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"Soon-Hyung Lee"

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"Soon-Hyung Lee"

Original Articles

Prevalence of Intestinal Helminths among Inhabitants of Cambodia (2006-2011)
Tai-Soon Yong, Jong-Yil Chai, Woon-Mok Sohn, Keeseon S. Eom, Hoo-Gn Jeoung, Eui-Hyug Hoang, Cheong-Ha Yoon, Bong-Kwang Jung, Soon-Hyung Lee, Muth Sinuon, Duong Socheat
Korean J Parasitol 2014;52(6):661-666.
Published online December 23, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.6.661

In order to investigate the status of intestinal helminthic infections in Cambodia, epidemiological surveys were carried out on a national scale, including 19 provinces. A total of 32,201 fecal samples were collected from schoolchildren and adults between 2006 and 2011 and examined once by the Kato-Katz thick smear technique. The overall egg positive rate of intestinal helminths was 26.2%. The prevalence of hookworms was the highest (9.6%), followed by that of Opisthorchis viverrini/minute intestinal flukes (Ov/MIF) (5.7%), Ascaris lumbricoides (4.6%), and Trichuris trichiura (4.1%). Other types of parasites detected were Enterobius vermicularis (1.1%), Taenia spp. (0.4%), and Hymenolepis spp. (0.2%). The northwestern regions such as the Siem Reap, Oddar Meanchey, and Banteay Meanchey Provinces showed higher prevalences (17.4-22.3%) of hookworms than the other localities. The southwestern areas, including Koh Kong and Preah Sihanouk Provinces showed higher prevalences of A. lumbricoides (17.5-19.2%) and T. trichiura (6.1-21.0%). Meanwhile, the central and southern areas, in particular, Takeo and Kampong Cham Provinces, showed high prevalences of Ov/MIF (23.8-24.0%). The results indicate that a considerably high prevalence of intestinal helminths has been revealed in Cambodia, and thus sustained national parasite control projects are necessary to reduce morbidity due to parasitic infections in Cambodia.

Citations

Citations to this article as recorded by  Crossref logo
  • Prevalence and Epidemiological Patterns of Enterobius vermicularis Infection in Thailand: A Systematic Review and Meta-Analysis
    Jurairat Jongthawin, Aongart Mahittikorn, Apiporn Thinkhamrop Suwannatrai, Chutima Rattanawan, Kinley Wangdi, Frederick Ramirez Masangkay, Manas Kotepui
    Medical Sciences.2025; 13(4): 207.     CrossRef
  • Prevalence of Foodborne Trematodes in Small Indigenous Fish Species in Local Markets of Phnom Penh, Cambodia
    Domenico Caruso, Lin Kong, Samnang Keo, Sreyni Yoeurn, Samphal Seng
    Vector-Borne and Zoonotic Diseases.2024; 24(3): 141.     CrossRef
  • Prevalence of Intestinal Helminths Among Mogadishu Residents Attending Somali Sudanese Specialized Hospital, Mogadishu, Somalia
    Motaz Mohammed
    American Journal of Laboratory Medicine.2024; 9(1): 8.     CrossRef
  • Evaluation of the diagnostic techniques in the detection of hookworm infestation among school children in Ethiopia: Cross-sectional study design
    Abebe Fenta, Destaw Kebede, Adane Tilahun, Bewket Mesganaw, Adane Adugna, Wubetu Yihunie, Habtamu Belew, Desalegn Abebaw, Gashaw Azanaw Amare
    Heliyon.2024; 10(21): e39936.     CrossRef
  • A study protocol for a multi-country cluster randomized controlled trial of the impact of a multi-component One Health strategy to eliminate Opisthorchis viverrini and soil transmitted helminths in the Lower Mekong Basin
    Mary Lorraine Mationg, Archie C. A. Clements, Gail M. Williams, Matthew Kelly, Donald E. Stewart, Catherine A. Gordon, Kinley Wangdi, Sirikachorn Tangkawattana, Apiporn T. Suwannatrai, Vanathom Savathdy, Visal Khieu, Sangduan Wannachart, Suji Yoo O’Connor
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  • An Integrated Coproscopic and Molecular Method Provides Insights into the Epidemiology of Zoonotic Intestinal Helminths of Dogs across Cambodia
    Patsy A. Zendejas-Heredia, Vito Colella, Lucas G. Huggins, Roland Schaper, Bettina Schunack, Rebecca J. Traub, Long-Xian Zhang
    Transboundary and Emerging Diseases.2023; 2023: 1.     CrossRef
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    PLOS Neglected Tropical Diseases.2023; 17(3): e0011073.     CrossRef
  • High endemicity of Opisthorchis viverrini infection among people in northern Cambodia confirmed by adult worm expulsion
    Bong-Kwang Jung, Sooji Hong, Taehee Chang, Jaeeun Cho, Seungwan Ryoo, Keon Hoon Lee, Jeonggyu Lee, Woon-Mok Sohn, Sung-Jong Hong, Virak Khieu, Rekol Huy, Jong-Yil Chai
    Scientific Reports.2023;[Epub]     CrossRef
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    Jong-Yil Chai, Bong-Kwang Jung
    Parasitology.2022; 149(10): 1262.     CrossRef
  • Prevalence and Associated Risk Factors of Human Intestinal Helminths Parasitic Infections in Ethiopia: A Systematic Review and Meta-Analysis
    Minichil Liyih, Destaw Damtie, Dires Tegen, Ahmad Mansour
    The Scientific World Journal.2022; 2022: 1.     CrossRef
  • Model-based spatial-temporal mapping of opisthorchiasis in endemic countries of Southeast Asia
    Ting-Ting Zhao, Yi-Jing Feng, Pham Ngoc Doanh, Somphou Sayasone, Virak Khieu, Choosak Nithikathkul, Men-Bao Qian, Yuan-Tao Hao, Ying-Si Lai
    eLife.2021;[Epub]     CrossRef
  • Prevalence of meat-transmitted Taenia and Trichinella parasites in the Far East countries
    Yi Liu, Zijian Dong, Jianda Pang, Mingyuan Liu, Xuemin Jin
    Parasitology Research.2021; 120(12): 4145.     CrossRef
  • Current status of human liver fluke infections in the Greater Mekong Subregion
    Banchob Sripa, Apiporn T. Suwannatrai, Somphou Sayasone, Dung Trung Do, Virak Khieu, Yichao Yang
    Acta Tropica.2021; 224: 106133.     CrossRef
  • Prevalence and Intensity of Opisthorchis viverrini Metacercarial Infection in Fish from Phnom Penh, Takeo, and Kandal Provinces, Cambodia
    Woon-Mok Sohn, Seung-Ho Choi, Bong-Kwang Jung, Sooji Hong, Seungwan Ryoo, Taehee Chang, Keon Hoon Lee, Byoung-Kuk Na, Sung-Jong Hong, Virak Khieu, Jong-Yil Chai
    The Korean Journal of Parasitology.2021; 59(5): 531.     CrossRef
  • Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia
    Ramon M. Eichenberger, Lian F. Thomas, Sarah Gabriël, Branco Bobić, Brecht Devleesschauwer, Lucy J. Robertson, Anastasios Saratsis, Paul R. Torgerson, Uffe C. Braae, Veronique Dermauw, Pierre Dorny
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Morphological and Molecular Diagnosis of Necator americanus and Ancylostoma ceylanicum Recovered from Villagers in Northern Cambodia
    Taehee Chang, Bong-Kwang Jung, Woon-Mok Sohn, Sooji Hong, Hyejoo Shin, Seungwan Ryoo, Jeonggyu Lee, Keon Hoon Lee, Virak Khieu, Rekol Huy, Jong-Yil Chai
    The Korean Journal of Parasitology.2020; 58(6): 619.     CrossRef
  • Low-Grade Endemicity of Opisthorchiasis, Yangon, Myanmar
    Woon-Mok Sohn, Bong-Kwang Jung, Sung-Jong Hong, Keon-Hoon Lee, Jong-Bok Park, Hyun-Seung Kim, Seon Cho, Thi Thi Htoon, Htay Htay Tin, Jong-Yil Chai
    Emerging Infectious Diseases.2019; 25(7): 1435.     CrossRef
  • Thiamine dose response in human milk with supplementation among lactating women in Cambodia: study protocol for a double-blind, four-parallel arm randomised controlled trial
    Kyly C Whitfield, Hou Kroeun, Tim Green, Frank T Wieringa, Mam Borath, Prak Sophonneary, Jeffrey R Measelle, Dare Baldwin, Lisa N Yelland, Shalem Leemaqz, Kathleen Chan, Jelisa Gallant
    BMJ Open.2019; 9(7): e029255.     CrossRef
  • Molecular Identification of Trichuris suis and Trichuris trichiura Eggs in Human Populations from Thailand, Lao PDR, and Myanmar
    Rutchanee Rodpai, Issarapong Phosuk, Penchom Janwan, Tongjit Thanchomnang, Pewpan M. Intapan, Sakhone Laymanivong, Win Pa Pa Aung, Witthaya Anamnart, Oranuch Sanpool, Adulsak Wijit, Wanchai Maleewong, Lakkhana Sadaow
    The American Journal of Tropical Medicine and Hygiene.2018; 98(1): 39.     CrossRef
  • Strongyloides stercoralis and hookworm co-infection: spatial distribution and determinants in Preah Vihear Province, Cambodia
    Armelle Forrer, Virak Khieu, Fabian Schär, Penelope Vounatsou, Frédérique Chammartin, Hanspeter Marti, Sinuon Muth, Peter Odermatt
    Parasites & Vectors.2018;[Epub]     CrossRef
  • Prevalence of Intestinal Parasites and Associated Risk Factors for Infection among Rural Communities of Chachoengsao Province, Thailand
    Pisit Suntaravitun, Amornrat Dokmaikaw
    The Korean Journal of Parasitology.2018; 56(1): 33.     CrossRef
  • Concurrent infection of a young tourist by hookworm and Strongyloides stercoralis during low budget travel in Southeast Asia
    M. Wesołowska, W. Rymer, M. Kicia, M. Popiołek
    Helminthologia.2018; 55(2): 166.     CrossRef
  • Human liver fluke Opisthorchis viverrini (Trematoda, Opisthorchiidae) in Central Myanmar: New records of adults and metacercariae identified by morphology and molecular analysis
    Oranuch Sanpool, Win Pa Pa Aung, Rutchanee Rodpai, Wanchai Maleewong, Pewpan M. Intapan
    Acta Tropica.2018; 185: 149.     CrossRef
  • First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar
    Win Pa Pa Aung, Thi Thi Htoon, Htay Htay Tin, Kyi Kyi Thinn, Oranuch Sanpool, Jurairat Jongthawin, Lakkhana Sadaow, Issarapong Phosuk, Rutchanee Rodpai, Pewpan M. Intapan, Wanchai Maleewong, Petr Heneberg
    PLOS ONE.2017; 12(5): e0177130.     CrossRef
  • Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children
    Catrin E. Moore, Kristin Elwin, Nget Phot, Chanthou Seng, Saroeun Mao, Kuong Suy, Varun Kumar, Johanna Nader, Rachel Bousfield, Sanuki Perera, J. Wendi Bailey, Nicholas J. Beeching, Nicholas P. J. Day, Christopher M. Parry, Rachel M. Chalmers, James S McC
    PLOS Neglected Tropical Diseases.2016; 10(7): e0004822.     CrossRef
  • Improved Sanitation Facilities are Associated with Higher Body Mass Index and Higher Hemoglobin Concentration Among Rural Cambodian Women in the First Trimester of Pregnancy
    Timothy J. Green, Crystal D. Karakochuk, Judy McLean, Amynah Janmohamed
    The American Journal of Tropical Medicine and Hygiene.2016; 95(5): 1211.     CrossRef
  • A 13-Year Retrospective Study on Primary Liver Cancer in Cambodia: A Strikingly High Hepatitis C Occurrence among Hepatocellular Carcinoma Cases
    François Chassagne, Teresa Rojas Rojas, Stéphane Bertani, Geneviève Bourdy, Sokha Eav, Eloy Ruiz, Pascal Pineau, Eric Deharo
    Oncology.2016; 91(2): 106.     CrossRef
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Fishborne Trematode Metacercariae in Luang Prabang, Khammouane, and Saravane Province, Lao PDR
Han-Jong Rim, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Jong-Yil Chai, Duk-Young Min, Soon-Hyung Lee, Eui-Hyug Hoang, Bounlay Phommasack, Sithat Insisiengmay
Korean J Parasitol 2013;51(1):107-114.
Published online February 18, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.1.107

Fishborne trematode (FBT) metacercariae were investigated in fish from 3 Provinces of Lao PDR. Total 242 freshwater fish of 40 species were collected in local markets of Luang Prabang (59 fish of 16 species), Khammouane (81 fish of 19 species), and Saravane (97 fish of 14 species), and each of them was examined by artificial digestion method. Four species of metacercariae (Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, and Centrocestus formosanus) were detected. O. viverrini was detected in 35 fish (14.5%), and their density was 252 per infected fish (Luang Prabang, 88 metacercariae in 5 fish; Khammouane, 187 in 6 fish; Saravane, 303 in 24 fish). H. taichui was found in 102 fish (42.1%), and their density was 485 per infected fish (Luang Prabang, 260 metacercariae in 38 fish; Khammouane, 1,084 in 23 fish; Saravane, 359 in 41 fish). H. yokogawai was detected in 92 fish (38.0%), and their density was 222 per infected fish (Luang Prabang, 362 metacercariae in 17 fish; Khammouane, 126 in 20 fish; Saravane, 214 in 55 fish). Metacercariae of C. formosanus were found in 8 fish (3.3%), and their density was 3 per infected fish. In the present study, it has been confirmed that FBT metacercariae, in particular, H. taichui, H. yokogawai, and O. viverrini, are highly prevalent in fish from Luang Prabang, Khammouane, and Saravane Province, Lao PDR.

