Parasites, Hosts and Diseases

"Jiraporn Kuesap"

Original Articles

Molecular Markers for Sulfadoxine/Pyrimethamine and Chloroquine Resistance in Plasmodium falciparum in Thailand: Jiraporn Kuesap, Nutnicha Suphakhonchuwong, Lertluk Kalawong, Natthaya Khumchum; Korean J Parasitol 2022;60(2):109-116.
Published online April 20, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.2.109

Abstract
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Drug resistance is an important problem hindering malaria elimination in tropical areas. Point mutations in Plasmodium falciparum dihydrofolate reductase (Pfdhfr) and dihydropteroate synthase (Pfdhps) genes confer resistance to antifolate drug, sulfadoxine-pyrimethamine (SP) while P. falciparum chloroquine-resistant transporter (Pfcrt) genes caused resistance to chloroquine (CQ). Decline in Pfdhfr/Pfdhps and Pfcrt mutations after withdrawal of SP and CQ has been reported. The aim of present study was to investigate the prevalence of Pfdhfr, Pfdhps, and Pfcrt mutation from 2 endemic areas of Thailand. All of 200 blood samples collected from western area (Thai-Myanmar) and southern area (Thai-Malaysian) contained multiple mutations in Pfdhfr and Pfdhps genes. The most prevalent haplotypes for Pfdhfr and Pfdhps were quadruple and double mutations, respectively. The quadruple and triple mutations of Pfdhfr and Pfdhps were common in western samples, whereas low frequency of triple and double mutations was found in southern samples, respectively. The Pfcrt 76T mutation was present in all samples examined. Malaria isolated from 2 different endemic regions of Thailand had high mutation rates in the Pfdhfr, Pfdhps, and Pfcrt genes. These findings highlighted the fixation of mutant alleles causing resistance of SP and CQ in this area. It is necessary to monitor the re-emergence of SP and CQ sensitive parasites in this area.

Citations

Citations to this article as recorded by

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Tameika Bagratee, Ritika Prawlall, Thabani Ndlovu, Sinqobile Sibisi, Sisa Ndadane, Baji Baba Shaik, Mahesh B. Palkar, Raghavachary Gampa, Rajshekhar Karpoormath
Chemistry & Biodiversity.2024;[Epub] CrossRef
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Pratap Vydyam, Meenal Chand, Shalev Gihaz, Isaline Renard, Gavin D. Heffernan, Laura R. Jacobus, David P. Jacobus, Kurt W. Saionz, Raju Shah, Hong-Ming Shieh, Jacek Terpinski, Wenyi Zhao, Emmanuel Cornillot, Choukri Ben Mamoun, Audrey Odom John
Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef
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Adebanjo J. Adegbola, Omotade A. Ijarotimi, Akaninyene E. Ubom, Bukola A. Adesoji, Olajide E. Babalola, Emma F. Hocke, Helle Hansson, Andria Mousa, Oluseye O. Bolaji, Michael Alifrangis, Cally Roper
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European Journal of Medicinal Chemistry.2023; 259: 115699. CrossRef

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The Effect of ABO Blood Groups, Hemoglobinopathy, and Heme Oxygenase-1 Polymorphisms on Malaria Susceptibility and Severity: Jiraporn Kuesap, Kesara Na-Bangchang; Korean J Parasitol 2018;56(2):167-173.
Published online April 30, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.2.167

Abstract
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Malaria is one of the most important public health problems in tropical areas on the globe. Several factors are associated with susceptibility to malaria and disease severity, including innate immunity such as blood group, hemoglobinopathy, and heme oxygenase-1 (HO-1) polymorphisms. This study was carried out to investigate association among ABO blood group, thalassemia types and HO-1 polymorphisms in malaria. The malarial blood samples were collected from patients along the Thai-Myanmar border. Determination of ABO blood group, thalassemia variants, and HO-1 polymorphisms were performed using agglutination test, low pressure liquid chromatography and polymerase chain reaction, respectively. Plasmodium vivax was the major infected malaria species in the study samples. Distribution of ABO blood type in the malaria-infected samples was similar to that in healthy subjects, of which blood type O being most prevalent. Association between blood group A and decreased risk of severe malaria was significant. Six thalassemia types (30%) were detected, i.e., hemoglobin E (HbE), β-thalassemia, α-thalassemia 1, α-thalassemia 2, HbE with α-thalassemia 2, and β-thalassemia with α-thalassemia 2. Malaria infected samples without thalassemia showed significantly higher risk to severe malaria. The prevalence of HO-1 polymorphisms, S/S, S/L and L/L were 25, 62, and 13%, respectively. Further study with larger sample size is required to confirm the impact of these 3 host genetic factors in malaria patients.

