (Hymenoptera: Mymaridae), with a description of a new species
    Francesco Nugnes, Umberto Bernardo, Gennaro Viggiani
    Systematics and Biodiversity.2017; 15(6): 582.     CrossRef
  • Molecular Phylogenetic Analysis of the SubgeneraAnophelesandCellia(Diptera: Culicidae) Based on Nuclear Ribosomal Sequences
    G. Wang, C. Li, X. Guo, D. Xing, Y. Dong, T. Zhao
    African Entomology.2014; 22(3): 660.     CrossRef
  • Molecular phylogenetic analysis of Anopheles and Cellia subgenus anophelines (Diptera: Culicidae) in temperate and tropical regions of Iran
    Saber Gholizadeh, Navid Dinparast Djadid, Behzad Nouroozi, Mojtaba Bekmohammadi
    Acta Tropica.2013; 126(1): 63.     CrossRef
  • Mosquito fauna (Diptera: Culicidae) of the Iranian islands in the Persian Gulf II. Greater Tonb, Lesser Tonb and Kish Islands
    M. Khoobdel, S. Azari-Hamidian, A.A. Hanafi-Bojd
    Journal of Natural History.2012; 46(31-32): 1939.     CrossRef
  • Malaria elimination trend from a hypo-endemic unstable active focus in southern Iran: predisposing climatic factors
    M D Moemenbellah-Fard, V Saleh, O Banafshi, T Dabaghmanesh
    Pathogens and Global Health.2012; 106(6): 358.     CrossRef
  • Spatio—temporal distribution of malaria vectors (Diptera: Culicidae) across different climatic zones of Iran
    Ahmad Ali Hanafi-Bojd, Shahyad Azari-Hamidian, Vatandoost Hassan, Charrahy Zabihollah
    Asian Pacific Journal of Tropical Medicine.2011; 4(6): 498.     CrossRef
  • 12,248 View
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Mini Review

Molecular Phylogeny of Acanthamoeba
Hyun Hee Kong
Korean J Parasitol 2009;47(Suppl):S21.
Published online October 26, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.S.S21

After morphological grouping of Acanthamoeba by Pussard and Pons, phylogeny of the genus has been always a big topic to the researchers. Because of the variability of morphological characteristics, unchangeable and stable characters have been investigated for phylogenic criteria. Isoenzyme and mitochondrial DNA RFLP (Mt DNA RFLP) analyses revealed different patterns among strains assigned to a same species. Therefore, these characteristics would be considered as tools for strain discrimination than species identification. The most recently developed and the most promising method is the sequence analysis of 18s ribosomal RNA coding DNA (18s rDNA). The phylogenic tree based on comparison of 18s rDNA sequences distinguished the 3 morphological groups of Acanthamoeba and divided them into 12 unique sequence types (T1-T12 genotypes). Most clinical and environmental isolates belonged to the morphological group II and the genotype T4. In the Republic of Korea, 2 strains of Acanthamoeba, YM-2 and YM-3, were first isolated from the environment in 1974. However, phylogenic identification of Korean Acanthamoeba isolates from human infections or the environment were tried from the late 1990s. By RFLP analysis or total sequence analysis of 18s rDNA revealed that almost all clinical isolates including the one from a suspicious granulomatous amebic encephalitis patient belonged to the genotype T4. A large number of environmental isolates from contact lens storage cases, tapped water, and ocean sediments also belonged to the genotype T4. Almost identical strain characteristics, such as Mt DNA RFLP pattern of environmental isolates, with the clinical isolates could make a simple conclusion that most environmental isolates might be a potential keratopathogen.

Citations

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  • Insights Into the Genetic Diversity of Acanthamoeba Keratitis: A Clinical and Molecular Study
    Vinil K. Kavya, Lakshmipathy Dhanurekha, Ramachandran Abirami, Ranganathan Sampathkumar, Agarwal S. Shweta, Appakkudal R. Anand, Sri B. Madhangi
    Cornea.2025;[Epub]     CrossRef
  • Epidemiology of and Genetic Factors Associated with Acanthamoeba Keratitis
    Muhammad Ilyas, Fiona Stapleton, Mark D. P. Willcox, Fiona Henriquez, Hari Kumar Peguda, Binod Rayamajhee, Tasbiha Zahid, Constantinos Petsoglou, Nicole A. Carnt
    Pathogens.2024; 13(2): 142.     CrossRef
  • A Narrative Review of Acanthamoeba Isolates in Malaysia: Challenges in Infection Management and Natural Therapeutic Advancements
    Mohammad Wisman Abdul Hamid, Roslaini Bin Abd Majid, Victor Fiezal Knight Victor Ernest, Nik Noorul Shakira Mohamed Shakrin, Firdaus Mohamad Hamzah, Mainul Haque
    Cureus.2024;[Epub]     CrossRef
  • Assessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer
    Rohaya Abdul Halim, Hasseri Halim, Rosnani Hanim Mohd Hussain, Shafiq Aazmi, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar
    Journal of Water and Health.2024; 22(12): 2289.     CrossRef
  • Biological characteristics and pathogenicity of Acanthamoeba
    Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Shotgun Kinetic Target-Guided Synthesis Approach Enables the Discovery of Small-Molecule Inhibitors against Pathogenic Free-Living Amoeba Glucokinases
    Mintesinot Kassu, Prakash T. Parvatkar, Jillian Milanes, Neil P. Monaghan, Chungsik Kim, Matthew Dowgiallo, Yingzhao Zhao, Ami H. Asakawa, Lili Huang, Alicia Wagner, Brandon Miller, Karissa Carter, Kayleigh F. Barrett, Logan M. Tillery, Lynn K. Barrett, I
    ACS Infectious Diseases.2023; 9(11): 2190.     CrossRef
  • Molecular detection of free-living amoebae from Namhangang (southern Han River) in Korea
    Heekyoung Kang, Hae-Jin Sohn, Ga-Eun Seo, Gi-Sang Seong, A-Jeong Ham, A-Young Park, Suk-Yul Jung, Sang-Eun Lee, Shin-Hyeong Cho, Ho-Joon Shin
    Scientific Reports.2020;[Epub]     CrossRef
  • Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)
    Stas Malavin, Lyubov Shmakova
    European Journal of Protistology.2020; 73: 125671.     CrossRef
  • Isolation and molecular characterization of Acanthamoeba from patients with keratitis in Spain
    T. Martín-Pérez, A. Criado-Fornelio, J. Martínez, M.A. Blanco, I. Fuentes, J. Pérez-Serrano
    European Journal of Protistology.2017; 61: 244.     CrossRef
  • Free-living amoebae in the water resources of Iran: a systematic review
    Ehsan Saburi, Toktam Rajaii, Asma Behdari, Mohammad Hasan Kohansal, Hossein Vazini
    Journal of Parasitic Diseases.2017; 41(4): 919.     CrossRef
  • Genotyping of Acanthamoeba spp. from water sources from Northwestern Iran
    Ali Haniloo, Ali Pezeshki, Abbas Mahmmodzadeh, Elnaz Kadkhodamohammadi
    Acta Parasitologica.2017;[Epub]     CrossRef
  • Pathogenic free-living amoeba
    Natalia Łanocha-Arendarczyk, Danuta Kosik-Bogacka, Katarzyna Galant, Wojciech Zaorski, Karolina Kot, Aleksandra Łanocha
    Postępy Mikrobiologii - Advancements of Microbiology.2017; 56(1): 106.     CrossRef
  • Metacommunity analysis of amoeboid protists in grassland soils
    Anna Maria Fiore-Donno, Jan Weinert, Tesfaye Wubet, Michael Bonkowski
    Scientific Reports.2016;[Epub]     CrossRef
  • Genotypic, physiological, and biochemical characterization of potentially pathogenic Acanthamoeba isolated from the environment in Cairo, Egypt
    Gihan Mostafa Tawfeek, Sawsan Abdel-Hamid Bishara, Rania Mohammad Sarhan, Eman ElShabrawi Taher, Amira ElSaady Khayyal
    Parasitology Research.2016; 115(5): 1871.     CrossRef
  • Incidence and molecular diversity of Acanthamoeba species isolated from public baths in Hungary
    Csaba Kiss, Zsófia Barna, Márta Vargha, Júlia Katalin Török
    Parasitology Research.2014; 113(7): 2551.     CrossRef
  • Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
    Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
    The Korean Journal of Parasitology.2013; 51(4): 401.     CrossRef
  • Occurrence and characterization of Acanthamoeba similar to genotypes T4, T5, and T2/T6 isolated from environmental sources in Brasília, Federal District, Brazil
    Daniella de Sousa Mendes Moreira Alves, Aline Silva Moraes, Nadjar Nitz, Mayara Gabriele Carvalho de Oliveira, Mariana Machado Hecht, Rodrigo Gurgel-Gonçalves, César Augusto Cuba Cuba
    Experimental Parasitology.2012; 131(2): 239.     CrossRef
  • Exploring the Unique N-Glycome of the Opportunistic Human Pathogen Acanthamoeba
    Birgit Schiller, Georgia Makrypidi, Ebrahim Razzazi-Fazeli, Katharina Paschinger, Julia Walochnik, Iain B.H. Wilson
    Journal of Biological Chemistry.2012; 287(52): 43191.     CrossRef
  • Comparison of molecular diagnostic methods for the detection of Acanthamoeba spp. from clinical specimens submitted for keratitis
    Krishna Khairnar, Gurdip S. Tamber, Filip Ralevski, Dylan R. Pillai
    Diagnostic Microbiology and Infectious Disease.2011; 70(4): 499.     CrossRef
  • 19,262 View
  • 194 Download
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Original Article