Citations

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  • Prevalence of Foodborne Trematodes in Small Indigenous Fish Species in Local Markets of Phnom Penh, Cambodia
    Domenico Caruso, Lin Kong, Samnang Keo, Sreyni Yoeurn, Samphal Seng
    Vector-Borne and Zoonotic Diseases.2024; 24(3): 141.     CrossRef
  • Re‐evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 2
    Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Lieve Herman, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panag
    EFSA Journal.2024;[Epub]     CrossRef
  • Multiplex PCR development for the simultaneous and rapid detection of two pathogenic flukes, Dactylogyrus spp. and Centrocestus formosanus, in ornamental fishes
    Phonkawin Jaruboonyakorn, Thanawan Tejangkura, Thapana Chontananarth
    Aquaculture.2022; 548: 737660.     CrossRef
  • Prevalence and Risk Factors of Intestinal Helminthiasis in Remote Mountainous Villages of Northern Lao PDR: A Cross-Sectional Study
    Hanna Jin, Kyungshick Ryu, Dajeong Lee, Youthanavanh Vonghachack, Min-Ho Choi, Sung-Tae Hong, Hyun Beom Song
    The Korean Journal of Parasitology.2021; 59(2): 131.     CrossRef
  • Infection Dynamics of Opisthorchis viverrini Metacercariae in Cyprinid Fishes from Two Endemic Areas in Thailand and Lao PDR
    Jutamas Namsanor, Nadda Kiatsopit, Thewarach Laha, Ross H. Andrews, Trevor N. Petney, Paiboon Sithithaworn
    The American Journal of Tropical Medicine and Hygiene.2020; 102(1): 110.     CrossRef
  • Fish-borne trematode infections in wild fishes in Bangladesh
    Sharmin S. Labony, M. Abdul Alim, Muhammad Mehedi Hasan, Md. Shahadat Hossain, Ausraful Islam, Mohammad Zahangir Alam, Naotoshi Tsuji, Anisuzzaman
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  • Genetic Differentiation of Opisthorchis-Like Eggs in Northern Thailand Using Stool Specimens Under National Strategic Plan to Control Liver Fluke Infection and Cholangiocarcinoma
    Saiwasan Buathong, Kateitsaray Phaiphilai, Toon Ruang-areerate, Naruemon Sitthichot, Thanyapit Thita, Mathirut Mungthin, Picha Suwannahitatorn
    The American Journal of Tropical Medicine and Hygiene.2020; 103(3): 1118.     CrossRef
  • Infection status with zoonotic trematode metacercariae in fish from Lao PDR
    Jong-Yil Chai, Soon-Hyung Lee, Han-Jong Rim, Woon-Mok Sohn, Bounlay Phommasack
    Acta Tropica.2019; 199: 105100.     CrossRef
  • Ultrasonographic investigation of cholangiocarcinoma in Lao PDR
    Ju Yeong Kim, Tai-Soon Yong, Han-Jong Rim, Jong-Yil Chai, Duk-Young Min, Keeseon S. Eom, Woon-Mok Sohn, Jae Hoon Lim, Dongil Choi, Sithat Insisiengmay, Bounlay Phommasack, Bounnaloth Insisiengmay
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    Thapana Chontananarth, Sothorn Anucherngchai, Thanawan Tejangkura
    Journal of Parasitic Diseases.2018; 42(3): 423.     CrossRef
  • Mothers’ Opisthorchis viverrini infection status and raw fish dish consumption in Lao People’s Democratic Republic: determinants of child infection status
    Hitomi Araki, Ken Ing Cherng Ong, Lavy Lorphachan, Pheovaly Soundala, Moritoshi Iwagami, Akira Shibanuma, Bouasy Hongvanthong, Paul T. Brey, Shigeyuki Kano, Masamine Jimba
    Tropical Medicine and Health.2018;[Epub]     CrossRef
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Brief Communication

Infection Status of Freshwater Crabs and Crayfish with Metacercariae of Paragonimus westermani in Korea
Eun-Min Kim, Jae-Lip Kim, Sung-Il Choi, Soon-Hyung Lee, Sung-Tae Hong
Korean J Parasitol 2009;47(4):425-426.
Published online December 2, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.4.425

The present study investigated the infection status of Paragonimus westermani metacercariae in freshwater crabs (n = 363) and crayfish (n = 31) from October 2007 to October 2008 using the crush method. All of the freshwater crabs, Eriocheir japonicus, were negative for P. westermani metacercariae while 10 (32.3%) of the 31 examined crayfish were positive. The 10 positive crayfish were caught in Haenam, Jeollanam-do, and there were 8-59 (mean 28.4) metacrcariae per infected crayfish. These results suggest that P. westermani metacerariae are still transmitted by crayfish enzootically in southern Korea, and that freshwater crabs may transmit metacercariae only on rare occasions.

Citations

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  • Survival and infectivity of Paragonimus westermani Metacercariae in soy sauce–marinated crayfish
    Eun-Min Kim, Yan Jin, Sung-Tae Hong
    Food and Waterborne Parasitology.2025; 40: e00277.     CrossRef
  • Inactivation of Paragonimus westermani metacercariae in soy sauce-marinated and frozen freshwater crabs
    Tae Im Kim, Se-Ra Oh, Fuhong Dai, Hyun-Jong Yang, Sang-Do Ha, Sung-Jong Hong
    Parasitology Research.2017; 116(3): 1003.     CrossRef
  • Recent Incidence of Paragonimus westermani Metacercariae in Freshwater Crayfish, Cambaroides similis, from Two Enzootic Sites in Jeollanam-do, Korea
    Jin-Ho Song, Fuhong Dai, Xuelian Bai, Tae-Im Kim, Hyun-Jong Yang, Tong-Soo Kim, Shin-Hyung Cho, Sung-Jong Hong
    The Korean Journal of Parasitology.2017; 55(3): 347.     CrossRef
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    Jia‐Yan Wu, Bao‐Rong Zhang, Guo‐Hua Zhao
    CNS Neuroscience & Therapeutics.2013; 19(9): 734.     CrossRef
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    Ah Young Kim
    The Korean Journal of Gastroenterology.2013; 61(6): 351.     CrossRef
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    Korean Journal of Medicine.2013; 85(5): 469.     CrossRef
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Mini Review

Foodborne Intestinal Flukes in Southeast Asia
Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2009;47(Suppl):S69.
Published online October 23, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.S.S69

In Southeast Asia, a total of 59 species of foodborne intestinal flukes have been known to occur in humans. The largest group is the family Heterophyidae, which constitutes 22 species belonging to 9 genera (Centrocestus, Haplorchis, Heterophyes, Heterophyopsis, Metagonimus, Procerovum, Pygidiopsis, Stellantchasmus, and Stictodora). The next is the family Echinostomatidae, which includes 20 species in 8 genera (Artyfechinostomum, Acanthoparyphium, Echinochasmus, Echinoparyphium, Echinostoma, Episthmium, Euparyphium, and Hypoderaeum). The family Plagiorchiidae follows the next containing 5 species in 1 genus (Plagiorchis). The family Lecithodendriidae includes 3 species in 2 genera (Phaneropsolus and Prosthodendrium). In 9 other families, 1 species in 1 genus each is involved; Cathaemaciidae (Cathaemacia), Fasciolidae (Fasciolopsis), Gastrodiscidae (Gastrodiscoides), Gymnophallidae (Gymnophalloides), Microphallidae (Spelotrema), Neodiplostomidae (Neodiplostomum), Paramphistomatidae (Fischoederius), Psilostomidae (Psilorchis), and Strigeidae (Cotylurus). Various types of foods are sources of human infections. They include freshwater fish, brackish water fish, fresh water snails, brackish water snails (including the oyster), amphibians, terrestrial snakes, aquatic insects, and aquatic plants. The reservoir hosts include various species of mammals or birds.The host-parasite relationships have been studied in Metagonimus yokogawai, Echinostoma hortense, Fasciolopsis buski, Neodiplostomum seoulense, and Gymnophalloides seoi; however, the pathogenicity of each parasite species and host mucosal defense mechanisms are yet poorly understood. Clinical aspects of each parasite infection need more clarification. Differential diagnosis by fecal examination is difficult because of morphological similarity of eggs. Praziquantel is effective for most intestinal fluke infections. Continued efforts to understand epidemiological significance of intestinal fluke infections, with detection of further human cases, are required.

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Original Articles

High Prevalence of Haplorchis taichui, Phaneropsolus molenkampi, and Other Helminth Infections among People in Khammouane Province, Lao PDR
Jong-Yil Chai, Eun-Taek Han, Eun-Hee Shin, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Jin-Young Um, Min-Sung Park, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2009;47(3):243-247.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.243

The prevalence of liver and intestinal helminth infections, including Opisthorchis, Haplorchis, Phaneropsolus, hookworms, Enterobius, and Taenia, was surveyed in Khammouane province, Lao PDR. Fecal specimens were collected from 1,242 people (590 men and 652 women) in 3 Mekong riverside villages and were examined by the Kato-Katz thick smear technique. The overall helminth egg positive rate was 81.1%. The positive rate for small trematode eggs, including Opisthorchis viverrini, heterophyids, and lecithodendriids, was 81.1% and the positive rate for hookworms was 6.7%. To obtain adult worms, 35 people who were positive for small trematode eggs were treated with 20-30 mg/kg praziquantel and 10-15 mg/kg pyrantel pamoate, and then purged. Diarrheic stools were collected from 33 of these people and searched for helminth parasites using a stereomicroscope. Mixed infections with various helminths (Haplorchis taichui, Haplorchis yokogawai, Prosthodendrium molenkampi, Phaneropsolus bonnei, echinostomes, hookworms, Trichostrongylus spp., Trichuris trichiura, Enterobius vermicularis, and/or Taenia saginata) were found. The total number of helminth specimens collected was 20,907 (approximately 634 per person). The most common species was H. taichui, followed by P. molenkampi, O. viverrini, P. bonnei, E. vermicularis, hookworms, and Trichostrongylus spp. These results show that diverse species of intestinal nematodes, trematodes, and cestodes are infecting humans in Khammouane province, Lao PDR.

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A Nationwide Survey on the Prevalence of Intestinal Parasitic Infections in the Republic of Korea, 2004
Tong-Soo Kim, Shin-Hyeong Cho, Sun Huh, Yoon Kong, Woon-Mok Sohn, Seung-Sik Hwang, Jong-Yil Chai, Soon-Hyung Lee, Yun-Kyu Park, Dae-Kyu Oh, Jong-Koo Lee
Korean J Parasitol 2009;47(1):37-47.
Published online March 12, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.1.37

National surveys on the prevalence of intestinal parasitic infections have been carried out every 5-7 years since 1971 in the Republic of Korea in order to establish control measures. The present nationwide survey was conducted from June to December 2004. The 10% population sampling data of Population and Housing Census by the Korean government in 2000 was used as the survey population. One sample was selected randomly from each of the 22,858 registered subjects, and a total of 20,541 people were ultimately included in this survey. Fecal examinations were performed by the cellophane thick smear and saturated brine flotation techniques. Pinworm infection was examined by cello-tape anal swab method. This survey also included a questionnaire study for a socioeconomic analysis. The total helminth egg positive rate was 3.7%, and the estimated total positive number among nationwide people was 1,780,000. The rates in urban and rural areas were 3.1% and 6.8%, respectively. As the total egg positive rate in the 6th survey in 1997 was 2.4%, the present survey showed that there was a considerable degree of increase in the prevalence rate of intestinal parasitic infections over the 7-year period following the 6th survey. The largest increases occurred in the egg positive rates of Clonorchis sinensis and heterophyids including Metagonimus yokogawai.

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Brief Communication

Detection of Gnathostoma spinigerum Third-Stage Larvae in Snakeheads Purchased from a Central Part of Myanmar
Bong-Kwang Jung, Jin-Ju Lee, Kyoung-Ho Pyo, Hyeong-Jin Kim, Hoo-Gn Jeong, Cheong-Ha Yoon, Soon-Hyung Lee, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2008;46(4):285-288.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.285

To examine the infection status of freshwater fish with Gnathostoma spp. larvae in Myanmar, we purchased 15 snakeheads, Channa striatus, from a local market in a suburban area of Naypyidaw, the new capital city. Two larval gnathostomes were collected using an artificial digestion technique, and observed by a light microscope and a scanning electron microscope. The size of an intact larva was 2.65 mm long and 0.32 mm wide. The characteristic morphology of the larvae included the presence of a long esophagus (0.80 mm long), 2 pairs of cervical sacs (0.43 mm long), and a characteristic head bulb with 4 rows of hooklets. The number of hooklets in the 1st, 2nd, 3rd, and 4th row was 45, 48, 50, and 52, respectively. Based on these morphological characters, the larvae were identified as the advanced 3rd-stage larvae of Gnathostoma spinigerum. This is the first report of detection of G. spinigerum 3rd-stage larvae in the central part of Myanmar. Our study suggests that intake of raw meat of snakehead fish in Myanmar may result in human gnathostomiasis.

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Original Articles

Fishborne Trematode Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR
Han-Jong Rim, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Jong-Yil Chai, Duk-Young Min, Soon-Hyung Lee, Eui-Hyug Hoang, Bounlay Phommasack, Sithat Insisengmay
Korean J Parasitol 2008;46(4):253-260.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.253

Freshwater fish from Vientiane Municipality and Savannakhet Province, Lao PDR were examined by the muscle compression and artificial digestion methods to know the infection status with trematode metacercariae. In the fish from Savannakhet, 2 species of metacercariae, Opisthorchis viverrini and Haplorchis taichui, were detected. O. viverrini metacercariae were found in 6 species of fish, Puntius brevis, Hampala dispar, Esomus metallicus, Mystacoleucus marginatus, Puntioplites falcifer, and Cyclocheilichthys armatus. H. taichui metacercariae were detected in 3 species of fish, P. brevis, P. falcifer, and M. marginatus. In the fish from Vientiane, 4 species of metacercariae, O. viverrini, H. taichui, Haplorchis yokogawai, and Centrocestus formosanus, were detected. Among them, O. viverrini metacercariae were found in 7 species of fish, Onychostoma elongatum, C. armatus, H. dispar, P. brevis, Cyclocheilichthys repasson, Osteochilus hasseltii, and Hypsibarbus lagleri. The metacercariae of H. taichui were detected in 6 species of fish, C. repasson, O. elongatum, C. armatus, H. dispar, Labiobarbus leptocheila, and Cirrhinus molitorella. The metacercariae of H. yokogawai were found in 9 species of fish, C. repasson, O. elongatum, C. armatus, H. dispar, Labiobarbus leptocheila, O. hasseltii. C. molitorella, Hypsibarbus wetmorei, and H. lagleri. The metacercariae of C. formosanus were detected in 4 species of fish, C. repasson, P. brevis, O. hasseltii, and C. molitorella. From these results, it is confirmed that fishborne trematode metacercariae, i.e. O. viverrini, H. taichui, H. yokogawai and C. formosanus, are prevalent in various species of freshwater fish from Savannakhet Province and Vientiane Municipality, Lao PDR.

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Infection Status of Freshwater Fish with Metacercariae of Clonorchis sinensis in Korea
Eun-Min Kim, Jae-Lip Kim, Sung Yil Choi, Jae-Whan Kim, Siwon Kim, Min-Ho Choi, Young Mee Bae, Soon-Hyung Lee, Sung-Tae Hong
Korean J Parasitol 2008;46(4):247-251.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.247

This study investigated freshwater fish for their current infection status with metacercariae of Clonorchis sinensis in Korea. Twenty-one species of freshwater fish (n = 677) were collected from 34 regions nationwidely from February 2007 to June 2008. They were individually examined by digestion technique. Eight species of freshwater fish from 17 different regions were recognized positive for the metacercariae of C. sinensis. The positive rates (range of metacercariae number per fish) of fish by the species were as follows: 48% (1-1,142) in Pseudorasbora parva, 60% (1-412) in Pungtungia herzi, 15.7% (1-23) in Pseudogobio esocinus, 29% (1-7) in Acheilognathus intermedia, 21% (1-4) in Odontobutis interrupta, 33% (1-6) in Zacco temmincki, 3.6% (1-4) in Zacco platypus, and 26.3% (1) in Hemibarbus labeo. The two species, P. parva and P. herzi, are able to be the index fish for estimation of C. sinensis transmission in a certain locality. Still several species of freshwater fish are briskly transmitting C. sinensis infection in many riverside areas of southern Korea.

