Skip to main navigation Skip to main content
  • KSPTM
  • E-Submission

PHD : Parasites, Hosts and Diseases

OPEN ACCESS
Search
Advanced Search
ABOUT
BROWSE ARTICLES
FOR CONTRIBUTORS

Page Path

89
results for

"Toxoplasma gondii"

Article category

Keywords

More +

Publication year

More +

Authors

More +

Funded articles

More +

"Toxoplasma gondii"

Brief Communication

High Toxoplasma gondii Seropositivity among Brain Tumor Patients in Korea
Bong-Kwang Jung, Hyemi Song, Min-Jae Kim, Jaeeun Cho, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2016;54(2):201-204.
Published online April 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.2.201
Toxoplasma gondii is an intracellular protozoan that can modulate the environment of the infected host. An unfavorable environment modulated by T. gondii in the brain includes tumor microenvironment. Literature has suggested that T. gondii infection is associated with development of brain tumors. However, in Korea, epidemiological data regarding this correlation have been scarce. In this study, in order to investigate the relationship between T. gondii infection and brain tumor development, we investigated the seroprevalence of T. gondii among 93 confirmed brain tumor patients (various histological types, including meningioma and astrocytoma) in Korea using ELISA. The results revealed that T. gondii seropositivity among brain tumor patients (18.3%) was significantly (P<0.05) higher compared with that of healthy controls (8.6%). The seropositivity of brain tumor patients showed a significant age-tendency, i.e., higher in younger age group, compared with age-matched healthy controls (P<0.05). In conclusion, this study supports the close relationship between T. gondii infection and incidence of brain tumors.

Citations

Citations to this article as recorded by  Crossref logo
  • Relationship between parasites and lung cancer: Unveiling the link
    Mariam T. El Khadrawe, Nahla El Skhawy, Maha M. Eissa
    Tropical Medicine & International Health.2025; 30(7): 613.     CrossRef
  • Comprehensive analysis of Toxoplasma gondii migration routes and tissue dissemination in the host
    Carlos J. Ramírez-Flores, Ricardo Mondragón-Flores, Mehmet Aykur
    PLOS Neglected Tropical Diseases.2025; 19(7): e0013369.     CrossRef
  • Toxoplasma gondii: Seroprevalence and association with childhood brain tumors in Egypt
    Marwa M. Hamouda, Amany S. El-Saied, Ahmed Zaher, Amr Farid Khalil, Ayat A. ElBlihy, Nairmen Nabih, Samar N. El-Beshbishi
    Acta Tropica.2024; 251: 107123.     CrossRef
  • Whorling‐sclerosing meningioma invading skull bone and subcutaneous tissue with an incidental toxoplasmosis: A case report
    Zeina Alabbas, Yamani Tamer, Mhammad Jneidi, Rana Issa
    Neuropathology.2023; 43(3): 262.     CrossRef
  • Toxoplasma gondii infection in patients with brain tumors in Southern Iran: a case-control study
    Qasem Asgari, Farshad Rajabi, Fataneh Sajadian, Mohammad Saleh Bahreini, Nasir Arefkhah
    Journal of Parasitic Diseases.2023; 47(2): 291.     CrossRef
  • Differentiating Cerebral Toxoplasmosis and Tumor Recurrence by Thallium-201 Single-Photon Emission Computerized Tomography in a 28-Year-Old Female with Astrocytoma
    Mia Patrice Dela Vega, Paul Vincent Opinaldo, Julette Marie Batara
    Case Reports in Oncology.2023; : 372.     CrossRef
  • Toxoplasma gondii infection/exposure and the risk of brain tumors: A systematic review and meta-analysis
    Ali Abdollahi, Iman Razavian, Elnaz Razavian, Sahar Ghodsian, Mustafa Almukhtar, Elika Marhoommirzabak, Behnam Sartip, Hamid Parsa, Ali Rostami
    Cancer Epidemiology.2022; 77: 102119.     CrossRef
  • Toxoplasma gondii and Rickettsia spp. in ticks collected from migratory birds in the Republic of Korea
    A.-Tai Truong, Mi-Sun Yoo, Subin Min, Ji-Yeon Lim, Hyun-Ji Seo, Heung-Chul Kim, Sung-Tae Chong, Terry A. Klein, Chang-uk Park, Sook-Young Cho, Chang-Yong Choi, Young-Soo Kwon, Miran Kim, Soon-Seek Yoon, Yun Sang Cho
    Scientific Reports.2022;[Epub]     CrossRef
  • Serology for Toxoplasma in Immunocompromised Patients: Still Useful?
    Damien Dupont, Hélène Fricker-Hidalgo, Marie-Pierre Brenier-Pinchart, Cécile Garnaud, Martine Wallon, Hervé Pelloux
    Trends in Parasitology.2021; 37(3): 205.     CrossRef
  • Toxoplasma gondii infection and the risk of adult glioma in two prospective studies
    James M. Hodge, Anna E. Coghill, Youngchul Kim, Noemi Bender, Stephanie A. Smith‐Warner, Susan Gapstur, Lauren R. Teras, Tom K. Grimsrud, Tim Waterboer, Kathleen M. Egan
    International Journal of Cancer.2021; 148(10): 2449.     CrossRef
  • Imiquimod Targets Toxoplasmosis Through Modulating Host Toll-Like Receptor-MyD88 Signaling
    Maguy Hamie, Rania Najm, Carine Deleuze-Masquefa, Pierre Antoine Bonnet, Jean-François Dubremetz, Marwan El Sabban, Hiba El Hajj
    Frontiers in Immunology.2021;[Epub]     CrossRef
  • Infratentorial Stereotactic Biopsy of Brainstem and Cerebellar Lesions
    Jacek Furtak, Paulina Śledzińska, Marek G. Bebyn, Tadeusz Szylberg, Stanisław Krajewski, Marcin Birski, Marek Harat
    Brain Sciences.2021; 11(11): 1432.     CrossRef
  • Comprehensive Overview of Toxoplasma gondii-Induced and Associated Diseases
    Darine Daher, Ahmad Shaghlil, Eyad Sobh, Maguy Hamie, Malika Elhage Hassan, Mohamad Bahij Moumneh, Shaymaa Itani, Rana El Hajj, Lina Tawk, Marwan El Sabban, Hiba El Hajj
    Pathogens.2021; 10(11): 1351.     CrossRef
  • Molecular Detection of Toxoplasma Gondii in Haemaphysalis Ticks in Korea
    Ju Yeong Kim, You Shine Kwak, In-Yong Lee, Tai-Soon Yong
    The Korean Journal of Parasitology.2020; 58(3): 327.     CrossRef
  • Increased risk of Toxoplasma gondii infection in cancer patients: A meta-analysis of current evidence based on case-control study
    Lei Liu, Peng Wang, Jing Xu, Chao-Ming Xia
    Acta Tropica.2019; 192: 30.     CrossRef
  • Seroprevalence of Toxoplasma gondii infection in cancer patients: A systematic review and meta-analysis
    Davood Anvari, Mehdi Sharif, Shahabeddin Sarvi, Sargis A. Aghayan, Shirzad Gholami, Abdol Sattar Pagheh, Seyed Abdollah Hosseini, Reza Saberi, Tooran Nayeri Chegeni, Zahra Hosseininejad, Ahmad Daryani
    Microbial Pathogenesis.2019; 129: 30.     CrossRef
  • Seroprevalence and risk factors of Toxoplasma gondii infection in oral cancer patients in China: a case–control prospective study
    N. Zhou, X. Y. Zhang, Y. X. Li, L. Wang, L. L. Wang, W. Cong
    Epidemiology and Infection.2018; 146(15): 1891.     CrossRef
  • Puzzling and ambivalent roles of malarial infections in cancer development and progression
    ERIC FAURE
    Parasitology.2016; 143(14): 1811.     CrossRef
  • 10,669 View
  • 141 Download
  • 18 Web of Science
  • Crossref

Original Articles

In Vitro and in Vivo Effects of Nitrofurantoin on Experimental Toxoplasmosis
Seon-Ju Yeo, ChunMei Jin, SungYeon Kim, Hyun Park
Korean J Parasitol 2016;54(2):155-161.
Published online April 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.2.155
Toxoplasma gondii is an important opportunistic pathogen that causes toxoplasmosis, which has very few therapeutic treatment options. The most effective therapy is a combination of pyrimethamine and sulfadiazine; however, their utility is limited because of drug toxicity and serious side effects. For these reasons, new drugs with lower toxicity are urgently needed. In this study, the compound, (Z)-1-[(5-nitrofuran-2-yl)methyleneamino]-imidazolidine-2,4-dione (nitrofurantoin), showed anti-T. gondii effects in vitro and in vivo. In HeLa cells, the selectivity of nitrofurantoin was 2.3, which was greater than that of pyrimethamine (0.9). In T. gondii-infected female ICR mice, the inhibition rate of T. gondii growth in the peritoneal cavity was 44.7% compared to the negative control group after 4-day treatment with 100 mg/kg of nitrofurantoin. In addition, hematology indicators showed that T. gondii infection-induced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, biochemical parameters involved in liver injury, were reduced by nitrofurantoin significantly. Moreover, nitrofurantoin exerted significant effects on the index of antioxidant status, i.e., malondialdehyde (MDA) and glutathione (GSH). The nitrofurantoin-treated group inhibited the T. gondii-induced MDA levels while alleviating the decrease in GSH levels. Thus, nitrofurantoin is a potential anti-T. gondii candidate for clinical application.

Citations

Citations to this article as recorded by  Crossref logo
  • Extracts of food and medicinal plants sold in Moroccan markets induce apoptosis-like in Toxoplasma gondii tachyzoites in vitro
    Ismail Elkoraichi, Nathalie Moiré, Samira Rais, Isabelle Dimier-Poisson, Fouad Daoudi, Françoise Debierre-Grockiego
    Scientific African.2025; 27: e02529.     CrossRef
  • Efficacy of nitrofurantoin in treatment of murine model of trichinellosis
    Basma M. Elmansory, Hager S. Zoghroban, Dina M. El-Guindy, Dina A. El-Guindy
    Experimental Parasitology.2025; 277: 109022.     CrossRef
  • The Brazilian Toxoplasma gondii strain BRI caused greater inflammation and impairment in anxiogenic behavior in mice, which was reverted by rosuvastatin treatment
    Fernanda Ferreira Evangelista, Priscilla de Laet Sant’Ana, Willian Costa Ferreira, Thaisa Andreia Ferreira, Milena Lopes dos Santos, Amanda Hinobu de Souza, Felipe Aparecido Lacerda de Andrade, Douglas Aparecido da Silva, Luiz Daniel de Barros, Cristiane
    Parasitology Research.2024;[Epub]     CrossRef
  • Inclusion of Nitrofurantoin into the Realm of Cancer Chemotherapy via Biology-Oriented Synthesis and Drug Repurposing
    Perihan A. Elzahhar, Hisham A. Nematalla, Houssam Al-Koussa, Carla Abrahamian, Amira F. El-Yazbi, Larry Bodgi, Jolie Bou-Gharios, Joyce Azzi, Joelle Al Choboq, Hala F. Labib, Wassim Abou Kheir, Marwa M. Abu-Serie, Mohamed A. Elrewiny, Ahmed F. El-Yazbi, A
    Journal of Medicinal Chemistry.2023; 66(7): 4565.     CrossRef
  • Evaluation of mono and combined nitrofurantoin therapy for toxoplasmosis in vivo using murine model
    Asmaa Elkholy, Rita Wassef, Omnia Alsaid, Mona Elawady, Ashraf Barakat, Ashraf Soror, Shereen Kishik
    Pathogens and Global Health.2023; 117(7): 664.     CrossRef
  • Old Dogs with New Tricks: Antiparasitic Potential of Structurally Diverse 5-Nitrofuran and 5-Nitrothiophene Imines and Acyl Hydrazones
    Ibrahim S. Al Nasr, Waleed S. Koko, Tariq A. Khan, Rainer Schobert, Bernhard Biersack
    Scientia Pharmaceutica.2023; 91(3): 44.     CrossRef
  • Anti-Toxoplasma gondii agent isolated from Orostachys malacophylla (Pallas) Fischer
    Yan Piao, Lili Jin, Xu Cheng, Weifeng Yan, Changhao Zhang, Sihong Wang, Chunmei Jin
    Experimental Parasitology.2022; 242: 108397.     CrossRef
  • Recent Advances in the Synthesis and Development of Nitroaromatics as Anti-Infective Drugs
    Christina Kannigadu, David. D. N'Da
    Current Pharmaceutical Design.2020; 26(36): 4658.     CrossRef
  • Safety and efficacy of the bumped kinase inhibitor BKI-1553 in pregnant sheep experimentally infected with Neospora caninum tachyzoites
    Roberto Sánchez-Sánchez, Ignacio Ferre, Michela Re, Patricia Vázquez, Luis Miguel Ferrer, Javier Blanco-Murcia, Javier Regidor-Cerrillo, Manuel Pizarro Díaz, Marta González-Huecas, Enrique Tabanera, Paula García-Lunar, Julio Benavides, Pablo Castaño, Andr
    International Journal for Parasitology: Drugs and Drug Resistance.2018; 8(1): 112.     CrossRef
  • A Systematic Review of In vitro and In vivo Activities of Anti-Toxoplasma Drugs and Compounds (2006–2016)
    Mahbobeh Montazeri, Mehdi Sharif, Shahabeddin Sarvi, Saeed Mehrzadi, Ehsan Ahmadpour, Ahmad Daryani
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • Lectins from Synadenium carinatum (ScLL) and Artocarpus heterophyllus (ArtinM) Are Able to Induce Beneficial Immunomodulatory Effects in a Murine Model for Treatment of Toxoplasma gondii Infection
    Eliézer L. P. Ramos, Silas S. Santana, Murilo V. Silva, Fernanda M. Santiago, Tiago W. P. Mineo, José R. Mineo
    Frontiers in Cellular and Infection Microbiology.2016;[Epub]     CrossRef
  • 10,151 View
  • 129 Download
  • 12 Web of Science
  • Crossref
Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
Min Jae Kim, Bong-Kwang Jung, Jaeeun Cho, Hyemi Song, Kyung-Ho Pyo, Ji Min Lee, Min-Kyung Kim, Jong-Yil Chai
Korean J Parasitol 2016;54(2):147-154.
Published online April 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.2.147
Toxoplasma gondii infection induces alteration of the host cell cycle and cell proliferation. These changes are not only seen in directly invaded host cells but also in neighboring cells. We tried to identify whether this alteration can be mediated by exosomes secreted by T. gondii-infected host cells. L6 cells, a rat myoblast cell line, and RH strain of T. gondii were selected for this study. L6 cells were infected with or without T. gondii to isolate exosomes. The cellular growth patterns were identified by cell counting with trypan blue under confocal microscopy, and cell cycle changes were investigated by flow cytometry. L6 cells infected with T. gondii showed decreased proliferation compared to uninfected L6 cells and revealed a tendency to stay at S or G2/M cell phase. The treatment of exosomes isolated from T. gondii-infected cells showed attenuation of cell proliferation and slight enhancement of S phase in L6 cells. The cell cycle alteration was not as obvious as reduction of the cell proliferation by the exosome treatment. These changes were transient and disappeared at 48 hr after the exosome treatment. Microarray analysis and web-based tools indicated that various exosomal miRNAs were crucial for the regulation of target genes related to cell proliferation. Collectively, our study demonstrated that the exosomes originating from T. gondii could change the host cell proliferation and alter the host cell cycle.

Citations

Citations to this article as recorded by  Crossref logo
  • Human Brain Endothelial Cell-Derived Extracellular Vesicles Reduce Toxoplasma gondii Infection In Vitro in Human Brain and Umbilical Cord Vein Endothelial Cells
    Luiz Fernando Cardoso Garcia, Victoria Cruz Cavalari, Pryscilla Fanini Wowk, Letusa Albrecht
    International Journal of Molecular Sciences.2025; 26(6): 2640.     CrossRef
  • Extracellular vesicles and endothelial dysfunction in infectious diseases
    Linfang Zhang, Jingshu Chi, Hao Wu, Xiujuan Xia, Canxia Xu, Hong Hao, Zhenguo Liu
    Journal of Extracellular Biology.2024;[Epub]     CrossRef
  • Toxoplasma gondii Me49 and NED strains arrest host cell cycle progression and alter chromosome segregation in a strain-independent manner
    Lisbeth Rojas-Barón, Carlos Hermosilla, Anja Taubert, Zahady D. Velásquez
    Frontiers in Microbiology.2024;[Epub]     CrossRef
  • Toxoplasma gondii infection-induced host cellular DNA damage is strain-dependent and leads to the activation of the ATM-dependent homologous recombination pathway
    Lisbeth Rojas-Barón, Carlos Hermosilla, Anja Taubert, Zahady D. Velásquez
    Frontiers in Cellular and Infection Microbiology.2024;[Epub]     CrossRef
  • Toxoplasma gondii modulates the host cell cycle, chromosome segregation, and cytokinesis irrespective of cell type or species origin
    Lisbeth Rojas-Baron, Kira Senk, Carlos Hermosilla, Anja Taubert, Zahady D. Velásquez
    Parasites & Vectors.2024;[Epub]     CrossRef
  • Exosomes: Friends or Foes in Microbial Infections?
    Samane Teymouri, Maryam Pourhajibagher, Abbas Bahador
    Infectious Disorders - Drug Targets.2024;[Epub]     CrossRef
  • MicroRNA mmu-miR-511-5p: A promising Diagnostic Biomarker in Experimental Toxoplasmosis Using Different Strains and Infective Doses in Mice with Different Immune States Before and After Treatment
    Rasha Fadly Mady, Mona Mohamed El-Temsahy, Yasmine Amr Issa, Aya Saied Zaghloul, Safaa Ibrahim Khedr
    Acta Parasitologica.2024; 69(2): 1253.     CrossRef
  • Exosomes, and the potential for exosome‐based interventions against COVID‐19
    Abolfazl Rahmani, Ali Soleymani, Mustafa Almukhtar, Kimia Behzad Moghadam, Zahra Vaziri, Ali Hosein Tabar Kashi, Reza Adabi Firoozjah, Mehrdad Jafari Tadi, Maryam Zolfaghari Dehkharghani, Hadi Valadi, Ali Akbar Moghadamnia, Robin B. Gasser, Ali Rostami
    Reviews in Medical Virology.2024;[Epub]     CrossRef
  • Potential of extracellular vesicles in the pathogenesis, diagnosis and therapy for parasitic diseases
    Ana Acacia Sá Pinheiro, Ana Claudia Torrecilhas, Bruno Solano de Freitas Souza, Fernanda Ferreira Cruz, Herbert Leonel de Matos Guedes, Tadeu Diniz Ramos, Miqueias Lopes‐Pacheco, Celso Caruso‐Neves, Patricia R. M. Rocco
    Journal of Extracellular Vesicles.2024;[Epub]     CrossRef
  • Toxoplasma gondii-Derived Exosomes: A Potential Immunostimulant and Delivery System for Tumor Immunotherapy Superior to Toxoplasma gondii
    Lai-Xi Zhao, Qiong Sun, Chong Wang, Jia-Jia Liu, Xiao-Rong Yan, Meng-Ci Shao, Li Yu, Wen-Hua Xu, Rui Xu
    International Journal of Nanomedicine.2024; Volume 19: 12421.     CrossRef
  • Toxoplasma gondii infection induces early host cell cycle arrest and DNA damage in primary human host cells by a MYR1-dependent mechanism
    Zahady D. Velásquez, Lisbeth Rojas-Baron, Iván Conejeros, Carlos Hermosilla, Anja Taubert
    Communications Biology.2024;[Epub]     CrossRef
  • A Systematic Review of Apicomplexa Looking into Epigenetic Pathways and the Opportunity for Novel Therapies
    Yara de Oliveira Brandão, Marcelo Beltrão Molento
    Pathogens.2023; 12(2): 299.     CrossRef
  • Guidelines for the purification and characterization of extracellular vesicles of parasites
    Carmen Fernandez‐Becerra, Patrícia Xander, Daniel Alfandari, George Dong, Iris Aparici‐Herraiz, Irit Rosenhek‐Goldian, Mehrdad Shokouhy, Melisa Gualdron‐Lopez, Nicholy Lozano, Nuria Cortes‐Serra, Paula Abou Karam, Paula Meneghetti, Rafael Pedro Madeira, Z
    Journal of Extracellular Biology.2023;[Epub]     CrossRef
  • Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii
    Thomas Grochow, Britta Beck, Zaida Rentería-Solís, Gereon Schares, Pavlo Maksimov, Christina Strube, Lisa Raqué, Johannes Kacza, Arwid Daugschies, Simone A. Fietz
    Communications Biology.2023;[Epub]     CrossRef
  • Liquid Biopsy for Promising Non-invasive Diagnostic Biomarkers in Parasitic Infections
    Eylem Akdur Ozturk, Ayse Caner
    Acta Parasitologica.2022; 67(1): 1.     CrossRef
  • Mesenchymal Stromal/Stem Cells-Derived Exosomes as an Antimicrobial Weapon for Orodental Infections
    Nazanin Jafari, Arezoo Khoradmehr, Reza Moghiminasr, Mina Seyed Habashi
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Toxoplasmosis in Human and Animals Around the World. Diagnosis and Perspectives in the One Health Approach
    Rosangela Aparecida Müller de Barros, Ana Claudia Torrecilhas, Maria Aparecida Moraes Marciano, Monica Leszkowicz Mazuz, Vera Lucia Pereira-Chioccola, Blima Fux
    Acta Tropica.2022; 231: 106432.     CrossRef
  • Extracellular Vesicles in Trypanosoma cruzi Infection: Immunomodulatory Effects and Future Perspectives as Potential Control Tools against Chagas Disease
    Nuria Cortes-Serra, Melisa Gualdron-Lopez, Maria-Jesus Pinazo, Ana Claudia Torrecilhas, Carmen Fernandez-Becerra, Daniele Maria-Ferreira
    Journal of Immunology Research.2022; 2022: 1.     CrossRef
  • The Core Human MicroRNAs Regulated by Toxoplasma gondii
    Neelam Antil, Mohammad Arefian, Mrudula Kinarulla Kandiyil, Kriti Awasthi, Thottethodi Subrahmanya Keshava Prasad, Rajesh Raju
    MicroRNA.2022; 11(2): 163.     CrossRef
  • Eimeria bovis infections induce G1 cell cycle arrest and a senescence-like phenotype in endothelial host cells
    Zahady D. Velásquez, Sara López-Osorio, Daniel Waiger, Carolina Manosalva, Learta Pervizaj-Oruqaj, Susanne Herold, Carlos Hermosilla, Anja Taubert
    Parasitology.2021; 148(3): 341.     CrossRef
  • Immunization with extracellular vesicles excreted by Toxoplasma gondii confers protection in murine infection, activating cellular and humoral responses
    Marta Marques Maia, Allecineia Bispo da Cruz, Noemi Nosomi Taniwaki, Gislene Mitsue Namiyama, Ricardo Gava, Aparecida Helena Souza Gomes, Cristina Takami Kanamura, Maria Lourdes Peris Barbo, Vera Lucia Pereira-Chioccola
    International Journal for Parasitology.2021; 51(7): 559.     CrossRef
  • Recombinant Toxoplasma gondii Ribosomal Protein P2 Modulates the Functions of Murine Macrophages In Vitro and Provides Immunity against Acute Toxoplasmosis In Vivo
    Zhengqing Yu, Yujia Lu, Zhaoyi Liu, Muhammad Tahir Aleem, Junlong Liu, Jianxun Luo, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li
    Vaccines.2021; 9(4): 357.     CrossRef
  • Characterization of extracellular vesicles isolated from types I, II and III strains of Toxoplasma gondii
    Talita Motta Quiarim, Marta Marques Maia, Allecineia Bispo da Cruz, Noemi Nosomi Taniwaki, Gislene Mitsue Namiyama, Vera Lucia Pereira-Chioccola
    Acta Tropica.2021; 219: 105915.     CrossRef
  • Mechanical overload‐induced muscle‐derived extracellular vesicles promote adipose tissue lipolysis
    Ivan J. Vechetti, Bailey D. Peck, Yuan Wen, R. Grace Walton, Taylor R. Valentino, Alexander P. Alimov, Cory M. Dungan, Douglas W. Van Pelt, Ferdinand von Walden, Björn Alkner, Charlotte A. Peterson, John J. McCarthy
    The FASEB Journal.2021;[Epub]     CrossRef
  • Characterization of murine extracellular vesicles and Toxoplasma gondii infection
    Marta Marques Maia, Allecineia Bispo da Cruz, Ingrid de Siqueira Pereira, Noemi Nosomi Taniwaki, Gislene Mitsue Namiyama, Vera Lucia Pereira‐Chioccola
    Parasite Immunology.2021;[Epub]     CrossRef
  • Human extracellular vesicles and correlation with two clinical forms of toxoplasmosis
    Allecineia Bispo da Cruz, Marta Marques Maia, Ingrid de Siqueira Pereira, Noemi Nosomi Taniwaki, Gislene Mitsue Namiyama, João Paulo Marochi Telles, Jose Ernesto Vidal, Lígia Cosentino Junqueira Franco Spegiorin, Cinara Cássia Brandão de Mattos, Luiz Carl
    PLOS ONE.2020; 15(3): e0229602.     CrossRef
  • Besnoitia besnoiti–driven endothelial host cell cycle alteration
    Zahady D. Velásquez, Sara Lopez-Osorio, Learta Pervizaj-Oruqaj, Susanne Herold, Carlos Hermosilla, Anja Taubert
    Parasitology Research.2020; 119(8): 2563.     CrossRef
  • Induction of protection in mice against a respiratory challenge by a vaccine formulated with exosomes isolated from Chlamydia muridarum infected cells
    Sukumar Pal, Yeva Mirzakhanyan, Paul Gershon, Delia F. Tifrea, Luis M. de la Maza
    npj Vaccines.2020;[Epub]     CrossRef
  • Label-free characterization of biochemical changes within human cells under parasite attack using synchrotron based micro-FTIR
    Hany M. Elsheikha, Nashwa A. Elsaied, K. L. Andrew Chan, Chris Brignell, Mohammad S. R. Harun, Katia Wehbe, G. Cinquee
    Analytical Methods.2019; 11(19): 2518.     CrossRef
  • Extracellular Vesicle-Mediated Communication Within Host-Parasite Interactions
    Zhenyu Wu, Lingling Wang, Jiaying Li, Lifu Wang, Zhongdao Wu, Xi Sun
    Frontiers in Immunology.2019;[Epub]     CrossRef
  • The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens
    Chelsea N. Davis, Helen Phillips, John J. Tomes, Martin T. Swain, Toby J. Wilkinson, Peter M. Brophy, Russell M. Morphew, Gabriel Rinaldi
    PLOS Neglected Tropical Diseases.2019; 13(2): e0007191.     CrossRef
  • Impact of Toxoplasma gondii Infection on Host Non-coding RNA Responses
    Kayla L. Menard, Breanne E. Haskins, Eric Y. Denkers
    Frontiers in Cellular and Infection Microbiology.2019;[Epub]     CrossRef
  • Extracellular vesicles in host-pathogen interactions and immune regulation — exosomes as emerging actors in the immunological theater of pregnancy
    Valéria de Lima Kaminski, Joel Henrique Ellwanger, José Artur Bogo Chies
    Heliyon.2019; 5(8): e02355.     CrossRef
  • Colon cancer cells secrete exosomes to promote self-proliferation by shortening mitosis duration and activation of STAT3 in a hypoxic environment
    Ruixue Ren, Hua Sun, Cui Ma, Jiatao Liu, Hua Wang
    Cell & Bioscience.2019;[Epub]     CrossRef
  • Toxoplasma gondii-induced host cellular cell cycle dysregulation is linked to chromosome missegregation and cytokinesis failure in primary endothelial host cells
    Zahady D. Velásquez, Iván Conejeros, Camilo Larrazabal, Katharina Kerner, Carlos Hermosilla, Anja Taubert
    Scientific Reports.2019;[Epub]     CrossRef
  • Extracellular vesicles from the aqueous humor of patients with uveitis
    CarmenLuz Pessuti, DeiseFialho Costa, KleberS Ribeiro, Heloisa Nascimento, Rubens Belfort, AlessandraG Commodaro, AnaClaudia Torrecilhas
    The Pan-American Journal of Ophthalmology.2019; 1(1): 1.     CrossRef
  • Quantitative proteomic analysis and functional characterization of Acanthamoeba castellanii exosome-like vesicles
    Wei-Chen Lin, Chia-Yun Tsai, Jian-Ming Huang, Shang-Rung Wu, Lichieh Julie Chu, Kuo-Yang Huang
    Parasites & Vectors.2019;[Epub]     CrossRef
  • Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile
    Paloma de Carvalho Vieira, Mariana Caldas Waghabi, Daniela Gois Beghini, Danilo Predes, Jose Garcia Abreu, Vincent Mouly, Gillian Butler-Browne, Helene Santos Barbosa, Daniel Adesse
    Frontiers in Cellular and Infection Microbiology.2019;[Epub]     CrossRef
  • Exosomes in Pathogen Infections: A Bridge to Deliver Molecules and Link Functions
    Wenchao Zhang, Xiaofeng Jiang, Jinghui Bao, Yi Wang, Huixing Liu, Lijun Tang
    Frontiers in Immunology.2018;[Epub]     CrossRef
  • Extracellular vesicles as mediators of immunopathology in infectious diseases
    Elham Hosseini‐Beheshti, Georges Emile Raymond Grau
    Immunology & Cell Biology.2018; 96(7): 694.     CrossRef
  • A new landscape of host–protozoa interactions involving the extracellular vesicles world
    Bruno Gavinho, Izadora Volpato Rossi, Ingrid Evans-Osses, Jameel Inal, Marcel I. Ramirez
    Parasitology.2018; 145(12): 1521.     CrossRef
  • Extracellular vesicles isolated from Toxoplasma gondii induce host immune response
    Valeria Oliveira Silva, Marta Marques Maia, Ana Claudia Torrecilhas, Noemi Nosomi Taniwaki, Gislene Mitsue Namiyama, Katia Cristina Oliveira, Kleber Silva Ribeiro, Maytê dos Santos Toledo, Patricia Xander, Vera Lucia Pereira‐Chioccola
    Parasite Immunology.2018;[Epub]     CrossRef
  • 11,515 View
  • 181 Download
  • 41 Web of Science
  • Crossref
Sterculic Acid and Its Analogues Are Potent Inhibitors of Toxoplasma gondii
Pan Hao, Intisar Q. M. Alaraj, Juma’a R. Al Dulayymi, Mark S. Baird, Jing Liu, Qun Liu
Korean J Parasitol 2016;54(2):139-145.
Published online April 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.2.139
Toxoplasmosis is a serious disease caused by Toxoplasma gondii, one of the most widespread parasites in the world. Lipid metabolism is important in the intracellular stage of T. gondii. Stearoyl-CoA desaturase (SCD), a key enzyme for the synthesis of unsaturated fatty acid is predicted to exist in T. gondii. Sterculic acid has been shown to specifically inhibit SCD activity. Here, we examined whether sterculic acid and its methyl ester analogues exhibit anti-T. gondii effects in vitro. T. gondii-infected Vero cells were disintegrated at 36 hr because of the propagation and egress of intracellular tachyzoites. All test compounds inhibited tachyzoite propagation and egress, reducing the number of ruptured Vero cells by the parasites. Sterculic acid and the methyl esters also inhibited replication of intracellular tachyzoites in HFF cells. Among the test compounds, sterculic acid showed the most potent activity against T. gondii, with an EC50 value of 36.2 μM, compared with EC50 values of 248-428 μM for the methyl esters. Our study demonstrated that sterculic acid and its analogues are effective in inhibition of T. gondii growth in vitro, suggesting that these compounds or analogues targeting SCD could be effective agents for the treatment of toxoplasmosis.

Citations

Citations to this article as recorded by  Crossref logo
  • Fungal Δ9-fatty acid desaturase: a unique enzyme at the core of lipid metabolism in Aspergillus fumigatus and a promising target for the search for antifungal strategies
    Jonatas Erick Maimoni Campanella, Leonardo Talachia Rosa, Iran Malavazi, Marcio Rodrigues
    mBio.2025;[Epub]     CrossRef
  • Trithiolato-bridged dinuclear ruthenium(II)-arene conjugates tethered with lipophilic units: Synthesis and Toxoplasma gondii antiparasitic activity
    Oksana Desiatkina, Nicoleta Anghel, Ghalia Boubaker, Yosra Amdouni, Andrew Hemphill, Julien Furrer, Emilia Păunescu
    Journal of Organometallic Chemistry.2023; 986: 122624.     CrossRef
  • Rare fatty acids and lipids in plant oilseeds: occurrence and bioactivity
    P. Avato, A. Tava
    Phytochemistry Reviews.2022; 21(2): 401.     CrossRef
  • Update on food sources and biological activity of odd-chain, branched and cyclic fatty acids –– A review
    Grzegorz Dąbrowski, Iwona Konopka
    Trends in Food Science & Technology.2022; 119: 514.     CrossRef
  • Sterculic Acid: The Mechanisms of Action beyond Stearoyl-CoA Desaturase Inhibition and Therapeutic Opportunities in Human Diseases
    Rafael Peláez, Ana Pariente, Álvaro Pérez-Sala, Ignacio M. Larráyoz
    Cells.2020; 9(1): 140.     CrossRef
  • Genome-Wide Transcriptomic Analysis Identifies Pathways Regulated by Sterculic Acid in Retinal Pigmented Epithelium Cells
    Ana Pariente, Álvaro Pérez-Sala, Rodrigo Ochoa, Rafael Peláez, Ignacio M. Larráyoz
    Cells.2020; 9(5): 1187.     CrossRef
  • Inhibition of HIV‐1 Protease by Carpobrotus edulis (L.)
    Beauty E. Omoruyi, David I. Ighodaro, Anthony J. Afolayan, Graeme Bradley, Yuan Xu
    Evidence-Based Complementary and Alternative Medicine.2020;[Epub]     CrossRef
  • The Mechanism of Action of Ursolic Acid as a Potential Anti-Toxoplasmosis Agent, and Its Immunomodulatory Effects
    Won Hyung Choi, In Ah Lee
    Pathogens.2019; 8(2): 61.     CrossRef
  • Anti-Toxoplasma gondii effect of two spider venoms in vitro and in vivo
    Shengjie Hou, Yuan Liu, Yaqin Tang, Mengqi Wu, Jianwu Guan, Xianyao Li, Zheng Wang, Jinying Jiang, Meichun Deng, Zhigui Duan, Xing Tang, Xiaoqing Han, Liping Jiang
    Toxicon.2019; 166: 9.     CrossRef
  • Identification and characterization of stearoyl-CoA desaturase in <italic>Toxoplasma gondii</italic>
    Pan Hao, Xia Cui, Jing Liu, Muzi Li, Yong Fu, Qun Liu
    Acta Biochimica et Biophysica Sinica.2019; 51(6): 614.     CrossRef
  • 3- O -Methyl-Alkylgallates Inhibit Fatty Acid Desaturation in Mycobacterium tuberculosis
    Nidja Rehberg, Edwin Omeje, Sherif S. Ebada, Lasse van Geelen, Zhen Liu, Parichat Sureechatchayan, Matthias U. Kassack, Thomas R. Ioerger, Peter Proksch, Rainer Kalscheuer
    Antimicrobial Agents and Chemotherapy.2019;[Epub]     CrossRef
  • Triclosan inhibits the growth of Neospora caninum in vitro and in vivo
    Heng Zhang, Jing Liu, Congshan Yang, Yong Fu, Jianhai Xu, Qun Liu
    Parasitology Research.2019; 118(10): 3001.     CrossRef
  • Lipidated cyclopropenes via a stable 3-N spirocyclopropene scaffold
    Pratik Kumar, Ting Jiang, Omar Zainul, Alyssa N. Preston, Sining Li, Joshua D. Farr, Pavit Suri, Scott T. Laughlin
    Tetrahedron Letters.2018; 59(37): 3435.     CrossRef
  • Molecular target analysis of stearoyl-CoA desaturase genes of protozoan parasites
    He Lu, Xin Qin, Jing Zhang, Shuang Zhang, Yu Zhu, Wei Hua Wu
    Acta Parasitologica.2018; 63(1): 48.     CrossRef
  • 10,449 View
  • 130 Download
  • 15 Web of Science
  • Crossref

Brief Communication

Seroprevalence of Toxoplasma gondii Infection among HIV/AIDS Patients in Eastern China
Guoqiang Shen, Xiaoming Wang, Hui Sun, Yaying Gao
Korean J Parasitol 2016;54(1):93-96.
Published online February 26, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.1.93
Toxoplasmosis, a neglected tropical disease caused by the protozoan parasite Toxoplasma gondii, occurs throughout the world. Human T. gondii infection is asymptomatic in 80% of the population; however, the infection is life-threatening and causes substantial neurologic damage in immunocompromised patients such as HIV-infected persons. The major purpose of this study was to investigate the seroprevalence of T. gondii infection in subjects infected with HIV/AIDS in eastern China. Our findings showed 9.7% prevalence of anti-T. gondii IgG antibody in HIV/AIDS patients, which was higher than in intravenous drug users (2.2%) and healthy controls (4.7%), while no significant difference was observed in the seroprevalence of anti-Toxoplasma IgM antibody among all participants (P>0.05). Among all HIV/AIDS patients, 15 men (7.7%) and 10 women (15.9%) were positive for anti-T. gondii IgG antibody; however, no significant difference was detected in the seroprevalence of anti-Toxoplasma IgG antibody between males and females. The frequency of anti-Toxoplasma IgG antibody was 8.0%, 13.2%, 5.5%, and 0% in patients with normal immune function (CD4+ T-lymphocyte count ≥500 cells/ml), immunocompromised patients (cell count ≥200 and <500 cells/ml), severely immunocompromised patients (cell count ≥50 and <200 cells/ml), and advanced AIDS patients, respectively (cell count <50 cells/ml), while only 3 immunocompromised patients were positive for anti-T. gondii IgM antibody. The results indicate a high seroprevalence of T. gondii infection in HIV/AIDS patients in eastern China, and a preventive therapy for toxoplasmosis may be given to HIV/AIDS patients based on CD4+ T lymphocyte count.