Imported Malaria in United Arab Emirates: Evaluation of a New DNA Extraction Technique Using Nested PCR
Doaa M. Sultan, Marwa M. Khalil, Ahmed S. Abdouh, Wafaa F. Doleh, Abdul Aziz M. Al Muthanna
Korean J Parasitol 2009;47(3):227-233.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.227

Local malaria transmission in the United Arab Emirates (UAE) came to an end in 1997. Nevertheless, UAE has been subjected to substantial importation of malaria cases from abroad, concerning both UAE nationals and immigrants from malarious countries with a total number of 2,119 cases in 2007. To evaluate a new DNA extraction technique using nested PCR, blood samples were collected from 132 individuals who presented to Infectious Diseases Department in Rashid Hospital, Dubai, and Central Department of Malaria Control with fever and persistent headache. Giemsa-stained blood films and ELISA test for malaria antibodies were carried out for detection of Plasmodium infection. Plasmodium infections were identified with the genus-specific primer set and species differentiation using nested PCR. A rapid procedure for diagnosis of malaria infections directly from dried blood spots using for the first time DNA extract from FTA Elute cards was evaluated in contrast to extraction techniques using FTA classic cards and rapid boiling technique. Our new simple technique for DNA extraction using FTA Elute cards was very sensitive giving a sensitivity of 100% compared to 94% using FTA classic cards and 62% in the rapid boiling technique. No complex preparation of blood samples was required prior to the amplification. The production cost of DNA isolation in our PCR assay was much less in comparable to that of other DNA extraction protocols. The nested PCR detected plasmodial infection and could differentiate P. falciparum from P. vivax, and also detected the mixed infection.

Citations

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  • Nucleic acid purification from dried blood spot on FTA Elute Card provides template for polymerase chain reaction for highly sensitive Plasmodium detection
    Muneaki Hashimoto, Mika Bando, Jun-ichi Kido, Kazumichi Yokota, Toshihiro Mita, Kazuaki Kajimoto, Masatoshi Kataoka
    Parasitology International.2019; 73: 101941.     CrossRef
  • Genetic diversity and transmissibility of imported Plasmodium vivax in Qatar and three countries of origin
    Mohammed H. Abdelraheem, Devendra Bansal, Mohammed A. Idris, Moawia M. Mukhtar, Muzamil M. Abdel Hamid, Zainb S. Imam, Sisay Getachew, Rakesh Sehgal, Hargobinder Kaur, Amal H. Gadalla, Salam Al-Hamidhi, Zainab Al-Hashami, Ali Al-Jabri, Ali A. Sultan, Hamz
    Scientific Reports.2018;[Epub]     CrossRef
  • A simple, efficient and inexpensive method for malaria parasites’ DNA catching from fixed Giemsa-stained blood slides
    Abbas Ali Eskandarian, Sara Moradi, Saeed Abedi
    Journal of Parasitic Diseases.2016; 40(3): 833.     CrossRef
  • Development of new malaria diagnostics: matching performance and need
    David Bell, Alessandra E. Fleurent, Michael C. Hegg, John D. Boomgard, Caitlin C. McConnico
    Malaria Journal.2016;[Epub]     CrossRef
  • Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination
    E. C. Oriero, J. Jacobs, J.-P. Van Geertruyden, D. Nwakanma, U. D'Alessandro
    Journal of Antimicrobial Chemotherapy.2015; 70(1): 2.     CrossRef
  • Genetic diversity of Plasmodium falciparum and distribution of drug resistance haplotypes in Yemen
    Salama Al-Hamidhi, Mohammed AK Mahdy, Zainab Al-Hashami, Hissa Al-Farsi, Abdulsalam M Al-mekhlafi, Mohamed A Idris, Albano Beja-Pereira, Hamza A Babiker
    Malaria Journal.2013;[Epub]     CrossRef
  • Quantitative detection of <i>Streptococcus mutans</i> from saliva using FTA<sup>TM</sup> elute cards and real-time polymerase chain reaction
    Sepideh Seghatoleslami, Lars Ohlsson, Kristina Hamberg, Peter Carlsson, Dan Ericson, Lennart Ljunggren
    American Journal of Molecular Biology.2013; 03(03): 148.     CrossRef
  • An optimized method for elution of enteroviral RNA from a cellulose-based substrate
    Yan Li, Hiromu Yoshida, Lu Wang, Zexin Tao, Haiyan Wang, Xiaojuan Lin, Aiqiang Xu
    Journal of Virological Methods.2012; 186(1-2): 62.     CrossRef
  • Comparative Analytical Utility of DNA Derived from Alternative Human Specimens for Molecular Autopsy and Diagnostics
    Tara L. Klassen, Eva-Lotta von Rüden, Janice Drabek, Jeffrey L. Noebels, Alica M. Goldman
    The Journal of Molecular Diagnostics.2012; 14(5): 451.     CrossRef
  • Source of drug resistant Plasmodium falciparum in a potential malaria elimination site in Saudi Arabia
    Hissa M. Al-Farsi, Zainab S. Al-Hashami, Saad M. Bin Dajem, Adel Ali H. Al-Sheikh, Ahmed Al-Qahtani, Albano Beja-Pereira, Mohamed A. Idris, Hamza A. Babiker
    Infection, Genetics and Evolution.2012; 12(6): 1253.     CrossRef
  • 9,695 View
  • 84 Download
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Case Report

A Case of Fatal Strongyloidiasis in a Patient with Chronic Lymphocytic Leukemia and Molecular Characterization of the Isolate
Eshrat Beigom Kia, Hamid Reza Rahimi, Hossein Mirhendi, Mohammad Reza Nilforoushan, Ardeshir Talebi, Farzaneh Zahabiun, Hamid Kazemzadeh, Ahmad Reza Meamar
Korean J Parasitol 2008;46(4):261-263.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.261

Strongyloides stercoralis is a human intestinal parasite which may lead to complicated strongyloidiasis in immunocompromised. Here, a case of complicated strongyloidiasis in a patient with chronic lymphocytic leukemia is reported. Presence of numerous S. stercoralis larvae in feces and sputum confirmed the diagnosis of hyperinfection syndrome in this patient. Following recovery of filariform larvae from agar plate culture of the stool, the isolate was characterized for the ITS1 region of ribosomal DNA gene by nested-PCR and sequencing. Albendazole therapy did not have cure effects; and just at the beginning of taking ivermectin, the patient died. The most important clue to prevent such fatal consequences is early diagnosis and proper treatment.

