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Original Articles

Trematode metacercariae and adults in cyprinoid fish from Khun Thale Swamp in Surat Thani province, Thailand
Kanda Kamchoo, Jong-Yil Chai
Parasites Hosts Dis 2023;61(2):163-171.
Published online May 23, 2023
DOI: https://doi.org/10.3347/PHD.23030
The present study aimed to determine the infection status of trematode metacercariae and adults in cyprinoid fish from the Khun Thale Swamp in Surat Thani, Southern Thailand, with epidemiologic and faunistic viewpoints. In 2020, 577 fish in 15 species were collected in the summer (February–April) and rainy (September–November) seasons. Fish were individually examined for trematode metacercariae in the whole body and adults in the gastrointestinal tract using a stereomicroscope. Three species of digenetic trematode metacercariae, i.e., Haplorchis taichui, Haplorchoides mehrai, and Centrocestus formosanus, were detected in the muscle, fin, and/or scale of fish. Two species of adult flukes, including Rohdella siamensis and Helostomatis cyprinorum, were collected in the intestines. The prevalence of overall trematode infections was 32.4% (187/577 fish), which was higher in the rainy season (41.4%; 118/285) than in the summer season (23.6%; 69/292). The metacercariae of H. taichui and H. mehrai were detected in 7 fish species each, and those of C. formosanus were found only in Rasbora toneri. The aspidogastrean trematode R. siamensis (adult) was detected in Babonymus gonionotus. A digenean species, H. cyprinorum (adult), was found in Labiobarbus siamensis and Osteochilus vittatus. The present study has first confirmed that the metacercariae of heterophyid flukes, including H. taichui, H. mehrai, and C. formosanus, and adults of R. siamensis (Aspidogastrea) and H. cyprinorum (Digenea) are infected in some species of the cyprinoid fish from the Khun Thale Swamp in Surat Thani, Thailand.

Citations

Citations to this article as recorded by  Crossref logo
  • Influences of parasitic stress on the health condition of African Catfish (Clarias gariepinus): Biochemical and histopathological alterations
    Hadeer Abd El-hak Rashed, Nahla S. El-Shenawy, Nadia A. El-Fahla
    Molecular and Biochemical Parasitology.2025; 262: 111677.     CrossRef
  • 4,134 View
  • 188 Download
  • 2 Web of Science
  • Crossref
Prevalence of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency among Malaria Patients in Southern Thailand: 8 Years Retrospective Study
Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat
Korean J Parasitol 2022;60(1):15-23.
Published online February 23, 2022
DOI: https://doi.org/10.3347/kjp.2022.60.1.15
Erythrocytes deficient in glucose-6-phosphate dehydrogenase (G6PD) is more susceptible to oxidative damage from free radical derived compounds. The hemolysis triggered by oxidative agents such as primaquine (PQ) is used for the radical treatment of hypnozoites of P. vivax. Testing of G6PD screening before malaria treatment is not a common practice in Thailand, which poses patients at risk of hemolysis. This retrospective study aimed to investigate the prevalence of G6PD in malaria patients who live in Southern Thailand. Eight hundred eighty-one malaria patients were collected for 8-year from 2012 to 2019, including 785 (89.1%) of P. vivax, 61 (6.9%) of P. falciparum, 27 (3.1%) of P. knowlesi, and 8 (0.9%) of mixed infections. The DiaPlexC genotyping kit (Asian type) and PCR-RFLP were employed to determine the G6PD variants. The result showed that 5 different types of G6PD variants were identified in 26 cases (2.9%); 12/26 (46.2%) had Mahidol (487G>A) and 11/26 (42.3%) had Viangchan (871G>A) variants, while the rest had Kaiping (1388G>A), Union (1360C>T), and Mediterranean (563C>T) variants. G6PD Songklanagarind (196T>A) variant was not found in the study. Our result did not show a significant difference in the malaria parasite densities in patients between G6PD-deficient and G6PD-normal groups. According to our findings, testing G6PD deficiency and monitoring the potential PQ toxicity in patients who receive PQ are highly recommended.

Citations

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  • Prospective observational study to assess the feasibility and safety of appropriate Plasmodium vivax radical cure with tafenoquine or primaquine after quantitative G6PD testing during pilot implementation in Thailand
    Prayuth Sudathip, Nardlada Khantikul, Aungkana Saejeng, Stephan Duparc, Penny Grewal Daumerie, Caroline Lynch, Elodie Jambert, Saowanee Viboonsanti, Darin Areechokchai, Jerdsuda Kanjanasuwan, Thannika Thong-ard, Panupong Kowsurat, Isabelle Borghini-Fuhrer
    BMJ Global Health.2025; 10(4): e016720.     CrossRef
  • Glucose-6-phosphate dehydrogenase deficiency induced hemolytic anemia and methemoglobinemia: a case report in a 7 -year-old female patient
    Adalgisa Fastuca, Antonio Vergori, Giuseppe Robustelli, Chiara Piccolo, Maria Ragazzo, Maddalena Marinoni, Massimo Agosti
    Italian Journal of Pediatrics.2025;[Epub]     CrossRef
  • Human genetic variations conferring resistance to malaria
    Xiaokun Zhang, Jie Wu, Yunxing Peng, Lan Luo, Lu Zhang, Xi Huang, Guoying Chen, Yirong Li, Haoan Yi
    Journal of Translational Medicine.2025;[Epub]     CrossRef
  • Glucose-6-phosphate dehydrogenase variants in Kachin, Myanmar
    Zin Moon, Ja Moon Aung, Dorene VanBik, Hae Soo Yun, Sanghyun Lee, Sylvatrie-Danne Dinzouna-Boutamba, Zau Ring, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo
    Parasites, Hosts and Diseases.2025; 63(4): 360.     CrossRef
  • Hematological Indicators of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency in Malaria-Infected Individuals
    Donia Zaid Hazem, Esraa Adel Mahmood, Anfal Saleh Mohammed
    Malaysian Journal of Medicine and Health Sciences.2024; 20(1): 46.     CrossRef
  • Single-Drop Blood Detection of Common G6PD Mutations in Thailand Based on Allele-Specific Recombinase Polymerase Amplification with CRISPR-Cas12a
    Punchalee Mungkalasut, Pattaraporn Nimsamer, Poonlarp Cheepsunthorn, Sunchai Payungporn, Chalisa Louicharoen Cheepsunthorn
    ACS Omega.2023; 8(47): 44733.     CrossRef
  • Prevalence of G6PD deficiency and G6PD variants amongst the southern Thai population
    Manit Nuinoon, Rungnapha Krithong, Suputcha Pramtong, Piyawit Sasuk, Chompunuch Ngeaiad, Sathanan Chaimusik, Jiraporn Kanboonma, Orawan Sarakul
    PeerJ.2022; 10: e14208.     CrossRef
  • Cytochrome P450 2D6 (CYP2D6) and glucose-6-phosphate dehydrogenase (G6PD) genetic variations in Thai vivax malaria patients: Implications for 8-aminoquinoline radical cure
    Kamonwan Chamchoy, Sirapapha Sudsumrit, Thanyapit Thita, Srivicha Krudsood, Rapatbhorn Patrapuvich, Usa Boonyuen, Paul O. Mireji
    PLOS Neglected Tropical Diseases.2022; 16(12): e0010986.     CrossRef
  • Plasmodium vivax: the potential obstacles it presents to malaria elimination and eradication
    Kassahun Habtamu, Beyene Petros, Guiyun Yan
    Tropical Diseases, Travel Medicine and Vaccines.2022;[Epub]     CrossRef
  • 7,375 View
  • 224 Download
  • 7 Web of Science
  • Crossref

Case Report

Ocular Sparganosis: The First Report of Spirometra ranarum in Thailand
Wilai Saksirisampant, Chatanun Eamudomkarn, Hyeong-Kyu Jeon, Keeseon S. Eom, Buravej Assavapongpaiboon, Sunisa Sintuwong, Wasee Tulvatana
Korean J Parasitol 2020;58(5):577-581.
Published online October 22, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.5.577
A 22-year-old Thai man from the Northeast region presented with acute eye swelling, itching, and discharge on his left eye. He was suspected of having gnathostomiasis and treated with albendazole and prednisolone for 3 weeks. Nine months later, he was treated with high-dose oral prednisolone for the preliminary and differential diagnoses with thyroid-associated orbitopathy and lymphoma. He had been administered prednisolone intermittently over a few years. Then he developed a painless movable mass at the left upper eyelid and recurrent pseudotumor oculi was suspected. The surgical removal of the mass was performed. A white pseudosegmented worm revealed a definite diagnosis of ocular sparganosis by a plerocercoid larva. Molecular diagnosis of the causative species was made based on the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. Proper technique of extraction and amplification of short fragments DNA from formalin-fixed paraffin-embedded tissue successfully identified parasite species. The result from the sequencing of the PCR-amplified cox1 fragments in this study showed 99.0% sequence homology to Spirometra ranarum. This is the first report of S. ranarum in Thailand.

Citations

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  • Molecular Characterization of Spirometra erinaceieuropaei from Jungle Cat (Felis chaus) in North of Iran
    Mahboobeh Salimi, Meysam Sharifdini, Eshrat Beigom Kia
    Acta Parasitologica.2024; 69(1): 574.     CrossRef
  • Sparganosis
    S. B. Chuelov, A. L. Rossina
    CHILDREN INFECTIONS.2024; 23(1): 44.     CrossRef
  • Sparganosis in the Indian Sub-continent and the Middle East
    Yukifumi Nawa, Mio Tanaka, Masahide Yoshikawa
    Parasites, Hosts and Diseases.2024; 62(3): 263.     CrossRef
  • Medical Parasitology Taxonomy Update, June 2020–June 2022
    Blaine A. Mathison, Richard S. Bradbury, Bobbi S. Pritt, Romney M. Humphries
    Journal of Clinical Microbiology.2023;[Epub]     CrossRef
  • Parasites, champignons et atteintes oculaires
    Hélène Yera, Marie-Fleur Durieux, Dominique Chabasse, Marie-Laure Dardé
    Revue Francophone des Laboratoires.2023; 2023(552): 44.     CrossRef
  • Sparganosis parasitic tumor: Resectability and postoperative outcome, and analysis of cases from an endemic area in Indochina
    Amnuay Kleebayoon, Viroj Wiwanitkit
    Formosan Journal of Surgery.2023; 56(5): 160.     CrossRef
  • An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae
    Blaine A. Mathison, Sarah G. H. Sapp
    ZooKeys.2021; 1069: 1.     CrossRef
  • 5,848 View
  • 123 Download
  • 5 Web of Science
  • Crossref

Original Article

Morphology and Molecular Identification of Echinostoma revolutum and Echinostoma macrorchis in Freshwater Snails and Experimental Hamsters in Upper Northern Thailand
Preeyaporn Butboonchoo, Chalobol Wongsawad, Pheravut Wongsawad, Jong-Yil Chai
Korean J Parasitol 2020;58(5):499-511.
Published online October 22, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.5.499
Echinostome metacercariae were investigated in freshwater snails from 26 districts in 7 provinces of upper northern Thailand. The species identification was carried out based on the morphologies of the metacercariae and adult flukes harvested from experimental hamsters, and on nucleotide sequences of internal transcribed spacer 2 (ITS2) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) genes. Twenty-four out of 26 districts were found to be infected with echinostome metacercariae in freshwater snails with the prevalence of 40.4%. The metacercariae were found in all 6 species of snails, including Filopaludina martensi martensi (21.9%), Filopaludina doliaris (50.8%), F. sumatrensis polygramma (61.3%), Bithynia siamensis siamensis (14.5%), Bithynia pulchella (38.0%), and Anenthome helena (4.9%). The echinostome metacercariae found in these snails were identified as Echinostoma revolutum (37-collar-spined) and Echinostoma macrorchis (45-collar-spined) morphologically and molecularly. The 2-week-old adult flukes of E. revolutum revealed unique features of the cirrus sac extending to middle of the ventral sucker and smooth testes. E. macrorchis adults revealed the cirrus sac close to the right lateral margin of the ventral sucker and 2 large and elliptical testes with slight indentations and pointed posterior end of the posterior testis. The ITS2 and nad1 sequences confirmed the species identification of E. revolutum, and the sequences of E. macrorchis have been deposited for the first time in Gen-Bank. The presence of the life cycle of E. macrorchis is a new record in Thailand and the snail F. doliaris as their second intermediate host seems to be new among the literature.

