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"Han-Jong Rim"

Original Article

Effectiveness of Mass Drug Administration on Neglected Tropical Diseases in Schoolchildren in Zanzibar, Tanzania
Ju Yeong Kim, Seobo Sim, Eun Joo Chung, Han-Jong Rim, Jong-Yil Chai, Duk-Young Min, Keeseon S. Eom, Khalfan A. Mohammed, Iddi S. Khamis, Tai-Soon Yong
Korean J Parasitol 2020;58(2):109-119.
Published online April 30, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.2.109
Soil-transmitted helminths and Schistosoma haematobium affect more than 3 billion people globally and mainly occur in sub-Saharan Africa. The present study assessed the overall infection status of a 1716-student cohort of school-children in Zanzibar and applied mass drug administration (MDA) to the cohort from 2007 to 2009. Schools in Pemba, Zanzibar, had a much higher prevalence of soil-transmitted helminth infections than those in Unguja, and the Chaani, Ghana, and Machui schools of Unguja exhibited high S. haematobium infection rates. The MDA program only partially controlled parasite infections, owing to high rates of re-infection. The infection rate of S. haematobium across all 10 schools, for example, was only reduced by 1.8%, and even this change not significant, even though the S. haematobiuminfection rates of the Chaani and Mzambarauni schools were significantly reduced from 64.4 and 23.4%, respectively, at the first screening, to 7.3 and 2.3% at the last screening. The overall infection rate of Ascaris lumbricoides was reduced from 36.0% at the first screening to 22.6% at the last screening. However, the infection rates for both Trichuris trichiuraand hookworm were generally unaffected by MDA. In the future, parasite control programs should involve strategically designed MDA schedules and holistic intervention (e.g., sanitation improvement, hygiene behavior changes, and control of intermediated hosts).

Citations

Citations to this article as recorded by  Crossref logo
  • Effects of paediatric schistosomiasis control programmes in sub-Saharan Africa: A systematic review
    Maryline Vere, Wilma ten Ham-Baloyi, Paula Ezinne Melariri, Raquel Inocencio da Luz
    PLOS ONE.2024; 19(5): e0301464.     CrossRef
  • Soil-transmitted helminth (STH) infections in the Wolaita zone in Southern Ethiopia: mid-stage evaluation of the Geshiyaro project and progress towards the interruption of transmission
    Birhan Mengistu, Ewnetu Firdawek Liyew, Melkie Chernet, Geremew Tasew, Rosie Maddren, Benjamin Collyer, Ufaysa Anjulo, Adugna Tamiru, Kathryn Forbes, Zelalem Mehari, Kebede Deribe, Teshale Yadeta, Mihretab Salasibew, Getachew Tollera, Roy Anderson
    Parasites & Vectors.2024;[Epub]     CrossRef
  • Parasitic helminth infections in humans modulate Trefoil Factor levels in a manner dependent on the species of parasite and age of the host
    Babatunde Adewale, Jonathan R. Heintz, Christopher F. Pastore, Heather L. Rossi, Li-Yin Hung, Nurudeen Rahman, Jeff Bethony, David Diemert, James Ayorinde Babatunde, De’Broski R. Herbert, Subash Babu
    PLOS Neglected Tropical Diseases.2021; 15(10): e0009550.     CrossRef
  • 7,562 View
  • 181 Download
  • 4 Web of Science
  • Crossref

Brief Communications

Echinostoma aegyptica (Trematoda: Echinostomatidae) Infection in Five Riparian People in Savannakhet Province, Lao PDR
Jong-Yil Chai, Bong-Kwang Jung, Taehee Chang, Hyejoo Shin, Woon-Mok Sohn, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim
Korean J Parasitol 2020;58(1):67-72.
Published online February 29, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.1.67
Human infection with Echinostoma aegyptica Khalil and Abaza, 1924 (Trematoda: Echinostomatidae) is extremely rare. In this study, we confirmed E. aegyptica infection in 5 riparian residents living along the Mekong River in Savannakhet Province, Lao PDR. The patients revealed eggs of Opisthorchis viverrini/minute intestinal flukes, echinostomes, and other parasites in fecal examinations using the Kato-Katz technique. Following treatment with praziquantel 30-40 mg/kg and pyrantel pamoate 10-15 mg/kg in a single dose and purging with magnesium salts, adult specimens of various helminth species were collected. Among the trematodes, echinostome flukes of 4.5-7.6 mm in length (n = 134; av. 22.3 specimens per case) were of taxonomic interest and subjected in this study. The flukes were morphologically characterized by having total 43-45 collar spines arranged in 2 alternating rows (corner spines usually 5 on each side) and compatible with previous descriptions of E. aegyptica. The patients were mixed-infected with other helminths, so specific clinical manifestations due to this echinostome fluke were difficult to determine. The present paper describes for the first time human E. aegyptica infections in Lao PDR. This is the second report of human infection (2nd-6th cases) with E. aegyptica in the world following the first one from China.

Citations

Citations to this article as recorded by  Crossref logo
  • High endemicity of Opisthorchis viverrini infection among people in northern Cambodia confirmed by adult worm expulsion
    Bong-Kwang Jung, Sooji Hong, Taehee Chang, Jaeeun Cho, Seungwan Ryoo, Keon Hoon Lee, Jeonggyu Lee, Woon-Mok Sohn, Sung-Jong Hong, Virak Khieu, Rekol Huy, Jong-Yil Chai
    Scientific Reports.2023;[Epub]     CrossRef
  • Neglected food-borne trematodiases: echinostomiasis and gastrodiscoidiasis
    Rafael Toledo, María Álvarez-Izquierdo, J. Guillermo Esteban, Carla Muñoz-Antoli
    Parasitology.2022; 149(10): 1319.     CrossRef
  • Echinostoma mekongi n. sp. (Digenea: Echinostomatidae) from Riparian People along the Mekong River in Cambodia
    Jaeeun Cho, Bong-Kwang Jung, Taehee Chang, Woon-Mok Sohn, Muth Sinuon, Jong-Yil Chai
    The Korean Journal of Parasitology.2020; 58(4): 431.     CrossRef
  • 8,078 View
  • 121 Download
  • 5 Web of Science
  • Crossref
Echinochasmus caninus n. comb. (Trematoda: Echinostomatidae) Infection in Eleven Riparian People in Khammouane Province, Lao PDR
Jong-Yil Chai, Taehee Chang, Bong-Kwang Jung, Hyejoo Shin, Woon-Mok Sohn, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim
Korean J Parasitol 2019;57(4):451-456.
Published online August 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.4.451
Adult specimens of Echinochasmus caninus n. comb. (Verma, 1935) (Trematoda: Echinostomatidae) (syn. Episthmium caninum Yamaguti, 1958) were recovered from 11 riparian people who resided along the Mekong River in Khammouane Province, Lao PDR. In fecal examinations done by the Kato-Katz technique, the cases revealed eggs of Opisthorchis viverrini/minute intestinal flukes, hookworms, and in 2 cases echinostome eggs. To recover the adult helminths, praziquantel 30-40 mg/kg and pyrantel pamoate 10-15 mg/kg in a single dose were given and purged with magnesium salts. Various species of trematodes (including O. viverrini and Haplorchis spp.), cestodes, and nematodes were recovered from their diarrheic stools. Among the trematodes, small echinostome flukes (n=42; av. 3.8 specimens per case) of 0.7-1.2 mm in length are subjected in this study. They are morphologically characterized by having 24 collar spines interrupted dorsally and anterior extension of vitellaria from the cirrus sac or genital pore level to the posterior end of the body. Particularly based on this extensive distribution of vitellaria, the specific diagnosis was made as Echinochasmus caninus. The cases were co-infected with various other helminth parasites; thus, clinical manifestations specific for this echinostome infection were difficult to determine. The present paper describes for the first time human E. caninus infections in Lao PDR. Our cases marked the 4-14th human infections with this echinostome around the world following the 3 previous cases reported from Thailand.

Citations

Citations to this article as recorded by  Crossref logo
  • Neglected food-borne trematodiases: echinostomiasis and gastrodiscoidiasis
    Rafael Toledo, María Álvarez-Izquierdo, J. Guillermo Esteban, Carla Muñoz-Antoli
    Parasitology.2022; 149(10): 1319.     CrossRef
  • General overview of the current status of human foodborne trematodiasis
    Jong-Yil Chai, Bong-Kwang Jung
    Parasitology.2022; 149(10): 1262.     CrossRef
  • Echinostoma aegyptica (Trematoda: Echinostomatidae) Infection in Five Riparian People in Savannakhet Province, Lao PDR
    Jong-Yil Chai, Bong-Kwang Jung, Taehee Chang, Hyejoo Shin, Woon-Mok Sohn, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim
    The Korean Journal of Parasitology.2020; 58(1): 67.     CrossRef
  • Echinostoma mekongi n. sp. (Digenea: Echinostomatidae) from Riparian People along the Mekong River in Cambodia
    Jaeeun Cho, Bong-Kwang Jung, Taehee Chang, Woon-Mok Sohn, Muth Sinuon, Jong-Yil Chai
    The Korean Journal of Parasitology.2020; 58(4): 431.     CrossRef
  • Echinostoma macrorchis Metacercariae in Cipangopaludina chinensis malleata from Xiengkhuang Province, Lao PDR and Morphologies of Adults from Experimental Animals
    Woon-Mok Sohn, Byoung-Kuk Na, Dongmin Lee, Keeseon S. Eom, Tai-Soon Yong, Jong-Yil Chai, Duk-Young Min
    The Korean Journal of Parasitology.2019; 57(6): 657.     CrossRef
  • 8,206 View
  • 155 Download
  • 7 Web of Science
  • Crossref
Echinostoma ilocanum Infection in Two Residents of Savannakhet Province, Lao PDR
Jong-Yil Chai, Woon-Mok Sohn, Jaeeun Cho, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Han-Jong Rim
Korean J Parasitol 2018;56(1):75-79.
Published online February 28, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.1.75
Adult specimens of Echinostoma ilocanum (Garrison, 1908) Odhner, 1911 (Trematoda: Echinostomatidae) were recovered from 2 riparian people who resided along the Mekong River in Savannakhet Province, Lao PDR. In fecal examinations done by the Kato-Katz technique, they revealed echinostome eggs together with eggs of Opisthorchis viverrini (and minute intestinal fluke eggs) and hookworms. To recover the adult flukes, they were treated with praziquantel 30-40 mg/kg in a single dose and purged with magnesium salts. A total of 658 adult fluke specimens were recovered from the 2 people; 456 from case 1 and 202 from case 2. Specimens from case 1 consisted of 335 echinostomes (301 E. ilocanum and 34 species undetermined), 120 O. viverrini, and 1 Haplorchis taichui, and those from case 2 consisted of 36 E. ilocanum, 134 O. viverrini, and 32 H. taichui. Thus, the number of E. ilocanum specimens was 337 in total (average per person, 168.5). From this study, it is suggested that foodborne intestinal flukes and liver flukes are highly prevalent along the Mekong River in Savannakhet Province. The present report describes for the first time human infections with E. ilocanum in Lao PDR.