Citations

Citations to this article as recorded by  Crossref logo
  • The Global Prevalence of and Factors Associated with Parasitic Coinfection in People Living with Viruses: A Systematic Review and Meta-Analysis
    Yan Ge, Huaman Liu, Ningjun Ren, Abdul Qadeer, Ian Kim B. Tabios, Ian Kendrich C. Fontanilla, Lydia R. Leonardo, Banchob Sripa, Guofeng Cheng
    Pathogens.2025; 14(6): 534.     CrossRef
  • Seroprevalence and Risk Factors for Toxoplasma gondii Infection in People Living with HIV: A Cross-Sectional Study from Maputo Central Hospital, Mozambique
    Leonardo Manuel, Isac Comia, Regina Miambo, Irina Sousa, Nelson Cuboia, Awa Carimo, Sara Massuanganhe, Titos Buene, Lucas Banze, Belmiro Paraque, Noémia Nhancupe, Robert Schooley, Gabriela Santos-Gomes, Emília Noormahomed, Constance Benson
    Research and Reports in Tropical Medicine.2025; Volume 16: 43.     CrossRef
  • Impacto da imunossupressão pelo HIV na reativação da toxoplasmose: aspectos imunopatológicos, desafios diagnósticos e implicações clínicas
    Beatriz Ammar Roque, Fernanda Simoneto, Juliana Cogo
    RCMOS - Revista Científica Multidisciplinar O Saber.2025;[Epub]     CrossRef
  • Lactobacillus plantarum antimicrobial peptide Y8-2: A new strategy against Toxoplasma gondii
    Wenhui Guo, Jun Guo, Zixuan Wang, Pufang Liu, Jiashan Qin, Chao Duan, Xinyu Xue, Tieyun Chen, Xinlei Yan
    LWT.2024; 213: 117026.     CrossRef
  • Seroprevalencia y factores de riesgo de Toxoplasma gondii en Personas que Viven con VIH/Sida (PVVS) en el departamento de Cochabamba, Bolivia
    Bertha Alanoca, Tania Vargas Rivero, Gustavo Allende Larrain, Amílcar A. Flores Leon
    Gaceta Médica Boliviana.2023; 46(2): 62.     CrossRef
  • Seroprevalencia y factores de riesgo de Toxoplasma gondii en Personas que Viven con VIH/SIDA (PVVS) en el departamento de Cochabamba, Bolivia
    Amilcar Alejandro Flores Leon, Bertha Alanoca, Tania Vargas Rivero, Gustavo Allende Larrain
    Gaceta Médica Boliviana.2023; 46(2): 63.     CrossRef
  • Primary Toxoplasma gondii infection-associated with hemophagocytic syndrome in a man with HIV infection: a case report
    Ying Zhou, Yongfeng Liu, Ying Wen
    BMC Infectious Diseases.2022;[Epub]     CrossRef
  • Seronegative Infection with Toxoplasma gondii in Asymptomatic Human Immunodeficiency Virus Type 1 (HIV-1)-Infected Patients and in Blood Donors
    Agnieszka Pawełczyk, Małgorzata Bednarska, Kamila Caraballo Cortés, Marianna Glamkowska-Sady, Justyna Kowalska, Beata Uszyńska-Kałuża, Marek Radkowski, Renata Welc-Falęciak
    Journal of Clinical Medicine.2022; 11(3): 638.     CrossRef
  • Effect of some plant extracts from Egyptian herbal plants against Toxoplasma gondii tachyzoites in vitro
    Sara T. ELAZAB, Amal F. SOLIMAN, Yoshifumi NISHIKAWA
    Journal of Veterinary Medical Science.2021; 83(1): 100.     CrossRef
  • Molecular investigation of Toxoplasma gondii in oysters (Crassostrea spp.) sold on beaches in the State of Pará, Brazil
    Marcely Karen Santos do Rosário, Jacqueline Silva, Hugo Filipe Rodrigues Melo, Thamillys Rayssa Marques Monteiro, Davi Ribeiro da Costa, Alessandra Scofield, Carla Cristina Guimarães de Moraes
    Revista Brasileira de Parasitologia Veterinária.2021;[Epub]     CrossRef
  • Serological Evaluation of Toxoplasmosis and Related Risk Factors Among HIV+/AIDS Patients in Northern Iran
    Hasan Rafati-Sajedi, Bijan Majidi-Shad, Reza Jafari-Shakib, Zahra Atrkar-Roshan, Mohammad Reza Mahmoudi, Seyed Mahmood Rezvani
    Acta Parasitologica.2021; 66(4): 1417.     CrossRef
  • Determination of parasitic burden in the brain tissue of infected mice in acute toxoplasmosis after treatment by fluconazole combined with sulfadiazine and pyrimethamine
    Sekandarpour Sina, Jafari Modrek Mohammad, Shafiei Reza, Mohammadiha Anita, Etemadi Soudabeh, Mirahmadi Hadi
    European Journal of Medical Research.2021;[Epub]     CrossRef
  • Anti-Toxoplasmic Immunoglobulin G Quantitation Correlates with Immunovirological Parameters of HIV-Infected Cameroonians
    Aude Christelle Ka’e, Samuel Martin Sosso, Joseph Fokam, Rachel Kamgaing Simo, Sara Riwom Essama, Alex Durand Nka, Bouba Yagai, Cédric Joël Ninsu Dzukou, Michel Carlos Tommo Tchouaket, Collins Chenwi, Aissatou Abba, Nadine Fainguem, Marie Krystel Nnomo Za
    Current HIV Research.2021; 19(1): 73.     CrossRef
  • Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea
    Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho
    The Korean Journal of Parasitology.2020; 58(3): 257.     CrossRef
  • Seroprevalence of Toxoplasma gondii in HIV-infected patients admitted to a university hospital
    Pinar ERGEN, Fatma YILMAZ-KARADAĞ, Özlem AYDIN
    Journal of Health Sciences and Medicine.2020; 3(4): 372.     CrossRef
  • A Large-Scale Serological Survey of Toxoplasma gondii Infection Among Persons Participated in Health Screening in Yunnan Province, Southwestern China
    Zhao Li, Chun-Yi Sun, Ren-Dan Yang, Chang-Jun Xu, Mei-Chen Chen, Yun-Jiang Zhang, Wei Cong, Xing-Quan Zhu, Yang Liu, Feng-Cai Zou
    Vector-Borne and Zoonotic Diseases.2019; 19(6): 441.     CrossRef
  • Prevalence and burden of Toxoplasma gondii infection in HIV-infected people: a systematic review and meta-analysis
    Ze-Dong Wang, Shu-Chao Wang, Huan-Huan Liu, Hong-Yu Ma, Zhong-Yu Li, Feng Wei, Xing-Quan Zhu, Quan Liu
    The Lancet HIV.2017; 4(4): e177.     CrossRef
  • Toxoplasma gondii Infection in Immunocompromised Patients: A Systematic Review and Meta-Analysis
    Ze-Dong Wang, Huan-Huan Liu, Zhan-Xi Ma, Hong-Yu Ma, Zhong-Yu Li, Zhi-Bin Yang, Xing-Quan Zhu, Bin Xu, Feng Wei, Quan Liu
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • Evaluation of potential anti-toxoplasmosis efficiency of combined traditional herbs in a mouse model
    Xun-hui Zhuo, Hong-chao Sun, Bin Huang, Hai-jie Yu, Ying Shan, Ai-fang Du
    Journal of Zhejiang University-SCIENCE B.2017; 18(6): 453.     CrossRef
  • A Human Proteome Array Approach to Identifying Key Host Proteins Targeted by Toxoplasma Kinase ROP18
    Zhaoshou Yang, Yongheng Hou, Taofang Hao, Hee-Sool Rho, Jun Wan, Yizhao Luan, Xin Gao, Jianping Yao, Aihua Pan, Zhi Xie, Jiang Qian, Wanqin Liao, Heng Zhu, Xingwang Zhou
    Molecular & Cellular Proteomics.2017; 16(3): 469.     CrossRef
  • Elevated seroprevalence of Toxoplasma gondii in AIDS/HIV patients: A meta-analysis
    Lei Liu, Li-Na Liu, Peng Wang, Tian-Tian Lv, Yin-Guang Fan, Hai-Feng Pan
    Acta Tropica.2017; 176: 162.     CrossRef
  • 12,097 View
  • 151 Download
  • 19 Web of Science
  • Crossref

Original Articles

Afatinib Reduces STAT6 Signaling of Host ARPE-19 Cells Infected with Toxoplasma gondii
Zhaoshou Yang, Hye-Jin Ahn, Young-Hoon Park, Ho-Woo Nam
Korean J Parasitol 2016;54(1):31-38.
Published online February 26, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.1.31
Specific gene expressions of host cells by spontaneous STAT6 phosphorylation are major strategy for the survival of intracellular Toxoplasma gondii against parasiticidal events through STAT1 phosphorylation by infection provoked IFN-γ. We determined the effects of small molecules of tyrosine kinase inhibitors (TKIs) on the growth of T. gondii and on the relationship with STAT1 and STAT6 phosphorylation in ARPE-19 cells. We counted the number of T. gondii RH tachyzoites per parasitophorous vacuolar membrane (PVM) after treatment with TKIs at 12-hr intervals for 72 hr. The change of STAT6 phosphorylation was assessed via western blot and immunofluorescence assay. Among the tested TKIs, Afatinib (pan ErbB/EGFR inhibitor, 5 ?M) inhibited 98.0% of the growth of T. gondii, which was comparable to pyrimethamine (5 ?M) at 96.9% and followed by Erlotinib (ErbB1/EGFR inhibitor, 20 ?M) at 33.8% and Sunitinib (PDGFR or c-Kit inhibitor, 10 ?M) at 21.3%. In the early stage of the infection (2, 4, and 8 hr after T. gondii challenge), Afatinib inhibited the phosphorylation of STAT6 in western blot and immunofluorescence assay. Both JAK1 and JAK3, the upper hierarchical kinases of cytokine signaling, were strongly phosphorylated at 2 hr and then disappeared entirely after 4 hr. Some TKIs, especially the EGFR inhibitors, might play an important role in the inhibition of intracellular replication of T. gondii through the inhibition of the direct phosphorylation of STAT6 by T. gondii.

Citations

Citations to this article as recorded by  Crossref logo
  • Small molecule kinase inhibitor altiratinib inhibits brain cyst forming bradyzoites of Toxoplasma gondii
    Yeong Hoon Kim, Hye-Jin Ahn, Hwa Sun Kim, Ho-Woo Nam
    Journal of Microbiology.2025; 63(2): e2409001.     CrossRef
  • iTRAQ-Based Phosphoproteomic Analysis Exposes Molecular Changes in the Small Intestinal Epithelia of Cats after Toxoplasma gondii Infection
    Bintao Zhai, Yu-Meng Meng, Shi-Chen Xie, Jun-Jie Peng, Yang Liu, Yanhua Qiu, Lu Wang, Jiyu Zhang, Jun-Jun He
    Animals.2023; 13(22): 3537.     CrossRef
  • Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors
    Hye-Jung Kim, Hye-Jin Ahn, Hyeweon Kang, Jaehui Park, Seul gi Oh, Saehae Choi, Won-Kyu Lee, Ho-Woo Nam
    The Korean Journal of Parasitology.2020; 58(3): 249.     CrossRef
  • A Human Proteome Array Approach to Identifying Key Host Proteins Targeted by Toxoplasma Kinase ROP18
    Zhaoshou Yang, Yongheng Hou, Taofang Hao, Hee-Sool Rho, Jun Wan, Yizhao Luan, Xin Gao, Jianping Yao, Aihua Pan, Zhi Xie, Jiang Qian, Wanqin Liao, Heng Zhu, Xingwang Zhou
    Molecular & Cellular Proteomics.2017; 16(3): 469.     CrossRef
  • Adverse Event Profile of Pyrimethamine-Based Therapy in Toxoplasmosis: A Systematic Review
    Ruben R. Ben-Harari, Elizabeth Goodwin, Julio Casoy
    Drugs in R&D.2017; 17(4): 523.     CrossRef
  • Suppressors for Human Epidermal Growth Factor Receptor 2/4 (HER2/4): A New Family of Anti-Toxoplasmic Agents in ARPE-19 Cells
    Yeong Hoon Kim, Lokraj Bhatt, Hye-Jin Ahn, Zhaoshou Yang, Won-Kyu Lee, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(5): 491.     CrossRef
  • 10,345 View
  • 103 Download
  • 6 Web of Science
  • Crossref
A Novel Polyclonal Antiserum against Toxoplasma gondii Sodium Hydrogen Exchanger 1
Bin Xiao, Zhenzhan Kuang, Yanli Zhan, Daxiang Chen, Yang Gao, Ming Li, Shuhong Luo, Wenbo Hao
Korean J Parasitol 2016;54(1):21-29.
Published online February 26, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.1.21
The sodium hydrogen exchanger 1 (NHE1), which functions in maintaining the ratio of Na+ and H+ ions, is widely distributed in cell plasma membranes. It plays a prominent role in pH balancing, cell proliferation, differentiation, adhesion, and migration. However, its exact subcellular location and biological functions in Toxoplasma gondii are largely unclear. In this study, we cloned the C-terminal sequence of T. gondii NHE1 (TgNHE1) incorporating the C-terminal peptide of NHE1 (C-NHE1) into the pGEX4T-1 expression plasmid. The peptide sequence was predicted to have good antigenicity based on the information obtained from an immune epitope database. After induction of heterologous gene expression with isopropyl-b-D-thiogalactoside, the recombinant C-NHE1 protein successfully expressed in a soluble form was purified by glutathione sepharose beads as an immunogen for production of a rabbit polyclonal antiserum. The specificity of this antiserum was confirmed by western blotting and immunofluorescence. The antiserum could reduce T. gondii invasion into host cells, indicated by the decreased TgNHE1 expression in T. gondii parasites that were pre-incubated with antiserum in the process of cell entry. Furthermore, the antiserum reduced the virulence of T. gondii parasites to host cells in vitro, possibly by blocking the release of Ca2+. In this regard, this antiserum has potential to be a valuable tool for further studies of TgNHE1.

Citations

Citations to this article as recorded by  Crossref logo
  • Identification and Characterization of a Cleavage Site in the Proteolysis of Orf Virus 086 Protein
    Xiaoping Wang, Bin Xiao, Jiafeng Zhang, Daxiang Chen, Wei Li, Ming Li, Wenbo Hao, Shuhong Luo
    Frontiers in Microbiology.2016;[Epub]     CrossRef
  • 9,507 View
  • 87 Download
  • 1 Web of Science
  • Crossref

Brief Communications

Seroprevalence of Encephalitozoon cuniculi and Toxoplasma gondii in domestic rabbits (Oryctolagus cuniculus) in China
Qing-Feng Meng, Wei-Lin Wang, Xiao-Ting Ni, Hai-Bin Li, Gui-Zhe Yao, Xiao-Lin Sun, Wei-Li Wang, Wei Cong
Korean J Parasitol 2015;53(6):759-763.
Published online December 31, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.6.759
The breeding of domestic rabbits (Oryctolagus cuniculus) for human consumption has a long tradition in China. Infections that can affect the production of meat or even be transmitted from animals to humans are important to monitor, especially for public health reasons as well as for their impact on animal health. Thus, a total of 1,132 domestic rabbit sera from 4 regions in China were collected for serological screening for Encephalitozoon cuniculi and for Toxoplasma gondii by ELISA and modified agglutination test (MAT), respectively. Antibodies to E. cuniculi were detected in 248/1,132 (21.9%) sera tested while antibodies against T. gondii revealed a seroprevalence of 51/1,132 (4.5%). We believe that the present results are of epidemiological implications and public health importance due to the acknowledged susceptibility of humans to E. cuniculi and T. gondii infections. Therefore, routine screening tests of domestic rabbits are proposed considering the zoonotic potential of these parasites.

Citations

Citations to this article as recorded by  Crossref logo
  • The Prevalence of Encephalitozoon cuniculi in Domestic Rabbits (Oryctolagus cuniculus) in the North-Western Region of Romania Using Serological Diagnosis: A Preliminary Study
    Anca-Alexandra Doboși, Anamaria Ioana Paștiu, Lucia-Victoria Bel, Dana Liana Pusta
    Microorganisms.2024; 12(7): 1440.     CrossRef
  • Serological survey for antibodies to Encephalitozoon cuniculi and Toxoplasma gondii in pet rabbits in eastern coastal areas of China
    Yong WANG, Xuling QIN, Xiuguo DIAO, Yongxia LIU, Jianzhu LIU
    Journal of Veterinary Medical Science.2022; 84(6): 777.     CrossRef
  • Epidemiological and Public Health Significance of Toxoplasma gondii Infection in Wild Rabbits and Hares: 2010–2020
    Sonia Almeria, Fernando H. A. Murata, Camila K. Cerqueira-Cézar, Oliver C. H. Kwok, Alicia Shipley, Jitender P. Dubey
    Microorganisms.2021; 9(3): 597.     CrossRef
  • Comparison of Seroprevalence and PCR Results in the Detection of Toxoplasma gondii in Pet Rabbits in Poland
    Anna Woźniak-Biel, Aleksandra Podolak
    Vector-Borne and Zoonotic Diseases.2020; 20(4): 281.     CrossRef
  • Surveillance of Microsporidia and Protozoan Pathogens in Pensacola Florida: A One‐year Study
    Joseph A. Moss, Richard A. Snyder
    Journal of Eukaryotic Microbiology.2019; 66(4): 617.     CrossRef
  • Relationship between seropositivity of Encephalitozoon cuniculi and renal biochemical markers in clinically healthy rabbits
    Özcan ÖZKAN, Banuçiçek YÜCESAN, Selçuk PEKKAYA, Mehmet Eray ALÇIĞIR, İsmayil Safa GÜRCAN
    Ankara Üniversitesi Veteriner Fakültesi Dergisi.2019; 66(2): 197.     CrossRef
  • Occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in slaughtered domestic rabbits in central China
    Weifeng Qian, Wenchao Yan, Chaochao Lv, Rongzhen Bai, Tianqi Wang
    Parasite.2019; 26: 36.     CrossRef
  • Seroprevalence and risk factors of Toxoplasma gondii infection in rabbit of local Algerian population
    Mina Henneb, Khaled Harhoura, Mohamed Amine Bekara, Safia Zenia, Miriem Aissi
    Veterinary World.2019; 12(6): 855.     CrossRef
  • Seroprevalence of Toxoplasma gondii and Encephalitozoon cuniculi among domestic rabbits in central China
    Shuai Wang, Zhijun Yao, Lingjuan Li, Yaoqian Pan, Pengju Li, Xiaoxu Nan, Qing Xie, Zhenchao Zhang
    Parasite.2018; 25: 9.     CrossRef
  • Rapid detection of three rabbit pathogens by use of the Luminex x-TAG assay
    Miaoli Wu, Yujun Zhu, Feng Cong, Dan Rao, Wen Yuan, Jing Wang, Bihong Huang, Yuexiao Lian, Yu Zhang, Ren Huang, Pengju Guo
    BMC Veterinary Research.2018;[Epub]     CrossRef
  • 9,575 View
  • 108 Download
  • 10 Web of Science
  • Crossref
Chronic Toxoplasmosis Modulates the Induction of Contact Hypersensitivity by TNCB in Mouse Model
Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
Korean J Parasitol 2015;53(6):755-757.
Published online December 31, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.6.755
Mouse models of chronic toxoplasmosis and atopic dermatitis (AD) were combined to clarify the effect of opportunistic Toxoplama gondii infection on the development of AD. AD was induced as a chronic contact hypersensitivity (CHS) with repeated challenge of 2,4,6-trinitro-1-chlorobenzene (TNCB) on the dorsal skin of mice. TNCB induced skin thickness increases in both normal and toxoplasmic mice. The changing patterns were different from the sigmoidal which saturated at 20 days in normal mice to the convex saturated at 12 days in toxoplasmic mice with the crossing at 18 days. Compared to normal mice, toxoplasmic mice presented CHS more severely in earlier times and then moderately in later times. These data suggest that host immune modification by T. gondii infection enhances CHS in early times of atopic stimulation but soothes the reaction of CHS in later times in mouse model.

Citations

Citations to this article as recorded by  Crossref logo
  • Effect of orally administered exopolysaccharides produced by Lactococcus lactis subsp. cremoris FC on a mouse model of dermatitis induced by repeated exposure to 2,4,6-trinitro-1-chlorobenzene
    Yayoi Gotoh, Shiho Suzuki, Midori Amako, Shinichi Kitamura, Toshiya Toda
    Journal of Functional Foods.2017; 35: 43.     CrossRef
  • 8,903 View
  • 86 Download
  • 1 Web of Science
  • Crossref
Toxoplasmosis in a Pet Peach-Faced Lovebird(Agapornis roseicollis)
Madalyn K. Cooper, Jan ?lapeta, Shannon L. Donahoe, David N. Phalen
Korean J Parasitol 2015;53(6):749-753.
Published online December 31, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.6.749
Toxoplasma gondii atypical type II genotype was diagnosed in a pet peach-faced lovebird (Agapornis roseicollis) based on histopathology, immunohistochemistry, and multilocus DNA typing. The bird presented with severe neurological signs, and hematology was suggestive of chronic granulomatous disease. Gross post-mortem examination revealed cerebral hemorrhage, splenomegaly, hepatitis, and thickening of the right ventricular free wall. Histologic sections of the most significant lesions in the brain revealed intralesional protozoan organisms associated with malacia, spongiform changes, and a mild histiocytic response, indicative of diffuse, non-suppurative encephalitis. Immunohistochemistry confirmed the causative organisms to be T. gondii. DNA isolated from the brain was used to confirm the presence of T. gondii DNA. Multilocus genotyping based on SAG1, altSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico markers demonstrated the presence of ToxoDB PCR-RFLP genotype #3 and B1 gene as atypical T. gondii type II. The atypical type II strain has been previously documented in Australian wildlife, indicating an environmental transmission route.

Citations

Citations to this article as recorded by  Crossref logo
  • Dolphins share Toxoplasma gondii Type II genotypes with terrestrial animals: Evidence of terrestrial T. gondii contamination in marine environments
    Yi-Jou Chen, Heather Fenton, Jane Hall, Karrie Rose, Victor M. Peddemors, Jan Šlapeta
    Veterinary Parasitology.2025; 335: 110439.     CrossRef
  • Global prevalence of Toxoplasma gondii in birds: A systematic review and meta-analysis
    Leila Zaki, Meysam Olfatifar, Fatemeh Ghaffarifar, Aida Vafae Eslahi, Amir KarimiPourSaryazdi, Ali Taghipour, Najla Hamidianfar, Milad Badri, Pikka Jokelainen
    Parasite Epidemiology and Control.2024; 25: e00350.     CrossRef
  • Successful Neoadjuvant Chemotherapy and Surgical Removal of a Nonmetastatic Testicular Round Cell Tumor in a Solomon Island Eclectus Parrot (Eclectus roratus solomonensis)
    Rachel Baden, Brian Speer, Michael Garner, Vanessa Hernández Urraca, Brenna Fitzgerald
    Journal of Avian Medicine and Surgery.2024;[Epub]     CrossRef
  • Epidemiology and isolation of viable Toxoplasma gondii strain from macropods
    Liulu Yang, Hongjie Ren, Niuping Zhu, Gaohui Mao, Junbao Li, Chunlei Su, Yibao Jiang, Yurong Yang
    Heliyon.2023; 9(3): e13960.     CrossRef
  • Epidemiologic significance of Toxoplasma gondii infections in turkeys, ducks, ratites and other wild birds: 2009–2020
    J. P. Dubey, F. H. A. Murata, C. K. Cerqueira-Cézar, O. C. H. Kwok, C. Su
    Parasitology.2021; 148(1): 1.     CrossRef
  • Comparison of genotypes of Toxoplasma gondii in domestic cats from Australia with latent infection or clinical toxoplasmosis
    Anthea Brennan, Shannon L. Donahoe, Julia A. Beatty, Katherine Belov, Scott Lindsay, Katherine A. Briscoe, Jan Šlapeta, Vanessa R. Barrs
    Veterinary Parasitology.2016; 228: 13.     CrossRef
  • 11,541 View
  • 113 Download
  • 8 Web of Science
  • Crossref

Original Article

Seropositivity of Toxoplasmosis in Pregnant Women by ELISA at Minia University Hospital, Egypt
Amany M. Kamal, Azza K. Ahmed, Manal Z. M. Abdellatif, Mohamed Tawfik, Ebtesam E. Hassan
Korean J Parasitol 2015;53(5):605-610.
Published online October 29, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.5.605
Toxoplasmosis is considered as an important risk factor for bad obstetric history (BOH) and one of the major causes of congenitally acquired infections. The present study aimed to estimate the seropositivity of T. gondii infection and associated risk factors among the attendees of high risk pregnancy and low risk antenatal care clinic of Minia Maternity and Pediatric University Hospital, Minia, Egypt. The study was carried out from April 2013 to April 2014 through 2 phases, the first phase was case-control study, and the second phase was follow-up with intervention. A total of 120 high risk pregnant and 120 normal pregnant females were submitted to clinical examinations, serological screening for anti-Toxoplasma IgM and IgG antibodies by ELISA, and an interview questionnaire. Seropositive cases were subjected to spiramycin course treatment. The results showed that the seroprevalence of toxoplasmosis in high-risk pregnancy group was 50.8%, which was significantly different from that of normal pregnancy group (P < 0.05). Analysis of seropositive women in relation to BOH showed that abortion was the commonest form of the pregnancy wastage (56.5%). The high prevalence of T. gondii seropositive cases was observed in the age group of 21-30 years. Post-delivery adverse outcome was observed in 80.3% of high-risk pregnancy group compared to 20% of normal pregnancy group. There was a statistically significant relationship between seropositivity and living in rural area, low socioeconomic level, and undercooked meat consumption (P<0.05). Serological screening for anti-Toxoplasma antibodies should be routine tests especially among high-risk pregnant women.

Citations

Citations to this article as recorded by  Crossref logo
  • Seroprevalence of Toxoplasma gondii Antibodies and Associated Risk Factors Among Women in Zakho City, Iraq
    Kalthom M Mustafa, Ahmed B Mohammed, Wijdan M.S. Mero
    Cureus.2024;[Epub]     CrossRef
  • Interplay between cross sectional analysis of risk factors associated with Toxoplasma gondii infection in pregnant women and their domestic cats
    Eman A. A. Hassanen, Dennis N. Makau, Mohamed Afifi, Omar A. Al-Jabr, Mohammed Abdulrahman Alshahrani, Ahmed Saif, Reham G. A. Anter, Wafaa M. El-Neshwy, Doaa Ibrahim, Rasha M. M. Abou Elez
    Frontiers in Veterinary Science.2023;[Epub]     CrossRef
  • Upregulation of IL-33, CCL2, and CXCL16 levels in Brazilian pregnant women infected by Toxoplasma gondii
    Priscilla Vilela dos Santos, Débora Nonato Miranda de Toledo, Nathalia Sernizon Guimarães, Luiza Oliveira Perucci, Valter Ferreira de Andrade-Neto, André Talvani
    Acta Tropica.2023; 243: 106931.     CrossRef
  • Latent Toxoplasmosis is Associated with Depression and Suicidal Behavior
    Ahmed M. Kamal, Amany M. Kamal, Aliaa S. Abd El-Fatah, Mina M. Rizk, Eptesam E. Hassan
    Archives of Suicide Research.2022; 26(2): 819.     CrossRef
  • Detection of toxoplasmosis in aborted women in Alexandria, Egypt using ELISA and PCR
    Mona Hassan El-Sayad, Aziza Ibrahim Salem, Hisham Fazary, Haneen Nawaf Alzainny, Naglaa Fathi Abd El-Latif
    Journal of Parasitic Diseases.2021; 45(2): 539.     CrossRef
  • Seroprevalence of Neospora caninum and Toxoplasma gondii IgG and IgM antibodies among buffaloes and cattle from Menoufia Province, Egypt
    Hany M. Ibrahim, Adel A. H. Abdel-Rahman, Nora M. Bishr
    Journal of Parasitic Diseases.2021; 45(4): 952.     CrossRef
  • Distribution of integrons and phylogenetic groups among Escherichia coli causing community-acquired urinary tract infection in Upper Egypt
    Eman M. Farahat, Noha A. Hassuna, Adel M. Hammad, Medhat Abdel Fattah, Ahmed S. Khairalla
    Canadian Journal of Microbiology.2021; 67(6): 451.     CrossRef
  • Evaluation of soluble CD279 (sCD279) and CD274 (sCD274) in Iraqi patients with Acute Myeloid Leukemia (AML) with Toxoplasmosis
    Ali A. Issa, Ali N. Yaseen, Muhammed A. H. Aldabagh
    IOP Conference Series: Earth and Environmental Science.2021; 790(1): 012065.     CrossRef
  • Toxoplasma gondii infection and spontaneous abortion: A systematic review and meta-analysis
    Narges Kalantari, Tahmineh Gorgani-Firouzjaee, Zahra Moulana, Mohammad Chehrazi, Salman Ghaffari
    Microbial Pathogenesis.2021; 158: 105070.     CrossRef
  • Toxoplasmosis in immunocompetent Saudi women: Correlation with vitamin D
    Zafar Rasheed, Ali Shariq, Ghaida B AlQefari, Ghayda S Alwahbi, Amal I Aljuaythin, Fidaa S Alsuhaibani, Daliyah F Alotaibi, Shahad S Aljohani, Ruqiah Alghasham, Thamir Alsaeed, Nada A Alharbi, Osamah Al Rugaie, Waleed Al Abdulmonem, Osama F Sharaf
    Women's Health.2021;[Epub]     CrossRef
  • Toxoplasma gondii seropositivity and the associated risk factors in sheep and pregnant women in El-Minya Governorate, Egypt
    Abdelbaset E. Abdelbaset, Maha I. Hamed, Mostafa F. N. Abushahba, Mohamed S. Rawy, Amal S. M. Sayed, Jeffrey J. Adamovicz
    Veterinary World.2020; 13(1): 54.     CrossRef
  • A review on toxoplasmosis in humans and animals from Egypt
    I. E. Abbas, I. Villena, J. P. Dubey
    Parasitology.2020; 147(2): 135.     CrossRef
  • The global seroprevalence of anti-Toxoplasma gondii antibodies in women who had spontaneous abortion: A systematic review and meta-analysis
    Tooran Nayeri, Shahabeddin Sarvi, Mahmood Moosazadeh, Afsaneh Amouei, Zahra Hosseininejad, Ahmad Daryani, Maria Angeles Gómez-Morales
    PLOS Neglected Tropical Diseases.2020; 14(3): e0008103.     CrossRef
  • Risk factors associated with seropositivity for Toxoplasma gondii in population-based studies among immunocompromised patients (pregnant women, HIV patients and children) in West African countries, Cameroon and Gabon: a meta-analysis
    Paul Olalekan Odeniran, Kehinde Foluke Omolabi, Isaiah Oluwafemi Ademola
    Acta Tropica.2020; 209: 105544.     CrossRef
  • Serological prevalence of toxoplasmosis in pregnant women in Luanda (Angola): Geospatial distribution and its association with socio-demographic and clinical-obstetric determinants
    Amélia Nkutxi Vueba, Clarissa Perez Faria, Ricardo Almendra, Paula Santana, Maria do Céu Sousa, Adriana Calderaro
    PLOS ONE.2020; 15(11): e0241908.     CrossRef
  • Clinico-epidemiological and sociodemographic profile of HIV/AIDS patients who are co-infected with Toxoplasma gondii in the border region of Brazil
    JANIELLE C. MORO, NEIDE M. MOREIRA
    Anais da Academia Brasileira de Ciências.2020;[Epub]     CrossRef
  • Potential risk factors associated with seropositivity for Toxoplasma gondii among pregnant women and HIV infected individuals in Ethiopia: A systematic review and meta-analysis
    Zewdu Seyoum Tarekegn, Haileyesus Dejene, Agerie Addisu, Shimelis Dagnachew, Olaf Horstick
    PLOS Neglected Tropical Diseases.2020; 14(12): e0008944.     CrossRef
  • Toxoplasma gondii infection and toxoplasmosis in North Africa: a review
    Mariem Rouatbi, Safa Amairia, Yosra Amdouni, Mohamed Anis Boussaadoun, Ouarda Ayadi, Amira Adel Taha Al-Hosary, Mourad Rekik, Rym Ben Abdallah, Karim Aoun, Mohamed Aziz Darghouth, Barbara Wieland, Mohamed Gharbi
    Parasite.2019; 26: 6.     CrossRef
  • BCG scar, socioeconomic and nutritional status: a study of newborns in urban area of Makassar, Indonesia
    Aldian Irma Amaruddin, Sitti Wahyuni, Firdaus Hamid, Maisuri T. Chalid, Maria Yazdanbakhsh, Erliyani Sartono
    Tropical Medicine & International Health.2019; 24(6): 736.     CrossRef
  • Conhecimento e comportamento preventivo de gestantes sobre Toxoplasmose no município de Imperatriz, Maranhão, Brasil
    Ivone Pereira da Silva Moura, Ilma Pastana Ferreira, Altem Nascimento Pontes, Cléa Nazaré Carneiro Bichara
    Ciência & Saúde Coletiva.2019; 24(10): 3933.     CrossRef
  • Performance of Zika Assays in the Context of Toxoplasma gondii, Parvovirus B19, Rubella Virus, and Cytomegalovirus (TORCH) Diagnostic Assays
    Bettie Voordouw, Barry Rockx, Thomas Jaenisch, Pieter Fraaij, Philippe Mayaud, Ann Vossen, Marion Koopmans
    Clinical Microbiology Reviews.2019;[Epub]     CrossRef
  • Seroprevalence of Toxoplasma gondii among pregnant women attending antenatal clinics at Hawassa University comprehensive specialized and Yirgalem General Hospitals, in Southern Ethiopia
    Demissie Assegu Fenta
    BMC Infectious Diseases.2019;[Epub]     CrossRef
  • Toxoplasma gondii seroprevalence among pregnant women attending antenatal clinic in Northern Tanzania
    Eliakimu Paul, Ireen Kiwelu, Blandina Mmbaga, Rebeka Nazareth, Elias Sabuni, Athanasia Maro, Arnold Ndaro, Jo E. B. Halliday, Jaffu Chilongola
    Tropical Medicine and Health.2018;[Epub]     CrossRef
  • Seroprevalence and associated risk factors of Toxoplasma gondii among Manchu pregnant women in northeastern China
    Ruo-Lan Jiang, Ling-Hui Ma, Zhen-Rong Ma, Guangyu Hou, Quan Zhao, Xiang Wu
    Microbial Pathogenesis.2018; 123: 398.     CrossRef
  • Seroprevalence and potential risk factors of T. gondii infection in pregnant women attending antenatal care at Bonga Hospital, Southwestern Ethiopia
    Jalel Negero, Moti Yohannes, Kifle Woldemichael, Dechassa Tegegne
    International Journal of Infectious Diseases.2017; 57: 44.     CrossRef
  • Comparative and analytical study on active toxoplasmosis to assess the IgG avidity in correlation to serological profile in a cohort of Egyptian patients
    Al Shaimaa M. R. Hamed, Shadia H. Omar, Maha M. A. Basyoni, Abeer S. El Antably, Engy El Khateeb, Abdulla Y. El Kateb
    Comparative Clinical Pathology.2017; 26(5): 1157.     CrossRef
  • Molecular and serological prevalence of Toxoplasma gondii in pregnant women and sheep in Egypt
    Hany M. Ibrahim, Azza H. Mohamed, Ahmed A. El-Sharaawy, Hend E. El-Shqanqery
    Asian Pacific Journal of Tropical Medicine.2017; 10(10): 996.     CrossRef
  • Seroprevalence of Anti-Toxoplasma gondiiAntibodies Among Lebanese Pregnant Women
    Hasan Nahouli, Nour El Arnaout, Elias Chalhoub, Elie Anastadiadis, Hiba El Hajj
    Vector-Borne and Zoonotic Diseases.2017; 17(12): 785.     CrossRef
  • Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran
    Somaie Matin, Gholamreza Shahbazi, Shervin Tabrizian Namin, Rouhallah Moradpour, Farideh Feizi, Hadi Piri-dogahe
    The Korean Journal of Parasitology.2017; 55(6): 607.     CrossRef
  • Is Toxoplasma Gondii Infection a Risk Factor for Leukemia? An Evidence-Based Meta-Analysis
    Yi Huang, Yu Huang, Aoshuang Chang, Jishi Wang, Xiaoqing Zeng, Jiahong Wu
    Medical Science Monitor.2016; 22: 1547.     CrossRef
  • 13,788 View
  • 155 Download
  • 33 Web of Science
  • Crossref