Citations

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Nibedita Mitra, Prosanto Chowdhury, Anupam Basu
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Yahye Isse Hassan, Mohamed Said Hassan
Health Science Reports.2025;[Epub] CrossRef
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Genotyping of ABO and Duffy blood groups among malaria patients in Thailand
Phattharaphon Hongfongfa, Jiraporn Kuesap
Journal of Parasitic Diseases.2022; 46(1): 178. CrossRef
HMOX1 genetic polymorphisms and outcomes in infectious disease: A systematic review
Fergus W. Hamilton, Julia Somers, Ruth E. Mitchell, Peter Ghazal, Nicholas J. Timpson, Srinivas Mummidi
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Contribution of genetic factors to high rates of neonatal hyperbilirubinaemia on the Thailand-Myanmar border
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Azeez Oyemomi Ibrahim, Ibrahim Sebutu Bello, Olabode Muftau Shabi, Adejumoke Oluwatosin Omonijo, Abayomi Ayodapo, Babatunde Adeola Afolabi
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HMOX1 STR polymorphism and malaria: an analysis of a large clinical dataset
Fergus Hamilton, Ruth Mitchell, Aubrey Cunnington, Peter Ghazal, Nicholas J. Timpson
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Linkages between blood groups and malaria susceptibility
Minu Nain, Amit Sharma
Journal of Vector Borne Diseases.2022; 59(3): 193. CrossRef
AB Blood Group Confers Higher Risk for Primary Brain Tumors in Pediatrics
Abdulrahman Al Shudifat, Hala Al Suqi, Kutada Soub, Leen Al Nemrawi, Moa’tasem Abu Jaber, Mohammad Al Barbarawi, Nour Shewaikani, Yazan El Adwan, Assem Al Refaei
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High susceptibility to severe malaria among patients with A blood group versus those with O blood group
Serge Tonen-Wolyec, Salomon Batina-Agasa
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Effects of IgG and IgM autoantibodies on non-infected erythrocytes is related to ABO blood group in Plasmodium vivax malaria and is associated with anemia
Luiza Carvalho Mourão, Camila Maia Pantuzzo Medeiros, Gustavo Pereira Cardoso-Oliveira, Paula Magda da Silva Roma, Jamila da Silva Sultane Aboobacar, Beatriz Carolina Medeiros Rodrigues, Ubirajara Agero, Cor Jesus Fernandes Fontes, Érika Martins Braga
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There will be blood
Sophia Häfner
Microbes and Infection.2020; 22(9): 385. CrossRef

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Coexistence of Malaria and Thalassemia in Malaria Endemic Areas of Thailand: Jiraporn Kuesap, W. Chaijaroenkul, K. Rungsihirunrat, K. Pongjantharasatien, Kesara Na-Bangchang; Korean J Parasitol 2015;53(3):265-270.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.265

Abstract
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Hemoglobinopathy and malaria are commonly found worldwide particularly in malaria endemic areas. Thalassemia, the alteration of globin chain synthesis, has been reported to confer resistance against malaria. The prevalence of thalassemia was investigated in 101 malaria patients with Plasmodium falciparum and Plasmodium vivax along the Thai-Myanmar border to examine protective effect of thalassemia against severe malaria. Hemoglobin typing was performed using low pressure liquid chromatography (LPLC) and α-thalassemia was confirmed by multiplex PCR. Five types of thalassemia were observed in malaria patients. The 2 major types of thalassemia were Hb E (18.8%) and α-thalassemia-2 (11.9%). There was no association between thalassemia hemoglobinopathy and malaria parasitemia, an indicator of malaria disease severity. Thalassemia had no significant association with P. vivax infection, but the parasitemia in patients with coexistence of P. vivax and thalassemia was about 2-3 times lower than those with coexistence of P. falciparum and thalassemia and malaria without thalassemia. Furthermore, the parasitemia of P. vivax in patients with coexistence of Hb E showed lower value than coexistence with other types of thalassemia and malaria without coexistence. Parasitemia, hemoglobin, and hematocrit values in patients with coexistence of thalassemia other than Hb E were significantly lower than those without coexistence of thalassemia. Furthermore, parasitemia with coexistence of Hb E were 2 times lower than those with coexistence of thalassemia other than Hb E. In conclusion, the results may, at least in part, support the protective effect of thalassemia on the development of hyperparasitemia and severe anemia in malaria patients.