Citations

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  • Filling the void: Morphological and molecular phylogenetic analyses of helminths assemblage from the Egyptian egret Bubulcus ibis
    Nada A. Ibrahim, Ismail M. Shalaby, Mahi A. Ghobashy, Azza A. Taeleb, Elham A. Elkhawass
    Parasitology International.2025; 104: 102972.     CrossRef
  • The Nuclear Ribosomal Transcription Units of Two Echinostomes and Their Taxonomic Implications for the Family Echinostomatidae
    Yu Cao, Ye Li, Zhong-Yan Gao, Bo-Tao Jiang
    Biology.2025; 14(8): 1101.     CrossRef
  • Prevalence of Foodborne Trematodes in Small Indigenous Fish Species in Local Markets of Phnom Penh, Cambodia
    Domenico Caruso, Lin Kong, Samnang Keo, Sreyni Yoeurn, Samphal Seng
    Vector-Borne and Zoonotic Diseases.2024; 24(3): 141.     CrossRef
  • Morphological and molecular characteristics of Paralecithodendrium longiforme (Digenea: Lecithodendriidae) adults and cercariae from Chinese pipistrelle bats and viviparid snails in Thailand
    Thitichai Arttra, Pheravut Wongsawad, Chalobol Wongsawad, Nattawadee Nantarat, Preeyaporn Butboonchoo, Jong-Yil Chai
    Parasites, Hosts and Diseases.2024; 62(1): 85.     CrossRef
  • Development and utilization of a visual loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) assay for rapid detection of Echinostomatidae metacercaria in edible snail samples
    Wasin Panich, Phonkawin Jaruboonyakorn, Awika Raksaman, Thanawan Tejangkura, Thapana Chontananarth
    International Journal of Food Microbiology.2024; 418: 110732.     CrossRef
  • Genetic diversity and population structure of the helminths intermediate host Anentome helena (von dem Busch, 1847) in northern Thailand
    Nithinan Chomchoei, Chalobol Wongsawad, Preeyaporn Butboonchoo, Nattawadee Nantarat
    Asian Pacific Journal of Tropical Medicine.2024; 17(12): 536.     CrossRef
  • Morphological and DNA analyses reveal cryptic diversity in Anentome wykoffi (Brandt, 1974) (Gastropoda: Nassariidae), with descriptions of two new species from Thailand
    Nithinan Chomchoei, Thierry Backeljau, Piyatida Pimvichai, Ting Hui Ng, Nattawadee Nantarat, Gonzalo Giribet
    Invertebrate Systematics.2023; 37(11): 755.     CrossRef
  • High diversity of trematode metacercariae that parasitize freshwater gastropods in Bangkok, Thailand, and their infective situations, morphologies and phylogenetic relationships
    Pichit Wiroonpan, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
    Parasitology.2022; 149(7): 913.     CrossRef
  • Unwanted ‘hitchhikers’ of ornamental snails: a case report of digeneans transported via the international pet trade
    A. Stanicka, R. Maciaszek, A. Cichy, J. Templin, W. Świderek, E. Żbikowska, A. M. Labecka
    The European Zoological Journal.2022; 89(1): 601.     CrossRef
  • Morphological and molecular characterization of larval trematodes infecting the assassin snail genusAnentomein Thailand
    N. Chomchoei, T. Backeljau, B. Segers, C. Wongsawad, P. Butboonchoo, N. Nantarat
    Journal of Helminthology.2022;[Epub]     CrossRef
  • 10,032 View
  • 234 Download
  • 10 Web of Science
  • Crossref

Case Report

The First Acanthamoeba keratitis Case of Non-Contact Lens Wearer with HIV Infection in Thailand
Napaporn Tananuvat, Natnaree Techajongjintana, Pradya Somboon, Anchalee Wannasan
Korean J Parasitol 2019;57(5):505-511.
Published online October 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.5.505
Acanthamoeba keratitis (AK) is a rare sight-threatening corneal infection, often reporting from contact lens wearers. An asymptomatic human immunodeficiency virus (HIV)-infected Thai male without history of contact lens use complained foreign body sensation at his left eye during motorbike riding. He had neither specific keratitis symptoms nor common drugs responding, which contributed to delayed diagnosis. By corneal re-scraping, Acanthamoeba-like cysts were detected by calcofluor white staining and agar culture. The etiological agent obtained from the culture was molecularly confirmed by Acanthamoeba spp.-specific PCR, followed by DNA sequencing. The results from BLAST and phylogenetic analysis based on the DNA sequences, revealed that the pathogen was Acanthamoeba T4, the major genotype most frequently reported from clinical isolates. The infection was successfully treated with polyhexamethylene biguanide resulting in corneal scar. This appears the first reported AK case from a non-contact lens wearer with HIV infection in Thailand. Although AK is sporadic in developing countries, a role of free-living Acanthamoeba as an opportunistic pathogen should not be neglected. The report would increase awareness of AK, especially in the case presenting unspecific keratitis symptoms without clinical response to empirical antimicrobial therapy.

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  • Detection of Free-Living Amoeba in a tertiary care hospital
    Nairita Choudhuri, Bhaskar Narayan Chaudhuri, Partha Guchhait, Arup Kumar Dawn, Satadal Das
    Microbiologia Medica.2024;[Epub]     CrossRef
  • Diagnosis of Acanthamoeba Keratitis: Past, Present and Future
    Matthew Azzopardi, Yu Jeat Chong, Benjamin Ng, Alberto Recchioni, Abison Logeswaran, Darren S. J. Ting
    Diagnostics.2023; 13(16): 2655.     CrossRef
  • New Frontiers in Acanthamoeba Keratitis Diagnosis and Management
    Omar Shareef, Sana Shareef, Hajirah N. Saeed
    Biology.2023; 12(12): 1489.     CrossRef
  • Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, Iran
    Ehsan Javanmard, Maryam Niyyati, Ali Taghipour, Marziye Fatemi, Hamed Mirjalali, Panagiotis Karanis
    Journal of Water and Health.2022; 20(4): 620.     CrossRef
  • Isolation and identification of potentially pathogenic free-living amoeba in dental-unit water samples
    Hadi Khatoonaki, Kavous Solhjoo, Hassan Rezanezhad, Belal Armand, Amir Abdoli, Ali Taghipour
    Journal of Water and Health.2022; 20(7): 1126.     CrossRef
  • Detection of Acanthamoeba from Acanthamoeba Keratitis Mouse Model Using Acanthamoeba-Specific Antibodies
    Min-Jeong Kim, A-Jeong Ham, A-Young Park, Hae-Jin Sohn, Ho-Joon Shin, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
    Microorganisms.2022; 10(9): 1711.     CrossRef
  • RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
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    Scientific Reports.2021;[Epub]     CrossRef
  • Genotype distribution of Acanthamoeba in keratitis: a systematic review
    Maria Luisa Nunes Diehl, Júlia Paes, Marilise Brittes Rott
    Parasitology Research.2021; 120(9): 3051.     CrossRef
  • Explicating the presentations of Acanthamoeba keratitis with special concern in the COVID-19 pandemic ambient
    Chukwuma Sr Chrysanthus
    International Journal of Clinical Microbiology and Biochemical Technology.2021; 4(1): 010.     CrossRef
  • Effectiveness and Safety of Topical Chlorhexidine and Vitamin E TPGS in the Treatment of Acanthamoeba Keratitis: A Survey on 29 Cases
    Ciro Caruso, Daniela Eletto, Michele Rinaldi, Luigi Pacente, Salvatore Troisi, Francesco Semeraro, Roberto dell’Omo, Ciro Costagliola
    Journal of Clinical Medicine.2020; 9(11): 3775.     CrossRef
  • 18,868 View
  • 132 Download
  • 9 Web of Science
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Original Articles

Molecular Surveillance of Pfkelch13 and Pfmdr1 Mutations in Plasmodium falciparum Isolates from Southern Thailand
Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat
Korean J Parasitol 2019;57(4):369-377.
Published online August 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.4.369
Artemisinin-based combination therapy (ACT) resistance is widespread throughout the Greater Mekong Subregion. This raises concern over the antimalarial treatment in Thailand since it shares borders with Cambodia, Laos, and Myanmar where high ACT failure rates were reported. It is crucial to have information about the spread of ACT resistance for efficient planning and treatment. This study was to identify the molecular markers for antimalarial drug resistance: Pfkelch13 and Pfmdr1 mutations from 5 provinces of southern Thailand, from 2012 to 2017, of which 2 provinces on the Thai- Myanmar border (Chumphon and Ranong), one on Thai-Malaysia border (Yala) and 2 from non-border provinces (Phang Nga and Surat Thani). The results showed that C580Y mutation of Pfkelch13 was found mainly in the province on the Thai-Myanmar border. No mutations in the PfKelch13 gene were found in Surat Thani and Yala. The Pfmdr1 gene isolated from the Thai-Malaysia border was a different pattern from those found in other areas (100% N86Y) whereas wild type strain was present in Phang Nga. Our study indicated that the molecular markers of artemisinin resistance were spread in the provinces bordering along the Thai-Myanmar, and the pattern of Pfmdr1 mutations from the areas along the international border of Thailand differed from those of the non-border provinces. The information of the molecular markers from this study highlighted the recent spread of artemisinin resistant parasites from the endemic area, and the data will be useful for optimizing antimalarial treatment based on regional differences.