Citations

Citations to this article as recorded by  Crossref logo
  • Development and utilization of a visual loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) assay for rapid detection of Echinostomatidae metacercaria in edible snail samples
    Wasin Panich, Phonkawin Jaruboonyakorn, Awika Raksaman, Thanawan Tejangkura, Thapana Chontananarth
    International Journal of Food Microbiology.2024; 418: 110732.     CrossRef
  • Neglected food-borne trematodiases: echinostomiasis and gastrodiscoidiasis
    Rafael Toledo, María Álvarez-Izquierdo, J. Guillermo Esteban, Carla Muñoz-Antoli
    Parasitology.2022; 149(10): 1319.     CrossRef
  • General overview of the current status of human foodborne trematodiasis
    Jong-Yil Chai, Bong-Kwang Jung
    Parasitology.2022; 149(10): 1262.     CrossRef
  • Foodborne intestinal flukes: A brief review of epidemiology and geographical distribution
    Jong-Yil Chai, Bong-Kwang Jung
    Acta Tropica.2020; 201: 105210.     CrossRef
  • Echinostoma aegyptica (Trematoda: Echinostomatidae) Infection in Five Riparian People in Savannakhet Province, Lao PDR
    Jong-Yil Chai, Bong-Kwang Jung, Taehee Chang, Hyejoo Shin, Woon-Mok Sohn, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim
    The Korean Journal of Parasitology.2020; 58(1): 67.     CrossRef
  • Echinostoma mekongi n. sp. (Digenea: Echinostomatidae) from Riparian People along the Mekong River in Cambodia
    Jaeeun Cho, Bong-Kwang Jung, Taehee Chang, Woon-Mok Sohn, Muth Sinuon, Jong-Yil Chai
    The Korean Journal of Parasitology.2020; 58(4): 431.     CrossRef
  • Echinochasmus caninus n. comb. (Trematoda: Echinostomatidae) Infection in Eleven Riparian People in Khammouane Province, Lao PDR
    Jong-Yil Chai, Taehee Chang, Bong-Kwang Jung, Hyejoo Shin, Woon-Mok Sohn, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim
    The Korean Journal of Parasitology.2019; 57(4): 451.     CrossRef
  • 10,231 View
  • 177 Download
  • 11 Web of Science
  • Crossref

Articles from Symposium on Controls of NTDs around Lake Victoria, Tanzania

High Malaria Prevalence among Schoolchildren on Kome Island, Tanzania
Min-Jae Kim, Bong-Kwang Jung, Jong-Yil Chai, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Julius E. Siza, Godfrey M. Kaatano, Josephat Kuboza, Peter Mnyeshi, John M. Changalucha, Yunsuk Ko, Su Young Chang, Han-Jong Rim
Korean J Parasitol 2015;53(5):571-574.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.571
In order to determine the status of malaria among schoolchildren on Kome Island (Lake Victoria), near Mwanza, Tanzania, a total of 244 schoolchildren in 10 primary schools were subjected to a blood survey using the fingerprick method. The subjected schoolchildren were 123 boys and 121 girls who were 6-8 years of age. Only 1 blood smear was prepared for each child. The overall prevalence of malaria was 38.1% (93 positives), and sex difference was not remarkable. However, the positive rate was the highest in Izindabo Primary School (51.4%) followed by Isenyi Primary School (48.3%) and Bugoro Primary School (46.7%). The lowest prevalence was found in Muungano Primary School (16.7%) and Nyamiswi Primary School (16.7%). These differences were highly correlated with the location of the school on the Island; those located in the peripheral area revealed higher prevalences while those located in the central area showed lower prevalences. Plasmodium falciparum was the predominant species (38.1%; 93/244), with a small proportion of them mixed-infected with Plasmodium vivax (1.6%; 4/244). The results revealed that malaria is highly prevalent among primary schoolchildren on Kome Island, Tanzania, and there is an urgent need to control malaria in this area.

Citations

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  • Malaria among schoolchildren in urban, peri-urban and rural settings in Eastern Ethiopia: repeated cross section study
    Hailu Merga, Teshome Degefa, Zewdie Birhanu, Ephrem Abiy, Ming-Chieh Lee, Guiyun Yan, Delenasaw Yewhalaw
    Malaria Journal.2025;[Epub]     CrossRef
  • Malaria Species Positivity Rates Among Symptomatic Individuals Across Regions of Differing Transmission Intensities in Mainland Tanzania
    Zachary R Popkin-Hall, Misago D Seth, Rashid A Madebe, Rule Budodo, Catherine Bakari, Filbert Francis, Dativa Pereus, David J Giesbrecht, Celine I Mandara, Daniel Mbwambo, Sijenunu Aaron, Abdallah Lusasi, Samwel Lazaro, Jeffrey A Bailey, Jonathan J Julian
    The Journal of Infectious Diseases.2024; 229(4): 959.     CrossRef
  • High prevalence and risk of malaria among asymptomatic individuals from villages with high prevalence of artemisinin partial resistance in Kyerwa district of Kagera region, north-western Tanzania
    Salehe S. Mandai, Filbert Francis, Daniel P. Challe, Misago D. Seth, Rashid A. Madebe, Daniel A. Petro, Rule Budodo, Angelina J. Kisambale, Gervas A. Chacha, Ramadhan Moshi, Ruth B. Mbwambo, Dativa Pereus, Catherine Bakari, Sijenunu Aaron, Daniel Mbwambo,
    Malaria Journal.2024;[Epub]     CrossRef
  • Similar Prevalence of Plasmodium falciparum and Non–P. falciparum Malaria Infections among Schoolchildren, Tanzania1
    Rachel Sendor, Cedar L. Mitchell, Frank Chacky, Ally Mohamed, Lwidiko E. Mhamilawa, Fabrizio Molteni, Ssanyu Nyinondi, Bilali Kabula, Humphrey Mkali, Erik J. Reaves, Naomi Serbantez, Chonge Kitojo, Twilumba Makene, Thwai Kyaw, Meredith Muller, Alexis Mwan
    Emerging Infectious Diseases.2023;[Epub]     CrossRef
  • Performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for Plasmodium falciparum malaria in North-West Tanzania
    Alphaxard Manjurano, Justin J. Omolo, Eric Lyimo, Donald Miyaye, Coleman Kishamawe, Lucas E. Matemba, Julius J. Massaga, John Changalucha, Paul E. Kazyoba
    Malaria Journal.2021;[Epub]     CrossRef
  • Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards
    Philip Koliopoulos, Neema Mathias Kayange, Tim Daniel, Florian Huth, Britta Gröndahl, Grey Carolina Medina-Montaño, Leah Pretsch, Julia Klüber, Christian Schmidt, Antke Züchner, Sebastian Ulbert, Steven E. Mshana, Marylyn Addo, Stephan Gehring
    Malaria Journal.2021;[Epub]     CrossRef
  • Prevalence of asymptomatic malaria, submicroscopic parasitaemia and anaemia in Korogwe District, north-eastern Tanzania
    Paul M. Hayuma, Christian W. Wang, Edwin Liheluka, Vito Baraka, Rashid A. Madebe, Daniel T. R. Minja, Gerald Misinzo, Michael Alifrangis, John P. A. Lusingu
    Malaria Journal.2021;[Epub]     CrossRef
  • Malaria Morbidities Following Universal Coverage Campaign for Long-Lasting Insecticidal Nets: A Case Study in Ukerewe District, Northwestern Tanzania


    Anthony Kapesa, Namanya Basinda, Elias C. Nyanza, Joshua Monge, Sospatro E Ngallaba, Joseph R Mwanga, Eliningaya J Kweka
    Research and Reports in Tropical Medicine.2020; Volume 11: 53.     CrossRef
  • Uncomplicated falciparum malaria among schoolchildren in Bajil district of Hodeidah governorate, west of Yemen: association with anaemia and underweight
    Talal S. Alwajeeh, Rashad Abdul-Ghani, Amal F. Allam, Hoda F. Farag, Safia S. M. Khalil, Amel Y. Shehab, Mona H. El-Sayad, Raed A. Alharbi, Shaia S. R. Almalki, Ahmed A. Azazy
    Malaria Journal.2020;[Epub]     CrossRef
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    S. Peprah, H. Dhudha, H. Ally, N. Masalu, E. Kawira, C. N. Chao, I. O. Genga, M. Mumia, P. A. Were, T. Kinyera, I. Otim, I. D. Legason, R. J. Biggar, K. Bhatia, J. J. Goedert, R. M. Pfeiffer, S. M. Mbulaiteye
    Tropical Medicine & International Health.2019; 24(5): 571.     CrossRef
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    Amir Alelign, Zinaye Tekeste, Beyene Petros
    BMC Public Health.2018;[Epub]     CrossRef
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    Hugo Kavunga-Membo, Gillon Ilombe, Justin Masumu, Junior Matangila, Joël Imponge, Emile Manzambi, Francis Wastenga, Dieudonné Mumba Ngoyi, Jean-Pierre Van Geetruyden, Jean Jacques Muyembe
    Malaria Journal.2018;[Epub]     CrossRef
  • Indoor residual spraying with micro-encapsulated pirimiphos-methyl (Actellic® 300CS) against malaria vectors in the Lake Victoria basin, Tanzania
    Fabian M. Mashauri, Alphaxard Manjurano, Safari Kinung’hi, Jackline Martine, Eric Lyimo, Coleman Kishamawe, Chacha Ndege, Mahdi M. Ramsan, Adeline Chan, Charles D. Mwalimu, John Changalucha, Stephen Magesa, Clive Shiff
    PLOS ONE.2017; 12(5): e0176982.     CrossRef
  • 11,193 View
  • 128 Download
  • 13 Web of Science
  • Crossref
Improved Perceptions and Practices Related to Schistosomiasis and Intestinal Worm Infections Following PHAST Intervention on Kome Island, North-Western Tanzania
Joseph R. Mwanga, Godfrey M. Kaatano, Julius E. Siza, Su Young Chang, Yunsuk Ko, Cyril M. Kullaya, Jackson Nsabo, Keeseon S. Eom, Tai-Soon Yong, Jong-Yil Chai, Duk-Young Min, Han-Jong Rim, John M. Changalucha
Korean J Parasitol 2015;53(5):561-569.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.561
Schistosomiasis and intestinal worm infections are widespread diseases of public health importance in Tanzania. A study on perceptions and practices related to schistosomiasis and intestinal worm infections was undertaken among a community population of Kome Island in Sengerema District, north-western Tanzania, where intestinal schistosomiasis and intestinal worm infections are endemic. Schistosomiasis and intestinal worm-related perceptions and practices were assessed before and 3 years after implementation of a participatory hygiene and sanitation transformation (PHAST) intervention as a control measure. Data were obtained from baseline and post-intervention knowledge, attitudes, and practices (KAP) questionnaire surveys conducted twice in 2009 and 2012 among 82 individuals aged ≥15 years. We found significant increases in respondents’ knowledge of the cause, transmission, symptoms, health consequences, and prevention of schistosomiasis and intestinal worm infections after PHAST intervention. The increase in respondents’ knowledge on almost all aspects of the said infections was translated into actions to control schistosomiasis and intestinal worm infections. This has not been achieved by chance, but due to well-designed and locally-adapted PHAST intervention. We conclude that despite criticisms, PHAST approach is still useful in empowering communities to control water, sanitation, and hygiene related infectious diseases such as schistosomiasis and intestinal worm infections.