Brief Communications

Prevalence of Toxoplasma gondii in Dogs in Zhanjiang, Southern China
Hai-Hai Jiang, Ming-Wei Li, Min-Jun Xu, Wei Cong, Xing-Quan Zhu
Korean J Parasitol 2015;53(4):493-496.
Published online August 25, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.4.493
Toxoplasmosis, caused by Toxoplasma gondii, is a parasitic zoonosis with worldwide distribution. The present study investigated the prevalence of T. gondii in dogs in Zhanjiang city, southern China, using both serological and molecular detection. A total of 364 serum samples and 432 liver tissue samples were collected from the slaughter house between December 2012 and January 2013 and were examined for T. gondii IgG antibody by ELISA and T. gondii DNA by semi-nested PCR based on B1 gene, respectively. The overall seroprevalence of T. gondii IgG antibody was 51.9%, and T. gondii DNA was detected in 37 of 432 (8.6%) liver tissue samples. These positive DNA samples were analyzed by PCR-RFLP at 3'- and 5'-SAG2. Only 8 samples gave the PCR-RFLP data, and they were all classified as type I, which may suggest that the T. gondii isolates from dogs in Zhanjiang city may represent type I or type I variant. This study revealed the high prevalence of T. gondii infection in dogs in Zhanjiang city, southern China. Integrated measures should be taken to prevent and control toxoplasmosis in dogs in this area for public health concern.

Citations

Citations to this article as recorded by  Crossref logo
  • An investigation into Toxoplasma gondii at the human-livestock-wildlife interface, South Africa
    Refilwe P. Bokaba, Veronique Dermauw, Darshana Morar-Leather, Pierre Dorny, Louis van Schalkwyk, Luis Neves
    Onderstepoort Journal of Veterinary Research.2024;[Epub]     CrossRef
  • Seropositivity of toxoplasmosis in pregnant women living with HIV/AIDS worldwide: A systematic review and meta-analysis
    Eden Woldegerima, Mulugeta Aemiro, Getnet Fetene, Nega Birhanie
    Parasitology International.2024; 102: 102922.     CrossRef
  • Exploring similarities and differences between Toxoplasma gondii and Neospora caninum infections in dogs
    Giulia Morganti, Giulia Rigamonti, Leonardo Brustenga, Valentina Calgaro, Giovanni Angeli, Iolanda Moretta, Manuela Diaferia, Fabrizia Veronesi
    Veterinary Research Communications.2024; 48(6): 3563.     CrossRef
  • Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea
    Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho
    The Korean Journal of Parasitology.2020; 58(3): 257.     CrossRef
  • Toxoplasma gondii infections in dogs: 2009-2020
    Jitender P. Dubey, Fernando H.A. Murata, Camila K. Cerqueira-Cézar, Oliver C.H. Kwok, Yurong Yang, Chunlei Su
    Veterinary Parasitology.2020; 287: 109223.     CrossRef
  • Toxoplasma gondii Triggers Neutrophil Extracellular Traps Release in Dogs
    Zhengkai Wei, Zedong Wang, Xiao Liu, Chaoqun Wang, Zhen Han, Di Wu, Yong Zhang, Xichen Zhang, Zhengtao Yang, Quan Liu
    Frontiers in Cellular and Infection Microbiology.2020;[Epub]     CrossRef
  • Prevalence of Toxoplasma gondii Antibodies in Stray Dogs from Various Locations in West and East Malaysia
    Malaika Watanabe, Mohammed Babatunde Sadiq, Nazrul Iqbal Abdul Mulop, Konto Mohammed, Puteri Azaziah Megat Rani, Lau Seng Fong, Nor Azlina Aziz, Juriah Kamaludeen, Siti Zubaidah Ramanoon, Rozaihan Mansor, Tan Li Ping, Sharifah Salmah Syed-Hussain
    The Korean Journal of Parasitology.2020; 58(5): 487.     CrossRef
  • Prevalence, risk factors and genetic characterization of Toxoplasma gondii in sick pigs and stray cats in Jiangsu Province, eastern China
    Zhao-feng Hou, Shi-jie Su, Dan-dan Liu, Le-le Wang, Chuan-li Jia, Zhen-xing Zhao, Yi-fei Ma, Qiao-qiao Li, Jin-jun Xu, Jian-ping Tao
    Infection, Genetics and Evolution.2018; 60: 17.     CrossRef
  • Prevalence and genetic characterization of Toxoplasma gondii in badgers ( Melogale moschata ) in southern China by PCR-RFLP
    Kai Chen, Si-Yang Huang, Jin-Lei Wang, Rong-Liang Hu, Qiu-Xia Yao, Shou-Feng Zhang, Xing-Quan Zhu, Quan Liu
    Infection, Genetics and Evolution.2017; 52: 30.     CrossRef
  • 10,080 View
  • 100 Download
  • 10 Web of Science
  • Crossref
Seroprevalence of Toxoplasma gondii Among Primary School Children in Shandong Province, China
Ke-Sheng Xin, Hui Liu, Hong-Bing Wang, Zong-Liang Yao
Korean J Parasitol 2015;53(4):489-492.
Published online August 25, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.4.489
Although Toxoplasma gondii infection in primary school children has been investigated in many countries, limited surveys have been available in primary school children in China. In the present study, we report the seroprevalence of T. gondii infection in primary school children in Shandong province, China. Sera from 6,000 primary school children were evaluated for T. gondii antibodies with ELISA. The overall seroprevalence of T. gondii infection was 16.0% (961/6,000), of which 14.5% (870/6,000) were positive for anti-T. gondii IgG antibodies, 3.4% (206/6,000) positive for IgM, and 1.9% (115/6,000) were positive for both IgG and IgM. The results of the present investigation indicated a high seroprevalence of T. gondii infection in primary school children in Shandong province, China. Therefore, effective measures should be taken to prevent and control T. gondii infection in primary school children in this province. To the best of our knowledge, this is the first report of T. gondii seroprevalence in primary school children in Shandong province, China.

Citations

Citations to this article as recorded by  Crossref logo
  • Seroprevalence of Toxoplasma gondii infection among patients of a tertiary hospital in Guangzhou, Guangdong province, PR China
    Yu-bin Guan, Xiao-xiao Sun, Shao-lian Chen, Xiao-ting Zhu, Zhi-hua Zeng, Han-wei Lu, Hong-mei Feng, Yu Guo, Wen-gong Jiang, Kui Xiong, Xiao-rong Yang, Ho-Woo Nam, Zhao-shou Yang, Pan Li
    PLOS ONE.2023; 18(7): e0286430.     CrossRef
  • Seroprevalence of toxoplasmosis at referral hospitals in the northwestern region, Saudi Arabia
    Talal Alharazi, Jerold C. Alcantara, Lea L. Dando, Petelyne Pangket
    Journal of Education and Health Promotion.2023;[Epub]     CrossRef
  • Seroprevalence and Molecular Investigation of Toxoplasmosis Among Working Children in Tehran
    Farah Bokharaei-Salim, Khadijeh Khanaliha, Shirin Sayyahfar, Mohsen Sadeghi, Ahmad Tavakoli, Borna Salemi
    Archives of Pediatric Infectious Diseases.2022;[Epub]     CrossRef
  • Seroprevalence and risk factors of Toxoplasma gondii infection in primary school children in Henan province, central China
    Shuai Wang, Zhijun Yao, Haoran Li, Pengju Li, Dong Wang, Haizhu Zhang, Qing Xie, Zhenchao Zhang, Xiangrui Li
    Parasite.2020; 27: 23.     CrossRef
  • A Large-Scale Serological Survey of Toxoplasma gondii Infection Among Persons Participated in Health Screening in Yunnan Province, Southwestern China
    Zhao Li, Chun-Yi Sun, Ren-Dan Yang, Chang-Jun Xu, Mei-Chen Chen, Yun-Jiang Zhang, Wei Cong, Xing-Quan Zhu, Yang Liu, Feng-Cai Zou
    Vector-Borne and Zoonotic Diseases.2019; 19(6): 441.     CrossRef
  • Seroprevalence of Toxoplasma gondii among School Children in Pyin Oo Lwin and Naung Cho, Upper Myanmar
    Thị Lam Thái, Hojong Jun, Seo-Hye Park, Hương Giang Lê, Jinyoung Lee, Seong Kyu Ahn, Jung-Mi Kang, Moe Kyaw Myint, Khin Lin, Woon-Mok Sohn, Ho-Woo Nam, Byoung-Kuk Na, Tong-Soo Kim
    The Korean Journal of Parasitology.2019; 57(3): 303.     CrossRef
  • Toxoplasma gondii: seroprevalence and associated risk factors among preschool-aged children in Osun State, Nigeria
    Oluyomi A Sowemimo, Tsung-Han Wu, Yueh-Lun Lee, Samuel O Asaolu, Ting-Wu Chuang, Olaoluwa P Akinwale, Bolaji O Badejoko, Vincent P Gyang, Timothy Nwafor, Emmanuel Henry, Chia-Kwung Fan
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2018;[Epub]     CrossRef
  • Toxoplasma gondii Exposure and the Risk of Attention Deficit Hyperactivity Disorder in Children and Adolescents
    Shahram Khademvatan, Forough Riahi, Maryam Izadi-Mazidi, Nilufar Khajeddin, Elham Yousefi
    Pediatric Infectious Disease Journal.2018; 37(11): 1097.     CrossRef
  • Seroprevalence and associated risk factors ofToxoplasma gondiiinfection in the Korean, Manchu, Mongol and Han ethnic groups in eastern and northeastern China
    X.-X. ZHANG, Q. ZHAO, C.-W. SHI, W.-T. YANG, Y.-L. JIANG, Z.-T. WEI, C.-F. WANG, G.-L. YANG
    Epidemiology and Infection.2016; 144(9): 2018.     CrossRef
  • 10,701 View
  • 91 Download
  • 11 Web of Science
  • Crossref
Genetic Diversity of Toxoplasma gondii Strains from Different Hosts and Geographical Regions by Sequence Analysis of GRA20 Gene
Hong-Rui Ning, Si-Yang Huang, Jin-Lei Wang, Qian-Ming Xu, Xing-Quan Zhu
Korean J Parasitol 2015;53(3):345-348.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.345
Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.

Citations

Citations to this article as recorded by  Crossref logo
  • Effects of latent infection of Toxoplasma gondii strains with different genotypes on mouse behavior and brain transcripts
    Bei-Bei Zhou, Hong-Jie Dong, Hang Sun, Xiao-Man Xie, Huan-Huan Xie, Wen-Ju Zhu, Ya-Nan Li, Chao Xu, Jian-Ping Cao, Gui-Hua Zhao, Kun Yin
    Parasites & Vectors.2025;[Epub]     CrossRef
  • First Report on the Molecular Detection and Genetic Characterization of Toxoplasma gondii From Donkeys in Kenya
    Fredrick O. Obonyo, Ndichu Maingi, Samuel M. Githigia, Kevin O. Ochwedo, Anne A. Owiti, Evans N. Nyaboga
    Acta Parasitologica.2024; 69(3): 1480.     CrossRef
  • 9,424 View
  • 101 Download
  • 2 Web of Science
  • Crossref
Sequence Diversity in MIC6 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations
Zhong-Yuan Li, Hui-Qun Song, Jia Chen, Xing-Quan Zhu
Korean J Parasitol 2015;53(3):341-344.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.341
Toxoplasma gondii is an opportunistic protozoan parasite that can infect almost all warm-blooded animals including humans with a worldwide distribution. Micronemes play an important role in invasion process of T. gondii, associated with the attachment, motility, and host cell recognition. In this research, sequence diversity in microneme protein 6 (MIC6) gene among 16 T. gondii isolates from different hosts and geographical regions and 1 reference strain was examined. The results showed that the sequence of all the examined T. gondii strains was 1,050 bp in length, and their A + T content was between 45.7% and 46.1%. Sequence analysis presented 33 nucleotide mutation positions (0-1.1%), resulting in 23 amino acid substitutions (0-2.3%) aligned with T. gondii RH strain. Moreover, T. gondii strains representing the 3 classical genotypes (Type I, II, and III) were separated into different clusters based on the locus of MIC6 using phylogenetic analyses by Bayesian inference (BI), maximum parsimony (MP), and maximum likelihood (ML), but T. gondii strains belonging to ToxoDB #9 were separated into different clusters. Our results suggested that MIC6 gene is not a suitable marker for T. gondii population genetic studies.
  • 9,980 View
  • 93 Download
  • 1 Web of Science

Original Articles

Toxoplasma gondii B1 Gene Detection in Feces of Stray Cats around Seoul, Korea and Genotype Analysis of Two Laboratory-Passaged Isolates
Bong-Kwang Jung, Sang-Eun Lee, Hyemi Lim, Jaeeun Cho, Deok-Gyu Kim, Hyemi Song, Min-Jae Kim, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2015;53(3):259-263.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.259
The increasing prevalence of Toxoplasma gondii infection in the human population in the Republic of Korea (= Korea) is due to various reasons such as an increase in meat consumption. However, the importance of cats in transmitting T. gondii infection through oocysts to humans has seldom been assessed. A total of 300 fecal samples of stray cats captured around Seoul from June to August 2013 were examined for T. gondii B1 gene (indicating the presence of oocysts) using nested-PCR. Fourteen (4.7%) of 300 cats examined were positive for B1 gene. Female cats (7.5%) showed a higher prevalence than male cats (1.4%). Cats younger than 3 months (5.5%) showed a higher prevalence than cats (1.5%) older than 3 months. For laboratory passage of the positive samples, the fecal suspension (0.2 ml) of B1 gene positive cats was orally inoculated into experimental mice. Brain tissues of the mice were obtained after 40 days and examined for the presence of tissue cysts. Two isolates were successfully passaged (designated KNIH-1 and KNIH-2) and were molecularly analyzed using the SAG5D and SAG5E gene sequences. The SAG5D and SAG5E gene sequences showed high homologies with the ME49 strain (less virulent strain). The results indicated the importance of stray cats in transmitting T. gondii to humans in Korea, as revealed by detection of B1 gene in fecal samples. T. gondii isolates from cats were successfully passaged in the laboratory for the first time in Korea.

Citations

Citations to this article as recorded by  Crossref logo
  • Molecular detection of Toxoplasma gondii in wild oysters and seawater on the southeast coast of South Korea
    Hyejoo Shin, Sooji Hong, Seungwan Ryoo, Jong-Yil Chai, Bong-Kwang Jung
    Food Microbiology.2026; 134: 104925.     CrossRef
  • Molecular detection and genotyping of Toxoplasma gondii in stray cat feces from Khorramabad, West Iran
    Hakim Azizi, Maryam Hataminejad, Ali Taghipour, Maryam Norouzi, Aliyar Mirzapour
    Veterinary and Animal Science.2024; 25: 100389.     CrossRef
  • Association between domestic animal exposure and diarrhea prevalence in under- five children in low- and middle-income countries: a systematic review and meta-analysis
    Atalay Getachew, Mesafint Molla, Amha Admasie, Muluken Azage Yenesew
    BMC Pediatrics.2024;[Epub]     CrossRef
  • Prevalence of Toxoplasma gondii‐like oocyst shedding in feral and owned cats in Damascus, Syria
    Mohammad Taher Ismail, Abeer Al‐Kafri
    Journal of Veterinary Internal Medicine.2023; 37(3): 976.     CrossRef
  • Molecular Characterization of Toxoplasma gondii in Cats and Its Zoonotic Potential for Public Health Significance
    Mian Abdul Hafeez, Muntazir Mehdi, Faiza Aslam, Kamran Ashraf, Muhammad Tahir Aleem, Abdur Rauf Khalid, Adeel Sattar, Syeda Fakhra Waheed, Abdulaziz Alouffi, Omar Obaid Alharbi, Muhammad Abu Bakr Shabbir, Umer Chaudhry, Mashal M. Almutairi
    Pathogens.2022; 11(4): 437.     CrossRef
  • Prevalence and Molecular Characterization of Toxoplasma gondii and Toxocara cati Among Stray and Household Cats and Cat Owners in Tehran, Iran
    Poorya Karimi, Soheila Shafaghi-Sisi, Ahmad Reza Meamar, Gelareh Nasiri, Elham Razmjou
    Frontiers in Veterinary Science.2022;[Epub]     CrossRef
  • Molecular detection and dense granule antigen 6 genotyping of feline Toxoplasma gondii in Phayao, Thailand
    Chittakun Suwancharoen, Chorpaka Phuangsri, Khanuengnij Prakhammin, Ornampai Japa
    Veterinary World.2022; : 2309.     CrossRef
  • DNA-based detection of Leptospira wolffii, Giardia intestinalis and Toxoplasma gondii in environmental feces of wild animals in Korea
    Priyanka KUMARI, Kyung Yeon EO, Woo-Shin LEE, Junpei KIMURA, Naomichi YAMAMOTO
    Journal of Veterinary Medical Science.2021; 83(5): 850.     CrossRef
  • Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia
    Alejandro Zamora-Vélez, Jessica Triviño, Sebastián Cuadrado-Ríos, Fabiana Lora-Suarez, Jorge Enrique Gómez-Marín
    Parasite.2020; 27: 25.     CrossRef
  • All about toxoplasmosis in cats: the last decade
    J.P. Dubey, C.K. Cerqueira-Cézar, F.H.A. Murata, O.C.H. Kwok, Y.R. Yang, C. Su
    Veterinary Parasitology.2020; 283: 109145.     CrossRef
  • Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea
    Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho
    The Korean Journal of Parasitology.2020; 58(3): 257.     CrossRef
  • Molecular Detection and Genetic Diversity of Toxoplasma gondii Oocysts in Cat Faeces from Klang Valley, Malaysia, Using B1 and REP Genes in 2018
    Mohammed Nasiru Wana, Mohamad Aris Mohd Moklas, Malaika Watanabe, Ngah Zasmy Unyah, Sharif Alhassan Abdullahi, Ashraf Ahmad Issa Alapid, Norshariza Nordin, Rusliza Basir, Roslaini Abd Majid
    Pathogens.2020; 9(7): 576.     CrossRef
  • Genetic Analysis of Zoonotic Gastrointestinal Protozoa and Microsporidia in Shelter Cats in South Korea
    Dongmi Kwak, Min-Goo Seo
    Pathogens.2020; 9(11): 894.     CrossRef
  • Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran
    Majid Khodaverdi, Gholamreza Razmi
    BMC Veterinary Research.2019;[Epub]     CrossRef
  • An outbreak of toxoplasmosis in squirrel monkeys (Saimiri sciureus) in South Korea
    Hanseul Oh, Kyung‐Yeon Eo, Sanjeev Gumber, Jung Joo Hong, C‐Yoon Kim, Hyun‐Ho Lee, Young‐Mok Jung, Jin Kim, Gyu‐Whan Whang, Ji‐Min Lee, Yong‐Gu Yeo, Bokyeong Ryu, Ji‐Sook Ryu, Seul‐Kee Lee, Ukjin Kim, Sin‐Geun Kang, Jae‐Hak Park
    Journal of Medical Primatology.2018; 47(4): 238.     CrossRef
  • Seroprevalence and Risk Factors of Toxoplasma gondii Infection among Cat Sitters in Korea
    Bong-Kwang Jung, Hyemi Song, Sang-Eun Lee, Min-Jae Kim, Jaeeun Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2017; 55(2): 203.     CrossRef
  • Toxoplasma genotyping in congenital toxoplasmosis in Upper Egypt: evidence of type I strain
    Hanan E. M. Eldeek, Alzahraa Abdel Raouf Ahmad, Mohamed Ahmed El-Mokhtar, Abdel Rahman M.M. Abdel Kader, Ahmad M. Mandour, Mahmoud Elhady M. Mounib
    Parasitology Research.2017; 116(9): 2393.     CrossRef
  • Exposure to Animal Feces and Human Health: A Systematic Review and Proposed Research Priorities
    Gauthami Penakalapati, Jenna Swarthout, Miranda J. Delahoy, Lydia McAliley, Breanna Wodnik, Karen Levy, Matthew C. Freeman
    Environmental Science & Technology.2017; 51(20): 11537.     CrossRef
  • Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP
    Wen-Bin Zheng, Xiao-Xuan Zhang, Jian-Gang Ma, Fa-Cai Li, Quan Zhao, Si-Yang Huang, Xing-Quan Zhu, Gordon Langsley
    PLOS ONE.2016; 11(11): e0165308.     CrossRef
  • 9,955 View
  • 116 Download
  • 22 Web of Science
  • Crossref
Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions
Shuai Wang, Aiping Cao, Xun Li, Qunli Zhao, Yuan Liu, Hua Cong, Shenyi He, Huaiyu Zhou
Korean J Parasitol 2015;53(3):253-258.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.253
Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.

Citations

Citations to this article as recorded by  Crossref logo
  • Superoxide Dismutases in Immune Regulation and Infectious Diseases
    Tong Liu, Jiajin Shang, Qijun Chen
    Antioxidants.2025; 14(7): 809.     CrossRef
  • Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase (TgSOD) induces partial immune protection against acute toxoplasmosis in BALB/c mice
    Yuan Liu, Aiping Cao, Yawen Li, Xun Li, Hua Cong, Shenyi He, Huaiyu Zhou
    BMC Infectious Diseases.2017;[Epub]     CrossRef
  • 10,317 View
  • 102 Download
  • 2 Web of Science
  • Crossref
Pathogenicity of Five Strains of Toxoplasma gondii from Different Animals to Chickens
Shuai Wang, Guang-Wei Zhao, Wang Wang, Zhen-Chao Zhang, Bo Shen, I. A. Hassan, Qing Xie, Ruo-Feng Yan, Xiao-Kai Song, Li-Xin Xu, Xiang-Rui Li
Korean J Parasitol 2015;53(2):155-162.
Published online April 22, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.2.155
Toxoplasma gondii is a protozoan parasite with a broad range of intermediate hosts. Chickens as important food-producing animals can also serve as intermediate hosts. To date, experimental studies on the pathogenicity of T. gondii in broiler chickens were rarely reported. The
objective
of the present study was to compare the pathogenicity of 5 different T. gondii strains (RH, CN, JS, CAT2, and CAT3) from various host species origin in 10-day-old chickens. Each group of chickens was infected intraperitoneally with 5×108, 1×108, 1×107, and 1×106 tachyzoites of the 5 strains, respectively. The negative control group was mockly inoculated with PBS alone. After infection, clinical symptoms and rectal temperatures of all the chickens were checked daily. Dead chickens during acute phage of the infection were checked for T. gondii tachyzoites by microscope, while living cases were checked for T. gondii infection at day 53 post-inoculation (PI) by PCR method. Histopathological sections were used to observe the pathological changes in the dead chickens and the living animals at day 53 PI. No significant differences were found in survival periods, histopathological findings, and clinical symptoms among the chickens infected with the RH, CN, CAT2, and CAT3 strains. Histopathological findings and clinical symptoms of the JS (chicken origin) group were similar to the others. However, average survival times of infected chickens of the JS group inoculated with 5×108 and 1×108 tachyzoites were 30.0 and 188.4 hr, respectively, significantly shorter than those of the other 4 mammalian isolates. Chickens exposed to 108 of T. gondii tachyzoites and higher showed acute signs of toxoplasmosis, and the lesions were relatively more severe than those exposed to lower doses. The results indicated that the pathogenicity of JS strain was comparatively stronger to the chicken, and the pathogenicity was dose-dependent.

Citations

Citations to this article as recorded by  Crossref logo
  • Application of gold immunochromatographic assay strip combined with digital evaluation for early detection of Toxoplasma gondii infection in multiple species
    Jiyuan Fan, Hao Sun, Jiawen Fang, Yafan Gao, Haojie Ding, Bin Zheng, Qingming Kong, Xunhui Zhuo, Shaohong Lu
    Parasites & Vectors.2024;[Epub]     CrossRef
  • First identified Toxoplasma gondii Type I in market-sold ducks in Fujian province, China: a significant for public health
    Si-Ang Li, Li-Yuan Huang, Xu-Dong Guo, Wen-Yuan Miao, Ying-Sheng Lin, Dong-Hui Zhou
    Poultry Science.2024; 103(9): 104024.     CrossRef
  • Epidemiological investigation and reinfection evaluation of Toxoplasma gondii in chickens in Shandong Province, China
    Xiao Chen, Pei Sun, Junpeng Chen, Qianqian Tan, Jinxuan Li, Xiaomei Liu, Qianqian Xiao, Hongmei Li, Xiaomin Zhao, Ningning Zhao, Xiao Zhang
    Experimental Parasitology.2022; 238: 108276.     CrossRef
  • Experimental infection of Toxoplasma gondii in specific pathogen-free and commercial broiler chicks
    Xiao Chen, Junpeng Chen, Qianqian Tan, Jinxuan Li, Xiaomei Liu, Qianqian Xiao, Hongmei Li, Xiaomin Zhao, Xiao Zhang
    Comparative Immunology, Microbiology and Infectious Diseases.2022; 90-91: 101890.     CrossRef
  • Application of Toxoplasma gondii-specific SAG1, GRA7 and BAG1 proteins in serodiagnosis of animal toxoplasmosis
    Tongsheng Qi, Jingkai Ai, Yali Sun, Hejia Ma, Ming Kang, Xiaoqian You, Jixu Li
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade
    J. P. Dubey, H. F. J. Pena, C. K. Cerqueira-Cézar, F. H. A. Murata, O. C. H. Kwok, Y. R. Yang, S. M. Gennari, C. Su
    Parasitology.2020; 147(12): 1263.     CrossRef
  • Toxoplasma gondii infection and toxoplasmosis in farm animals: Risk factors and economic impact
    S. Stelzer, W. Basso, J. Benavides Silván, L.M. Ortega-Mora, P. Maksimov, J. Gethmann, F.J. Conraths, G. Schares
    Food and Waterborne Parasitology.2019; 15: e00037.     CrossRef
  • Characterisation of susceptibility of chicken macrophages to infection with Toxoplasma gondii of type II and III strains
    Irene Malkwitz, Angela Berndt, Arwid Daugschies, Berit Bangoura
    Experimental Parasitology.2018; 187: 22.     CrossRef
  • Chicken line-dependent mortality after experimental infection with three type IIxIII recombinant Toxoplasma gondii clones
    G. Schares, D.C. Herrmann, P. Maksimov, B. Matzkeit, F.J. Conraths, G. Moré, R. Preisinger, S. Weigend
    Experimental Parasitology.2017; 180: 101.     CrossRef
  • Diagnosis of Toxoplasmosis Using Affinity Purified Fraction of Tachyzoites Local Isolate
    Soad E. Hassan, Nagwa I. Toaleb, Raafat M. Shaapan, Eman Hussien Abdel-Rahm, Ehab Kotb Elmahallaw
    Research Journal of Parasitology.2016; 11(1): 13.     CrossRef
  • Seroprevalence of Toxoplasma gondii infection among patients with hand, foot and mouth disease in Henan, China: a hospital-based study
    Shuai Wang, Chunwei Lan, Luwen Zhang, Haizhu Zhang, Zhijun Yao, Dong Wang, Jingbo Ma, Jiarong Deng, Shiguo Liu
    Infectious Diseases of Poverty.2015;[Epub]     CrossRef
  • 10,717 View
  • 119 Download
  • 11 Web of Science
  • Crossref
Seropositivity and Serointensity of Toxoplasma gondii Antibodies and DNA among Patients with Schizophrenia
Ainsah Omar, Osman Che Bakar, Nor Fatini Adam, Hakim Osman, Arina Osman, Ahmad Hatim Suleiman, Mohd Rizal Abdul Manaf, Mohd Ikhsan Selamat
Korean J Parasitol 2015;53(1):29-34.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.29

The aim of this cross sectional case control study was to examine the serofrequency and serointensity of Toxoplasma gondii (Tg) IgG, IgM, and DNA among patients with schizophrenia. A total of 101 patients with schizophrenia and 55 healthy controls from Sungai Buloh Hospital, Selangor, Malaysia and University Malaya Medical Center (UMMC) were included in this study. The diagnosis of schizophrenia was made based on the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV). The presence of Tg infection was examined using both indirect (ELISA) and direct (quantitative real-time PCR) detection methods by measuring Tg IgG and IgM and DNA, respectively. The serofrequency of Tg IgG antibodies (51.5%, 52/101) and DNA (32.67%, 33/101) among patients with schizophrenia was significantly higher than IgG (18.2%, 10/55) and DNA (3.64%, 2/55) of the controls (IgG, P=0.000, OD=4.8, CI=2.2-10.5; DNA, P=0.000, OD=12.9, CI=2.17-10.51). However, the Tg IgM antibody between patients with schizophrenia and controls was not significant (P>0.005). There was no significant difference (P>0.005) in both serointensity of Tg IgG and DNA between patients with schizophrenia and controls. These findings have further demonstrated the strong association between the active Tg infection and schizophrenia.