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  • Tracking Drug Resistance in Plasmodium falciparum: Genetic Diversity of Key Resistance Markers in Brazilian Malaria Hotspots
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    Wihda Aisarul Azmi, Andita Fitri Mutiara Rizki, Yenny Djuardi, I. Made Artika, Josephine Elizabeth Siregar
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  • Application of loop-mediated isothermal amplification combined with lateral flow assay visualization of Plasmodium falciparum kelch 13 C580Y mutation for artemisinin resistance detection in clinical samples
    Wannida Sanmoung, Nongyao Sawangjaroen, Suwannee Jitueakul, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat, Mallika Imwong
    Acta Tropica.2023; 246: 106998.     CrossRef
  • Prevalence of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency among Malaria Patients in Southern Thailand: 8 Years Retrospective Study
    Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat
    The Korean Journal of Parasitology.2022; 60(1): 15.     CrossRef
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    European Journal of Medicinal Chemistry.2021; 210: 112955.     CrossRef
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    Yacoba V.T. Minnow, Rajesh K. Harijan, Vern L. Schramm
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  • A LAMP-SNP Assay Detecting C580Y Mutation in Pfkelch13 Gene from Clinically Dried Blood Spot Samples
    Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat
    The Korean Journal of Parasitology.2021; 59(1): 15.     CrossRef
  • New insights into the spread of resistance to artemisinin and its analogues
    Noreen Noreen, Asad Ullah, Syed Muhammad Salman, Yahia Mabkhot, Abdulrhman Alsayari, Syed Lal Badshah
    Journal of Global Antimicrobial Resistance.2021; 27: 142.     CrossRef
  • Efficacy and safety of artemether-lumefantrine and dihydroartemisinin-piperaquine for the treatment of uncomplicated Plasmodium falciparum malaria and prevalence of molecular markers associated with artemisinin and partner drug resistance in Uganda
    Chris Ebong, Asadu Sserwanga, Jane Frances Namuganga, James Kapisi, Arthur Mpimbaza, Samuel Gonahasa, Victor Asua, Sam Gudoi, Ruth Kigozi, James Tibenderana, John Bosco Bwanika, Agaba Bosco, Denis Rubahika, Daniel Kyabayinze, Jimmy Opigo, Damian Rutazana,
    Malaria Journal.2021;[Epub]     CrossRef
  • 7,534 View
  • 221 Download
  • 13 Web of Science
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Morphological and Molecular Identification of Stellantchasmus dermogenysi n. sp. (Digenea: Heterophyidae) in Thailand
Chalobol Wongsawad, Nattawadee Nantarat, Pheravut Wongsawad, Preeyaporn Butboonchoo, Jong-Yil Chai
Korean J Parasitol 2019;57(3):257-264.
Published online June 30, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.3.257
We tried a series of morphological and molecular approaches to identify a new species of Stellantchasmus (Digenea: Heterophyidae) originating from the wrestling half-beaked fish, Dermogenys pusillus of Thailand. Adult worm samples of the new species were recovered from hamsters experimentally infected with the metacercariae from D. pusillus in Thailand. Two isolates (Thai and Korean) of Stellantchasmus falcatus were used as comparative control groups. Worm samples of 3 Stellantchasmus groups were morphologically observed and molecularly analyzed with the mitochondrial cytochrome c oxidase 1 gene. The morphological characteristics of S. dermogenysi n. sp. are similar to S. falcatus originating from brackish water fish, but minor difference was noted including the absence of the prepharynx, position of the ovary near the ceca end, smaller body size, and shorter esophageal length. A phylogenetic tree derived from neighborjoining and maximum-likelihood methods suggests that S. dermogenysi n. sp. is separated from S. falcatus supported by high bootstrap values. The relative divergences persist between these host-specific trematodes, which we suggest should be recognized as 2 distinct species. Comparisons of S. dermogenysi n. sp. with S. falcatus isolated from mullets in Thailand and Korea indicate a genetic divergence of mitochondrial DNA of 19.4% and 21.7%, respectively. By the present study, a new species, Stellantchasmus dermogenysi n. sp. (Digenea: Heterophyidae), is proposed in Thailand based on molecular evidences, in addition to minor morphological differences between S. falcatus and the new species.

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  • Morphological and molecular characterization of larval trematodes infecting the assassin snail genusAnentomein Thailand
    N. Chomchoei, T. Backeljau, B. Segers, C. Wongsawad, P. Butboonchoo, N. Nantarat
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  • Infections of Digenetic Trematode Metacercariae in Wrestling Halfbeak, Dermogenys pusilla from Bangkok Metropolitan Region in Thailand
    Laddawan Patarwut, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
    The Korean Journal of Parasitology.2020; 58(1): 27.     CrossRef
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Prevalence of Intestinal Parasites and Associated Risk Factors for Infection among Rural Communities of Chachoengsao Province, Thailand
Pisit Suntaravitun, Amornrat Dokmaikaw
Korean J Parasitol 2018;56(1):33-39.
Published online February 28, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.1.33
Intestinal parasitic infections remain a major public health problem in many parts of Thailand, particularly in rural areas. This study aimed to determine the prevalence of intestinal parasitic infections and associated risk factors among the people living in Huai Sai sub-district, Bang Khla district, Chachoengsao Province, central Thailand. A crosssectional survey was carried out from June 2017 to August 2017 which included a total of 224 participants. Stool samples were examined using a simple direct smear and formalin ethyl acetate concentration technique. Association between risk factors and intestinal parasitic infections was assessed using multivariate logistic regression analysis. The overall prevalence of intestinal parasitic infections was 16.1%. Soil-transmitted helminth infections (14.3%) were more common than protozoan infections (1.8%). The most common intestinal parasites were hookworms (6.7%) followed by Strongyloides stercoralis, (5.0%), Ascaris lumbricoides (1.3%) and Trichuris trichiura (1.3%). Entamoeba histolytica/dispar (1.0%), Giardia intestinalis (0.4%), and Blastocystis hominis (0.4%) were the protozoans identified. A high prevalence of infections was found in male participants of ≥ 40 years who raised dogs in the households and did not wear boots while working fields. Multivariate analysis showed a significant association of intestinal parasitic infections with gender with the adjusted odds ratio (OR) of 2.4 and 95% confidence interval (CI) of 1.1-5.2 (P = 0.020). The results showed a high prevalence of soil transmitted helminth infections among adults in rural communities which were particularly apparent regarding the skin-penetrating species of nematodes. A greater focus on intervention is required by improving sanitation and personal hygiene to prevent the spread of intestinal parasitic infections.

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Population Genetics of Plasmodium vivax in Four High Malaria Endemic Areas in Thailand
Kanungnit Congpuong, Ratawan Ubalee
Korean J Parasitol 2017;55(5):465-472.
Published online October 31, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.5.465
Recent trends of malaria in Thailand illustrate an increasing proportion of Plasmodium vivax, indicating the importance of P. vivax as a major causative agent of malaria. P. vivax malaria is usually considered a benign disease so the knowledge of this parasite has been limited, especially the genetic diversity and genetic structure of isolates from different endemic areas. The aim of this study was to examine the population genetics and structure of P. vivax isolates from 4 provinces with different malaria endemic settings in Thailand using 6 microsatellite markers. Total 234 blood samples from P. vivax mono-infected patients were collected. Strong genetic diversity was observed across all study sites; the expected heterozygosity values ranged from 0.5871 to 0.9033. Genetic variability in this study divided P. vivax population into 3 clusters; first was P. vivax isolates from Mae Hong Son and Kanchanaburi Provinces located on the western part of Thailand; second, Yala isolates from the south; and third, Chanthaburi isolates from the east. P. vivax isolates from patients having parasite clearance time (PCT) longer than 24 hr after the first dose of chloroquine treatment had higher diversity when compared with those having PCT within 24 hr. This study revealed a clear evidence of different population structure of P. vivax from different malaria endemic areas of Thailand. The findings provide beneficial information to malaria control programme as it is a useful tool to track the source of infections and current malaria control efforts.

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  • Low Genetic Diversity of Plasmodium vivax Circumsporozoite Surface Protein in Clinical Isolates from Southern Thailand
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    Yubing Hu, Yuling Li, Awtum M. Brashear, Weilin Zeng, Zifang Wu, Lin Wang, Haichao Wei, Myat Thu Soe, Pyae Linn Aung, Jetsumon Sattabongkot, Myat Phone Kyaw, Zhaoqing Yang, Yan Zhao, Liwang Cui, Yaming Cao, Karin Kirchgatter
    PLOS Neglected Tropical Diseases.2024; 18(7): e0012299.     CrossRef
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    Musab M. Ali Albsheer, Alfred Hubbard, Cheikh Cambel Dieng, Eyoab Iyasu Gebremeskel, Safaa Ahmed, Virginie Rougeron, Muntaser E. Ibrahim, Eugenia Lo, Muzamil M. Abdel Hamid
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  • Comparison of Vector Trapping Methods for Outdoor Biting Malaria Vector Surveillance in Thailand and Vietnam
    Ratchadawan Ngoenklan, Tran Thanh Duong, Vu Duc Chinh, Nguyen Quang Thieu, Jeffrey Hii, Michael J Bangs, Boonserm Aum-Aung, Wannapa Suwonkerd, Theeraphap Chareonviriyaphap, Nobuko Tuno
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Case Report

Forensically Important Blow Flies Chrysomya pinguis, C. villeneuvi, and Lucilia porphyrina (Diptera: Calliphoridae) in a Case of Human Remains in Thailand
Tawatchai Monum, Kabkaew L. Sukontason, Pongruk Sribanditmongkol, Kom Sukontason, Chutharat Samerjai, Kwankamol Limsopatham, Suttida Suwannayod, Tunwadee Klong-klaew, Anchalee Wannasan
Korean J Parasitol 2017;55(1):71-76.
Published online February 28, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.1.71
This is the first study to report Chrysomya pinguis (Walker) and Lucilia porphyrina (Walker) (Diptera: Calliphoridae) as forensically important blow fly species from human cadavers in Thailand, in addition to Chrysomya villeneuvi (Patton) already known in Thailand. In 2016, a fully decomposed body of an unknown adult male was discovered in a high mountainous forest during winter in Chiang Mai province. The remains were infested heavily with thousands of blow fly larvae feeding simultaneously on them. Morphological identification of adults reared from the larvae, and molecular analysis based on sequencing of 1,247 bp partial mitochondrial cytochrome c oxidase subunit 1 gene (CO1) of the larvae and puparia, confirmed the above mentioned 3 species. The approving forensic fly evidence by molecular approach was described for the first time in Thailand. Moreover, neighbor-joining phylogenetic analysis of the CO1 was performed to compare the relatedness of the species, thereby affirming the accuracy of identification. As species of entomofauna varies among cases in different geographic and climatic circumstances, C. pinguis and L. porphyrina were added to the list of Thai forensic entomology caseworks, including colonizers of human remains in open, high mountainous areas during winter. Further research should focus on these 3 species, for which no developmental data are currently available.