Citations

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  • Multifaceted clinical presentations and predisposing factors of Strongyloides stercoralis and its treatment outcome: A case series
    Subhayan Das Gupta, Rohon Das Roy, Dipmala Das
    Tropical Doctor.2025; 55(4): 339.     CrossRef
  • Severe strongyloidiasis: a systematic review and meta-analysis of 339 cases
    Olga C Rojas, Alexandra M Montoya, Hiram Villanueva-Lozano, Diego Carrion-Alvarez
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2023; 117(10): 682.     CrossRef
  • Fatal Disseminated Strongyloidiasis in an Immunosuppressed Patient During COVID-19 Pandemic
    Eissa Soleymani, Lotfollah Davoodi, Shadi Shayesteh Azar, Seyed Reza Mirbadiei, Fatemeh Parandin, Afshin Azimi, Azadeh Mizani, Zohreh Khorshidvand, Mahdi Fakhar
    Acta Parasitologica.2023; 68(3): 711.     CrossRef
  • Molecular characterization of human isolates of Strongyloides stercoralis and Rhabditis spp. based on mitochondrial cytochrome c oxidase subunit 1 (cox1)
    Mandana Fadaei Tehrani, Meysam Sharifdini, Farzaneh Zahabiun, Robabeh Latifi, Eshrat Beigom Kia
    BMC Infectious Diseases.2019;[Epub]     CrossRef
  • Strongyloidiasis in northern Vietnam: epidemiology, clinical characteristics and molecular diagnosis of the causal agent
    Nguyen Van De, Pham Ngoc Minh, Le Van Duyet, Santiago Mas-Coma
    Parasites & Vectors.2019;[Epub]     CrossRef
  • Effects of dexamethasone, cyclosporine and betamethasone on inflammatory cell recruitment in mice infected with Strongyloides venezuelensis
    Eleuza R Machado, Marlene T Ueta, Rosângela Maria Rodrigues, Simone G Ramos, Virgínia Vilhena, Anna Maly de Leão e Neves Eduardo, Leandro Junio Barreto dos Reis, Raphael da Silva Affonso, Lúcia Helena Faccioli
    Journal of Lung, Pulmonary & Respiratory Research.2019; 6(4): 108.     CrossRef
  • Strongyloides stercoralis hyperinfection in an unconscious diabetic patient with dermatomyositis
    Meysam Sharifdini, Aniseh Hesari, SeifAli Mahdavi, Akram Alipour, EshratBeigom Kia
    Indian Journal of Pathology and Microbiology.2018; 61(1): 109.     CrossRef
  • Strongyloidiasis Current Status with Emphasis in Diagnosis and Drug Research
    Tiago Mendes, Karen Minori, Marlene Ueta, Danilo Ciccone Miguel, Silmara Marques Allegretti
    Journal of Parasitology Research.2017; 2017: 1.     CrossRef
  • Case Report: Strongyloides stercoralis Hyperinfection in a Patient with Chronic Lymphocytic Leukemia
    Aimee Wilkin, Elizabeth Palavecino, Richelle Guerrero-Wooley, Ernesto Aranda-Aguirre, Wencheng Li
    The American Journal of Tropical Medicine and Hygiene.2017; 97(5): 1629.     CrossRef
  • A PCR-Based Molecular Detection of Strongyloides stercoralis in Human Stool Samples from Tabriz City, Iran
    Reza Ghasemikhah, Mohammad Tabatabaiefar, Seyed Shariatzadeh, Abbas Shahbazi, Teymour Hazratian
    Scientia Pharmaceutica.2017; 85(2): 17.     CrossRef
  • Seroepidemiology of Strongyloides stercoralis amongst immunocompromised patients in Southwest Iran
    Reza Rafiei, Abdollah Rafiei, Mahmoud Rahdar, Bijan Keikhaie
    Parasite Epidemiology and Control.2016; 1(3): 229.     CrossRef
  • Strong-LAMP: A LAMP Assay for Strongyloides spp. Detection in Stool and Urine Samples. Towards the Diagnosis of Human Strongyloidiasis Starting from a Rodent Model
    Pedro Fernández-Soto, Alicia Sánchez-Hernández, Javier Gandasegui, Cristina Bajo Santos, Julio López-Abán, José María Saugar, Esperanza Rodríguez, Belén Vicente, Antonio Muro, James S McCarthy
    PLOS Neglected Tropical Diseases.2016; 10(7): e0004836.     CrossRef
  • Single Donor-Derived Strongyloidiasis in Three Solid Organ Transplant Recipients: Case Series and Review of the Literature
    M. Le, K. Ravin, A. Hasan, H. Clauss, D.G. Muchant, J.K. Pasko, G. Cipollina, F. Abanyie, S.P. Montgomery, M. Loy, M. Ahmed, M. Mathur, B. Chokkalingam Mani, J. Mehr, A. Kotru, C. Varma, M. Maksimak, M. Schultz, G. Obradovic, R. Alvarez, Y. Toyoda, M. Bir
    American Journal of Transplantation.2014; 14(5): 1199.     CrossRef
  • Fatal Strongyloides hyper-infection in a patient with myasthenia gravis
    M. Saraei, B. Hosseinbigi, M. Shahnazi, B. Bijani
    Infection.2014; 42(6): 1039.     CrossRef
  • Severe strongyloidiasis: a systematic review of case reports
    Dora Buonfrate, Ana Requena-Mendez, Andrea Angheben, Jose Muñoz, Federico Gobbi, Jef Van Den Ende, Zeno Bisoffi
    BMC Infectious Diseases.2013;[Epub]     CrossRef
  • Fulminant gastrointestinal hemorrhage due to Strongyloides stercoralis hyperinfection in an AIDS patient
    Valdes Roberto Bollela, Cinara Feliciano, André Costa Teixeira, Ana Carolina Ribeiro Junqueira, Marcos Antonio Rossi
    Revista da Sociedade Brasileira de Medicina Tropical.2013; 46(1): 111.     CrossRef
  • Strongyloidiasis: An Emerging Infectious Disease in China
    Xiaohong Zhou, Anthony A. James, Chunmei Wang, Juan Li, Xiaoguang Chen, Guiyun Yan, Jiabao Xu
    The American Journal of Tropical Medicine and Hygiene.2013; 88(3): 420.     CrossRef
  • Prevalence of Strongyloides stercoralis and other intestinal parasitic infections among mentally retarded residents in central institution of southern Iran
    Azar Shokri, Khojasteh Sharifi Sarasiabi, Saeed Hosseini Teshnizi, Hamid Mahmoodi
    Asian Pacific Journal of Tropical Biomedicine.2012; 2(2): 88.     CrossRef
  • 8,494 View
  • 102 Download
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Original Article

Interaction between Parasitophorous Vacuolar Membrane-associated GRA3 and Calcium Modulating Ligand of Host Cell Endoplasmic Reticulum in the Parasitism of Toxoplasma gondii
Ji Yeon Kim, Hye-Jin Ahn, Kyung Ju Ryu, Ho-Woo Nam
Korean J Parasitol 2008;46(4):209-216.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.209

A monoclonal antibody against Toxoplasma gondii of Tg556 clone (Tg556) blotted a 29 kDa protein, which was localized in the dense granules of tachyzoites and secreted into the parasitophorous vacuolar membrane (PVM) after infection to host cells. A cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg556, and the full-length was completed by 5'-RACE of 2,086 bp containing an open reading frame (ORF) of 669 bp. The ORF encoded a polypeptide of 222 amino acids homologous to the revised GRA3 but not to the first reported one. The polypeptide has 3 hydrophobic moieties of an N-terminal stop transfer sequence and 2 transmembrane domains (TMD) in posterior half of the sequence, a cytoplasmic localization motif after the second TMD and an endoplasmic reticulum (ER) retrival motif in the C-terminal end, which suggests GRA3 as a type III transmembrane protein. With the ORF of GRA3, yeast two-hybrid assay was performed in HeLa cDNA expression library, which resulted in the interaction of GRA3 with calcium modulating ligand (CAMLG), a type II transmembrane protein of ER. The specific binding of GRA3 and CAMLG was confirmed by glutathione S-transferase (GST) pull-down and immunoprecipitation assays. The localities of fluorescence transfectionally expressed from GRA3 and CAMLG plasmids were overlapped completely in HeLa cell cytoplasm. In immunofluorescence assay, GRA3 and CAMLG were shown to be co-localized in the PVM of host cells. Structural binding of PVM-inserted GRA3 to CAMLG of ER suggested the receptor-ligand of ER recruitment to PVM during the parasitism of T. gondii.

