Parasites, Hosts and Diseases

"Kesara Na-Bangchang"

Brief Communication

Monitoring antimalarial drug-resistance markers in Somalia: Abdifatah Abdullahi Jalei, Kesara Na-Bangchang, Phunuch Muhamad, Wanna Chaijaroenkul; Parasites Hosts Dis 2023;61(1):78-83.
Published online February 22, 2023; DOI: https://doi.org/10.3347/PHD.22140

Abstract
PDF

The use of an effective antimalarial drug is the cornerstone of malaria control. However, the development and spread of resistant Plasmodium falciparum strains have placed the global eradication of malaria in serious jeopardy. Molecular marker analysis constitutes the hallmark of the monitoring of Plasmodium drug-resistance. This study included 96 P. falciparum PCR-positive samples from southern Somalia. The P. falciparum chloroquine resistance transporter gene had high frequencies of K76T, A220S, Q271E, N326S, and R371I point mutations. The N86Y and Y184F mutant alleles of the P. falciparum multidrug resistance 1 gene were present in 84.7 and 62.4% of the isolates, respectively. No mutation was found in the P. falciparum Kelch-13 gene. This study revealed that chloroquine resistance markers are present at high frequencies, while the parasite remains sensitive to artemisinin (ART). The continuous monitoring of ART-resistant markers and in vitro susceptibility testing are strongly recommended to track resistant strains in real time.

Citations

Citations to this article as recorded by

Preventive interventions and diagnostic testing compliance in the management of tropical infections among patients with sickle cell disease in Tanzania
Felician Paschal Mgasa, Singeun Oh, Avelina Mgasa, Sunjoo Kang, Erasto Mbugi, Ju Yeong Kim
Parasites, Hosts and Diseases.2025; 63(2): 147. CrossRef
Influence of genetic factors of humans, mosquitoes and parasites, on the evolution of Plasmodium falciparum infections, malaria transmission and genetic control methods: a review of the literature
Seni Nikiema, Issiaka Soulama, Gifty Dufie Ampofo, Moustapha Nikiema, Abdou Azaque Zouré, Salif Sombié, Salam Sawadogo, Nicolas Ouedraogo, Samuel Sindie Sermé, Haffsatou Sawadogo, Raïssa Ily, Guillène Y. N. Tibiri, Djamila O. A. Zouré, Nassandba Julien Ya
BMC Medical Genomics.2025;[Epub] CrossRef
Investigating the relationship between Pfkelch13 mutations and response to artemisinin-based treatment for uncomplicated falciparum malaria: a protocol for a systematic review and individual patient data meta-analysis
Stephanie van Wyk, Prabin Dahal, Chistevy Vouvoungui, Dhol S Ayuen, Farhad Shokraneh, Aboubakar Soma, James A Watson, Philippe Guerin, Karen I Barnes
BMJ Open.2025; 15(7): e100251. CrossRef
Understanding the global rise of artemisinin resistance: Insights from over 100,000 Plasmodium falciparum samples
Andrew J Balmer, Nina FD White, Eyyüb S Ünlü, Chiyun Lee, Richard D Pearson, Jacob Almagro-Garcia, Cristina Ariani
eLife.2025;[Epub] CrossRef
Emergence of Plasmodium falciparum strains with artemisinin partial resistance in East Africa and the Horn of Africa: is there a need to panic?
Ashenafi Assefa, Abebe A. Fola, Geremew Tasew
Malaria Journal.2024;[Epub] CrossRef
Therapeutic efficacy and safety of artemether-lumefantrine for uncomplicated Plasmodium falciparum malaria treatment in Metehara, Central-east Ethiopia
Mahelet Tesfaye, Ashenafi Assefa, Henok Hailgiorgis, Bokretsion Gidey, Hussein Mohammed, Getachew Tollera, Geremew Tasew, Gudissa Assefa, Worku Bekele, Hassen Mamo
Malaria Journal.2024;[Epub] CrossRef
Update on antimicrobial resistance in Somalia: Current status, challenges, opportunities, and future perspectives
Shafie Abdulkadir Hassan, Ahmed Mohamed Dirie, Nur Rashid Ahmed, Abdifetah Ibrahim Omar
Heliyon.2024; 10(20): e39434. CrossRef
Emerging threat of artemisinin partial resistance markers (pfk13 mutations) in Plasmodium falciparum parasite populations in multiple geographical locations in high transmission regions of Uganda
Bosco B. Agaba, Jye Travis, David Smith, Simon P. Rugera, Maria G. Zalwango, Jimmy Opigo, Charles Katureebe, Ruth Mpirirwe, Dembo Bakary, Martin Antonio, Beshir Khalid, Joseph Ngonzi, Moses R. Kamya, Pontiano Kaleebu, Peter Piot, Qin Cheng
Malaria Journal.2024;[Epub] CrossRef
Geospatial Analysis of Malaria Burden in Kagera Region, Northwestern Tanzania Using Health Facility and Community Survey Data
Daniel A Petro, Nyimvua Shaban, Sijenunu Aaron, Frank Chacky, Samuel Lazaro, Maciej F Boni, Deus S Ishengoma
Open Forum Infectious Diseases.2024;[Epub] CrossRef

4,603 View
180 Download
8 Web of Science
Crossref

Original Articles

The Effect of ABO Blood Groups, Hemoglobinopathy, and Heme Oxygenase-1 Polymorphisms on Malaria Susceptibility and Severity: Jiraporn Kuesap, Kesara Na-Bangchang; Korean J Parasitol 2018;56(2):167-173.
Published online April 30, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.2.167

Abstract
PDF

Malaria is one of the most important public health problems in tropical areas on the globe. Several factors are associated with susceptibility to malaria and disease severity, including innate immunity such as blood group, hemoglobinopathy, and heme oxygenase-1 (HO-1) polymorphisms. This study was carried out to investigate association among ABO blood group, thalassemia types and HO-1 polymorphisms in malaria. The malarial blood samples were collected from patients along the Thai-Myanmar border. Determination of ABO blood group, thalassemia variants, and HO-1 polymorphisms were performed using agglutination test, low pressure liquid chromatography and polymerase chain reaction, respectively. Plasmodium vivax was the major infected malaria species in the study samples. Distribution of ABO blood type in the malaria-infected samples was similar to that in healthy subjects, of which blood type O being most prevalent. Association between blood group A and decreased risk of severe malaria was significant. Six thalassemia types (30%) were detected, i.e., hemoglobin E (HbE), β-thalassemia, α-thalassemia 1, α-thalassemia 2, HbE with α-thalassemia 2, and β-thalassemia with α-thalassemia 2. Malaria infected samples without thalassemia showed significantly higher risk to severe malaria. The prevalence of HO-1 polymorphisms, S/S, S/L and L/L were 25, 62, and 13%, respectively. Further study with larger sample size is required to confirm the impact of these 3 host genetic factors in malaria patients.