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High prevalence of liver and intestinal fluke infections among residents of Savannakhet Province in Laos
Jong-Yil Chai, Eun-Taek Han, Sang-Mee Guk, Eun-Hee Shin, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Keon-Hoon Lee, Hoo-Gn Jeong, Yong-Sang Ryang, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2007;45(3):213-218.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.213

The prevalence of liver and intestinal fluke infections was surveyed on residents of Savannakhet Province, Laos. Fecal specimens were collected from a total of 981 residents in 4 Mekong riverside villages and examined by the Kato-Katz thick smear technique. The results revealed that the overall helminth egg positive rate was 84.2%, and the positive rate for small trematode eggs, including Opisthorchis viverrini, heterophyids, or lecithodendriids, was 67.1%. To obtain adult flukes, 38 small trematode egg positive cases were treated with a 20-30 mg/kg single dose of praziquantel and purged. Diarrheic stools were then collected from 29 people and searched for helminth parasites using stereomicroscopes. Mixed infections with O. viverrini and 6 kinds of intestinal flukes were found, namely, Haplorchis taichui, Haplorchis pumilio, Haplorchis yokogawai, Prosthodendrium molenkampi, Phaneropsolus bonnei, and echinostomes. The total number of flukes collected was 7,693 specimens (av. no. per treated person; 265.3). The most common species was O. viverrini, followed by H. taichui, P. molenkampi, echinostomes, H. pumilio, P. bonnei, and H. yokogawai. The results indicate that foodborne liver and intestinal fluke infections are prevalent among residents of Savannakhet Province, Laos.

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A survey of Heterophyes nocens and Pygidiopsis summa metacercariae in mullets and gobies along the coastal areas of the Republic of Korea
Sang-Mee Guk, Eun-Hee Shin, Jae-Lip Kim, Woon-Mok Sohn, Kwang-Sun Hong, Cheong-Ha Yoon, Soon-Hyung Lee, Han-Jong Rim, Jong-Yil Chai
Korean J Parasitol 2007;45(3):205-211.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.205

The infection status of mullets Mugil cephalus (n = 139) and gobies (n = 35) Acanthogobius flavimanus with metacercariae of Heterophyes nocens and Pygidiopsis summa was examined in 11 western, southern, and eastern coastal areas of the Republic of Korea, using a digestion technique. Heterophyid metacercariae were highly prevalent in mullets from western and southern coastal areas; Shinan-gun (100% for H. nocens and 100% for P. summa), Muan-gun (93% and 100%), Buan-gun (42% and 75%), Seocheon-gun (73% and 53%), Ganghwa-gun (47% and 100%), Sacheon-shi (47% and 77%), and Gangjin-gun (50% and 70%, respectively). Only 1 (10%) of 10 mullets from an eastern coastal area, i.e., Donghae-shi, was positive for P. summa metacercariae. Metacercarial densities were the highest in the trunk of mullets for H. nocens and the gill for P. summa. Gobies from Muan-gun were positive for H. nocens (40%) and P. summa metacercariae (40%), and gobies from Seocheon-gun revealed H. nocens metacercariae (20%). The metacercarial density was remarkably higher in mullets than in gobies. The results revealed that H. nocens and P. summa metacercariae are prevalent in mullets and gobies from coastal areas of the Republic of Korea, and the prevalence and intensity of infection vary according to geographical locality.

Citations

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  • Infection characteristics of Metagonimus species (Digenea: Heterophyidae) metacercariae in fish from major rivers of Korea
    Woon-Mok Sohn
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    The Korean Journal of Parasitology.2018; 56(2): 183.     CrossRef
  • Infection Status with Metagonimus spp. Metacercariae in Fishes from Seomjin-gang and Tamjin-gang in Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Cheon-Hyeon Kim, Ki-Bok Yoon
    The Korean Journal of Parasitology.2018; 56(4): 351.     CrossRef
  • Infections with Zoonotic Trematode Metacercariae in Yellowfin Goby, Acanthogobius flavimanus, from Coastal Areas of Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Soon-Won Lee, Won-Seok Seok
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  • Pygidiopsis summa (Digenea: Heterophyidae): Status of Metacercarial Infection in Mullets from Coastal Areas in the Republic of Korea
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    The Korean Journal of Parasitology.2016; 54(4): 497.     CrossRef
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  • Prevalence of Zoonotic Metacercariae in Two Species of Grouper, Epinephelus coioides and Epinephelus bleekeri, and Flathead Mullet, Mugil cephalus, in Vietnam
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Parvatrema chaii n. sp. (Digenea: Gymnophallidae) from mice experimentally infected with metacercariae collected from surf-clam, Mactra veneriformis
Woon-Mok Sohn, Byoung-Kuk Na, Yong-Suk Ryang, Hilda Lei Ching, Soon-Hyung Lee
Korean J Parasitol 2007;45(2):115-120.
Published online June 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.2.115

Parvatrema chaii n. sp. (Digenea: Gymnophallidae) is described using the worms recovered from experimentally infected mice in Korea. The metacercariae were collected from surf-clams, Mactra veneriformis, from a tidal flat in Sochon-gun, Chungchongnam-do. The metacercariae were elliptical (0.262 × 0.132 mm), and the genital pore had an anterior arch of 16-17 sensory papillae in scanning electron microscopic view. Adult worms were ovoid to foliate (0.275-0.303 by 0.140-0.150 mm), and their characteristic features included the presence of lateral lips, short esophagus, genital pore located some distance anterior to the ventral sucker, club-shaped seminal vesicle, a compact to slightly lobed vitellarium, elliptical eggs (0.018-0.020 by 0.010-0.013 mm), and absence of the ventral pit. This gymnophallid is classified as a member of the genus Parvatrema because of the location of the wide genital pore some distance from the ventral sucker, and the absence of the ventral pit. It differs from previously reported Parvatrema species, including the type species, P. borinquenae. In particular, the morphologies of the vitellarium and the genital pore with an anterior arch of 16-17 sensory papillae are unique features. Therefore, we propose it as a new species, Parvatrema chaii n. sp. (Digenea: Gymnophallidae).

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Brief Communications

Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence
Tai-Soon Yong, Jong-Ho Lee, Seobo Sim, Jongweon Lee, Duk-Young Min, Jong-Yil Chai, Keeseon S. Eom, Woon-Mok Sohn, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2007;45(1):69-74.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.69

Trichostrongylus eggs observed in cellophane-thick smears are difficult, in practice, to distinguish from hookworm eggs. In order to overcome these limitations, a molecular approach was conducted. A Trichostrongylus colubriformis adult worm was obtained from a human in Laos, which was identified morphologically. ITS-1 sequence of this worm was determined, and found to be most similar with that of T. colubriformis among the Trichostrongylus spp. reported so far. Then, this sequence was compared with those of human hookworm species, Ancylostoma duodenale and Necator americanus, and species-specific oligonucleotide primers were designed. Polymerase chain reaction (PCR) using these primers evidenced specifically amplified PCR products of Trichostrongylus sp., A. duodenale and N. americanus from the eggs of each (520 bp, 690 bp, and 870 bp, respectively). A species-specific PCR technique can be developed in order to study the epidemiology of Trichostrongylus spp. and hookworms in endemic areas.

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A survey of cryptosporidiosis among 2,541 residents of 25 coastal islands in Jeollanam-do (Province), Republic of Korea
Jae-Hwan Park, Hyo-Jin Kim, Sang-Mee Guk, Eun-Hee Shin, Jae-Lip Kim, Han-Jong Rim, Soon-Hyung Lee, Jong-Yil Chai
Korean J Parasitol 2006;44(4):367-372.
Published online December 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.4.367

In order to determine the distribution and prevalence of human cryptosporidiosis on western and southern coastal islands of Jeollanam-do (Province), fecal samples were collected from 2,541 people residing on 25 islands, 13 in the western coasts and 12 in the southern coasts, during July and August 2000. Fecal smears were prepared following formalin-ether sedimentation of the samples and stained by a modified acid-fast procedure. The presence of Cryptosporidium oocysts was determined by light microscopy. Cryptosporidium oocysts were detected in 38 specimens (1.5%). The oocyst positive rate varied (0-6.0%) according to island; the highest was detected on Oenarodo (6.0%), followed by Naenarodo (5.6%) and Nakwoldo (5.4%). The majority (35 persons, 94.6%) of Cryptosporidium-infected individuals were older than 50 years of age. Men (22/1,159; 1.9%) were infected at a higher rate than women (16/1,382; 1.2%). The results of the present survey indicate that human Cryptosporidium infections (due to Cryptosporidium hominis and/or C. parvum) are maintained at a relatively low prevalence on coastal islands of Jeollanam-do, Republic of Korea.

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Mini Review

A successful experience of soil-transmitted helminth control in the Republic of Korea
Sung-Tae Hong, Jong-Yil Chai, Min-Ho Choi, Sun Huh, Han-Jong Rim, Soon-Hyung Lee
Korean J Parasitol 2006;44(3):177-185.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.177

Soil-transmitted helminths (STH), namely Ascaris, Trichuris and hookworms (Ancylostoma and Necator), present a global health problem to about a half of the earth's population. In the Republic of Korea, STH were highly prevalent and were considered a high priority target for national control. To promote the control, a non-governmental organization named Korea Association for Parasite Eradication (currently Korea Association of Health Promotion) was founded in 1964, and mass fecal examination followed by selective mass chemotherapy with anthelmintics was performed twice a year from 1969 to 1995 targeting whole nationwide schoolchildren. Meanwhile, decreasing patterns of national STH infections have been monitored by 7 times' quinquennial national surveys targeting general population. In 1971, the overall intestinal helminth egg positive rate was 84.3% (Ascaris 58.2%, Trichuris 65.4%, and hookworms 10.7%), which became 63.2% in 1976, 41.1% in 1981, 12.9% in 1986, 3.8% in 1992, 2.4% in 1997, and 4.3% (Ascaris 0.03%, Trichuris 0.02%, and hookworms 0%) in 2004. During the control period, national economy rapidly developed, and living standards including environment, sanitation, and agricultural technology greatly improved, which undoubtedly boosted the STH control effects. Our experience indicates that social driving force to establish an eligible national control system to conduct repeated mass chemotherapy, together with improvement of environment and sanitation, is important for initiating and achieving STH control in a developing community.

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Original Articles

Bile duct changes in rats reinfected with Clonorchis sinensis
Dongil Choi, Sung-Tae Hong, Shunyu Li, Byung-Suk Chung, Jae Hoon Lim, Soon-Hyung Lee
Korean J Parasitol 2004;42(1):7-17.
Published online March 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.1.7

This study describes an evaluation of the sonographic, cholangiographic, pathological, and immunological findings, and the protective effect shown by rats reinfected with Clonorchis sinensis. Eight experimental rat groups were, namely, a normal control, a primary infection control, a reinfection I (reinfection 7 week after treatment following 3-week infection), a reinfection II (reinfection 2 week after treatment following 8-week infection), a reinfection III (exploration of the intrahepatic bile ducts 1 week after reinfection 4 week after treatment following 4-week infection), a superinfection, a secondary infection control, and an infection following immunization group. Sonographic and cholangiographic findings showed moderate or marked dilatation of the bile duct confluence in the primary infection control, reinfection II, and secondary infection control groups. Juvenile worms survived in the intrahepatic bile ducts 1 week after reinfection following treatment in the reinfection III group. It was concluded that reinfecting juvenile worms found during the first week following reinfection failed to survive or grow further. Anatomical, pathophysiological, or immunological changes may induce protection from reinfection in rats.

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Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea
Jong-Yil Chai, Aifen Lin, Eun-Hee Shin, Myoung-Don Oh, Eun-Taek Han, Ho-Woo Nam, Soon-Hyung Lee
Korean J Parasitol 2003;41(3):147-154.
Published online September 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.3.147

Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a well-known virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea.

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    Eun-Hee Shin, Il-Myung Kim, Jae-Lip Kim, Eun-Taek Han, Yun-Kyu Park, Yukifumi Nawa, Jina Kook, Soon-Hyung Lee, Jong-Yil Chai
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Brief Communication

A survey of Brugia malayi infection on the Heugsan Islands, Korea
Jong-Yil Chai, Soon-Hyung Lee, Sung-Yil Choi, Jong-Soo Lee, Tai-Soon Yong, Kyun-Jong Park, Kyeong-Ae Yang, Keon-Hoon Lee, Mi-Jeng Park, Hyung-Ran Park, Mi-Ja Kim, Han-Jong Rim
Korean J Parasitol 2003;41(1):69-73.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.69

Lymphatic filariasis due to Brugia malayi infection was endemic in several areas of South Korea. The infection was controlled, or disappeared, in most areas, with the exception of the remote southwestern islands of Jeonranam-do, including the Heugsan Islands. To discover its current situation, a small-scale survey was performed on the Heugsan Islands in September 2000. A total of 378 people, 151 male and 227 female, living in 8 villages (6 on Daeheugsan-do, 1 on Daejang-do, and 1 on Yeongsan-do) were subjected to a night blood survey for microfilaremia, and physical examination for elephantiasis on the extremities. There were 6 (1.6%) microfilaria positive cases, all in females aged 57-72 years, and from only two villages of the Daeheugsan-do area. There were 4 patients with lower leg elephantiasis, but they showed no microfilaremia. The results show that a low-grade endemicity of filariasis remains on the Daeheugsan-do.

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Original Article

Surface ultrastructure of Pygidiopsis summa (Digenea: Heterophyidae) adult flukes
Jong-Yil Chai, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee
Korean J Parasitol 2002;40(3):107-112.
Published online September 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.3.107

A scanning electron microscopic study was performed on the surface ultrastructure of Pygidiopsis summa (Digenea: Heterophyidae) adults. Metacercariae were collected from gills and muscles of mullets (Mugil cephalus) caught in a known endemic area, and adult flukes were harvested from dogs after 8 weeks of experimental infection. The worm was calabash form with its posterior part broader than the anterior part. Tegumental spines were densely distributed over the body surface, except on the suckers and genital apparatus, and around the excretory pore. Well differentiated spines were observed on the anterior half of the body, with 14-16 tips ventrally, and 19-20 tips dorsally. On the oral sucker, three pairs of type I sensory papillae (uni-ciliated knob-like swellings) and one pair of type II sensory papillae (aciliated round-swellings) were observed on the anterior and posterior parts of the lip, respectively. On the lip of the ventral sucker, one pair of type II sensory papillae was distributed only on its posterior part. Sperms were seen emerging from or entering into the genital apparatus. The results showed that the surface ultrastructure of P. summa was unique among the heterophyid trematodes, especially in digitation of tegumental spines and in distribution of sensory papillae on oral and ventral suckers.