Citations

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  • Toxoplasma effector TgROP1 establishes membrane contact sites with the endoplasmic reticulum during infection
    Chahat Mehra, Jesús Alvarado Valverde, Ana Margarida Nogueira Matias, Francesca Torelli, Tânia Catarina Medeiros, Julian Straub, James D. Asaki, Peter J. Bradley, Katja Luck, Steffen Lawo, Moritz Treeck, Lena Pernas
    Nature Microbiology.2025; 10(12): 3331.     CrossRef
  • Toxoplasma–host endoplasmic reticulum interaction: How T. gondii activates unfolded protein response and modulates immune response
    Obed Cudjoe, Roger Afful, Tonny Abraham Hagan
    Current Research in Microbial Sciences.2024; 6: 100223.     CrossRef
  • Host cell manipulation by microsporidia secreted effectors: Insights into intracellular pathogenesis
    Liyuan Tang, Musa Makongoro Sabi, Ming Fu, Jingyu Guan, Yongliang Wang, Tian Xia, Kai Zheng, Hongnan Qu, Bing Han
    Journal of Eukaryotic Microbiology.2024;[Epub]     CrossRef
  • Preparation and Preliminary Application of Epitope Peptide-Based Antibody against Toxoplasma gondii GRA3
    Ru Wang, Minmin Wu, Haijian Cai, Ran An, Ying Chen, Jie Wang, Nan Zhou, Jian Du
    Tropical Medicine and Infectious Disease.2023; 8(3): 143.     CrossRef
  • Intracellular life of protozoan Toxoplasma gondii: Parasitophorous vacuole establishment and survival strategies
    JULIANA A. PORTES, ROSSIANE C. VOMMARO, LUCIO AYRES CALDAS, ERICA S. MARTINS-DUARTE
    BIOCELL.2023; 47(4): 929.     CrossRef
  • A state-of-the-art methodology for high-throughput in silico vaccine discovery against protozoan parasites and exemplified with discovered candidates for Toxoplasma gondii
    Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis
    Scientific Reports.2023;[Epub]     CrossRef
  • Toxoplasma gondii dense granule protein 3 promotes endoplasmic reticulum stress-induced apoptosis by activating the PERK pathway
    Cudjoe Obed, Minmin Wu, Ying Chen, Ran An, Haijian Cai, Qingli Luo, Li Yu, Jie Wang, Fang Liu, Jilong Shen, Jian Du
    Parasites & Vectors.2022;[Epub]     CrossRef
  • The UPR sensor IRE1α promotes dendritic cell responses to control Toxoplasma gondii infection
    Anaïs F Poncet, Victor Bosteels, Eik Hoffmann, Sylia Chehade, Sofie Rennen, Ludovic Huot, Véronique Peucelle, Sandra Maréchal, Jamal Khalife, Nicolas Blanchard, Sophie Janssens, Sabrina Marion
    EMBO reports.2021;[Epub]     CrossRef
  • Interorganellar communication and membrane contact sites in protozoan parasites
    Herbert J. Santos, Tomoyoshi Nozaki
    Parasitology International.2021; 83: 102372.     CrossRef
  • A Newly Discovered Dense Granule Protein 3 in Neospora caninum
    Panpan Zhao, Jingquan Dong, Lili Cao, Jianhua Li, Xichen Zhang, Xin Li, Xiaocen Wang, Babatunde Kazeem Bello, Nan Zhang, Pengtao Gong
    Acta Parasitologica.2021; 66(4): 1581.     CrossRef
  • Manipulation of Host Cell Organelles by Intracellular Pathogens
    Malte Kellermann, Felix Scharte, Michael Hensel
    International Journal of Molecular Sciences.2021; 22(12): 6484.     CrossRef
  • Toxoplasma gondii Parasitophorous Vacuole Membrane-Associated Dense Granule Proteins Regulate Maturation of the Cyst Wall
    Rebekah B. Guevara, Barbara A. Fox, David J. Bzik, Silvia N. J. Moreno
    mSphere.2020;[Epub]     CrossRef
  • TheToxoplasma gondiidense granule protein TgGRA3 interacts with host Golgi and dysregulates anterograde transport
    Maika S. Deffieu, Tchilabalo Dilezitoko Alayi, Christian Slomianny, Stanislas Tomavo
    Biology Open.2019;[Epub]     CrossRef
  • Rhoptry and Dense Granule Secreted Effectors Regulate CD8+ T Cell Recognition of Toxoplasma gondii Infected Host Cells
    Leah M. Rommereim, Barbara A. Fox, Kiah L. Butler, Viviana Cantillana, Gregory A. Taylor, David J. Bzik
    Frontiers in Immunology.2019;[Epub]     CrossRef
  • Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system
    Fa-Cai Li, Qing Liu, Hany M. Elsheikha, Wen-Bin Yang, Jun-Ling Hou, Xing-Quan Zhu
    Parasitology Research.2018; 117(4): 1291.     CrossRef
  • The ER phagosome connection in the era of membrane contact sites
    Paula Nunes-Hasler, Nicolas Demaurex
    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research.2017; 1864(9): 1513.     CrossRef
  • How Toxoplasma and malaria parasites defy first, then exploit host autophagic and endocytic pathways for growth
    Isabelle Coppens
    Current Opinion in Microbiology.2017; 40: 32.     CrossRef
  • Genotyping of polymorphic effectors of Toxoplasma gondii isolates from China
    Weisheng Cheng, Cong Wang, Ting Xu, Fang Liu, Faustina Pappoe, Qingli Luo, Yuanhong Xu, Fangli Lu, Jilong Shen
    Parasites & Vectors.2017;[Epub]     CrossRef
  • Apicomplexans pulling the strings: manipulation of the host cell cytoskeleton dynamics
    RITA CARDOSO, HELENA SOARES, ANDREW HEMPHILL, ALEXANDRE LEITÃO
    Parasitology.2016; 143(8): 957.     CrossRef
  • Variation detection based on next-generation sequencing of type Chinese 1 strains of Toxoplasma gondii with different virulence from China
    Weisheng Cheng, Fang Liu, Man Li, Xiaodong Hu, He Chen, Faustina Pappoe, Qingli Luo, Huiqin Wen, Tian Xing, Yuanhong Xu, Jilong Shen
    BMC Genomics.2015;[Epub]     CrossRef
  • Toxoplasmosis and Epilepsy — Systematic Review and Meta Analysis
    Edgard B. Ngoungou, Devender Bhalla, Amandine Nzoghe, Marie-Laure Dardé, Pierre-Marie Preux, Rima McLeod
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    Eukaryotic Cell.2015; 14(5): 454.     CrossRef
  • Phylogeny and virulence divergency analyses of Toxoplasma gondii isolates from China
    Min Li, Xu-Wei Mo, Lin Wang, He Chen, Qing-Li Luo, Hui-Qin Wen, Wei Wei, Ai-Mei Zhang, Jian Du, Fang-Li Lu, Zhao-Rong Lun, Ji-Long Shen
    Parasites & Vectors.2014;[Epub]     CrossRef
  • High Expression of Water-Soluble Recombinant Antigenic Domains ofToxoplasma gondii Secretory Organelles
    Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2014; 52(4): 367.     CrossRef
  • Exploitation of auxotrophies and metabolic defects in Toxoplasma as therapeutic approaches
    Isabelle Coppens
    International Journal for Parasitology.2014; 44(2): 109.     CrossRef
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    Ji Su Ma, Miwa Sasai, Jun Ohshima, Youngae Lee, Hironori Bando, Kiyoshi Takeda, Masahiro Yamamoto
    Journal of Experimental Medicine.2014; 211(10): 2013.     CrossRef
  • Discovering a vaccine against neosporosis using computers: is it feasible?
    Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis
    Trends in Parasitology.2014; 30(8): 401.     CrossRef
  • Host Organelle Hijackers: a similarmodus operandiforToxoplasma gondiiandChlamydia trachomatis: co-infection model as a tool to investigate pathogenesis
    Julia D. Romano, Isabelle Coppens
    Pathogens and Disease.2013; 69(2): 72.     CrossRef
  • Interactions of pathogen-containing compartments with the secretory pathway
    Johnathan Canton, Peter E. Kima
    Cellular Microbiology.2012; 14(11): 1676.     CrossRef
  • Transcriptomic Analysis of Toxoplasma Development Reveals Many Novel Functions and Structures Specific to Sporozoites and Oocysts
    Heather M. Fritz, Kerry R. Buchholz, Xiucui Chen, Blythe Durbin-Johnson, David M. Rocke, Patricia A. Conrad, John C. Boothroyd, Silvia N. Moreno
    PLoS ONE.2012; 7(2): e29998.     CrossRef
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    Fahad Haroon, Ulrike Händel, Frank Angenstein, Jürgen Goldschmidt, Peter Kreutzmann, Holger Lison, Klaus-Dieter Fischer, Henning Scheich, Wolfram Wetzel, Dirk Schlüter, Eike Budinger, Silvia N. Moreno
    PLoS ONE.2012; 7(4): e35516.     CrossRef
  • Toxoplasma gondii: Expression of GRA1 gene in endoplasmic reticulum promotes both growth and adherence and modulates intracellular calcium release in macrophages
    Jie Lin, Xu Lin, Guo-hua Yang, Yong Wang, Bi-wen Peng, Jian-yin Lin
    Experimental Parasitology.2010; 125(2): 165.     CrossRef
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    Wandong Zhang, Katerina V. Savelieva, Adisak Suwanichkul, Daniel L. Small, Laura L. Kirkpatrick, Nianhua Xu, Thomas H. Lanthorn, Gui-Lan Ye, Georges Chapouthier
    PLoS ONE.2010; 5(6): e11261.     CrossRef
  • GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane
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    The Korean Journal of Parasitology.2009; 47(Suppl): S29.     CrossRef
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Brief Communication

Identification of parasite DNA in common bile duct stones by PCR and DNA sequencing
Ji Sun Jang, Kyung Ho Kim, Jae-Ran Yu, Soo-Ung Lee
Korean J Parasitol 2007;45(4):301-306.
Published online December 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.4.301

We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones and 5 gallbladder (GB) stones. The patients who had the CBD stones suffered from cholangitis, and the patients with GB stones showed acute cholecystitis, respectively. The 28S and 18S rDNA genes were amplified successfully from 3 and/or 1 common bile duct stone samples, and then cloned and sequenced. The 28S and 18S rDNA sequences were highly conserved among isolates. Identity of the obtained 28S D1 rDNA with that of Clonorchis sinensis was higher than 97.6%, and identity of the 18S rDNA with that of other Ascarididae was 97.9%. Almost no intra-specific variations were detected in the 28S and 18S rDNA with the exception of a few nucleotide variations, i.e., substitution and deletion. These findings suggest that C. sinensis and Ascaris lumbricoides may be related with the biliary stone formation and development.