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  • Origins and Diversification of Myiasis Across Blowflies
    Gisele Antoniazzi Cardoso, Vanessa A. S. Cunha, Bruno C. Genevcius, Tais Madeira‐Ott, Bárbara Maria de Andrade Costa, Daniela Munhoz Rossoni, Patricia Jacqueline Thyssen, Tatiana Teixeira Torres
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    Nikolas P. Johnston, Nathan J. Butterworth, Kelly A. Meiklejohn, Andrzej Grzywacz, Thomas Pape, Krzysztof Szpila, James F. Wallman
    Austral Entomology.2025;[Epub]     CrossRef
  • Initial insights into using outline-based geometric morphometrics of the larval cephaloskeleton to identify forensically important calliphorids in Thailand
    Wanida Kanta, Sangob Sanit, Kabkaew L Sukontason, Jean-Pierre Dujardin, Sebastien Dujardin, Kwankamol Limsopatham, Ian Dadour
    Journal of Medical Entomology.2025;[Epub]     CrossRef
  • Geometry of posterior larval spiracles to identify medically and forensically important calliphorids in Thailand
    Wanida Kanta, Kwankamol Limsopatham, Kabkaew L. Sukontason, Kom Sukontason, Jean-Pierre Dujardin, Sebastien Dujardin, Sangob Sanit
    Acta Tropica.2024; 252: 107126.     CrossRef
  • The Study of Forensically Important Insects Recovered from Human Corpses in Taiwan
    Wei-Lun Yan, Chiou-Herr Yang, Siew Hwa Tan, Chung-Yen Pai, Kan-Kun Li, Chen-Chou Chung
    Insects.2023; 14(4): 346.     CrossRef
  • Wing morphometrics as a tool for the identification of forensic important Lucilia spp. (Diptera: Calliphoridae)
    Kwankamol Limsopatham, Chatnaphat Tanajitaree, Sangob Sanit, Kom Sukontason, Pradya Somboon, Jens Amendt, Nina Feddern, Kabkaew L. Sukontason
    Acta Tropica.2022; 226: 106242.     CrossRef
  • Mitogenome-wise codon usage pattern from comparative analysis of the first mitogenome of Blepharipa sp. (Muga uzifly) with other Oestroid flies
    Debajyoti Kabiraj, Hasnahana Chetia, Adhiraj Nath, Pragya Sharma, Ponnala Vimal Mosahari, Deepika Singh, Palash Dutta, Kartik Neog, Utpal Bora
    Scientific Reports.2022;[Epub]     CrossRef
  • Chrysomya pinguis (Walker) (Diptera: Calliphoridae), blow fly of forensic importance: A review of bionomics and forensic entomology appraisal
    Kabkaew L. Sukontason, Sangob Sanit, Kwankamol Limsopatham, Anchalee Wannasan, Pradya Somboon, Kom Sukontason
    Acta Tropica.2022; 232: 106506.     CrossRef
  • ORIGIN OF MYIASIS
    Alen J Salerian
    INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH.2022; : 39.     CrossRef
  • Stay cool or get hot? An applied primer for using temperature in forensic entomological case work
    Lena Lutz, Jens Amendt
    Science & Justice.2020; 60(5): 415.     CrossRef
  • Review of Molecular Identification Techniques for Forensically Important Diptera
    M Denise Gemmellaro, George C Hamilton, Jessica L Ware
    Journal of Medical Entomology.2019; 56(4): 887.     CrossRef
  • Gene expression during the intra-puparial stage of Chrysomya megacephala: Implications for postmortem interval estimation
    Yu Wang, Zhiya Gu, Liangliang Li, Jiangfeng Wang
    Journal of Asia-Pacific Entomology.2019; 22(3): 841.     CrossRef
  • Temperature-dependent development of the blow fly Chrysomya pinguis and its significance in estimating postmortem interval
    Yingna Zhang, Yu Wang, Jian Sun, Guoliang Hu, Man Wang, Jens Amendt, Jiangfeng Wang
    Royal Society Open Science.2019; 6(9): 190003.     CrossRef
  • Chrysomya chani Kurahashi (Diptera: Calliphoridae), a blow fly species of forensic importance: morphological characters of the third larval instar and a case report from Thailand
    Kabkaew L. Sukontason, Tanin Bhoopat, Anchalee Wannasan, Narin Sontigun, Sangob Sanit, Jens Amendt, Chutharat Samerjai, Kom Sukontason
    Forensic Sciences Research.2018; 3(1): 83.     CrossRef
  • Predicting Geographic Distribution of Forensically Significant Blow Flies of Subfamily Chrysomyinae (Diptera: Calliphoridae) in Northern Thailand
    Tunwadee Klong-klaew, Ratchadawan Ngoen-klan, Kittikhun Moophayak, Kom Sukontason, Kim N. Irvine, Jeffery K. Tomberlin, Pradya Somboon, Theeraphap Chareonviriyaphap, Hiromu Kurahashi, Kabkaew L. Sukontason
    Insects.2018; 9(3): 106.     CrossRef
  • Molecular Analysis of Forensically Important Blow Flies in Thailand
    Narin Sontigun, Kabkaew L. Sukontason, Jens Amendt, Barbara K. Zajac, Richard Zehner, Kom Sukontason, Theeraphap Chareonviriyaphap, Anchalee Wannasan
    Insects.2018; 9(4): 159.     CrossRef
  • Spatial Distribution of Forensically Significant Blow Flies in Subfamily Luciliinae (Diptera: Calliphoridae), Chiang Mai Province, Northern Thailand: Observations and Modeling Using GIS
    Tunwadee Klong-klaew, Ratchadawan Ngoen-klan, Kittikhun Moophayak, Kom Sukontason, Kim N. Irvine, Jeffery K. Tomberlin, Hiromu Kurahashi, Theeraphap Chareonviriyaphap, Pradya Somboon, Kabkaew L. Sukontason
    Insects.2018; 9(4): 181.     CrossRef
  • Morphology of immature stages of blow fly, Lucilia sinensis Aubertin (Diptera: Calliphoridae), a potential species of forensic importance
    Sangob Sanit, Kom Sukontason, Hiromu Kurahashi, Jeffery K. Tomberlin, Anchalee Wannasan, Rungroj Kraisittipanit, Kabkaew L. Sukontason
    Acta Tropica.2017; 176: 395.     CrossRef
  • 14,073 View
  • 199 Download
  • 19 Web of Science
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Original Articles

Morphological Characteristics and Phylogenetic Trends of Trematode Cercariae in Freshwater Snails from Nakhon Nayok Province, Thailand
Thapana Chontananarth, Thanawan Tejangkura, Napat Wetchasart, Cherdchay Chimburut
Korean J Parasitol 2017;55(1):47-54.
Published online February 28, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.1.47
The prevalence of cercarial infection in freshwater snails and their evolutionary trends were studied in Nakhon Nayok province, Thailand. A total of 2,869 individual snails were examined for parasitic infections. The results showed that 12 snail species were found to host larval stages of trematodes with an overall prevalence of 4.7%. The infected specimens included 7 types at the cercarial stage; cercariae, megalurous cercariae, echinostome cercariae, furcocercous cercariae, parapleurolophocercous cercariae, virgulate cercariae, and xiphidiocercariae. Regarding molecular identification, ITS2 sequence data of each larval trematode were analyzed, and a dendrogram was constructed using the neighbor-joining method with 10,000 replicates. The dendrogram was separated into 6 clades (order/family), including Echinostomatida/Echinostomatidae, Echinostomatida/Philophthalmidae, Opisthorchiida/Heterophyidae, Plagiorchiida/Prosthogonimidae, Plagiorchiida/Lecithodendriidae, and Strigeatida/Cyathocotylidae. These findings were used to confirm morphological characteristics and evolutionary trends of each type of cercariae discovered in Nakhon Nayok province. Furthermore, this investigation confirmed that the ITS2 data of cercariae could be used to study on phylogenetic relationships or to determine classification of this species at order and/or family level when possible.

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Molecular Phylogenetics of Centrocestus formosanus (Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand
Chalobol Wongsawad, Pheravut Wongsawad, Kom Sukontason, Worawit Maneepitaksanti, Nattawadee Nantarat
Korean J Parasitol 2017;55(1):31-37.
Published online February 28, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.1.31
This study aimed to investigate the morphology and reconstruct the phylogenetic relationships of Centrocestus formosanus originating from 5 species of freshwater fish, i.e., Esomus metallicus, Puntius brevis, Anabas testudineus, Parambassis siamensis, and Carassius auratus, in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adult C. formosanus from day 5 after infection in chicks. C. formosanus originated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those from C. auratus which revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability of C. formosanus also occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity of C. formosanus with 34 circumoral spines originating from C. auratus in the future.

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  • Loop‐Mediated Isothermal Amplification Combined With Lateral‐Flow Dipstick for Detection of Centrocestus formosanus in Ornamental Fish
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    Phonkawin Jaruboonyakorn, Thanawan Tejangkura, Thapana Chontananarth
    Aquaculture.2022; 548: 737660.     CrossRef
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    Aquaculture.2021; 541: 736791.     CrossRef
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    Alaa Eldin Eissa, Marwa M. Attia, Mamdouh Y. Elgendy, Gehad A. Ismail, Nader M. Sabry, Abdelbary Prince, Mahmoud A. Mahmoud, Ghada O. El-Demerdash, Mohamed Abdelsalam, Hassan I.M. Derwa
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  • Molecular classification of rumen fluke eggs in fecal specimens from Suphanburi Province, Thailand, based on cytochrome C oxidase subunit 1
    Sothorn Anucherngchai, Thapana Chontananarth, Thanawan Tejangkura, Chalobol Wongsawad
    Veterinary Parasitology: Regional Studies and Reports.2020; 20: 100382.     CrossRef
  • Infections of Digenetic Trematode Metacercariae in Wrestling Halfbeak, Dermogenys pusilla from Bangkok Metropolitan Region in Thailand
    Laddawan Patarwut, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
    The Korean Journal of Parasitology.2020; 58(1): 27.     CrossRef
  • Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio) in Italy: A Window to a New Globalization-Derived Invasive Microorganism
    Antonino Pace, Ludovico Dipineto, Serena Aceto, Maria Concetta Censullo, Maria Carmen Valoroso, Lorena Varriale, Laura Rinaldi, Lucia Francesca Menna, Alessandro Fioretti, Luca Borrelli
    Animals.2020; 10(3): 456.     CrossRef
  • The life cycle of a zoonotic parasite reassessed: Experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae)
    Hudson A. Pinto, Nicole Q. Gonçalves, Danimar López-Hernandez, Eduardo A. Pulido-Murillo, Alan L. Melo, Petr Heneberg
    PLOS ONE.2018; 13(4): e0194161.     CrossRef
  • Prevalence of Centrocestus formosanus Metacercariae in Ornamental Fish from Chiang Mai, Thailand, with Molecular Approach Using ITS2
    Atcharaphan Wanlop, Chalobol Wongsawad, Pongphol Prattapong, Pheravut Wongsawad, Thapana Chontananarth, Jong-Yil Chai
    The Korean Journal of Parasitology.2017; 55(4): 445.     CrossRef
  • 9,848 View
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  • 14 Web of Science
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Morphology and Molecular Phylogeny of Raillietina spp. (Cestoda: Cyclophyllidea: Davaineidae) from Domestic Chickens in Thailand
Preeyaporn Butboonchoo, Chalobol Wongsawad, Amnat Rojanapaibul, Jong-Yil Chai
Korean J Parasitol 2016;54(6):777-786.
Published online December 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.6.777
Raillietina species are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification of Raillietina has been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies of Raillietina spp. Four Raillietina species, including R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded from Raillietina sp. appeared to be monophyletic. The query sequences of R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genus Raillietina in Thailand.

Citations

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    Wasin Panich, Thanawan Tejangkura, Thapana Chontananarth
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    Journal of Veterinary Medical Science.2024; 86(2): 224.     CrossRef
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    Wasin Panich, Thanawan Tejangkura, Thapana Chontananarth
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    Wasin Panich, Thanawan Tejangkura, Thapana Chontananarth
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  • Molecular detection of three intestinal cestode species (Raillietina echinobothrida, R. tetragona, R. cesticillus) from poultry in Thailand
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    Saleh Al Quraishy, Rewaida Abdel-Gaber, Reem Alajmi, Mohamed A. Dkhil, Munirah Al Jawher, Kareem Morsy
    Parasitology International.2019; 71: 59.     CrossRef
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  • 640 Download
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Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand
Attakorn Palasuwan, Duangdao Palasuwan, Aongart Mahittikorn, Rachatawan Chiabchalard, Valery Combes, Supaluk Popruk
Korean J Parasitol 2016;54(4):455-460.
Published online August 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.4.455
Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of Blastocystis in villagers living along the Chao Phraya River, Ayutthaya Province, Thailand, and to assess the risk of zoonotic infection. In total, 220 stool samples were collected, and DNA was extracted. PCR and sequencing were performed with primers targeting the small-subunit ribosomal RNA (SSU rRNA) genes. Blastocystis was present in 5.9% (13/220) of samples, and ST3 (5.0%; 11/220) was the predominant subtype, followed by ST2 (0.45%; 1/220) and ST6 (0.45%; 1/220). Phylogenetic trees were constructed with the maximum-likelihood method based on the Hasegawa?Kishino?Yano + G + I model, neighbor-joining, and maximum parsimony methods. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. All the sequences of the Blastocystis-positive samples (KU051524?KU051536) were closely related to those from animals (pig, cattle, and chicken), indicating a zoonotic risk. Therefore, the villagers require proper health education, especially regarding the prevention of parasitic infection, to improve their personal hygiene and community health. Further studies are required to investigate the Blastocystis STs in the animals living in these villages.