Citations

Citations to this article as recorded by

Exploring the functional and immune landscape of E-β thalassemia patients through RNA sequencing of peripheral blood mononuclear cells
Nibedita Mitra, Prosanto Chowdhury, Anupam Basu
Heliyon.2025; 11(1): e41255. CrossRef
Prevalence and Association of Malaria With ABO Blood Groups in Bosaso City, Puntland, Somalia: A Cross‐Sectional Study
Yahye Isse Hassan, Mohamed Said Hassan
Health Science Reports.2025;[Epub] CrossRef
Malaria infection and its association with socio-demographics, long lasting insecticide nets usage and hematological parameters among adolescent patients in rural Southwestern Nigeria
Azeez Oyemomi IBRAHIM, Tosin Anthony Agbesanwa, Shuaib Kayode AREMU, Ibrahim Sebutu BELLO, Olayide Toyin ELEGBEDE, Olusegun Emmanuel GABRIEL-ALAYODE, Oluwaserimi Adewumi AJETUNMOBI, Kayode Rasaq ADEWOYE, Temitope Moronkeji OLANREWAJU, Ebenezer Kayode ARIY
PLOS ONE.2023; 18(7): e0287723. CrossRef
Histidine-rich protein II nanoparticle delivery of heme iron load drives endothelial inflammation in cerebral malaria
Suong T. Nguyen, Daniel Du, Daniel Wychrij, Matthew D. Cain, Qingping Wu, Robyn S. Klein, Ilaria Russo, Daniel E. Goldberg
Proceedings of the National Academy of Sciences.2023;[Epub] CrossRef
Genotyping of ABO and Duffy blood groups among malaria patients in Thailand
Phattharaphon Hongfongfa, Jiraporn Kuesap
Journal of Parasitic Diseases.2022; 46(1): 178. CrossRef
HMOX1 genetic polymorphisms and outcomes in infectious disease: A systematic review
Fergus W. Hamilton, Julia Somers, Ruth E. Mitchell, Peter Ghazal, Nicholas J. Timpson, Srinivas Mummidi
PLOS ONE.2022; 17(5): e0267399. CrossRef
The Clinical Utility of ABO and RHD Systems as Potential Indicators of Health Status, a Preliminary Study in Greek Population
Evgenia Lymperaki, Evangelia Stalika, George Tzavelas, Efthymia Tormpantoni, Diana Samara, Eleni Vagdatli, Ioannis Tsamesidis
Clinics and Practice.2022; 12(3): 406. CrossRef
Contribution of genetic factors to high rates of neonatal hyperbilirubinaemia on the Thailand-Myanmar border
Germana Bancone, Gornpan Gornsawun, Pimnara Peerawaranun, Penporn Penpitchaporn, Moo Kho Paw, Day Day Poe, December Win, Naw Cicelia, Mavuto Mukaka, Laypaw Archasuksan, Laurence Thielemans, Francois Nosten, Nicholas J. White, Rose McGready, Verena I. Carr
PLOS Global Public Health.2022; 2(6): e0000475. CrossRef
Malaria infection and its association with socio-demographics, preventive measures, and co-morbid ailments among adult febrile patients in rural Southwestern Nigeria: A cross-sectional study
Azeez Oyemomi Ibrahim, Ibrahim Sebutu Bello, Olabode Muftau Shabi, Adejumoke Oluwatosin Omonijo, Abayomi Ayodapo, Babatunde Adeola Afolabi
SAGE Open Medicine.2022;[Epub] CrossRef
HMOX1 STR polymorphism and malaria: an analysis of a large clinical dataset
Fergus Hamilton, Ruth Mitchell, Aubrey Cunnington, Peter Ghazal, Nicholas J. Timpson
Malaria Journal.2022;[Epub] CrossRef
Linkages between blood groups and malaria susceptibility
Minu Nain, Amit Sharma
Journal of Vector Borne Diseases.2022; 59(3): 193. CrossRef
AB Blood Group Confers Higher Risk for Primary Brain Tumors in Pediatrics
Abdulrahman Al Shudifat, Hala Al Suqi, Kutada Soub, Leen Al Nemrawi, Moa’tasem Abu Jaber, Mohammad Al Barbarawi, Nour Shewaikani, Yazan El Adwan, Assem Al Refaei
Risk Management and Healthcare Policy.2021; Volume 14: 4031. CrossRef
High susceptibility to severe malaria among patients with A blood group versus those with O blood group
Serge Tonen-Wolyec, Salomon Batina-Agasa
Tropical Parasitology.2021; 11(2): 97. CrossRef
Effects of IgG and IgM autoantibodies on non-infected erythrocytes is related to ABO blood group in Plasmodium vivax malaria and is associated with anemia
Luiza Carvalho Mourão, Camila Maia Pantuzzo Medeiros, Gustavo Pereira Cardoso-Oliveira, Paula Magda da Silva Roma, Jamila da Silva Sultane Aboobacar, Beatriz Carolina Medeiros Rodrigues, Ubirajara Agero, Cor Jesus Fernandes Fontes, Érika Martins Braga
Microbes and Infection.2020; 22(8): 379. CrossRef
There will be blood
Sophia Häfner
Microbes and Infection.2020; 22(9): 385. CrossRef

11,236 View
196 Download
12 Web of Science
Crossref

Coexistence of Malaria and Thalassemia in Malaria Endemic Areas of Thailand: Jiraporn Kuesap, W. Chaijaroenkul, K. Rungsihirunrat, K. Pongjantharasatien, Kesara Na-Bangchang; Korean J Parasitol 2015;53(3):265-270.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.265

Abstract
PDF

Hemoglobinopathy and malaria are commonly found worldwide particularly in malaria endemic areas. Thalassemia, the alteration of globin chain synthesis, has been reported to confer resistance against malaria. The prevalence of thalassemia was investigated in 101 malaria patients with Plasmodium falciparum and Plasmodium vivax along the Thai-Myanmar border to examine protective effect of thalassemia against severe malaria. Hemoglobin typing was performed using low pressure liquid chromatography (LPLC) and α-thalassemia was confirmed by multiplex PCR. Five types of thalassemia were observed in malaria patients. The 2 major types of thalassemia were Hb E (18.8%) and α-thalassemia-2 (11.9%). There was no association between thalassemia hemoglobinopathy and malaria parasitemia, an indicator of malaria disease severity. Thalassemia had no significant association with P. vivax infection, but the parasitemia in patients with coexistence of P. vivax and thalassemia was about 2-3 times lower than those with coexistence of P. falciparum and thalassemia and malaria without thalassemia. Furthermore, the parasitemia of P. vivax in patients with coexistence of Hb E showed lower value than coexistence with other types of thalassemia and malaria without coexistence. Parasitemia, hemoglobin, and hematocrit values in patients with coexistence of thalassemia other than Hb E were significantly lower than those without coexistence of thalassemia. Furthermore, parasitemia with coexistence of Hb E were 2 times lower than those with coexistence of thalassemia other than Hb E. In conclusion, the results may, at least in part, support the protective effect of thalassemia on the development of hyperparasitemia and severe anemia in malaria patients.