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  • Clonorchis sinensis infection are associated with calcium phosphate gallbladder stones: A single-center retrospective observational study in China
    Rui-Hong Ma, Xiao-Bing Luo, Yu Peng Liu, Qin Li
    Medicine.2025; 104(46): e45739.     CrossRef
  • Gallstone and Gallbladder Disease: Biliary Tract and Cholangiopathies
    Ludovica Ceci, Yuyan Han, Kelsey Krutsinger, Leonardo Baiocchi, Nan Wu, Debjyoti Kundu, Konstantina Kyritsi, Tianhao Zhou, Eugenio Gaudio, Heather Francis, Gianfranco Alpini, Lindsey Kennedy
    Comprehensive Physiology.2023; 13(3): 4909.     CrossRef
  • A systematic study on 33 gallbladder stones resembling adult Clonorchis sinensis worms
    R.-h. Ma, X.-f. Wang, Q. Li, H.-y. Cai, X.-b. Luo
    Journal of Helminthology.2022;[Epub]     CrossRef
  • Clonorcis sinensis eggs are associated with calcium carbonate gallbladder stones
    Tie Qiao, Rui-hong Ma, Zhen-liang Luo, Liu-qing Yang, Xiao-bing Luo, Pei-ming Zheng
    Acta Tropica.2014; 138: 28.     CrossRef
  • Molecular diagnosis in clinical parasitology: When and why?
    Samson SY Wong, Kitty SC Fung, Sandy Chau, Rosana WS Poon, Sally CY Wong, Kwok-Yung Yuen
    Experimental Biology and Medicine.2014; 239(11): 1443.     CrossRef
  • A microstructural study of gallbladder stones using scanning electron microscopy
    Tie Qiao, Rui‐Hong MA, Xiao‐Bing Luo, Zhen‐Liang Luo, Pei‐Ming Zheng, Liu‐Qing Yang
    Microscopy Research and Technique.2013; 76(5): 443.     CrossRef
  • The zoonotic, fish-borne liver flukes Clonorchis sinensis, Opisthorchis felineus and Opisthorchis viverrini
    Trevor N. Petney, Ross H. Andrews, Weerachai Saijuntha, Alexandra Wenz-Mücke, Paiboon Sithithaworn
    International Journal for Parasitology.2013; 43(12-13): 1031.     CrossRef
  • Molecular Diagnostics and Parasitic Disease
    Shawn Vasoo, Bobbi S. Pritt
    Clinics in Laboratory Medicine.2013; 33(3): 461.     CrossRef
  • Microscopic Examination of Gallbladder Stones Improves Rate of Detection of Clonorchis sinensis Infection
    Tie Qiao, Rui-hong Ma, Xiao-bing Luo, Pei-ming Zheng, Zhen-liang Luo, Liu-qing Yang
    Journal of Clinical Microbiology.2013; 51(8): 2551.     CrossRef
  • New pathophysiological concepts underlying pathogenesis of pigment gallstones
    Libor Vítek, Martin C. Carey
    Clinics and Research in Hepatology and Gastroenterology.2012; 36(2): 122.     CrossRef
  • Development of a real-time PCR assay for the detection of Clonorchis sinensis DNA in gallbladder bile and stone samples from patients with cholecystolithiasis
    Tie Qiao, Pei-Ming Zheng, Rui-Hong Ma, Xiao-Bing Luo, Zhen-Liang Luo
    Parasitology Research.2012; 111(4): 1497.     CrossRef
  • Genomics and molecular genetics of Clonorchis sinensis: Current status and perspectives
    Si-Yang Huang, Guang-Hui Zhao, Bao-Quan Fu, Min-Jun Xu, Chun-Ren Wang, Song-Ming Wu, Feng-Cai Zou, Xing-Quan Zhu
    Parasitology International.2012; 61(1): 71.     CrossRef
  • Trematode Infections
    Thomas Fürst, Urs Duthaler, Banchop Sripa, Jürg Utzinger, Jennifer Keiser
    Infectious Disease Clinics of North America.2012; 26(2): 399.     CrossRef
  • Concept of the pathogenesis and treatment of cholelithiasis
    Vasiliy Ivanovich Reshetnyak
    World Journal of Hepatology.2012; 4(2): 18.     CrossRef
  • Update on the diagnosis and treatment of food-borne trematode infections
    Jennifer Keiser, Urs Duthaler, Jürg Utzinger
    Current Opinion in Infectious Diseases.2010; 23(5): 513.     CrossRef
  • 8,472 View
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Original Articles

Molecular phylogeny of parasitic Platyhelminthes based on sequences of partial 28S rDNA D1 and mitochondrial cytochrome c oxidase subunit I
Soo-Ung Lee, Ha-Chung Chun, Sun Huh
Korean J Parasitol 2007;45(3):181-189.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.181

The phylogenic relationships existing among 14 parasitic Platyhelminthes in the Republic of Korea were investigated via the use of the partial 28S ribosomal DNA (rDNA) D1 region and the partial mitochondrial cytochrome c oxidase subunit 1 (mCOI) DNA sequences. The nucleotide sequences were analyzed by length, G + C %, nucleotide differences and gaps in order to determine the analyzed phylogenic relationships. The phylogenic patterns of the 28S rDNA D1 and mCOI regions were closely related within the same class and order as analyzed by the PAUP 4.0 program, with the exception of a few species. These findings indicate that the 28S rDNA gene sequence is more highly conserved than are the mCOI gene sequences. The 28S rDNA gene may prove useful in studies of the systematics and population genetic structures of parasitic Platyhelminthes.

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  • Ultrastructural and phylogenetic characterization of some trematode cercariae emerging from the freshwater ampullariid snail, Lanistes carinatus (Olivier, 1804)
    Mohammed E. El-Zeiny, Ahmed M. Ghoneim, Ola A. Abu Samak, Abdel Aziz A. Khidr
    Parasitology International.2025; 109: 103113.     CrossRef
  • Seasonal dynamics and molecular phylogenetic studies on cercariae in Central Zone of Kashmir valley
    Zahoor Ahmad Wani, Rafiq A. Shahardar, Kamal H. Bulbul, Idrees M. Allaie, Showkat A. Shah, Shabir A. Rather, Aiman Ashraf, Shahana Tramboo, Asif H. Khan, Shawky M Aboelhadid
    PLOS One.2025; 20(7): e0325160.     CrossRef
  • Deciphering the global genetic structure of Spirometra mansoni and S. erinaceieuropaei based on 28S ribosomal RNA: Insights into taxonomical revaluation and population dynamics
    Anil Kumar Nehra, Rasmita Panda, Prem Sagar Maurya, Ansu Kumari, Aman Dev Moudgil
    Parasitology Research.2025;[Epub]     CrossRef
  • Identification of Hydatigera Species in Wildcats (Felis silvestris) from Central Spain
    Pablo Matas-Méndez, Lorena Esteban-Sánchez, Francisco Ponce-Gordo, Marta Mateo-Barrientos
    Animals.2025; 15(22): 3340.     CrossRef
  • The infection of Cysticercus fasciolaris in natural rats (Rattus species) residing in human residence areas, Nakhon Si Thammarat, Thailand
    Sunsaneeya Thaikoed, Watcharapong Mitsuwan, Kittipong Chaisiri, Noppharat Tanthanathipchai, Maria de Lourdes Pereira, Alok K. Paul, Veeranoot Nissapatorn, Phirabhat Saengsawang
    Comparative Immunology, Microbiology and Infectious Diseases.2024; 107: 102152.     CrossRef
  • Case report: Disseminated larval trematodiasis caused by Clinostomum marginatum in a green tree frog (Hyla cinerea)
    Håkon Jones, Heather M.A. Fenton, Elizabeth J. Elsmo, Nicole M. Nemeth, Kayla B. Garrett, Christopher A. Cleveland, Michael J. Yabsley
    Veterinary Parasitology: Regional Studies and Reports.2024; 52: 101051.     CrossRef
  • Current status of intestinal parasitosis and microsporidiosis in industrialized countries: Results from a prospective study in France and Luxembourg
    Maxime Moniot, Céline Nourrisson, Eloïse Bailly, Céline Lambert, Patricia Combes, Philippe Poirier, María Victoria Periago
    PLOS Neglected Tropical Diseases.2024; 18(12): e0012752.     CrossRef
  • Fatal infection with Versteria sp. in a muskrat, with implications for human health
    Kevin D. Niedringhaus, Laken S. Ganoe, Matthew Lovallo, W. David Walter, Michael J. Yabsley, Justin D. Brown
    Journal of Veterinary Diagnostic Investigation.2022; 34(2): 314.     CrossRef
  • Expansion of Cyclophyllidea Biodiversity in Rodents of Qinghai-Tibet Plateau and the “Out of Qinghai-Tibet Plateau” Hypothesis of Cyclophyllideans
    Yao-Dong Wu, Guo-Dong Dai, Li Li, D. Timothy J. Littlewood, John Asekhaen Ohiolei, Lin-Sheng Zhang, Ai-Min Guo, Yan-Tao Wu, Xing-Wei Ni, Nigus Abebe Shumuye, Wen-Hui Li, Nian-Zhang Zhang, Bao-Quan Fu, Yong Fu, Hong-Bin Yan, Wan-Zhong Jia
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Morphological and molecular phylogenetic analysis of Bivesicula claviformis Yamaguti, 1934 infecting the tomato hind Cephalopholis sonnerati (Serranidae) in Saudi Arabia
    Jawahir Alghamdi, Saleh Al-Quraishy, Rewaida Abdel-Gaber
    Journal of King Saud University - Science.2022; 34(4): 101936.     CrossRef
  • Morphological and molecular analyses of Paropecoelus saudiae sp. nov. (Plagiorchiida: Opecoelidae), a trematoda parasite of Parupeneus rubescens (Mullidae) from the Arabian Gulf
    Rewaida Abdel-Gaber, Saleh Al Quraishy, Mohamed A. Dkhil, Maysar Abu Hawsah, Lamia Bakr, Sherein Maher
    Journal of King Saud University - Science.2020; 32(3): 2243.     CrossRef
  • Phyllodistomum vaili(Plagiorchiida: Gorgoderidae) infecting Parupeneus rubescens (Perciformes: Mullidae): morphology and phylogeny
    Rewaida Abdel-Gaber, Saleh Al Quraishy, Mohamed Abdel Monem Dkhil, Maysar Abu Hawsah, Masheil Alghamdi, Arwa Althomali, Lamia Bakr, Sherein Maher, Almahy El-Mallah
    Revista Brasileira de Parasitologia Veterinária.2020;[Epub]     CrossRef
  • Genetic diversity of the potentially therapeutic tapeworm Hymenolepis diminuta (Cestoda: Cyclophyllidea)
    Lucie Řežábková, Jan Brabec, Milan Jirků, Marc Dellerba, Roman Kuchta, David Modrý, William Parker, Kateřina Jirků Pomajbíková
    Parasitology International.2019; 71: 121.     CrossRef
  • Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China
    Xiao Xiao, Rui Qi, Hui-Ju Han, Jian-Wei Liu, Xiang-Rong Qin, Li-Zhu Fang, Chuan-Min Zhou, Xiao-Qing Gong, Si-Cong Lei, Xue-Jie Yu
    International Journal for Parasitology: Parasites and Wildlife.2019; 10: 274.     CrossRef
  • A new species of Steringotrema Odhner, 1911 (Trematoda: Fellodistomidae) from the New Zealand sole Peltorhamphus novaezeelandiae Günther off Kaka point in the Catlins, South Island, New Zealand
    Gerardo Pérez-Ponce de León, Thibaut Anglade, Haseeb S. Randhawa
    Systematic Parasitology.2018; 95(2-3): 213.     CrossRef
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    R. E. T. Vanstreels, C. H. Gardiner, M. J. Yabsley, L. Swanepoel, C. K. M. Kolesnikovas, R. P. Silva-Filho, A. C. Ewbank, J. L. Catão-Dias
    Journal of Parasitology.2018; 104(3): 322.     CrossRef
  • Complete mitochondrial genome of the first deep-sea spongicolid shrimp Spongiocaris panglao (Decapoda: Stenopodidea): Novel gene arrangement and the phylogenetic position and origin of Stenopodidea
    Shao'e Sun, Zhongli Sha, Yanrong Wang
    Gene.2018; 676: 123.     CrossRef
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    Microbiome.2018;[Epub]     CrossRef
  • Comparative mitochondrial genomics among Spirometra (Cestoda: Diphyllobothriidae) and the molecular phylogeny of related tapeworms
    Xi Zhang, Jiang Yang Duan, Ya Li Shi, Peng Jiang, De Jun Zeng, Zhong Quan Wang, Jing Cui
    Molecular Phylogenetics and Evolution.2017; 117: 75.     CrossRef
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    Parasitology Research.2016; 115(9): 3329.     CrossRef
  • Sparganosis in wild boar ( Sus scrofa ) – Implications for veterinarians, hunters, and consumers
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    Veterinary Parasitology.2016; 227: 115.     CrossRef
  • The complete mitochondrial genome of the tapeworm Cladotaenia vulturi (Cestoda: Paruterinidae): gene arrangement and phylogenetic relationships with other cestodes
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    Parasites & Vectors.2016;[Epub]     CrossRef
  • The complete mitochondrial genome of Anoplocephala perfoliata, the first representative for the family Anoplocephalidae
    Aijiang Guo
    Parasites & Vectors.2015;[Epub]     CrossRef
  • The first report of sparganosis (Spirometra sp.) in Eurasian badger (Meles meles)
    Marta Kołodziej-Sobocińska, Małgorzata Tokarska, Rafał Kowalczyk
    Parasitology International.2014; 63(2): 397.     CrossRef
  • Multilocus sequence evaluation for differentiating species of the trematode Family Gastrothylacidae, with a note on the utility of mitochondrial COI motifs in species identification
    Sudeep Ghatani, Jollin Andrea Shylla, Bishnupada Roy, Veena Tandon
    Gene.2014; 548(2): 277.     CrossRef
  • Description of two new species of the genus Thaparocleidus Jain, 1952 (Monogenea, Dactylogyridae) from freshwater fish in India: morphological and molecular phylogenetic evidence
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    Journal of Helminthology.2013; 87(2): 160.     CrossRef
  • Molecular characterization of the parasitic tapeworm Bertiella studeri from the island of Mauritius
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    Parasitology Research.2012; 110(2): 759.     CrossRef
  • Secondary structure and phylogenetic utility of the ribosomal large subunit (28S) in monogeneans of the genus Thaparocleidus and Bifurcohaptor (Monogenea: Dactylogyridae)
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    Journal of Parasitic Diseases.2012;[Epub]     CrossRef
  • The life cycle of Asymphylodora perccotti sp. n. (Trematoda: Lissorchiidae) in the Russian Southern Far East
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    Parasitology Research.2011; 108(1): 211.     CrossRef
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Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients
Ying-Hua Xuan, Hak Sun Yu, Hae Jin Jeong, Sung-Yong Seol, Dong-Il Chung, Hyun-Hee Kong
Korean J Parasitol 2007;45(1):1-9.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.1