Citations

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  • Effects of an integrated intervention on schistosomiasis prevalence in a rural area of Tanzania
    Yoonho Cho, Jungim Lee, Humphrey Deogratias Mazigo, Leah Elisha Salamba, Seungman Cha, Sutas Suttiprapa
    PLOS Neglected Tropical Diseases.2025; 19(7): e0013215.     CrossRef
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    Masunga Simon Tryphone, Kassim Moh’d Ali, Edward Joseph Macha
    European Journal of Theoretical and Applied Sciences.2024; 2(5): 676.     CrossRef
  • Contextual factors influencing schistosomiasis treatment and identification of delivery platforms for arpraziquantel in hard-to-reach areas and populations in Homa Bay County, Kenya
    Phyllis Munyiva Isaiah, Doris Osei Afriyie, Mary Maghanga, Donna Obare Ogeto, Mary Amuyunzu Nyamongo, Peter Steinmann, Julia Robinson
    PLOS Global Public Health.2024; 4(12): e0004035.     CrossRef
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    Valeria Silvestri, Mwanahawa Idavas Mshana, Vivian Mushi, Witness M. Bonaventura, Nyanda C. Justine, Clemence Kinabo, Abdallah Zacharia, Giuseppe La Torre, Billy Ngasala
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    Carlos A. Torres-Vitolas, Suzan C. M. Trienekens, Willemijn Zaadnoordijk, Anouk N. Gouvras, Alberto Novaes Ramos
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  • Interventions to improve water, sanitation, and hygiene for preventing soil-transmitted helminth infection
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  • Molecular Detection of Haplorchis pumilio Eggs in Schoolchildren, Kome Island, Lake Victoria, Tanzania
    Hyejoo Shin, Bong-Kwang Jung, Seungwan Ryoo, Sooji Hong, Heonwoo Jeong, Hoo-Gn Jeoung, Sunhye Kim, Sun Kim, Min-Jae Kim, Hansol Park, Keeseon S. Eom, Godfrey M. Kaatano, Jong-Yil Chai
    Emerging Infectious Diseases.2022; 28(11): 2298.     CrossRef
  • Blood Flukes and Arterial Damage: A Review of Aneurysm Cases in Patients with Schistosomiasis
    Valeria Silvestri, Vivian Mushi, Mwanahawa Idavas Mshana, Witness M. Bonaventura, Nyanda C. Justine, Deodatus Sabas, Billy Ngasala, Arif Siddiqui
    Canadian Journal of Infectious Diseases and Medical Microbiology.2022; 2022: 1.     CrossRef
  • Water, sanitation, and hygiene for schistosomiasis prevention: a qualitative analysis of experiences of stakeholders in rural KwaZulu-Natal
    Chanelle Mulopo, Moses J. Chimbari
    Journal of Water, Sanitation and Hygiene for Development.2021; 11(2): 255.     CrossRef
  • From the One Health Perspective: Schistosomiasis Japonica and Flooding
    Su-Ying Guo, Lu Li, Li-Juan Zhang, Yin-Long Li, Shi-Zhu Li, Jing Xu
    Pathogens.2021; 10(12): 1538.     CrossRef
  • Village Response to Mass Drug Administration for Schistosomiasis in Mwanza Region, Northwestern Tanzania: Are We Missing Socioeconomic, Cultural, and Political Dimensions?
    Joseph R. Mwanga, Safari M. Kinung’hi, Justina Mosha, Teckla Angelo, Jane Maganga, Carl H. Campbell
    The American Journal of Tropical Medicine and Hygiene.2020; 103(5): 1969.     CrossRef
  • Parasitic infections in relation to practices and knowledge in a rural village in Northern Thailand with emphasis on fish-borne trematode infection
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    Epidemiology and Infection.2019;[Epub]     CrossRef
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    Campbell Systematic Reviews.2017; 13(1): 1.     CrossRef
  • Integrated Schistosomiasis and Soil-Transmitted Helminthiasis Control over Five Years on Kome Island, Tanzania
    Godfrey M. Kaatano, Julius E. Siza, Joseph R. Mwanga, Duk-Yong Min, Tai-Soon Yong, Jong-Yil Chai, Yunsuk Ko, Su Young Chang, Cyril M. Kullaya, Han-Jong Rim, John M. Changalucha, Keeseon S. Eom
    The Korean Journal of Parasitology.2015; 53(5): 535.     CrossRef
  • 11,717 View
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Improved Socio-Economic Status of a Community Population Following Schistosomiasis and Intestinal Worm Control Interventions on Kome Island, North-Western Tanzania
Joseph R. Mwanga, Godfrey M. Kaatano, Julius E. Siza, Su Young Chang, Yunsuk Ko, Cyril M. Kullaya, Jackson Nsabo, Keeseon S. Eom, Tai-Soon Yong, Jong-Yil Chai, Duk-Young Min, Han-Jong Rim, John M. Changalucha
Korean J Parasitol 2015;53(5):553-559.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.553
Research on micro-level assessment of the changes of socio-economic status following health interventions is very scarce. The use of household asset data to determine wealth indices is a common procedure for estimating socio-economic position in resource poor settings. In such settings information about income is usually lacking, and the collection of individual consumption or expenditure data would require in-depth interviews, posing a considerable risk of bias. In this study, we determined the socio-economic status of 213 households in a community population in an island in the north-western Tanzania before and 3 year after implementation of a participatory hygiene and sanitation transformation (PHAST) intervention to control schistosomiasis and intestinal worm infections. We constructed a household 'wealth index' based housing construction features (e.g., type of roof, walls, and floor) and durable assets ownership (e.g., bicycle, radio, etc.). We employed principal components analysis and classified households into wealth quintiles. The study revealed that asset variables with positive factor scores were associated with higher socio-economic status, whereas asset variables with negative factor scores were associated with lower socio-economic status. Overall, households which were rated as the poorest and very poor were on the decrease, whereas those rated as poor, less poor, and the least poor were on the increase after PHAST intervention. This decrease/increase was significant. The median shifted from -0.4376677 to 0.5001073, and the mean from -0.2605787 (SD; 2.005688) to 0.2605787 (SD; 1.831199). The difference in socio-economic status of the people between the 2 phases was highly statistically significant (P<0.001). We argue that finding of this study should be treated with caution as there were other interventions to control schistosomiasis and intestinal worm infections which were running concurrently on Kome Island apart from PHAST intervention.

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  • Socioeconomic determinants of Schistosoma mansoni infection using multiple correspondence analysis among rural western Kenyan communities: Evidence from a household-based study
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Schistosoma mansoni-Related Hepatosplenic Morbidity in Adult Population on Kome Island, Sengerema District, Tanzania
Godfrey M. Kaatano, Duk-Young Min, Julius E. Siza, Tai-Soon Yong, Jong-Yil Chai, Yunsuk Ko, Su-Young Chang, John M. Changalucha, Keeseon S. Eom, Han-Jong Rim
Korean J Parasitol 2015;53(5):545-551.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.545
Schistosomiasis is one of the important neglected tropical diseases (NTDs) in Tanzania, particularly in Lake Victoria zone. This baseline survey was a part of the main study of integrated control of schistosomiasis and soil-transmitted helminths (STHs) aimed at describing morbidity patterns due to intestinal schistosomiasis among adults living on Kome Island, Sengerema District, Tanzania. Total 388 adults from Kome Islands (about 50 people from each village) aged between 12 and 85 years, were examined by abdominal ultrasound according to the Niamey protocol. Liver image patterns (LIPs) A and B were considered normal, and C-F as distinct periportal fibrosis (PPF). The overall prevalence of PPF was 42.2%; much higher in males than in females (47.0% in male vs 34.4% in females, P=0.007). Abnormal increase of segmental branch wall thickness (SBWT) and dilated portal vein diameter (PVD) were also more common in males than in females. Hepatosplenomegaly was frequently encountered; 68.1% had left liver lobe hepatomegaly and 55.2% had splenomegaly. Schistosoma mansoni-related morbidity is quite high among adults in this community justifying the implementation of integrated control strategies through mass drug administration, improved water supply (pumped wells), and health education that had already started in the study area.

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    Daniel W. Gunda, Elizabeth F. Mtui, Paulina M. Manyiri, David C. Majinge, Semvua B. Kilonzo, Humphrey D. Mazigo, Benson R. Kidenya
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    Hannah J Russell, James M StJ Penney, Cortland Linder, Elizabeth C Joekes, Amaya L Bustinduy, J Russell Stothard, Daniel A L Rakotomampianina, Emmanuel H Andriamasy, Lalarizo R Mahary, Elodie P Ranjanoro, Alain M Rahetilahy, Stephen A Spencer
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    Helena A. Ngowi
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  • Intestinal schistosomiasis of Ijinga Island, north-western Tanzania: prevalence, intensity of infection, hepatosplenic morbidities and their associated factors
    Andreas Mueller, Antje Fuss, Uwe Ziegler, Godfrey M. Kaatano, Humphrey D. Mazigo
    BMC Infectious Diseases.2019;[Epub]     CrossRef
  • Integrating use of point-of-care circulating cathodic antigen rapid diagnostic tests by community health workers during mass drug administration campaigns to improve uptake of praziquantel treatment among the adult population at Kome Island, North-Western
    Humphrey D. Mazigo, John H. Amuasi, Isaac Osei, Safari M. Kinung’hi
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  • Association Between Polymorphisms of the Mannose-Binding Lectin and Severity of Periportal Fibrosis in Schistosomiasis, in the Northeast of Brazil
    Taynan da Silva Constantino, Elker Lene Santos de Lima, Lidiane Régia Pereira Braga de Brito, Jamile Luciana Silva, Maria Rosângela Cunha Duarte Coêlho, Maria Tereza Cartaxo Muniz, Paula Carolina Valença Silva, Ana Lúcia Coutinho Domingues
    Genetic Testing and Molecular Biomarkers.2017; 21(9): 571.     CrossRef
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    Humphrey D. Mazigo, Fred Nuwaha, David W. Dunne, Godfrey M. Kaatano, Tekla Angelo, Stella Kepha, Safari M. Kinung’hi
    The Korean Journal of Parasitology.2017; 55(5): 533.     CrossRef
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  • Is ultrasonography useful for population studies on schistosomiasis mansoni? An evaluation based on a survey on a population from Kome Island, Tanzania
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  • 11,593 View
  • 124 Download
  • 18 Web of Science
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Integrated Schistosomiasis and Soil-Transmitted Helminthiasis Control over Five Years on Kome Island, Tanzania
Godfrey M. Kaatano, Julius E. Siza, Joseph R. Mwanga, Duk-Yong Min, Tai-Soon Yong, Jong-Yil Chai, Yunsuk Ko, Su Young Chang, Cyril M. Kullaya, Han-Jong Rim, John M. Changalucha, Keeseon S. Eom
Korean J Parasitol 2015;53(5):535-543.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.535
Integrated control strategies are important for sustainable control of schistosomiasis and soil-transmitted helminthiasis, despite their challenges for their effective implementation. With the support of Good Neighbors International in collaboration with National Institute of Medical Research, Mwanza, Tanzania, integrated control applying mass drug administration (MDA), health education using PHAST, and improved safe water supply has been implemented on Kome Island over 5 years for controlling schistosomiasis and soil-transmitted helminths (STHs). Baseline surveys for schistosomiasis and STHs was conducted before implementation of any integrated control strategies, followed by 4 cross-sectional follow-up surveys on randomly selected samples of schoolchildren and adults in 10 primary schools and 8 villages, respectively, on Kome islands. Those follow-up surveys were conducted for impact evaluation after introduction of control strategies interventions in the study area. Five rounds of MDA have been implemented from 2009 along with PHAST and improved water supply with pumped wells as other control strategies for complementing MDA. A remarkable steady decline of schistosomiasis and STHs was observed from 2009 to 2012 with significant trends in their prevalence decline, and thereafter infection rate has remained at a low sustainable control. By the third follow-up survey in 2012, Schistosoma mansoni infection prevalence was reduced by 90.5% and hookworm by 93.3% among schoolchildren while in adults the corresponding reduction was 83.2% and 56.9%, respectively. Integrated control strategies have successfully reduced S. mansoni and STH infection status to a lower level. This study further suggests that monitoring and evaluation is a crucial component of any large-scale STH and schistosomiasis intervention.