Citations

Citations to this article as recorded by  Crossref logo
  • Reconsidering Toxoplasmosis Prevention and Treatment Due to Its Relation to Neuropsychiatric Disturbances
    Fabrizio Bruschi, Silvia Fabiani
    Zoonotic Diseases.2025; 5(2): 8.     CrossRef
  • Seroprevalence and Associated Risk Factors of Toxoplasma gondii in Patients Diagnosed with Schizophrenia: A Case–Control Cross Sectional Study
    Sebastian Grada, Alin Gabriel Mihu, Daniela Adriana Oatis, Constantin Catalin Marc, Liana Maria Chicea, Cristina Petrescu, Alina Maria Lupu, Tudor Rares Olariu
    Biomedicines.2024; 12(5): 998.     CrossRef
  • Seroprevalence and sociodemographic characteristics of Toxoplasma gondii infection in patients with psychiatric disorders in Malaysia
    Alia Maisarah, Suharni Mohamad, Maruzairi Husain, Sarimah Abdullah, Rahmah Noordin
    Acta Tropica.2024; 255: 107241.     CrossRef
  • The Spread of Toxoplasmosis in Women within Al-Rifai District
    Rehab Issa Hashem Suhail, Shimaa Ahmed Mutab Nayef, Teeba Sabah Daham Mohammed, Yaqeen Mohammed Mahmud, Abdalrhman Falah Omar Ali
    European Journal of Medical and Health Research.2024; 2(4): 257.     CrossRef
  • Infección por Toxoplasma gondii como factor de riesgo para desarrollar esquizofrenia: revisión de la literatura
    Felipe Botero Rodríguez, Ana María Zárate, Damaris Cote Martínez, Carlos Gómez Restrepo, Andrés Duarte Osorio
    Universitas Médica.2023;[Epub]     CrossRef
  • Metabolomic Profiling Reveals Common Metabolic Alterations in Plasma of Patients with Toxoplasma Infection and Schizophrenia
    Emelia Osman, Anis Safirah Mohammad Zahariluddin, Shalisah Sharip, Zulkarnain Md Idris, Jen Kit Tan
    Genes.2022; 13(8): 1482.     CrossRef
  • Association between exposure to toxoplasmosis and major psychiatric disorders: a systematic review
    Santiago M. Fernandes, Alan R. Dias, Ângela Miranda-Scippa
    Brazilian Journal of Psychiatry.2021; 43(4): 438.     CrossRef
  • Seroprevalence of ToRCH Pathogens in Southeast Asia
    Franziska E. Fuchs, Maude Pauly, Antony P. Black, Judith M. Hübschen
    Microorganisms.2021; 9(3): 574.     CrossRef
  • A Review on the Prevalence of Toxoplasma gondii in Humans and Animals Reported in Malaysia from 2008–2018
    Mohammed Nasiru Wana, Mohamad Aris Mohd Moklas, Malaika Watanabe, Norshariza Nordin, Ngah Zasmy Unyah, Sharif Alhassan Abdullahi, Ashraf Ahmad Issa Alapid, Tijjani Mustapha, Rusliza Basir, Roslaini Abd. Majid
    International Journal of Environmental Research and Public Health.2020; 17(13): 4809.     CrossRef
  • Molecular Detection and Genetic Diversity of Toxoplasma gondii Oocysts in Cat Faeces from Klang Valley, Malaysia, Using B1 and REP Genes in 2018
    Mohammed Nasiru Wana, Mohamad Aris Mohd Moklas, Malaika Watanabe, Ngah Zasmy Unyah, Sharif Alhassan Abdullahi, Ashraf Ahmad Issa Alapid, Norshariza Nordin, Rusliza Basir, Roslaini Abd Majid
    Pathogens.2020; 9(7): 576.     CrossRef
  • Depression and Toxoplasma gondii infection: assess the possible relationship through a seromolecular case–control study
    Saber Nasirpour, Farnaz Kheirandish, Shirzad Fallahi
    Archives of Microbiology.2020; 202(10): 2689.     CrossRef
  • Toxoplasmosis in a Cohort of Italian Patients With Bipolar and Psychotic Disorders
    Claudia Del Grande, Elisa Schiavi, Isabella Masci, Margherita Barbuti, Giuseppe Maccariello, Gabriele Massimetti, Fabrizio Bruschi, Liliana Dell'Osso
    Journal of Nervous & Mental Disease.2020; 208(2): 118.     CrossRef
  • Cerebral Malaria and Toxoplasmosis: Could their Concomitant Presentation Worsen Psychotic Condition?
    Mohammed A. Ibrahim, Olayinka Atilola, Aminu Mohammed, Emmanuel J. Awosanya, Ismail A. Odetokun, Aliyu Muhammad, Chinwe U. Chukwudi, Kingsley N. Ukwaja, Oyetunde T. Oyeyemi
    Annals of Science and Technology.2020; 5(2): 24.     CrossRef
  • Serological and molecular detection of Toxoplasma gondii in terrestrial and marine wildlife harvested for food in Nunavik, Canada
    Nicholas Bachand, André Ravel, Patrick Leighton, Craig Stephen, Momar Ndao, Ellen Avard, Emily Jenkins
    Parasites & Vectors.2019;[Epub]     CrossRef
  • Lack of circulating toxoplasma gondii DNA in seropositive patients with bipolar or schizophrenia spectrum disorders
    L. Galli, C. Del Grande, L. Rindi, C. Mangia, V. Mangano, E. Schiavi, I. Masci, B. Pinto, L. Kramer, L. Dell'Osso, F. Bruschi
    Psychiatry Research.2019; 273: 706.     CrossRef
  • A Case–Control Seroprevalence Study on the Association Between Toxoplasma gondii Infection and Bipolar Disorder
    Cosme Alvarado-Esquivel, Sergio Estrada-Martínez, Alma Rosa Pérez-Alamos
    Frontiers in Psychiatry.2019;[Epub]     CrossRef
  • Associations of mental disorders and neurotropic parasitic diseases: a meta-analysis in developing and emerging countries
    Labanté Outcha Daré, Pierre-Emile Bruand, Daniel Gérard, Benoît Marin, Valerie Lameyre, Farid Boumédiène, Pierre-Marie Preux
    BMC Public Health.2019;[Epub]     CrossRef
  • Foxes (Vulpes vulpes) as sentinels for parasitic zoonoses, Toxoplasma gondii and Trichinella nativa, in the northeastern Canadian Arctic
    Bachand Nicholas, A. Ravel, P. Leighton, C. Stephen, A. Iqbal, M. Ndao, K. Konecsni, C. Fernando, E. Jenkins
    International Journal for Parasitology: Parasites and Wildlife.2018; 7(3): 391.     CrossRef
  • Possible Link Between Toxoplasma gondii Infection and Mood Disorders in Lorestan Province, Western Iran
    Farnaz Kheirandish, Hedayat Nazari, Hossein Mahmoudvand, Yaser Yaseri, Mohammad Javad Tarahi, Shirzad Fallahi, Behrouz Ezatpour
    Archives of Clinical Infectious Diseases.2016;[Epub]     CrossRef
  • Mental Health Disorders Associated with Foodborne Pathogens
    Declan J. Bolton, Lucy J. Robertson
    Journal of Food Protection.2016; 79(11): 2005.     CrossRef
  • 13,360 View
  • 112 Download
  • 24 Web of Science
  • Crossref

Brief Communication

Prevalence and Genetic Characterization of Toxoplasma gondii in Pet Dogs in Central China
Wei-Feng Qian, Wen-Chao Yan, Tian-Qi Wang, Kai Zhai, Li-Fang Han, Chao-Chao Lv
Korean J Parasitol 2015;53(1):125-128.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.125

The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3’+5’) SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.

Citations

Citations to this article as recorded by  Crossref logo
  • Global geographical distribution of Toxoplasma gondii genotypes from dogs: A systematic review
    Maryam Hataminejad, Mahbobeh Montazeri, Asal Tanzifi, Tahereh Mikaeili Galeh
    Parasite Epidemiology and Control.2025; 30: e00433.     CrossRef
  • Can cloning and sequencing help to genotype positive Toxoplasma gondii clinical samples? Results and validation using SAG3 as a model
    Claudia Patricia Rico-Torres, Luis Fernando Valenzuela-Moreno, Sara Teresa Méndez-Cruz, Carlos Cedillo-Peláez, Heriberto Caballero-Ortega
    Infection, Genetics and Evolution.2022; 101: 105283.     CrossRef
  • Low Prevalence of Toxoplasma gondii in Dogs From Central China
    Niuping Zhu, Liulu Yang, Shilin Xin, Wei Huang, Yibao Jiang, Yurong Yang
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • Molecular Detection and Genotyping ofToxoplasma gondiiandNeospora caninumin Slaughtered Goats in Central China
    Weifeng Qian, Wenchao Yan, Chaochao Lv, Rongzhen Bai, Tianqi Wang, Zhiguo Wei, Min Zhang
    Foodborne Pathogens and Disease.2020; 17(5): 348.     CrossRef
  • A Review on the Prevalence of Toxoplasma gondii in Humans and Animals Reported in Malaysia from 2008–2018
    Mohammed Nasiru Wana, Mohamad Aris Mohd Moklas, Malaika Watanabe, Norshariza Nordin, Ngah Zasmy Unyah, Sharif Alhassan Abdullahi, Ashraf Ahmad Issa Alapid, Tijjani Mustapha, Rusliza Basir, Roslaini Abd. Majid
    International Journal of Environmental Research and Public Health.2020; 17(13): 4809.     CrossRef
  • Toxoplasma gondii infections in dogs: 2009-2020
    Jitender P. Dubey, Fernando H.A. Murata, Camila K. Cerqueira-Cézar, Oliver C.H. Kwok, Yurong Yang, Chunlei Su
    Veterinary Parasitology.2020; 287: 109223.     CrossRef
  • Molecular Detection and Genetic Characterization of Toxoplasma gondii from Horses in Three Provinces of China
    Wei-Xin Ren, Xiao-Xuan Zhang, Cui-Yu Long, Quan Zhao, Tian Cheng, Jian-Gang Ma, Peng Xu, Guangyu Hou, Hong-Bo Ni
    Vector-Borne and Zoonotic Diseases.2019; 19(9): 703.     CrossRef
  • Occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in slaughtered domestic rabbits in central China
    Weifeng Qian, Wenchao Yan, Chaochao Lv, Rongzhen Bai, Tianqi Wang
    Parasite.2019; 26: 36.     CrossRef
  • Prevalence, risk factors and genetic characterization of Toxoplasma gondii in sick pigs and stray cats in Jiangsu Province, eastern China
    Zhao-feng Hou, Shi-jie Su, Dan-dan Liu, Le-le Wang, Chuan-li Jia, Zhen-xing Zhao, Yi-fei Ma, Qiao-qiao Li, Jin-jun Xu, Jian-ping Tao
    Infection, Genetics and Evolution.2018; 60: 17.     CrossRef
  • Geographical distribution of Toxoplasma gondii genotypes in Asia: A link with neighboring continents
    P. Chaichan, A. Mercier, L. Galal, A. Mahittikorn, F. Ariey, S. Morand, F. Boumédiène, R. Udonsom, A. Hamidovic, J.B. Murat, Y. Sukthana, M.L. Dardé
    Infection, Genetics and Evolution.2017; 53: 227.     CrossRef
  • Prevalence and genetic characterization of Toxoplasma gondii in donkeys in northeastern China
    Xiao-Xuan Zhang, Wei Shi, Nian-Zhang Zhang, Kun Shi, Jian-Ming Li, Peng Xu, Quan Zhao, Rui Du
    Infection, Genetics and Evolution.2017; 54: 455.     CrossRef
  • Seroprevalence and genotypes of Toxoplasma gondii isolated from pigs intended for human consumption in Liaoning province, northeastern China
    Dawei Wang, Yan Liu, Tiantian Jiang, Guoxin Zhang, Gaoming Yuan, Jianbin He, Chunlei Su, Na Yang
    Parasites & Vectors.2016;[Epub]     CrossRef
  • Seroprevalence ofToxoplasma gondiiin goats in Hunan province, China
    Fen Li, Shi-Ping Wang, Chang-Jian Wang, Shi-Cheng He, Xiang Wu, Guo-Hua Liu
    Parasite.2016; 23: 44.     CrossRef
  • Prevalence of <i>Toxoplasma gondii</i> in Dogs in Zhanjiang, Southern China
    Hai-Hai Jiang, Ming-Wei Li, Min-Jun Xu, Wei Cong, Xing-Quan Zhu
    The Korean Journal of Parasitology.2015; 53(4): 493.     CrossRef
  • Seroprevalence and risk factors of Toxoplasma gondii infection in stray dogs in northern China
    Xiao-Xuan Zhang, Ya-Nan Cai, Chun-Feng Wang, Jing Jiang, Ying-Tian Xu, Gui-Lian Yang, Quan Zhao
    Parasitology Research.2015; 114(12): 4725.     CrossRef
  • 12,550 View
  • 103 Download
  • 16 Web of Science
  • Crossref

Original Article

Prominent IL-12 Production and Tumor Reduction in Athymic Nude Mice after Toxoplasma gondii Lysate Antigen Treatment
Kyoung-Ho Pyo, Bong-Kwang Jung, Chun-Feng Xin, You-Won Lee, Jong-Yil Chai, Eun-Hee Shin
Korean J Parasitol 2014;52(6):605-612.
Published online December 23, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.6.605

Toxoplasma gondii is an intracellular protozoan parasite that causes a Th1 cellular immunity. Our previous study showed that T. gondii lysate antigen (TLA) treatment in S180 tumor-bearing mice resulted in tumor reduction by suppressing CD31 expression, a marker of angiogenesis. In the present study, to investigate tumor suppressive effect of TLA under the absence of T lymphocytes, athymic nude mice were compared with euthymic mice in the anti-tumorigenic effect triggered by TLA in CT26 tumors. According to the results, intratumorally injected TLA reduced tumor growth and TIMP-1 level, a metastatic marker, in both euthymic and athymic mice. TLA treatment led to a sharp increase in IL-12 expression in serum cytokine profiling of athymic mice, and increased MyD88 signals in macrophages derived from the bone marrow, implying the activation of innate immunity. The selective induction of IL-12 by TLA treatment had an anti-tumorigenic effect.

Citations

Citations to this article as recorded by  Crossref logo
  • An Overview of the Dichotomous Role of Microbiota in Cancer Progression and Management
    Pooja Jain, Sradhanjali Mohapatra, Uzma Farooq, Nazia Hassan, Mohd. Aamir Mirza, Zeenat Iqbal
    Current Cancer Drug Targets.2025; 25(1): 38.     CrossRef
  • Bug as a drug: Unveiling anti-cancer properties of Toxoplasma gondii and its therapeutic prospects in cancer immunotherapy
    Yie Wei Chua, Sek Chuen Chow
    Acta Tropica.2025; 267: 107684.     CrossRef
  • From pathogen to cure: exploring the antitumor potential of Toxoplasma gondii
    Parisa Alipanahi, Arezou Khosrojerdi, Abdol Satar Pagheh, Kareem Hatam-Nahavandi, Ehsan Ahmadpour
    Infectious Agents and Cancer.2025;[Epub]     CrossRef
  • Bridging the gap for diverse applications of parasites as advanced cancer therapeutics: current progress and future directions
    Maha M. Eissa, Marwa H. El-Faham, Nahla El Skhawy
    Infectious Agents and Cancer.2025;[Epub]     CrossRef
  • Evaluation of Antitumor Effects of Toxoplasma gondii Different Antigens on Ehrlich Solid Carcinoma in Mice
    Salwa S. Younis, Basma M. Elmansory, Hend A. Elrefaey, Nahla A. Nasef, Sara H. Elakshar, Radwa A. Awad, Ghada A. Gamea
    Parasite Immunology.2025;[Epub]     CrossRef
  • A novel enemy of cancer: recent investigations into protozoan anti-tumor properties
    Zhi Zheng, Xiao Lu, Dong Zhou, Xu-feng Deng, Quan-xing Liu, Xiao-bing Liu, Jiao Zhang, Yan-qi Li, Hong Zheng, Ji-gang Dai
    Frontiers in Cellular and Infection Microbiology.2024;[Epub]     CrossRef
  • Parasite-enhanced immunotherapy: transforming the “cold” tumors to “hot” battlefields
    Yujun Xie, Jinyan Wang, Yafei Wang, Yalin Wen, Yanping Pu, Benfan Wang
    Cell Communication and Signaling.2024;[Epub]     CrossRef
  • Parasites revive hope for cancer therapy
    Maha M. Eissa, Ahmed Ebada Salem, Nahla El Skhawy
    European Journal of Medical Research.2024;[Epub]     CrossRef
  • Toxoplasma gondii-Derived Exosomes: A Potential Immunostimulant and Delivery System for Tumor Immunotherapy Superior to Toxoplasma gondii
    Lai-Xi Zhao, Qiong Sun, Chong Wang, Jia-Jia Liu, Xiao-Rong Yan, Meng-Ci Shao, Li Yu, Wen-Hua Xu, Rui Xu
    International Journal of Nanomedicine.2024; Volume 19: 12421.     CrossRef
  • Association of Neuroblastoma (NB) SH-SY5Y Cells with Antibodies of Parasitic Origin (Anti-Acanthamoeba and Anti-Toxocara canis)
    Víctor Alberto Maravelez Acosta, Maria de Lourdes Caballero Garcia, Genaro Patiño López, María del Pilar Crisóstomo Vázquez, Luz Ofelia Franco Sandoval, Leticia Eligio García
    International Journal of Molecular Sciences.2024; 25(24): 13577.     CrossRef
  • Promising effects of parasite-derived compounds on tumor regression: a systematic review of in vitro and in vivo studies
    Ali Asghari, Hassan Nourmohammadi, Hamidreza Majidiani, Seyyed Ali Shariatzadeh, Davood Anvari, Sadegh Shamsinia, Ezatollah Ghasemi, Morteza Shams, Gholam Basati
    Environmental Science and Pollution Research.2022; 29(22): 32383.     CrossRef
  • Toxoplasma gondii infection possibly reverses host immunosuppression to restrain tumor growth
    Jiating Chen, Wenzhong Liao, HongJuan Peng
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • Neospora caninum inhibits tumor development by activating the immune response and destroying tumor cells in a B16F10 melanoma model
    Xiaojin Li, Meng Qi, Kai He, Haiyan Liu, Wenlan Yan, Lizhuo Zhao, Yanyan Jia, Lei He, Chaochao Lv, Min Zhang, Zhiguo Wei, Wenchao Yan, Tianqi Wang, Fuchang Yu, Weifeng Qian
    Parasites & Vectors.2022;[Epub]     CrossRef
  • Exploiting the Macrophage Production of IL-12 in Improvement of Vaccine Development against Toxoplasma gondii and Neospora caninum Infections
    Ragab M. Fereig, Mosaab A. Omar, Abdullah F. Alsayeqh
    Vaccines.2022; 10(12): 2082.     CrossRef
  • Toxoplasma GRA16 Inhibits NF-κB Activation through PP2A-B55 Upregulation in Non-Small-Cell Lung Carcinoma Cells
    Seung-Hwan Seo, Sang-Gyun Kim, Ji-Hun Shin, Do-Won Ham, Eun-Hee Shin
    International Journal of Molecular Sciences.2020; 21(18): 6642.     CrossRef
  • Increase in the nuclear localization of PTEN by the Toxoplasma GRA16 protein and subsequent induction of p53‐dependent apoptosis and anticancer effect
    Sang‐Gyun Kim, Seung‐Hwan Seo, Ji‐Hun Shin, Jung‐Pyo Yang, Sang Hyung Lee, Eun‐Hee Shin
    Journal of Cellular and Molecular Medicine.2019; 23(5): 3234.     CrossRef
  • Characteristics of Infection Immunity Regulated by Toxoplasma gondii to Maintain Chronic Infection in the Brain
    Young Sang Hwang, Ji-Hun Shin, Jung-Pyo Yang, Bong-Kwang Jung, Sang Hyung Lee, Eun-Hee Shin
    Frontiers in Immunology.2018;[Epub]     CrossRef
  • Microorganisms in the Treatment of Cancer: Advantages and Limitations
    Klaudia Łukasiewicz, Marek Fol
    Journal of Immunology Research.2018; 2018: 1.     CrossRef
  • The Neurotropic Parasite Toxoplasma gondii Induces Sustained Neuroinflammation with Microvascular Dysfunction in Infected Mice
    Vanessa Estato, Joice Stipursky, Fabiana Gomes, Tally C. Mergener, Edwards Frazão-Teixeira, Silvana Allodi, Eduardo Tibiriçá, Helene S. Barbosa, Daniel Adesse
    The American Journal of Pathology.2018; 188(11): 2674.     CrossRef
  • Puzzling and ambivalent roles of malarial infections in cancer development and progression
    ERIC FAURE
    Parasitology.2016; 143(14): 1811.     CrossRef
  • NaHCO3 enhances the antitumor activities of cytokine-induced killer cells against hepatocellular carcinoma HepG2 cells
    Ya Hong Yuan, Chun Fang Zhou, Jiang Yuan, Li Liu, Xing Rong Guo, Xiao Li Wang, Yan Ding, Xiao Nan Wang, Dong Sheng Li, Han Jun Tu
    Oncology Letters.2016; 12(5): 3167.     CrossRef
  • Immune adjuvant effect of aToxoplasma gondiiprofilin-like protein in autologous whole-tumor-cell vaccination in mice
    Kyoung-Ho Pyo, You-Won Lee, Sun Min Lim, Eun-Hee Shin
    Oncotarget.2016; 7(45): 74107.     CrossRef
  • 14,290 View
  • 87 Download
  • 23 Web of Science
  • Crossref

Mini Review

Recent Advances in Toxoplasma gondii Immunotherapeutics
Sherene Swee-Yin Lim, Rofina Yasmin Othman
Korean J Parasitol 2014;52(6):581-593.
Published online December 23, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.6.581

Toxoplasmosis is an opportunistic infection caused by the protozoan parasite Toxoplasma gondii. T. gondii is widespread globally and causes severe diseases in individuals with impaired immune defences as well as congenitally infected infants. The high prevalence rate in some parts of the world such as South America and Africa, coupled with the current drug treatments that trigger hypersensitivity reactions, makes the development of immunotherapeutics intervention a highly important research priority. Immunotherapeutics strategies could either be a vaccine which would confer a pre-emptive immunity to infection, or passive immunization in cases of disease recrudescence or recurrent clinical diseases. As the severity of clinical manifestations is often greater in developing nations, the development of well-tolerated and safe immunotherapeutics becomes not only a scientific pursuit, but a humanitarian enterprise. In the last few years, much progress has been made in vaccine research with new antigens, novel adjuvants, and innovative vaccine delivery such as nanoparticles and antigen encapsulations. A literature search over the past 5 years showed that most experimental studies were focused on DNA vaccination at 52%, followed by protein vaccination which formed 36% of the studies, live attenuated vaccinations at 9%, and heterologous vaccination at 3%; while there were few on passive immunization. Recent progress in studies on vaccination, passive immunization, as well as insights gained from these immunotherapeutics is highlighted in this review.

Citations

Citations to this article as recorded by  Crossref logo
  • In silico analysis and structural vaccinology prediction of Toxoplasma gondii ROP41 gene via immunoinformatics methods as a vaccine candidate
    Masoumeh Asadi, Ali Dalir Ghaffari, Fatemeh Mohammadhasani
    Current Research in Translational Medicine.2025; 73(1): 103475.     CrossRef
  • Toxoplasma gondii (GRA10): A Closer Glance Into Immunogenic and Biochemical Characteristics Using In Silico Approach
    Mahdi Khadem Mohammadi, Ali Dalir Ghaffari
    Parasite Immunology.2025;[Epub]     CrossRef
  • Design and immunological evaluation of a multi-epitope vaccine candidate against Toxoplasma gondii incorporating MIC13, GRA1, and SAG1 antigens in BALB/c mice
    Zahra Hosseininejad, Ahmad Daryani, Mahdi Fasihi-Ramandi, Hossein Asgarian-Omran, Tooran Nayeri, Samira Dodangeh, Afsaneh Amouei, Javad Javidnia, Sabah Mayahi, Shahabeddin Sarvi, Sargis A. Aghayan
    Food and Waterborne Parasitology.2025; 40: e00269.     CrossRef
  • Effective factors in the pathogenesis of Toxoplasma gondii
    Tooran Nayeri, Shahabeddin Sarvi, Ahmad Daryani
    Heliyon.2024; 10(10): e31558.     CrossRef
  • Nanoparticles as a Delivery System of Antigens for the Development of an Effective Vaccine against Toxoplasma gondii
    Carina Brito, Camila Lourenço, Joana Magalhães, Salette Reis, Margarida Borges
    Vaccines.2023; 11(4): 733.     CrossRef
  • Metallic Nanoparticles and Core-Shell Nanosystems in the Treatment, Diagnosis, and Prevention of Parasitic Diseases
    Grzegorz Król, Kamila Fortunka, Michał Majchrzak, Ewelina Piktel, Paulina Paprocka, Angelika Mańkowska, Agata Lesiak, Maciej Karasiński, Agnieszka Strzelecka, Bonita Durnaś, Robert Bucki
    Pathogens.2023; 12(6): 838.     CrossRef
  • Toxoplasmosis: stages of the protozoan life cycle and risk assessment in humans and animals for an enhanced awareness and an improved socio-economic status
    Esam S. Al-Malki
    Saudi Journal of Biological Sciences.2021; 28(1): 962.     CrossRef
  • Synergistic effect of GRA7 and profilin proteins in vaccination against chronic Toxoplasma gondii infection
    Nadia Arcon, Mariano S. Picchio, Ignacio M. Fenoy, Rosalía E. Moretta, Ariadna S. Soto, Matías D. Perrone Sibilia, Vanesa R. Sánchez, Cecilia A. Prato, María Virginia Tribulatti, Alejandra Goldman, Valentina Martin
    Vaccine.2021; 39(6): 933.     CrossRef
  • Evaluation of immunogenicity and protection of the Mic1-3 knockout Toxoplasma gondii live attenuated strain in the feline host
    Delphine Le Roux, Vitomir Djokic, Solen Morisse, Clément Chauvin, Vanessa Doré, Anne-Claire Lagrée, Déborah Voisin, Yohan Villain, Aurélie Grasset-Chevillot, Fanny Boursin, Chunlei Su, Sébastien Perrot, Isabelle Vallée, Edouard Seche, Radu Blaga
    Vaccine.2020; 38(6): 1457.     CrossRef
  • A Review on the Prevalence of Toxoplasma gondii in Humans and Animals Reported in Malaysia from 2008–2018
    Mohammed Nasiru Wana, Mohamad Aris Mohd Moklas, Malaika Watanabe, Norshariza Nordin, Ngah Zasmy Unyah, Sharif Alhassan Abdullahi, Ashraf Ahmad Issa Alapid, Tijjani Mustapha, Rusliza Basir, Roslaini Abd. Majid
    International Journal of Environmental Research and Public Health.2020; 17(13): 4809.     CrossRef
  • In Silico Prediction of T and B Cell Epitopes of SAG1-Related Sequence 3 (SRS3) Gene for Developing Toxoplasma gondii Vaccine
    Abolfazl Mirzadeh, Geita Saadatnia, Majid Golkar, Jalal Babaie, Samira Amiri, Asiyeh Yoosefy
    Archives of Clinical Infectious Diseases.2020;[Epub]     CrossRef
  • Rhoptry antigens asToxoplasma gondiivaccine target
    Masoud Foroutan, Fatemeh Ghaffarifar, Zohreh Sharifi, Abdolhosein Dalimi, Ogholniaz Jorjani
    Clinical and Experimental Vaccine Research.2019; 8(1): 4.     CrossRef
  • Treatment of toxoplasmosis: Current options and future perspectives
    Neda Konstantinovic, Hélène Guegan, Tijana Stäjner, Sorya Belaz, Florence Robert-Gangneux
    Food and Waterborne Parasitology.2019; 15: e00036.     CrossRef
  • In silico analysis and expression of a novel chimeric antigen as a vaccine candidate against Toxoplasma gondii
    Samira Dodangeh, Mahdi Fasihi-Ramandi, Ahmad Daryani, Reza Valadan, Shahabeddin Sarvi
    Microbial Pathogenesis.2019; 132: 275.     CrossRef
  • Evaluation of Anti-Toxoplasma gondii Immune Responses in BALB/c Mice Induced by DNA Vaccines Encoding Surface Antigen 1 (SAG1) and 3 (SAG3)
    H. Sobati, A. Dalimi, B. Kazemi, F. Ghaffarifar
    Molecular Genetics, Microbiology and Virology.2019; 34(1): 59.     CrossRef
  • The course of infection with Toxoplasma gondii RH strain in mice pre-vaccinated with gamma irradiated tachyzoites
    Ahmed M. Gomaa, Nora L. El-Tantawy, Aliaa M. Elsawey, Ramy A. Abdelsalam, Manar S. Azab
    Experimental Parasitology.2019; 205: 107733.     CrossRef
  • Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts byToxoplasma gondii
    Sherene Swee Yin Lim, Kek Heng Chua, Greta Nölke, Holger Spiegel, Wai Leong Goh, Sek Chuen Chow, Boon Pin Kee, Rainer Fischer, Stefan Schillberg, Rofina Yasmin Othman
    PeerJ.2018; 6: e5780.     CrossRef
  • Characterization of a multi-epitope peptide with selective MHC-binding capabilities encapsulated in PLGA nanoparticles as a novel vaccine candidate against Toxoplasma gondii infection
    Mona Roozbehani, Reza Falak, Mohsen Mohammadi, Andrew Hemphill, Elham Razmjou, Ahmad reza Meamar, Leila Masoori, Majid Khoshmirsafa, Maryam Moradi, Mohammad Javad Gharavi
    Vaccine.2018; 36(41): 6124.     CrossRef
  • Synergic in vitro combinations of artemisinin, pyrimethamine and methylene blue against Neospora caninum
    Luiz Miguel Pereira, Gabriela de Luca, Nathália de Lima Martins Abichabki, Cássia Mariana Bronzon da Costa, Ana Patrícia Yatsuda
    Veterinary Parasitology.2018; 249: 92.     CrossRef
  • Vaccine potential of antigen cocktails composed of recombinant Toxoplasma gondii TgPI-1, ROP2 and GRA4 proteins against chronic toxoplasmosis in C3H mice
    Mariano S. Picchio, Vanesa R. Sánchez, Nadia Arcon, Ariadna S. Soto, Matías Perrone Sibilia, María de los Angeles Aldirico, Mariela Urrutia, Rosalía Moretta, Ignacio M. Fenoy, Alejandra Goldman, Valentina Martin
    Experimental Parasitology.2018; 185: 62.     CrossRef
  • Calcium-dependent protein kinases are potential targets forToxoplasma gondiivaccine
    Masoud Foroutan, Fatemeh Ghaffarifar
    Clinical and Experimental Vaccine Research.2018; 7(1): 24.     CrossRef
  • Recent progress in microneme-based vaccines development againstToxoplasma gondii
    Masoud Foroutan, Leila Zaki, Fatemeh Ghaffarifar
    Clinical and Experimental Vaccine Research.2018; 7(2): 93.     CrossRef
  • A Systematic Review of In vitro and In vivo Activities of Anti-Toxoplasma Drugs and Compounds (2006–2016)
    Mahbobeh Montazeri, Mehdi Sharif, Shahabeddin Sarvi, Saeed Mehrzadi, Ehsan Ahmadpour, Ahmad Daryani
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • The genome of the protozoan parasite Cystoisospora suis and a reverse vaccinology approach to identify vaccine candidates
    Nicola Palmieri, Aruna Shrestha, Bärbel Ruttkowski, Tomas Beck, Claus Vogl, Fiona Tomley, Damer P. Blake, Anja Joachim
    International Journal for Parasitology.2017; 47(4): 189.     CrossRef
  • Immunization with Toxoplasma gondii aspartic protease 3 increases survival time of infected mice
    Guanghui Zhao, Xiaojie Song, Xiangnan Kong, Ning Zhang, Shaoling Qu, Wei Zhu, Yanyan Yang, Qian Wang
    Acta Tropica.2017; 171: 17.     CrossRef
  • Vaccination with recombinant adenovirus expressing multi-stage antigens ofToxoplasma gondiiby the mucosal route induces higher systemic cellular and local mucosal immune responses than with other vaccination routes
    Ting Wang, Huiquan Yin, Yan Li, Lingxiao Zhao, Xiahui Sun, Hua Cong
    Parasite.2017; 24: 12.     CrossRef
  • An evaluation of a recombinant multiepitope based antigen for detection of Toxoplasma gondii specific antibodies
    Khalid Hajissa, Robaiza Zakaria, Rapeah Suppian, Zeehaida Mohamed
    BMC Infectious Diseases.2017;[Epub]     CrossRef
  • Vaccination against parasites – status quo and the way forward
    Anja Joachim
    Porcine Health Management.2016;[Epub]     CrossRef
  • Immunization of Wistar female rats with 255-Gy-irradiated Toxoplasma gondii: Tissue parasitic load and lactogenic quantification
    Lucilene Granuzzio Camossi, Felipe Fornazari, Virgínia Bodelão Richini-Pereira, Rodrigo Costa da Silva, Daniel Fontana Ferreira Cardia, Helio Langoni
    Experimental Parasitology.2015; 154: 163.     CrossRef
  • Homologous prime-boost strategy with TgPI-1 improves the immune response and protects highly susceptible mice against chronic Toxoplasma gondii infection
    Vanesa R. Sánchez, Ignacio M. Fenoy, Mariano S. Picchio, Ariadna S. Soto, Nadia Arcon, Alejandra Goldman, Valentina Martin
    Acta Tropica.2015; 150: 159.     CrossRef
  • Seroprevalence of <i>Toxoplasma gondii</i> Among Primary School Children in Shandong Province, China
    Ke-Sheng Xin, Hui Liu, Hong-Bing Wang, Zong-Liang Yao
    The Korean Journal of Parasitology.2015; 53(4): 489.     CrossRef
  • In Vitro and In Vivo Effects of the Bumped Kinase Inhibitor 1294 in the Related Cyst-Forming Apicomplexans Toxoplasma gondii and Neospora caninum
    Pablo Winzer, Joachim Müller, Adriana Aguado-Martínez, Mahbubur Rahman, Vreni Balmer, Vera Manser, Luis Miguel Ortega-Mora, Kayode K. Ojo, Erkang Fan, Dustin J. Maly, Wesley C. Van Voorhis, Andrew Hemphill
    Antimicrobial Agents and Chemotherapy.2015; 59(10): 6361.     CrossRef
  • Clinically Available Medicines Demonstrating Anti-Toxoplasma Activity
    Andrew J. Neville, Sydney J. Zach, Xiaofang Wang, Joshua J. Larson, Abigail K. Judge, Lisa A. Davis, Jonathan L. Vennerstrom, Paul H. Davis
    Antimicrobial Agents and Chemotherapy.2015; 59(12): 7161.     CrossRef
  • 14,430 View
  • 150 Download
  • 37 Web of Science
  • Crossref

Original Articles

A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women
Imen Khammari, Fatma Saghrouni, Sami Lakhal, Aida Bouratbine, Moncef Ben Said, Jalel Boukadida
Korean J Parasitol 2014;52(5):493-499.
Published online October 22, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.5.493

The determination of the accurate immune status of pregnant women is crucial in order to prevent congenital toxoplasmosis. Equivocal results with conventional serological techniques are not uncommon when IgG titers are close to the cut-off value of the test, so that a confirmatory technique is needed. For this purpose, we developed a homemade immunoblot (IB) using soluble extract of Toxoplasma gondii tachyzoites and assessed it by testing 154 positive, 100 negative, and 123 equivocal sera obtained from pregnant women. In order to select the more valuable bands in terms of sensitivity and specificity, we used the Youden Index (YI). The highest YIs were those given by the 32, 36, 98, 21, and 33 bands. The simultaneous presence on the same blot of at least 3 bands showed a much higher YI (0.964) and was adapted as the positivity criterion. The analysis of results showed that our homemade IB correlated well with the commercial LDBIO Toxo II IgG® kit recently recommended as a confirmatory test (96.7% of concordance).