Citations

Citations to this article as recorded by

Changes in Lipid Profile Secondary to Asymptomatic Malaria in Migrants from Sub-Saharan Africa: A Retrospective Analysis of a 2010–2022 Cohort
Diego Gayoso-Cantero, María Dolores Corbacho-Loarte, Clara Crespillo-Andújar, Sandra Chamorro-Tojeiro, Francesca Norman, Jose A. Perez-Molina, Marta González-Sanz, Oihane Martín, José Miguel Rubio, Beatriz Gullón-Peña, Laura del Campo Albendea, Rogelio Ló
Tropical Medicine and Infectious Disease.2025; 10(5): 134. CrossRef
Prevalence of the Main Human Genetic Variants Related to Resistance to Malaria in a Population of the Colombian Pacific Coast
Diana Carolina Ortega, María Paula Arango, Sergio Cañón, Heiber Cárdenas, Ranulfo González, Guillermo Barreto
Annals of Human Genetics.2025;[Epub] CrossRef
Human genetic variations conferring resistance to malaria
Xiaokun Zhang, Jie Wu, Yunxing Peng, Lan Luo, Lu Zhang, Xi Huang, Guoying Chen, Yirong Li, Haoan Yi
Journal of Translational Medicine.2025;[Epub] CrossRef
Abordaje sindrómico en el paciente inmigrante: fiebre, diarrea, anemia, eosinofilia y tos crónica
Agustín Francisco Rossetti, Sara Obelleiro Nadal, Federica Gutierrez de Quijano Miceli, Julia Garcia-Gozalbes, Ana I. Jiménez Lozano, Núria Serre-Delcor
Atención Primaria.2024; 56(8): 102924. CrossRef
Hematological changes due to malaria – An update
Rana Hussein Naser, Toktam Rajaii, Bibi Razieh Hosseini Farash, Seyyed javad Seyyedtabaei, Vahid Hajali, Fatemeh Sadabadi, Ehsan Saburi
Molecular and Biochemical Parasitology.2024; 259: 111635. CrossRef
Batı Ege Bölgesinde α-Talasemi Genotipleri ve α-Talasemi Genotip Frekansı
Savaş BARIŞ, Cüneyd YAVAŞ, Özgür BALASAR, Zülfükar GÖRDÜ, Mustafa DOĞAN, Recep ERÖZ
Sağlık Bilimlerinde Değer.2023; 13(2): 257. CrossRef
Primate malarias as a model for cross-species parasite transmission
Marina Voinson, Charles L Nunn, Amy Goldberg
eLife.2022;[Epub] CrossRef
Vivax Malaria and the Potential Role of the Subtelomeric Multigene vir Superfamily
Youn-Kyoung Goo
Microorganisms.2022; 10(6): 1083. CrossRef
A new investigative strategy to diagnose β-thalassemia syndrome in past human populations
Filippo Scianò, Barbara Bramanti, Emanuela Gualdi-Russo
Archaeological and Anthropological Sciences.2021;[Epub] CrossRef
Consequences of β-Thalassemia or Sickle Cell Disease for Ovarian Follicle Number and Morphology in Girls Who Had Ovarian Tissue Cryopreserved
Linn Salto Mamsen, Stine Gry Kristensen, Susanne Elisabeth Pors, Jane Alrø Bøtkjær, Erik Ernst, Kirsten Tryde Macklon, Debra Gook, Ajay Kumar, Bhanu Kalra, Claus Yding Andersen
Frontiers in Endocrinology.2021;[Epub] CrossRef
A Systematic Review and Meta-Analysis on the Epidemiology of Hepatitis B and Hepatitis C Virus among Beta-Thalassemia Major Patients in Pakistan
Usman Waheed, Noore Saba, Akhlaaq Wazeer, Saeed Ahmed
Journal of Laboratory Physicians.2021; 13(03): 270. CrossRef
Putative pathogen-selected polymorphisms in the PKLR gene are associated with mycobacterial susceptibility in Brazilian and African populations
Ohanna Cavalcanti de Lima Bezerra, Lucia Elena Alvarado-Arnez, Nédio Mabunda, Graça Salomé, Amina de Sousa, Fernanda de Souza Gomes Kehdy, Carolinne Sales-Marques, Fernanda Saloum de Neves Manta, Rafaela Mota Andrade, Laís Pereira Ferreira, Thyago Leal-Ca
PLOS Neglected Tropical Diseases.2021; 15(8): e0009434. CrossRef
Beta thalassemia minor is a beneficial determinant of red blood cell storage lesion
Vassilis L. Tzounakas, Alkmini T. Anastasiadi, Davide Stefanoni, Francesca Cendali, Lorenzo Bertolone, Fabia Gamboni, Monika Dzieciatkowska, Pantelis Rousakis, Athina Vergaki, Vassilis Soulakis, Ourania E. Tsitsilonis, Konstantinos Stamoulis, Issidora S.
Haematologica.2021; 107(1): 112. CrossRef
Associations between red blood cell variants and malaria among children and adults from three areas of Uganda: a prospective cohort study
Elijah Kakande, Bryan Greenhouse, Francis Bajunirwe, Chris Drakeley, Joaniter I. Nankabirwa, Andrew Walakira, Samuel L. Nsobya, Agaba Katureebe, John Rek, Emmanuel Arinaitwe, Philip J. Rosenthal, Moses R. Kamya, Grant Dorsey, Isabel Rodriguez-Barraquer
Malaria Journal.2020;[Epub] CrossRef
An insight into Indonesian current thalassaemia care and challenges
Pustika Amalia Wahidiyat, Teny Tjitra Sari, Ludi Dhyani Rahmartani, Iswari Setianingsih, Stephen Diah Iskandar, Anastasia Michelle Pratanata, Ivana Yapiy, Mikhael Yosia, Fernando Tricta
ISBT Science Series.2020; 15(3): 334. CrossRef
Prevalence of Alpha(α)-Thalassemia in Southeast Asia (2010–2020): A Meta-Analysis Involving 83,674 Subjects
Lucky Poh Wah Goh, Eric Tzyy Jiann Chong, Ping-Chin Lee
International Journal of Environmental Research and Public Health.2020; 17(20): 7354. CrossRef
Health-related quality of life (HRQoL) in beta-thalassemia major (β-TM) patients assessed by 36-item short form health survey (SF-36): a meta-analysis
Mahdieh Arian, Majid Mirmohammadkhani, Raheb Ghorbani, Mohsen Soleimani
Quality of Life Research.2019; 28(2): 321. CrossRef
Comparison of gene mutation spectrum of thalassemia in different regions of China and Southeast Asia
Zhuo Yang, Quexuan Cui, Wenzhe Zhou, Ling Qiu, Bing Han
Molecular Genetics & Genomic Medicine.2019;[Epub] CrossRef
Molecular prevalence of thalassemia and hemoglobinopathies among the Lao Loum Group in the Lao People’s Democratic Republic
Sayphonh Phanmany, Supantitra Chanprasert, Thongperm Munkongdee, Saovaros Svasti, Kamonlak Leecharoenkiat
International Journal of Laboratory Hematology.2019; 41(5): 650. CrossRef
Hemoglobin E, malaria and natural selection
Jiwoo Ha, Ryan Martinson, Sage K Iwamoto, Akihiro Nishi
Evolution, Medicine, and Public Health.2019; 2019(1): 232. CrossRef
Ethnic and Geographical Aspects of the Prevalence of the Polymorphic Variants of Genes Associated with Tuberculosis
N. P. Babushkina, A. N. Kucher, E. Yu. Bragina, A. F. Garaeva, I. A. Goncharova, D. Yu. Tcitrikov, D. E. Gomboeva, A. A. Rudko, M. B. Freidin
Russian Journal of Genetics.2018; 54(9): 1089. CrossRef
Novel Tag SNPs of Beta-Globin Gene Cluster in Chinese Han Population: Biological Marker for Genetic Backgrounds and Clinical Studies
Wittaya Jomoui
International Journal of Human Genetics.2017; 17(3): 97. CrossRef
Prevalence of Thalassemia and Glucose-6-Phosphate Dehydrogenase Deficiency in Newborns and Adults at the Ramathibodi Hospital, Bangkok, Thailand
Wansa Banyatsuppasin, Sumalee Jindadamrongwech, Anchalee Limrungsikul, Punnee Butthep
Hemoglobin.2017; 41(4-6): 260. CrossRef
Hemoglobin E Prevalence among Ethnic Groups Residing in Malaria-Endemic Areas of Northern Thailand and Its Lack of Association with Plasmodium falciparum Invasion In Vitro
Pathrapol Lithanatudom, Jiraprapa Wipasa, Pitsinee Inti, Kriangkrai Chawansuntati, Saovaros Svasti, Suthat Fucharoen, Daoroong Kangwanpong, Jatupol Kampuansai, Bruce Russell
PLOS ONE.2016; 11(1): e0148079. CrossRef