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    Yoonho Cho, Jungim Lee, Humphrey Deogratias Mazigo, Leah Elisha Salamba, Seungman Cha, Sutas Suttiprapa
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    Gemechu Tadesse, Yonas Wuletaw, Kalkidan Mekete, Heven Sime, Elodie Yard, Laura Appleby, Jack Grimes, Nigussie Dejene, Iain Gardiner, Adama Kazienga, Souheila Abbeddou, Michael French, Bruno Levecke, Lesley Drake
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    Humphrey D. Mazigo, Antonio Montresor
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    Franco Zacharia, Valeria Silvestri, Vivian Mushi, George Ogweno, Twilumba Makene, Lwidiko E. Mhamilawa, David Zadock Munisi
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    Tereza Cristina Favre, Cristiano Lara Massara, Lilian Christina N?brega Holsbach Beck, Roc?o Karina Saavedra Acero Cabello, Otavio Sarmento Pieri
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  • Factors associated with relevant knowledge of intestinal schistosomiasis and intention to participate in treatment campaigns: a cross sectional survey among school children at Ijinga Island on Lake Victoria, North-Western Tanzania
    Sandra Parisi, Humphrey D. Mazigo, Saskia Kreibich, Karl Puchner, Christa Kasang, Andreas Mueller
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Prevalence of Schistosomes and Soil-Transmitted Helminths and Morbidity Associated with Schistosomiasis among Adult Population in Lake Victoria Basin, Tanzania
Julius E. Siza, Godfrey M. Kaatano, Jong-Yil Chai, Keeseon S. Eom, Han-Jong Rim, Tai-Soon Yong, Duk-Young Min, Su Young Chang, Yunsuk Ko, John M. Changalucha
Korean J Parasitol 2015;53(5):525-533.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.525
The
objective
of this study was to carry out a community survey on schistosomiais and soil-transmitted helminth (STH) infections in order to suggest feasible and effective intervention strategies in Lake Victoria basin, Tanzania. A total of 37 communities selected from 23 districts of the 4 regions in the Lake Victoria basin of Tanzania were involved in the study. From each of the selected locality, 50 adult community members, 25 males and 25 females, were recruited for the study. Each study participant was requested to submit stool and urine specimens. From each stool specimen, duplicate Kato-Katz thick smears were prepared and microscopically examined for Schistosoma mansoni and STH eggs. Urine specimens were processed by the filtration technique and microscopically examined for Schistosoma haematobium eggs. Ultrasound examination for morbidity due to schistosomiasis was performed. Mass treatment was done using praziquantel and albendazole for schistosome and STHs infections, respectively. Out of 1,606 adults who provided stool specimens, 199 (12.4%) were positive for S. mansoni, 349 (21.7%) for hookworms, 133 (8.3%) for Ascaris lumbricoides, and 33 (2.0%) for Trichuris trichiura. Out of 1,400 participants who provided urine specimens, 25 (1.8%) were positive for S. haematobium eggs. Because of the co-endemicity of these afflictions and their impact on vulnerable population groups, the helminthiasis could be simultaneously treated with 2 drugs, praziquantel for schistosomiasis and albendazole for STHs.

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Prevalence of Schistosomes and Soil-Transmitted Helminths among Schoolchildren in Lake Victoria Basin, Tanzania
Julius E. Siza, Godfrey M. Kaatano, Jong-Yil Chai, Keeseon S. Eom, Han-Jong Rim, Tai-Soon Yong, Duk-Young Min, Su Young Chang, Yunsuk Ko, John M. Changalucha
Korean J Parasitol 2015;53(5):515-524.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.515
The
objective
s of this study was to conduct a survey on schistosomiasis and soil-transmitted helminth (STH) infections in order to come up with feasible control strategies in Lake Victoria basin, Tanzania. Depending on the size of the school, 150-200 schoolchildren were recruited for the study. Duplicate Kato-Katz stool smears were prepared from each child and microscopically examined for Schistosoma mansoni and STHs. Urine specimens were examined for Schistosoma haematobium eggs using the filtration technique. After the survey, mass drug administration was done using praziquantel and albendazole for schistosomiasis and STHs infections, respectively. A total of 5,952 schoolchildren from 36 schools were recruited for the study and had their stool and urine specimens examined. Out of 5,952 schoolchildren, 898 (15.1%) were positive for S. mansoni, 754 (12.6%) for hookworms, 188 (3.2%) for Ascaris lumblicoides, and 5 (0.008%) for Trichuris trichiura. Out of 5,826 schoolchildren who provided urine samples, 519 (8.9%) were positive for S. haematobium eggs. The results revealed that intestinal schistosomiasis, urogenital schistosomiasis, and STH infections are highly prevalent throughought the lake basin. The high prevalence of intestinal and urogenital schistosomisiasis in the study area was a function of the distance from Lake Victoria, the former being more prevalent at localities close to the lake, whilst the latter is more so away from it. Control of schistosomiasis and STHs in the study area requires an integrated strategy that involves provision of health education to communities, regular treatments, and provision of adequate safe water supply and sanitation facilities.

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Original Articles

Infection Status of Zoonotic Trematode Metacercariae in Fishes from Vientiane Municipality and Champasak Province in Lao PDR
Keeseon S. Eom, Han-Sol Park, Dongmin Lee, Woon-Mok Sohn, Tai-Soon Yong, Jong-Yil Chai, Duk-Young Min, Han-Jong Rim, Bounnaloth Insisiengmay, Bounlay Phommasack
Korean J Parasitol 2015;53(4):447-453.
Published online August 25, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.4.447
The infection status of fishborne zoonotic trematode (FZT) metacercariae was investigated in fishes from 2 localities of Lao PDR. Total 157 freshwater fishes (17 species) were collected in local markets of Vientiane Municipality and Champasak Province in December 2010 and July 2011, and each fish was examined by the artificial digestion method. Total 6 species of FZT metacercariae, i.e., Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, Haplorchis pumilio, Centrocestus formosanus, and Procerovum varium, were detected in fishes from Vientiane Municipality. The metacercariae of O. viverrini were detected in 50 (49.5%) out of 101 fishes (6 species), and their average number was 154 per fish infected. The remaining 5 species of heterophyid metacercariae were detected in 36.8%, 65.8%, 9.4%, 23.9%, and 5.1% fishes examined, and their average densities were 12, 1,038, 4, 15, and 13 per infected fish, respectively. In fishes from Champasak Province, 3 species of FZT metacercariae, i.e., O. viverrini, H. taichui, and H. yokogawai, were detected. Only 2 O. viverrini metacercariae were found in only 1 Barbonymus schwanefeldi. The metacercariae of H. taichui and H. yokogawai were detected in 60.0% and 50.0% of fishes examined, and their average densities were 47 and 28 per fish infected. By the present study, it has been confirmed that several species of FZT metacercariae are prevalent in fishes from Vientiane Municipality, with P. varium being a new member of FZT in Lao PDR. In comparison, FZT metacercariae are less prevalent in fishes from Champasak Province.

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Intestinal Helminths Recovered from Humans in Xieng Khouang Province, Lao PDR with a Particular Note on Haplorchis pumilio Infection
Jong-Yil Chai, Woon-Mok Sohn, Bong-Kwang Jung, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Bounnaloth Insisiengmay, Sithat Insisiengmay, Bounlay Phommasack, Han-Jong Rim
Korean J Parasitol 2015;53(4):439-445.
Published online August 25, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.4.439
A survey of intestinal helminths was undertaken in riparian people in Xieng Khouang Province, Lao PDR. Fecal specimens were collected from 643 people (289 males and 354 females) residing in 4 districts (Nonghet, Kham, Phoukout, and Pek) and were examined by the Kato-Katz technique. The overall helminth egg positive rate was 41.2%, and hookworms revealed the highest prevalence (32.7%) followed by Trichuris trichiura (7.3%) and Ascaris lumbricoides (5.6%). The positive rate for small trematode eggs (STE), which may include Opisthorchis viverrini, heterophyids, and lecithodendriids, was 4.4%. For recovery of adult helminths, 12 STE or nematode/cestode egg-positive people were treated with 40 mg/kg praziquantel and 15 mg/kg pyrantel pamoate, and then purged. Mixed infections with 2 Haplorchis species (H. pumilio and H. taichui), Centrocestus formosanus, Opisthorchis viverrini, a species of cestode (Taenia saginata), and several species of nematodes including hookworms and Enterobius vermicularis were detected. The worm load for trematodes was the highest for H. pumilio with an average of 283.5 specimens per infected person followed by C. formosanus, H. taichui, and O. viverrini. The worm load for nematodes was the highest for hookworms (21.5/infected case) followed by E. vermicularis (3.2/infected case). The results revealed that the surveyed areas of Xieng Khouang Province, Lao PDR are endemic areas of various species of intestinal helminths. The STE found in the surveyed population were verified to be those of heterophyids, particularly H. pumilio.

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Prevalence of Helminthic Infections among Inhabitants of Lao PDR
Keeseon S. Eom, Tai-Soon Yong, Woon-Mok Sohn, Jong-Yil Chai, Duk-Young Min, Han-Jong Rim, Hyeong-Kyu Jeon, Virasack Banouvong, Bounnaloth Insisiengmay, Bounlay Phommasack
Korean J Parasitol 2014;52(1):51-56.
Published online February 19, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.1.51

The prevalence of helminthic infections was surveyed on a total of 6,178 residents (males 2,549 and females 3,629) in 102 villages of 9 provinces in Lao PDR between 2007 and 2011 under the project of Korea-Laos Collaborative Project for Control of Foodborne Trematode Infections in Lao PDR. Fecal specimens were collected and examined by the Kato-Katz thick smear and Stoll's egg counting techniques. The overall liver/intestinal helminth egg positive rate was 71.9% with a single or mixed infections with Opisthorchis viverrini and minute intestinal flukes (Ov/MIF), Ascaris lumbricoides, hookworms, Trichuris trichiura, Trichostrongylus sp., echinostomes, Taenia spp., and others. Ov/MIF revealed the highest prevalence (55.6%) followed by hookworms (27.8%) and T. trichiura (6.5%). The endemic regions with the highest prevalence of Ov/MIF were Savannakhet, Khammouane, Vientiane (Nam Ngum), Champasak (Khong Island), and Saravane Province. High prevalences of A. lumbricoides (33.8%), hookworms (47.8%), and T. trichiura (32.6%) were observed in Phongsaly, Luang Prabang, and Vientiane (Nam Ngum) areas, respectively. The results of this study highlight helminth parasites of current public health significance in different areas of Lao PDR.

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Complete Mitochondrial Genome of Haplorchis taichui and Comparative Analysis with Other Trematodes
Dongmin Lee, Seongjun Choe, Hansol Park, Hyeong-Kyu Jeon, Jong-Yil Chai, Woon-Mok Sohn, Tai-Soon Yong, Duk-Young Min, Han-Jong Rim, Keeseon S. Eom
Korean J Parasitol 2013;51(6):719-726.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.719

Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.

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Brief Communication

Human Neurocysticercosis Case and an Endemic Focus of Taenia solium in Lao PDR
Hyeong-Kyu Jeon, Tai-Soon Yong, Woon-Mok Sohn, Jong-Yil Chai, Duk-Young Min, Han-Jong Rim, Bounnaloth Insisiengmay, Keeseon S. Eom
Korean J Parasitol 2013;51(5):599-602.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.599

A male patient with neurocysticercosis was identified in Montai Village, Xay District, Oudomxay Province, Lao PDR in February 2004. He had a history of diagnosis for neurocysticercosis by a CT scan in Thailand after an onset of epileptic seizure in 1993. A pig in the same district was found to contain Taenia solium metacestodes (=cysticerci); the slaughtered pig body contained more than 2,000 cysticerci. In addition to morphological identification, molecular identification was also performed on the cysticerci by DNA sequencing analysis of the mitochondrial cox1 gene; they were confirmed as T. solium metacestodes. The patient is regarded as an indigenous case of neurocysticercosis infected in an endemic focus of T. solium taeniasis/cysticercosis in Oudomxay Province, Lao PDR.