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  • Assessing Zoonotic Risks of Blastocystis Infection in Singapore
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    Pathogens.2025; 14(8): 773.     CrossRef
  • Prevalence and subtype distribution of Blastocystis infections among community participants in Thailand: a systematic review and meta-analysis
    Manas Kotepui, Supaluk Popruk, Kwuntida Uthaisar Kotepui, Frederick Ramirez Masangkay, Kinley Wangdi, Aongart Mahittikorn, Christen Rune Stensvold
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    Amara Yowang, Anastasios D. Tsaousis, Tawatchai Chumphonsuk, Nontaphat Thongsin, Niwed Kullawong, Siam Popluechai, Eleni Gentekaki
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    Infection, Genetics and Evolution.2018; 65: 107.     CrossRef
  • Blastocystis subtype 5: Predominant subtype on pig farms, Thailand
    Ai-rada Pintong, Suparat Sunyanusin, Rapeepan Prasertbun, Aongart Mahittikorn, Hirotake Mori, Tanasak Changbunjong, Chalit Komalamisra, Yaowalark Sukthana, Supaluk Popruk
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Molecular Identification of Cryptosporidium Species from Pet Snakes in Thailand
Benjarat Yimming, Khampee Pattanatanang, Pornchai Sanyathitiseree, Tawin Inpankaew, Ketsarin Kamyingkird, Nongnuch Pinyopanuwat, Wissanuwat Chimnoi, Jumnongjit Phasuk
Korean J Parasitol 2016;54(4):423-429.
Published online August 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.4.423
Cryptosporidium is an important pathogen causing gastrointestinal disease in snakes and is distributed worldwide. The main
objective
s of this study were to detect and identify Cryptosporidium species in captive snakes from exotic pet shops and snake farms in Thailand. In total, 165 fecal samples were examined from 8 snake species, boa constrictor (Boa constrictor constrictor), corn snake (Elaphe guttata), ball python (Python regius), milk snake (Lampropeltis triangulum), king snake (Lampropeltis getula), rock python (Python sebae), rainbow boa (Epicrates cenchria), and carpet python (Morelia spilota). Cryptosporidium oocysts were examined using the dimethyl sulfoxide (DMSO)-modified acid-fast staining and a molecular method based on nested-PCR, PCR-RFLP analysis, and sequencing amplification of the SSU rRNA gene. DMSO-modified acid-fast staining revealed the presence of Cryptosporidium oocysts in 12 out of 165 (7.3%) samples, whereas PCR produced positive results in 40 (24.2%) samples. Molecular characterization indicated the presence of Cryptosporidium parvum (mouse genotype) as the most common species in 24 samples (60%) from 5 species of snake followed by Cryptosporidium serpentis in 9 samples (22.5%) from 2 species of snake and Cryptosporidium muris in 3 samples (7.5%) from P. regius.

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  • Cryptosporidium spp. in reptiles: Detection challenges, molecular characterization and zoonotic risk
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  • Minimal zoonotic risk of cryptosporidiosis and giardiasis from frogs and reptiles
    Siobhon Egan, Amanda D. Barbosa, Yaoyu Feng, Lihua Xiao, Una Ryan
    European Journal of Protistology.2024; 93: 126066.     CrossRef
  • Cryptosporidium spp. in captive snakes from 26 provinces in China: Prevalence, molecular characterization, and symptoms
    Yilei Zhang, Zhenxiao Lu, Lingru He, Guodong Xiao, Lijie Tian, Jiawei Zhu, Tian Liu, Qiangxin Ou, Haibo Chen, Yew Hwong, Yangjun Kang, Qianming Xu, Qingxun Zhang, Congshan Yang
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  • Investigation of Parasitic Infection in Crocodile Lizards (Shinisaurus crocodilurus) Using High-Throughput Sequencing
    Yongru Zeng, Yi Xiong, Chunsheng Yang, Nan He, Jiasong He, Wenxian Luo, Yaohuan Chen, Xiaochen Zeng, Zhengjun Wu
    Animals.2022; 12(20): 2726.     CrossRef
  • Prevalence and Molecular Characterization of Cryptosporidium serpentis in Captive Snakes in China
    Yijun Chai, Haifeng Liu, Lei Deng, Bo Bi, Jingxin Yao, Xingtao Yang, Zhijun Zhong, Hualin Fu, Liuhong Shen, Ziyao Zhou, Yi Geng, Guangneng Peng
    Journal of Parasitology.2021;[Epub]     CrossRef
  • Evaluation of Paromomycin Treatment for Cryptosporidium serpentis Infection in Eastern Indigo Snakes (Drymarchon couperi)
    James E. Bogan, Michelle Hoffman, Falicia Dickerson, Mark A. Mitchell, Michael M. Garner, April Childress, James F.X. Wellehan
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    Rie Kubota, Toshihiro Tokiwa, Katsuki Matsubara, Minoru Okamoto, Kazunori Ike
    International Journal for Parasitology: Parasites and Wildlife.2020; 11: 83.     CrossRef
  • The first genetic assessment of wild and farmed ball pythons (Reptilia, Serpentes, Pythonidae) in southern Togo
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  • Molecular identification of Cryptosporidium spp. in pet snakes in Beijing, China
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    Parasitology Research.2020; 119(9): 3119.     CrossRef
  • Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China
    Xiao Xiao, Rui Qi, Hui-Ju Han, Jian-Wei Liu, Xiang-Rong Qin, Li-Zhu Fang, Chuan-Min Zhou, Xiao-Qing Gong, Si-Cong Lei, Xue-Jie Yu
    International Journal for Parasitology: Parasites and Wildlife.2019; 10: 274.     CrossRef
  • Gastric Cryptosporidiosis in Snakes, a Review
    James E. Bogan
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Plasmodium falciparum Genotype Diversity in Artemisinin Derivatives Treatment Failure Patients along the Thai-Myanmar Border
Kanungnit Congpuong, Thirasak Hoonchaiyapoom, Kornnarin Inorn
Korean J Parasitol 2014;52(6):631-637.
Published online December 23, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.6.631

Genetic characteristics of Plasmodium falciparum may play a role in the treatment outcome of malaria infection. We have studied the association between diversity at the merozoite surface protein-1 (msp-1), msp-2, and glutamate-rich protein (glurp) loci and the treatment outcome of uncomplicated falciparum malaria patients along the Thai-Myanmar border who were treated with artemisinin derivatives combination therapy. P. falciparum isolates were collected prior to treatment from 3 groups of patients; 50 cases of treatment failures, 50 recrudescences, and 56 successful treatments. Genotyping of the 3 polymorphic markers was analyzed by nested PCR. The distribution of msp-1 alleles was significantly different among the 3 groups of patients but not the msp-2 and glurp alleles. The allelic frequencies of K1 and MAD20 alleles of msp1 gene were higher while RO33 allele was significantly lower in the successful treatment group. Treatment failure samples had a higher median number of alleles as compared to the successful treatment group. Specific genotypes of msp-1, msp-2, and glurp were significantly associated with the treatment outcomes. Three allelic size variants were significantly higher among the isolates from the treatment failure groups, i.e., K1270-290, 3D7610-630, G650-690, while 2 variants, K1150-170, and 3D7670-690 were significantly lower. In conclusion, the present study reports the differences in multiplicity of infection and distribution of specific alleles of msp-1, msp-2, and glurp genes in P. falciparum isolates obtained from treatment failure and successful treatment patients following artemisinin derivatives combination therapy.

Citations

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  • Size and sequence polymorphisms in the glutamate-rich protein gene of the human malaria parasite Plasmodium falciparum in Thailand
    Sittiporn Pattaradilokrat, Chawinya Trakoolsoontorn, Phumin Simpalipan, Natapot Warrit, Morakot Kaewthamasorn, Pongchai Harnyuttanakorn
    Parasites & Vectors.2018;[Epub]     CrossRef
  • Genome-wide association analysis identifies genetic loci associated with resistance to multiple antimalarials in Plasmodium falciparum from China-Myanmar border
    Zenglei Wang, Mynthia Cabrera, Jingyun Yang, Lili Yuan, Bhavna Gupta, Xiaoying Liang, Karen Kemirembe, Sony Shrestha, Awtum Brashear, Xiaolian Li, Stephen F. Porcella, Jun Miao, Zhaoqing Yang, Xin-zhuan Su, Liwang Cui
    Scientific Reports.2016;[Epub]     CrossRef
  • 8,547 View
  • 89 Download
  • 2 Web of Science
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Brief Communications

Evolution of Genetic Polymorphisms of Plasmodium falciparum Merozoite Surface Protein (PfMSP) in Thailand
Jiraporn Kuesap, Wanna Chaijaroenkul, Kanchanok Ketprathum, Puntanat Tattiyapong, Kesara Na-Bangchang
Korean J Parasitol 2014;52(1):105-109.
Published online February 19, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.1.105

Plasmodium falciparum malaria is a major public health problem in Thailand due to the emergence of multidrug resistance. The understanding of genetic diversity of malaria parasites is essential for developing effective drugs and vaccines. The genetic diversity of the merozoite surface protein-1 (PfMSP-1) and merozoite surface protein-2 (PfMSP-2) genes was investigated in a total of 145 P. falciparum isolates collected from Mae Sot District, Tak Province, Thailand during 3 different periods (1997-1999, 2005-2007, and 2009-2010). Analysis of genetic polymorphisms was performed to track the evolution of genetic change of P. falciparum using PCR. Both individual genes and their combination patterns showed marked genetic diversity during the 3 study periods. The results strongly support that P. falciparum isolates in Thailand are markedly diverse and patterns changed with time. These 2 polymorphic genes could be used as molecular markers to detect multiple clone infections and differentiate recrudescence from reinfection in P. falciparum isolates in Thailand.

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  • Immunization with PfGBP130 generates antibodies that inhibit RBC invasion by P. falciparum parasites
    Yannick Johnson, Ahmad Rushdi Shakri, Sunthorn Pond-Tor, Anup Jnawali, Tanbir Najrana, Haiwei Wu, Jhasketan Badhai, Mohamad-Gabriel Alameh, Drew Weissman, Edward Kabyemela, Patrick Duffy, Michal Fried, Jonathan Kurtis, Dipak Kumar Raj
    Frontiers in Immunology.2024;[Epub]     CrossRef
  • Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
    Mary A. Oboh, Tolla Ndiaye, Khadim Diongue, Yaye D. Ndiaye, Mouhamad Sy, Awa B. Deme, Mamadou A. Diallo, Mamadou S. Yade, Sarah K. Volkman, Aida S. Badiane, Alfred Amambua-Ngwa, Daouda Ndiaye
    Malaria Journal.2021;[Epub]     CrossRef
  • Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region
    Cang-Lin Zhang, Hong-Ning Zhou, Quan Liu, Ya-Ming Yang
    Malaria Journal.2019;[Epub]     CrossRef
  • 9,219 View
  • 87 Download
  • 6 Web of Science
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High Prevalence of Haplorchiasis in Nan and Lampang Provinces, Thailand, Proven by Adult Worm Recovery from Suspected Opisthorchiasis Cases
Adulsak Wijit, Nimit Morakote, Jaewwaew Klinchid
Korean J Parasitol 2013;51(6):767-769.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.767

Opisthorchiasis, a risk factor for cholangiocarcinoma in humans, is of public health importance in Thailand. The Annual Surveillance Reports from Nan and Lampang Provinces, Thailand, for the year 2011 showed an opisthorchiasis prevalence of over 70% by recovery of eggs in the feces. This study investigated whether most cases are actually due to minute intestinal flukes (MIF) rather than Opisthorchis viverrini, as the eggs of both can hardly be differentiated by morphology. Fifty and 100 cases from residents in Nan and Lampang, respectively, had stools positive for eggs initially assumed to be those of O. viverrini. Each patient was given praziquantel at 40 mg/kg in a single dose. After 2 hr, 30-45 ml of the purgative magnesium sulfate was given, and stools were collected up to 4 times sequentially. The stools were examined for adult worms by simple sedimentation. It was found that 39 of 50 cases (78.0%) from Nan Province had Haplorchis taichui, with intensities ranging from 5 to 1,250 with an average of 62 worms/case. Taenia saginata (7 cases) and Enterobius vermicularis (1 case) were other helminths recovered as the co-infectants. In Lampang Province, H. taichui was recovered from 69 cases (69.0%). The number of flukes recovered ranged from 1 to 4,277, with an average of 326 worms/case. Four cases had Phaneropsolus bonnei, and 10 T. saginata as the co-infectants. Adult specimens of O. viverrini were not recovered from any stool. Clearly, MIF infection, especially haplorchiasis, is more common in northern Thailand. These findings should encourage the Public Health Office to employ more specific tools than Kato's method for surveillance of opisthorchiasis in Thailand.