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  • REDESCRIPTION AND SURFACE ULTRASTRUCTURE OF PYGIDIOPSIS MACROSTOMUM (DIGENEA: HETEROPHYIDAE)
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Mini Review

A review of Gymnophalloides seoi (Digenea: Gymnophallidae) and human infections in the Republic of Korea
Soon-Hyung Lee, Jong-Yil Chai
Korean J Parasitol 2001;39(2):85-118.
Published online June 30, 2002
DOI: https://doi.org/10.3347/kjp.2001.39.2.85

Studies on Gymnophalloides seoi (Digenea: Gymnophallidae) and human infections are briefly reviewed. This minute intestinal fluke was first discovered from a Korean woman suffering from acute pancreatitis and gastrointestinal troubles. It was described as a new species by Lee, Chai and Hong in 1993. The southwestern coastal village where the patient resided was found to be a highly endemic area, and additional endemic areas have been identified. The parasite is very small, 0.33-0.50 mm long and 0.23-0.33 mm wide, and characterized by the presence of a ventral pit. The first intermediate host remains unknown, but the second intermediate host has been found to be the oyster Crassostrea gigas. Man and the Palearctic oystercatcher Haematopus ostralegus have been shown to be natural definitive hosts, and wading birds including the Kentish plover Charadrius alexandrinus are highly susceptible to experimental infection. Gerbils, hamsters, cats, and several strains of mice were also susceptible laboratory hosts. In experimentally infected mice, the parasites inhabit the small intestine, pinching and sucking the root of villi with their large oral suckers, but they did not invade beyond the mucosa in immunocompetent mice. However, they were found to invade the submucosa in immunosuppressed mice. Human G. seoi infections have been found in at least 25 localities; 23 islands on the Yellow Sea or the South Sea, and 2 western coastal villages. The highest prevalence was found in a village on Aphaedo, Shinan-gun (49% egg positive rate); other areas showed 0.8-25.3% prevalence. Infected people complained of variable degrees of gastrointestinal troubles and indigestion. The infection can be diagnosed by recovery of eggs in the feces; however, an expert is needed to identify the eggs. Praziquantel, 10 mg/kg in single dose, is effective for treatment of human infections. Eating raw oysters in endemic areas should be avoided.

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    Jong-Yil Chai
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Original Articles

Control of clonorchiasis by repeated treatments with praziquantel
Sung-Tae Hong, Han-Jong Rim, Duk-Young Min, Xueming Li, Jingxian Xu, Zheng Feng, Soon-Hyung Lee
Korean J Parasitol 2001;39(4):285-292.
Published online December 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.4.285

The present study aimed to evaluate control efficacy of clonorchiasis by two schemes of repeated treatment with praziquantel at two endemic villages In China. Residents of one village at Guangxi Autonomous Region were treated and examined 6-monthly and of another at Liaoning Province 12-monthly. In residents that took 25 mg/kg x3 (total 75 mg/kg) of praziquantel every 6 months for one year the egg positive rate showed a significant drop from 69.0% to 17.1%. In contrast, a group of same praziquantel medication once showed a slight marginal decrease in the egg rate from 18.9% to 12.2% after one year. Of 39 subjects examined 3 times, 56.4% were cured, 7.7% persistently positive, one (2.6%) reinfected after cure or newly infected, but 25.6% were persistently negative. The present finding suggests that 6-monthly medication with 75 mg/kg of praziquantel should effectively lower the prevalence but incomplete for control of clonorchiasis in heavy endemic areas.

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Experimental infection of Anopheles sinensis with Korean isolates of Plasmodium vivax
Hyeong Woo Lee, Shin Hyeong Cho, E-Hyun Shin, Jong Soo Lee, Joon-Sang Lee, Jong-Yil Chai, Soon-Hyung Lee, Tong Soo Kim
Korean J Parasitol 2001;39(2):177-183.
Published online June 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.2.177

The
objective
s of the present study were to (1) determine the susceptibility of Anopheles sinensis to Korean isolates of Plasmodium vivax, (2) establish a method to collect large quantities of P. vivax sporozoites for use as antigen in seroepidemiological studies, and (3) investigate the characteristics of Korean isolates of P. vivax sporozoites. Females of Anopheles sinensis were collected at non-epidemic area, Seokwha-ri, Cheongwon-gun and Chungcheongbuk-do using tent-trap methods coupled with dry ice. The females were artificially infected with gametocytes of P. vivax using blood obtained from P. vivax malaria patients. Individual mosquitoes were infected using either a parafilm-covered glass feeding apparatus or were allowed to feed on naturally infected volunteers. Mosquitoes were sacrificed between 16 and 18 days post-feeding and an enzyme-linked immunosorbent assay (ELISA) was used to detect sporozoites. Four (33.4%) of 12 mosquitoes, which were fed on naturally infected volunteers directly, were positive for sporozoites. In cases, the mosquitoes allowed to feed on whole blood which were extract from three different patients with heparin treated vacuutainers using a parafilm-covered glass apparatus. Two of 55 (3.6%) were positive which blood sample was maintained at room temperature for 8 hours, 1 of 68 (1.5%) was positive which blood was maintained at 4℃ for 24 hours and 1 of 47 (2.3%) was positive at 4℃ for 48 hours. The mean number of sporozoites was estimated about 818 (n=8; range of 648-1,056) based on optical density values of ELISA.

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    Won-Ja Lee, Terry A. Klein, Heung-Chul Kim, Young-Mi Choi, Seok-Hyun Yoon, Kyu-Sik Chang, Sung-Tae Chong, In-Yong Lee, James W. Jones, Jolanta S. Jacobs, Jetsumon Sattabongkot, Jae-Sun Park
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  • Detection of vivax sporozoites naturally infected in Anopheline mosquitoes from endemic areas of northern parts of Gyeonggi-do (province) in Korea
    Hyeong Woo Lee, E Hyun Shin, Shin Hyeong Cho, Hee Il Lee, Chung Lim Kim, Wook Gyo Lee, Sung Ung Moon, Jong Soo Lee, Wan Ja Lee, Tong Soo Kim
    The Korean Journal of Parasitology.2002; 40(2): 75.     CrossRef
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    E-Hyun Shin, Tong-Soo Kim, Hyeong-Woo Lee, Jong-Soo Lee, Won-Ja Lee
    The Korean Journal of Parasitology.2002; 40(1): 41.     CrossRef
  • Intraspecific Hybridization of Anopheles sinensis (Diptera: Culicidae) Strains from Thailand and Korea
    Gi-Sik Min, Wej Choochote, Atchariya Jitpakdi, Se-Joo Kim, Won Kim, Jongwoo Jung, Anuluck Junkum
    Molecules and Cells.2002; 14(2): 198.     CrossRef
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Intestinal histopathology and in situ postures of Gymnophalloides seoi in experimentally infected mice
Jong-Yil Chai, Hong-Soon Lee, Sung-Jong Hong, Jae Hyung Yoo, Sang-Mee Guk, Min Seo, Min-Ho Choi, Soon-Hyung Lee
Korean J Parasitol 2001;39(1):31-41.
Published online March 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.1.31

The intestinal histopathology and in situ postures of Gymnophalloides seoi (Digenea: Gymnophallidae) were studied using C3H/HeN and C57BL/6 mice as experimental hosts; the effects of immunosuppression were also observed. The metacercariae isolated from naturally infected oysters, 300 or 1,000 in number, were infected orally to each mouse, and the mice were killed at days 3-21 post-infection (PI). In immunocompetent (IC) mice, only a small number of flukes were found in the mucosa of the duodenum and jejunum during days 3-7 PI, with their large oral suckers pinching and sucking the root of villi. The intestinal mucosa showed mild villous atrophy, crypt hyperplasia, and inflammations in the villous stroma and crypt, with remarkable goblet cell hyperplasia. These mucosal changes were almost restored after days 14-21 PI. In immunosuppressed (IS) mice, displacement as well as complete loss of villi adjacent to the flukes was frequently encountered, otherwise the histopathology was generally mild, with minimal goblet cell hyperplasia. In these mice, numerous flukes were found, and it seemed that they were actively moving and rotating in situ. Several flukes were found to have invaded into the submucosa, almost facing the serosa. These results indicate that in IC mice the intestinal histopathology caused by G. seoi is generally mild, and the flukes do not penetrate beyond the mucosa, however, in IS mice, the flukes can cause severe destruction of neighboring villi, and some of them invade into the submucosa.

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    Asmaa M. I. Abuzeid, Mahmoud M. Hefni, Yue Huang, Long He, Tingting Zhuang, Guoqing Li
    Frontiers in Veterinary Science.2022;[Epub]     CrossRef
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    Olfat A. Mahdy, Sahar Z. Abdel‐Maogood, Mohamed Abdelsalam, Mohamed Shaalan, Hisham A. Abdelrahman, Mai A. Salem
    Aquaculture Research.2021; 52(9): 4475.     CrossRef
  • Foodborne Intestinal Flukes in Southeast Asia
    Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • A new endemic focus of Gymnophalloides seoi infection on Aphae Island, Shinan-gun, Jeollanam-do
    Jae-Hwan Park, Sang-Mee Guk, Eun-Hee Shin, Hyo-Jin Kim, Jae-Lip Kim, Min Seo, Yun-Kyu Park, Jong-Yil Chai
    The Korean Journal of Parasitology.2007; 45(1): 39.     CrossRef
  • A case of colonic lymphoid tissue invasion by Gymnophalloides seoi in a Korean man
    Min Seo, Hokyung Chun, Geunghwan Ahn, Kee-Taek Jang, Sang-Mee Guk, Jong-Yil Chai
    The Korean Journal of Parasitology.2006; 44(1): 87.     CrossRef
  • Gymnophalloides seoi: a new human intestinal trematode
    Jong-Yil Chai, Min-Ho Choi, Jae-Ran Yu, Soon-Hyung Lee
    Trends in Parasitology.2003; 19(3): 109.     CrossRef
  • Food-borne intestinal trematode infections in the Republic of Korea
    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
  • A review of Gymnophalloides seoi (Digenea: Gymnophallidae) and human infections in the Republic of Korea
    Soon-Hyung Lee, Jong-Yil Chai
    The Korean Journal of Parasitology.2001; 39(2): 85.     CrossRef
  • 9,308 View
  • 75 Download
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A nationwide survey of the prevalence of human Gymnophalloides seoi infection on western and southern coastal islands in the Republic of Korea
Jong-Yil Chai, Jae-Hwan Park, Eun-Taek Han, Eun-Hee Shin, Jae-Lip Kim, Kwang-Seon Hong, Han-Jong Rim, Soon-Hyung Lee
Korean J Parasitol 2001;39(1):23-30.
Published online March 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.1.23

A nationwide survey was performed to know the distribution and prevalence of human Gymnophalloides seoi infection on western and southern coastal islands in the Republic of Korea. A total of 4,178 fecal specimens were collected from residents on 45 (24 western and 21 southern) islands, and examined by Kato-Katz and formalin-ether sedimentation techniques. Eggs of G. seoi were detected from 160 (3.8%) people living on 22 (13 western and 9 southern) islands. The prevalence varied by the location of islands; higher on western islands than on southern islands. The highest prevalence was found on Amtaedo (25.3%), followed by Cheungdo (25.0%), and Anchwado (20.9%) (Shinan-gun). A little lower prevalence was observed on Munyodo (13.3%), Shinshido (12.9%), and Sonyudo (10.3%) (Kunsan-shi). Of the remaining islands, the regions showing the prevalence greater than 5% included Kohado, Dallido (Mokpo-shi), Pyeongildo, Kogumdo (Wando-gun), and Keogumdo (Kohung-gun). A strong age predilection was noted (p < 0.05); 95% of the infected people were over 40 years old. Females showed a little higher prevalence than males. The results indicate that human G. seoi infection is more widely distributed than previously considered. Nine of 11 islands (excluding the 2 known areas Munyodo and Sunyudo) that showed greater prevalence than 5% are regarded as new endemic foci of G. seoi.

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    Langui Song, Qingxing Xie, Zhiyue Lv
    Asian Pacific Journal of Tropical Medicine.2021; 14(9): 385.     CrossRef
  • A potential zoonotic parasite, the digenean Gymnophalloides nacellae, on the Magellanic coast in the Southwestern Atlantic Ocean: its life cycle and geographical distribution
    Carmen Gilardoni, Gisele Di Giorgio, Estefanía Bagnato, Susana Pina, Pedro Rodrigues, Florencia Cremonte
    Polar Biology.2020; 43(6): 725.     CrossRef
  • Survey of Gymnophalloides seoi Metacercariae in Natural and Cultured Oysters from Several Western Coastal Areas, Korea
    Taehee Chang, Bong-Kwang Jung, Hyemi Song, Jaeeun Cho, Sooji Hong, Keon-Hoon Lee, Eui-Hyug Hoang, Jisu Kang, Jini Lim, Hana Lee, Jong-Yil Chai
    The Korean Journal of Parasitology.2019; 57(6): 705.     CrossRef
  • Prevalence and Density of Digenetic Trematode Metacercariae in Clams and Oysters from Western Coastal Regions of the Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Won-Ja Lee
    The Korean Journal of Parasitology.2017; 55(4): 399.     CrossRef
  • Recovery of Oswaldotrema nacinovici from Whimbrels (Aves) in Korea
    Young-Il Lee, Ok-Sik Chung, Min Seo
    The Korean Journal of Parasitology.2016; 54(6): 809.     CrossRef
  • Paleoparasitological Studies on Mummies of the Joseon Dynasty, Korea
    Min Seo, Adauto Araujo, Karl Reinhard, Jong Yil Chai, Dong Hoon Shin
    The Korean Journal of Parasitology.2014; 52(3): 235.     CrossRef
  • Increased Intestinal Epithelial Cell Turnover and Intestinal Motility in Gymnophalloides seoi-Infected C57BL/6 Mice
    Sang Hyub Lee, Bong-Kwang Jung, Jae-Hwan Park, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2014; 52(3): 273.     CrossRef
  • A Survey of Intestinal Parasite Infection during a 10-Year Period (2003-2012)
    Young-Eun Kim, Hee Jae Huh, Yu-Yean Hwang, Nam Yong Lee
    Annals of Clinical Microbiology.2013; 16(3): 134.     CrossRef
  • Sixteenth CenturyGymnophalloides seoiInfection on the Coast of the Korean Peninsula
    Dong Hoon Shin, Chang Seok Oh, Jong-Yil Chai, Min Ju Ji, Hye-Jung Lee, Min Seo
    Journal of Parasitology.2012; 98(6): 1283.     CrossRef
  • Foodborne Intestinal Flukes in Southeast Asia
    Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • Trends in parasitic diseases in the Republic of Korea
    Eun-Hee Shin, Sang-Mee Guk, Hyo-Jin Kim, Soon-Hyung Lee, Jong-Yil Chai
    Trends in Parasitology.2008; 24(3): 143.     CrossRef
  • Gymnophalloides seoi Eggs from the Stool of a 17th Century Female Mummy Found in Hadong, Republic of Korea
    Min Seo, Dong Hoon Shin, Sang-Mee Guk, Chang Seok Oh, Eun-Joo Lee, Myung Ho Shin, Myeung Ju Kim, Soong Deok Lee, Yi-Suk Kim, Yang Su Yi, Mark Spigelman, Jong-Yil Chai
    Journal of Parasitology.2008; 94(2): 467.     CrossRef
  • A new endemic focus of Gymnophalloides seoi infection on Aphae Island, Shinan-gun, Jeollanam-do
    Jae-Hwan Park, Sang-Mee Guk, Eun-Hee Shin, Hyo-Jin Kim, Jae-Lip Kim, Min Seo, Yun-Kyu Park, Jong-Yil Chai
    The Korean Journal of Parasitology.2007; 45(1): 39.     CrossRef
  • Prevalence of Gymnophalloides seoi infection in coastal villages of Haenam-gun and Yeongam-gun, Republic of Korea
    Sang-Mee Guk, Jae-Hwan Park, Eun-Hee Shin, Jae-Lip Kim, Aifen Lin, Jong-Yil Chai
    The Korean Journal of Parasitology.2006; 44(1): 1.     CrossRef
  • A survey of Enterobius vermicularis infection among children on western and southern coastal islands of the Republic of Korea
    Jae-Hwan Park, Eun-Taek Han, Won-Hee Kim, Eun-Hee Shin, Sang-Mee Guk, Jae-Lip Kim, Jong-Yil Chai
    The Korean Journal of Parasitology.2005; 43(4): 129.     CrossRef
  • Gymnophalloides seoi: a new human intestinal trematode
    Jong-Yil Chai, Min-Ho Choi, Jae-Ran Yu, Soon-Hyung Lee
    Trends in Parasitology.2003; 19(3): 109.     CrossRef
  • Food-borne intestinal trematode infections in the Republic of Korea
    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
  • A review of Gymnophalloides seoi (Digenea: Gymnophallidae) and human infections in the Republic of Korea
    Soon-Hyung Lee, Jong-Yil Chai
    The Korean Journal of Parasitology.2001; 39(2): 85.     CrossRef
  • 10,346 View
  • 99 Download
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Localization of cytoskeletal proteins in Pneumocystis carinii by immuno-electron microscopy
Jae-Ran Yu, Jae-Kyong Pyon, Min Seo, Byung-Suk Jung, Sang Rock Cho, Soon-Hyung Lee, Sung-Tae Hong
Korean J Parasitol 2001;39(1):13-21.
Published online March 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.1.13