Citations

Citations to this article as recorded by  Crossref logo
  • Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges
    Priscila Silva Franco, Ana Carolina Morais Oliveira Scussel, Rafaela José Silva, Thadia Evelyn Araújo, Henrique Tomaz Gonzaga, Camila Ferreira Marcon, Joaquim Pedro Brito-de-Sousa, Angélica Lemos Debs Diniz, Marina Carvalho Paschoini, Bellisa Freitas Barb
    Journal of Tropical Medicine.2024; 2024: 1.     CrossRef
  • Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection
    Min-ju Kim, Soeun J. Park, Hyunwoo Park
    European Journal of Medical Research.2024;[Epub]     CrossRef
  • Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
    Valeria Meroni, Francesca Genco, Luigia Scudeller, Marie-Pierre Brenier-Pinchart, Hélène Fricker-Hidalgo, Coralie L’Ollivier, Luc Paris, Hervé Pelloux
    Pathogens.2022; 11(6): 665.     CrossRef
  • Serological diagnosis of toxoplasmosis: evaluation of the commercial test recomLine Toxoplasma IgG immunoblot (Mikrogen) based on recombinant antigens
    Vincent Jean-Pierre, Julien Miozzo, Hélène Fricker-Hidalgo, Cécile Garnaud, Marie Gladys Robert, Hervé Pelloux, Marie-Pierre Brenier-Pinchart
    Parasite.2022; 29: 52.     CrossRef
  • A longitudinal study of Toxoplasma gondii seroconversion on four large Danish sow farms
    Abbey Olsen, Lis Alban, Matthew Denwood, Hans Houe, Tina Birk Jensen, Henrik Vedel Nielsen
    Veterinary Parasitology.2021; 295: 109460.     CrossRef
  • Performance of seven commercial automated assays for the detection of low levels of anti-Toxoplasma IgG in French immunocompromised patients
    Tiphaine Douet, Catherine Armengol, Elena Charpentier, Pamela Chauvin, Sophie Cassaing, Xavier Iriart, Antoine Berry, Judith Fillaux
    Parasite.2019; 26: 51.     CrossRef
  • Performance of Zika Assays in the Context of Toxoplasma gondii, Parvovirus B19, Rubella Virus, and Cytomegalovirus (TORCH) Diagnostic Assays
    Bettie Voordouw, Barry Rockx, Thomas Jaenisch, Pieter Fraaij, Philippe Mayaud, Ann Vossen, Marion Koopmans
    Clinical Microbiology Reviews.2019;[Epub]     CrossRef
  • Semiquantitative Dot Blot with the GRA8 antigen to differentiate the stages of toxoplasmosis infection
    Juan Gabriel Costa, María Julia Vilariño
    Journal of Microbiological Methods.2018; 149: 9.     CrossRef
  • Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico
    Cosme Alvarado-Esquivel, Luis Sánchez-Anguiano, Luis Berumen-Segovia, Jesús Hernández-Tinoco, Yazmin Rico-Almochantaf, Alfredo Cisneros-Camacho, Jorge Cisneros-Martínez
    International Journal of Environmental Research and Public Health.2017; 14(11): 1413.     CrossRef
  • Sensing parasites: Proteomic and advanced bio-detection alternatives
    Carlos Sánchez-Ovejero, Fernando Benito-Lopez, Paula Díez, Adriano Casulli, Mar Siles-Lucas, Manuel Fuentes, Raúl Manzano-Román
    Journal of Proteomics.2016; 136: 145.     CrossRef
  • The impact of lowering the cut-off value on the sensitivity of the Platelia Elisa IgG (Bio-Rad) test for toxoplasmosis diagnosis
    Oussama Mouri, Eric Kendjo, Feriel Touafek, Arnaud Fekkar, Ousmane Konte, Sebastien Imbert, Régis Courtin, Dominique Mazier, Luc Paris
    Parasite.2015; 22: 22.     CrossRef
  • 10,027 View
  • 117 Download
  • 12 Web of Science
  • Crossref
IgG Avidity Antibodies against Toxoplasma gondii in High Risk Females of Reproductive Age Group in India
Naushaba Siddiqui, Fatima Shujatullah, Haris M. Khan, Tamkin Rabbani, Parvez A. Khan
Korean J Parasitol 2014;52(5):487-491.
Published online October 22, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.5.487

Toxoplasma gondii is an obligate intracellular protozoan that is distributed worldwide. Recently, several tests for avidity of Toxoplasma IgG antibodies have been introduced to help discriminate between recently acquired and distant infections. The study was conducted in Jawaharlal Nehru Medical College and Hospital, India from February 2011 to September 2012. Serum specimens were subjected to Toxoplasma IgM ELISA and IgG avidity ELISA test. Out of 48 patients with abortions, 17 (35.4%) were positive for IgM ELISA, and 8 (16.6%) had low IgG avidity antibodies. Out of 48 patients with other obstetric problems, 23 (47.9%) were positive for IgM ELISA, and 17 (35.4%) had low IgG avidity antibodies. Combining both groups on avidity test, only 25 of 40 (62.5%) IgM-positive women had low-avidity IgG antibodies suggesting a recent T. gondii infection in these women. More importantly, 15 (37.5%) of the IgM-positive women had high-avidity antibodies suggesting that the infection was acquired before gestation The relation of IgM seropositivity with the following risk factors was not found to be statistically significant; contact with cats (0.13), non-vegetarian food habits (0.05), and low socio-economic status (0.49). While, for IgG avidity ELISA, only contact with cats (0.01) was significantly associated with seropositivity. All other risk factors have P-values of >0.05 (not significant). IgG avidity test when used in combination with IgM test was a valuable assay for diagnosis of ongoing or recently acquired T. gondii infection in India.

Citations

Citations to this article as recorded by  Crossref logo
  • A meta-analysis and survey on the prevalence of Toxoplasma gondii infection in cats (Felis catus)
    Mohd Ubaid, Umra Fatima Zuberi, Syed Muhammad Sarosh Ghalib, Faizeaab Hashmi, Sana Aqeel
    The Journal of Basic and Applied Zoology.2025;[Epub]     CrossRef
  • Seroprevalence and associated behavioral factors of Toxoplasma gondii infection among pregnant women in Pokhara Valley, Nepal
    Mamata Thapa, Tulsi Ram Gompo, Tatsuki Sugi, Masahito Asada, Hiroaki Arima, Kishor Pandey
    Tropical Medicine and Health.2025;[Epub]     CrossRef
  • Relationship between the serum level, polymorphism and gene expression of IL-33 in samples of recurrent miscarriage Iraqi women infected with toxoplasmosis
    Sabreen Hadi Fadhil, Entsar Jabbar Saheb
    Experimental Parasitology.2024; 263-264: 108799.     CrossRef
  • Detection of persistent low IgG avidity–an interpretative problem in the diagnosis of acute toxoplasmosis
    Petr Kodym, Zuzana Kurzová, Dagmar Berenová, Marek Malý, Gheyath K. Nasrallah
    PLOS ONE.2023; 18(4): e0284499.     CrossRef
  • Diagnosis of Acute Toxoplasmosis in the First Trimester of Pregnancy by IgG Avidity
    Afshin Azimi, Mahdi Parsaei, Eissa Soleymani, Elham Mehranzadeh, Amir Hossein Maghsood
    Avicenna Journal of Clinical Medicine.2022; 29(1): 50.     CrossRef
  • Toxoplasma gondii infection and spontaneous abortion: A systematic review and meta-analysis
    Narges Kalantari, Tahmineh Gorgani-Firouzjaee, Zahra Moulana, Mohammad Chehrazi, Salman Ghaffari
    Microbial Pathogenesis.2021; 158: 105070.     CrossRef
  • The global seroprevalence of anti-Toxoplasma gondii antibodies in women who had spontaneous abortion: A systematic review and meta-analysis
    Tooran Nayeri, Shahabeddin Sarvi, Mahmood Moosazadeh, Afsaneh Amouei, Zahra Hosseininejad, Ahmad Daryani, Maria Angeles Gómez-Morales
    PLOS Neglected Tropical Diseases.2020; 14(3): e0008103.     CrossRef
  • Analysis Concentration of <i>Toxoplasma gondii</i> on Anti-Toxoplasma IgG-IgM Antibody Levels, and the Outcomes of Pregnancy in Mice Balb/c
    Tigor Peniel Simanjuntak, Mochammad Hatta, Robert H. Sirait, Marni Br Karo, Lenny Irmawaty Sirait, Tetty Rina Aritonang, Syahrul Rauf, Ressy Dwiyanti
    Open Journal of Obstetrics and Gynecology.2017; 07(03): 281.     CrossRef
  • Seroprevalence of Toxoplasma gondii among pregnant women in Almadinah Almunawwarah KSA
    Naglaa F.A. Imam, Esra'a A.A. Azzam, Ahmed A. Attia
    Journal of Taibah University Medical Sciences.2016; 11(3): 255.     CrossRef
  • 9,249 View
  • 83 Download
  • 9 Web of Science
  • Crossref

Brief Communication

Gefitinib Inhibits the Growth of Toxoplasma gondii in HeLa Cells
Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
Korean J Parasitol 2014;52(4):439-441.
Published online August 29, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.4.439

Toxoplasma gondii is the causative agent of toxoplasmosis with symptoms of congenital neurological and ocular diseases and acquired lymphadenitis, retinochoroiditis, and meningoencephalitis. Small molecules which block the activity of protein kinases were tested in in vitro culture of T. gondii to find new therapeutic drugs of safer and more effective than the combined administration of pyrimethamine and sulfadoxine that sometimes provoke lethal Stevens-Johnson syndrome. Among them, Gefitinib and Crizotinib inhibited intracellular growth of T. gondii in HeLa cells by counting the number of T. gondii per parasitophorous vacuolar membrane whereas Sunitinib did not. Gefitinib inhibited the growth of T. gondii in a dose-dependent manner over 5 ?M up to the tolerable concentration of HeLa cells and halted the division of the parasite immediately from the time point of treatment. Gefitinib inhibition suggests that tyrosine kinases of EGFR family or other homologous kinases of the parasite itself may be the target to cause the block of T. gondii growth.

Citations

Citations to this article as recorded by  Crossref logo
  • Experimental and clinical tests of FDA-approved kinase inhibitors for the treatment of neurological disorders (update 2024)
    Hassan Aliashrafzadeh, Dewey Liu, Samantha De Alba, Imad Akbar, Austin Lui, Jordan Vanleuven, Ryan Martin, Zhang Wang, Da Zhi Liu
    Exploration of Drug Science.2025;[Epub]     CrossRef
  • The antimicrobial activity of innate host-directed therapies: A systematic review
    Tirosh Shapira, Matthew Christofferson, Yossef Av-Gay
    International Journal of Antimicrobial Agents.2024; 63(5): 107138.     CrossRef
  • Impact of Training Data, Ground Truth and Shape Variability in the Deep Learning-Based Semantic Segmentation of HeLa Cells Observed with Electron Microscopy
    Cefa Karabağ, Mauricio Alberto Ortega-Ruíz, Constantino Carlos Reyes-Aldasoro
    Journal of Imaging.2023; 9(3): 59.     CrossRef
  • Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathway
    Xue-song Zhang, Yong-juan Zhao, Yu Zhang, Tong Xu, Kai-ling Cui, Bu-ting Duan, Xiao-ling Lv, Li Zhang, Zhi-yong Xu, Rui Bai, Ming-xue Zheng
    Poultry Science.2022; 101(10): 102075.     CrossRef
  • FDA-Approved Kinase Inhibitors in Preclinical and Clinical Trials for Neurological Disorders
    Austin Lui, Jordan Vanleuven, David Perekopskiy, Dewey Liu, Desiree Xu, Omar Alzayat, Taiseer Elgokhy, Timothy Do, Meghan Gann, Ryan Martin, Da-Zhi Liu
    Pharmaceuticals.2022; 15(12): 1546.     CrossRef
  • Clearing or subverting the enemy: Role of autophagy in protozoan infections
    George Ghartey-Kwansah, Benjamin Aboagye, Frank Adu-Nti, Yeboah Kwaku Opoku, Emmanuel Kwasi Abu
    Life Sciences.2020; 247: 117453.     CrossRef
  • Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors
    Hye-Jung Kim, Hye-Jin Ahn, Hyeweon Kang, Jaehui Park, Seul gi Oh, Saehae Choi, Won-Kyu Lee, Ho-Woo Nam
    The Korean Journal of Parasitology.2020; 58(3): 249.     CrossRef
  • Autophagy in the control and pathogenesis of parasitic infections
    George Ghartey-Kwansah, Frank Adu-Nti, Benjamin Aboagye, Amandus Ankobil, Edward Eyipe Essuman, Yeboah Kwaku Opoku, Samuel Abokyi, Emmanuel Kwasi Abu, Johnson Nyarko Boampong
    Cell & Bioscience.2020;[Epub]     CrossRef
  • Interplay Between Toxoplasma gondii, Autophagy, and Autophagy Proteins
    Carlos S. Subauste
    Frontiers in Cellular and Infection Microbiology.2019;[Epub]     CrossRef
  • The Interplay of Host Autophagy and Eukaryotic Pathogens
    Robert J. Evans, Varadharajan Sundaramurthy, Eva-Maria Frickel
    Frontiers in Cell and Developmental Biology.2018;[Epub]     CrossRef
  • A Systematic Review of In vitro and In vivo Activities of Anti-Toxoplasma Drugs and Compounds (2006–2016)
    Mahbobeh Montazeri, Mehdi Sharif, Shahabeddin Sarvi, Saeed Mehrzadi, Ehsan Ahmadpour, Ahmad Daryani
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • The Toxoplasma Parasitophorous Vacuole: An Evolving Host–Parasite Frontier
    Barbara Clough, Eva-Maria Frickel
    Trends in Parasitology.2017; 33(6): 473.     CrossRef
  • Activation of a Neospora caninum EGFR-Like Kinase Facilitates Intracellular Parasite Proliferation
    Xiaoxia Jin, Guojiang Li, Xichen Zhang, Pengtao Gong, Yanhui Yu, Jianhua Li
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • Suppressors for Human Epidermal Growth Factor Receptor 2/4 (HER2/4): A New Family of Anti-Toxoplasmic Agents in ARPE-19 Cells
    Yeong Hoon Kim, Lokraj Bhatt, Hye-Jin Ahn, Zhaoshou Yang, Won-Kyu Lee, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(5): 491.     CrossRef
  • Human toxoplasmosis–Searching for novel chemotherapeutics
    Magdalena Antczak, Katarzyna Dzitko, Henryka Długońska
    Biomedicine & Pharmacotherapy.2016; 82: 677.     CrossRef
  • Clinically Available Medicines Demonstrating Anti-Toxoplasma Activity
    Andrew J. Neville, Sydney J. Zach, Xiaofang Wang, Joshua J. Larson, Abigail K. Judge, Lisa A. Davis, Jonathan L. Vennerstrom, Paul H. Davis
    Antimicrobial Agents and Chemotherapy.2015; 59(12): 7161.     CrossRef
  • 9,857 View
  • 95 Download
  • 15 Web of Science
  • Crossref

Original Articles

High Expression of Water-Soluble Recombinant Antigenic Domains of Toxoplasma gondii Secretory Organelles
Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
Korean J Parasitol 2014;52(4):367-376.
Published online August 29, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.4.367

Recombinant antigenic proteins of Toxoplasma gondii are alternative source of antigens which are easily obtainable for serodiagnosis of toxoplasmosis. In this study, highly antigenic secretory organellar proteins, dense granular GRA2 and GRA3, rhoptrial ROP2, and micronemal MIC2, were analyzed by bioinformatics approach to express as water-soluble forms of antigenic domains. The transmembrane region and disorder tendency of 4 secretory proteins were predicted to clone the genes into pGEX-4T-1 vector. Recombinant plasmids were transformed into BL21 (DE3) pLysS E. coli, and GST fusion proteins were expressed with IPTG. As a result, GST fusion proteins with GRA225-105, GRA339-138, ROP2324-561, and MIC21-284 domains had respectively higher value of IgG avidity. The rGST-GRA225-105 and rGST-GRA339-138 were soluble, while rGST-ROP2324-561 and rGST-MIC21-284 were not. GRA231-71, intrinsically unstructured domain (IUD) of GRA2, was used as a linker to enhance the solubility. The rGST-GRA231-71-ROP2324-561, a chimeric protein, appeared to be soluble. Moreover, rGST-GRA231-71-MIC21-284 was also soluble and had higher IgG avidity comparing to rGST-MIC21-284. These 4 highly expressed and water-soluble recombinant antigenic proteins may be promising candidates to improve the serodiagnosis of toxoplasmosis in addition to the major surface antigen of SAG1.

Citations

Citations to this article as recorded by  Crossref logo
  • Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis
    Karolina Sołowińska, Lucyna Holec-Gąsior
    Microorganisms.2024; 12(8): 1731.     CrossRef
  • A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
    Macarena Sarli, María B. Novoa, Matilde N. Mazzucco, Marcelo L. Signorini, Ignacio E. Echaide, Susana T. de Echaide, María E. Primo, Paulo Lee Ho
    PLOS ONE.2020; 15(2): e0229301.     CrossRef
  • Development of a novel fusion protein with Anaplasma marginale and A. centrale MSP5 improved performance of Anaplasma antibody detection by cELISA in infected and vaccinated cattle
    María E. Primo, Carolina S. Thompson, Beatriz S. Valentini, Macarena Sarli, María B. Novoa, Atilio J. Mangold, Susana T. de Echaide, Ulrike Gertrud Munderloh
    PLOS ONE.2019; 14(1): e0211149.     CrossRef
  • TheToxoplasma gondiidense granule protein TgGRA3 interacts with host Golgi and dysregulates anterograde transport
    Maika S. Deffieu, Tchilabalo Dilezitoko Alayi, Christian Slomianny, Stanislas Tomavo
    Biology Open.2019;[Epub]     CrossRef
  • 9,680 View
  • 77 Download
  • 4 Web of Science
  • Crossref
Genotyping of Toxoplasma gondii from Rats (Rattus rattus) in Riyadh, Saudi Arabia
Maha H. Elamin
Korean J Parasitol 2014;52(3):257-261.
Published online June 26, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.3.257

Toxoplasma 3 main clonal lineages are designated as type I, II, and III; however, atypical and mixed genotypes were also reported. This study was conducted for detection of Toxoplasma gondii genotypes in rats (Rattus rattus) in Riyadh region, Saudi Arabia. PCR test on T. gondii B1 gene was conducted on ELISA IgM positive samples for confirmation of the infection. However, genetic analysis of the SAG2 locus was performed to determine T. gondii genotypes using PCR-RFLP technique. PCR test on T. gondii B1gene showed that 22 (81.5%) out of the 27 ELISA IgM positive samples have T. gondii DNA. Genotypic analysis shows that, of the total 22 PCR positive samples, only 13 (59.1%) were of type II, 7 (31.8%) were of type III, and 2 (9.1%) were of an unknown genotype. It is obvious that the prevalence of both type II and III is high in rats. No reports have been available on T. gondii genotypes among rats in Riyadh region, and only little is known about its seroprevalence in rats. Future studies on T. gondii genotypes in rats using multi-locus markers is needed in Riyadh region, Saudi Arabia for better understanding of T. gondii pathogenesis and treatment in humans and animals.

Citations

Citations to this article as recorded by  Crossref logo
  • Toxoplasma gondii infection affects the complete blood count and disturbs the markers of oxidative stress from the vital organs of wild rodents
    Maryam Ijaz, Asmat Ullah Khan, Shakir Ullah, Afshan Khan, Samir Ibenmoussa, Baye Sitotaw, Turki M. Dawoud, Adil Khan, Furhan Iqbal
    Scientific Reports.2024;[Epub]     CrossRef
  • Prevalence of Toxoplasma gondii infection in animals of the Arabian Peninsula between 2000–2020: A systematic review and meta‐analysis
    Asmaa Abdelgadier, Nada Assaad, Zaynab Elhussein, Abdulla M. Al‐Marri, Sami Suliman, Khalid Eltom, Ebtisam A. Al‐Mslemani, Abdul Azia Al‐Zeyara, Abdel Rahim M. El Hussein, Khalid A. Enan
    Veterinary Medicine and Science.2023; 9(1): 471.     CrossRef
  • The Detection of Toxoplasma gondii in Wild Rats (Rattus norvegicus) on Mink Farms in Shandong Province, Eastern China
    Yang Zou, Hong-Li Geng, Hong-Lin Jia, Quan Zhao, Si-Yuan Qin, Xing-Quan Zhu, Xiao-Xuan Zhang
    Vector-Borne and Zoonotic Diseases.2022; 22(3): 199.     CrossRef
  • Epidemiological Significance of Toxoplasma gondii Infections in Wild Rodents: 2009–2020
    J. P. Dubey, F. H. A. Murata, C. K. Cerqueira-Cézar, O. C. H. Kwok, C. Su
    Journal of Parasitology.2021;[Epub]     CrossRef
  • Molecular Detection and Genotyping of Toxoplasma gondii in Edward's Long-Tailed Rats (Leopoldamys edwardsi)
    Wen-Bin Zheng, Bin-Ze Gui, Hai-Bin Long, Yi-Wei Chen, Xing-Quan Zhu, Shui-Lian Wang, Guo-Hua Liu
    Foodborne Pathogens and Disease.2019; 16(8): 539.     CrossRef
  • 9,154 View
  • 97 Download
  • 9 Web of Science
  • Crossref
Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis
Khadijeh Khanaliha, Mohammad Hossein Motazedian, Bahram Kazemi, Bahador Shahriari, Mojgan Bandehpour, Zarin Sharifniya
Korean J Parasitol 2014;52(2):137-142.
Published online April 18, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.2.137

Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commercial ELISA. The recombinant plasmids were transformed to JM109 strain of Escherichia coli, and the recombinants were expressed and purified. Recombinant SAG1, SAG2, and SAG3 antigens were evaluated using different groups of sera in an ELISA system, and the results were compared to those of a commercial IgG and IgM ELISA kit. The sensitivity and specificity of recombinant surface antigens for detection of anti-Toxoplasma IgG in comparison with commercially available ELISA were as follows: SAG1 (93.6% and 92.9%), SAG2 (100.0% and 89.4%), and SAG3 (95.4% and 91.2%), respectively. A high degree of agreement (96.9%) was observed between recombinant SAG2 and commercial ELISA in terms of detecting IgG anti-Toxoplasma antibodies. P22 had the best performance in detecting anti-Toxoplasma IgM in comparison with the other 2 recombinant antigens. Recombinant SAG1, SAG2, and SAG3 could all be used for diagnosis of IgG-specific antibodies against T. gondii.

Citations

Citations to this article as recorded by  Crossref logo
  • IgM Antibody Detection as a Diagnostic Marker for Acute Toxoplasmosis: Current Status of Studies and Main Limitations
    Karolina Sołowińska, Lucyna Holec-Gąsior
    Antibodies.2025; 14(2): 44.     CrossRef
  • Analysis of the Correlation Between Toxoplasma gondii Seropositivity and Alzheimer’s Disease
    Jianjun Wang, Ping Lin, Dan Li, Biyu Yang, Jiaqi Wang, Meng Feng, Xunjia Cheng
    Pathogens.2024; 13(11): 1021.     CrossRef
  • Seroprevalence and Epidemiology of Toxoplasma gondii in Animals in the Qinghai-Tibetan Plateau Area, China
    Guojing Li, Wangli Zheng, Jinfang Yang, Tongsheng Qi, Yongcai He, Wangkai Chen, Hejia Ma, Yali Sun, Ying Li, Ming Kang, Jixu Li
    Pathogens.2021; 10(4): 432.     CrossRef
  • Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen
    Min-Ju Kim, Jie Mao, Hae-Ji Kang, Ki-Back Chu, Fu-Shi Quan
    The Korean Journal of Parasitology.2021; 59(6): 565.     CrossRef
  • Prevalence of Toxoplasma gondii parasite in captive Mexican jaguars determined by recombinant surface antigens (SAG1) and dense granular antigens (GRA1 and GRA7) in ELISA-based serodiagnosis
    Alejandro Reynoso-Palomar, Dulce Moreno-Gálvez, Abel Villa-Mancera
    Experimental Parasitology.2020; 208: 107791.     CrossRef
  • Evaluation of a PCR assay for diagnosis of toxoplasmosis in serum and peripheral blood mononuclear cell among HIV/AIDS patients
    Farah Bokharaei-Salim, Abdoulreza Esteghamati, Khadijeh Khanaliha, Saeed Kalantari, Shirin Sayyahfar, Tahereh Donyavi, Saba Garshasbi, Qasem Asgari, Borna Salemi
    Journal of Parasitic Diseases.2020; 44(1): 159.     CrossRef
  • Toxoplasma gondii seropositivity and serointensity and cognitive function in adults
    Shawn D. Gale, Lance D. Erickson, Evan L. Thacker, Elizabeth L. Mitchell, Bruce L. Brown, Dawson W. Hedges, Mathieu Nacher
    PLOS Neglected Tropical Diseases.2020; 14(10): e0008733.     CrossRef
  • Human toxoplasmosis in Mozambique: gaps in knowledge and research opportunities
    Leonardo Manuel, Gabriela Santos-Gomes, Emilia V. Noormahomed
    Parasites & Vectors.2020;[Epub]     CrossRef
  • The soluble fraction of Neospora caninum treated with PI-PLC is dominated by NcSRS29B and NcSRS29C
    Marcos Alexandre Bezerra, Luiz Miguel Pereira, Luciana Baroni, Ana Patrícia Yatsuda
    Experimental Parasitology.2019; 204: 107731.     CrossRef
  • Research Progress of Antigens Related to the Process of Toxoplasma gondii Invading Host Cell
    晓敬 孙
    Open Journal of Natural Science.2019; 07(06): 585.     CrossRef
  • Toxoplasma gondii-positive human sera recognise intracellular tachyzoites and bradyzoites with diverse patterns of immunoreactivity
    Marijo S. Roiko, Kaice LaFavers, Diane Leland, Gustavo Arrizabalaga
    International Journal for Parasitology.2018; 48(3-4): 225.     CrossRef
  • Development of an immunochromatographic test based on monoclonal antibodies against surface antigen 3 (TgSAG3) for rapid detection of Toxoplasma gondii
    Jiaqing Luo, Hongchao Sun, Xianfeng Zhao, Suhua Wang, Xunhui Zhuo, Yi Yang, Xueqiu Chen, Chaoqun Yao, Aifang Du
    Veterinary Parasitology.2018; 252: 52.     CrossRef
  • Antigens to Detect the Acute Phase of Toxoplasmosis in Pregnant Women: Standardized Comparison
    Juan Gabriel Costa, María Julia Vilariño
    Biomarkers in Medicine.2018; 12(5): 517.     CrossRef
  • P35 and P22 Toxoplasma gondii antigens abbreviate regions to diagnose acquired toxoplasmosis during pregnancy: toward single-sample assays
    Juan G. Costa, Leandro E. Peretti, Valeria S. García, Luz Peverengo, Verónica D.G. González, Luis M. Gugliotta, Maria L. Dalla Fontana, Claudia M. Lagier, Iván S. Marcipar
    Clinical Chemistry and Laboratory Medicine (CCLM).2017;[Epub]     CrossRef
  • Cloning and Sequencing of Truncated Toxoplasma gondii Subtilisin-Like 1 Antigen
    Ahmad Rouhizadeh, Ata A Ghadiri, Mohammad Razi Jalali, Masoud Ghorbanpour, Mohammad Hossein Razi Jalali
    Zahedan Journal of Research in Medical Sciences.2016;[Epub]     CrossRef
  • Evaluation of Recombinant SRS3 Antigen for Diagnosis of Toxoplasmosis by Enzyme-Linked Immunosorbent Assay
    Seyedeh Mahya Marashiyan, Fatemeh Moradian, Geita Saadatnia, Majid Golkar
    Archives of Clinical Infectious Diseases.2016;[Epub]     CrossRef
  • Cloning and Expression of Major Surface Antigen 1 Gene of Toxoplasma gondii RH Strain Using the Expression Vector pVAX1 in Chinese Hamster Ovary Cells
    Rahman Abdizadeh, Sharif Maraghi, Ata A. Ghadiri, Mehdi Tavalla, Saeedeh Shojaee
    Jundishapur Journal of Microbiology.2015;[Epub]     CrossRef
  • 11,479 View
  • 109 Download
  • 17 Web of Science
  • Crossref

Brief Communications

Seroprevalence of Toxoplasma gondii Infection in Police Dogs in Shenyang, Northeastern China
Cheng-Wu Liu, Na Yang, Jian-Bin He, Ming-Yang Mu, Min Yang, Ning Sun, Hong-Kui Li
Korean J Parasitol 2013;51(5):579-581.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.579

In recent years, worldwide surveys of Toxoplasma gondii infection in dogs have been reported. However, only limited surveys of T. gondii infection in police dogs have been available, including China. In the present study, we report the seroprevalence of T. gondii in police dogs in Shenyang, northeastern China. Sera from 291 police dogs were examined for T. gondii antibodies with the modified agglutination test (MAT), and 30.9% animals were tested seropositive. The results of the present study indicated a relatively high prevalence of T. gondii infection in police dogs in Shenyang, China.

Citations

Citations to this article as recorded by  Crossref logo
  • First Sero-Molecular Diagnosis of Toxoplasma gondii and Toxocara spp. Infections in the Police Dogs and Their Trainers in Iran
    Ali Asghari, Shirin Jalili, Nader Azadi
    Acta Parasitologica.2024; 69(3): 1724.     CrossRef
  • Investigation of Toxoplasma gondii Infection in Yunnan Semi-fine Wool Sheep (Ovis aries) and wild Rodents in Yunnan, China
    Zhao Li, Wen-Jie Cheng, Cai-Qin Deng, Meng-Ling Deng, Hai-Bo Peng, Xing-Quan Zhu, Feng-Cai Zou
    Acta Parasitologica.2024; 69(3): 1592.     CrossRef
  • Screening of Toxoplasmosis in Owned and Stray Dogs of District Faisalabad, Pakistan through Latex Agglutination and Indirect ELISA
    Hamidullah, Muhammad Saqib, Muhammad Sohail Sajid, Sabir Hussain, Hafiz Muhammad Rizwan, Khurram Ashfaq, Sadia Ghazanfer, Asif Ali Butt, Mahvish Maqbool, Sibtain Ahmad, Olivier Andre Sparagano
    Pathogens.2022; 11(11): 1307.     CrossRef
  • Toxoplasma gondii infections in dogs: 2009-2020
    Jitender P. Dubey, Fernando H.A. Murata, Camila K. Cerqueira-Cézar, Oliver C.H. Kwok, Yurong Yang, Chunlei Su
    Veterinary Parasitology.2020; 287: 109223.     CrossRef
  • Seroprevalence and Risk Factors of Toxoplasma gondii Infection in Farmed Raccoon Dogs (Nyctereutes procyonoides) in China
    Wen-Bin Zheng, Wei Cong, Junling Hou, Jian-Gang Ma, Xiao-Xuan Zhang, Xing-Quan Zhu, Qing-Feng Meng, Dong-Hui Zhou
    Vector-Borne and Zoonotic Diseases.2017; 17(3): 209.     CrossRef
  • Seroprevalence and risk factors of Toxoplasma gondii infection in stray dogs in northern China
    Xiao-Xuan Zhang, Ya-Nan Cai, Chun-Feng Wang, Jing Jiang, Ying-Tian Xu, Gui-Lian Yang, Quan Zhao
    Parasitology Research.2015; 114(12): 4725.     CrossRef
  • Low prevalence of Neospora caninum and Toxoplasma gondii antibodies in dogs in Jilin, Henan and Anhui Provinces of the People’s Republic of China
    Yurong Yang, Qiongfang Zhang, Yangguang Kong, Yuqing Ying, Oliver Hung Chun Kwok, Hongde Liang, Jitender Prakash Dubey
    BMC Veterinary Research.2014;[Epub]     CrossRef
  • Prevalence of Toxoplasma gondii antibodies in stray and owned dogs of Grenada, West Indies
    Ravindra N. Sharma, Gabriel Ordas, Keshaw Tiwari, Alfred Chikweto, Muhammad Iqbal Bhaiyat, Claude De Allie, Tara Paterson
    Veterinary World.2014; 7(9): 661.     CrossRef
  • Seroprevalence of Toxoplasma gondii infection in dogs and cats in Zhenjiang City, Eastern China
    Qing-Xin Liu, Shuai Wang, Li-Qun Wang, Jun Xing, Wen-Jue Gao, Guo-Fang Liu, Bin Zhao, Hai-Bin Zhang, Li-Hua Gao
    Asian Pacific Journal of Tropical Biomedicine.2014; 4(9): 725.     CrossRef
  • 9,710 View
  • 67 Download
  • Crossref
Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR
Yanhua Wang, Guangxiang Wang, Delin Zhang, Hong Yin, Meng Wang
Korean J Parasitol 2013;51(5):573-577.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.573

A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.

Citations

Citations to this article as recorded by  Crossref logo
  • First report of molecular detection and phylogenetic analysis of Toxoplasma Gondii in soil, water and vegetables from Chandigarh city, India
    Divya Rattan, Priya Datta, Devyani Sharma, Chandra Kanta Bhusal, Rakesh Sehgal
    Scientific Reports.2025;[Epub]     CrossRef
  • Detection of chronic toxoplasmosis in the brain of mice using loop-mediated isothermal amplification (LAMP) and conventional PCR
    Mona K. Hegazy, Nora E. Saleh, Wafaa A. Aboukamar
    Experimental Parasitology.2023; 251: 108556.     CrossRef
  • Immune system roles in pathogenesis, prognosis, control, and treatment of Toxoplasma gondii infection
    Mohammad Mahdi Jafari, Zahra Azimzadeh Tabrizi, Mohammad Saaid Dayer, Nazanin Atieh Kazemi-Sefat, Mahshid Mohtashamifard, Rahimeh Mohseni, Atefeh Bagheri, Saeed Bahadory, Amir Karimipour-Saryazdi, Fatemeh Ghaffarifar
    International Immunopharmacology.2023; 124: 110872.     CrossRef
  • Clinical validation of visual LAMP and qLAMP assays for the rapid detection of Toxoplasma gondii
    Zhi Cao, Ke Zhang, Dehua Yin, Qiaoya Zhang, Ying Yu, Jianxin Wen, Hongbo Ni
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • RAA-Cas12a-Tg: A Nucleic Acid Detection System for Toxoplasma gondii Based on CRISPR-Cas12a Combined with Recombinase-Aided Amplification (RAA)
    Qiao-Ni Ma, Meng Wang, Lai-Bao Zheng, Zi-Qin Lin, Muhammad Ehsan, Xing-Xing Xiao, Xing-Quan Zhu
    Microorganisms.2021; 9(8): 1644.     CrossRef
  • Loop mediated isothermal amplification (LAMP) of Toxoplasma DNA from dried blood spots
    Mona K. Hegazy, Soha I. Awad, Nora E. Saleh, Mamdouh M. Hegazy
    Experimental Parasitology.2020; 211: 107869.     CrossRef
  • Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection
    Ali Rostami, Panagiotis Karanis, Shirzad Fallahi
    Infection.2018; 46(3): 303.     CrossRef
  • Molecular detection and phylogenetic analyses ofToxoplasma gondiifrom naturally infected sheep in Northern and Central Tunisia
    Mariem Rouatbi, Yosra Amdouni, Safa Amairia, Mohamed R. Rjeibi, Said Sammoudi, Mourad Rekik, Mohamed Gharbi
    Veterinary Medicine and Science.2017; 3(1): 22.     CrossRef
  • Comparison of PCR assays targeting the multi-copy targets B1 gene and 529 bp repetitive element for detection of Toxoplasma gondii in swine muscle
    Fabrizia Veronesi, Azzurra Santoro, Giovanni Luigi Milardi, Manuela Diaferia, Raffaella Branciari, Dino Miraglia, Attilia Cioffi, Simona Gabrielli, David Ranucci
    Food Microbiology.2017; 63: 213.     CrossRef
  • Molecular detection of Toxoplasma gondii in house sparrow (Passer domesticus) by LAMP and PCR methods in Tehran, Iran
    Amir Abdoli, Abdolhossein Dalimi, Haleh Soltanghoraee, Fatemeh Ghaffarifar
    Journal of Parasitic Diseases.2016; 40(4): 1317.     CrossRef
  • Development and application of loop-mediated isothermal amplification assays based on ITS-1 for rapid detection of Toxoplasma gondii in pork
    Xunhui Zhuo, Bin Huang, Jiaqing Luo, Haijie Yu, Baolong Yan, Yi Yang, Aifang Du
    Veterinary Parasitology.2015; 208(3-4): 246.     CrossRef
  • Diagnosis of toxoplasmosis and typing of Toxoplasma gondii
    Quan Liu, Ze-Dong Wang, Si-Yang Huang, Xing-Quan Zhu
    Parasites & Vectors.2015;[Epub]     CrossRef
  • Loop-Mediated Isothermal Amplification for Rapid and Semiquantitative Detection of Loa loa Infection
    Papa M. Drame, Doran L. Fink, Joseph Kamgno, Jesica A. Herrick, Thomas B. Nutman, M. J. Loeffelholz
    Journal of Clinical Microbiology.2014; 52(6): 2071.     CrossRef
  • 7,576 View
  • 94 Download
  • Crossref

Original Article

A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
Kyoung Ju Song, Zhaoshou Yang, Chom-Kyu Chong, Jin-Soo Kim, Kyung Chan Lee, Tong-Soo Kim, Ho-Woo Nam
Korean J Parasitol 2013;51(5):503-510.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.503

Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.