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  • Prevalence and Epidemiological Patterns of Enterobius vermicularis Infection in Thailand: A Systematic Review and Meta-Analysis
    Jurairat Jongthawin, Aongart Mahittikorn, Apiporn Thinkhamrop Suwannatrai, Chutima Rattanawan, Kinley Wangdi, Frederick Ramirez Masangkay, Manas Kotepui
    Medical Sciences.2025; 13(4): 207.     CrossRef
  • Study of the population genetic structure of Opisthorchis-like eggs in northern Thailand using mitochondrial genes
    Picha Suwannahitatorn, Mathirut Mungthin, Ittisak Subrungruang, Lakhanawan Charoensuk, Nithikoon Aksorn, Saiwasan Buathong, Krystyna Cwiklinski
    PLOS Neglected Tropical Diseases.2024; 18(8): e0012445.     CrossRef
  • Gastrointestinal Helminthic Infection among the Population in Northern Thailand
    Ampas Wisetmora, Oranard Wattanawong, Adulsak Wijit, Jutikarn Phukowluan, Ampol Nachairan, Prueksarawuth Jaksuay, Sivapong Sungpradit, Nuttapon Ekobol, Thidarut Boonmars, Alisa Boonsuya, Phornphitcha Pechdee, Chutharat Thanchonnang, Nav La, Nathkapach K.
    Acta Parasitologica.2024; 69(3): 1648.     CrossRef
  • Model-based spatial-temporal mapping of opisthorchiasis in endemic countries of Southeast Asia
    Ting-Ting Zhao, Yi-Jing Feng, Pham Ngoc Doanh, Somphou Sayasone, Virak Khieu, Choosak Nithikathkul, Men-Bao Qian, Yuan-Tao Hao, Ying-Si Lai
    eLife.2021;[Epub]     CrossRef
  • Adenosine Suppresses Cholangiocarcinoma Cell Growth and Invasion in Equilibrative Nucleoside Transporters-Dependent Pathway
    Kornkamon Lertsuwan, Supathra Phoaubon, Nathapol Tasnawijitwong, Jomnarong Lertsuwan
    International Journal of Molecular Sciences.2020; 21(3): 814.     CrossRef
  • Intestinal parasites in rural communities in Nan Province, Thailand: changes in bacterial gut microbiota associated with minute intestinal fluke infection
    Ajala Prommi, Pinidphon Prombutara, Dorn Watthanakulpanich, Poom Adisakwattana, Teera Kusolsuk, Tippayarat Yoonuan, Akkarin Poodeepiyasawat, Nirundorn Homsuwan, Samreong Prummongkol, Malee Tanita, Sungkhom Rattanapikul, Chuanphot Thinphovong, Anamika Krit
    Parasitology.2020; 147(9): 972.     CrossRef
  • Genetic Differentiation of Opisthorchis-Like Eggs in Northern Thailand Using Stool Specimens Under National Strategic Plan to Control Liver Fluke Infection and Cholangiocarcinoma
    Saiwasan Buathong, Kateitsaray Phaiphilai, Toon Ruang-areerate, Naruemon Sitthichot, Thanyapit Thita, Mathirut Mungthin, Picha Suwannahitatorn
    The American Journal of Tropical Medicine and Hygiene.2020; 103(3): 1118.     CrossRef
  • Parasitic infections in relation to practices and knowledge in a rural village in Northern Thailand with emphasis on fish-borne trematode infection
    K. Chaisiri, C. Jollivet, P. Della Rossa, S. Sanguankiat, D. Wattanakulpanich, C. Lajaunie, A. Binot, M. Tanita, S. Rattanapikul, D. Sutdan, S. Morand, A. Ribas
    Epidemiology and Infection.2019;[Epub]     CrossRef
  • Fishborne zoonotic heterophyid infections: An update
    Jong-Yil Chai, Bong-Kwang Jung
    Food and Waterborne Parasitology.2017; 8-9: 33.     CrossRef
  • Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand
    Saiwasan Buathong, Saovanee Leelayoova, Mathirut Mungthin, Toon Ruang-areerate, Tawee Naaglor, Picha Suwannahitatorn, Phunlerd Piyaraj, Paanjit Taamasri, Peerapan Tan-ariya, Edoardo Pozio
    PLOS Neglected Tropical Diseases.2017; 11(11): e0006030.     CrossRef
  • 7,465 View
  • 94 Download
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Original Articles

A Cross-Sectional Study on Intestinal Parasitic Infections in Rural Communities, Northeast Thailand
Sirintip Boonjaraspinyo, Thidarut Boonmars, Butsara Kaewsamut, Nuttapon Ekobol, Porntip Laummaunwai, Ratchadawan Aukkanimart, Nadchanan Wonkchalee, Amornrat Juasook, Pranee Sriraj
Korean J Parasitol 2013;51(6):727-734.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.727

Despite the existence of effective anthelmintics, parasitic infections remain a major public health problem in Southeast Asia, including Thailand. In rural communities, continuing infection is often reinforced by dietary habits that have a strong cultural basis and by poor personal hygiene and sanitation. This study presents a survey of the prevalence of intestinal parasitic infections among the people in rural Thailand. The community-based cross-sectional study was conducted in villages in Khon Kaen Province, northeastern Thailand, from March to August 2013. A total of 253 stool samples from 102 males and 140 females, aged 2-80 years, were prepared using formalin-ethyl acetate concentration methods and examined using light microscopy. Ninety-four individuals (37.2%) were infected with 1 or more parasite species. Presence of parasitic infection was significantly correlated with gender (P=0.001); nearly half of males in this survey (49.0%) were infected. Older people had a higher prevalence than younger members of the population. The most common parasite found was Opisthorchis viverrini (26.9%), followed by Strongyloides stercoralis (9.5%), Taenia spp. (1.6%), echinostomes (0.4%), and hookworms (0.4%). The prevalence of intestinal protozoa was Blastocystis hominis 1.6%, Entamoeba histolytica 0.8%, Entamoeba coli 0.8%, Balantidium coli 0.4%, Iodamoeba b?tschlii 0.4%, and Sarcocystis hominis 0.4%. Co-infections of various helminths and protozoa were present in 15.9% of the people. The present results show that the prevalence of parasitic infections in this region is still high. Proactive education about dietary habits, personal hygiene, and sanitation should be provided to the people in this community to reduce the prevalence of intestinal parasite infections. Moreover, development of policies and programs to control parasites is needed.

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Zoonotic Echinostome Infections in Free-Grazing Ducks in Thailand
Weerachai Saijuntha, Kunyarat Duenngai, Chairat Tantrawatpan
Korean J Parasitol 2013;51(6):663-667.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.663

Free-grazing ducks play a major role in the rural economy of Eastern Asia in the form of egg and meat production. In Thailand, the geographical location, tropical climate conditions and wetland areas of the country are suitable for their husbandry. These environmental factors also favor growth, multiplication, development, survival, and spread of duck parasites. In this study, a total of 90 free-grazing ducks from northern, central, and northeastern regions of Thailand were examined for intestinal helminth parasites, with special emphasis on zoonotic echinostomes. Of these, 51 (56.7%) were infected by one or more species of zoonotic echinostomes, Echinostoma revolutum, Echinoparyphium recurvatum, and Hypoderaeum conoideum. Echinostomes found were identified using morphological criteria when possible. ITS2 sequences were used to identify juvenile and incomplete worms. The prevalence of infection was relatively high in each region, namely, north, central, and northeast region was 63.2%, 54.5%, and 55.3%, respectively. The intensity of infection ranged up to 49 worms/infected duck. Free-grazing ducks clearly play an important role in the life cycle maintenance, spread, and transmission of these medically important echinostomes in Thailand.

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Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp
Ratchadawan Aukkanimart, Thidarut Boonmars, Somchai Pinlaor, Smarn Tesana, Surasit Aunpromma, Chantana Booyarat, Pranee Sriraj, Porntip Laummaunwai, Wiyada Punjaruk
Korean J Parasitol 2013;51(5):537-544.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.537

The present study was performed to observe histopathological changes in tissues of Bithynia siamensis goniomphalos (Gastropoda, Bithyniidae) incubated in crude extract solutions of camellia (Camellia oleifera) seed and mangosteen (Garcinia mangostana) pericarp, and furthermore to estimate the molluscicidal effects of 2 plant substances. Substantial numbers of bithyniid snails were incubated in various concentrations of 2 plant solution for 24 hr. As the positive control, snails incubated in various concentrations of niclosamide, a chemical molluscicide, were used. The histopathological findings were observed in sectioned snail specimens of each experimental and control groups. The results showed that both camellia and mangosteen extracts had molluscicidal effects at 24 hr with 50% lethal concentration (LC50) at concentrations of 0.003 and 0.002 g/ml, respectively, while niclosamide had LC50 at concentrations 0.599 ppm. B. siamensis goniomphalos snail tissues (foot, gill, and digestive system) showed disruption of columnar muscle fibers of the foot, reduction of the length and number of gill cilia, numerous mucous vacuoles, and irregularly shaped of epithelial cells. Irregular apical and calciferous cells, dilatation of the digestive gland tubule, and large hemolymphatic spaces, and irregular apical surfaces, detachment of cilia, and enlargement of lysosomal vacuoles of epidermis were also shown in all groups. By the present study, it is confirmed that 2 plants, camellia and mangosteen, are keeping some substance having molluscicidal effects, and histopathological findings obtained in this study will provide some clues in further studies on their action mechanisms to use them as natural molluscicides.

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Echinostoma revolutum: Freshwater Snails as the Second Intermediate Hosts in Chiang Mai, Thailand
Kittichai Chantima, Jong-Yil Chai, Chalobol Wongsawad
Korean J Parasitol 2013;51(2):183-189.
Published online April 25, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.2.183

The occurrence of 37-collar spined echinostome metacercariae in freshwater snails was investigated in 6 districts of Chiang Mai Province, Thailand, from October 2011 to April 2012. A total of 2,914 snails that belong to 12 species were examined, and 7 snail species (Clea helena, Eyriesia eyriesi, Bithynia funiculata, Bithynia siamensis siamensis, Filopaludina doliaris, Filopaludina sumatrensis polygramma, and Filopaludina martensi martensi) were found infected with echinostome metacercariae. The prevalence of metacercariae was the highest in Filopaludina spp. (38.5-58.7%) followed by B. funiculata (44.0%), E. eyriesi (12.5%), B. siamensis siamensis (8.2%), and C. helena (5.1%). Metacercariae were experimentally fed to hamsters and domestic chicks, and adult flukes were recovered from both hosts at days 15 and 20 post-infection. The adult flukes were identified based on morphological features, morphometrics, host-parasite relationships, and geographical distribution. They were compatible to Echinostoma revolutum or Echinostoma jurini, with only minor differences. As the adults were recovered from both hamsters and chicks, our specimens were more compatible to E. revolutum rather than E. jurini (reported only from mammals). This is the first report for metacercariae of E. revolutum in the snail host, C. helena, and also confirmed that Filopaludina spp., E. eryresi, and Bithynia spp. act as the second intermediate hosts of E. revolutum under natural conditions, which are indigenously distributed in Chiang Mai province.