The endosymbionts of 4 strains of Acanthamoeba (KA/E9, KA/E21, KA/E22, and KA/E23) isolated from the infected corneas of Korean patients were characterized via orcein stain, transmission electron microscopic examination, and 16S rDNA sequence analysis. Double membrane-bound, rod-shaped endosymbionts were distributed randomly throughout both the trophozoites and cysts of each of Acanthamoeba isolates. The endosymbionts of KA/E9, KA/E22, and KA/E23 were surrounded by electron-translucent areas. No lacunae-like structures were observed in the endosymbionts of KA/E21, the bacterial cell walls of which were studded with host ribosomes. Comparative analyses of the 16S rDNA sequences showed that the endosymbionts of KA/E9, KA/E22 and KA/E23 were closely related to Caedibacter caryophilus, whereas the KA/E21 endosymbiont was assigned to the Cytophaga-Flavobacterium-Bacteroides (CFB) phylum. In the 4 strains of Acanthamoeba, the hosts of the endosymbionts were identified as belonging to the Acanthamoeba castellanii complex, which corresponds to the T4 genotype. Acanthamoeba KA/E21 evidenced characteristics almost identical to those of KA/E6, with the exception of the existence of endosymbionts. The discovery of these endosymbionts from Acanthamoeba may prove essential to future studies focusing on interactions between the endosymbionts and the amoebic hosts.

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    Parasitology Research.2024;[Epub]     CrossRef
  • Presence and diversity of free-living amoebae and their potential application as water quality indicators
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    Parasites, Hosts and Diseases.2024; 62(2): 180.     CrossRef
  • Contamination of fresh vegetables in municipal stores with pathogenic Acanthamoeba genotypes; a public health concern
    Marziye Fatemi, Maryam Niyyati, Soheila Rouhani, Seyed Ahmad Karamati, Hamed Mirjalali, Panagiotis Karanis
    International Journal of Environmental Health Research.2023; 33(10): 1010.     CrossRef
  • The life cycle-dependent transcriptional profile of the obligate intracellular amoeba symbiontAmoebophilus asiaticus
    E Selberherr, T Penz, L König, B Conrady, A Siegl, M Horn, S Schmitz-Esser
    FEMS Microbiology Ecology.2022;[Epub]     CrossRef
  • A Systematic Review of Intracellular Microorganisms within Acanthamoeba to Understand Potential Impact for Infection
    Binod Rayamajhee, Dinesh Subedi, Hari Kumar Peguda, Mark Duncan Willcox, Fiona L. Henriquez, Nicole Carnt
    Pathogens.2021; 10(2): 225.     CrossRef
  • Molecular characterization of bacterial, viral and fungal endosymbionts of Acanthamoeba isolates in keratitis patients of Iran
    Elham Hajialilo, Mostafa Rezaeian, Maryam Niyyati, Mohammad Reza Pourmand, Mehdi Mohebali, Mehdi Norouzi, Kobra Razavi Pashabeyg, Sassan Rezaie, Sadegh Khodavaisy
    Experimental Parasitology.2019; 200: 48.     CrossRef
  • Nuclear Group I introns with homing endonuclease genes in Acanthamoeba genotype T4
    Daniele Corsaro, Danielle Venditti
    European Journal of Protistology.2018; 66: 26.     CrossRef
  • Molecular identification of bacterial endosymbionts of Sappinia strains
    Daniele Corsaro, Claudia Wylezich, Julia Walochnik, Danielle Venditti, Rolf Michel
    Parasitology Research.2017; 116(2): 549.     CrossRef
  • Identification of Paenibacillus as a Symbiont in Acanthamoeba
    Vinicius José Maschio, Gertrudes Corção, Francielle Bücker, Karin Caumo, Marilise Brittes Rott
    Current Microbiology.2015; 71(3): 415.     CrossRef
  • Identifying endosymbiont bacteria associated with free‐living amoebae
    Pilar Goñi, María Teresa Fernández, Encarnación Rubio
    Environmental Microbiology.2014; 16(2): 339.     CrossRef
  • Acanthamoeba castellanii cysts: new ultrastructural findings
    Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Anel Lagunes-Guillén, Maritza Omaña-Molina, Martha Espinosa-Cantellano, Adolfo Martínez-Palomo
    Parasitology Research.2013; 112(3): 1125.     CrossRef
  • Functional expression and characterization of an iron-containing superoxide dismutase of Acanthamoeba castellanii
    Jung-Yeon Kim, Byoung-Kuk Na, Kyoung-Ju Song, Mi-Hyun Park, Yun-Kyu Park, Tong-Soo Kim
    Parasitology Research.2012; 111(4): 1673.     CrossRef
  • A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus
    Stephan Schmitz-Esser, Thomas Penz, Anja Spang, Matthias Horn
    BMC Evolutionary Biology.2011;[Epub]     CrossRef
  • Is Acanthamoeba pathogenicity associated with intracellular bacteria?
    Graeme Neil Paterson, Michael Rittig, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
    Experimental Parasitology.2011; 129(2): 207.     CrossRef
  • Detection of Bacterial Endosymbionts in Clinical Acanthamoeba Isolates
    Alfonso Iovieno, Dolena R. Ledee, Darlene Miller, Eduardo C. Alfonso
    Ophthalmology.2010; 117(3): 445.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2010; 29(6): 680.     CrossRef
  • Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality
    Vincent Thomas, Gerald McDonnell, Stephen P. Denyer, Jean-Yves Maillard
    FEMS Microbiology Reviews.2010; 34(3): 231.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2009; 28(3): 348.     CrossRef
  • Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
    Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
    The Korean Journal of Parasitology.2008; 46(3): 157.     CrossRef
  • Diversity of Bacterial Endosymbionts of Environmental Acanthamoeba Isolates
    Stephan Schmitz-Esser, Elena R. Toenshoff, Susanne Haider, Eva Heinz, Verena M. Hoenninger, Michael Wagner, Matthias Horn
    Applied and Environmental Microbiology.2008; 74(18): 5822.     CrossRef
  • 10,065 View
  • 94 Download
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Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear
Sun Joo Lee, Hae Jin Jeong, Ji Eun Lee, Jong Soo Lee, Ying Hua Xuan, Hyun-Hee Kong, Dong-Il Chung, Mee-Sun Ock, Hak Sun Yu
Korean J Parasitol 2006;44(4):313-320.
Published online December 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.4.313

In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.