Citations

Citations to this article as recorded by  Crossref logo
  • Development of an Immunochromatographic Test with Recombinant MIC2-MIC3 Fusion Protein for Serological Detection of Toxoplasma gondii
    Jianzhong Wang, Yi Zhao, Jicheng Qiu, Jing Liu, Rui Zhou, Xialin Ma, Xiaojie Wu, Xiaoguang Li, Wei Mao, Yiduo Liu, Heng Zhang
    Veterinary Sciences.2025; 12(6): 509.     CrossRef
  • Application of gold immunochromatographic assay strip combined with digital evaluation for early detection of Toxoplasma gondii infection in multiple species
    Jiyuan Fan, Hao Sun, Jiawen Fang, Yafan Gao, Haojie Ding, Bin Zheng, Qingming Kong, Xunhui Zhuo, Shaohong Lu
    Parasites & Vectors.2024;[Epub]     CrossRef
  • Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis
    Karolina Sołowińska, Lucyna Holec-Gąsior
    Microorganisms.2024; 12(8): 1731.     CrossRef
  • Seroprevalence of Toxoplasma gondii infection among patients of a tertiary hospital in Guangzhou, Guangdong province, PR China
    Yu-bin Guan, Xiao-xiao Sun, Shao-lian Chen, Xiao-ting Zhu, Zhi-hua Zeng, Han-wei Lu, Hong-mei Feng, Yu Guo, Wen-gong Jiang, Kui Xiong, Xiao-rong Yang, Ho-Woo Nam, Zhao-shou Yang, Pan Li
    PLOS ONE.2023; 18(7): e0286430.     CrossRef
  • Past and present seroprevalence and disease burden estimates of Toxoplasma gondii infections in Germany: An appreciation of the role of serodiagnostics
    Frank Seeber
    International Journal of Medical Microbiology.2023; 313(6): 151592.     CrossRef
  • Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea
    Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(1): 9.     CrossRef
  • Clusters of Toxoplasmosis in Ganghwa-gun, Cheorwon-gun, and Goseong-gun, Korea
    Jihye Yu, Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(3): 251.     CrossRef
  • Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals
    Amjad Hayat Khan, Rahmah Noordin
    European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(1): 19.     CrossRef
  • Clusters of Toxoplasmosis in Gyodong-Myeon and Samsan-Myeon, Ganghwa-Gun, Korea
    Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2020; 58(5): 493.     CrossRef
  • Identification of novel antigens for serum IgG diagnosis of human toxoplasmosis
    Juntao Luo, Jingyi Wan, Ziru Tang, Shuang Shen
    Experimental Parasitology.2019; 204: 107722.     CrossRef
  • Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up
    Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(3): 247.     CrossRef
  • Development of direct assays for Toxoplasma gondii and its use in genomic DNA sample
    Lívia M. Alves, Vinícius R. Rodovalho, Ana C.H. Castro, Márcia A.R. Freitas, Caroline M. Mota, Tiago W.P. Mineo, José R. Mineo, João M. Madurro, Ana G. Brito-Madurro
    Journal of Pharmaceutical and Biomedical Analysis.2017; 145: 838.     CrossRef
  • Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea
    Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(4): 385.     CrossRef
  • Western Blot Detection of Human Anti-Chikungunya Virus Antibody with Recombinant Envelope 2 Protein
    Zhaoshou Yang, Jihoo Lee, Hye-Jin Ahn, Chom-Kyu Chong, Ronaldo F. Dias, Ho-Woo Nam
    The Korean Journal of Parasitology.2016; 54(2): 239.     CrossRef
  • Digested disorder, Quarterly intrinsic disorder digest (October-November-December, 2013)
    Shelly DeForte, Krishna D Reddy, Vladimir N Uversky
    Intrinsically Disordered Proteins.2015; 3(1): e984569.     CrossRef
  • Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients
    Ho-Woo Nam, Kyoung Ju Song, Hye Jin Ahn, Zhaoshou Yang, Chom-Kyu Chong, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim
    The Korean Journal of Parasitology.2014; 52(2): 143.     CrossRef
  • High Expression of Water-Soluble Recombinant Antigenic Domains ofToxoplasma gondii Secretory Organelles
    Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2014; 52(4): 367.     CrossRef
  • 9,682 View
  • 106 Download
  • Crossref

Brief Communication

IgG Western Blot for Confirmatory Diagnosis of Equivocal Cases of Toxoplasmosis by EIA-IgG and Fluorescent Antibody Test
Imen Khammari, Fatma Saghrouni, Alia Yaacoub, Sondoss Gaied Meksi, Hinda Ach, Lamia Garma, Akila Fathallah, Moncef Ben Sa?d
Korean J Parasitol 2013;51(4):485-488.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.485

The performance values of available techniques used in serodiagnosis of toxoplasmosis are satisfactory but they raise problems of equivocal and discordant results for very low IgG titers. Recently marketed, LDBio-Toxo II IgG Western blot (IB) showed an excellent correlation with the dye test. We estimated the proportion of equivocal and discordant results between the enzyme immunoassay Platelia Toxo IgG (EIA-IgG) and fluorescent antibody test (FAT) and assessed the usefulness of the IB as a confirmatory test. Out of 2,136 sera collected from pregnant women, 1,644 (77.0%) tested unequivocally positive and 407 (19.0%) were negative in both EIA-IgG and FAT. The remaining 85 (4%) sera showed equivocal or discordant results. Among them, 73 (85.9%) were positive and 12 (14.1%) were negative in IB. Forty-one (89.1%) equivocal sera in EIA-IgG and 46 (86.8%) equivocal sera in FAT were positive in IB. Reducing the cut-off values of both screening techniques improved significantly their sensitivity in detecting very low IgG titers at the expense of their specificity. In conclusion, equivocal results in routine-used techniques and their discordance in determination of the immune status in pregnancy women were not uncommon. IB test appeard to be highly useful in these situations as a confirmatory technique.

Citations

Citations to this article as recorded by  Crossref logo
  • Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges
    Priscila Silva Franco, Ana Carolina Morais Oliveira Scussel, Rafaela José Silva, Thadia Evelyn Araújo, Henrique Tomaz Gonzaga, Camila Ferreira Marcon, Joaquim Pedro Brito-de-Sousa, Angélica Lemos Debs Diniz, Marina Carvalho Paschoini, Bellisa Freitas Barb
    Journal of Tropical Medicine.2024; 2024: 1.     CrossRef
  • Congenital Toxoplasmosis Diagnosis: Current Approaches and New Insights
    Alfredo Márquez-Mauricio, Heriberto Caballero-Ortega, Fernando Gómez-Chávez
    Acta Parasitologica.2023; 68(3): 473.     CrossRef
  • Performances of ICT Toxoplasma IgG-IgM test in comparison with Vidas® toxo competition to determine the immune status against Toxoplasma gondii
    Sylvie Abraham, Raphael Piarroux, Ying Zhou, Vera Tesic, Ana Abeleda, Nadhira Houhou-Fidouh, Pascale Nicaise-Rolland, Luce Landraud, Rima McLeod, Sandrine Houzé
    European Journal of Clinical Microbiology & Infectious Diseases.2023; 42(11): 1327.     CrossRef
  • Determination of an optimal ELISA cut-off for the diagnosis of Toxoplasma gondii infection in pigs using Bayesian latent class modelling of data from multiple diagnostic tests
    Abbey Olsen, Henrik Vedel Nielsen, Lis Alban, Hans Houe, Tina Birk Jensen, Matthew Denwood
    Preventive Veterinary Medicine.2022; 201: 105606.     CrossRef
  • Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
    Valeria Meroni, Francesca Genco, Luigia Scudeller, Marie-Pierre Brenier-Pinchart, Hélène Fricker-Hidalgo, Coralie L’Ollivier, Luc Paris, Hervé Pelloux
    Pathogens.2022; 11(6): 665.     CrossRef
  • Difficultés d’interprétation de la sérologie toxoplasmose
    Luc Paris, Sandrine Houzé
    Revue Francophone des Laboratoires.2022; 2022(545): 33.     CrossRef
  • Serological diagnosis of toxoplasmosis: evaluation of the commercial test recomLine Toxoplasma IgG immunoblot (Mikrogen) based on recombinant antigens
    Vincent Jean-Pierre, Julien Miozzo, Hélène Fricker-Hidalgo, Cécile Garnaud, Marie Gladys Robert, Hervé Pelloux, Marie-Pierre Brenier-Pinchart
    Parasite.2022; 29: 52.     CrossRef
  • Contribution of the Toxoplasma ICT IgG IgM® test in determining the immune status of pregnant women against toxoplasmosis
    Ben‐Abdallah Rym, Kalboussi Yasmine, Bellali Hedia, Issaoui Nesrine, Souissi Olfa, Maatoug Rania, Aoun Karim, Bouratbine Aïda
    Journal of Clinical Laboratory Analysis.2021;[Epub]     CrossRef
  • A longitudinal study of Toxoplasma gondii seroconversion on four large Danish sow farms
    Abbey Olsen, Lis Alban, Matthew Denwood, Hans Houe, Tina Birk Jensen, Henrik Vedel Nielsen
    Veterinary Parasitology.2021; 295: 109460.     CrossRef
  • Contribution of the "Recomline Toxoplasma IgM" Kit in the Distinction between Toxoplasmic IgM and Natural IgM
    Khammari Imen, Ben Halima Nada, Ismaïl Samar, Chouaieb Hamed, Fathallah Akila
    International Journal Of Pharmaceutical And Phytopharmacological Research.2021; 11(6): 15.     CrossRef
  • Evaluating the “Recomline Toxoplasma IgM” Kit for Differentiating Toxoplasmic and Natural IgM
    Imen Khammari, Nada Ben Halima, Samar Ismaïl, Hamed Chouaieb, Akila Fathallah
    Interdisciplinary Research in Medical Sciences Specialty.2021; 1(2): 1.     CrossRef
  • Performance of a Toxo IgM prototype assay for the diagnosis of maternal and congenital Toxoplasma infections
    Martine Wallon, Hélène Fricker-Hidalgo, Emmanuelle Chapey, Claire Bailet, Céline Dard, Marie-Pierre Brenier-Pinchart, Hervé Pelloux
    Clinical Chemistry and Laboratory Medicine (CCLM).2020; 58(11): 1959.     CrossRef
  • Performance of Zika Assays in the Context of Toxoplasma gondii, Parvovirus B19, Rubella Virus, and Cytomegalovirus (TORCH) Diagnostic Assays
    Bettie Voordouw, Barry Rockx, Thomas Jaenisch, Pieter Fraaij, Philippe Mayaud, Ann Vossen, Marion Koopmans
    Clinical Microbiology Reviews.2019;[Epub]     CrossRef
  • Establishing tools for early diagnosis of congenital toxoplasmosis: Flow cytometric IgG avidity assay as a confirmatory test for neonatal screening
    Aline de Castro Zacche-Tonini, Giuliana Schmidt França Fonseca, Laura Néspoli Nassar Pansini de Jesus, Geisa Baptista Barros, Jordana Grazziela Alves Coelho-dos-Reis, Samantha Ribeiro Béla, Anderson Silva Machado, Ana Carolina Aguiar Vasconcelos Carneiro,
    Journal of Immunological Methods.2017; 451: 37.     CrossRef
  • A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women
    Imen Khammari, Fatma Saghrouni, Sami Lakhal, Aida Bouratbine, Moncef Ben Said, Jalel Boukadida
    The Korean Journal of Parasitology.2014; 52(5): 493.     CrossRef
  • Identification of soluble and membrane antigenic markers of acquired toxoplasmosis by immunoblot
    I. Khammari, F. Saghrouni, S. Lakhal, I. Bougmiza, A. Bouratbine, M. Ben Said, J. Boukadida
    Parasite Immunology.2014; 36(12): 684.     CrossRef
  • 8,762 View
  • 98 Download
  • Crossref

Original Article

Influence of Toxoplasma gondii Acute Infection on Cholinesterase Activities of Wistar Rats
Alexandre Alberto Tonin, Aleksandro Schafer da Silva, Maria Luiza Thorstenberg, L?via Gelain Castilhos, Raqueli Teresinha Fran?a, Daniela Bitencourt Rosa Leal, Marta Maria Medeiros Frescura Duarte, Fernanda Silveira Flores Vogel, Mario Luiz de La Rue, Sonia Terezinha dos Anjos Lopes
Korean J Parasitol 2013;51(4):421-426.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.421

Several studies have shown the mechanisms and importance of immune responses against Toxoplasma gondii infection and the notable role of cholinesterases in inflammatory reactions. However, the association between those factors has not yet been investigated. Therefore, the aim of this study was to evaluate the acetylcholinesterase (AChE) activity in blood and lymphocytes and the activity of butyrylcholinesterase (BChE) in serum of rats experimentally infected with T. gondii during the acute phase of infection. For that, an in vivo study was performed with evaluations of AChE and BChE activities on days 5 and 10 post-infection (PI). The activity of AChE in blood was increased on day 5 PI, while in lymphocytes its activity was enhanced on days 5 and 10 PI (P<0.05). No significant difference was observed between groups regarding to the activity of BChE in serum. A positive (P<0.01) correlation was observed between AChE activity and number of lymphocytes. The role of AChE as an inflammatory marker is well known in different pathologies; thus, our results lead to the hypothesis that AChE has an important role in modulation of early immune responses against T. gondii infection.

Citations

Citations to this article as recorded by  Crossref logo
  • A meta-analysis of changes in cholinesterase activity among experimental animals infected with Toxoplasma gondii
    F. K. Mohammad, S. H. Eassa, R. G. Abdullah
    BULGARIAN JOURNAL OF VETERINARY MEDICINE.2025; 28(2): 195.     CrossRef
  • Variation in Gene Expression Across Infection Status and Elevation in a Hawaiian Honeycreeper
    Loren Cassin‐Sackett, Katherine M. McClure, Taylor E. Callicrate, Eben H. Paxton, Robert C. Fleischer
    Ecology and Evolution.2025;[Epub]     CrossRef
  • Plasma Cholinesterase Activity in Patients With Rheumatoid Arthritis and Toxoplasmosis
    Rabie G Abdullah, Souzan H Eassa, Fouad K Mohammad
    Cureus.2023;[Epub]     CrossRef
  • Toxoplasmosis: Targeting neurotransmitter systems in psychiatric disorders
    Tooran Nayeri, Shahabeddin Sarvi, Ahmad Daryani
    Metabolic Brain Disease.2022; 37(1): 123.     CrossRef
  • Toxoplasma gondii: A possible etiologic agent for Alzheimer's disease
    Tooran Nayeri, Shahabeddin Sarvi, Mehdi Sharif, Ahmad Daryani
    Heliyon.2021; 7(6): e07151.     CrossRef
  • Diagnostic significance of C-reactive protein and hematological parameters in acute toxoplasmosis
    Vanessa Sandri, Itamar Luís Gonçalves, Gustavo Machado das Neves, Marisa Lucia Romani Paraboni
    Journal of Parasitic Diseases.2020; 44(4): 785.     CrossRef
  • STUDY ON THE PREVALENCE AND HEMATOLOGICAL ALTERATIONS IN TOXOPLASMA GONDII INFECTED CAPTIVE PHEASANT SPECIES OF BAHAWALPUR ZOO, PAKISTAN
    M.H. Lashari, M Bibi, U Farooq, F Afzal, A Ali, M Safdar, M.S. Akhtar, A.A. Farooq, S Masood, M Ayaz, M.I. Khan
    The Journal of Animal and Plant Sciences.2020; 31(2): 625.     CrossRef
  • Is There a Cholinergic Survival Incentive for Neurotropic Parasites in the Brain?
    Abdul Mannan Baig
    ACS Chemical Neuroscience.2017; 8(12): 2574.     CrossRef
  • Sulfamethoxazole-trimethoprim associated with resveratrol for the treatment of toxoplasmosis in mice: Influence on the activity of enzymes involved in brain neurotransmission
    Nathieli B. Bottari, Matheus D. Baldissera, Alexandre A. Tonin, Virginia C. Rech, Vivian S.K. Nishihira, Gustavo R. Thomé, Maria Rosa C. Schetinger, Vera M. Morsch, Giovana Camillo, Fernanda F. Vogel, Camila Tochetto, Rafael Fighera, Gustavo Machado, Leni
    Microbial Pathogenesis.2015; 79: 17.     CrossRef
  • 8,766 View
  • 70 Download
  • Crossref

Mini Review

Clinical Features and Treatment of Ocular Toxoplasmosis
Young-Hoon Park, Ho-Woo Nam
Korean J Parasitol 2013;51(4):393-399.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.393

Ocular toxoplasmosis is a disease caused by the infection with Toxoplasma gondii through congenital or acquired routes. Once the parasite reaches the retina, it proliferates within host cells followed by rupture of the host cells and invasion into neighboring cells to make primary lesions. Sometimes the restricted parasite by the host immunity in the first scar is activated to infect another lesion nearby the scar. Blurred vision is the main complaint of ocular toxoplasmic patients and can be diagnosed by detection of antibodies or parasite DNA. Ocular toxoplasmosis needs therapy with several combinations of drugs to eliminate the parasite and accompanying inflammation; if not treated it sometimes leads to loss of vision. We describe here clinical features and currently available chemotherapy of ocular toxoplasmosis.

Citations

Citations to this article as recorded by  Crossref logo
  • Systematic screening identifies medication and disease factors associated with schizophrenia risk
    Ariel Israel, Abraham Weizman, Sarah Israel, Joshua Stokar, Shai Ashkenazi, Shlomo Vinker, Eli Magen, Eugene Merzon
    Brain, Behavior, and Immunity.2026; 131: 106135.     CrossRef
  • The importance of translational research in the study of ocular toxoplasmosis: insights from the 17th International Congress on Toxoplasmosis 2024
    Alejandra de-la-Torre, Germán Mejía-Salgado, Gereon Schares
    FEMS Microbes.2025;[Epub]     CrossRef
  • A COMBINATION REGIMEN OF INTRAVITREAL CLINDAMYCIN WITH DEXAMETHASONE, SYSTEMIC CO-TRIMOXAZOLE AND STEROIDS FOR MACULAR TOXOPLASMA RETINOCHOROIDITIS IN IMMUNOCOMPETENT CASES
    Sushree Satavasia, Akshay Mishra, Vishal Jadhav, Talluri Ronnie Abhishek, Anup Kelgaonkar, Anamika Patel, Soumyava Basu, Avinash Pathengay
    Retina.2025; 45(5): 871.     CrossRef
  • Deep Learning-Based Classification of Ocular Toxoplasmosis Fundus Images: A Comparative Study of CNN and SVM Models
    Shivam Tiwari, Kalicharan Sahu, Surbhi Vijh, Charu Awasthi
    Procedia Computer Science.2025; 259: 1189.     CrossRef
  • Epidemiological spectrum of infectious uveitis in the Asia-Pacific
    Alexander Newman, Peter McCluskey
    Taiwan Journal of Ophthalmology.2025; 15(2): 157.     CrossRef
  • Toxoplasmosis in patient underwent stem cell transplantation, bone marrow transplantation due to multiple myeloma
    Magdalena Bulek, Aleksandra Kopyś-Wócik, Natalie Papachristoforou, Aleksandra Kocjan
    Postępy Higieny i Medycyny Doświadczalnej.2025; 79(1): 106.     CrossRef
  • Toxoplasma gondii at the Host Interface: Immune Modulation and Translational Strategies for Infection Control
    Billy J. Erazo Flores, Laura J. Knoll
    Vaccines.2025; 13(8): 819.     CrossRef
  • A Comprehensive Review of Toxoplasmosis: Serious Threat to Human Health
    Aswin Rafif Khairullah, Shendy Canadya Kurniawan, Agus Widodo, Mustofa Helmi Effendi, Abdullah Hasib, Otto Sahat Martua Silaen, Sancaka Chasyer Ramandinianto, Ikechukwu Benjamin Moses, Katty Hendriana Priscilia Riwu, Sheila Marty Yanestria, Muhammad Esa E
    The Open Public Health Journal.2024;[Epub]     CrossRef
  • Benchmarking Deep Learning Frameworks for Automated Diagnosis of Ocular Toxoplasmosis: A Comprehensive Approach to Classification and Segmentation
    Syed Samiul Alam, Samiul Based Shuvo, Shams Nafisa Ali, Fardeen Ahmed, Arbil Chakma, Yeong Min Jang
    IEEE Access.2024; 12: 22759.     CrossRef
  • From Misdiagnosis to Management: A Case Report of Ocular Inflammatory Disease
    Catarina M Francisco, Miguel Santos, Sofia Mano, Inês Leal, Joana Coelho
    Cureus.2024;[Epub]     CrossRef
  • Posterior segment optical coherence tomography: A diagnostic aid in posterior uveitis
    Parul M. Danayak, Rupal M. Chaudhary, Zalak Shah
    Kerala Journal of Ophthalmology.2024; 36(2): 152.     CrossRef
  • Human Ocular Toxoplasmosis in Romania: History, Epidemiology, and Public Health: A Narrative Review
    Laura Andreea Ghenciu, Ovidiu Alin Hațegan, Sorin Lucian Bolintineanu, Alexandra-Ioana Dănilă, Roxana Iacob, Emil Robert Stoicescu, Maria Alina Lupu, Tudor Rareș Olariu
    Microorganisms.2024; 12(8): 1541.     CrossRef
  • Ocular Toxoplasmosis
    Eunice Jin Hui Goh, Ikhwanuliman Putera, Rina La Distia Nora, Padmamalini Mahendradas, Jyotirmay Biswas, Soon-Phaik Chee, Ilaria Testi, Carlos E Pavesio, Andre Luiz Land Curi, Daniel Vitor Vasconcelos-Santos, Atul Arora, Vishali Gupta, Alejandra de-la-Tor
    Ocular Immunology and Inflammation.2023; 31(7): 1342.     CrossRef
  • Etiology of Pediatric Uveitis in a Tertiary Pediatric Eye Hospital in Egypt
    Eiman Abd El Latif, Rasha Mousa, Mohammed Tawfeeq Mahdi, Ahmed Mahmoud Amin, Mahmoud Mohammed Ahmed Ali, Nour Eldin Abdelhamid, Mohamed Elmoddather, Hassan Shamselden Yousef, Ehab Hafez Gouda Hafez, Sameh Galal Taher Salem, Ashraf Hassan Soliman
    Ocular Immunology and Inflammation.2023; 31(10): 1978.     CrossRef
  • Interferon-gamma release assay and chest X-ray to classify intraocular tuberculosis among clinically undifferentiated uveitis
    Mei Riasanti, Ikhwanuliman Putera, Priscilla Jessica, Muhammad Zakiy Waliyuddin, Faiz Alwan Tagar, Andini Karlina CH, Yulia Aziza, Made Susiyanti, Lukman Edwar, Ratna Sitompul, Rina La Distia Nora
    Medical Journal of Indonesia.2023; 31(4): 225.     CrossRef
  • Stepwise approach for fundus imaging in the diagnosis and management of posterior uveitis
    Aniruddha Agarwal, Francesco Pichi, Alessandro Invernizzi, Dilraj S. Grewal, Rohan Bir Singh, Awaneesh Upadhyay
    Survey of Ophthalmology.2023; 68(3): 446.     CrossRef
  • Macular Pucker: A Devastating Complication in Ocular Toxoplasmosis
    Syarifah Nur Humaira Syed Mohd Khomsah, Julieana Muhammed, Wan-Hazabbah Wan Hitam
    Cureus.2023;[Epub]     CrossRef
  • Optical Coherence Tomography Angiography of the Choriocapillaris in Subclinical Toxoplasma Chorioretinitis: A Case Report
    Ahmed B Alsatrawi
    Cureus.2023;[Epub]     CrossRef
  • Retinal ferroptosis as a critical mechanism for the induction of retinochoroiditis during ocular toxoplasmosis
    Kazuhisa Yamada, Akira Tazaki, Nanako Ushio-Watanabe, Yoshihiko Usui, Atsunobu Takeda, Masaaki Matsunaga, Ayana Suzumura, Hideyuki Shimizu, Hao Zheng, Nanang R. Ariefta, Masahiro Yamamoto, Hideaki Hara, Hiroshi Goto, Koh-Hei Sonoda, Koji M. Nishiguchi, Ma
    Redox Biology.2023; 67: 102890.     CrossRef
  • Pediatric Posterior Infectious Uveitis
    André Luiz Land Curi, Alejandra de-la-Torre, Ariel Schlaen, Papdmamalini Mahendradas, Jyortimay Biswas
    Ocular Immunology and Inflammation.2023; 31(10): 1944.     CrossRef
  • Toxoplasmosis Related Neuroretinitis: More than Meets the Eye
    Alastair Coulson, Divya Jacob, Lyudmila Kishikova, Ahmed Saad
    Ocular Immunology and Inflammation.2022; 30(3): 556.     CrossRef
  • Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene
    Bahman Rahimi Esboei, Shirzad Fallahi, Mohammad Zarei, Bahram Kazemi, Mehdi Mohebali, Saeedeh Shojaee, Parisa Mousavi, Aref Teimouri, Raziyeh Mahmoudzadeh, Mirataollah Salabati, Hossein Keshavarz Valian
    BMC Infectious Diseases.2022;[Epub]     CrossRef
  • Toxoplasma Chorioretinitis
    Somya Ish, Shreyash Agrawal, Pranav Ish
    Indian Journal of Medical Specialities.2022; 13(1): 67.     CrossRef
  • Ocular Toxoplasmosis: A Review of Current Literature
    Neal S. Patel, Demetrios G. Vavvas
    International Ophthalmology Clinics.2022; 62(2): 231.     CrossRef
  • Calomys callosus: An Experimental Animal Model Applied to Parasitic Diseases Investigations of Public Health Concern
    Rafael Borges Rosa, Mylla Spirandelli da Costa, Samuel Cota Teixeira, Emilene Ferreira de Castro, Willyenne Marília Dantas, Eloisa Amália Vieira Ferro, Murilo Vieira da Silva
    Pathogens.2022; 11(3): 369.     CrossRef
  • Insights From the Eye for Patients With Kidney Transplant
    Christine Xu, Alisa J. Prager, Carolyn D. Alonso, Aditya S. Pawar
    Transplantation Proceedings.2022; 54(8): 2182.     CrossRef
  • An illustration of ocular toxoplasmosis with consequent lenticular opacities and asteroid hyalosis
    Dimakatso G. Mashala
    African Vision and Eye Health.2022;[Epub]     CrossRef
  • Azithromycin concentration in rabbits’ plasma and posterior segment of the eyes following oral drug administration: a dose-finding study for the application in ocular toxoplasmosis
    Hisar Daniel, Melva Louisa, Lukman Edwar
    Journal of Pharmacy and Pharmacology.2022; 74(12): 1743.     CrossRef
  • Revenge of the Tick: Tick-Borne Diseases and the Eye in the Age of Climate Change and Globalisation
    Xin Le Ng, Berdjette Y. Y. Lau, Cassandra X. C. Chan, Dawn K. A. Lim, Blanche X. H. Lim, Chris H. L. Lim
    Zoonotic Diseases.2022; 2(4): 183.     CrossRef
  • Infectious Sclerouveitis in an Immunocompetent Patient: A Probable Case of Simultaneous Ocular Tuberculosis and Toxoplasmosis
    Wen K Chong, Karen Khoo Kah Kuen, Lam Mun-Wei, Abdul-Salim Ismail, Azhany Yaakub
    Cureus.2022;[Epub]     CrossRef
  • Insights from the Eye for Patients with Kidney Transplant
    Christine Xu, Alisa J. Prager, Carolyn D. Alonso, Aditya Pawar
    SSRN Electronic Journal.2022;[Epub]     CrossRef
  • Ocular toxoplasmosis: phenotype differences between toxoplasma IgM positive and IgM negative patients in a large cohort
    Sofia Ajamil-Rodanes, Joshua Luis, Rabia Bourkiza, Benedict Girling, Angela Rees, Catherine Cosgrove, Carlos Pavesio, Mark Westcott
    British Journal of Ophthalmology.2021; 105(2): 210.     CrossRef
  • Retinal vasculitis: A framework and proposal for a classification system
    Ghazala A. Datoo O'Keefe, Narsing Rao
    Survey of Ophthalmology.2021; 66(1): 54.     CrossRef
  • Retinocoroiditis toxoplásmica de presentación atípica en pacientes con enfermedades hematológicas malignas
    J.P. Figueroa-Vercellino, L. Miguel, A. Moll-Udina, C. Alba-Linero, V. Llorenç, A. Adán
    Archivos de la Sociedad Española de Oftalmología.2021; 96(3): 152.     CrossRef
  • Atypical toxoplasmic retinochoroiditis in patients with malignant hematological diseases
    J.P. Figueroa-Vercellino, L. Miguel, A. Moll-Udina, C. Alba-Linero, V. Llorenç, A. Adán
    Archivos de la Sociedad Española de Oftalmología (English Edition).2021; 96(3): 152.     CrossRef
  • Detection of antibodies to Toxoplasma gondii among owned dogs in Cambodia
    Thi Thuy Nguyen, Chanya Kengradomkij, Tawin Inpankaew
    Food and Waterborne Parasitology.2021; 22: e00103.     CrossRef
  • Etiology of Uveitis in Upper Egypt
    Eiman Abd El Latif, Asaad Nooreldin, Mohammed Shikhoun Ahmed, Mohamed Elmoddather, Wael El Gendy
    Clinical Ophthalmology.2021; Volume 15: 195.     CrossRef
  • Screening for common eye diseases in the elderly with Optos ultra-wide-field scanning laser ophthalmoscopy: a pilot study with focus on ocular toxoplasmosis
    Pablo Eduardo Logroño Wiese, Frank Seeber, Anne-Sophie Endres, Claudia Brockmann, Uwe Pleyer
    International Ophthalmology.2021; 41(5): 1573.     CrossRef
  • Ocular Toxoplasmosis: Mechanisms of Retinal Infection and Experimental Models
    Veronica Rodriguez Fernandez, Giovanni Casini, Fabrizio Bruschi
    Parasitologia.2021; 1(2): 50.     CrossRef
  • Acquired Immunodeficiency Syndrome Presented as Atypical Ocular Toxoplasmosis
    Elias Khalili Pour, Hamid Riazi-Esfahani, Nazanin Ebrahimiadib, Violet Zaker Esteghamati, Mohammad Zarei, Kamal Kishore
    Case Reports in Ophthalmological Medicine.2021; 2021: 1.     CrossRef
  • The challenges of treating a patient with recurrent congenital toxoplasmic chorioretinitis
    Aleksandra Krasińska, Kamila Jaz, Joanna Mamczur, Jarosław Kocięcki
    Medical Journal of Cell Biology.2021; 9(2): 56.     CrossRef
  • A Trust-Based Methodology to Evaluate Deep Learning Models for Automatic Diagnosis of Ocular Toxoplasmosis from Fundus Images
    Rodrigo Parra, Verena Ojeda, Jose Luis Vázquez Noguera, Miguel García-Torres, Julio César Mello-Román, Cynthia Villalba, Jacques Facon, Federico Divina, Olivia Cardozo, Verónica Elisa Castillo, Ingrid Castro Matto
    Diagnostics.2021; 11(11): 1951.     CrossRef
  • The pathogenicity and virulence of Toxoplasma gondii
    Syrian G. Sanchez, Sébastien Besteiro
    Virulence.2021; 12(1): 3095.     CrossRef
  • Toxoplasma Retinochoroiditis with Chorioretinal Neovascularization in a Young Patient
    Nikhila S. Khandwala, Robert A. Hyde, Cagri G. Besirli
    Case Reports in Ophthalmology.2021; 12(1): 259.     CrossRef
  • The Diagnostic Conundrum of Retinitis and a Pigmented Scar
    Nikhila S. Khandwala, Jason M.L. Miller, Robert A. Hyde, Christopher D. Conrady, Rajesh C. Rao, Cagri G. Besirli
    Case Reports in Ophthalmology.2021; 12(1): 164.     CrossRef
  • OCULAR TOXOPLASMOSIS
    Iskren Kaftandjiev, Rumen Harizanov
    PROBLEMS of Infectious and Parasitic Diseases.2021; 49(1): 41.     CrossRef
  • TRATAMENTOS ALTERNATIVOS PARA TOXOPLASMOSE OCULAR: uma revisão integrativa
    Patricia Emanuella Ramos Marzola, Rodrigo Vasconcelos Marzola
    Arquivos Catarinenses de Medicina.2021; 49(4): 98.     CrossRef
  • Effectiveness of Treatments for Ocular Toxoplasmosis
    Julio Casoy, Heloisa Nascimento, Luci Meire P. Silva, Yuslay Fernández-Zamora, Cristina Muccioli, João Rafael de Oliveira Dias, Mário Junqueira Nóbrega, Heloísa Amaral Junqueira Nóbrega, Jacqueline Zummo, Rubens Belfort
    Ocular Immunology and Inflammation.2020; 28(2): 249.     CrossRef
  • Chorio-retinal toxoplasmosis: treatment outcomes, lesion evolution and long-term follow-up in a single tertiary center
    Vicktoria Vishnevskia-Dai, Asaf Achiron, Ortal Buhbut, Ofri Vorobichik Berar, Anne Ampaire Musika, Sivan M. Elyashiv, Idan Hecht
    International Ophthalmology.2020; 40(4): 811.     CrossRef
  • Toxoplasma gondii Parasitophorous Vacuole Membrane-Associated Dense Granule Proteins Regulate Maturation of the Cyst Wall
    Rebekah B. Guevara, Barbara A. Fox, David J. Bzik, Silvia N. J. Moreno
    mSphere.2020;[Epub]     CrossRef
  • Optical coherence tomography angiography findings in macular toxoplasma retinochoroiditis: A case report
    Alejandro L. Perez, Rosa A. Lozada, Andres Emanuelli, Armando L. Oliver
    American Journal of Ophthalmology Case Reports.2020; 19: 100764.     CrossRef
  • New findings useful for clinical practice using swept-source optical coherence tomography angiography in the follow-up of active ocular toxoplasmosis
    João Rafael de Oliveira Dias, Camila Campelo, Eduardo Amorim Novais, Gabriel Costa de Andrade, Paula Marinho, Yusláy Fernández Zamora, Luciana Finamor Peixoto, Maurício Maia, Heloísa Nascimento, Rubens Belfort
    International Journal of Retina and Vitreous.2020;[Epub]     CrossRef
  • Optical Coherence Tomography Angiography Analysis of Retinal and Choroidal Vascular Networks during Acute, Relapsing, and Quiescent Stages of Macular Toxoplasma Retinochoroiditis
    Georges Azar, Catherine Favard, Sawsen Salah, Antoine Brézin, Vivien Vasseur, Martine Mauget-Faÿsse, Luca Di Antonio
    BioMed Research International.2020;[Epub]     CrossRef
  • Dual-Stage Picolinic Acid-Derived Inhibitors of Toxoplasma gondii
    Muhammad M. Khalifa, Bruno Martorelli Di Genova, Sarah G. McAlpine, Gina M. Gallego-Lopez, David M. Stevenson, Soren D. Rozema, Neil P. Monaghan, James C. Morris, Laura J. Knoll, Jennifer E. Golden
    ACS Medicinal Chemistry Letters.2020; 11(12): 2382.     CrossRef
  • TOXOPLASMA NEURO-RETINITIS IN AN IMMUNO-COMPETENT ADULT- A CASE REPORT AND REVIEW OF LITERATURE
    Tharini Senthamizh, Subashini Kaliaperumal
    INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH.2020; : 1.     CrossRef
  • Bilateral Ocular Toxoplasmosis that Caused Optic Atrophy Secondary to Papillitis
    Donghun Lee, Minwoo Kim, Jong Won Moon
    Journal of the Korean Ophthalmological Society.2020; 61(9): 1109.     CrossRef
  • A white circular-spot pattern of iridian atrophy associated with Varicella-zoster virus and Toxoplasma gondii coinfection: a case report
    Juliana Muñoz-Ortiz, Olga Lorena Rubio-Romero, Maria Cecilia Cedeño, Karla Arteaga-Rivera, Alejandra de-la-Torre
    BMC Ophthalmology.2020;[Epub]     CrossRef
  • Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells
    Pu Reum Sun, Fei Fei Gao, Hei Gwon Choi, Wei Zhou, Jae-Min Yuk, Jaeyul Kwon, Young-Ha Lee, Guang-Ho Cha
    The Korean Journal of Parasitology.2019; 57(2): 83.     CrossRef
  • Management of Advanced HIV Disease
    Nathan A. Summers, Wendy S. Armstrong
    Infectious Disease Clinics of North America.2019; 33(3): 743.     CrossRef
  • Profil clinique et épidémiologique de la toxoplasmose oculaire à Kinshasa
    N. Nsiangani Lusambo, D. Kaimbo Wa Kaimbo
    Journal Français d'Ophtalmologie.2019; 42(8): 900.     CrossRef
  • Electrochemiluminescence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay), Tehran, Iran
    Ehsan Shariat Bahadory, Ali Dalir Ghaffari, Somayyeh Namrood, Seyyedeh Somayyeh Mosavipour, Javid Sadraie
    International Journal of Epidemiologic Research.2019; 6(2): 49.     CrossRef
  • Status of common parasitic diseases in Korea in 2019
    Sun Huh
    Journal of the Korean Medical Association.2019; 62(8): 437.     CrossRef
  • Fulminant Toxoplasmic Chorioretinitis Following Intravitreal Dexamethasone Implantation
    Areum Jeong, Dong-Geun Park, Min Sagong
    Journal of the Korean Ophthalmological Society.2019; 60(9): 896.     CrossRef
  • The Eye as a Window to Systemic Infectious Diseases: Old Enemies, New Imaging
    Vittorio Pirani, Paolo Pelliccioni, Serena De Turris, Alessandro Rosati, Alessandro Franceschi, Claudia Cesari, Michele Nicolai, Cesare Mariotti
    Journal of Clinical Medicine.2019; 8(9): 1392.     CrossRef
  • Association of TREM-1, IL-1β, IL-33/ST2, and TLR Expressions With the Pathogenesis of Ocular Toxoplasmosis in Mouse Models on Different Genetic Backgrounds
    Yanxia Zhang, Jian He, Huanqin Zheng, Shiguang Huang, Fangli Lu
    Frontiers in Microbiology.2019;[Epub]     CrossRef
  • Toxoplasma gondii Intravacuolar-Network-Associated Dense Granule Proteins Regulate Maturation of the Cyst Matrix and Cyst Wall
    Rebekah B. Guevara, Barbara A. Fox, Alejandra Falla, David J. Bzik, Silvia N. J. Moreno
    mSphere.2019;[Epub]     CrossRef
  • Ocular toxoplasmosis with surprisingly good retinal function
    Tove Lise Morisbakk, Per O Lundmark
    Scandinavian Journal of Optometry and Visual Science.2019; 12(1): 1.     CrossRef
  • Water related ocular diseases
    Syed Shoeb Ahmad
    Saudi Journal of Ophthalmology.2018; 32(3): 227.     CrossRef
  • Assessment of ocular toxoplasmosis patients reported at a tertiary center in the northeast of Iran
    Seyedeh Maryam Hosseini, Elham Moghaddas, Karim Sharifi, Malihe Dadgar Moghaddam, Seyed Aliakbar Shamsian
    International Ophthalmology.2018; 38(6): 2527.     CrossRef
  • Ocular Toxoplasmosis Mimicking Choroidal Metastasis From Colorectal Adenocarcinoma
    Samuel C. Fowler, Daniel R. Capiz-Correa, Hans E. Grossniklaus, Rebecca D. Shadowen, David A. Reichstein
    Journal of VitreoRetinal Diseases.2018; 2(6): 384.     CrossRef
  • A Perspective on Thiazolidinone Scaffold Development as a New Therapeutic Strategy for Toxoplasmosis
    Cristian Rocha-Roa, Diego Molina, Néstor Cardona
    Frontiers in Cellular and Infection Microbiology.2018;[Epub]     CrossRef
  • Diagnostic and Therapeutic Challenges
    Quraish Ghadiali, Orly Gal-Or, Jonathan Naysan, James Klancnik, K. Bailey Freund, SriniVas R. Sadda
    Retina.2018; 38(12): 2429.     CrossRef
  • Swept-source optical coherence tomography and optical coherence tomography angiography in acquired toxoplasmic chorioretinitis: a case report
    Diego Vezzola, Davide Allegrini, Alfredo Borgia, Paolo Fogagnolo, Luca Mario Rossetti, Mario R. Romano, Stefano De Cillà
    Journal of Medical Case Reports.2018;[Epub]     CrossRef
  • Analysis Concentration of <i>Toxoplasma gondii</i> on Anti-Toxoplasma IgG-IgM Antibody Levels, and the Outcomes of Pregnancy in Mice Balb/c
    Tigor Peniel Simanjuntak, Mochammad Hatta, Robert H. Sirait, Marni Br Karo, Lenny Irmawaty Sirait, Tetty Rina Aritonang, Syahrul Rauf, Ressy Dwiyanti
    Open Journal of Obstetrics and Gynecology.2017; 07(03): 281.     CrossRef
  • Recent Developments in the Diagnosis and Treatment of Ocular Toxoplasmosis
    Cem Ozgonul, Cagri Giray Besirli
    Ophthalmic Research.2017; 57(1): 1.     CrossRef
  • Infectious uveitis: An enigma
    Parthopratim Dutta Majumder, Avirupa Ghosh, Jyotirmay Biswas
    Middle East African Journal of Ophthalmology.2017; 24(1): 2.     CrossRef
  • Effect of Nigella sativa oil on experimental toxoplasmosis
    Rasha F. Mady, Wessam El-Hadidy, Samar Elachy
    Parasitology Research.2016; 115(1): 379.     CrossRef
  • Host and Toxoplasma gondii genetic and non-genetic factors influencing the development of ocular toxoplasmosis: A systematic review
    Carolina Fernández, Jesús Jaimes, María Camila Ortiz, Juan David Ramírez
    Infection, Genetics and Evolution.2016; 44: 199.     CrossRef
  • Ocular Inflammation Associated with Systemic Infection
    Byung Gil Moon, Joo Yong Lee
    Hanyang Medical Reviews.2016; 36(3): 192.     CrossRef
  • DNA Microarray Detection of 18 Important Human Blood Protozoan Species
    Mu-Xin Chen, Lin Ai, Jun-Hu Chen, Xin-Yu Feng, Shao-Hong Chen, Yu-Chun Cai, Yan Lu, Xiao-Nong Zhou, Jia-Xu Chen, Wei Hu, Enock Matovu
    PLOS Neglected Tropical Diseases.2016; 10(12): e0005160.     CrossRef
  • Macular infarction in a patient with Toxoplasma retinochoroditis
    Vinod Kumar, Brijesh Takkar, Parijat Chandra, Atul Kumar
    BMJ Case Reports.2016; 2016: bcr2015212596.     CrossRef
  • Müller cell activation and photoreceptor depletion in a mice model of congenital ocular toxoplasmosis
    Ibtissem Lahmar, Alexander W. Pfaff, Luc Marcellin, Arnaud Sauer, Adnane Moussa, Hamouda Babba, Ermanno Candolfi
    Experimental Parasitology.2014; 144: 22.     CrossRef
  • Neuropsychiatric manifestations of latent toxoplasmosis on mothers and their offspring
    Amir Abdoli, Abdolhossein Dalimi, Mohsen Arbabi, Fatemeh Ghaffarifar
    The Journal of Maternal-Fetal & Neonatal Medicine.2014; 27(13): 1368.     CrossRef
  • Excretory–secretory antigens: A suitable candidate for immunization against ocular toxoplasmosis in a murine model
    Kiumars Norouzpour Deilami, Ahmad Daryani, Ehsan Ahmadpour, Mehdi Sharif, Yousef Dadimoghaddam, Shahabeddin Sarvi, Ahad Alizadeh
    Comparative Immunology, Microbiology and Infectious Diseases.2014; 37(5-6): 369.     CrossRef
  • 20,861 View
  • 340 Download
  • Crossref