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    The Korean Journal of Parasitology.2020; 58(4): 343.     CrossRef
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    K. Chantima, C. Rika
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    Preeyaporn Butboonchoo, Chalobol Wongsawad, Pheravut Wongsawad, Jong-Yil Chai
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Articles from Symposium on Asian Taenia (October 2011, Osong, Korea)

Genetic Diversity of Taenia asiatica from Thailand and Other Geographical Locations as Revealed by Cytochrome c Oxidase Subunit 1 Sequences
Malinee Thairungroj Anantaphruti, Urusa Thaenkham, Dorn Watthanakulpanich, Orawan Phuphisut, Wanna Maipanich, Tippayarat Yoonuan, Supaporn Nuamtanong, Somjit Pubampen, Surapol Sanguankiat
Korean J Parasitol 2013;51(1):55-59.
Published online February 18, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.1.55

Twelve 924 bp cytochrome c oxidase subunit 1 (cox1) mitochondrial DNA sequences from Taenia asiatica isolates from Thailand were aligned and compared with multiple sequence isolates from Thailand and 6 other countries from the GenBank database. The genetic divergence of T. asiatica was also compared with Taenia saginata database sequences from 6 different countries in Asia, including Thailand, and 3 countries from other continents. The results showed that there were minor genetic variations within T. asiatica species, while high intraspecies variation was found in T. saginata. There were only 2 haplotypes and 1 polymorphic site found in T. asiatica, but 8 haplotypes and 9 polymorphic sites in T. saginata. Haplotype diversity was very low, 0.067, in T. asiatica and high, 0.700, in T. saginata. The very low genetic diversity suggested that T. asiatica may be at a risk due to the loss of potential adaptive alleles, resulting in reduced viability and decreased responses to environmental changes, which may endanger the species.

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  • Taenia asiatica: Mitochondrial signatures based analysis of an emerging public health threat in India
    Aman D. Moudgil, Anil K. Nehra, Pallavi Moudgil
    New Microbes and New Infections.2025; 63: 101562.     CrossRef
  • Identification of species and genetic variation in Taenia isolates from human and swine of North India
    Satyendra K. Singh, Kashi N. Prasad, Aloukick K. Singh, Kamlesh K. Gupta, Ranjeet S. Chauhan, Amrita Singh, Avinash Singh, Ravi P. Rai, Binod K. Pati
    Parasitology Research.2016; 115(10): 3689.     CrossRef
  • Epidemiology and genetic diversity of Taenia asiatica: a systematic review
    Anita Ale, Bjorn Victor, Nicolas Praet, Sarah Gabriël, Niko Speybroeck, Pierre Dorny, Brecht Devleesschauwer
    Parasites & Vectors.2014;[Epub]     CrossRef
  • Current Status of Taeniasis in Thailand
    Malinee Thairungroj Anantaphruti
    The Korean Journal of Parasitology.2013; 51(1): 37.     CrossRef
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Original Articles

Positivity and Intensity of Gnathostoma spinigerum Infective Larvae in Farmed and Wild-Caught Swamp Eels in Thailand
Wilai Saksirisampant, Benjamas Wongsatayanon Thanomsub
Korean J Parasitol 2012;50(2):113-118.
Published online May 24, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.2.113

From July 2008 to June 2009, livers of the swamp eels (Monopterus alba) were investigated for advanced third-stage larvae (AL3) of Gnathostoma spinigerum. Results revealed that 10.2% (106/1,037) and 20.4% (78/383) of farmed eels from Aranyaprathet District, Sa Kaeo Province and those of wild-caught eels obtained from a market in Min Buri District of Bangkok, Thailand were infected, respectively. The prevalence was high during the rainy and winter seasons. The infection rate abruptly decreased in the beginning of summer. The highest infection rate (13.7%) was observed in September and absence of infection (0%) in March-April in the farmed eels. Whereas, in the wild-caught eels, the highest rate (30.7%) was observed in November, and the rate decreased to the lowest at 6.3% in March. The average no. (mean±SE) of AL3 per investigated liver in farmed eels (1.1±0.2) was significantly lower (P=0.040) than those in the caught eels (0.2±0.03). In addition, the intensity of AL3 recovered from each infected liver varied from 1 to 18 (2.3±0.3) in the farmed eels and from 1 to 47 (6.3±1.2) in the caught eels, respectively. The AL3 intensity showed significant difference (P=0.011) between these 2 different sources of eels. This is the first observation that farmed eels showed positive findings of G. spinigerum infective larvae. This may affect the standard farming of the culture farm and also present a risk of consuming undercooked eels from the wild-caught and farmed eels.

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    Kathyleen Nogrado, Poom Adisakwattana, Onrapak Reamtong
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    Sixin Zhang, Guangping Huang, Liang Li, Xianyong Liu, Xiaoli Tang, Xun Suo
    Pathogens.2021; 10(6): 711.     CrossRef
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    Acta Tropica.2020; 204: 105288.     CrossRef
  • Infection Status of Gnathostoma spinigerum Larvae in Asian Swamp Eels, Monopterus albus, Purchased from Local Markets in Cambodia
    Jong-Yil Chai, Bong-Kwang Jung, Keon Hoon Lee, Sung-Jong Hong, Virak Khieu, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2020; 58(6): 695.     CrossRef
  • Genetic diversity of infective larvae of Gnathostoma spinigerum (Nematoda: Gnathostomatidae) in freshwater swamp eels from Thailand
    P. Eamsobhana, D. Wanachiwanawin, K. Roongruangchai, S.L. Song, H.S. Yong
    Journal of Helminthology.2017; 91(6): 767.     CrossRef
  • Cloning and expression analysis of two pro-inflammatory cytokines, IL-1β and its receptor, IL-1R2, in the Asian swamp eel Monopterus albus
    Q. Q. Xu, P. Xu, J. W. Zhou, T. S. Pan, R. Tuo, K. Ai, D. Q. Yang
    Molecular Biology.2016; 50(5): 671.     CrossRef
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    Qiaoqing Xu, Jin Wan, Tingshuang Pan, Jingwen Zhou, Kete Ai, Daiqin Yang
    Genes & Genomics.2015; 37(5): 429.     CrossRef
  • Larval Gnathostoma spinigerum Detected in Asian Swamp Eels, Monopterus albus, Purchased from a Local Market in Yangon, Myanmar
    Jong-Yil Chai, Woon-Mok Sohn, Byoung-Kuk Na, Jong-Bok Park, Hoo-Gn Jeoung, Eui-Hyug Hoang, Thi Thi Htoon, Htay Htay Tin
    The Korean Journal of Parasitology.2015; 53(5): 619.     CrossRef
  • Cutaneous Gnathostomiasis with Recurrent Migratory Nodule and Persistent Eosinophilia: a Case Report from China
    Jing Cui, Ye Wang, Zhong Quan Wang
    The Korean Journal of Parasitology.2013; 51(4): 467.     CrossRef
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Evaluation of Rapid Diagnostics for Plasmodium falciparum and P. vivax in Mae Sot Malaria Endemic Area, Thailand
Wanna Chaijaroenkul, Thanee Wongchai, Ronnatrai Ruangweerayut, Kesara Na-Bangchang
Korean J Parasitol 2011;49(1):33-38.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.33

Prompt and accurate diagnosis of malaria is the key to prevent disease morbidity and mortality. This study was carried out to evaluate diagnostic performance of 3 commercial rapid detection tests (RDTs), i.e., Malaria Antigen Pf/Pan™, Malaria Ag-Pf™, and Malaria Ag-Pv™ tests, in comparison with the microscopic and PCR methods. A total of 460 blood samples microscopically positive for Plasmodium falciparum (211 samples), P. vivax (218), mixed with P. falciparum and P. vivax (30), or P. ovale (1), and 124 samples of healthy subjects or patients with other fever-related infections, were collected. The sensitivities of Malaria Ag-Pf™ and Malaria Antigen Pf/Pan™ compared with the microscopic method for P. falciparum or P. vivax detection were 97.6% and 99.0%, or 98.6% and 99.0%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, and Malaria Antigen Pf/Pan™ were 93.3%, 98.8%, and 94.4%, respectively. The sensitivities of Malaria Ag-Pf™, Malaria Antigen Pf/Pan™, and microscopic method, when PCR was used as a reference method for P. falciparum or P. vivax detection were 91.8%, 100%, and 96.7%, or 91.9%, 92.6%, and 97.3%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, Malaria Antigen Pf/Pan™, and microscopic method were 66.2%, 92.7%, 73.9%, and 78.2%, respectively. Results indicated that the diagnostic performances of all the commercial RDTs are satisfactory for application to malaria diagnosis.

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    Vipin Kumar, Sandipan Ray, Shalini Aggarwal, Deeptarup Biswas, Manali Jadhav, Radha Yadav, Sanjeev V. Sabnis, Soumaditya Banerjee, Arunansu Talukdar, Sanjay K. Kochar, Suvin Shetty, Kunal Sehgal, Swati Patankar, Sanjeeva Srivastava
    Communications Biology.2020;[Epub]     CrossRef
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    Endale TADESSE, Bereket WORKALEMAHU, Techalew SHIMELIS
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    Shyamapada Mandal
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    Pimwan Thongdee, Wanna Chaijaroenkul, Jiraporn Kuesap, Kesara Na-Bangchang
    The Korean Journal of Parasitology.2014; 52(4): 377.     CrossRef
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    Katharine Abba, Amanda J Kirkham, Piero L Olliaro, Jonathan J Deeks, Sarah Donegan, Paul Garner, Yemisi Takwoingi
    Cochrane Database of Systematic Reviews.2014;[Epub]     CrossRef
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Brief Communication

Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach
Thapana Chontananarth, Chalobol Wongsawad
Korean J Parasitol 2010;48(4):343-346.
Published online December 16, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.4.343

The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.

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    Zoosystematics and Evolution.2020; 96(2): 807.     CrossRef
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    Thapana Chontananarth, Sothorn Anucherngchai, Thanawan Tejangkura
    Journal of Parasitic Diseases.2018; 42(3): 423.     CrossRef
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    Duangduen Krailas, Suluck Namchote, Tunyarut Koonchornboon, Wivitchuta Dechruksa, Dusit Boonmekam
    Zoosystematics and Evolution.2014; 90(1): 57.     CrossRef
  • Molecular phylogeny of trematodes in Family Heterophyidae based on mitochondrial cytochrome c oxidase subunit I (mCOI)
    Thapana Chontananarth, Chalobol Wongsawad, Siriwadee Chomdej, Duangduen Krailas, Jong Yil Chai
    Asian Pacific Journal of Tropical Medicine.2014; 7(6): 446.     CrossRef
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    Thapana Chontananarth, Chalobol Wongsawad
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Case Report

Anchitrema sanguineum (Digenea: Anchitrematidae) Accidentally Found during Colonoscopy of a Patient with Chronic Abdominal Pain: A Case Report
Teera Kusolsuk, Nantana Paiboon, Somchit Pubampen, Wanna Maipanich, Paron Dekumyoy, Jitra Waikagul
Korean J Parasitol 2009;47(2):167-170.
Published online May 27, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.2.167

In November 2007, a 46-year-old male Thai patient presented with chronic abdominal pain for over 3 years. Colonoscopy revealed a small parasite of about 2 × 1 mm in size attached to the cecum mucosa. The worm was removed endoscopically, fixed, and stained for morphological observations. The specimen was identified as Anchitrema sanguineum (Digenea: Anchitrematidae), a trematode first reported in a reptile, Chamaeleo vulgaris, from Egypt, and then sporadically found in the intestines of insectivorous bats and other mammals. The patient was treated with praziquantel but no more worms were found in his stool. His symptoms improved slightly but not cured completely. It remains unclear whether the chronic abdominal pain of the patient was caused by this trematode infection. Whatever is the pathogenicity of this trematode, this is the first human case of A. sanguineum infection in the literature.