Citations

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  • Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation
    Da-In Lee, Sung Hee Park, Shin-Ae Kang, Do Hyun Kim, Sun Hyun Kim, So Yeon Song, Sang Eun Lee, Hak Sun Yu
    The Korean Journal of Parasitology.2022; 60(4): 229.     CrossRef
  • Orthokeratology lens-related Acanthamoeba keratitis: case report and analytical review
    Jinfang Wu, Huatao Xie
    Journal of International Medical Research.2021;[Epub]     CrossRef
  • Infectious keratitis and orthokeratology lens use: a systematic review
    Ka Wai Kam, Wing Yung, Gabriel Ka Hin Li, Li Jia Chen, Alvin L. Young
    Infection.2017; 45(6): 727.     CrossRef
  • The Role of Ultraviolet Radiation in the Ocular System of Mammals
    Mercede Majdi, Behrad Milani, Asadolah Movahedan, Lisa Wasielewski, Ali Djalilian
    Photonics.2014; 1(4): 347.     CrossRef
  • Orthokeratology lens related infections
    Kelvin Ho-Nam Wan
    World Journal of Ophthalmology.2014; 4(3): 63.     CrossRef
  • Riboflavin and Ultraviolet Light A Therapy as an Adjuvant Treatment for Medically Refractive Acanthamoeba Keratitis
    Yasin A. Khan, Renata T. Kashiwabuchi, Suy Anne Martins, Juan M. Castro-Combs, Sachin Kalyani, Philip Stanley, David Flikier, Ashley Behrens
    Ophthalmology.2011; 118(2): 324.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2010; 29(6): 680.     CrossRef
  • Twenty Years of Acanthamoeba Keratitis
    F R S Carvalho, A S Foronda, M J Mannis, A L Höfling-Lima, R Belfort, Denise de Freitas
    Cornea.2009; 28(5): 516.     CrossRef
  • Molecular Phylogeny of Acanthamoeba
    Hyun Hee Kong
    The Korean Journal of Parasitology.2009; 47(Suppl): S21.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2009; 28(3): 348.     CrossRef
  • Trends in Microbial Keratitis Associated With Orthokeratology
    Kathleen G. Watt, Helen A. Swarbrick
    Eye & Contact Lens: Science & Clinical Practice.2007; 33(6): 373.     CrossRef
  • 14,068 View
  • 110 Download
  • Crossref

Brief Communications

Molecular cloning of a rhoptry protein (ROP6) secreted from Toxoplasma gondii
Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam
Korean J Parasitol 2006;44(3):251-254.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.251

Monoclonal antibody (mAb) Tg786 against Toxoplasma gondii has been found to detect a 42-kDa rhoptry protein (ROP6) which showed protease activity and host cell binding characteristics after secretion. Using the mAb, a colony containing a 3'-UTR was probed in a T. gondii cDNA expression library. A full length cDNA sequence of the rhoptry protein was completed after 5'-RACE, which consisted of 1,908 bp with a 1,443 bp ORF. The deduced amino acid sequence of ROP6 consisted of a polypeptide of 480 amino acids without significant homology to any other known proteins. This sequence contains an amino terminal stop transfer sequence downstream of a short neutral sequence, hydrophilic middle sequence, and hydrophobic carboxy terminus. It is suggested that the ROP6 is inserted into the rhoptry membrane with both N- and C-termini.

Citations

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  • A systematic review of Toxoplasma gondii antigens to find the best vaccine candidates for immunization
    Fatemeh Rezaei, Shahabeddin Sarvi, Mahdi Sharif, Seyed Hossein Hejazi, Abdol sattar Pagheh, Sargis A. Aghayan, Ahmad Daryani
    Microbial Pathogenesis.2019; 126: 172.     CrossRef
  • Toxoplasma gondii Secretory Proteins and their Role in Invasion and Pathogenesis
    Yang Zhang, Bo Shiun Lai, Mario Juhas, Yun Zhang
    Microbiological Research.2019;[Epub]     CrossRef
  • A systematic review on the role of GRA proteins of Toxoplasma gondii in host immunization
    Fatemeh Rezaei, Mahdi Sharif, Shahabeddin Sarvi, Seyed Hossein Hejazi, Sargis Aghayan, Abdol Sattar Pagheh, Samira Dodangeh, Ahmad Daryani
    Journal of Microbiological Methods.2019; 165: 105696.     CrossRef
  • Rhoptry protein 6 from Toxoplasma gondii is an intrinsically disordered protein
    Won-Kyu Lee, Hye-Jin Ahn, Yeon Gyu Yu, Ho-Woo Nam
    Protein Expression and Purification.2014; 101: 146.     CrossRef
  • Electrophoretic Patterns of Toxoplasma gondii Excreted/Secreted Antigens and Their Role in Induction of the Humoral Immune Response
    Ahmad Daryani, Mehdi Sharif, Hamed Kalani, Alireza Rafiei, Farzad Kalani, Ehsan Ahmadpour
    Jundishapur Journal of Microbiology.2014;[Epub]     CrossRef
  • Kiss and spit: the dual roles of Toxoplasma rhoptries
    John C. Boothroyd, Jean-Francois Dubremetz
    Nature Reviews Microbiology.2008; 6(1): 79.     CrossRef
  • Characterization of a New Gene <italic>wx2</italic> in <italic>Toxoplasma gondii</italic>
    Xiang Wu, Qiong Zhang, Kui Tan, Ronghua Xie, Jiubo Fan, Hengping Shu, Shiping Wang
    Acta Biochimica et Biophysica Sinica.2007; 39(7): 475.     CrossRef
  • 7,234 View
  • 52 Download
  • Crossref
Cloning of the novel putative apoptosis-related gene of Spirometra erinacei (Order Pseudophyllidea)
Soo-Ung Lee, Sun Huh
Korean J Parasitol 2006;44(3):233-237.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.233

We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.

Citations

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  • The genome of the sparganosis tapeworm Spirometra erinaceieuropaeiisolated from the biopsy of a migrating brain lesion
    Hayley M Bennett, Hoi Ping Mok, Effrossyni Gkrania-Klotsas, Isheng J Tsai, Eleanor J Stanley, Nagui M Antoun, Avril Coghlan, Bhavana Harsha, Alessandra Traini, Diogo M Ribeiro, Sascha Steinbiss, Sebastian B Lucas, Kieren SJ Allinson, Stephen J Price, Thom
    Genome Biology.2014;[Epub]     CrossRef
  • 8,218 View
  • 56 Download
  • Crossref
Original Articles
Genetic diversity of Acanthamoeba isolates from ocean sediments
Hua Liu, Young-Ran Ha, Sung-Tae Lee, Yean-Chul Hong, Hyun-Hee Kong, Dong-Il Chung
Korean J Parasitol 2006;44(2):117-125.
Published online June 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.2.117

Genetic diversity of 18 Acanthamoeba isolates from ocean sediments was evaluated by comparing mitochondrial (mt) DNA RFLP, 18S rDNA sequences and by examining their cytopathic effects on human corneal epithelial cells versus reference strains. All isolates belonged to morphologic group II. Total of 16 restriction phenotypes of mtDNA from 18 isolates demonstrated the genetic diversity of Acanthamoeba in ocean sediments. Phylogenetic analysis using 18s rDNA sequences revealed that the 18 isolates were distinct from morphological groups I and III. Fifteen isolates showed close relatedness with 17 clinical isolates and A. castellanii Castellani and formed a lineage equivalent to T4 genotype of Byers' group. Two reference strains from ocean sediment, A. hatchetti BH-2 and A. griffini S-7 clustered unequivocally with these 15 isolates. Diversity among isolates was also evident from their cytopathic effects on human corneal cells. This is the first time describing Acanthamoeba diversity in ocean sediments in Korea.