Brief Communications

Prevalence and Genetic Characterization of Toxoplasma gondii in House Sparrows (Passer domesticus) in Lanzhou, China
Wei Cong, Si-Yang Huang, Dong-Hui Zhou, Xiao-Xuan Zhang, Nian-Zhang Zhang, Quan Zhao, Xing-Quan Zhu
Korean J Parasitol 2013;51(3):363-367.
Published online June 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.3.363

The prevalence of Toxoplasma gondii infection in birds has epidemiological significance because birds are indeed considered as a good indicator of environmental contamination by T. gondii oocysts. In this study, the prevalence of T. gondii in 313 house sparrows in Lanzhou, northwestern China was assayed by the modified agglutination test (MAT). Antibodies to T. gondii were positive in 39 (12.46%) of 313 samples (MAT titer ≥ 1:5). Tissues of heart, brain, and lung from the 39 seropositive house sparrows were tested for T. gondii DNA, 11 of which were found to be positive for the T. gondii B1 gene by PCR amplification. These positive DNA samples were typed at 9 genetic markers, including 8 nuclear loci, i.e., SAG1, 5'- and 3'-SAG2, alternative SAG2, SAG3, GRA6, L358, PK1, c22-8 and an apicoplast locus Apico. Of them, 4 isolates were genotyped with complete data for all loci, and 2 genotypes (Type II variants; ToxoDB #3 and a new genotype) were identified. These results showed that there is a potential risk for human infection with T. gondii in this region. To our knowledge, this is the first report of T. gondii seroprevalence in house sparrows in China.

Citations

Citations to this article as recorded by  Crossref logo
  • Global prevalence and risk factors associated with Toxoplasma gondii infection in wild birds: A systematic review and meta-analysis
    Chao Chen, Si-Yuan Qin, Xing Yang, Xiao-Man Li, Yanan Cai, Cong-Cong Lei, Quan Zhao, Hany M. Elsheikha, Hongwei Cao
    Preventive Veterinary Medicine.2024; 226: 106187.     CrossRef
  • Molecular detection of Toxoplasma gondii (Chromista: Apicomplexa) in the blood of passerines (Aves: Passeriformes) in south-eastern Armenia
    Sargis A. Aghayan, Manan Asikyan, Marko Raković, Daliborka Stanković, Igor V. Fadeev, Hasmik Gevorgyan, Oleg Shcherbakov, Marine Arakelyan, Karen Aghababyan, Abdol Sattar Pagheh, Mehdi Sharif, Ahmad Daryani
    Zoologia (Curitiba).2024;[Epub]     CrossRef
  • Two viable Toxoplasma gondii isolates from red-necked wallaby (Macropus rufogriseus) and red kangaroo (M. rufus)
    Liulu Yang, Shilin Xin, Niuping Zhu, Junbao Li, Chunlei Su, Yurong Yang
    Parasitology International.2023; 92: 102687.     CrossRef
  • Epidemiology and isolation of viable Toxoplasma gondii strain from macropods
    Liulu Yang, Hongjie Ren, Niuping Zhu, Gaohui Mao, Junbao Li, Chunlei Su, Yibao Jiang, Yurong Yang
    Heliyon.2023; 9(3): e13960.     CrossRef
  • Genetic diversity of Toxoplasma gondii isolates from birds in the world: A systematic review
    Tahereh Mikaeili Galeh, Shahabeddin Sarvi, Alireza Khalilian, Seyed Abdollah Hosseini, Ahmad Daryani
    Experimental Parasitology.2023; 248: 108480.     CrossRef
  • Metagenomic insights into the composition and function of the gut microbiota of mice infected with Toxoplasma gondii
    Jin-Xin Meng, Xin-Yu Wei, Huanping Guo, Yu Chen, Wei Wang, Hong-Li Geng, Xing Yang, Jiang Jiang, Xiao-Xuan Zhang
    Frontiers in Immunology.2023;[Epub]     CrossRef
  • Low Prevalence of Toxoplasma gondii in Sheep and Isolation of a Viable Strain from Edible Mutton from Central China
    Yibao Jiang, Shilin Xin, Yiheng Ma, Heng Zhang, Xu Yang, Yurong Yang
    Pathogens.2023; 12(6): 827.     CrossRef
  • Abundance of Non-Native Birds in the City: Spatial Variation and Relationship with Socioeconomics in a South American City
    Macarena Silva-Ortega, Catalina B. Muñoz-Pacheco, Nélida R. Villaseñor
    Animals.2023; 13(11): 1737.     CrossRef
  • Investigation of Toxoplasma gondii in wastewater and surface water in the Qinghai-Tibet Plateau, China using real-time PCR and multilocus genotyping
    Anna Lass, Ioannis Kontogeorgos, Liqing Ma, Xueyong Zhang, Xiuping Li, Panagiotis Karanis
    Scientific Reports.2022;[Epub]     CrossRef
  • Epidemiologic significance of Toxoplasma gondii infections in turkeys, ducks, ratites and other wild birds: 2009–2020
    J. P. Dubey, F. H. A. Murata, C. K. Cerqueira-Cézar, O. C. H. Kwok, C. Su
    Parasitology.2021; 148(1): 1.     CrossRef
  • Molecular Prevalence and Genetic Characterization of Toxoplasma gondii in Wild Birds in Hunan Province, China
    Meng-Ting Liu, Wei-Xing Jiang, Bin-Ze Gui, Yuan-Chun Jin, Jia-Ning Yi, Fen Li, Wen-Bin Zheng, Guo-Hua Liu
    Vector-Borne and Zoonotic Diseases.2019; 19(5): 378.     CrossRef
  • Seroprevalence and risk assessment of Toxoplasma gondii in Java sparrows (Lonchura oryzivora) in China
    Si-Yang Huang, Yi-Min Fan, Kai Chen, Qiu-Xia Yao, Bin Yang
    BMC Veterinary Research.2019;[Epub]     CrossRef
  • Prevalence, risk factors and genetic characterization of Toxoplasma gondii in sick pigs and stray cats in Jiangsu Province, eastern China
    Zhao-feng Hou, Shi-jie Su, Dan-dan Liu, Le-le Wang, Chuan-li Jia, Zhen-xing Zhao, Yi-fei Ma, Qiao-qiao Li, Jin-jun Xu, Jian-ping Tao
    Infection, Genetics and Evolution.2018; 60: 17.     CrossRef
  • First genetic characterization of Toxoplasma gondii infection in common quails (Coturnix coturnix) intended for human consumption in China
    Wei Cong, Hong-Liang Ju, Xiao-Xuan Zhang, Qing-Feng Meng, Jian-Gang Ma, Ai-Dong Qian, Xing-Quan Zhu
    Infection, Genetics and Evolution.2017; 49: 14.     CrossRef
  • Prevalence and genetic characterization of Toxoplasma gondii in badgers ( Melogale moschata ) in southern China by PCR-RFLP
    Kai Chen, Si-Yang Huang, Jin-Lei Wang, Rong-Liang Hu, Qiu-Xia Yao, Shou-Feng Zhang, Xing-Quan Zhu, Quan Liu
    Infection, Genetics and Evolution.2017; 52: 30.     CrossRef
  • Geographical distribution of Toxoplasma gondii genotypes in Asia: A link with neighboring continents
    P. Chaichan, A. Mercier, L. Galal, A. Mahittikorn, F. Ariey, S. Morand, F. Boumédiène, R. Udonsom, A. Hamidovic, J.B. Murat, Y. Sukthana, M.L. Dardé
    Infection, Genetics and Evolution.2017; 53: 227.     CrossRef
  • Sixty Years (1957–2017) of Research on Toxoplasmosis in China—An Overview
    Ming Pan, Congcong Lyu, Junlong Zhao, Bang Shen
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • Molecular detection of Toxoplasma gondii in house sparrow (Passer domesticus) by LAMP and PCR methods in Tehran, Iran
    Amir Abdoli, Abdolhossein Dalimi, Haleh Soltanghoraee, Fatemeh Ghaffarifar
    Journal of Parasitic Diseases.2016; 40(4): 1317.     CrossRef
  • Seroprevalence and genotypes of Toxoplasma gondii isolated from pigs intended for human consumption in Liaoning province, northeastern China
    Dawei Wang, Yan Liu, Tiantian Jiang, Guoxin Zhang, Gaoming Yuan, Jianbin He, Chunlei Su, Na Yang
    Parasites & Vectors.2016;[Epub]     CrossRef
  • Multi-scale occupancy approach to estimate Toxoplasma gondii prevalence and detection probability in tissues: an application and guide for field sampling
    Stacey A. Elmore, Kathryn P. Huyvaert, Larissa L. Bailey, Asma Iqbal, Chunlei Su, Brent R. Dixon, Ray T. Alisauskas, Alvin A. Gajadhar, Emily J. Jenkins
    International Journal for Parasitology.2016; 46(9): 563.     CrossRef
  • Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP
    Wen-Bin Zheng, Xiao-Xuan Zhang, Jian-Gang Ma, Fa-Cai Li, Quan Zhao, Si-Yang Huang, Xing-Quan Zhu, Gordon Langsley
    PLOS ONE.2016; 11(11): e0165308.     CrossRef
  • Toxoplasma gondii infection in cancer patients: Prevalence, risk factors, genotypes and association with clinical diagnosis
    Wei Cong, Guo-Hua Liu, Qing-Feng Meng, Wei Dong, Si-Yuan Qin, Fu-Kai Zhang, Xiang-Yan Zhang, Xiang-Yang Wang, Ai-Dong Qian, Xing-Quan Zhu
    Cancer Letters.2015; 359(2): 307.     CrossRef
  • Prevalence of <i>Toxoplasma gondii</i> in Dogs in Zhanjiang, Southern China
    Hai-Hai Jiang, Ming-Wei Li, Min-Jun Xu, Wei Cong, Xing-Quan Zhu
    The Korean Journal of Parasitology.2015; 53(4): 493.     CrossRef
  • Prevalence of Antibody toToxoplasma gondiiin Black-headed Gulls (Chroicocephalus ridibundus), Dianchi Lake, China
    Qiang Miao, Jiang-qiang Han, Xun Xiang, Fei-Zhou Yuan, Yong-zhang Liu, Gang Duan, Xing-quan Zhu, Feng-cai Zou
    Journal of Wildlife Diseases.2014; 50(3): 717.     CrossRef
  • Seroprevalence and genetic characterization of Toxoplasma gondii in three species of pet birds in China
    Wei Cong, Qing-Feng Meng, Hui-Qun Song, Dong-Hui Zhou, Si-Yang Huang, Ai-Dong Qian, Chunlei Su, Xing-Quan Zhu
    Parasites & Vectors.2014;[Epub]     CrossRef
  • First Report of Genotyping ofToxoplasma gondiiin Free-LivingMicrotus fortisin Northeastern China
    Xiao-Xuan Zhang, Si-Yang Huang, Ying-Guang Zhang, Yuan Zhang, Xing-Quan Zhu, Quan Liu
    Journal of Parasitology.2014; 100(5): 692.     CrossRef
  • Genetic characterization of Toxoplasma gondii from cats in Yunnan Province, Southwestern China
    Yi-Ming Tian, Si-Yang Huang, Qiang Miao, Hai-Hai Jiang, Jian-Fa Yang, Chunlei Su, Xing-Quan Zhu, Feng-Cai Zou
    Parasites & Vectors.2014;[Epub]     CrossRef
  • Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP
    Hai-Hai Jiang, Si-Yang Huang, Dong-Hui Zhou, Xiao-Xuan Zhang, Chunlei Su, Shun-Zhou Deng, Xing-Quan Zhu
    Parasites & Vectors.2013;[Epub]     CrossRef
  • 11,268 View
  • 93 Download
  • Crossref
Prevalence of Toxoplasma gondii Infection in Household Cats in Korea and Risk Factors
Sung-Hee Hong, Young-Il Jeong, Jae-Young Kim, Shin-Hyeong Cho, Won-Ja Lee, Sang-Eun Lee
Korean J Parasitol 2013;51(3):357-361.
Published online June 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.3.357

Several epidemiological surveys have reported the prevalence of Toxoplasma gondii infection in stray cats in Korea, but little information is available on T. gondii infection in household cats. The aim of the present study was to assess the prevalence and risk factors of T. gondii infection among household cats reared in Seoul, Korea. A total of 474 blood samples were collected from clinically healthy household cats. All samples were tested using ELISA and PCR. The risk factor analysis was based on a questionnaire filled out by the owners. The overall positive rate for ELISA and PCR assays was 2.2% (10/437) and 2.1% (10/474), respectively. With regard to the origin of cats, the positive rates among cats adopted from the animal shelter and veterinary clinic for stray cats were significantly different (P<0.05). Our study demonstrated that the positive rate of T. gondii infection in household cats was low and that this low prevalence was assumed to be associated with keeping the cats indoors and restriction of eating raw food and uncooked meat. Therefore, we suggest that the owners check the origin of the cats prior to adoption to prevent infection of other animals, including humans.

Citations

Citations to this article as recorded by  Crossref logo
  • Prevalence of selected infectious agents in Swedish cats with fever and/or anemia compared to cats without fever and/or anemia and to stable/stray cats
    Gunilla Ölmedal, Linda Toresson, Mary Nehring, Jennifer Hawley, Sue Vande Woude, Michael Lappin
    Acta Veterinaria Scandinavica.2025;[Epub]     CrossRef
  • Seroprevalence of and Risk Factors for Toxoplasma gondii Infection in Cats from Greece
    Kassiopi Christina G. Kokkinaki, Manolis N. Saridomichelakis, Mathios E. Mylonakis, Leonidas Leontides, Panagiotis G. Xenoulis
    Animals.2023; 13(7): 1173.     CrossRef
  • Establishment and application of an iELISA detection method for measuring apical membrane antigen 1 (AMA1) antibodies of Toxoplasma gondii in cats
    Yafan Gao, Yu Shen, Jiyuan Fan, Haojie Ding, Bin Zheng, Haijie Yu, Siyang Huang, Qingming Kong, Hangjun Lv, Xunhui Zhuo, Shaohong Lu
    BMC Veterinary Research.2023;[Epub]     CrossRef
  • Prevalence of Toxoplasma gondii Measured by Western Blot, ELISA and DNA Analysis, by PCR, in Cats of Western Mexico
    María de la Luz Galván-Ramírez, Claudia Charles-Niño, César Pedroza-Roldán, Carolina Salazar-Reveles, Karen Lissete Ocampo-Figueroa, Laura Roció Rodríguez-Pérez, Varinia Margarita Paez-Magallán
    Pathogens.2022; 11(1): 109.     CrossRef
  • Prevalence of toxoplasmosis in semi-domesticated and pet cats within and around Bangkok, Thailand
    Tawin Inpankaew, Panpicha Sattasathuchana, Chanya Kengradomkij, Naris Thengchaisri
    BMC Veterinary Research.2021;[Epub]     CrossRef
  • All about toxoplasmosis in cats: the last decade
    J.P. Dubey, C.K. Cerqueira-Cézar, F.H.A. Murata, O.C.H. Kwok, Y.R. Yang, C. Su
    Veterinary Parasitology.2020; 283: 109145.     CrossRef
  • Seroprevalence and Risk Factors of Toxoplasma gondii Infection among Cat Sitters in Korea
    Bong-Kwang Jung, Hyemi Song, Sang-Eun Lee, Min-Jae Kim, Jaeeun Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2017; 55(2): 203.     CrossRef
  • Serological and Molecular Detection of Toxoplasma gondii and Babesia microti in the Blood of Rescued Wild Animals in Gangwon-do (Province), Korea
    Sung-Hee Hong, Hee-Jong Kim, Young-Il Jeong, Shin-Hyeong Cho, Won-Ja Lee, Jong-Tak Kim, Sang-Eun Lee
    The Korean Journal of Parasitology.2017; 55(2): 207.     CrossRef
  • Molecular investigation on the occurrence of Toxoplasma gondii oocysts in cat feces using TOX-element and ITS-1 region targets
    W. Chemoh, N. Sawangjaroen, V. Nissapatorn, N. Sermwittayawong
    The Veterinary Journal.2016; 215: 118.     CrossRef
  • <i>Toxoplasma gondii</i> B1 Gene Detection in Feces of Stray Cats around Seoul, Korea and Genotype Analysis of Two Laboratory-Passaged Isolates
    Bong-Kwang Jung, Sang-Eun Lee, Hyemi Lim, Jaeeun Cho, Deok-Gyu Kim, Hyemi Song, Min-Jae Kim, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2015; 53(3): 259.     CrossRef
  • A high seroprevalence ofToxoplasma gondiiantibodies in a population of feral cats in the Western Cape province of South Africa
    Kenneth Hammond-Aryee, Monika Esser, Lesley van Helden, Paul van Helden
    Southern African Journal of Infectious Diseases.2015; 30(4): 141.     CrossRef
  • 9,965 View
  • 97 Download
  • Crossref

Original Articles

Proliferation of Toxoplasma gondii Suppresses Host Cell Autophagy
Youn-Jin Lee, Hyun-Ouk Song, Young-Ha Lee, Jae-Sook Ryu, Myoung-Hee Ahn
Korean J Parasitol 2013;51(3):279-287.
Published online June 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.3.279

Autophagy is a process of cytoplasmic degradation of endogenous proteins and organelles. Although its primary role is protective, it can also contribute to cell death. Recently, autophagy was found to play a role in the activation of host defense against intracellular pathogens. The aims of our study was to investigate whether host cell autophagy influences Toxoplasma gondii proliferation and whether autophagy inhibitors modulate cell survival. HeLa cells were infected with T. gondii with and without rapamycin treatment to induce autophagy. Lactate dehydrogenase assays showed that cell death was extensive at 36-48 hr after infection in cells treated with T. gondii with or without rapamycin. The autophagic markers, LC3 II and Beclin 1, were strongly expressed at 18-24 hr after exposure as shown by Western blotting and RT-PCR. However, the subsequent T. gondii proliferation suppressed autophagy at 36 hr post-infection. Pre-treatment with the autophagy inhibitor, 3-methyladenine (3-MA), down-regulated LC3 II and Beclin 1. The latter was also down-regulated by calpeptin, a calpain inhibitor. Monodansyl cadaverine (MDC) staining detected numerous autophagic vacuoles (AVs) at 18 hr post-infection. Ultrastructural observations showed T. gondii proliferation in parasitophorous vacuoles (PVs) coinciding with a decline in the numbers of AVs by 18 hr. FACS analysis failed to confirm the presence of cell apoptosis after exposure to T. gondii and rapamycin. We concluded that T. gondii proliferation may inhibit host cell autophagy and has an impact on cell survival.

Citations

Citations to this article as recorded by  Crossref logo
  • Modulation of autophagy as a therapeutic strategy for Toxoplasma gondii infection
    Ao Cheng, Huanan Zhang, Baike Chen, Shengyao Zheng, Hongyi Wang, Yijia Shi, Siyao You, Ming Li, Liping Jiang
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • Myrislignan Induces Redox Imbalance and Activates Autophagy in Toxoplasma gondii
    Jili Zhang, Jia Chen, Kun Lv, Bing Li, Biqing Yan, Lei Gai, Chaolu Shi, Xinnian Wang, Hongfei Si, Jiyu Zhang
    Frontiers in Cellular and Infection Microbiology.2021;[Epub]     CrossRef
  • 4-Hydroxybenzaldehyde Restricts the Intracellular Growth of Toxoplasma gondii by Inducing SIRT1-Mediated Autophagy in Macrophages
    Jina lee, Jae-Won Choi, Hye Young Han, Woo Sik Kim, Ha-Yeon Song, Eui-Baek Byun, Eui-Hong Byun, Young-Ha Lee, Jae-Min Yuk
    The Korean Journal of Parasitology.2020; 58(1): 7.     CrossRef
  • Foot-and-mouth disease virus induces PERK mediated autophagy to suppress antiviral interferon response
    H. B. Ranjitha, Veena Ammanathan, Neha Guleria, Madhusudan Hosamani, B. P. Sreenivasa, V. V. Dhanesh, Rashmi Santhoshkumar, B. K. Chandrasekhar Sagar, B. P. Mishra, R. K. Singh, Aniket Sanyal, Ravi Manjithaya, Suresh H. Basagoudanavar
    Journal of Cell Science.2020;[Epub]     CrossRef
  • The Nrf2 pathway is required for intracellular replication ofToxoplasma gondiiin activated macrophages
    Yu Pang, Zhaoxia Zhang, Yuntong Chen, Shinuo Cao, Xiuqin Yang, Honglin Jia
    Parasite Immunology.2019;[Epub]     CrossRef
  • Host-Toxoplasma gondii Coadaptation Leads to Fine Tuning of the Immune Response
    Thaís Rigueti Brasil, Celio Geraldo Freire-de-Lima, Alexandre Morrot, Andrea Cristina Vetö Arnholdt
    Frontiers in Immunology.2017;[Epub]     CrossRef
  • Anti-Toxoplasmosis Effect of the Herbal Extracts Plantago asiatica L.
    Sunhwa Hong, Okjin Kim
    Korean Journal of Plant Resources.2016; 29(1): 26.     CrossRef
  • Autophagy activated by Toxoplasma gondii infection in turn facilitates Toxoplasma gondii proliferation
    Dongmei Gao, Jing Zhang, Jun Zhao, He Wen, Jiwen Pan, Shouzhu Zhang, Yong Fang, Xiuyi Li, Yu Cai, Xuelong Wang, Shiping Wang
    Parasitology Research.2014; 113(6): 2053.     CrossRef
  • Anti-Toxoplasmosis Effect of the Halophyte Suaeda maritime
    Sunhwa Hong, Hyun-A Lee, Yun-Seong Lee, Dong-Woo Kim, Jae-Hyeok Jeong, Tae-Wan Kim, Okjin Kim
    Korean Journal of Plant Resources.2014; 27(5): 415.     CrossRef
  • 12,000 View
  • 123 Download
  • Crossref
Kinetics of IL-23 and IL-12 Secretion in Response to Toxoplasma gondii Antigens from THP-1 Monocytic Cells
Juan-Hua Quan, Wei Zhou, Guang-Ho Cha, In-Wook Choi, Dae-Whan Shin, Young-Ha Lee
Korean J Parasitol 2013;51(1):85-92.
Published online February 18, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.1.85

IL-23 and IL-12 are structurally similar and critical for the generation of efficient cellular immune responses. Toxoplasma gondii induces a strong cell-mediated immune response. However, little is known about IL-23 secretion profiles in T. gondii-infected immune cells in connection with IL-12. We compared the patterns of IL-23 and IL-12 production by THP-1 human monocytic cells in response to stimulation with live or heat-killed T. gondii tachyzoites, or with equivalent quantities of either T. gondii excretory/secretory proteins (ESP) or soluble tachyzoite antigen (STAg). IL-23 and IL-12 were significantly increased from 6 hr after stimulation with T. gondii antigens, and their secretions were increased with parasite dose-dependent manner. IL-23 concentrations were significantly higher than those of IL-12 at the same multiplicity of infection. IL-23 secretion induced by live parasites was significantly higher than that by heat-killed parasites, ESP, or STAg, whereas IL-12 secretion by live parasite was similar to those of ESP or STAg. However, the lowest levels of both cytokines were at stimulation with heat-killed parasites. These data indicate that IL-23 secretion patterns by stimulation with various kinds of T. gondii antigens at THP-1 monocytic cells are similar to those of IL-12, even though the levels of IL-23 induction were significantly higher than those of IL-12. The detailed kinetics induced by each T. gondii antigen were different from each other.