Citations

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Brief Communication

Genetic Polymorphisms in Plasmodium vivax Dihydrofolate Reductase and Dihydropteroate Synthase in Isolates from the Philippines, Bangladesh, and Nepal: Pimwan Thongdee, Jiraporn Kuesap, Kanchana Rungsihirunrat, Shyam Prakash Dumre, Effie Espino, Harald Noedl, Kesara Na-Bangchang; Korean J Parasitol 2015;53(2):227-232.
Published online April 22, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.2.227

Abstract
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Genetic polymorphisms of pvdhfr and pvdhps genes of Plasmodium vivax were investigated in 83 blood samples collected from patients in the Philippines, Bangladesh, and Nepal. The SNP-haplotypes of the pvdhfr gene at the amino acid positions 13, 33, 57, 58, 61, 117, and 173, and that of the pvdhps gene at the positions 383 and 553 were analyzed by nested PCR-RFLP. Results suggest diverse polymorphic patterns of pvdhfr alone as well as the combination patterns with pvdhps mutant alleles in P. vivax isolates collected from the 3 endemic countries in Asia. All samples carried mutant combination alleles of pvdhfr and pvdhps. The most prevalent combination alleles found in samples from the Philippines and Bangladesh were triple mutant pvdhfr combined with single mutant pvdhps allele and triple mutant pvdhfr combined with double wild-type pvdhps alleles, respectively. Those collected from Nepal were quadruple mutant pvdhfr combined with double wild-type pvdhps alleles. New alternative antifolate drugs which are effective against sulfadoxine-pyrimethamine (SP)-resistant P. vivax are required.

Citations

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Original Articles

Plasmodium vivax Drug Resistance Genes; Pvmdr1 and Pvcrt-o Polymorphisms in Relation to Chloroquine Sensitivity from a Malaria Endemic Area of Thailand: Kanchana Rungsihirunrat, Poonuch Muhamad, Wanna Chaijaroenkul, Jiraporn Kuesap, Kesara Na-Bangchang; Korean J Parasitol 2015;53(1):43-49.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.43

Abstract
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The aim of the study was to explore the possible molecular markers of chloroquine resistance in Plasmodium vivax isolates in Thailand. A total of 30 P. vivax isolates were collected from a malaria endemic area along the Thai-Myanmar border in Mae Sot district of Thailand. Dried blood spot samples were collected for analysis of Pvmdr1 and Pvcrt-o polymorphisms. Blood samples (100 μl) were collected by finger-prick for in vitro chloroquine susceptibility testing by schizont maturation inhibition assay. Based on the cut-off IC₅₀ of 100 nM, 19 (63.3%) isolates were classified as chloroquine resistant P. vivax isolates. Seven non-synonymous mutations and 2 synonymous were identified in Pvmdr1 gene. Y976F and F1076L mutations were detected in 7 (23.3%) and 16 isolates (53.3%), respectively. Analysis of Pvcrt-o gene revealed that all isolates were wild-type. Our results suggest that chloroquine resistance gene is now spreading in this area. Monitoring of chloroquine resistant molecular markers provide a useful tool for future control of P. vivax malaria.

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Nested-PCR and a New ELISA-Based NovaLisa Test Kit for Malaria Diagnosis in an Endemic Area of Thailand: Pimwan Thongdee, Wanna Chaijaroenkul, Jiraporn Kuesap, Kesara Na-Bangchang; Korean J Parasitol 2014;52(4):377-381.
Published online August 29, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.4.377

Abstract
PDF

Microscopy is considered as the gold standard for malaria diagnosis although its wide application is limited by the requirement of highly experienced microscopists. PCR and serological tests provide efficient diagnostic performance and have been applied for malaria diagnosis and research. The aim of this study was to investigate the diagnostic performance of nested PCR and a recently developed an ELISA-based new rapid diagnosis test (RDT), NovaLisa test kit, for diagnosis of malaria infection, using microscopic method as the gold standard. The performance of nested-PCR as a malaria diagnostic tool is excellent with respect to its high accuracy, sensitivity, specificity, and ability to discriminate Plasmodium species. The sensitivity and specificity of nested-PCR compared with the microscopic method for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax mixed infection were 71.4 vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa test kit compared with the microscopic method for detection of Plasmodium genus were 89.0 vs 91.6%, respectively. NovaLisa test kit provided comparable diagnostic performance. Its relatively low cost, simplicity, and rapidity enables large scale field application.