13,445 View
177 Download
25 Web of Science
Crossref

Brief Communication

Genetic Polymorphisms in Plasmodium vivax Dihydrofolate Reductase and Dihydropteroate Synthase in Isolates from the Philippines, Bangladesh, and Nepal: Pimwan Thongdee, Jiraporn Kuesap, Kanchana Rungsihirunrat, Shyam Prakash Dumre, Effie Espino, Harald Noedl, Kesara Na-Bangchang; Korean J Parasitol 2015;53(2):227-232.
Published online April 22, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.2.227

Abstract
PDF

Genetic polymorphisms of pvdhfr and pvdhps genes of Plasmodium vivax were investigated in 83 blood samples collected from patients in the Philippines, Bangladesh, and Nepal. The SNP-haplotypes of the pvdhfr gene at the amino acid positions 13, 33, 57, 58, 61, 117, and 173, and that of the pvdhps gene at the positions 383 and 553 were analyzed by nested PCR-RFLP. Results suggest diverse polymorphic patterns of pvdhfr alone as well as the combination patterns with pvdhps mutant alleles in P. vivax isolates collected from the 3 endemic countries in Asia. All samples carried mutant combination alleles of pvdhfr and pvdhps. The most prevalent combination alleles found in samples from the Philippines and Bangladesh were triple mutant pvdhfr combined with single mutant pvdhps allele and triple mutant pvdhfr combined with double wild-type pvdhps alleles, respectively. Those collected from Nepal were quadruple mutant pvdhfr combined with double wild-type pvdhps alleles. New alternative antifolate drugs which are effective against sulfadoxine-pyrimethamine (SP)-resistant P. vivax are required.

Citations

Citations to this article as recorded by

Molecular surveillance of antifolate drug resistance markers in Plasmodium vivax from Khyber Pakhtunkhwa province, northwest Pakistan
Thu Hằng Nguyễn, Hương Giang Lê, Tuấn Cường Võ, Đăng Thùy Dương Nguyễn, Kim Oanh Nguyễn, Minkyoung Cho, Youn-Kyoung Goo, Sahib Gul Afridi, Byoung-Kuk Na
Acta Tropica.2025; 264: 107583. CrossRef
Genetic Diversity of Plasmodium vivax Merozoite Surface Protein-3 Alpha and Beta from Diverse Geographic Areas of Thailand
Jiraporn Kuesap, Kanchana Rungsihirunrat, Wanna Chaijaroenkul, Mathirut Mungthin
Japanese Journal of Infectious Diseases.2022; 75(3): 241. CrossRef
Geographical spread and structural basis of sulfadoxine-pyrimethamine drug-resistant malaria parasites
Rini Chaturvedi, Jyoti Chhibber-Goel, Ishika Verma, Sreehari Gopinathan, Suhel Parvez, Amit Sharma
International Journal for Parasitology.2021; 51(7): 505. CrossRef
Molecular monitoring of dihydrofolatereductase (dhfr) and dihydropteroatesynthetase (dhps) associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates of Palawan, Philippines
Alison Paolo Bareng, Fe Esperanza Espino, Wanna Chaijaroenkul, Kesara Na-Bangchang
Acta Tropica.2018; 180: 81. CrossRef
Mutations of pvdhfr and pvdhps genes in vivax endemic-malaria areas in Kota Marudu and Kalabakan, Sabah
Umi Rubiah Sastu, Noor Rain Abdullah, Nor Azrina Norahmad, Muhammad Nor Farhan Saat, Prem Kumar Muniandy, Jenarun Jelip, Moizin Tikuson, Norsalleh Yusof, Hasidah Mohd Sidek
Malaria Journal.2016;[Epub] CrossRef
Geographic distribution of amino acid mutations in DHFR and DHPS in Plasmodium vivax isolates from Lao PDR, India and Colombia
Naowarat Saralamba, Supatchara Nakeesathit, Mayfong Mayxay, Paul N. Newton, Lyda Osorio, Jung-Ryong Kim, Nicholas J. White, Nicholas P. J. Day, Arjen M. Dondorp, Mallika Imwong
Malaria Journal.2016;[Epub] CrossRef

9,191 View
111 Download
6 Web of Science
Crossref

Original Articles

Plasmodium vivax Drug Resistance Genes; Pvmdr1 and Pvcrt-o Polymorphisms in Relation to Chloroquine Sensitivity from a Malaria Endemic Area of Thailand: Kanchana Rungsihirunrat, Poonuch Muhamad, Wanna Chaijaroenkul, Jiraporn Kuesap, Kesara Na-Bangchang; Korean J Parasitol 2015;53(1):43-49.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.43

Abstract
PDF

The aim of the study was to explore the possible molecular markers of chloroquine resistance in Plasmodium vivax isolates in Thailand. A total of 30 P. vivax isolates were collected from a malaria endemic area along the Thai-Myanmar border in Mae Sot district of Thailand. Dried blood spot samples were collected for analysis of Pvmdr1 and Pvcrt-o polymorphisms. Blood samples (100 μl) were collected by finger-prick for in vitro chloroquine susceptibility testing by schizont maturation inhibition assay. Based on the cut-off IC₅₀ of 100 nM, 19 (63.3%) isolates were classified as chloroquine resistant P. vivax isolates. Seven non-synonymous mutations and 2 synonymous were identified in Pvmdr1 gene. Y976F and F1076L mutations were detected in 7 (23.3%) and 16 isolates (53.3%), respectively. Analysis of Pvcrt-o gene revealed that all isolates were wild-type. Our results suggest that chloroquine resistance gene is now spreading in this area. Monitoring of chloroquine resistant molecular markers provide a useful tool for future control of P. vivax malaria.