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    Andrew Larkins, Mieghan Bruce, Amanda Ash
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    Andrew Larkins, Mieghan Bruce, Rattanxay Phetsouvanh, Amanda Ash
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Original Article

Hyperendemicity of Haplorchis taichui Infection among Riparian People in Saravane and Champasak Province, Lao PDR
Jong-Yil Chai, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Hyeong-Kyu Jeon, Tae-Yun Kim, Bong-Kwang Jung, Lay Sisabath, Bounnaloth Insisiengmay, Bounlay Phommasack, Han-Jong Rim
Korean J Parasitol 2013;51(3):305-311.
Published online June 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.3.305

In this study, we found that Haplorchis taichui, a heterophyid intestinal fluke, is highly prevalent, with heavy worm loads, among riparian people in Saravane and Champasak province, Lao PDR. Fecal specimens were collected from 1,460 people (717 men and 743 women) in 12 riparian (Mekong river) districts and were examined by the Kato-Katz fecal smear technique. The overall helminth egg positive rate was 78.8% and 66.4% in Saravane and Champasak province, respectively. The positive rate for small trematode eggs (STE), which included H. taichui and other heterophyids, Opisthorchis viverrini, and lecithodendriids, was 69.9% and 46.3% in Saravane and Champasak province, respectively. To obtain adult flukes, 30 STE-positive people were treated with 40 mg/kg praziquantel and then purged. Whole diarrheic stools were collected 4-5 times for each person and searched for fluke specimens using a stereomicroscope. Mixed infections with various species of trematodes (H. taichui, Haplorchis pumilio, O. viverrini, Prosthodendrium molenkampi, Centrocestus formosanus, and Echinochasmus japonicus) and a species of cestode (Taenia saginata) were found. However, the worm load was exceptionally high for H. taichui compared with other trematode species, with an average of 21,565 and 12,079 specimens per infected person in Saravane and Champasak province, respectively, followed by H. pumilio (41.9 and 22.5, respectively) and O. viverrini (9.4 and 1.5, respectively). These results show that diverse species of intestinal and liver flukes are prevalent among riparian people in Saravane and Champasak province, Lao PDR, with H. taichui being the exceptionally dominant species.

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Brief Communication

Current Status of Human Taeniasis in Lao People's Democratic Republic
Hyeong-Kyu Jeon, Tai-Soon Yong, Woon-Mok Sohn, Jong-Yil Chai, Duk-Young Min, Cheong-Ha Yun, Han-Jong Rim, Tiengkham Pongvongsa, Virasack Banouvong, Bounnaloth Insisiengmay, Bounlay Phommasack, Keeseon S. Eom
Korean J Parasitol 2013;51(2):259-263.
Published online April 25, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.2.259

Human taeniasis was investigated in Lao People's Democratic Republic (Lao PDR) between 2000 and 2011 as part of the nation's helminthiasis survey. A total of 55,038 inhabitants, including 29,846 school children, were examined using the Kato-Katz and scotch-tape anal swab method, and morphological observation of adult worms. Molecular identification of Taenia tapeworms was performed by multiplex PCR or DNA sequence analysis of the mitochondrial cox1 gene. Taenia eggs were present at a rate of 1.5% (845/55,038) in the subject population. Adult tapeworms were identified as T. solium or T. saginata by analyzing the collectable stool specimens (n=126). Three specimens identified as T. solium were found in Luang Prabang, while the remaining 123 specimens, which were T. saginata, were found in Bokeo, Bolikhamxay, Champasak, Houaphan, Khammouane, Luang Namta, Luang Prabang, Oudomxay, Phongsaly, Saysomboune, Saravane, Savannakhet, Xayaboury, Xekong, Xieng Khouang Province, and Vientiane Municipality.

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Original Articles

Echinostoma macrorchis in Lao PDR: Metacercariae in Cipangopaludina Snails and Adults from Experimentally Infected Animals
Woon-Mok Sohn, Jong-Yil Chai, Byoung-Kuk Na, Tai-Soon Yong, Keeseon S. Eom, Hansol Park, Duk-Young Min, Han-Jong Rim
Korean J Parasitol 2013;51(2):191-196.
Published online April 25, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.2.191

The echinostome metacercariae encysted in Cipangopaludina sp. snails that were purchased from a market in Vientiane Municipality, Lao PDR, were identified as Echinostoma macrorchis (Digenea: Echinostomatidae) through recovery of adult flukes after experimental infection to rats and a cat. The metacercariae were round, 113-128 (121)×113-125 (120) ?m, having a thick cyst wall, a head collar armed with collar spines, and excretory granules. The adult flukes recovered from the rats and cat at day 14 and 30 post-infection, respectively, were elongated, ventrally curved, and 3.9-6.3×0.7-1.1 mm in size. The head collar was distinct, bearing 43-45 collar spines with 5 angle spines on each side. Two testes were large (as the name implies), tandem, and slightly constricted at the middle, with irregular margins. Eggs were operculated, ovoid to elliptical, and 88-95×56-60 ?m. In scanning electron microscopy, the head collar was prominent, with 43-45 collar spines. Scale-like tegumental spines were densely distributed on the ventral surface between the oral and ventral suckers. Sensory papillae were distributed mainly on the tegument around the 2 suckers. It is confirmed that E. macrorchis is distributed in Lao PDR using Cipangopaludina sp. snails as the second intermediate host.

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Fishborne Trematode Metacercariae in Luang Prabang, Khammouane, and Saravane Province, Lao PDR
Han-Jong Rim, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Jong-Yil Chai, Duk-Young Min, Soon-Hyung Lee, Eui-Hyug Hoang, Bounlay Phommasack, Sithat Insisiengmay
Korean J Parasitol 2013;51(1):107-114.
Published online February 18, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.1.107

Fishborne trematode (FBT) metacercariae were investigated in fish from 3 Provinces of Lao PDR. Total 242 freshwater fish of 40 species were collected in local markets of Luang Prabang (59 fish of 16 species), Khammouane (81 fish of 19 species), and Saravane (97 fish of 14 species), and each of them was examined by artificial digestion method. Four species of metacercariae (Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, and Centrocestus formosanus) were detected. O. viverrini was detected in 35 fish (14.5%), and their density was 252 per infected fish (Luang Prabang, 88 metacercariae in 5 fish; Khammouane, 187 in 6 fish; Saravane, 303 in 24 fish). H. taichui was found in 102 fish (42.1%), and their density was 485 per infected fish (Luang Prabang, 260 metacercariae in 38 fish; Khammouane, 1,084 in 23 fish; Saravane, 359 in 41 fish). H. yokogawai was detected in 92 fish (38.0%), and their density was 222 per infected fish (Luang Prabang, 362 metacercariae in 17 fish; Khammouane, 126 in 20 fish; Saravane, 214 in 55 fish). Metacercariae of C. formosanus were found in 8 fish (3.3%), and their density was 3 per infected fish. In the present study, it has been confirmed that FBT metacercariae, in particular, H. taichui, H. yokogawai, and O. viverrini, are highly prevalent in fish from Luang Prabang, Khammouane, and Saravane Province, Lao PDR.

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Brief Communications

Human Infections with Liver and Minute Intestinal Flukes in Guangxi, China: Analysis by DNA Sequencing, Ultrasonography, and Immunoaffinity Chromatography
Hyeong-Kyu Jeon, Dongmin Lee, Hansol Park, Duk-Young Min, Han-Jong Rim, Hongman Zhang, Yichao Yang, Xueming Li, Keeseon S Eom
Korean J Parasitol 2012;50(4):391-394.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.391

The prevalence of liver and intestinal fluke infections was determined by surveying inhabitants of Hengxuan, Fusui, and Shanglin villages which were known to be endemic for liver flukes in Guangxi, China in May 2010. A total of 718 people were examined for helminth eggs by the Kato-Katz thick smear technique, ultrasonography, immunoaffinity chromatography, and DNA sequencing. The overall egg positive rate was found to be 59.6% (28.0-70.6%) that included mixed infections with liver and intestinal flukes. Cases showing higher than 20,000 eggs per gram of feces (EPG) were detected between 1.3% and 16.2%. Ultrasonographic findings exhibited overall 28.2% (72 of 255 cases) dilatation rate of the intrahepatic bile duct. Clonorchis sinensis infection was detected serologically in 88.3% (38 of 43 cases) among C. sinensis egg positive subjects by the immunoaffinity chromatography using a specific antigen for C. sinensis. For differential diagnosis of the liver and intestinal flukes, more precise PCR and nucleotide sequencing for copro-DNA were performed for 46 egg positive cases. Mixed infections with C. sinensis and Metagonimus yokogawai were detected in 8 of 46 egg positive cases, whereas 29 specimens were positive for Haplorchis taichui. Ultrasonographic findings and immunoaffinity chromatography results showed usefulness, even in a limited way, in figuring out of the liver fluke endemicity.

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Echinostome Flukes Receovered from Humans in Khammouane Province, Lao PDR
Jong-Yil Chai, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Eui-Hyug Hoang, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim
Korean J Parasitol 2012;50(3):269-272.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.269

Echinostome eggs were detected in 22 (1.1%) of 2,074 people residing in riparian villages along the Mekong River in Khammouane Province, Lao PDR. In order to recover the adult flukes, 9 persons were treated with praziquantel and purged with magnesium salts. A total of 55 echinostome specimens (4 species) were recovered from the 9 persons. Echinostoma revolutum (8 specimens) was recovered from 3 persons, Artyfechinostomum malayanum (8 specimens) was from 2 persons, Echinochasmus japonicus (33 specimens) was from 7 persons, and Euparyphium sp. (6 specimens) was from 1 person. In Lao PDR, only human infections with E. japonicus were previously known. Therefore, the present study describes human infections with E. revolutum, A. malayanum, and Euparyphium sp. for the first time in Lao PDR. These results indicate that the surveyed villages of Khammouane Province, Lao PDR are low-grade endemic foci of echinostomiasis.

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Original Article

Morphologic and Genetic Identification of Taenia Tapeworms in Tanzania and DNA Genotyping of Taenia solium
Keeseon S. Eom, Jong-Yil Chai, Tai-Soon Yong, Duk-Young Min, Han-Jong Rim, Charles Kihamia, Hyeong-Kyu Jeon
Korean J Parasitol 2011;49(4):399-403.
Published online December 16, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.4.399

Species identification of Taenia tapeworms was performed using morphologic observations and multiplex PCR and DNA sequencing of the mitochondrial cox1 gene. In 2008 and 2009, a total of 1,057 fecal samples were collected from residents of Kongwa district of Dodoma region, Tanzania, and examined microscopically for helminth eggs and proglottids. Of these, 4 Taenia egg positive cases were identified, and the eggs were subjected to DNA analysis. Several proglottids of Taenia solium were recovered from 1 of the 4 cases. This established that the species were T. solium (n=1) and T. saginata (n=3). One further T. solium specimen was found among 128 fecal samples collected from Mbulu district in Arusha, and this had an intact strobila with the scolex. Phylegenetic analysis of the mtDNA cox1 gene sequences of these 5 isolates showed that T. saginata was basal to the T. solium clade. The mitochondrial cox1 gene sequences of 3 of these Tanzanian isolates showed 99% similarity to T. saginata, and the other 2 isolates showed 100% similarity to T. solium. The present study has shown that Taenia tapeworms are endemic in Kongwa district of Tanzania, as well as in a previously identified Mbulu district. Both T. solium isolates were found to have an "African/Latin American" genotype (cox1).