Pneumocystis carinii causes serious pulmonary infection in immunosuppressed patients. This study was undertaken to observe the cytoskeletal proteins of P. carinii by immuno-electron microscopy. P. carinii infection was experimentally induced by immunosuppression of Sprague-Dawley rats for seven weeks, and their lungs were used for the observations of this study. The gold particles localized actin, tropomyosin, and tubulin. The actin was irregularly scattered in the cytoplasm of the trophic forms but was much more concentrated in the inner space of the cell wall of the cystic forms called the inner electron-lucent layer. No significant amount of tropomyosin was observed in either trophic forms or cystic forms. The tubulin was distributed along the peripheral cytoplasm and filopodia of both the trophic and cystic forms rather than in the inner side of the cytoplasm. Particularly, in the cystic forms, the amount of tubulin was increased and located mainly in the inner electron-lucent layer of the cell wall where the actin was concentrated as well. The results of this study showed that the cell wall of P. carinii cystic forms is a structure whose inner side is rich in actin and tubulin. The location of the actin and tubulin in P. carinii suggests that the main role of these proteins is an involvement in the protection of cystic forms from the outside environment by maintaining rigidity of the cystic forms.

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  • A Molecular Window into the Biology and Epidemiology of Pneumocystis spp
    Liang Ma, Ousmane H. Cissé, Joseph A. Kovacs
    Clinical Microbiology Reviews.2018;[Epub]     CrossRef
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  • 78 Download
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Genetic heterogeneity of Pneumocystis carinii from rats of several regions and strains
Byung-Suk Chung, Yun-Kyu Pars, Sun Huh, Jae-Ran Yu, Jin Kim, Xiaohua Shi, Sang Rock Cho, Soon-Hyung Lee, Sung-Tae Hong
Korean J Parasitol 2000;38(3):151-158.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.151

Pneumocystis carinii is a major opportunistic pathogen which has been found in the lungs of a wide variety of mammalian host species, and the fact suggests the possibility of intraspecific variation. Until now, P. carinii from different mammalian species are differentiated as subspecies, and the rats are known to be infected by two subspecies. The present study investigated genetic heterogeneity of P. carinii isolates from two strains of rats in Korea and China by molecular karyotyping, RFLP and sequencing analysis. Karyotypes of P. carinii were grouped into three, two from two strains of rats in Korea and one from rats in China. However RFLP of PCR product of ribosomal and MSG gene of the P. carinii isolates showed same pattern. The sequence homology rates of α-tubulin DNA of the P. carinii isolates were 96% in Seoul Wistar rats, 93% in Seoul Sprague-Dawley rats, and 85% in Chinese Sprague-Dawley rats. The present finding confirmed that P. carinii from rats in Korea are grouped into two karyotype strains which are different from that of P. carinii from rats in China. The Chinese isolate shows a little different sequences of α-tubulin DNA.

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  • Intraspecific variability in several isolates of Philasterides dicentrarchi (syn. Miamiensis avidus), a scuticociliate parasite of farmed turbot
    Belén Budiño, Jesús Lamas, María P. Pata, Juan A. Arranz, Manuel L. Sanmartín, José Leiro
    Veterinary Parasitology.2011; 175(3-4): 260.     CrossRef
  • Analysis of Internal Transcribed Spacer 1 Sequences of Pneumocystis jiroveci from Clinical Specimens
    Jae-Seok Kim, Yong-Kyun Kim, Ji Young Park, Eun Kyung Mo, Han Sung Kim, Wonkeun Song, Hyoun Chan Cho, Kyu Man Lee
    Chonnam Medical Journal.2008; 44(2): 82.     CrossRef
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Persistent endemicity of Gymnophalloides seoi infection in a southwestern coastal village of Korea with special reference to its egg laying capacity in the human host
Jong-Yil Chai, Geun Chan Lee, Yun-Kyu Park, Eun-Taek Han, Min Seo, Jin Kim, Sang-Mee Guk, Eun-Hee Shin, Min-Ho Choi, Soon-Hyung Lee
Korean J Parasitol 2000;38(2):51-57.
Published online June 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.2.51

Follow-up studies have been conducted every three years on the endemicity of Gymnophalloides seoi infection in a small coastal village of Chollanam-do (Province), Korea, since it was first known as an endemic area in 1994. Special attention was given to its egg laying capacity in the human host. In fecal examinations, the overall helminth egg and/or cyst positive rate was 78.7% (74/94) in 1997 and 76.6% (82/107) in 2000. Among them G. seoi eggs showed the highest rate; 71.3% (67/94) in 1997 and 72.0% (77/107) in 2000. The average number of eggs per gram of feces (EPG) was 1,015 in 1997, while a reduced rate of 353 was observed in 2000. In 1997, total of 320,677 adult flukes of G. seoi (av. 10,344/person, 94-69,125 in range) were collected from the diarrheic stools of 31 treated patients. The EPG/worm obtained from 21 cases ranged from 0.04 to 0.77 (av. 0.23), suggesting density-dependent constraints on the worm fecundity. The relationship between the worm burden (X) and EPG/worm (Y) can be expressed as Y=0.42 ·e-1.2χ (r=0.49). The results showed that G. seoi infection is persistently endemic in this village.

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  • A potential zoonotic parasite, the digenean Gymnophalloides nacellae, on the Magellanic coast in the Southwestern Atlantic Ocean: its life cycle and geographical distribution
    Carmen Gilardoni, Gisele Di Giorgio, Estefanía Bagnato, Susana Pina, Pedro Rodrigues, Florencia Cremonte
    Polar Biology.2020; 43(6): 725.     CrossRef
  • Survey of Gymnophalloides seoi Metacercariae in Natural and Cultured Oysters from Several Western Coastal Areas, Korea
    Taehee Chang, Bong-Kwang Jung, Hyemi Song, Jaeeun Cho, Sooji Hong, Keon-Hoon Lee, Eui-Hyug Hoang, Jisu Kang, Jini Lim, Hana Lee, Jong-Yil Chai
    The Korean Journal of Parasitology.2019; 57(6): 705.     CrossRef
  • Current status of food-borne trematode infections
    R. Toledo, J. G. Esteban, B. Fried
    European Journal of Clinical Microbiology & Infectious Diseases.2012; 31(8): 1705.     CrossRef
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    Rafael Toledo, M. Dolores Bernal, Antonio Marcilla
    Journal of Proteomics.2011; 74(9): 1485.     CrossRef
  • Surveys of Gynaecotyla squatarolae and Microphallus koreana (Digenea: Microphallidae) Metacercariae in Two Species of Estuarine Crabs in Western Coastal Areas, Korea
    Hye Jung Lee, Jong-Yil Chai, Jin-Woo Lee, Ho Jin, Kwan-Hong Min, Yong-Jun Cho, Min Seo
    The Korean Journal of Parasitology.2010; 48(1): 81.     CrossRef
  • Intestinal Helminth Infections in Feral Cats and a Raccoon Dog on Aphaedo Island, Shinan-gun, with a Special Note on Gymnophalloides seoi Infection in Cats
    Eun-Hee Shin, Jae-Hwan Park, Sang-Mee Guk, Jae-Lip Kim, Jong-Yil Chai
    The Korean Journal of Parasitology.2009; 47(2): 189.     CrossRef
  • Foodborne Intestinal Flukes in Southeast Asia
    Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • A survey of Heterophyes nocens and Pygidiopsis summa metacercariae in mullets and gobies along the coastal areas of the Republic of Korea
    Sang-Mee Guk, Eun-Hee Shin, Jae-Lip Kim, Woon-Mok Sohn, Kwang-Sun Hong, Cheong-Ha Yoon, Soon-Hyung Lee, Han-Jong Rim, Jong-Yil Chai
    The Korean Journal of Parasitology.2007; 45(3): 205.     CrossRef
  • A new endemic focus of Heterophyes nocens and other heterophyid infections in a coastal area of Gangjin-gun, Jeollanam-do
    Jae-Hwan Park, Jae-Lip Kim, Eun-Hee Shin, Sang-Mee Guk, Yun-Kyu Park, Jong-Yil Chai
    The Korean Journal of Parasitology.2007; 45(1): 33.     CrossRef
  • A new endemic focus of Gymnophalloides seoi infection on Aphae Island, Shinan-gun, Jeollanam-do
    Jae-Hwan Park, Sang-Mee Guk, Eun-Hee Shin, Hyo-Jin Kim, Jae-Lip Kim, Min Seo, Yun-Kyu Park, Jong-Yil Chai
    The Korean Journal of Parasitology.2007; 45(1): 39.     CrossRef
  • Collection of Clonorchis sinensis adult worms from infected humans after praziquantel treatment
    Chenghua Shen, Jae-hwan Kim, Jeong-Keun Lee, Young Mee Bae, Min-Ho Choi, Jin-Kyoung Oh, Min Kyung Lim, Hai-Rim Shin, Sung-Tae Hong
    The Korean Journal of Parasitology.2007; 45(2): 149.     CrossRef
  • Prevalence of Gymnophalloides seoi infection in coastal villages of Haenam-gun and Yeongam-gun, Republic of Korea
    Sang-Mee Guk, Jae-Hwan Park, Eun-Hee Shin, Jae-Lip Kim, Aifen Lin, Jong-Yil Chai
    The Korean Journal of Parasitology.2006; 44(1): 1.     CrossRef
  • A case of colonic lymphoid tissue invasion by Gymnophalloides seoi in a Korean man
    Min Seo, Hokyung Chun, Geunghwan Ahn, Kee-Taek Jang, Sang-Mee Guk, Jong-Yil Chai
    The Korean Journal of Parasitology.2006; 44(1): 87.     CrossRef
  • Gymnophalloides seoi: a new human intestinal trematode
    Jong-Yil Chai, Min-Ho Choi, Jae-Ran Yu, Soon-Hyung Lee
    Trends in Parasitology.2003; 19(3): 109.     CrossRef
  • Food-borne intestinal trematode infections in the Republic of Korea
    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
  • Stictodora lari (Digenea: Heterophyidae): The Discovery of the First Human Infections
    J-Y. Chai, E-T. Han, Y-K. Park, S-M. Guk, J-H. Park, S-H. Lee
    Journal of Parasitology.2002; 88(3): 627.     CrossRef
  • A review of Gymnophalloides seoi (Digenea: Gymnophallidae) and human infections in the Republic of Korea
    Soon-Hyung Lee, Jong-Yil Chai
    The Korean Journal of Parasitology.2001; 39(2): 85.     CrossRef
  • A nationwide survey of the prevalence of human Gymnophalloides seoi infection on western and southern coastal islands in the Republic of Korea
    Jong-Yil Chai, Jae-Hwan Park, Eun-Taek Han, Eun-Hee Shin, Jae-Lip Kim, Kwang-Seon Hong, Han-Jong Rim, Soon-Hyung Lee
    The Korean Journal of Parasitology.2001; 39(1): 23.     CrossRef
  • 8,499 View
  • 80 Download
  • Crossref
Surface ultrastructure of Metagonimus takahashii metacercariae and adults
Jong-Yil Chai, Sang-Mee Guk, Eun-Taek Han, Min Seo, Eun-Hee Shin, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee
Korean J Parasitol 2000;38(1):9-15.
Published online March 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.1.9

A scanning electron microscopic study was performed on the surface ultrastructure of metacercariae and adults of Metagonimus takahashii. Metacercariae were collected from the scale of crucian carp (Carassius auratus), and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker had type I (numerous) and type II (seven in total) sensory papillae. Tegumental spines were dense and digitated into 5-7 points on the surface anterior to the ventral sucker, but became sparse and less digitated posteriorly toward the end of the body. In adults, seven type II sensory papillae were characteristically arranged around the lip of the oral sucker, and on the inner side of the lip four small and two large type I sensory papillae were symmetrically seen on each side (12 in total). Tegumental spines on anterior two-thirds of the body, were digitated with 9-12 tips ventrally and 8-13 tips dorsally. Sperms entering into the Laurer's canal were observed. The results show that the surface ultrastructure of M. takahashii is generally similar to those of M. yokogawai and M. miyatai except for the digitation of tegumental spines.