Citations

Citations to this article as recorded by

Indigenous Plasmodium vivax upsurge in the Eastern Mediterranean, Western Pacific, and South East Asia regions – beyond the constant culpability of climate change, COVID-19, and armed conflicts
Loick P. Kojom Foko, Amit Sharma
International Journal for Parasitology.2025;[Epub] CrossRef
Low Genetic Diversity of Plasmodium vivax Circumsporozoite Surface Protein in Clinical Isolates from Southern Thailand
Tachin Khulmanee, Thanyapit Thita, Kanyanan Kritsiriwutinan, Usa Boonyuen, Aminoh Saai, Kanjana Inkabjan, Rimi Chakrabarti, Pradipsinh K. Rathod, Srivicha Krudsood, Mathirut Mungthin, Rapatbhorn Patrapuvich
Tropical Medicine and Infectious Disease.2024; 9(5): 94. CrossRef
Drug resistance markers in Plasmodium vivax isolates from a Kanchanaburi province, Thailand between January to May 2023
Thanawat Sridapan, Paweesuda Rattanakoch, Kaewkanha Kijprasong, Suttipat Srisutham, Kristan Alexander Schneider
PLOS ONE.2024; 19(7): e0304337. CrossRef
Investigation of Mutations in the crt-o and mdr1 Genes of Plasmodium vivax for the Molecular Surveillance of Chloroquine Resistance in Parasites from Gold Mining Areas in Roraima, Brazil
Jacqueline de Aguiar Barros, Fabiana Granja, Rebecca de Abreu-Fernandes, Lucas Tavares de Queiroz, Daniel da Silva e Silva, Arthur Camurça Citó, Natália Ketrin Almeida-de-Oliveira Mocelin, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz
Microorganisms.2024; 12(8): 1680. CrossRef
Molecular epidemiology of potential candidate markers for chloroquine resistance in imported Plasmodium vivax malaria cases in Iran
Sakineh Pirahmadi, Shima Afzali, Akram Abouie Mehrizi, Abbasali Raz, Ahmad Raeisi
Malaria Journal.2023;[Epub] CrossRef
Toxins from Animal Venoms as a Potential Source of Antimalarials: A Comprehensive Review
Zeca M. Salimo, André L. Barros, Asenate A. X. Adrião, Aline M. Rodrigues, Marco A. Sartim, Isadora S. de Oliveira, Manuela B. Pucca, Djane C. Baia-da-Silva, Wuelton M. Monteiro, Gisely C. de Melo, Hector H. F. Koolen
Toxins.2023; 15(6): 375. CrossRef
Characteristics of molecular markers associated with chloroquine resistance in Plasmodium vivax strains from vivax malaria cases in Yunnan Province, China
Hongyun Ding, Ying Dong, Yan Deng, Yanchun Xu, Yan Liu, Jing Wu, Mengni Chen, Canglin Zhang, Weibin Zheng
Malaria Journal.2023;[Epub] CrossRef
Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP
Kanyanan Kritsiriwuthinan, Warunee Ngrenngarmlert, Rapatbhorn Patrapuvich, Supaksajee Phuagthong, Kantima Choosang, Jianbing Mu
Journal of Tropical Medicine.2023; 2023: 1. CrossRef
Nationwide spatiotemporal drug resistance genetic profiling from over three decades in Indian Plasmodium falciparum and Plasmodium vivax isolates
Loick P. Kojom Foko, Geetika Narang, Jahnvi Jakhan, Suman Tamang, Amit Moun, Vineeta Singh
Malaria Journal.2023;[Epub] CrossRef
Surveillance of drug resistance molecular markers in Plasmodium vivax before and after introduction of dihydroartemisinin and piperaquine in Thailand: 2009–2019
Nutnicha Suphakhonchuwong, Kanchana Rungsihirunrat, Jiraporn Kuesap
Parasitology Research.2023; 122(12): 2871. CrossRef
Genomic analysis of Plasmodium vivax describes patterns of connectivity and putative drivers of adaptation in Ethiopia
Alebachew Messele Kebede, Edwin Sutanto, Hidayat Trimarsanto, Ernest Diez Benavente, Mariana Barnes, Richard D. Pearson, Sasha V. Siegel, Berhanu Erko, Ashenafi Assefa, Sisay Getachew, Abraham Aseffa, Beyene Petros, Eugenia Lo, Rezika Mohammed, Daniel Yil
Scientific Reports.2023;[Epub] CrossRef
Genetic Diversity of Plasmodium vivax Merozoite Surface Protein-3 Alpha and Beta from Diverse Geographic Areas of Thailand
Jiraporn Kuesap, Kanchana Rungsihirunrat, Wanna Chaijaroenkul, Mathirut Mungthin
Japanese Journal of Infectious Diseases.2022; 75(3): 241. CrossRef
Polymorphisms of potential drug resistant molecular markers in Plasmodium vivax from China–Myanmar border during 2008‒2017
Zhensheng Wang, Chunyan Wei, Yunchun Pan, Zhihua Wang, Xin Ji, Qianqian Chen, Lianhui Zhang, Zenglei Wang, Heng Wang
Infectious Diseases of Poverty.2022;[Epub] CrossRef
Global scenario of Plasmodium vivax occurrence and resistance pattern
Davinder Kaur, Shweta Sinha, Rakesh Sehgal
Journal of Basic Microbiology.2022; 62(12): 1417. CrossRef
Assessing the in vitro sensitivity with associated drug resistance polymorphisms in Plasmodium vivax clinical isolates from Delhi, India
Monika Matlani, Amit Kumar, Vineeta Singh
Experimental Parasitology.2021; 220: 108047. CrossRef
Monitoring Plasmodium vivax resistance to antimalarials: Persisting challenges and future directions
Marcelo U. Ferreira, Tais Nobrega de Sousa, Gabriel W. Rangel, Igor C. Johansen, Rodrigo M. Corder, Simone Ladeia-Andrade, José Pedro Gil
International Journal for Parasitology: Drugs and Drug Resistance.2021; 15: 9. CrossRef
Ten-Year Molecular Surveillance of Drug-Resistant Plasmodium spp. Isolated From the China–Myanmar Border
Tongke Tang, Yanchun Xu, Long Cao, Penghai Tian, Jiang Shao, Yan Deng, Hongning Zhou, Bo Xiao
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Global assessment of genetic paradigms of Pvmdr1 mutations in chloroquine-resistant Plasmodium vivax isolates
Adel Spotin, Mahmoud Mahami-Oskouei, Ehsan Ahmadpour, Mahdi Parsaei, Ali Rostami, Shima Emami, Saba Gholipour, Mostafa Farmani
Transactions of The Royal Society of Tropical Medicine and Hygiene.2020; 114(5): 339. CrossRef
Molecular Detection of Antimalarial Drug Resistance in Plasmodium vivax from Returned Travellers to NSW, Australia during 2008–2018
Chaturong Noisang, Wieland Meyer, Nongyao Sawangjaroen, John Ellis, Rogan Lee
Pathogens.2020; 9(2): 101. CrossRef
Plasmodium vivax drug resistance markers: Genetic polymorphisms and mutation patterns in isolates from Malaysia
Fei-Wen Cheong, Shairah Dzul, Mun-Yik Fong, Yee-Ling Lau, Sasheela Ponnampalavanar
Acta Tropica.2020; 206: 105454. CrossRef
Case report: recurrence of Plasmodium vivax malaria due to defective cytochrome P450 2D6 function in Pos Lenjang, Pahang, Malaysia
Noor Hafizan Mat Salleh, Mohd Faizal Abdul Rahman, Samsiah Samsusah, Jeremy Ryan De Silva, David Chun-Ern Ng, Azilawati Hanim Ghozali, Jia Hui Tan, Meng Yee Lai, Amirah Amir, Jonathan Wee Kent Liew, Yee Ling Lau
Transactions of The Royal Society of Tropical Medicine and Hygiene.2020; 114(9): 700. CrossRef
Ex vivo susceptibilities of Plasmodium vivax isolates from the China-Myanmar border to antimalarial drugs and association with polymorphisms in Pvmdr1 and Pvcrt-o genes
Jiangyan Li, Jie Zhang, Qian Li, Yue Hu, Yonghua Ruan, Zhiyong Tao, Hui Xia, Jichen Qiao, Lingwen Meng, Weilin Zeng, Cuiying Li, Xi He, Luyi Zhao, Faiza A. Siddiqui, Jun Miao, Zhaoqing Yang, Qiang Fang, Liwang Cui, Kamala Thriemer
PLOS Neglected Tropical Diseases.2020; 14(6): e0008255. CrossRef
An unlabelled probe-based real time PCR and modified semi-nested PCR as molecular tools for analysis of chloroquine resistant Plasmodium vivax isolates from Afghanistan
Sayed Hussain Mosawi, Abdolhossein Dalimi, Najibullah Safi, Reza Fotouhi-Ardakani, Fatemeh Ghaffarifar, Javid Sadraei
Malaria Journal.2020;[Epub] CrossRef
Molecular surveillance for drug resistance markers in Plasmodium vivax isolates from symptomatic and asymptomatic infections at the China–Myanmar border
Yan Zhao, Lin Wang, Myat Thu Soe, Pyae Linn Aung, Haichao Wei, Ziling Liu, Tongyu Ma, Yuanyuan Huang, Lynette J. Menezes, Qinghui Wang, Myat Phone Kyaw, Myat Htut Nyunt, Liwang Cui, Yaming Cao
Malaria Journal.2020;[Epub] CrossRef
Molecular surveillance of the Plasmodium vivax multidrug resistance 1 gene in Peru between 2006 and 2015
Fredy E. Villena, Jorge L. Maguiña, Meddly L. Santolalla, Edwar Pozo, Carola J. Salas, Julia S. Ampuero, Andres G. Lescano, Danett K. Bishop, Hugo O. Valdivia
Malaria Journal.2020;[Epub] CrossRef
Molecular detection of drug resistant malaria in Southern Thailand
Chaturong Noisang, Christiane Prosser, Wieland Meyer, Waenurama Chemoh, John Ellis, Nongyao Sawangjaroen, Rogan Lee
Malaria Journal.2019;[Epub] CrossRef
Promising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi
Yudi T. Pinilla, Stefanie C. P. Lopes, Vanderson S. Sampaio, Francys S. Andrade, Gisely C. Melo, Alessandra S. Orfanó, Nágila F. C. Secundino, Maria G. V. B. Guerra, Marcus V. G. Lacerda, Kevin C. Kobylinski, Karin S. Escobedo-Vargas, Victor M. López-Sifu
PLOS Neglected Tropical Diseases.2018; 12(2): e0006221. CrossRef
The prevalence of molecular markers of drug resistance in Plasmodium vivax from the border regions of Thailand in 2008 and 2014
Kritpaphat Tantiamornkul, Tepanata Pumpaibool, Jittima Piriyapongsa, Richard Culleton, Usa Lek-Uthai
International Journal for Parasitology: Drugs and Drug Resistance.2018; 8(2): 229. CrossRef
Genetic diversity of the Plasmodium vivax multidrug resistance 1 gene in Thai parasite populations
Veerayuth Kittichai, Wang Nguitragool, Huguette Gaelle Ngassa Mbenda, Jetsumon Sattabongkot, Liwang Cui
Infection, Genetics and Evolution.2018; 64: 168. CrossRef
Molecular Evidence of Drug Resistance in Asymptomatic Malaria Infections, Myanmar, 2015
Myat Htut Nyunt, Thinzar Shein, Ni Ni Zaw, Soe Soe Han, Fauzi Muh, Seong-Kyun Lee, Jin-Hee Han, Kyaw Zin Thant, Eun-Taek Han, Myat Phone Kyaw
Emerging Infectious Diseases.2017; 23(3): 517. CrossRef
Genetic diversity of Plasmodium vivax metacaspase 1 and Plasmodium vivax multi-drug resistance 1 genes of field isolates from Mauritania, Sudan and Oman
Fatimata Sow, Guillaume Bonnot, Bilal Rabah Ahmed, Sidi Mohamed Diagana, Hachim Kebe, Mohamedou Koita, Ba Malado Samba, Said K. Al-Mukhaini, Majed Al-Zadjali, Seif S. Al-Abri, Osama A. M. Ali, Abdallah M. Samy, Muzamil Mahdi Abdel Hamid, Musab M. Ali Albs
Malaria Journal.2017;[Epub] CrossRef
Clinical and molecular surveillance of drug resistant vivax malaria in Myanmar (2009–2016)
Myat Htut Nyunt, Jin-Hee Han, Bo Wang, Khin Myo Aye, Kyin Hla Aye, Seong-Kyun Lee, Ye Htut, Myat Phone Kyaw, Kay Thwe Han, Eun-Taek Han
Malaria Journal.2017;[Epub] CrossRef
Measuring ex vivo drug susceptibility in Plasmodium vivax isolates from Cambodia
Suwanna Chaorattanakawee, Chanthap Lon, Soklyda Chann, Kheang Heng Thay, Nareth Kong, Yom You, Siratchana Sundrakes, Chatchadaporn Thamnurak, Sorayut Chattrakarn, Chantida Praditpol, Kritsanai Yingyuen, Mariusz Wojnarski, Rekol Huy, Michele D. Spring, Dou
Malaria Journal.2017;[Epub] CrossRef
Plasmodium vivax mdr1 genotypes in isolates from successfully cured patients living in endemic and non-endemic Brazilian areas
Larissa Rodrigues Gomes, Natália Ketrin Almeida-de-Oliveira, Aline Rosa de Lavigne, Suelen Rezende Félix de Lima, Anielle de Pina-Costa, Patrícia Brasil, Cláudio Tadeu Daniel-Ribeiro, Didier Ménard, Maria de Fatima Ferreira-da-Cruz
Malaria Journal.2016;[Epub] CrossRef
Plasmodium vivax multidrug resistance-1 gene polymorphism in French Guiana
Emilie Faway, Lise Musset, Stéphane Pelleau, Béatrice Volney, Jessica Casteras, Valérie Caro, Didier Menard, Sébastien Briolant, Eric Legrand
Malaria Journal.2016;[Epub] CrossRef
Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodium vivax isolates from Republic of Korea military personnel
Dong-Il Chung, Sookwan Jeong, Sylvatrie-Danne Dinzouna-Boutamba, Hye-Won Yang, Sang-Geon Yeo, Yeonchul Hong, Youn-Kyoung Goo
Malaria Journal.2015;[Epub] CrossRef