Citations

Citations to this article as recorded by  Crossref logo
  • Learning from the rDNA Operon: A Reanalysis of the Acanthamoeba palestinensis Group
    Daniele Corsaro
    Microorganisms.2024; 12(10): 2105.     CrossRef
  • The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections
    Paul A. Fuerst
    Microorganisms.2023; 11(2): 295.     CrossRef
  • Detection of potentially pathogenic free-living amoebae from the Caspian Sea and hospital ward dust of teaching hospitals in Guilan, Iran
    Mohammad Reza Mahmoudi, Nozhat Zebardast, Frederick R. Masangkay, Panagiotis Karanis
    Journal of Water and Health.2021; 19(2): 278.     CrossRef
  • Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)
    Stas Malavin, Lyubov Shmakova
    European Journal of Protistology.2020; 73: 125671.     CrossRef
  • Isolation and identification of free-living amoeba from the hot springs and beaches of the Caspian Sea
    Alireza Latifi, Mahboobeh Salami, Elham Kazemirad, Mohammad Soleimani
    Parasite Epidemiology and Control.2020; 10: e00151.     CrossRef
  • Update on Acanthamoeba phylogeny
    Daniele Corsaro
    Parasitology Research.2020; 119(10): 3327.     CrossRef
  • Nuclear Group I introns with homing endonuclease genes in Acanthamoeba genotype T4
    Daniele Corsaro, Danielle Venditti
    European Journal of Protistology.2018; 66: 26.     CrossRef
  • Molecular detection of Acanthamoeba spp., Naegleria fowleri and Vermamoeba (Hartmannella) vermiformis as vectors for Legionella spp. in untreated and solar pasteurized harvested rainwater
    Penelope H. Dobrowsky, Sehaam Khan, Thomas E. Cloete, Wesaal Khan
    Parasites & Vectors.2016;[Epub]     CrossRef
  • Metagenomic characterization of viral communities in Goseong Bay, Korea
    Jinik Hwang, So Yun Park, Mirye Park, Sukchan Lee, Yeonhwa Jo, Won Kyong Cho, Taek-Kyun Lee
    Ocean Science Journal.2016; 51(4): 599.     CrossRef
  • Pandoraviruses: Amoeba Viruses with Genomes Up to 2.5 Mb Reaching That of Parasitic Eukaryotes
    Nadège Philippe, Matthieu Legendre, Gabriel Doutre, Yohann Couté, Olivier Poirot, Magali Lescot, Defne Arslan, Virginie Seltzer, Lionel Bertaux, Christophe Bruley, Jérome Garin, Jean-Michel Claverie, Chantal Abergel
    Science.2013; 341(6143): 281.     CrossRef
  • Mycobacterium gilvum Illustrates Size-Correlated Relationships between Mycobacteria and Acanthamoeba polyphaga
    Otmane Lamrabet, Michel Drancourt
    Applied and Environmental Microbiology.2013; 79(5): 1606.     CrossRef
  • Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida)
    Daniele Corsaro, Danielle Venditti
    Parasitology Research.2010; 107(1): 233.     CrossRef
  • Barcoding Amoebae: Comparison of SSU, ITS and COI Genes as Tools for Molecular Identification of Naked Lobose Amoebae
    Elena Nassonova, Alexey Smirnov, Jose Fahrni, Jan Pawlowski
    Protist.2010; 161(1): 102.     CrossRef
  • Molecular Phylogeny of Acanthamoeba
    Hyun Hee Kong
    The Korean Journal of Parasitology.2009; 47(Suppl): S21.     CrossRef
  • Biodiversity of amoebae and amoebae‐resisting bacteria in a drinking water treatment plant
    Vincent Thomas, Jean‐François Loret, Michel Jousset, Gilbert Greub
    Environmental Microbiology.2008; 10(10): 2728.     CrossRef
  • Relationship between mycobacteria and amoebae: ecological and epidemiological concerns
    V. Thomas, G. McDonnell
    Letters in Applied Microbiology.2007; 45(4): 349.     CrossRef
  • 9,620 View
  • 70 Download
  • Crossref
Expressed sequence tags analysis of Blattella germanica
Hyang Suk Chung, Tai Hyun Yu, Bong Jin Kim, Sun Mi Kim, Joo Yeong Kim, Hak Sun Yu, Hae Jin Jeong, Mee Sun Ock
Korean J Parasitol 2005;43(4):149-156.
Published online December 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.4.149

Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.

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  • The Distribution Characteristics of Microsatellites in the Transcriptome of Periplaneta americana
    Lifang Zhang, Shangeng He
    Cytology and Genetics.2023; 57(5): 491.     CrossRef
  • Identification and characterization of microRNAs in American cockroach (Periplaneta americana)
    Qiao Yang, Zheng Bao, Mingyu Yang, Yongmei Shen, Xiuyue Zhang, Bisong Yue, Yang Meng, Zhenxin Fan
    Gene.2020; 743: 144610.     CrossRef
  • New insight into foregut functions of xenobiotic detoxification in the cockroach Periplaneta americana
    Jian‐Hua Zhang, Song Zhang, Yuan‐Xue Yang, Yi‐Xi Zhang, Ze‐Wen Liu
    Insect Science.2018; 25(6): 978.     CrossRef
  • Cockroach allergen serine proteinases: Isolation, sequencing and signalling via proteinase‐activated receptor‐2
    D. J. Polley, K. Mihara, R. Ramachandran, H. Vliagoftis, B. Renaux, M. Saifeddine, M. O. Daines, S. Boitano, M. D. Hollenberg
    Clinical & Experimental Allergy.2017; 47(7): 946.     CrossRef
  • De Novo Transcriptome Analysis and Detection of Antimicrobial Peptides of the American Cockroach Periplaneta americana (Linnaeus)
    In-Woo Kim, Joon Ha Lee, Sathiyamoorthy Subramaniyam, Eun-Young Yun, Iksoo Kim, Junhyung Park, Jae Sam Hwang, Surajit Bhattacharjya
    PLOS ONE.2016; 11(5): e0155304.     CrossRef
  • Midgut Transcriptome of the Cockroach Periplaneta americana and Its Microbiota: Digestion, Detoxification and Oxidative Stress Response
    Jianhua Zhang, Yixi Zhang, Jingjing Li, Meiling Liu, Zewen Liu, Xinghui Qiu
    PLOS ONE.2016; 11(5): e0155254.     CrossRef
  • Analysis of German cockroach (Blattella germanica) expressed sequence tags
    Ji Eun Jeong, Hee Ju Hwang, Hong Seog Park, Hee Jae Cha, Yong Seok Lee, Meesun Ock
    Genes & Genomics.2015; 37(3): 271.     CrossRef
  • Allergenic Characterization of a Novel Allergen, Homologous to Chymotrypsin, from German Cockroach
    Kyoung Yong Jeong, Mina Son, Jae-Hyun Lee, Chein-Soo Hong, Jung-Won Park
    Allergy, Asthma & Immunology Research.2015; 7(3): 283.     CrossRef
  • De novo Assembly and Characterization of the Testis Transcriptome and Development of EST-SSR Markers in the Cockroach Periplaneta americana
    Wan Chen, Yu-Xiang Liu, Guo-Fang Jiang
    Scientific Reports.2015;[Epub]     CrossRef
  • De Novo Transcriptome of the Hemimetabolous German Cockroach (Blattella germanica)
    Xiaojie Zhou, Kun Qian, Ying Tong, Junwei Jerry Zhu, Xinghui Qiu, Xiaopeng Zeng, Yara M. Traub-Csekö
    PLoS ONE.2014; 9(9): e106932.     CrossRef
  • Identification of differentially expressed genes in American cockroach ovaries and testes by suppression subtractive hybridization and the prediction of its miRNAs
    Wan Chen, Guo-Fang Jiang, Shu-Hong Sun, Yong Lu, Fei Ma, Bin Li
    Molecular Genetics and Genomics.2013; 288(11): 627.     CrossRef
  • 9,620 View
  • 78 Download
  • Crossref
Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses
Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung
Korean J Parasitol 2005;43(1):7-13.
Published online March 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.1.7

The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet to be assessed. In this paper, we analyzed the morphological characteristics, nuclear 18s rDNA and mitochondrial 16s rDNA sequences and the Mt DNA RFLP of the type strains of four Acanthamoeba species, which had been previously designated as A. divionensis, A. parasidionensis, A. mauritaniensis, and A. rhysodes. The four isolates revealed characteristic group II morphology. They exhibited 18S rDNA sequence differences of 0.2-1.1% with each other, but more than 2% difference from the other compared reference strains. Four isolates formed a different clade from that of A. castellanii Castellani and the other strains in morphological group II on the phylogenetic tree. In light of these results, A. paradivionensis, A. divionensis, and A. mauritaniensis should be regarded as synonyms for A. rhysodes.

Citations

Citations to this article as recorded by  Crossref logo
  • The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections
    Paul A. Fuerst
    Microorganisms.2023; 11(2): 295.     CrossRef
  • On the diversity and clinical importance of Acanthamoeba spp. from Group 1
    Daniele Corsaro
    Parasitology Research.2021; 120(6): 2057.     CrossRef
  • Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)
    Stas Malavin, Lyubov Shmakova
    European Journal of Protistology.2020; 73: 125671.     CrossRef
  • Update on Acanthamoeba phylogeny
    Daniele Corsaro
    Parasitology Research.2020; 119(10): 3327.     CrossRef
  • Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
    Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
    The Korean Journal of Parasitology.2013; 51(4): 401.     CrossRef
  • Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia
    Li-Li Chan, Joon-Wah Mak, Yoon-Tong Low, Thuan-Tzen Koh, Init Ithoi, Shar Mariam Mohamed
    Acta Tropica.2011; 117(1): 23.     CrossRef
  • Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
    Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
    The Korean Journal of Parasitology.2008; 46(3): 157.     CrossRef
  • Acanthamoeba keratitis due to Acanthamoeba genotype T4 in a non-contact-lens wearer in Turkey
    Hatice Ertabaklar, Meral Türk, Volkan Dayanir, Sema Ertuğ, Julia Walochnik
    Parasitology Research.2007; 100(2): 241.     CrossRef
  • 8,783 View
  • 78 Download
  • Crossref
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