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    Jean‐José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
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    Journal of Parasitology.2011; 97(6): 1049.     CrossRef
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    Jean-José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
    Journal of Parasitology.2010; 96(6): 1102.     CrossRef
  • The life cycle of Ascocotyle (Phagicola) longa (Digenea: Heterophyidae), a causative agent of fish-borne trematodosis
    Susana Balmant Emerique Simões, Helene Santos Barbosa, Cláudia Portes Santos
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  • TAXONOMIC STATUS, REDESCRIPTION, AND SURFACE ULTRASTRUCTURE OF ASCOCOTYLE (PHAGICOLA) PINDORAMENSIS N. COMB. (DIGENEA: HETEROPHYIDAE)
    Susana Balmant Emerique Simões, Tomáš Scholz, Helene Santos Barbosa, Cláudia Portes Santos
    Journal of Parasitology.2006; 92(3): 501.     CrossRef
  • Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
    Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
    The Korean Journal of Parasitology.2004; 42(3): 129.     CrossRef
  • Surface ultrastructure of the adult stage of Acanthotrema felis (Trematoda: Heterophyidae)
    Woon-Mok Sohn, Min Seo, Jong-Yil Chai
    The Korean Journal of Parasitology.2003; 41(2): 107.     CrossRef
  • Tegumental ultrastructure of Echinoparyphium recurvatum according to developmental stages
    Woon-Mok Sohn, Ho-Choon Woo, Sung-Jong Hong
    The Korean Journal of Parasitology.2002; 40(2): 67.     CrossRef
  • Surface ultrastructure of Pygidiopsis summa (Digenea: Heterophyidae) adult flukes
    Jong-Yil Chai, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee
    The Korean Journal of Parasitology.2002; 40(3): 107.     CrossRef
  • Brachylaima cribbi (Digenea: Brachylaimidae): scanning electron microscopical observations of the life-cycle stages
    A.R. Butcher, J.K. Brealey, D.I. Grove, R.B. Dymock
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Brief Communication

High endemicity of Metagonimus yokogawai infection among residents of Samchok-shi, Kangwon-do
Jong-Yil Chai, Eun-Taek Han, Yun-Kyu Park, Sang-Mee Guk, Jae-Lip Kim, Soon-Hyung Lee
Korean J Parasitol 2000;38(1):33-36.
Published online March 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.1.33

A small-scale epidemiological survey was undertaken during 1997-1998 on the residents along the Osib-chon (Stream), Samchok-shi (City), Kangwon-do (Province), to evaluate the status of Metagonimus yokogawai infection. A total of 165 fecal samples was collected and examined by cellophane thick smear and formalin-ether sedimentation techniques. The egg positive rate of M. yokogawai was 29.7%, showing a remarkable difference between males (46.6%) and females (16.3%). To obtain the adult flukes of M. yokogawai, 11 egg positive persons were treated with praziquantel and purged with magnesium sulfate. A total of 242,119 adult flukes (average 22,010 per person, 367-119,650 in range) was collected from diarrheic stools, all of which were identified as M. yokogawai. The results show that M. yokogawai is still highly endemic in this area.

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  • Re‐evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 2
    Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Lieve Herman, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panag
    EFSA Journal.2024;[Epub]     CrossRef
  • Prevalence and spatial distribution of heterophyidiasis in Southern Philippines
    Kharleezelle J. Moendeg, Lydia R. Leonardo, Trina Galido Isorena, Francine Claire Alexandra S. Hilotina, Imelda S. Pates, Nancy O. Cacayorin
    Acta Tropica.2021; 220: 105940.     CrossRef
  • Foodborne intestinal flukes: A brief review of epidemiology and geographical distribution
    Jong-Yil Chai, Bong-Kwang Jung
    Acta Tropica.2020; 201: 105210.     CrossRef
  • Fish substitutions which may increase human health risks from zoonotic seafood borne parasites: A review
    Michelle Williams, Marta Hernandez-Jover, Shokoofeh Shamsi
    Food Control.2020; 118: 107429.     CrossRef
  • Infection Status with Metagonimus spp. Metacercariae in Fishes from Seomjin-gang and Tamjin-gang in Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Cheon-Hyeon Kim, Ki-Bok Yoon
    The Korean Journal of Parasitology.2018; 56(4): 351.     CrossRef
  • Metagonimus suifunensis sp. n. (Trematoda: Heterophyidae) from the Russian Southern Far East: Morphology, life cycle, and molecular data
    P.G. Shumenko, Y.V. Tatonova, V.V. Besprozvannykh
    Parasitology International.2017; 66(1): 982.     CrossRef
  • Fishborne zoonotic heterophyid infections: An update
    Jong-Yil Chai, Bong-Kwang Jung
    Food and Waterborne Parasitology.2017; 8-9: 33.     CrossRef
  • A molecular phylogeny of Asian species of the genus Metagonimus (Digenea)—small intestinal flukes—based on representative Japanese populations
    Siritavee Pornruseetairatn, Hideto Kino, Takeshi Shimazu, Yukifumi Nawa, Tomáš Scholz, Jiraporn Ruangsittichai, Naowarat Tanomsing Saralamba, Urusa Thaenkham
    Parasitology Research.2016; 115(3): 1123.     CrossRef
  • Heterophyid trematodes recovered from people residing along the Boseong River, South Korea
    Jong-Yil Chai, Bong-Kwang Jung, Deok-Gyu Kim, Jae-Lip Kim, Hyemi Lim, Eun-Hee Shin, Keon Hoon Lee, Mok Ryeon Kim, Sun Jin Han, Jae Hyun Yeom, Sun Mi Park, Jae-Sun Hwang
    Acta Tropica.2015; 148: 142.     CrossRef
  • Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes
    Eun-Taek Han, Hyun-Jong Yang, Young-Jin Park, Jeong-Hyun Park, Jong-Yil Chai
    The Korean Journal of Parasitology.2014; 52(2): 201.     CrossRef
  • Prevalence ofMetagonimusMetacercariae in Sweetfish,Plecoglossus altivelis, from Eastern and Southern Coastal Areas in Korea
    Shin-Hyeong Cho, Tong-Soo Kim, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2011; 49(2): 161.     CrossRef
  • Fish-borne Parasitic Diseases
    Jong-Yil Chai
    Hanyang Medical Reviews.2010; 30(3): 223.     CrossRef
  • Foodborne Intestinal Flukes in Southeast Asia
    Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • A Nationwide Survey on the Prevalence of Intestinal Parasitic Infections in the Republic of Korea, 2004
    Tong-Soo Kim, Shin-Hyeong Cho, Sun Huh, Yoon Kong, Woon-Mok Sohn, Seung-Sik Hwang, Jong-Yil Chai, Soon-Hyung Lee, Yun-Kyu Park, Dae-Kyu Oh, Jong-Koo Lee
    The Korean Journal of Parasitology.2009; 47(1): 37.     CrossRef
  • Review of Zoonotic Parasites in Medical and Veterinary Fields in the Republic of Korea
    Heejeong Youn
    The Korean Journal of Parasitology.2009; 47(Suppl): S133.     CrossRef
  • Decrease of Metagonimus yokogawai Endemicity along the Tamjin River Basin
    Jin-Ju Lee, Hyo-Jin Kim, Min-Jae Kim, Jo Woon Yi Lee, Bong-Kwang Jung, Ji-Youn Lee, Eun-Hee Shin, Jae-Lip Kim, Jong-Yil Chai
    The Korean Journal of Parasitology.2008; 46(4): 289.     CrossRef
  • SUSCEPTIBILITY OF INBRED MOUSE STRAINS TO INFECTION WITH THREE SPECIES OF METAGONIMUS PREVALENT IN THE REPUBLIC OF KOREA
    Sang-Mee Guk, Jin-Young Park, Min Seo, Eun-Taek Han, Jae-Lip Kim, Jong-Yil Chai
    Journal of Parasitology.2005; 91(1): 12.     CrossRef
  • Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
    Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
    The Korean Journal of Parasitology.2004; 42(3): 129.     CrossRef
  • Food-borne Parasitic Diseases
    Jong-Yil Chai
    Journal of the Korean Medical Association.2004; 47(6): 496.     CrossRef
  • Food-borne intestinal trematode infections in the Republic of Korea
    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
  • Epidemiological study of clonorchiasis and metagonimiasis along the Geum-gang (River) in Okcheon-gun (County), Korea
    Gye-Sung Lee, In-Sung Cho, Young-Ha Lee, Hyung-Jun Noh, Dae-Whan Shin, Sok-Goo Lee, Tae-Yong Lee
    The Korean Journal of Parasitology.2002; 40(1): 9.     CrossRef
  • Molecular Differentiation of Three Species ofMetagonimusby Simple Sequence Repeat Anchored Polymerase Chain Reaction (SSR-PCR) Amplification
    H-J. Yang, S-M. Guk, E-T. Han, J-Y. Chai
    Journal of Parasitology.2000; 86(5): 1170.     CrossRef
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Original Articles

Karyotypes of Pneumocystis carinii derived from several mammals
Sang Rock Cho, Yun-Gyu Park, Hyung Nam Moon, Soon-Hyung Lee, Sung-Tae Hong
Korean J Parasitol 1999;37(4):271-275.
Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.271

Pneumocystis carinii is the most important opportunistic pathogen of humans in the world. Pneumocystis carinii is experimentally detected in the lungs of rats, mice, rabbits, and monkeys, however, the organisms from different mammals are identical in microscopic morphology. The present study tried to find out more mammalian hosts of P. carinii and also to differentiate the organisms from different mammals by karyotyping. Rats, mice, hamsters, rabbits, cats, and dogs were successfully infected by P. carinii, but guinea pigs and pigs were not. Karyotype of P. carinii from rabbits showed similar size range of chromosomes with that of the prototype, but in different pattern. The patterns from cats and dogs were also different from that of rats. The present study confirms that cats and dogs are infected by P. carinii and at least total three karyotype strains of P. carinii are proven in Korea.

Citations

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  • Axenic Long-Term Cultivation of Pneumocystis jirovecii
    Diana Riebold, Marie Mahnkopf, Kristina Wicht, Cristina Zubiria-Barrera, Jan Heise, Marcus Frank, Daniel Misch, Torsten Bauer, Hartmut Stocker, Hortense Slevogt
    Journal of Fungi.2023; 9(9): 903.     CrossRef
  • Respiratory Diseases in Guinea Pigs, Chinchillas and Degus
    María Ardiaca García, Andrés Montesinos Barceló, Cristina Bonvehí Nadeu, Vladimír Jekl
    Veterinary Clinics of North America: Exotic Animal Practice.2021; 24(2): 419.     CrossRef
  • Molecular detection ofPneumocystisin the lungs of cats
    Patrizia Danesi, Michela Corrò, Christian Falcaro, Antonio Carminato, Tommaso Furlanello, Monia Cocchi, Mark B Krockenberger, Wieland Meyer, Gioia Capelli, Richard Malik
    Medical Mycology.2018;[Epub]     CrossRef
  • Pneumocystis: from a doubtful unique entity to a group of highly diversified fungal species
    Magali Chabé, Cécile-Marie Aliouat-Denis, Laurence Delhaes, El Moukhtar Aliouat, Eric Viscogliosi, Eduardo Dei-Cas
    FEMS Yeast Research.2011; 11(1): 2.     CrossRef
  • Analysis of Internal Transcribed Spacer 1 Sequences of Pneumocystis jiroveci from Clinical Specimens
    Jae-Seok Kim, Yong-Kyun Kim, Ji Young Park, Eun Kyung Mo, Han Sung Kim, Wonkeun Song, Hyoun Chan Cho, Kyu Man Lee
    Chonnam Medical Journal.2008; 44(2): 82.     CrossRef
  • Pneumocystis species, co-evolution and pathogenic power
    Cécile-Marie Aliouat-Denis, Magali Chabé, Christine Demanche, El Moukhtar Aliouat, Eric Viscogliosi, Jacques Guillot, Laurence Delhaes, Eduardo Dei-Cas
    Infection, Genetics and Evolution.2008; 8(5): 708.     CrossRef
  • Pneumocystis
    James R. Stringer
    International Journal of Medical Microbiology.2002; 292(5-6): 391.     CrossRef
  • Genetic heterogeneity of Pneumocystis carinii from rats of several regions and strains
    Byung-Suk Chung, Yun-Kyu Pars, Sun Huh, Jae-Ran Yu, Jin Kim, Xiaohua Shi, Sang Rock Cho, Soon-Hyung Lee, Sung-Tae Hong
    The Korean Journal of Parasitology.2000; 38(3): 151.     CrossRef
  • 8,836 View
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Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis
Sung-Tae Hong, Mejeong Lee, Nak-Jin Sung, Sang Rock Cho, Jong-Yil Chai, Soon-Hyung Lee
Korean J Parasitol 1999;37(4):243-248.
Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.243

The present study analyzed serum IgG subclass antibody reaction to major antigenic bands of Clonorchis sinensis to investigate improvement of its serodiagnosis. Of the four subclass antibodies, IgG1 and IgG2 antibodies were produced but not specific, IgG3 antibody was least produced, and IgG4 antibody was prominent and specific. The serum IgG antibody reaction to any of 43-50, 34-37, 26-28, and 8 kDa bands was found in 65.5% of 168 egg positive cases while IgG4 antibody reaction was found in 22.0% of them. The positive rates of IgG and IgG4 antibodies were directly correlated with the intensity of infection. All of the sera from heavily infected cases over EPG 5,000 showed positive reaction for specific IgG and IgG4 antibodies. The specific serum IgG4 antibody disappeared within 6 months after treatment. The bands of 35 kDa and 67 kDa cross-reacted with IgG antibodies but not with IgG4 antibodies in sera of other trematode infections. The present findings suggest that serum IgG4 antibody reaction to 8 kDa band is specific but not sensitive. Any method to increase its sensitivity is required for improved serodiagnosis.