11,525 View
195 Download
38 Web of Science
Crossref

Nested-PCR and a New ELISA-Based NovaLisa Test Kit for Malaria Diagnosis in an Endemic Area of Thailand: Pimwan Thongdee, Wanna Chaijaroenkul, Jiraporn Kuesap, Kesara Na-Bangchang; Korean J Parasitol 2014;52(4):377-381.
Published online August 29, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.4.377

Abstract
PDF

Microscopy is considered as the gold standard for malaria diagnosis although its wide application is limited by the requirement of highly experienced microscopists. PCR and serological tests provide efficient diagnostic performance and have been applied for malaria diagnosis and research. The aim of this study was to investigate the diagnostic performance of nested PCR and a recently developed an ELISA-based new rapid diagnosis test (RDT), NovaLisa test kit, for diagnosis of malaria infection, using microscopic method as the gold standard. The performance of nested-PCR as a malaria diagnostic tool is excellent with respect to its high accuracy, sensitivity, specificity, and ability to discriminate Plasmodium species. The sensitivity and specificity of nested-PCR compared with the microscopic method for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax mixed infection were 71.4 vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa test kit compared with the microscopic method for detection of Plasmodium genus were 89.0 vs 91.6%, respectively. NovaLisa test kit provided comparable diagnostic performance. Its relatively low cost, simplicity, and rapidity enables large scale field application.