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    Daniel C G Metz, Andrew V Turner, Alexandria P Nelson, Ryan F Hechinger
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  • Mini-FLOTAC as an alternative, non-invasive diagnostic tool for Schistosoma mansoni and other trematode infections in wildlife reservoirs
    Stefano Catalano, Amelia Symeou, Kirsty J. Marsh, Anna Borlase, Elsa Léger, Cheikh B. Fall, Mariama Sène, Nicolas D. Diouf, Davide Ianniello, Giuseppe Cringoli, Laura Rinaldi, Khalilou Bâ, Joanne P. Webster
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Original Articles

Drug Resistance and in Vitro Susceptibility of Plasmodium falciparum in Thailand during 1988-2003
Nantana Suwandittakul, Wanna Chaijaroenkul, Pongchai Harnyuttanakorn, Mathirut Mungthin, Kesara Na Bangchang
Korean J Parasitol 2009;47(2):139-144.
Published online May 27, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.2.139

The aim of the present study was to investigate antimalarial drug pressure resulting from the clinical use of different antimalarials in Thailand. The phenotypic diversity of the susceptibility profiles of antimalarials, i.e., chloroquine (CQ), quinine (QN), mefloquine (MQ), and artesunate (ARS) in Plasmodium falciparum isolates collected during the period from 1988 to 2003 were studied. P. falciparum isolates from infected patients were collected from the Thai-Cambodian border area at different time periods (1988-1989, 1991-1992, and 2003), during which 3 different patterns of drug use had been implemented: MQ + sulphadoxine (S) + pyrimethamine (P), MQ alone and MQ + ARS, respectively. The in vitro drug susceptibilities were investigated using a method based on the incorporation of [3H] hypoxanthine. A total of 50 isolates were tested for susceptibilities to CQ, QN, MQ, and ARS. Of these isolates, 19, 16, and 15 were adapted during the periods 1988-1989, 1991-1993, and 2003, respectively. P. falciparum isolates collected during the 3 periods were resistant to CQ. Sensitivities to MQ declined from 1988 to 2003. In contrast, the parasite was sensitive to QN, and similar sensitivity profile patterns were observed during the 3 time periods. There was a significantly positive but weak correlation between the IC50 values of CQ and QN, as well as between the IC50 values of QN and MQ. Drug pressure has impact on sensitivity of P. falciparum to MQ. A combination therapy of MQ and ARS is being applied to reduce the parasite resistance, and also increasing the efficacy of the drug.

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Gametocyte Clearance in Uncomplicated and Severe Plasmodium falciparum Malaria after Artesunate-Mefloquine Treatment in Thailand
Noppadon Tangpukdee, Srivicha Krudsood, Sriripun Srivilairit, Nanthaporn Phophak, Putza Chonsawat, Wimon Yanpanich, Shigeyuki Kano, Polrat Wilairatana
Korean J Parasitol 2008;46(2):65-70.
Published online June 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.2.65

Artemisinin-based combination therapy (ACT) is currently promoted as a strategy for treating both uncomplicated and severe falciparum malaria, targeting asexual blood-stage Plasmodium falciparum parasites. However, the effect of ACT on sexual-stage parasites remains controversial. To determine the clearance of sexual-stage P. falciparum parasites from 342 uncomplicated, and 217 severe, adult malaria cases, we reviewed and followed peripheral blood sexual-stage parasites for 4 wk after starting ACT. All patients presented with both asexual and sexual stage parasites on admission, and were treated with artesunate-mefloquine as the standard regimen. The results showed that all patients were asymptomatic and negative for asexual forms before discharge from hospital. The percentages of uncomplicated malaria patients positive for gametocytes on days 3, 7, 14, 21, and 28 were 41.5, 13.1, 3.8, 2.0, and 2.0%, while the percentages of gametocyte positive severe malaria patients on days 3, 7, 14, 21, and 28 were 33.6, 8.2, 2.7, 0.9, and 0.9%, respectively. Although all patients were negative for asexual parasites by day 7 after completion of the artesunate-mefloquine course, gametocytemia persisted in some patients. Thus, a gametocytocidal drug, e.g., primaquine, may be useful in combination with an artesunate-mefloquine regimen to clear gametocytes, so blocking transmission more effectively than artesunate alone, in malaria transmission areas.

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Brief Communication

Prevalence of Haplorchis taichui and Haplorchoides sp. Metacercariae in Freshwater Fish from Water Reservoirs, Chiang Mai, Thailand
Choosak Nithikathkul, Chalobol Wongsawad
Korean J Parasitol 2008;46(2):109-112.
Published online June 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.2.109

A parasitological investigation on trematode metacercariae was made on 62 freshwater fishes of 13 species in northern Thailand; Cyclocheilichthys apogon, Puntioplites proctozysron, Labiobarbus siamensis, Barbodes gonionotus, Barbodes altus, Henicorhynchus siamensis, Osteochilus hasselti, Notopterus notopterus, Mystacoleucus marginatus, Anabas testudineus, Systomus orphoides, Morulius chrysophykadian, and Hampala macrolepidota. The fish were caught over the summer period (February-May 2007) from 2 Chiang Mai water reservoirs, i.e., the Mae Ngad (UTM 47Q E 503200, 47Q N 2119300) and the Mae Kuang Udomtara (UTM 47Q E 513000, 47Q N 2092600) Reservoirs in Chiang Mai province, Thailand. The prevalence of heterophyid (Haplorchis taichui and Haplorchoides sp.) metacercariae in these fish was 83.9% and 74.2% in the Mae Ngad and Mae Kuang Udomtara Reservoirs, respectively. The highest intensity of heterophyid metacercariae in H. siamensis in the Mae Ngad was 120.4 and that in P. proctozysron in the Mae Kuang Udomtara was 180.0. The fish, A. testudineus, C. apogon, and M. chrysophykadian, were not found to be infected with H. taichui metacercariae. The results show that the freshwater fish in Chiang Mai water reservoirs are heavily infected with H. taichui and Haplorchoides sp. metacercariae.

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    K. Apiwong, Ch. Wongsawad, P. Butboonchoo
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Original Articles

Clinical efficacy of chloroquine versus artemether-lumefantrine for Plasmodium vivax treatment in Thailand
Srivicha Krudsood, Noppadon Tangpukdee, Sant Muangnoicharoen, Vipa Thanachartwet, Nutthanej Luplertlop, Siripan Srivilairit, Polrat Wilairatana, Shigeyuki Kano, Pascal Ringwald, Sornchai Looareesuwan
Korean J Parasitol 2007;45(2):111-114.
Published online June 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.2.111

Chloroquine remains the drug of choice for the treatment of vivax malaria in Thailand. Mixed infections of falciparum and vivax malaria are also common in South-East Asia. Laboratory confirmation of malaria species is not generally available. This study aimed to find alternative regimens for treating both malaria species by using falciparum antimalarial drugs. From June 2004 to May 2005, 98 patients with Plasmodium vivax were randomly treated with either artemether-lumefantrine (n = 47) or chloroquine (n = 51). Both treatments were followed by 15 mg of primaquine over 14 days. Adverse events and clinical and parasitological outcomes were recorded and revealed similar in both groups. The cure rate was 97.4% for the artemether-lumefantrine treated group and 100% for the chloroquine treated group. We concluded that the combination of artemether-lumefantrine and primaquine was well tolerated, as effective as chloroquine and primaquine, and can be an alternative regimen for treatment of vivax malaria especially in the event that a mixed infection of falciparum and vivax malaria could not be ruled out.

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    Thanawat Sridapan, Paweesuda Rattanakoch, Kaewkanha Kijprasong, Suttipat Srisutham, Kristan Alexander Schneider
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Travelers' malaria among foreigners at the Hospital for Tropical Diseases, Bangkok, Thailand: a 6-year review (2000-2005)
Watcharapong Piyaphanee, Srivicha Krudsood, Udomsak Silachamroon, Karnchana Pornpininworakij, Phatcharee Danwiwatdecha, Supat Chamnachanan, Polrat Wilairatana, Sornchai Looareesuwan
Korean J Parasitol 2006;44(3):229-232.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.229

We retrospectively examined the charts of travelers admitted to the Hospital for Tropical Diseases, Bangkok, Thailand, with malaria during the years 2000-2005. Twenty-one cases of malaria were identified, of which 12 (57%) were Plasmodium vivax infections and 9 (43%) were P. falciparum infections. There was one mixed case with vivax and falciparum infection. Only 1 P. falciparum case had complications. All cases were successfully treated with standard antimalarial drugs. Only 3 of the 21 cases were thought to be acquired in Thailand, the rest were regarded to be imported.

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Safety and tolerability of elubaquine (bulaquine, CDRI 80/53) for treatment of Plasmodium vivax malaria in Thailand
Srivicha Krudsood, Polrat Wilairatana, Noppadon Tangpukdee, Kobsiri Chalermrut, Siripun Srivilairit, Vipa Thanachartwet, Sant Muangnoicharoen, Natthanej Luplertlop, Gary M. Brittenham, Sornchai Looareesuwan
Korean J Parasitol 2006;44(3):221-228.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.221

We conducted a study to compare the safety and tolerability of anti-relapse drugs elubaquine and primaquine against Plasmodium vivax malaria. After standard therapy with chloroquine, 30 mg/kg given over 3 days, 141 patients with P. vivax infection were randomized to receive primaquine or elubaquine. The 2 treatment regimens were primaquine 30 mg once daily for 7 days (group A, n = 71), and elubaquine 25 mg once daily for 7 days (group B, n = 70). All patients cleared parasitemia within 7 days after chloroquine treatment. Among patients treated with primaquine, one patient relapsed on day 26; no relapse occurred with elubaquine treatement. Both drugs were well tolerated. Adverse effects occurred only in patients with G6PD deficiency who were treated with primaquine (group A, n = 4), whose mean hematocrit fell significantly on days 7, 8 and 9 (P = 0.015, 0.027, and 0.048, respectively). No significant change in hematocrit was observed in patients with G6PD deficiency who were treated with elubaquine (group B, n = 3) or in patients with normal G6PD. In conclusion, elubaquine, as anti-relapse therapy for P. vivax malaria, was as safe and well tolerated as primaquine and did not cause clinically significant hemolysis.

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Case Report
Peripheral gangrene in patients with severe falciparum malaria: report of 3 cases
Vipa Thanachartwet, Srivicha Krudsood, Polrat Wilairatana, Weerapong Phumratanaprapin, Udomsak Silachamroon, Sornchai Looareesuwan
Korean J Parasitol 2006;44(2):139-143.
Published online June 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.2.139

Peripheral gangrene, characterized by distal ischemia of the extremities, is a rare complication in patients with falciparum malaria. Patients with this complication have generally undergone early amputation of the affected areas. In this report, we describe 3 adult Thai patients presented at the Hospital for Tropical Diseases, Bangkok, with high grade of fever ranged 6-9 days, jaundice, acute renal failure, respiratory failure, alteration of consciousness and shock. Two patients had gangrene developed at the lower extremities on day 1 of hospitalization and 1 patient had gangrene developed on day 3. Blood smears revealed hyperparasitemia with Plasmodium falciparum. These patients were diagnosed as having severe malaria with peripheral gangrene. The resolution of gangrene was successfully achieved by treatment with artesunate and conservative treatment in 2 of 3 cases.

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