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  • Initial misdiagnosis to a revised diagnosis of pulmonary paragonimiasis with elevated serum immunoglobulin G4 concentrations: a case report
    Pengyan Yin, Miaohui Duan, Lingyao Deng, Tianli Li, Xuan Huang, Raohong Fang, Hailong Li, Binliang Bai
    Journal of International Medical Research.2025;[Epub]     CrossRef
  • An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection
    Lakkhana Sadaow, Rutchanee Rodpai, Penchom Janwan, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Hiroshi Yamasaki, Wannaporn Ittiprasert, Victoria H. Mann, Paul J. Brindley, Wanchai Maleewong, Pewpan M. Intapan
    Tropical Medicine and Infectious Disease.2022; 7(10): 308.     CrossRef
  • High prevalence of opisthorchiasis in rural populations from Khammouane Province, central Lao PDR: serological screening using total IgG- and IgG4-based ELISA
    Weeraya Phupiewkham, Rutchanee Rodpai, Somchith Inthavongsack, Sakhone Laymanivong, Tongjit Thanchomnang, Lakkhana Sadaow, Patcharaporn Boonroumkaew, Oranuch Sanpool, Penchom Janwan, Pewpan M Intapan, Wanchai Maleewong
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2021; 115(12): 1403.     CrossRef
  • Comparative assessment of immunochromatographic test kits using somatic antigens from adult Opisthorchis viverrini and IgG and IgG4 conjugates for serodiagnosis of human opisthorchiasis
    Weeraya Phupiewkham, Lakkhana Sadaow, Oranuch Sanpool, Rutchanee Rodpai, Hiroshi Yamasaki, Wannaporn Ittiprasert, Victoria H. Mann, Paul J. Brindley, Wanchai Maleewong, Pewpan M. Intapan
    Parasitology Research.2021; 120(8): 2839.     CrossRef
  • Development of an Immunochromatographic Point-of-Care Test for Serodiagnosis of Opisthorchiasis and Clonorchiasis
    Lakkhana Sadaow, Oranuch Sanpool, Rutchanee Rodpai, Hiroshi Yamasaki, Wannaporn Ittiprasert, Victoria H. Mann, Paul J. Brindley, Wanchai Maleewong, Pewpan M. Intapan
    The American Journal of Tropical Medicine and Hygiene.2019; 101(5): 1156.     CrossRef
  • The serological diagnosis of human clonorchiasis by time-resolved fluoroimmunoassay based on GST2-specific IgG4 detection
    Jin Xu, Lexun Wang, Chuanhuan Deng, Xuchu Hu, Ran Li, Tingjin Chen, Dan Ning, Yingsong Wu, Yan Huang, Xuerong Li, Zhongdao Wu, Xinbing Yu, Yonglong Li
    Parasitology Research.2014; 113(1): 149.     CrossRef
  • 41.5-kDa Cathepsin L protease from Clonorchis sinensis: expression, characterization, and serological reactivity of one excretory–secretory antigen
    Yanwen Li, Yan Huang, Xuchu Hu, Xiaoquan Liu, Changling Ma, Junhong Zhao, Zhongdao Wu, Jin Xu, Xinbing Yu
    Parasitology Research.2012; 111(2): 673.     CrossRef
  • Evaluation and Characterization of Fasciola hepatica Tegument Protein Extract for Serodiagnosis of Human Fascioliasis
    Adelaida Morales, Ana M. Espino
    Clinical and Vaccine Immunology.2012; 19(11): 1870.     CrossRef
  • Serodiagnostic applicability of recombinant antigens of Clonorchis sinensis expressed by wheat germ cell-free protein synthesis system
    Chenghua Shen, Jong-Ae Lee, Sonia Refaat Ahmed Allam, Young Mee Bae, Eun-Taek Han, Satoru Takeo, Takafumi Tsuboi, Sung-Tae Hong, Min-Ho Choi
    Diagnostic Microbiology and Infectious Disease.2009; 64(3): 334.     CrossRef
  • The identification of a Clonorchis sinensis gene encoding an antigenic egg protein
    Mejeong Lee, Young-Bae Chung, Suk-Keun Lee, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
    Parasitology Research.2005; 95(3): 224.     CrossRef
  • Evaluation of Immunoglobulin G Subclass Antibodies against RecombinantFasciola giganticaCathepsin L1 in an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Fasciolosis
    Chaisiri Wongkham, Chairat Tantrawatpan, Pewpan M. Intapan, Wanchai Maleewong, Sopit Wongkham, Kunio Nakashima
    Clinical and Vaccine Immunology.2005; 12(10): 1152.     CrossRef
  • Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA
    Min-Ho Choi, Il Chan Park, Shunyu Li, Sung-Tae Hong
    The Korean Journal of Parasitology.2003; 41(1): 35.     CrossRef
  • Immunology and molecular biology of Opisthorchis viverrini infection
    S. Wongratanacheewin, R.W. Sermswan, S. Sirisinha
    Acta Tropica.2003; 88(3): 195.     CrossRef
  • Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda)
    Min-Ho Choi, Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong
    The Korean Journal of Parasitology.2003; 41(3): 155.     CrossRef
  • Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis
    Young-Bae Chung, Mejeong Lee, Hyun-Jong Yang, Byung-Suk Chung, Shun-Yu Lee, Min-Ho Choi, Sung-Tae Hong
    The Korean Journal of Parasitology.2002; 40(2): 83.     CrossRef
  • Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection
    Sung-Jong Hong, Tae Yun Kim, Kye-Yong Song, Woon-Mok Sohn, Shin-Yong Kang
    The Korean Journal of Parasitology.2001; 39(4): 307.     CrossRef
  • Cystatin Capture Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Clonorchiasis and Profile of Captured Antigenic Protein ofClonorchis sinensis
    Tae Yun Kim, Shin-Yong Kang, Sun Hyo Park, Kom Sukontason, Kabkaew Sukontason, Sung-Jong Hong
    Clinical Diagnostic Laboratory Immunology.2001; 8(6): 1076.     CrossRef
  • Partial characterization of a 17 kDa protein of Clonorchis sinensis
    Young-Bae Chung, Byung-Suk Chung, Min-Ho Choi, Jong-Yil Chai, Sung-Tae Hong
    The Korean Journal of Parasitology.2000; 38(2): 95.     CrossRef
  • 8,983 View
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Case Report

An incidental case of human Heterophyes nocens infection diagnosed by sectional morphology in a biopsy specimen of the small intestine
Yong-Suk Ryang, Chi-Young Lee, Kyu-Jae Lee, Soon-Hyung Lee, Jong-Yil Chai
Korean J Parasitol 1999;37(3):189-194.
Published online September 30, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.3.189

A case of human infection with Heterophyes nocens (Heterophyidae) was incidentally found in a biopsy specimen of the Meckel's diverticulum at the upper part of the small intestine. The patient was a 58-year-old man living in a rural area of Talsong-gun, Kyongsangbuk-do. He had gastrointestinal symptoms such as epigastric pain, indigestion, and abdominal discomfort for 3 months, and severe diarrhea, abdominal pain, and vomiting for about 1 month before hospitalization. Endoscopy of the upper part of the small intestine revealed a Meckel's diverticulum, and it was excised and histopathologically examined. Three adult flukes were incidentally found sectioned in the mucosa, and they were identified as H. nocens. The patient had a history of eating raw mullets at a fish market in Pusan 6 months ago, and the mullets were presumed to be the source of infection. This case brings a considerable interest in that specific diagnosis of heterophyid infections could be done by sectional morphology of the worms.

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  • Prevalence and spatial distribution of heterophyidiasis in Southern Philippines
    Kharleezelle J. Moendeg, Lydia R. Leonardo, Trina Galido Isorena, Francine Claire Alexandra S. Hilotina, Imelda S. Pates, Nancy O. Cacayorin
    Acta Tropica.2021; 220: 105940.     CrossRef
  • Fishborne zoonotic heterophyid infections: An update
    Jong-Yil Chai, Bong-Kwang Jung
    Food and Waterborne Parasitology.2017; 8-9: 33.     CrossRef
  • Co-infection with Opisthorchis viverrini and Haplorchis taichui detected by human fecal examination in Chomtong district, Chiang Mai Province, Thailand
    Chalobol Wongsawad, Anawat Phalee, Waraporn Noikong, Suksan Chuboon, Choosak Nithikathkul
    Parasitology International.2012; 61(1): 56.     CrossRef
  • Haplorchis taichuias a Possible Etiologic Agent of Irritable Bowel Syndrome-Like Symptoms
    Dorn Watthanakulpanich, Jitra Waikagul, Wanna Maipanich, Supaporn Nuamtanong, Surapol Sanguankiat, Somchit Pubampen, Rangson Praevanit, Srisuchat Mongkhonmu, Yukifumi Nawa
    The Korean Journal of Parasitology.2010; 48(3): 225.     CrossRef
  • 7,731 View
  • 79 Download
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Original Articles
A 54 kDa cysteine protease purified from the crude extract of Neodiplostomum seoulense adult worms
Min-Ho Choi, Seong-Choon Choe, Soon-Hyung Lee
Korean J Parasitol 1999;37(1):39-46.
Published online March 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.1.39

As a preliminary study for the explanation of pathobiology of Neodiplostomum seoulense infection, a 54 kDa protease was purified from the crude extract of adult worms by sequential chromatographic methods. The crude extract was subjected to DEAE-Sepharose Fast Flow column, and protein was eluted using 25 mM Tris-HCl (pH 7.4) containing 0.05, 0.1, 0.2 and 0.4 M NaCl in stepwise elution. The 0.2 M NaCl fraction was further purified by Q-Sepharose chromatography and protein was eluted using 20 mM sodium acetate (pH 6.4) containing 0.05, 0.1, 0.2 and 0.3 M NaCl, respectively. The 0.1M NaCl fraction showed a single protein band on SDS-PAGE carried out on a 7.5-15% gradient gel. The proteolytic activities of the purified enzyme were specifically inhibited by L-trans-epoxy-succinylleucylamide (4-guanidino) butane (E-64) and iodoacetic acid. The enzyme, cysteine protease, showed the maximum proteolytic activity at pH 6.0 in 0.1 M buffer, and degraded extracellular matrix proteins such as collagen and fibronectin with different activities. It is suggested that the cysteine protease may play a role in the nutrient uptake of N. seoulense from the host intestine.

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    Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • Antigenic Properties of Cystatin-binding Cysteine Proteinases from Neodiplostomum seoulense
    Tae-Yun Kim, Kye-Young Han, Eun-Hee Shin, Jong-Yil Chai
    Journal of Parasitology.2008; 94(3): 654.     CrossRef
  • Antibody Responses in Sera of Different Mouse Strains Experimentally Infected with Neodiplostomum seoulense
    Eun-Taek Han, Jun-Hu Chen, Jong-Yil Chai
    The Korean Journal of Parasitology.2008; 46(4): 279.     CrossRef
  • Antigenic Properties of Cystatin-binding Cysteine Proteinases from Neodiplostomum seoulense
    Tae-Yun Kim, Kye-Young Han, Eun-Hee Shin, Jong-Yil Chai
    Journal of Parasitology.2008; 94(3): 654.     CrossRef
  • Food-borne intestinal trematode infections in the Republic of Korea
    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
  • Cystatin Capture Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Clonorchiasis and Profile of Captured Antigenic Protein ofClonorchis sinensis
    Tae Yun Kim, Shin-Yong Kang, Sun Hyo Park, Kom Sukontason, Kabkaew Sukontason, Sung-Jong Hong
    Clinical Diagnostic Laboratory Immunology.2001; 8(6): 1076.     CrossRef
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Growth and development of Gymnophalloides seoi in immunocompetent and immunosuppressed C3H/HeN mice
Jong-Yil Chai, Won-Jae Chung, Jina Kook, Min Seo, Yun-Kyu Park, Sang-Mee Guk, Min-Ho Choi, Soon-Hyung Lee
Korean J Parasitol 1999;37(1):21-26.
Published online March 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.1.21

The growth and development of Gymnophalloides seoi were studied in C3H/HeN mice and effects of immunosuppression of the host on the worm development were observed. Two hundred metacercariae of G. seoi were orally administered to each mouse, and worms were recovered on days 1, 3, 5, 7, 14 and 21 post-infection (PI). The worm recovery rate was significantly higher in immunosuppressed (ImSP) mice than in immunocompetent (ImCT) mice except on days 1 and 3 PI. The worms attained sexual maturity by day 3 PI with eggs in the uterus, and worm dimensions and the number of uterine eggs continuously increased until day 14 PI in ImSP mice. Worms recovered from ImSP mice were significantly larger in size than those from ImCT mice on days 1 and 3 PI, and the number of uterine eggs was significantly larger in ImSP mice on days 5 and 7 PI. Genital organs such as the ovary, testes, and vitellaria, that were already developed in the metacercarial stage, grew a little in size until day 14 PI. The results show that the C3H/HeN mouse is, though not excellent, a suitable laboratory host for G. seoi.

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  • Increased Intestinal Epithelial Cell Turnover and Intestinal Motility in Gymnophalloides seoi-Infected C57BL/6 Mice
    Sang Hyub Lee, Bong-Kwang Jung, Jae-Hwan Park, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2014; 52(3): 273.     CrossRef
  • Foodborne Intestinal Flukes in Southeast Asia
    Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • Gymnophalloides seoi: a new human intestinal trematode
    Jong-Yil Chai, Min-Ho Choi, Jae-Ran Yu, Soon-Hyung Lee
    Trends in Parasitology.2003; 19(3): 109.     CrossRef
  • Food-borne intestinal trematode infections in the Republic of Korea
    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
  • A review of Gymnophalloides seoi (Digenea: Gymnophallidae) and human infections in the Republic of Korea
    Soon-Hyung Lee, Jong-Yil Chai
    The Korean Journal of Parasitology.2001; 39(2): 85.     CrossRef
  • Intestinal histopathology and in situ postures of Gymnophalloides seoi in experimentally infected mice
    Jong-Yil Chai, Hong-Soon Lee, Sung-Jong Hong, Jae Hyung Yoo, Sang-Mee Guk, Min Seo, Min-Ho Choi, Soon-Hyung Lee
    The Korean Journal of Parasitology.2001; 39(1): 31.     CrossRef
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Control of clonorchiasis by repeated praziquantel treatment and low diagnostic efficacy of sonography
Sung-Tae Hong, Kisung Yoon, Mejeong Lee, Min Seo, Min-Ho Choi, Jung Suk Sim, Byung Ihn Choi, Chong Ku Yun, Soon-Hyung Lee
Korean J Parasitol 1998;36(4):249-254.
Published online December 20, 1998
DOI: https://doi.org/10.3347/kjp.1998.36.4.249

In Korea, Clonorchis sinensis infection is still highly prevalent because case detection in the field is difficult and the detected cases used to be incompletely cured due to treatment failure. The present study tried to control clonorchiasis in an endemic village by repeated treatments with praziquantel every 6 months and to evaluate sonography as a diagnostic measure. By stool examinations, the egg positive rate in the endemic village was 22.7%, but it decreased to 19.6% at 6 months, 15.1% at 12 months. 12.2% at 18 months, 6.3% at 24 months, 11.4% at 30 months, and 6.3% at 42 months after the beginning of repeated praziquantel administration. The sonography showed 61 (49.6%) positive cases of 123 screened residents: among egg-positives the sonography positive rate was 52.2% and among egg-negatives it was still 49%. The rate among cured cases was 64.3% after 6 months, 50.0% after 12 months, 50.0% after 18 months, and 66.7% after 24 months. In a non-endemic village, 64 residents were found egg-negative by fecal examination, but 20 (31.3%) of them were positive by sonography. The present findings indicate that control of clonorchiasis in an endemic village by repeated praziquantel treatment for 42 months is still insufficient and sonography is of little value for diagnosis of clonorchiasis.