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Brief Communication

Prevalence of the Intestinal Flukes Haplorchis taichui and H. yokogawai in a Mountainous Area of Phongsaly Province, Lao PDR
Jong-Yil Chai, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Eun-Hee Shin, Virasack Banouvong, Bounnaloth Insisiengmay, Sithat Insisiengmay, Bounlay Phommasack, Han-Jong Rim
Korean J Parasitol 2010;48(4):339-342.
Published online December 16, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.4.339

Phongsaly Province, located in the northernmost area of Lao PDR, was previously suggested to be endemic for the liver fluke Opisthorchis viverrini infection. To confirm, or rule out, this suggestion, the Phonxay village in the Khoua District, Phongsaly Province, was selected for a survey. Ten volunteers (8 men and 2 women aged 31-57 years) who consumed raw freshwater fish and had gastrointestinal troubles were treated with a single dose of praziquantel (40 mg/kg) and pyrantel pamoate (10 mg/kg) and purged with magnesium sulfate to recover any worm parasites. Eight of the 10 volunteers expelled 1 or more species of trematodes, nematodes, or cestodes (worm positive rate; 80%). The worms were morphologically identified as H. taichui (861 worms from 8 people), H. yokogawai (59 from 6 people), Phaneropsolus bonnei (1 from 1 person), Trichostrongylus sp. (2 from 2 people), Ascaris lumbricoides (2 from 1 person), Enterobius vermicularis (11 from 3 people), and Taenia saginata (1 strobila with scolex from 1 person). The results indicate that the mountainous area of Phongsaly Province, Lao PDR, is not endemic for the liver fluke but endemic for intestinal flukes, in particular, Haplorchis taichui and H. yokogawai.

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Original Article

Morphologic and Genetic Identification of Diphyllobothrium nihonkaiense in Korea
Hyeong-Kyu Jeon, Kyu-Heon Kim, Sun Huh, Jong-Yil Chai, Duk-Young Min, Han-Jong Rim, Keeseon S. Eom
Korean J Parasitol 2009;47(4):369-375.
Published online December 1, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.4.369

Diphyllobothrium nihonkaiense was first described by Yamane in 1986 but the taxonomical features have been obscure due to lack of critical morphologic criteria in its larval and adult stages. In Korea, this tapeworm had long been known as Diphyllobothrium latum. In this study, we observed 62 specimens collected from Korean residents and analyzed them by morphological features and nucleotide sequences of mitochondrial cox1 gene as well as the ITS1 region. Adult tapeworms were examined after carmine or trichrome stain. Longitudinal sections of the gravid proglottids showed an obtuse angle of about 150 degree between the cirrus sac and seminal vesicle. This angle is known as a major differential point compared with that of D. latum. Nucleotide sequence differences between D. latum and the specimens from Koreans represented 17.3% in mitochondrial DNA cox1 gene. Sequence divergence of ITS1 among 4 Korean isolates was 0.3% and similarity was 99.7% with D. nihonkaiense and D. klebanovskii. All of the Korean specimens analyzed in this study were identified as being D. nihonkaiense (n = 62). We propose its Korean name as "Dong-hae-gin-chon-chung" which means 'long tapeworm of the East Sea' for this newly analyzed diphyllobothriid tapeworm in Korea.

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Mini Reviews

Geographical Distribution of Taenia asiatica and Related Species
Keeseon S. Eom, Hyeong-Kyu Jeon, Han-Jong Rim
Korean J Parasitol 2009;47(Suppl):S115.
Published online October 27, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.S.S115

Geographical information of Taenia asiatica is reviewed together with that of T. solium and T. saginata. Current distribution of T. asiatica was found to be mostly from Asian countries: the Republic of Korea, China, Taiwan, Indonesia, and Thailand. Molecular genotypic techniques have found out more countries with T. asiatica from Japan, the Philippines, and Vietnam. Specimens used in this paper were collected from around the world and mostly during international collaboration projects of Korean foundations for parasite control activities (1995-2009) in developing countries.

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Foodborne Intestinal Flukes in Southeast Asia
Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2009;47(Suppl):S69.
Published online October 23, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.S.S69

In Southeast Asia, a total of 59 species of foodborne intestinal flukes have been known to occur in humans. The largest group is the family Heterophyidae, which constitutes 22 species belonging to 9 genera (Centrocestus, Haplorchis, Heterophyes, Heterophyopsis, Metagonimus, Procerovum, Pygidiopsis, Stellantchasmus, and Stictodora). The next is the family Echinostomatidae, which includes 20 species in 8 genera (Artyfechinostomum, Acanthoparyphium, Echinochasmus, Echinoparyphium, Echinostoma, Episthmium, Euparyphium, and Hypoderaeum). The family Plagiorchiidae follows the next containing 5 species in 1 genus (Plagiorchis). The family Lecithodendriidae includes 3 species in 2 genera (Phaneropsolus and Prosthodendrium). In 9 other families, 1 species in 1 genus each is involved; Cathaemaciidae (Cathaemacia), Fasciolidae (Fasciolopsis), Gastrodiscidae (Gastrodiscoides), Gymnophallidae (Gymnophalloides), Microphallidae (Spelotrema), Neodiplostomidae (Neodiplostomum), Paramphistomatidae (Fischoederius), Psilostomidae (Psilorchis), and Strigeidae (Cotylurus). Various types of foods are sources of human infections. They include freshwater fish, brackish water fish, fresh water snails, brackish water snails (including the oyster), amphibians, terrestrial snakes, aquatic insects, and aquatic plants. The reservoir hosts include various species of mammals or birds.The host-parasite relationships have been studied in Metagonimus yokogawai, Echinostoma hortense, Fasciolopsis buski, Neodiplostomum seoulense, and Gymnophalloides seoi; however, the pathogenicity of each parasite species and host mucosal defense mechanisms are yet poorly understood. Clinical aspects of each parasite infection need more clarification. Differential diagnosis by fecal examination is difficult because of morphological similarity of eggs. Praziquantel is effective for most intestinal fluke infections. Continued efforts to understand epidemiological significance of intestinal fluke infections, with detection of further human cases, are required.

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Original Articles

Fishborne Trematode Metacercariae in Freshwater Fish from Guangxi Zhuang Autonomous Region, China
Woon-Mok Sohn, Keeseon S. Eom, Duk-Young Min, Han-Jong Rim, Eui-Hyug Hoang, Yichao Yang, Xueming Li
Korean J Parasitol 2009;47(3):249-257.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.249

A survey was performed to investigate the infection status of fishborne trematode (FBT) metacercariae in freshwater fish from Guangxi Zhuang Autonomous Region, China. A total of 307 freshwater fish of 31 species were collected from 5 administrative regions of Guangxi Zhuang Autonomous Region. They were examined by artificial digestion method from July 2003 to August 2004. No metacercariae were detected in fish from Fusui-xian. In fish from Mashan-xian and a market in Nanning, 3 species of metacercariae, Haplorchis taichui, Haplorchis pumilio, and Centrocestus formosanus, were mainly detected. Metacercariae (8 in number) of Clonorchis sinensis were found in 1 Chanodichthys dabryi purchased from a market in Nanning. In fish from Yangshuo, Metagonimus yokogawai metacercariae were detected from all 18 fish species examined. Total 13 C. sinensis metacercariae were found in 3 out of 10 Hemibarbus maculatus from Yangshuo. All 7 Zacco platypus from Yangshuo were infected with 8-112 Echinochasmus perfoliatus metacercariae. In fish from Binyang-xian, H. pumilo metacercariae were mainly detected in all 5 fish species examined, and only 1 metacercaria of C. sinensis was found in a Hemiculter leucisculus. From the above results, it was confirmed that some species of freshwater fish play a role of second intermediate hosts for FBT in Guangxi Zhuang Autonomous Region, China. In particular, 4 species of intestinal flukes, M. yokogawai, H. taichui, H. pumilio, and C. formosanus, were prevalent in fish hosts, whereas C. sinensis metacercariae were detected only in 3 fish species.

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High Prevalence of Haplorchis taichui, Phaneropsolus molenkampi, and Other Helminth Infections among People in Khammouane Province, Lao PDR
Jong-Yil Chai, Eun-Taek Han, Eun-Hee Shin, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Jin-Young Um, Min-Sung Park, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2009;47(3):243-247.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.243

The prevalence of liver and intestinal helminth infections, including Opisthorchis, Haplorchis, Phaneropsolus, hookworms, Enterobius, and Taenia, was surveyed in Khammouane province, Lao PDR. Fecal specimens were collected from 1,242 people (590 men and 652 women) in 3 Mekong riverside villages and were examined by the Kato-Katz thick smear technique. The overall helminth egg positive rate was 81.1%. The positive rate for small trematode eggs, including Opisthorchis viverrini, heterophyids, and lecithodendriids, was 81.1% and the positive rate for hookworms was 6.7%. To obtain adult worms, 35 people who were positive for small trematode eggs were treated with 20-30 mg/kg praziquantel and 10-15 mg/kg pyrantel pamoate, and then purged. Diarrheic stools were collected from 33 of these people and searched for helminth parasites using a stereomicroscope. Mixed infections with various helminths (Haplorchis taichui, Haplorchis yokogawai, Prosthodendrium molenkampi, Phaneropsolus bonnei, echinostomes, hookworms, Trichostrongylus spp., Trichuris trichiura, Enterobius vermicularis, and/or Taenia saginata) were found. The total number of helminth specimens collected was 20,907 (approximately 634 per person). The most common species was H. taichui, followed by P. molenkampi, O. viverrini, P. bonnei, E. vermicularis, hookworms, and Trichostrongylus spp. These results show that diverse species of intestinal nematodes, trematodes, and cestodes are infecting humans in Khammouane province, Lao PDR.

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Indochinamon ou (Crustacea: Potamidae) as a New Second Intermediate Host for Paragonimus harinasutai in Luang Prabang Province, Lao PDR
Woon-Mok Sohn, Jae-Sook Ryu, Duk-Young Min, Hyun-Ouk Song, Han-Jong Rim, Youthanavanh Vonghachack, Daluny Bouakhasith, Virasack Banouvong
Korean J Parasitol 2009;47(1):25-29.
Published online March 12, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.1.25

Paragonimus harinasutai metacercariae were found in a species of freshwater crab, Indochinamon ou, collected in a small stream of Namback District, Luang Prabang Province, Lao PDR. Adult flukes were recovered after experimental infection of the metacercariae to dogs. Metacercariae were round or slightly elliptical, 0.666 × 0.626 mm in average size, and had a thin cyst wall of about 20 ?m in thickness, a black excretory bladder, convoluted ceca, and some pinkish materials in the body. Adults were somewhat elongated, 95.2 × 36.5 mm in average size, covered with single-tipped tegumental spines, had a smaller oral sucker than the ventral sucker, a moderately branched ovary, and 5-6 lobulated testes. Eggs were ovoid and bilaterally symmetrical in shape, 79 × 45 ?m in average size, and had a uniformly thickened shell. By the present study, it has been confirmed that I. ou is a new second intermediate host for P. harinasutai.