Citations

Citations to this article as recorded by

Subclinical malaria among pregnant women living in refugee settlements in Northern Uganda
Winnie Kibone, Felix Bongomin, Sarah Lebu, Stephen Ochaya, Ritah Nantale, Jerom Okot, Byron Awekonimungu, Rachel Beardsley, Chimdi Muoghalu, Musa Manga
Therapeutic Advances in Infectious Disease.2024;[Epub] CrossRef
Comparative assessment of microscopy, malaria rapid diagnostic test and polymerase chain reaction as malaria diagnostic tools in Adama Woreda, East shoa zone of Ethiopia: a cross-sectional study
Getaneh Tegegn, N. Gnanasekaren, Endalamaw Gadisa, Molla Getie, Abebew Molla, Tsegaye Meharie, Habtu Debash, Ayenew Berhan, Andargachew Almaw, Aschlew Hussien, Getu Abeje
BMC Infectious Diseases.2024;[Epub] CrossRef
Accuracy of diagnosis among clinical malaria patients: comparing microscopy, RDT and a highly sensitive quantitative PCR looking at the implications for submicroscopic infections
Stephen Opoku Afriyie, Thomas Kwame Addison, Yilekal Gebre, Abdul-Hakim Mutala, Kwasi Baako Antwi, Dawood Ackom Abbas, Kofi Agyapong Addo, Austine Tweneboah, Nana Kwame Ayisi-Boateng, Cristian Koepfli, Kingsley Badu
Malaria Journal.2023;[Epub] CrossRef
Malaria therapeutics: are we close enough?
Himani Tripathi, Preshita Bhalerao, Sujeet Singh, Hemant Arya, Bader Saud Alotaibi, Summya Rashid, Mohammad Raghibul Hasan, Tarun Kumar Bhatt
Parasites & Vectors.2023;[Epub] CrossRef
Systematic Evolution of Ligands by Exponential Enrichment Technologies and Aptamer-Based Applications: Recent Progress and Challenges in Precision Medicine of Infectious Diseases
Yixin Xu, Xin Jiang, Yanhong Zhou, Ming Ma, Minjin Wang, Binwu Ying
Frontiers in Bioengineering and Biotechnology.2021;[Epub] CrossRef
Comparison of Commercial ELISA Kits to Confirm the Absence of Transmission in Malaria Elimination Settings
Lotus L. van den Hoogen, Paolo Bareng, Joana Alves, Ralph Reyes, Malou Macalinao, Júlio M. Rodrigues, José M. Fernandes, Lara F. Goméz, Tom Hall, Susheel K. Singh, Kimberly Fornace, Jennifer Luchavez, Alan Kitchen, Peter Chiodini, Fe Espino, Kevin K. A. T
Frontiers in Public Health.2020;[Epub] CrossRef
Rapid diagnostic tests forPlasmodium vivaxmalaria in endemic countries
Ridhi Agarwal, Leslie Choi, Samuel Johnson, Yemisi Takwoingi
Cochrane Database of Systematic Reviews.2020;[Epub] CrossRef
An immunosensor for parasite lactate dehydrogenase detection as a malaria biomarker – Comparison with commercial test kit
Aver Hemben, Jon Ashley, Ibtisam E. Tothill
Talanta.2018; 187: 321. CrossRef
Comparative evaluation of a rapid diagnostic test, an antibody ELISA, and a pLDH ELISA in detecting asymptomatic malaria parasitaemia in blood donors in Buea, Cameroon
Tebit Emmanuel Kwenti, Longdoh Anna Njunda, Beltine Tsamul, Shey Dickson Nsagha, Nguedia Jules-Clement Assob, Kukwah Anthony Tufon, Dilonga Henry Meriki, Enow George Orock
Infectious Diseases of Poverty.2017;[Epub] CrossRef
Identification of the Plasmodium species in clinical samples from children residing in five epidemiological strata of malaria in Cameroon
Tebit Emmanuel Kwenti, Tayong Dizzle Bita Kwenti, Longdoh Anna Njunda, Andreas Latz, Kukwah Anthony Tufon, Theresa Nkuo-Akenji
Tropical Medicine and Health.2017;[Epub] CrossRef
Current and cumulative malaria infections in a setting embarking on elimination: Amhara, Ethiopia
Woyneshet G. Yalew, Sampa Pal, Pooja Bansil, Rebecca Dabbs, Kevin Tetteh, Caterina Guinovart, Michael Kalnoky, Belendia A. Serda, Berhane H. Tesfay, Belay B. Beyene, Catherine Seneviratne, Megan Littrell, Lindsay Yokobe, Gregory S. Noland, Gonzalo J. Domi
Malaria Journal.2017;[Epub] CrossRef
Novel molecular diagnostic tools for malaria elimination: a review of options from the point of view of high-throughput and applicability in resource limited settings
Sumudu Britton, Qin Cheng, James S. McCarthy
Malaria Journal.2016;[Epub] CrossRef
Development and performance evaluation of a novel immunofluorescence chromatographic assay for histidine-rich protein 2 of Plasmodium falciparum
Keren Kang, Emmanuel E. Dzakah, Yongping Huang, Mingquan Xie, Xiaochun Luo, Wenmei Li, Jihua Wang
Malaria Journal.2015;[Epub] CrossRef

10,395 View
121 Download
14 Web of Science
Crossref

Brief Communication

Evolution of Genetic Polymorphisms of Plasmodium falciparum Merozoite Surface Protein (PfMSP) in Thailand: Jiraporn Kuesap, Wanna Chaijaroenkul, Kanchanok Ketprathum, Puntanat Tattiyapong, Kesara Na-Bangchang; Korean J Parasitol 2014;52(1):105-109.
Published online February 19, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.1.105

Abstract
PDF

Plasmodium falciparum malaria is a major public health problem in Thailand due to the emergence of multidrug resistance. The understanding of genetic diversity of malaria parasites is essential for developing effective drugs and vaccines. The genetic diversity of the merozoite surface protein-1 (PfMSP-1) and merozoite surface protein-2 (PfMSP-2) genes was investigated in a total of 145 P. falciparum isolates collected from Mae Sot District, Tak Province, Thailand during 3 different periods (1997-1999, 2005-2007, and 2009-2010). Analysis of genetic polymorphisms was performed to track the evolution of genetic change of P. falciparum using PCR. Both individual genes and their combination patterns showed marked genetic diversity during the 3 study periods. The results strongly support that P. falciparum isolates in Thailand are markedly diverse and patterns changed with time. These 2 polymorphic genes could be used as molecular markers to detect multiple clone infections and differentiate recrudescence from reinfection in P. falciparum isolates in Thailand.

Citations

Citations to this article as recorded by

Immunization with PfGBP130 generates antibodies that inhibit RBC invasion by P. falciparum parasites
Yannick Johnson, Ahmad Rushdi Shakri, Sunthorn Pond-Tor, Anup Jnawali, Tanbir Najrana, Haiwei Wu, Jhasketan Badhai, Mohamad-Gabriel Alameh, Drew Weissman, Edward Kabyemela, Patrick Duffy, Michal Fried, Jonathan Kurtis, Dipak Kumar Raj
Frontiers in Immunology.2024;[Epub] CrossRef
Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
Mary A. Oboh, Tolla Ndiaye, Khadim Diongue, Yaye D. Ndiaye, Mouhamad Sy, Awa B. Deme, Mamadou A. Diallo, Mamadou S. Yade, Sarah K. Volkman, Aida S. Badiane, Alfred Amambua-Ngwa, Daouda Ndiaye
Malaria Journal.2021;[Epub] CrossRef
Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region
Cang-Lin Zhang, Hong-Ning Zhou, Quan Liu, Ya-Ming Yang
Malaria Journal.2019;[Epub] CrossRef

9,221 View
87 Download
6 Web of Science
Crossref

Original Articles

Evaluation of Rapid Diagnostics for Plasmodium falciparum and P. vivax in Mae Sot Malaria Endemic Area, Thailand: Wanna Chaijaroenkul, Thanee Wongchai, Ronnatrai Ruangweerayut, Kesara Na-Bangchang; Korean J Parasitol 2011;49(1):33-38.
Published online March 18, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.1.33