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    Liina Kinkar, Pasi K. Korhonen, Urmas Saarma, Tao Wang, Xing‐Quan Zhu, Ivon Harliwong, Bicheng Yang, J. Lynn Fink, Daxi Wang, Bill C. H. Chang, Galina N. Chelomina, Anson V. Koehler, Neil D. Young, Robin B. Gasser
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    Men-Bao Qian, Chang-Hai Zhou, Hui-Hui Zhu, Ying-Dan Chen, Xiao-Nong Zhou
    Infectious Diseases of Poverty.2021;[Epub]     CrossRef
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    J.-G. Kim, C.-S. Ahn, B. Sripa, K.S. Eom, I. Kang, W.-M. Sohn, Y. Nawa, Y. Kong
    Clinical Microbiology and Infection.2019; 25(1): 109.e1.     CrossRef
  • A Clonorchiasis Case of a Leopard Cat, Prionailurus bengalensis euptilurus, Diagnosed by Ultrasonography and Egg Detection in Republic of Korea
    Seongjun Choe, Dong-Hyuk Jeong, Jeong-Jin Yang, Jeongho Kim, Ki-Jeong Na, Dongmin Lee, Hansol Park, Hyeong-Kyu Jeon, Keeseon S. Eom
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    The American Journal of Tropical Medicine and Hygiene.2017; 97(3): 888.     CrossRef
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    Ze-Li Tang, Yan Huang, Xin-Bing Yu
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    Min-Ho Choi, Sue K. Park, Zhimin Li, Zhuo Ji, Gui Yu, Zheng Feng, Longqi Xu, Seung-Yull Cho, Han-Jong Rim, Soon-Hyung Lee, Sung-Tae Hong, Banchob Sripa
    PLoS Neglected Tropical Diseases.2010; 4(2): e601.     CrossRef
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    Dongil Choi, Yong Hwan Jeon, Geun-Chan Lee, Min-Ho Choi, Sung-Tae Hong
    The Korean Journal of Parasitology.2009; 47(1): 19.     CrossRef
  • Collection of Clonorchis sinensis adult worms from infected humans after praziquantel treatment
    Chenghua Shen, Jae-hwan Kim, Jeong-Keun Lee, Young Mee Bae, Min-Ho Choi, Jin-Kyoung Oh, Min Kyung Lim, Hai-Rim Shin, Sung-Tae Hong
    The Korean Journal of Parasitology.2007; 45(2): 149.     CrossRef
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    Dongil Choi, Sung-Tae Hong
    The Korean Journal of Parasitology.2007; 45(2): 77.     CrossRef
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    J.‐S. Lee, I. S. Kim, W.‐M. Sohn, J. Lee, T.‐S. Yong
    Scandinavian Journal of Immunology.2006; 63(3): 169.     CrossRef
  • A successful experience of soil-transmitted helminth control in the Republic of Korea
    Sung-Tae Hong, Jong-Yil Chai, Min-Ho Choi, Sun Huh, Han-Jong Rim, Soon-Hyung Lee
    The Korean Journal of Parasitology.2006; 44(3): 177.     CrossRef
  • Therapeutic evaluation of sustained-releasing praziquantel (SRP) for clonorchiasis: Phase 1 and 2 clinical studies
    Min-Ho Choi, Byung-Chan Chang, Seung-Jin Lee, In-Jin Jang, Sang-Goo Shin, Weon-Gyu Kho, Jin-Ho Chun, Sung-Tae Hong
    The Korean Journal of Parasitology.2006; 44(4): 361.     CrossRef
  • Bile duct changes in rats reinfected with Clonorchis sinensis
    Dongil Choi, Sung-Tae Hong, Shunyu Li, Byung-Suk Chung, Jae Hoon Lim, Soon-Hyung Lee
    The Korean Journal of Parasitology.2004; 42(1): 7.     CrossRef
  • Sonographic findings of active Clonorchis sinensis infection
    Dongil Choi, Sung‐Tae Hong, Jae Hoon Lim, Seung‐Yull Cho, Han‐Jong Rim, Zhuo Ji, Ren Yuan, Shuyu Wang
    Journal of Clinical Ultrasound.2004; 32(1): 17.     CrossRef
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    Byung Ihn Choi, Joon Koo Han, Sung Tae Hong, Kyoung Ho Lee
    Clinical Microbiology Reviews.2004; 17(3): 540.     CrossRef
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    Sung-Tae Hong, Min-Ho Choi, Chung-Hyun Kim, Byung-Suk Chung, Zhuo Ji
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    Sandie King, Tomáš Scholz
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    Sung-Tae Hong, Han-Jong Rim, Duk-Young Min, Xueming Li, Jingxian Xu, Zheng Feng, Soon-Hyung Lee
    The Korean Journal of Parasitology.2001; 39(4): 285.     CrossRef
  • Long-lasting sonographic and histopathological findings in cured clonorchiasis of rabbits
    Dongil Choi, Jae Hoon Lim, Seung Kwon Kim, Eung Yeop Kim, Mejeong Lee, Sung-Tae Hong
    The Korean Journal of Parasitology.1999; 37(2): 77.     CrossRef
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Surface ultrastructure of Metagonimus miyatai metacercariae and adults
Jong-Yil Chai, Young-Je Kang, Sung-Yil Choi, Sang-Mee Guk, Jae-Ran Yu, Soon-Hyung Lee
Korean J Parasitol 1998;36(4):217-225.
Published online December 20, 1998
DOI: https://doi.org/10.3347/kjp.1998.36.4.217

A scanning electron microscopic study was performed to observe surface ultrastructures of excysted metacercariae and adults of Metagonimus miyatai. Metacercariae were collected from the scale of the pale chub (Zacco platypus), and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker was devoid of tegumental spines and had type I and type II sensory papillae. Anteriorly to the ventral sucker, spines were dense and digitated into 5-7 points, whereas near the posterior end of the body spines were sparse and digitated into 2-3 points. In one-week adults, 7 type II sensory papillae were arranged around the lip of the oral sucker, and at inner side of the lip one pair of small and two pairs of large type 1 sensory papillae were seen on each side. The distribution of tegumental spines was similar to that of metacercariae, but they were more differentiated with 9-11 pointed tips. In two- to four-week old adults, the surface ultrastructure was nearly the same as in one-week old adults, however, sperms were frequently seen entering into the Laurer's canal. Conclusively, the surface ultrastructure of M. miyatai was generally similar to that of M. yokogawai, however, differentiation of tegumental spines and distribution of sensory papillae around the oral sucker were different between the two species, which may be of taxonomic significance.

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  • Surface ultrastructure of the adult and juvenile stages of the trematode Astiotrema impletum (Looss, 1899) Looss 1900 (incertae sedis) from the Nile puffer, Tetraodon lineatus Linnaeus, 1758
    S. G. Abd El-Kareem, M. H. Ibraheem
    Helminthologia.2021; 58(2): 188.     CrossRef
  • Digenean Holostephanus (Trematoda: Digenea: Cyathocotylidae) metacercariae in common carp (Cyprinus carpio Linnaeus, 1758) muscle: zoonotic potential and sensitivity to physico-chemical treatments
    D. Sándor, M. Gyöngy, K. Nyeste, I. Czeglédi, C. Székely, K. Buchmann, G. Cech
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Metagonimus yokogawai Ancient DNA Recovered from 16th- to 17th-Century Korean Mummy Feces of the Joseon Dynasty
    Jong Ha Hong, Min Seo, Chang Seok Oh, Jong-Yil Chai, Dong Hoon Shin
    Journal of Parasitology.2020;[Epub]     CrossRef
  • Topography and ultrastructure of the tegument of Deropristis inflata Molin, 1859 (Digenea: Deropristidae), a parasite of the European eel Anguilla anguilla (Osteichthyes: Anguillidae)
    Jean-José Filippi, Yann Quilichini, Bernard Marchand
    Parasitology Research.2013; 112(2): 517.     CrossRef
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    Jean‐José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
    Journal of Morphology.2012; 273(4): 361.     CrossRef
  • Tegumental Ultrastructure of Adult Gynaecotyla squatarolae (Digenea: Microphallidae)
    Do-Seon Lim, Ki-Ju Choi, Sang-Mee Guk, Jong-Yil Chai, Il-Yong Park, Yun-Kyu Park, Min Seo
    The Korean Journal of Parasitology.2008; 46(2): 87.     CrossRef
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    Mohammed Hasan Ibraheem
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  • Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
    Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
    The Korean Journal of Parasitology.2004; 42(3): 129.     CrossRef
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    Woon-Mok Sohn, Min Seo, Jong-Yil Chai
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    Jong-Yil Chai, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee
    The Korean Journal of Parasitology.2002; 40(3): 107.     CrossRef
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  • Surface ultrastructure of Metagonimus takahashii metacercariae and adults
    Jong-Yil Chai, Sang-Mee Guk, Eun-Taek Han, Min Seo, Eun-Hee Shin, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee
    The Korean Journal of Parasitology.2000; 38(1): 9.     CrossRef
  • A cytogenetic study on human intestinal trematodes of the genus Metagonimus (Digenea: Heterophyidae) in Korea
    Soo-Ung Lee, Sun Huh, Gab-Man Park, Jong-Yil Chai
    The Korean Journal of Parasitology.1999; 37(4): 237.     CrossRef
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Discovery of Gymnophalloides seoi metacercariae in oysters from islands of the West Sea known as the habitats of paleartic oystercatchers
Woon-Mok Sohn, Yong-Suk Ryang, Jong-Yil Chai, Soon-Hyung Lee
Korean J Parasitol 1998;36(3):163-169.
Published online September 30, 1998
DOI: https://doi.org/10.3347/kjp.1998.36.3.163

An epidemiological survey was performed to know the infection status of oysters with Gymnophalloides seoi metacercariae in 7 islands of the West Sea known as the habitat of paleartic oystercatchers, Haematopus ostralegus osculans, in Korea. The surveyed areas were Aphaedo (Shinan-gun, Chollanam-do), Jangjado, Sonyudo and Munyodo (Okdo-myon, Kunsan-shi, Chollabuk-do), Yubudo (Changhang-up, Sochon-gun Chungchongnam-do), and Polumdo and Chumoondo (Sodo-myon, Kangwha-gun. Inchon-shi). The oysters collected from Aphaedo, the known endemic focus, were examined monthly from August 1995 to October 1996 for observation of any seasonal variation of the metacercarial density. The average metacercarial burden was 761-2,077 by month, but the seasonal variation of the metacercarial density was not obvious. A total of 54 metacercariae was detected in 63 oysters collected from Yubudo. Out of 30 oysters from Sonyudo, 25 (83.3%) were infected with 1-66 metacercariae (12.6 in average). All of 50 oysters (100%) from Munyodo were infected with 3-162 metacercariae (53.5 in average). Only 4 metacercariae were detected in 100 oysters from Chumoondo. However, no metacercariae were found in 55 oysters from Jangjado and 50 oysters from Polumdo. From the above results, it was confirmed that G. seoi is still highly prevalent in oysters from Aphaedo, and several islands of the West Sea known as the habitats of paleartic oystercatchers are new endemic areas of this fluke.

Citations

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  • Survey of Gymnophalloides seoi Metacercariae in Natural and Cultured Oysters from Several Western Coastal Areas, Korea
    Taehee Chang, Bong-Kwang Jung, Hyemi Song, Jaeeun Cho, Sooji Hong, Keon-Hoon Lee, Eui-Hyug Hoang, Jisu Kang, Jini Lim, Hana Lee, Jong-Yil Chai
    The Korean Journal of Parasitology.2019; 57(6): 705.     CrossRef
  • Prevalence and Density of Digenetic Trematode Metacercariae in Clams and Oysters from Western Coastal Regions of the Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Won-Ja Lee
    The Korean Journal of Parasitology.2017; 55(4): 399.     CrossRef
  • Foodborne Intestinal Flukes in Southeast Asia
    Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • A new endemic focus of Gymnophalloides seoi infection on Aphae Island, Shinan-gun, Jeollanam-do
    Jae-Hwan Park, Sang-Mee Guk, Eun-Hee Shin, Hyo-Jin Kim, Jae-Lip Kim, Min Seo, Yun-Kyu Park, Jong-Yil Chai
    The Korean Journal of Parasitology.2007; 45(1): 39.     CrossRef
  • Gymnophalloides seoi: a new human intestinal trematode
    Jong-Yil Chai, Min-Ho Choi, Jae-Ran Yu, Soon-Hyung Lee
    Trends in Parasitology.2003; 19(3): 109.     CrossRef
  • Food-borne intestinal trematode infections in the Republic of Korea
    Jong-Yil Chai, Soon-Hyung Lee
    Parasitology International.2002; 51(2): 129.     CrossRef
  • Intestinal histopathology and in situ postures of Gymnophalloides seoi in experimentally infected mice
    Jong-Yil Chai, Hong-Soon Lee, Sung-Jong Hong, Jae Hyung Yoo, Sang-Mee Guk, Min Seo, Min-Ho Choi, Soon-Hyung Lee
    The Korean Journal of Parasitology.2001; 39(1): 31.     CrossRef
  • A nationwide survey of the prevalence of human Gymnophalloides seoi infection on western and southern coastal islands in the Republic of Korea
    Jong-Yil Chai, Jae-Hwan Park, Eun-Taek Han, Eun-Hee Shin, Jae-Lip Kim, Kwang-Seon Hong, Han-Jong Rim, Soon-Hyung Lee
    The Korean Journal of Parasitology.2001; 39(1): 23.     CrossRef
  • A review of Gymnophalloides seoi (Digenea: Gymnophallidae) and human infections in the Republic of Korea
    Soon-Hyung Lee, Jong-Yil Chai
    The Korean Journal of Parasitology.2001; 39(2): 85.     CrossRef
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Two endemic foci of heterophyids and other intestinal fluke infections in southern and western coastal areas in Korea
Jong-Yil Chai, Tae-Eui Song, Eun-Taek Han, Sang-Mee Guk, Yun-Kyu Park, Min-Ho Choi, Soon-Hyung Lee
Korean J Parasitol 1998;36(3):155-161.
Published online September 30, 1998
DOI: https://doi.org/10.3347/kjp.1998.36.3.155

Two endemic foci of heterophyid infections were discovered in coastal villages of Puan-gun, Chollabuk-do, and Sachon-gun. Kyongsangnam-do, Korea. Fecal examinations were performed on 153 inhabitants of Puan-gun and 138 of Sachon-gun, using cellophane thick smear and formalin-ether sedimentation technique. The helminth egg and/or protozoan cyst positive rate was 21.5% (33/153) in Puan-gun and 39.1% (54/138) in Sachon-gun. In Puan-gun, the egg positive rate of heterophyids was the highest, 17.6%, and that of other parasites was 0.7-2.6% by parasite species. In Sachon-gun, that of heterophyids was 18.8%, followed by Clonorchis sinensis (12.3%), and other parasites (0.7-5.0%). Twenth-two (Puan-gun) and six (Sachon-gun) heterophyid egg positive cases were treated with praziquantel, and adult flukes were collected from their diarrheic stools. A total of 3,284 adult flukes of Heterophyes nocens was collected from all of the 22 patients treated in Puan-gun (3-778 individually), and other trematodes were also collected from 2-15 patients: Pygidiopsis summa, Stellantchasmus falcalus, Metagonimus yokogawai, M. miyatai, Stictodora fuscata, Heterophyopsis continua, Acanthoparyphium kurogamo, and Gymnophalloides seoi. In Sachon-gun, M. yokogawai (3,007 specimens), H. nocens (120), and S. falcatus (46) were collected from 5 of 6 treated patients, and H. continua and S. lari each from one patient. The present study revealed that heterophyid flukes, especially H. nocens and M. yokogawai, are prevalent in the southern and western coastal areas of Korea where fresh and/or brackish water fishes are popularly eaten raw.

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