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Fishborne Trematode Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR
Han-Jong Rim, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Jong-Yil Chai, Duk-Young Min, Soon-Hyung Lee, Eui-Hyug Hoang, Bounlay Phommasack, Sithat Insisengmay
Korean J Parasitol 2008;46(4):253-260.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.253

Freshwater fish from Vientiane Municipality and Savannakhet Province, Lao PDR were examined by the muscle compression and artificial digestion methods to know the infection status with trematode metacercariae. In the fish from Savannakhet, 2 species of metacercariae, Opisthorchis viverrini and Haplorchis taichui, were detected. O. viverrini metacercariae were found in 6 species of fish, Puntius brevis, Hampala dispar, Esomus metallicus, Mystacoleucus marginatus, Puntioplites falcifer, and Cyclocheilichthys armatus. H. taichui metacercariae were detected in 3 species of fish, P. brevis, P. falcifer, and M. marginatus. In the fish from Vientiane, 4 species of metacercariae, O. viverrini, H. taichui, Haplorchis yokogawai, and Centrocestus formosanus, were detected. Among them, O. viverrini metacercariae were found in 7 species of fish, Onychostoma elongatum, C. armatus, H. dispar, P. brevis, Cyclocheilichthys repasson, Osteochilus hasseltii, and Hypsibarbus lagleri. The metacercariae of H. taichui were detected in 6 species of fish, C. repasson, O. elongatum, C. armatus, H. dispar, Labiobarbus leptocheila, and Cirrhinus molitorella. The metacercariae of H. yokogawai were found in 9 species of fish, C. repasson, O. elongatum, C. armatus, H. dispar, Labiobarbus leptocheila, O. hasseltii. C. molitorella, Hypsibarbus wetmorei, and H. lagleri. The metacercariae of C. formosanus were detected in 4 species of fish, C. repasson, P. brevis, O. hasseltii, and C. molitorella. From these results, it is confirmed that fishborne trematode metacercariae, i.e. O. viverrini, H. taichui, H. yokogawai and C. formosanus, are prevalent in various species of freshwater fish from Savannakhet Province and Vientiane Municipality, Lao PDR.

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Sympatric Distribution of Three Human Taenia Tapeworms Collected between 1935 and 2005 in Korea
Hyeong-Kyu Jeon, Kyu-Heon Kim, Jong-Yil Chai, Hyun-Jong Yang, Han-Jong Rim, Keeseon S Eom
Korean J Parasitol 2008;46(4):235-241.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.235

Taeniasis has been known as one of the prevalent parasitic infections in Korea. Until recently, Taenia saginata had long been considered a dominant, and widely distributed species but epidemiological profiles of human Taenia species in Korea still remain unclear. In order to better understand distribution patterns of human Taenia tapeworms in Korea, partial nucleotide sequences of mitochondrial cox1 and ITS2 (internal transcribed spacer 2) were determined, along with morphological examinations, on 68 Taenia specimens obtained from university museum collections deposited since 1935. Genomic DNA was extracted from formalin-preserved specimens. Phylogenetic relationships among the genotypes (cox1 haplotype) detected in this study were inferred using the neighbor-joining method as a tree building method. Morphological and genetic analyses identified 3 specimens as T. solium, 51 specimens as T. asiatica, and 14 specimens as T. saginata. Our results indicate that all 3 Taenia tapeworms are sympatrically distributed in Korea with T. asiatica dominating over T. saginata and T. solium.

Citations

Citations to this article as recorded by  Crossref logo
  • Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in India
    Aman D. Moudgil, Anil K. Nehra, Pallavi Moudgil
    New Microbes and New Infections.2025; 63: 101562.     CrossRef
  • Four Taeniasis saginata Cases Diagnosed at a University Hospital in Korea
    Eun Jeong Won, Ju Hyeon Shin, Yu Jeong Lee, Moon-Ju Kim, Seung Ji Kang, Sook In Jung, Soo Hyun Kim, Jong Hee Shin, Jong-Yil Chai, Sung-Shik Shin
    The Korean Journal of Parasitology.2019; 57(3): 313.     CrossRef
  • Ten Cases of Taenia saginata Infection Confirmed by Analysis of the Internal Transcribed Spacer 1 rDNA Region in the Republic of Korea
    Su-Min Song, Hae Soo Yun, Dorene VanBik, Hyun-Ha Chang, Sang-Ah Lee, Shin-Woo Kim, Namhee Ryoo, Dong Yeub Eun, Nan Young Lee, Youn-Kyoung Goo, Yeonchul Hong, Meesun Ock, Hee-Jae Cha, Dong-Il Chung
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  • Historical Details about the Meat Consumption and Taeniases in Joseon Period of Korea
    Dong Hoon Shin, Jong-Yil Chai, Jong Ha Hong, Min Seo
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  • Immunology ofTaenia soliumtaeniasis and human cysticercosis
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  • Four Cases of Taenia saginata Infection with an Analysis of COX1 Gene
    Jaeeun Cho, Bong-Kwang Jung, Hyemi Lim, Min-Jae Kim, Thanapon Yooyen, Dongmin Lee, Keeseon S. Eom, Eun-Hee Shin, Jong-Yil Chai
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  • Evolution, molecular epidemiology and perspectives on the research of taeniid parasites with special emphasis on Taenia solium
    Raúl J. Bobes, Gladis Fragoso, Agnès Fleury, Martín García-Varela, Edda Sciutto, Carlos Larralde, Juan P. Laclette
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  • High Prevalence of Haplorchis taichui, Phaneropsolus molenkampi, and Other Helminth Infections among People in Khammouane Province, Lao PDR
    Jong-Yil Chai, Eun-Taek Han, Eun-Hee Shin, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Jin-Young Um, Min-Sung Park, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Soon-Hyung Lee, Han-Jong Rim
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Brief Communications

Skin Test for Paragonimiasis among Schoolchildren and Villagers in Namback District, Luangprabang Province, Lao PDR
Hyun-Ouk Song, Duk-Young Min, Han-Jong Rim, Vonghachack Youthanavanh, Bouakhasith Daluny, Vongsouvan Sengdara, Banouvong Virasack, Phommasak Bounlay
Korean J Parasitol 2008;46(3):179-182.
Published online September 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.3.179

As a part of a broader effort to determine the status of Paragonimus species infection in Lao PDR, an epidemiological survey was conducted on villagers and schoolchildren in Namback District between 2003 and 2005. Among 308 villagers and 633 primary and secondary schoolchildren, 156 villagers and 92 children evidenced a positive reaction on a Paragonimus skin test. Only 4 schoolchildren out of 128 skin test-positive cases had Paragonimus sp. eggs in their sputum, all of which was collected on 1 day. Several types of crabs, which were identified as the second intermediate host of the Paragonimus species, were collected from markets and streams in a paragonimiasis endemic area for the inspection of metacercariae. Among the examined crabs, only "rock crabs" (Indochinamon ou) harbored Paragonimus sp. metacercariae, and it is speculated that the life cycle of Paragonimus sp. was maintained via rock crabs in Namback District, Lao PDR.

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Centrocestus formosanus (Digenea: Heterophyidae) Encysted in the Freshwater Fish, Puntius brevis, from Lao PDR
Eun-Taek Han, Eun-Hee Shin, Souvanny Phommakorn, Bounthong Sengvilaykham, Jae-Lip Kim, Han-Jong Rim, Jong-Yil Chai
Korean J Parasitol 2008;46(1):49-53.
Published online March 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.1.49

The metacercariae of Centrocestus formosanus, a minute intestinal trematode of mammals and birds, were detected in the freshwater fish, Puntius brevis, from Vientiane Municipality, Lao PDR. The metacercariae were experimentally fed to mice, and adult flukes were recovered in their small intestines 7 days later. The adult flukes were morphologically characterized by having 32 (rarely 34) circumoral spines arranged in 2 alternative rows, a large bipartite seminal vesicle, an oval-shaped ovary, and an X-shaped excretory bladder. Based on these characters, the adults were identified as Centrocestus formosanus (Nishigori, 1924). The taxonomic significance of C. formosanus, in relation to a closely related species, C. caninus (Leiper, 1913), is briefly discussed. It has been first verified by adult worm recovery that C. formosanus is prevalent in Vientiane areas of Lao PDR, taking the freshwater fish, P. brevis, as a second intermediate host.

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Original Articles

High prevalence of liver and intestinal fluke infections among residents of Savannakhet Province in Laos
Jong-Yil Chai, Eun-Taek Han, Sang-Mee Guk, Eun-Hee Shin, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Keon-Hoon Lee, Hoo-Gn Jeong, Yong-Sang Ryang, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2007;45(3):213-218.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.213

The prevalence of liver and intestinal fluke infections was surveyed on residents of Savannakhet Province, Laos. Fecal specimens were collected from a total of 981 residents in 4 Mekong riverside villages and examined by the Kato-Katz thick smear technique. The results revealed that the overall helminth egg positive rate was 84.2%, and the positive rate for small trematode eggs, including Opisthorchis viverrini, heterophyids, or lecithodendriids, was 67.1%. To obtain adult flukes, 38 small trematode egg positive cases were treated with a 20-30 mg/kg single dose of praziquantel and purged. Diarrheic stools were then collected from 29 people and searched for helminth parasites using stereomicroscopes. Mixed infections with O. viverrini and 6 kinds of intestinal flukes were found, namely, Haplorchis taichui, Haplorchis pumilio, Haplorchis yokogawai, Prosthodendrium molenkampi, Phaneropsolus bonnei, and echinostomes. The total number of flukes collected was 7,693 specimens (av. no. per treated person; 265.3). The most common species was O. viverrini, followed by H. taichui, P. molenkampi, echinostomes, H. pumilio, P. bonnei, and H. yokogawai. The results indicate that foodborne liver and intestinal fluke infections are prevalent among residents of Savannakhet Province, Laos.

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A survey of Heterophyes nocens and Pygidiopsis summa metacercariae in mullets and gobies along the coastal areas of the Republic of Korea
Sang-Mee Guk, Eun-Hee Shin, Jae-Lip Kim, Woon-Mok Sohn, Kwang-Sun Hong, Cheong-Ha Yoon, Soon-Hyung Lee, Han-Jong Rim, Jong-Yil Chai
Korean J Parasitol 2007;45(3):205-211.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.205

The infection status of mullets Mugil cephalus (n = 139) and gobies (n = 35) Acanthogobius flavimanus with metacercariae of Heterophyes nocens and Pygidiopsis summa was examined in 11 western, southern, and eastern coastal areas of the Republic of Korea, using a digestion technique. Heterophyid metacercariae were highly prevalent in mullets from western and southern coastal areas; Shinan-gun (100% for H. nocens and 100% for P. summa), Muan-gun (93% and 100%), Buan-gun (42% and 75%), Seocheon-gun (73% and 53%), Ganghwa-gun (47% and 100%), Sacheon-shi (47% and 77%), and Gangjin-gun (50% and 70%, respectively). Only 1 (10%) of 10 mullets from an eastern coastal area, i.e., Donghae-shi, was positive for P. summa metacercariae. Metacercarial densities were the highest in the trunk of mullets for H. nocens and the gill for P. summa. Gobies from Muan-gun were positive for H. nocens (40%) and P. summa metacercariae (40%), and gobies from Seocheon-gun revealed H. nocens metacercariae (20%). The metacercarial density was remarkably higher in mullets than in gobies. The results revealed that H. nocens and P. summa metacercariae are prevalent in mullets and gobies from coastal areas of the Republic of Korea, and the prevalence and intensity of infection vary according to geographical locality.

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Brief Communications

Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence
Tai-Soon Yong, Jong-Ho Lee, Seobo Sim, Jongweon Lee, Duk-Young Min, Jong-Yil Chai, Keeseon S. Eom, Woon-Mok Sohn, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2007;45(1):69-74.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.69

Trichostrongylus eggs observed in cellophane-thick smears are difficult, in practice, to distinguish from hookworm eggs. In order to overcome these limitations, a molecular approach was conducted. A Trichostrongylus colubriformis adult worm was obtained from a human in Laos, which was identified morphologically. ITS-1 sequence of this worm was determined, and found to be most similar with that of T. colubriformis among the Trichostrongylus spp. reported so far. Then, this sequence was compared with those of human hookworm species, Ancylostoma duodenale and Necator americanus, and species-specific oligonucleotide primers were designed. Polymerase chain reaction (PCR) using these primers evidenced specifically amplified PCR products of Trichostrongylus sp., A. duodenale and N. americanus from the eggs of each (520 bp, 690 bp, and 870 bp, respectively). A species-specific PCR technique can be developed in order to study the epidemiology of Trichostrongylus spp. and hookworms in endemic areas.