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Accuracy of diagnosis among clinical malaria patients: comparing microscopy, RDT and a highly sensitive quantitative PCR looking at the implications for submicroscopic infections
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Brief Communication

Evolution of Genetic Polymorphisms of Plasmodium falciparum Merozoite Surface Protein (PfMSP) in Thailand: Jiraporn Kuesap, Wanna Chaijaroenkul, Kanchanok Ketprathum, Puntanat Tattiyapong, Kesara Na-Bangchang; Korean J Parasitol 2014;52(1):105-109.
Published online February 19, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.1.105

Abstract
PDF

Plasmodium falciparum malaria is a major public health problem in Thailand due to the emergence of multidrug resistance. The understanding of genetic diversity of malaria parasites is essential for developing effective drugs and vaccines. The genetic diversity of the merozoite surface protein-1 (PfMSP-1) and merozoite surface protein-2 (PfMSP-2) genes was investigated in a total of 145 P. falciparum isolates collected from Mae Sot District, Tak Province, Thailand during 3 different periods (1997-1999, 2005-2007, and 2009-2010). Analysis of genetic polymorphisms was performed to track the evolution of genetic change of P. falciparum using PCR. Both individual genes and their combination patterns showed marked genetic diversity during the 3 study periods. The results strongly support that P. falciparum isolates in Thailand are markedly diverse and patterns changed with time. These 2 polymorphic genes could be used as molecular markers to detect multiple clone infections and differentiate recrudescence from reinfection in P. falciparum isolates in Thailand.

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Yannick Johnson, Ahmad Rushdi Shakri, Sunthorn Pond-Tor, Anup Jnawali, Tanbir Najrana, Haiwei Wu, Jhasketan Badhai, Mohamad-Gabriel Alameh, Drew Weissman, Edward Kabyemela, Patrick Duffy, Michal Fried, Jonathan Kurtis, Dipak Kumar Raj
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Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
Mary A. Oboh, Tolla Ndiaye, Khadim Diongue, Yaye D. Ndiaye, Mouhamad Sy, Awa B. Deme, Mamadou A. Diallo, Mamadou S. Yade, Sarah K. Volkman, Aida S. Badiane, Alfred Amambua-Ngwa, Daouda Ndiaye
Malaria Journal.2021;[Epub] CrossRef
Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region
Cang-Lin Zhang, Hong-Ning Zhou, Quan Liu, Ya-Ming Yang
Malaria Journal.2019;[Epub] CrossRef

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Original Article

Possible Role of Heme Oxygenase-1 and Prostaglandins in the Pathogenesis of Cerebral Malaria: Heme Oxygenase-1 Induction by Prostaglandin D₂ and Metabolite by a Human Astrocyte Cell Line: Jiraporn Kuesap, Kesara Na-Bangchang; Korean J Parasitol 2010;48(1):15-21.
Published online March 17, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.1.15

Abstract
PDF

Astrocytes are the most abundant cells in the central nervous system that play roles in maintaining the blood-brain-barrier and in neural injury, including cerebral malaria, a severe complication of Plasmodium falciparum infection. Prostaglandin (PG) D₂ is abundantly produced in the brain and regulates the sleep response. Moreover, PGD₂ is a potential factor derived from P. falciparum within erythrocytes. Heme oxygenase-1 (HO-1) is catalyzing enzyme in heme breakdown process to release iron, carbon monoxide, and biliverdin/bilirubin, and may influence iron supply to the P. falciparum parasites. Here, we showed that treatment of a human astrocyte cell line, CCF-STTG1, with PGD₂ significantly increased the expression levels of HO-1 mRNA by RT-PCR. Western blot analysis showed that PGD₂ treatment increased the level of HO-1 protein, in a dose- and time-dependent manner. Thus, PGD₂ may be involved in the pathogenesis of cerebral malaria by inducing HO-1 expression in malaria patients.

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