Abstract
PDF

Prompt and accurate diagnosis of malaria is the key to prevent disease morbidity and mortality. This study was carried out to evaluate diagnostic performance of 3 commercial rapid detection tests (RDTs), i.e., Malaria Antigen Pf/Pan™, Malaria Ag-Pf™, and Malaria Ag-Pv™ tests, in comparison with the microscopic and PCR methods. A total of 460 blood samples microscopically positive for Plasmodium falciparum (211 samples), P. vivax (218), mixed with P. falciparum and P. vivax (30), or P. ovale (1), and 124 samples of healthy subjects or patients with other fever-related infections, were collected. The sensitivities of Malaria Ag-Pf™ and Malaria Antigen Pf/Pan™ compared with the microscopic method for P. falciparum or P. vivax detection were 97.6% and 99.0%, or 98.6% and 99.0%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, and Malaria Antigen Pf/Pan™ were 93.3%, 98.8%, and 94.4%, respectively. The sensitivities of Malaria Ag-Pf™, Malaria Antigen Pf/Pan™, and microscopic method, when PCR was used as a reference method for P. falciparum or P. vivax detection were 91.8%, 100%, and 96.7%, or 91.9%, 92.6%, and 97.3%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, Malaria Antigen Pf/Pan™, and microscopic method were 66.2%, 92.7%, 73.9%, and 78.2%, respectively. Results indicated that the diagnostic performances of all the commercial RDTs are satisfactory for application to malaria diagnosis.

Citations

Citations to this article as recorded by

Multiplexed quantitative proteomics provides mechanistic cues for malaria severity and complexity
Vipin Kumar, Sandipan Ray, Shalini Aggarwal, Deeptarup Biswas, Manali Jadhav, Radha Yadav, Sanjeev V. Sabnis, Soumaditya Banerjee, Arunansu Talukdar, Sanjay K. Kochar, Suvin Shetty, Kunal Sehgal, Swati Patankar, Sanjeeva Srivastava
Communications Biology.2020;[Epub] CrossRef
DIAGNOSTIC PERFORMANCE EVALUATION OF THE SD BIOLINE MALARIA ANTIGEN AG PF/PAN TEST (05FK60) IN A MALARIA ENDEMIC AREA OF SOUTHERN ETHIOPIA
Endale TADESSE, Bereket WORKALEMAHU, Techalew SHIMELIS
Revista do Instituto de Medicina Tropical de São Paulo.2016;[Epub] CrossRef
Epidemiological aspects of vivax and falciparum malaria: global spectrum
Shyamapada Mandal
Asian Pacific Journal of Tropical Disease.2014; 4: S13. CrossRef
Nested-PCR and a New ELISA-Based NovaLisa Test Kit for Malaria Diagnosis in an Endemic Area of Thailand
Pimwan Thongdee, Wanna Chaijaroenkul, Jiraporn Kuesap, Kesara Na-Bangchang
The Korean Journal of Parasitology.2014; 52(4): 377. CrossRef
Rapid diagnostic tests for diagnosing uncomplicated non-falciparum or Plasmodium vivax malaria in endemic countries
Katharine Abba, Amanda J Kirkham, Piero L Olliaro, Jonathan J Deeks, Sarah Donegan, Paul Garner, Yemisi Takwoingi
Cochrane Database of Systematic Reviews.2014;[Epub] CrossRef

9,670 View
83 Download
Crossref

Possible Role of Heme Oxygenase-1 and Prostaglandins in the Pathogenesis of Cerebral Malaria: Heme Oxygenase-1 Induction by Prostaglandin D₂ and Metabolite by a Human Astrocyte Cell Line: Jiraporn Kuesap, Kesara Na-Bangchang; Korean J Parasitol 2010;48(1):15-21.
Published online March 17, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.1.15

Abstract
PDF

Astrocytes are the most abundant cells in the central nervous system that play roles in maintaining the blood-brain-barrier and in neural injury, including cerebral malaria, a severe complication of Plasmodium falciparum infection. Prostaglandin (PG) D₂ is abundantly produced in the brain and regulates the sleep response. Moreover, PGD₂ is a potential factor derived from P. falciparum within erythrocytes. Heme oxygenase-1 (HO-1) is catalyzing enzyme in heme breakdown process to release iron, carbon monoxide, and biliverdin/bilirubin, and may influence iron supply to the P. falciparum parasites. Here, we showed that treatment of a human astrocyte cell line, CCF-STTG1, with PGD₂ significantly increased the expression levels of HO-1 mRNA by RT-PCR. Western blot analysis showed that PGD₂ treatment increased the level of HO-1 protein, in a dose- and time-dependent manner. Thus, PGD₂ may be involved in the pathogenesis of cerebral malaria by inducing HO-1 expression in malaria patients.

Citations

Citations to this article as recorded by

Heme Oxygenase 1 in Vertebrates: Friend and Foe
Rafael Cardoso Maciel Costa Silva, Leonardo Holanda Travassos Correa
Cell Biochemistry and Biophysics.2022; 80(1): 97. CrossRef
cAMP-Dependent Signaling Pathways as Potential Targets for Inhibition of Plasmodium falciparum Blood Stages
Edwin Lasonder, Kunal More, Shailja Singh, Malak Haidar, Daniela Bertinetti, Eileen J. Kennedy, Friedrich W. Herberg, Anthony A. Holder, Gordon Langsley, Chetan E. Chitnis
Frontiers in Microbiology.2021;[Epub] CrossRef
Heme oxygenase-1 in protozoan infections: A tale of resistance and disease tolerance
Rafael C. M. C. Silva, Leonardo H. Travassos, Claudia N. Paiva, Marcelo T. Bozza, Marc-Jan Gubbels
PLOS Pathogens.2020; 16(7): e1008599. CrossRef
Expression of 4-Hydroxynonenal (4-HNE) and Heme Oxygenase-1 (HO-1) in the Kidneys of Plasmodium berghei-Infected Mice
Prasit Na-Ek, Chuchard Punsawad, Jianbing Mu
Journal of Tropical Medicine.2020; 2020: 1. CrossRef
Understanding host–parasite relationship: the immune central nervous system microenvironment and its effect on brain infections
Laura Adalid-Peralta, Brenda Sáenz, Gladis Fragoso, Graciela Cárdenas
Parasitology.2018; 145(8): 988. CrossRef
The role of heme-oxygenase-1 in pathogenesis of cerebral malaria in the co-culture model of human brain microvascular endothelial cell and ITG Plasmodium falciparum-infected red blood cells
Pimwan Thongdee, Kesara Na-Bangchang
Asian Pacific Journal of Tropical Medicine.2017; 10(1): 20. CrossRef
PKA and Apicomplexan Parasite Diseases
M. Haidar, G. Ramdani, E. J. Kennedy, G. Langsley
Hormone and Metabolic Research.2017; 49(04): 296. CrossRef
Effect of carbon monoxide on gene expression in cerebrocortical astrocytes: Validation of reference genes for quantitative real-time PCR
Sara R. Oliveira, Helena L.A. Vieira, Carlos B. Duarte
Nitric Oxide.2015; 49: 80. CrossRef
Study on association between genetic polymorphisms of haem oxygenase-1, tumour necrosis factor, cadmium exposure and malaria pathogenicity and severity
Jiraporn Kuesap, Kenji Hirayama, Mihoko Kikuchi, Ronnatrai Ruangweerayut, Kesara Na-Bangchang
Malaria Journal.2010;[Epub] CrossRef