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A survey of cryptosporidiosis among 2,541 residents of 25 coastal islands in Jeollanam-do (Province), Republic of Korea
Jae-Hwan Park, Hyo-Jin Kim, Sang-Mee Guk, Eun-Hee Shin, Jae-Lip Kim, Han-Jong Rim, Soon-Hyung Lee, Jong-Yil Chai
Korean J Parasitol 2006;44(4):367-372.
Published online December 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.4.367

In order to determine the distribution and prevalence of human cryptosporidiosis on western and southern coastal islands of Jeollanam-do (Province), fecal samples were collected from 2,541 people residing on 25 islands, 13 in the western coasts and 12 in the southern coasts, during July and August 2000. Fecal smears were prepared following formalin-ether sedimentation of the samples and stained by a modified acid-fast procedure. The presence of Cryptosporidium oocysts was determined by light microscopy. Cryptosporidium oocysts were detected in 38 specimens (1.5%). The oocyst positive rate varied (0-6.0%) according to island; the highest was detected on Oenarodo (6.0%), followed by Naenarodo (5.6%) and Nakwoldo (5.4%). The majority (35 persons, 94.6%) of Cryptosporidium-infected individuals were older than 50 years of age. Men (22/1,159; 1.9%) were infected at a higher rate than women (16/1,382; 1.2%). The results of the present survey indicate that human Cryptosporidium infections (due to Cryptosporidium hominis and/or C. parvum) are maintained at a relatively low prevalence on coastal islands of Jeollanam-do, Republic of Korea.

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Mini Review

A successful experience of soil-transmitted helminth control in the Republic of Korea
Sung-Tae Hong, Jong-Yil Chai, Min-Ho Choi, Sun Huh, Han-Jong Rim, Soon-Hyung Lee
Korean J Parasitol 2006;44(3):177-185.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.177

Soil-transmitted helminths (STH), namely Ascaris, Trichuris and hookworms (Ancylostoma and Necator), present a global health problem to about a half of the earth's population. In the Republic of Korea, STH were highly prevalent and were considered a high priority target for national control. To promote the control, a non-governmental organization named Korea Association for Parasite Eradication (currently Korea Association of Health Promotion) was founded in 1964, and mass fecal examination followed by selective mass chemotherapy with anthelmintics was performed twice a year from 1969 to 1995 targeting whole nationwide schoolchildren. Meanwhile, decreasing patterns of national STH infections have been monitored by 7 times' quinquennial national surveys targeting general population. In 1971, the overall intestinal helminth egg positive rate was 84.3% (Ascaris 58.2%, Trichuris 65.4%, and hookworms 10.7%), which became 63.2% in 1976, 41.1% in 1981, 12.9% in 1986, 3.8% in 1992, 2.4% in 1997, and 4.3% (Ascaris 0.03%, Trichuris 0.02%, and hookworms 0%) in 2004. During the control period, national economy rapidly developed, and living standards including environment, sanitation, and agricultural technology greatly improved, which undoubtedly boosted the STH control effects. Our experience indicates that social driving force to establish an eligible national control system to conduct repeated mass chemotherapy, together with improvement of environment and sanitation, is important for initiating and achieving STH control in a developing community.

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Original Article

A seroepidemiological survey of Taenia solium cysticercosis in Nabo, Guangxi Zhuang Autonomous Region, China
Joon-Yong Chung, Keeseon S. Eom, Yichao Yang, Xenming Li, Zheng Feng, Han-Jong Rim, Seung-Yull Cho, Yoon Kong
Korean J Parasitol 2005;43(4):135-139.
Published online December 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.4.135

We have observed the seropositive rate of Taenia solium cysticercosis in residents at Nabo Village, Tiandong County, Guangxi Zhuang Autonomous Region, China by enzyme linked immunosorbent assay. The village had been found to be a relatively high endemic area of porcine cysticercosis among roaming pigs. Of 202 persons examined four males aged 15, 25, 35 and 41 year-old exhibited absorbance (abs) at 0.18, 0.20, 0.35 and 0.55, respectively. In addition, two females whose ages were 35 and 39 years revealed specific antibody levels of abs 0.26 and 0.41 in their sera. Overall positive rate among the people was 2.97%. All of these persons agreed that they had ingested the pork infected with T. solium metacestode (TsM), while history of proglottid discharge was not noticed from all of them. Three males and one female complained of intermittent headache. Our findings reinforced not only that the prevalence of cysticercosis might be related with roaming pigs infected with TsM but also that behavioral and environmental practices in local community constituted risk factors for transmission of the infection.

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  • A Marked Decline in Taenia solium Taeniasis and Cysticercosis Infections in China: Possible Reasons from the Ecological Determinants Perspective
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    Akira Ito, Tiaoying Li, Toni Wandra, Paron Dekumyoy, Tetsuya Yanagida, Munehiro Okamoto, Christine M Budke
    Acta Tropica.2019; 198: 105075.     CrossRef
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Brief Communication

A survey of Brugia malayi infection on the Heugsan Islands, Korea
Jong-Yil Chai, Soon-Hyung Lee, Sung-Yil Choi, Jong-Soo Lee, Tai-Soon Yong, Kyun-Jong Park, Kyeong-Ae Yang, Keon-Hoon Lee, Mi-Jeng Park, Hyung-Ran Park, Mi-Ja Kim, Han-Jong Rim
Korean J Parasitol 2003;41(1):69-73.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.69

Lymphatic filariasis due to Brugia malayi infection was endemic in several areas of South Korea. The infection was controlled, or disappeared, in most areas, with the exception of the remote southwestern islands of Jeonranam-do, including the Heugsan Islands. To discover its current situation, a small-scale survey was performed on the Heugsan Islands in September 2000. A total of 378 people, 151 male and 227 female, living in 8 villages (6 on Daeheugsan-do, 1 on Daejang-do, and 1 on Yeongsan-do) were subjected to a night blood survey for microfilaremia, and physical examination for elephantiasis on the extremities. There were 6 (1.6%) microfilaria positive cases, all in females aged 57-72 years, and from only two villages of the Daeheugsan-do area. There were 4 patients with lower leg elephantiasis, but they showed no microfilaremia. The results show that a low-grade endemicity of filariasis remains on the Daeheugsan-do.

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    Shin Hyeong Cho, Da Won Ma, Bo Ra Koo, Hee Eun Shin, Wook Kyo Lee, Byong Suk Jeong, Chaeshin Chu, Won Ja Lee, Hyeng Il Cheun
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Original Article

Control of clonorchiasis by repeated treatments with praziquantel
Sung-Tae Hong, Han-Jong Rim, Duk-Young Min, Xueming Li, Jingxian Xu, Zheng Feng, Soon-Hyung Lee
Korean J Parasitol 2001;39(4):285-292.
Published online December 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.4.285

The present study aimed to evaluate control efficacy of clonorchiasis by two schemes of repeated treatment with praziquantel at two endemic villages In China. Residents of one village at Guangxi Autonomous Region were treated and examined 6-monthly and of another at Liaoning Province 12-monthly. In residents that took 25 mg/kg x3 (total 75 mg/kg) of praziquantel every 6 months for one year the egg positive rate showed a significant drop from 69.0% to 17.1%. In contrast, a group of same praziquantel medication once showed a slight marginal decrease in the egg rate from 18.9% to 12.2% after one year. Of 39 subjects examined 3 times, 56.4% were cured, 7.7% persistently positive, one (2.6%) reinfected after cure or newly infected, but 25.6% were persistently negative. The present finding suggests that 6-monthly medication with 75 mg/kg of praziquantel should effectively lower the prevalence but incomplete for control of clonorchiasis in heavy endemic areas.

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Mini Review

Epidemiological understanding of Taenia tapeworm infections with special reference to Taenia asiatica in Korea
Keeseon S. Eom, Han-Jong Rim
Korean J Parasitol 2001;39(4):267-283.
Published online December 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.4.267

In endemic areas of Taenia tapeworms in Korea, most of the reports showed that T. saginata was dominant over T. solium, but eating pigs is the dominant habit over eating cattle. Why do they have more T. saginata despite lower consumption of beef? This problem actually has long been recognized but until recently there has been no intensive trial to give a scientific explanation on this epidemiological enigma. By summing up the data published between the years 1963 and 1999, the ratio of armed versus unarmed tapeworms in humans was estimated at approximately 1:5. The ratio of pig-eaters versus cattle-eaters, however, was approximately 5:1. This inconsistency could be explained with the recently described T. asiatica, which infects humans through the eating of pig's viscera. We re-evaluate the importance of the consumption of visceral organ of pigs, leading us to an improved epidemiological understanding of the T. asiatica infection together with co-existing T. saginata and T. solium in Korea.

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Original Article

A nationwide survey of the prevalence of human Gymnophalloides seoi infection on western and southern coastal islands in the Republic of Korea
Jong-Yil Chai, Jae-Hwan Park, Eun-Taek Han, Eun-Hee Shin, Jae-Lip Kim, Kwang-Seon Hong, Han-Jong Rim, Soon-Hyung Lee
Korean J Parasitol 2001;39(1):23-30.
Published online March 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.1.23

A nationwide survey was performed to know the distribution and prevalence of human Gymnophalloides seoi infection on western and southern coastal islands in the Republic of Korea. A total of 4,178 fecal specimens were collected from residents on 45 (24 western and 21 southern) islands, and examined by Kato-Katz and formalin-ether sedimentation techniques. Eggs of G. seoi were detected from 160 (3.8%) people living on 22 (13 western and 9 southern) islands. The prevalence varied by the location of islands; higher on western islands than on southern islands. The highest prevalence was found on Amtaedo (25.3%), followed by Cheungdo (25.0%), and Anchwado (20.9%) (Shinan-gun). A little lower prevalence was observed on Munyodo (13.3%), Shinshido (12.9%), and Sonyudo (10.3%) (Kunsan-shi). Of the remaining islands, the regions showing the prevalence greater than 5% included Kohado, Dallido (Mokpo-shi), Pyeongildo, Kogumdo (Wando-gun), and Keogumdo (Kohung-gun). A strong age predilection was noted (p < 0.05); 95% of the infected people were over 40 years old. Females showed a little higher prevalence than males. The results indicate that human G. seoi infection is more widely distributed than previously considered. Nine of 11 islands (excluding the 2 known areas Munyodo and Sunyudo) that showed greater prevalence than 5% are regarded as new endemic foci of G. seoi.

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    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
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    Soon-Hyung Lee, Jong-Yil Chai
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Brief Communication
Neoplagioporus zacconis (Trematoda: Opecoelidae) from the intestine of the pale chub, Zacco platypus, in Korea
Ki Hong Kim, Jae Bum Cho, Han-Jong Rim
Korean J Parasitol 1998;36(3):199-202.
Published online September 20, 1998
DOI: https://doi.org/10.3347/kjp.1998.36.3.199

Neoplagioporus zacconis (Yamaguti, 1934) (Trematoda: Opecoelidae) was found from the intestine of the pale chub, Zacco platypus, for the first time in Korea. The worms were characterized morphologically by oval body shape, bipartited seminal vesicle, sinistrally located genital pore, and lobed ovary, and distinguished from the two other species of Neoplagioporous by body shape and distribution of vitelline follicles. The morphological characteristics, except the ovary, well corresponded to those of the previous descriptions. The morphological difference of the ovary was considered as a character of geographical phenotypic variation.

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    Misako Urabe, Eriko Higa
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