Citations

Citations to this article as recorded by  Crossref logo
  • Differential detection of chicken heterodimeric cytokines, interleukin 12 and 23 using their subunit-specific mouse monoclonal antibodies
    Youngsub Lee, Woo H. Kim, Hyoyoun Nam, Hyun S. Lillehoj
    Poultry Science.2024; 103(8): 103872.     CrossRef
  • T. gondii excretory proteins promote the osteogenic differentiation of human bone mesenchymal stem cells via the BMP/Smad signaling pathway
    Mingzhu Deng, Feifei Gao, Tianfeng Liu, Weiqiang Zhan, Juanhua Quan, Ziquan Zhao, Xuyang Wu, Zhuolan Zhong, Hong Zheng, Jiaqi Chu
    Journal of Orthopaedic Surgery and Research.2024;[Epub]     CrossRef
  • IL-12 and IL-23 Production in Toxoplasma gondii- or LPS Treated Jurkat T Cells via PI3K and MAPK Signaling Pathways
    Hassan Ahmed Hassan Ahmed Ismail, Byung-Hun Kang, Jae-Su Kim, Jae-Hyung Lee, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee
    The Korean Journal of Parasitology.2017; 55(6): 613.     CrossRef
  • Cytokine response of human THP-1 macrophages to Trichomonas tenax
    Emily J. Govro, Melissa K. Stuart
    Experimental Parasitology.2016; 169: 77.     CrossRef
  • Far beyond Phagocytosis: Phagocyte-Derived Extracellular Traps Act Efficiently against Protozoan ParasitesIn VitroandIn Vivo
    Liliana M. R. Silva, Tamara Muñoz-Caro, Rafael A. Burgos, Maria A. Hidalgo, Anja Taubert, Carlos Hermosilla
    Mediators of Inflammation.2016; 2016: 1.     CrossRef
  • Eimeria ninakohlyakimovae induces NADPH oxidase-dependent monocyte extracellular trap formation and upregulates IL-12 and TNF-α, IL-6 and CCL2 gene transcription
    D. Pérez, M.C. Muñoz, J.M. Molina, T. Muñoz-Caro, L.M.R. Silva, A. Taubert, C. Hermosilla, A. Ruiz
    Veterinary Parasitology.2016; 227: 143.     CrossRef
  • Secretion of Rhoptry and Dense Granule Effector Proteins by Nonreplicating Toxoplasma gondii Uracil Auxotrophs Controls the Development of Antitumor Immunity
    Barbara A. Fox, Kiah L. Sanders, Leah M. Rommereim, Rebekah B. Guevara, David J. Bzik, Imtiaz A Khan
    PLOS Genetics.2016; 12(7): e1006189.     CrossRef
  • Levels of Transforming Growth Factor-Beta After Immunization of Mice With Toxoplasma gondii prepared Excretory/Secretory Proteins
    Seyed Hossein Abdollahi, Fateme Ayoobi, Hossein Khorramdelazad, Behzad Nasiri Ahmadabadi, Mohammadtaghi Rezayati, Mohammad Kazemi Arababadi, Mohammad Zare-Bidaki
    Jundishapur Journal of Microbiology.2015;[Epub]     CrossRef
  • Intracellular Networks of the PI3K/AKT and MAPK Pathways for Regulating Toxoplasma gondii-Induced IL-23 and IL-12 Production in Human THP-1 Cells
    Juan-Hua Quan, Jia-Qi Chu, Jaeyul Kwon, In-Wook Choi, Hassan Ahmed Hassan Ahmed Ismail, Wei Zhou, Guang-Ho Cha, Yu Zhou, Jae-Min Yuk, Eun-Kyeong Jo, Young-Ha Lee, Salvatore V Pizzo
    PLOS ONE.2015; 10(11): e0141550.     CrossRef
  • Besnoitia besnoiti tachyzoites induce monocyte extracellular trap formation
    Tamara Muñoz-Caro, Liliana M. R. Silva, Christin Ritter, Anja Taubert, Carlos Hermosilla
    Parasitology Research.2014; 113(11): 4189.     CrossRef
  • 12,557 View
  • 112 Download
  • Crossref

Brief Communication

Evaluation of Anti-Toxoplasma IgG, IgM, and IgA in Mothers with Spontaneous Abortion in Zanjan, Northwest Iran
Abbas Amin, S. Mazloomzadeh, A. Haniloo, F. Mohammadian, Asghar Fazaeli
Korean J Parasitol 2012;50(4):371-374.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.371

Toxoplasma gondii is one of the major agents of infectious abortions and due to its worldwide distribution can threat healthy pregnant women who had no previous exposure to this parasite. The present study was designed to investigate the contribution of T. gondii to spontaneous abortions in Zanjan, Northwest of Iran, using ELISA method. Blood Samples were collected from 264 mothers referred to the provincial hospitals of Zanjan due to spontaneous abortion. The sera were isolated and subjected to evaluate the anti-Toxoplasma IgG, IgM and IgA antibodies. The results showed IgG positive (IgG+) in 99 cases (37.5%). A total of 68 women (25.8%) showed seroconversion with IgM or IgA or both IgM and IgA. They included: IgM+ in 21 (8.0%), IgA+ in 23 (8.7%) and both IgM+ and IgA+ in 24 (9.1%) subjects. In 23 cases, positive titers of IgM and IgG were accompanied. In general, the analysis of anti-Toxoplasma antibody patterns, showed that about 17% of the spontaneous abortions were associated with serological patterns of acute infection. According to these findings, a considerable proportion of spontaneous abortions can be attributed to T. gondii in the study area.

Citations

Citations to this article as recorded by  Crossref logo
  • Screening for the Detection of Toxoplasma gondii IgG, IgM and IgA in Females of Reproductive Age from Western Romania
    Alin Gabriel Mihu, Maria Alina Lupu, Alexandru Nesiu, Daniela Teodora Marti, Tudor Rares Olariu
    Life.2022; 12(11): 1771.     CrossRef
  • Toxoplasma gondii infection and spontaneous abortion: A systematic review and meta-analysis
    Narges Kalantari, Tahmineh Gorgani-Firouzjaee, Zahra Moulana, Mohammad Chehrazi, Salman Ghaffari
    Microbial Pathogenesis.2021; 158: 105070.     CrossRef
  • The global seroprevalence of anti-Toxoplasma gondii antibodies in women who had spontaneous abortion: A systematic review and meta-analysis
    Tooran Nayeri, Shahabeddin Sarvi, Mahmood Moosazadeh, Afsaneh Amouei, Zahra Hosseininejad, Ahmad Daryani, Maria Angeles Gómez-Morales
    PLOS Neglected Tropical Diseases.2020; 14(3): e0008103.     CrossRef
  • Seroprevalence and Risk Factors of Toxoplasma gondii Infection in Pregnant Women from Western Romania
    Tudor Rares Olariu, Sorin Ursoniu, Ionela Hotea, Victor Dumitrascu, Doru Anastasiu, Maria Alina Lupu
    Vector-Borne and Zoonotic Diseases.2020; 20(10): 763.     CrossRef
  • Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1α) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis
    Shuai Wang, Zhenchao Zhang, Yujian Wang, Javaid A. Gadahi, Lixin Xu, Ruofeng Yan, Xiaokai Song, Xiangrui Li
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • Serological study of toxoplasmosis in women with previous history of abortion at Hamedan’s medical centers during 2012–2013
    M. Abedi, H. Heidari, Z. Sadeghi Dehkordi, M. R. Youssefi
    Comparative Clinical Pathology.2015; 24(3): 589.     CrossRef
  • Sequence Diversity in <i>MIC6</i> Gene among <i>Toxoplasma gondii</i> Isolates from Different Hosts and Geographical Locations
    Zhong-Yuan Li, Hui-Qun Song, Jia Chen, Xing-Quan Zhu
    The Korean Journal of Parasitology.2015; 53(3): 341.     CrossRef
  • Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii 10kDa excretory–secretory antigen (TgESA10)
    Shuai Wang, Yujian Wang, Xiaoni Sun, Zhenchao Zhang, Tingqi Liu, Javaid Ali Gadahi, Lixin Xu, Ruofeng Yan, Xiaokai Song, Xiangrui Li
    Veterinary Parasitology.2015; 214(1-2): 40.     CrossRef
  • Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha
    Shuai Wang, YuJian Wang, XiaoNi Sun, ZhenChao Zhang, TingQi Liu, Javaid Ali Gadahi, Ibrahim Adam Hassan, LiXin Xu, RuoFeng Yan, XiaoKai Song, XiangRui Li
    BMC Infectious Diseases.2015;[Epub]     CrossRef
  • 11,784 View
  • 154 Download
  • Crossref

Original Article

Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
Hyemi Lim, Sang-Eun Lee, Bong-Kwang Jung, Min-Ki Kim, Mi Youn Lee, Ho-Woo Nam, Jong-Gyun Shin, Cheong-Ha Yun, Han-Ik Cho, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2012;50(4):287-293.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.287

Knowledge of the prevalence of human Toxoplasma gondii infection is required in the Republic of Korea. In this study, we surveyed the seroprevalence of T. gondii infection and analyzed the risk factors associated with seropositivity among residents in 2 administrative districts; Seoul and the island of Jeju-do, which have contrasting epidemiologic characteristics. Sera and blood collected from 2,150 residents (1,114 in Seoul and 1,036 in Jeju-do) were checked for IgG antibody titers using ELISA and for the T. gondii B1 gene using PCR. In addition, participants completed a questionnaire that solicited information on gender, age, occupation, eating habits, history of contact with animals, and travel abroad. The T. gondii B1 gene was not detected in all residents examined. However, ELISA showed 8.0% (89 of 1,114 sera) positive for IgG antibodies against T. gondii in Seoul and 11.3% (117 of 1,036 sera) in Jeju-do. In both districts, the positive rates were higher in males than in females, and those 40-79 years of age showed higher rates than other ages. In Seoul, residents older than 70 years of age showed the highest positive rate, 14.9%, whereas in Jeju-do the highest prevalence, 15.6%, was in those in their sixties. The higher seropositive rate in Jeju-do than in Seoul may be related to eating habits and occupations. The present results and a review of related literature are indicative of an increased seroprevalence of T. gondii in Korea in recent years.

Citations

Citations to this article as recorded by  Crossref logo
  • Molecular detection of Toxoplasma gondii in wild oysters and seawater on the southeast coast of South Korea
    Hyejoo Shin, Sooji Hong, Seungwan Ryoo, Jong-Yil Chai, Bong-Kwang Jung
    Food Microbiology.2026; 134: 104925.     CrossRef
  • Clinical characteristics of toxoplasmosis patients in Korea: A retrospective study using health insurance review and assessment service data and electronic medical records
    Do-Won Ham, Bong-Kwang Jung, Ji-Hun Shin, Yong Joon Kim, Kyoung Yul Seo, Seung Mi Lee, Jae Hyoung Im, Jeong-Ran Kwon, Ho-Sung Lee, Kyung-Won Hwang, Eun-Hee Shin
    Parasites, Hosts and Diseases.2024; 62(4): 424.     CrossRef
  • Exosomal miRNA-21 from Toxoplasma gondii-infected microglial cells induces the growth of U87 glioma cells by inhibiting tumor suppressor genes
    Bong-Kwang Jung, Hyemi Song, Hyejoo Shin, Jong-Yil Chai
    Scientific Reports.2022;[Epub]     CrossRef
  • Toxoplasmosis in pregnancy: a neglected bane but a serious threat in Nigeria
    Oyetunde T. Oyeyemi, Ifeoluwa T. Oyeyemi, Isaac A. Adesina, Adebisi M. Tiamiyu, Yinka D. Oluwafemi, Roseangela I. Nwuba, Rafaella F. Q. Grenfell
    Parasitology.2020; 147(2): 127.     CrossRef
  • Seroepidemiology and risk factors of toxoplasmosis among children age ranged from 1 to 14 years referred to medical diagnostic laboratories in Southeast Iran
    Vahid Raissi, Fatemeh Bayat, Ali Taghipour, Omid Raiesi, Asmaa Ibrahim, Muhammad Getso, Ziba Hoseiny, Gita Alizadeh, Mehdi Khoshsima Shahraki, Soudabeh Etemadi
    Clinical Epidemiology and Global Health.2020; 8(2): 595.     CrossRef
  • Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep
    Céline Ducournau, Nathalie Moiré, Rodolphe Carpentier, Pauline Cantin, Clément Herkt, Isabelle Lantier, Didier Betbeder, Isabelle Dimier-Poisson
    Frontiers in Immunology.2020;[Epub]     CrossRef
  • Status of common parasitic diseases in Korea in 2019
    Sun Huh
    Journal of the Korean Medical Association.2019; 62(8): 437.     CrossRef
  • The Association between Suicide Attempts andToxoplasma gondiiInfection
    Jeongjae Bak, Se-Hoon Shim, Young-Joon Kwon, Hwa-Young Lee, Ji Sun Kim, Heejung Yoon, Yeon Jung Lee
    Clinical Psychopharmacology and Neuroscience.2018; 16(1): 95.     CrossRef
  • TORCH (toxoplasmosis, rubella, cytomegalovirus, and herpes simplex virus) screening of small for gestational age and intrauterine growth restricted neonates: efficacy study in a single institute in Korea
    Mi Hae Chung, Chan Ok Shin, Juyoung Lee
    Korean Journal of Pediatrics.2018; 61(4): 114.     CrossRef
  • Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
    Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong
    The Korean Journal of Parasitology.2018; 56(5): 437.     CrossRef
  • Seroprevalence and Risk Factors of Toxoplasma gondii Infection among Cat Sitters in Korea
    Bong-Kwang Jung, Hyemi Song, Sang-Eun Lee, Min-Jae Kim, Jaeeun Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2017; 55(2): 203.     CrossRef
  • The 2015 Clinical Guidelines for the Treatment and Prevention of Opportunistic Infections in HIV-Infected Koreans: Guidelines for Opportunistic Infections

    Infection & Chemotherapy.2016; 48(1): 54.     CrossRef
  • High Toxoplasma gondii Seropositivity among Brain Tumor Patients in Korea
    Bong-Kwang Jung, Hyemi Song, Min-Jae Kim, Jaeeun Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2016; 54(2): 201.     CrossRef
  • Serological diagnosis of toxoplasmosis and standardization
    Kuo Zhang, Guigao Lin, Yanxi Han, Jinming Li
    Clinica Chimica Acta.2016; 461: 83.     CrossRef
  • Ocular Inflammation Associated with Systemic Infection
    Byung Gil Moon, Joo Yong Lee
    Hanyang Medical Reviews.2016; 36(3): 192.     CrossRef
  • Detection of Antibodies against Toxoplasma gondii in Cattle Raised in Gyeongbuk Province, Korea
    Jaejun Oh, Seung-Hun Lee, Seung-Jin Lee, Young-Hoan Kim, Seung-Chun Park, Man-Hee Rhee, Oh-Deog Kwon, Tae-Hwan Kim, Dongmi Kwak
    Journal of Food Protection.2016; 79(5): 821.     CrossRef
  • Prevalence ofToxoplasma gondiiandToxocara canisamong Patients with Uveitis
    Su Jin Lim, Sang Eun Lee, Sun Hyun Kim, Sung-Hee Hong, Yong Sung You, Oh Woong Kwon, Hyeun Seung Kim
    Ocular Immunology and Inflammation.2015; 23(2): 111.     CrossRef
  • Preliminary study on association between toxoplasmosis and breast cancer in Iran
    Narges Kalantari, Salman Ghaffari, Masomeh Bayani, Maryam Mitra Elmi, Daryush Moslemi, Novin Nikbakhsh, Fariedh Ghavipanjeh
    Asian Pacific Journal of Tropical Biomedicine.2015; 5(1): 44.     CrossRef
  • <i>Toxoplasma gondii</i> B1 Gene Detection in Feces of Stray Cats around Seoul, Korea and Genotype Analysis of Two Laboratory-Passaged Isolates
    Bong-Kwang Jung, Sang-Eun Lee, Hyemi Lim, Jaeeun Cho, Deok-Gyu Kim, Hyemi Song, Min-Jae Kim, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2015; 53(3): 259.     CrossRef
  • Prevalence ofToxoplasma gondiiandToxocara canisamong Patients with Uveitis
    Su Jin Lim, Sang Eun Lee, Sun Hyun Kim, Sung-Hee Hong, Yong Sung You, Oh Woong Kwon, Hyeun Seung Kim
    Ocular Immunology and Inflammation.2014; 22(5): 360.     CrossRef
  • Prominent IL-12 Production and Tumor Reduction in Athymic Nude Mice after Toxoplasma gondii Lysate Antigen Treatment
    Kyoung-Ho Pyo, Bong-Kwang Jung, Chun-Feng Xin, You-Won Lee, Jong-Yil Chai, Eun-Hee Shin
    The Korean Journal of Parasitology.2014; 52(6): 605.     CrossRef
  • Ocular toxoplasmosis past, present and new aspects of an old disease
    M. Maenz, D. Schlüter, O. Liesenfeld, G. Schares, U. Gross, U. Pleyer
    Progress in Retinal and Eye Research.2014; 39: 77.     CrossRef
  • Does the prevalence of latent toxoplasmosis and frequency of Rhesus-negative subjects correlate with the nationwide rate of traffic accidents?
    Jaroslav Flegr, Madhukar Dama
    Folia Parasitologica.2014; 61(6): 485.     CrossRef
  • Sex Bias in the Outcome of Human Tropical Infectious Diseases: Influence of Steroid Hormones
    H. Bernin, H. Lotter
    Journal of Infectious Diseases.2014; 209(suppl 3): S107.     CrossRef
  • Toxoplasmosis – A Global Threat. Correlation of Latent Toxoplasmosis with Specific Disease Burden in a Set of 88 Countries
    Jaroslav Flegr, Joseph Prandota, Michaela Sovičková, Zafar H. Israili, Delmiro Fernandez-Reyes
    PLoS ONE.2014; 9(3): e90203.     CrossRef
  • Chemotherapeutic drugs for common parasitic diseases in Korea
    Sun Huh
    Journal of the Korean Medical Association.2013; 56(6): 513.     CrossRef
  • 12,729 View
  • 106 Download
  • Crossref

Brief Communications

Production and Evaluation of Toxoplasma gondii Recombinant GRA7 for Serodiagnosis of Human Infections
Mina Selseleh, Hossein Keshavarz, Mehdi Mohebali, Saeedeh Shojaee, Monavar Selseleh, Mohammad Reza Eshragian, Fatemeh Mansouri, Mohammad Hossein Modarressi
Korean J Parasitol 2012;50(3):233-238.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.233

The precise diagnosis of the acute toxoplasmosis in pregnant women and immunocompromsied patients has critical importance. Most of the commercially available assays use the whole Toxoplasma soluble extract as the antigen. However, the assays currently available for the detection of specific anti-Toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. The aim of this study was production and evaluation of the usefulness of the recombinant Toxoplasma gondii GRA7 antigen for the serodiagnosis of Toxoplasma gondii IgM and IgG by ELISA. A total of 70 T. gondii IgM positive sera, 74 T. gondii IgG positive sera, and 60 sera from subjects who were not infected with T. gondii were examined. These sera were shown different absorbance values in ELISA test. To control the specificity of the rGRA7 other parasitic diseases, for example, echinococcosis, malaria, leishmaniasis, fascioliasis, and strongyloidiasis were tested of which none showed positive results. Sensitivity and specificity of the generated recombinant IgG ELISA in comparison with commercial ELISA (com ELISA) were 89% and 90%, and the sensitivity and specificity of the generated recombinant IgM ELISA were 96% and 90%, respectively. The results obtained here show that this antigen is useful for diagnostic purposes.

Citations

Citations to this article as recorded by  Crossref logo
  • IgM Antibody Detection as a Diagnostic Marker for Acute Toxoplasmosis: Current Status of Studies and Main Limitations
    Karolina Sołowińska, Lucyna Holec-Gąsior
    Antibodies.2025; 14(2): 44.     CrossRef
  • Design of a Multi-epitope Antigen for Toxoplasmosis Diagnosis: An Immunoinformatics Approach
    Negar Asadi, Elham Yousefi, Sadegh Feizollahzadeh, Mortaza Taheri-Anganeh, Shahram Khademvatan, Gordon S. Howarth
    Acta Parasitologica.2025;[Epub]     CrossRef
  • The Spread of Toxoplasmosis in Women within Al-Rifai District
    Rehab Issa Hashem Suhail, Shimaa Ahmed Mutab Nayef, Teeba Sabah Daham Mohammed, Yaqeen Mohammed Mahmud, Abdalrhman Falah Omar Ali
    European Journal of Medical and Health Research.2024; 2(4): 257.     CrossRef
  • Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection
    Min-ju Kim, Soeun J. Park, Hyunwoo Park
    European Journal of Medical Research.2024;[Epub]     CrossRef
  • Comparative Performance of Recombinant GRA6, GRA7, and GRA14 for the Serodetection of T. gondii Infection and Analysis of IgG Subclasses in Human Sera from the Philippines
    Rochelle Haidee Ybañez, Yoshifumi Nishikawa
    Pathogens.2022; 11(2): 277.     CrossRef
  • Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene
    Bahman Rahimi Esboei, Shirzad Fallahi, Mohammad Zarei, Bahram Kazemi, Mehdi Mohebali, Saeedeh Shojaee, Parisa Mousavi, Aref Teimouri, Raziyeh Mahmoudzadeh, Mirataollah Salabati, Hossein Keshavarz Valian
    BMC Infectious Diseases.2022;[Epub]     CrossRef
  • Four Chemotherapeutic Compounds That Limit Blood-Brain-Barrier Invasion by Toxoplasma gondii
    Zijing Yan, Hao Yuan, Junjie Wang, Zipeng Yang, Pian Zhang, Yasser S. Mahmmod, Xiaohu Wang, Tanghui Liu, Yining Song, Zhaowen Ren, Xiu-Xiang Zhang, Zi-Guo Yuan
    Molecules.2022; 27(17): 5572.     CrossRef
  • Assessment of an In-House Enzyme-Linked Immunosorbent Assay and IgG Avidity Test Based on SAG1 and GRA7 Proteins for Discriminating between Acute and Chronic Toxoplasmosis in Humans
    Aref Teimouri, Mohammad Javad Abbaszadeh Afshar, Sina Mohtasebi, Sanaz Jafarpour Azami, Rasoul Alimi, Hossein Keshavarz, Bobbi S. Pritt
    Journal of Clinical Microbiology.2021;[Epub]     CrossRef
  • Enhancing Immune Responses to a DNA Vaccine Encoding Toxoplasma gondii GRA7 Using Calcium Phosphate Nanoparticles as an Adjuvant
    Hong-Chao Sun, Jing Huang, Yuan Fu, Li-Li Hao, Xin Liu, Tuan-Yuan Shi
    Frontiers in Cellular and Infection Microbiology.2021;[Epub]     CrossRef
  • Designing Diagnostic Kit for Toxoplasma gondii Based on GRA7, SAG1, and ROP1 Antigens: An In Silico Strategy
    Amirreza Javadi Mamaghani, Seyyed Javad Seyyed Tabaei, Mohammad Mehdi Ranjbar, Ali Haghighi, Adel Spotin, Parisa Ataee Dizaji, Hanieh Rezaee
    International Journal of Peptide Research and Therapeutics.2020; 26(4): 2269.     CrossRef
  • Serological detection of T. gondii infection in humans using an immunochromatographic assay based on dense granule protein 7
    Rochelle Haidee D. Ybañez, Yoshifumi Nishikawa
    Parasitology International.2020; 76: 102089.     CrossRef
  • Detection of antibodies against Toxoplasma gondii in cats using an immunochromatographic test based on GRA7 antigen
    Rochelle Haidee D. YBAÑEZ, Hisako KYAN, Yoshifumi NISHIKAWA
    Journal of Veterinary Medical Science.2020; 82(4): 441.     CrossRef
  • Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans
    Rochelle Haidee D. Ybañez, Adrian P. Ybañez, Yoshifumi Nishikawa
    Frontiers in Cellular and Infection Microbiology.2020;[Epub]     CrossRef
  • Identification of novel antigens for serum IgG diagnosis of human toxoplasmosis
    Juntao Luo, Jingyi Wan, Ziru Tang, Shuang Shen
    Experimental Parasitology.2019; 204: 107722.     CrossRef
  • Candidate antigenic epitopes for vaccination and diagnosis strategies of Toxoplasma gondii infection: A review
    Amirreza Javadi Mamaghani, Anwar Fathollahi, Adel Spotin, Mohammad mehdi Ranjbar, Meisam Barati, Somayeh Aghamolaie, Maryam Karimi, Niloofar Taghipour, Mohammad Ashrafi, Seyyed Javad Seyyed Tabaei
    Microbial Pathogenesis.2019; 137: 103788.     CrossRef
  • Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection
    Ali Rostami, Panagiotis Karanis, Shirzad Fallahi
    Infection.2018; 46(3): 303.     CrossRef
  • Antigens to Detect the Acute Phase of Toxoplasmosis in Pregnant Women: Standardized Comparison
    Juan Gabriel Costa, María Julia Vilariño
    Biomarkers in Medicine.2018; 12(5): 517.     CrossRef
  • Detection of toxoplasma-specific immunoglobulin G in human sera: performance comparison of in house Dot-ELISA with ECLIA and ELISA
    Aref Teimouri, Mohammad Hossein Modarressi, Saeedeh Shojaee, Mehdi Mohebali, Nima Zouei, Mostafa Rezaian, Hossein Keshavarz
    European Journal of Clinical Microbiology & Infectious Diseases.2018; 37(8): 1421.     CrossRef
  • The association of latent toxoplasmosis and level of serum testosterone in humans
    Nima Zouei, Saeedeh Shojaee, Mehdi Mohebali, Hossein Keshavarz
    BMC Research Notes.2018;[Epub]     CrossRef
  • The relation of secondary sex ratio and miscarriage history with Toxoplasma gondii infection
    Saeedeh Shojaee, Aref Teimouri, Hossein Keshavarz, Sanaz Jafarpour Azami, Sahar Nouri
    BMC Infectious Diseases.2018;[Epub]     CrossRef
  • Seroprevalence of Toxoplasma gondii and Neospora caninum infections in cattle in Mongolia
    Baldorj Pagmadulam, Punsantsogvoo Myagmarsuren, Ragab M. Fereig, Makoto Igarashi, Naoaki Yokoyama, Badgar Battsetseg, Yoshifumi Nishikawa
    Veterinary Parasitology: Regional Studies and Reports.2018; 14: 11.     CrossRef
  • Early detection of Toxoplasma gondii infection by using a interferon gamma release assay: A review
    Shima Mahmoudi, Setareh Mamishi, Xun Suo, Hossein Keshavarz
    Experimental Parasitology.2017; 172: 39.     CrossRef
  • Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKCα
    Hyun-Jung Koh, Ye-Ram Kim, Jae-Sung Kim, Jin-Seung Yun, Kiseok Jang, Chul-Su Yang, Sarah M. Fortune
    PLOS Pathogens.2017; 13(1): e1006126.     CrossRef
  • Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo
    Prasanna Weeratunga, Thilina U. B. Herath, Tae-Hwan Kim, Hyun-Cheol Lee, Jae-Hoon Kim, Byeong-Hoon Lee, Eun-Seo Lee, Kiramage Chathuranga, W. A. Gayan Chathuranga, Chul-Su Yang, Jin Yeul Ma, Jong-Soo Lee
    Journal of Microbiology.2017; 55(11): 909.     CrossRef
  • Cloning and Sequencing of Truncated Toxoplasma gondii Subtilisin-Like 1 Antigen
    Ahmad Rouhizadeh, Ata A Ghadiri, Mohammad Razi Jalali, Masoud Ghorbanpour, Mohammad Hossein Razi Jalali
    Zahedan Journal of Research in Medical Sciences.2016;[Epub]     CrossRef
  • Toxoplasma gondii GRA7-Induced TRAF6 Activation Contributes to Host Protective Immunity
    Chul-Su Yang, Jae-Min Yuk, Young-Ha Lee, Eun-Kyeong Jo, J. H. Adams
    Infection and Immunity.2016; 84(1): 339.     CrossRef
  • Production and Evaluation of Recombinant Granule Antigen Protein GRA7 for Serodiagnosis ofToxoplasma gondiiInfection in Cattle
    Zedong Wang, Wei Ge, Jiping Li, Mingxing Song, Hongchao Sun, Feng Wei, Quan Liu
    Foodborne Pathogens and Disease.2014; 11(9): 734.     CrossRef
  • Toxoplasma gondii infection: What is the real situation?
    Waenurama Chemoh, Nongyao Sawangjaroen, Veeranoot Nissapatorn, Chitkasaem Suwanrath, Verapol Chandeying, Thanaporn Hortiwakul, Hemah Andiappan, Natthawan Sermwittayawong, Bunsri Charoenmak, Pisut Siripaitoon, Amorn Lekkla, Yaowalark Sukthana
    Experimental Parasitology.2013; 135(4): 685.     CrossRef
  • Toxoplasma gondii Recombinant Antigens as Tools for Serodiagnosis of Human Toxoplasmosis: Current Status of Studies
    Lucyna Holec-Gąsior
    Clinical and Vaccine Immunology.2013; 20(9): 1343.     CrossRef
  • 9,115 View
  • 95 Download
  • Crossref
Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR
Sang-Eun Lee, Sung-Hee Hong, Seong-Ho Lee, Young-Il Jeong, Su Jin Lim, Oh Woong Kwon, Sun Hyun Kim, Young Sung You, Shin-Hyeong Cho, Won-Ja Lee
Korean J Parasitol 2012;50(3):229-231.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.229

Toxoplasma gondii is a zoonotic parasite resulting in human infections and one of the infectious pathogens leading to uveitis and retinochoroiditis. The present study was performed to assess T. gondii infection in 20 ocular patients with chronic irregular recurrent uveitis (20 aqueous humor and 20 peripheral blood samples) using PCR. All samples were analyzed by nested PCR targeting a specific B1 gene of T. gondii. The PCR-positive rate was 25% (5/20), including 5% (1) in blood samples, 25% (5) in aqueous humor samples, and 5% (1) in both sample types. A molecular screening test for T. gondii infection in ocular patients with common clinical findings of an unclear retinal margin and an inflammatory membrane over the retina, as seen by fundus examination, may be helpful for early diagnosis and treatment.

Citations

Citations to this article as recorded by  Crossref logo
  • Molecular diagnosis of toxoplasmosis in immunocompromised patients
    Florence Robert-Gangneux, Sorya Belaz
    Current Opinion in Infectious Diseases.2016; 29(4): 330.     CrossRef
  • IgG Avidity Antibodies against Toxoplasma gondii in High Risk Females of Reproductive Age Group in India
    Naushaba Siddiqui, Fatima Shujatullah, Haris M. Khan, Tamkin Rabbani, Parvez A. Khan
    The Korean Journal of Parasitology.2014; 52(5): 487.     CrossRef
  • Establecimiento de una reacción en cadena de la polimerasa para la detección de bacterias y hongos
    Héctor Javier Pérez-Cano
    Revista Mexicana de Oftalmología.2014; 88(2): 67.     CrossRef
  • PCR-Based Detection of Toxoplasma gondii DNA in Blood and Ocular Samples for Diagnosis of Ocular Toxoplasmosis
    C. Bourdin, A. Busse, E. Kouamou, F. Touafek, B. Bodaghi, P. Le Hoang, D. Mazier, L. Paris, A. Fekkar, M. J. Loeffelholz
    Journal of Clinical Microbiology.2014; 52(11): 3987.     CrossRef
  • Prevalence and Genetic Characterization of Toxoplasma gondii in House Sparrows (Passer domesticus) in Lanzhou, China
    Wei Cong, Si-Yang Huang, Dong-Hui Zhou, Xiao-Xuan Zhang, Nian-Zhang Zhang, Quan Zhao, Xing-Quan Zhu
    The Korean Journal of Parasitology.2013; 51(3): 363.     CrossRef
  • Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
    Hyemi Lim, Sang-Eun Lee, Bong-Kwang Jung, Min-Ki Kim, Mi Youn Lee, Ho-Woo Nam, Jong-Gyun Shin, Cheong-Ha Yun, Han-Ik Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2012; 50(4): 287.     CrossRef
  • 9,743 View
  • 62 Download
  • Crossref
Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea
Hye-Jin Ahn, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam
Korean J Parasitol 2012;50(3):225-227.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.225

The seroepidemiological status of toxoplasmosis was surveyed among the residents of Cheorwon-gun, Gangwon-do by means of ELISA using a crude extract antigen of Toxoplasma gondii. The sera of 1,661 adult residents (866 males and 795 females) were collected and checked for IgG antibody titers, which showed 17.0% positive rate (282 sera). The positive rate was significantly different between the sex; 20.6% for males and 13.1% for females (P<0.05). The positive rates were higher in fifties of males (28.7%) and forties of females (20.0%). This positive rate of toxoplasmosis in Cheorwon-gun residents is regarded as the highest among the surveys of different geographical regions of Korea. This high positive rate may due in part to peculiar geographical locality of the surveyed area near the naturally well preserved demilitarized zone (DMZ) or presumably consumption of the pork imported from high endemic nations. Therefore, it is necessary to study further the epidemiology of toxoplasmosis in Cheorwon-gun.

Citations

Citations to this article as recorded by  Crossref logo
  • Clinical characteristics of toxoplasmosis patients in Korea: A retrospective study using health insurance review and assessment service data and electronic medical records
    Do-Won Ham, Bong-Kwang Jung, Ji-Hun Shin, Yong Joon Kim, Kyoung Yul Seo, Seung Mi Lee, Jae Hyoung Im, Jeong-Ran Kwon, Ho-Sung Lee, Kyung-Won Hwang, Eun-Hee Shin
    Parasites, Hosts and Diseases.2024; 62(4): 424.     CrossRef
  • Detection of Toxoplasma gondii oocysts in soil and risk mapping in an island environment in the Northeast of Brazil
    Pollyanne Raysa Fernandes de Oliveira, Renata Pimentel Bandeira de Melo, Umberto Diego Rodrigues de Oliveira, Fernando Jorge Rodrigues Magalhães, Rivaldo José Ferreira Junior, Müller Ribeiro Andrade, Rinaldo Aparecido Mota
    Transboundary and Emerging Diseases.2022; 69(6): 3457.     CrossRef
  • Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea
    Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(1): 9.     CrossRef
  • Clusters of Toxoplasmosis in Ganghwa-gun, Cheorwon-gun, and Goseong-gun, Korea
    Jihye Yu, Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(3): 251.     CrossRef
  • Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea
    Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho
    The Korean Journal of Parasitology.2020; 58(3): 257.     CrossRef
  • Clusters of Toxoplasmosis in Gyodong-Myeon and Samsan-Myeon, Ganghwa-Gun, Korea
    Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2020; 58(5): 493.     CrossRef
  • Seroprevalence of Toxoplasma gondii infection in cancer patients: A systematic review and meta-analysis
    Davood Anvari, Mehdi Sharif, Shahabeddin Sarvi, Sargis A. Aghayan, Shirzad Gholami, Abdol Sattar Pagheh, Seyed Abdollah Hosseini, Reza Saberi, Tooran Nayeri Chegeni, Zahra Hosseininejad, Ahmad Daryani
    Microbial Pathogenesis.2019; 129: 30.     CrossRef
  • Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
    Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong
    The Korean Journal of Parasitology.2018; 56(5): 437.     CrossRef
  • Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up
    Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(3): 247.     CrossRef
  • Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea
    Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(4): 385.     CrossRef
  • Seroprevalence of anti-Toxoplasma IgG and IgM among individuals who were referred to medical laboratories in Mazandaran province, northern Iran
    Mehdi Sharif, Ahmad Daryani, Zahra Ebrahimnejad, Shirzad Gholami, Ehsan Ahmadpour, Samaneh Borhani, Narges Lamsechi
    Journal of Infection and Public Health.2016; 9(1): 75.     CrossRef
  • Afatinib Reduces STAT6 Signaling of Host ARPE-19 Cells Infected with Toxoplasma gondii
    Zhaoshou Yang, Hye-Jin Ahn, Young-Hoon Park, Ho-Woo Nam
    The Korean Journal of Parasitology.2016; 54(1): 31.     CrossRef
  • First Report of Genotyping ofToxoplasma gondiiin Free-LivingMicrotus fortisin Northeastern China
    Xiao-Xuan Zhang, Si-Yang Huang, Ying-Guang Zhang, Yuan Zhang, Xing-Quan Zhu, Quan Liu
    Journal of Parasitology.2014; 100(5): 692.     CrossRef
  • Seroepidemiology of Toxoplasma gondii infection in drivers involved in road traffic accidents in the metropolitan area of Guadalajara, Jalisco, Mexico
    Ma de la Luz Galván-Ramírez, Laura Verónica Sánchez-Orozco, Laura Rocío Rodríguez, Saúl Rodríguez, Enrique Roig-Melo, Rogelio Troyo Sanromán, Erwin Chiquete, Juan Armendáriz-Borunda
    Parasites & Vectors.2013;[Epub]     CrossRef
  • Clinical Features and Treatment of Ocular Toxoplasmosis
    Young-Hoon Park, Ho-Woo Nam
    The Korean Journal of Parasitology.2013; 51(4): 393.     CrossRef
  • Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
    Hyemi Lim, Sang-Eun Lee, Bong-Kwang Jung, Min-Ki Kim, Mi Youn Lee, Ho-Woo Nam, Jong-Gyun Shin, Cheong-Ha Yun, Han-Ik Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2012; 50(4): 287.     CrossRef
  • A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea
    Zhaoshou Yang, Pyo Yun Cho, Seong Kyu Ahn, Hye-Jin Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam
    The Korean Journal of Parasitology.2012; 50(3): 191.     CrossRef
  • 9,511 View
  • 93 Download
  • Crossref

Original Articles

Real-Time RT-PCR on SAG1 and BAG1 Gene Expression during Stage Conversion in Immunosuppressed Mice Infected with Toxoplasma gondii Tehran Strain
Monavar Selseleh, Mohammad Hossein Modarressi, Mehdi Mohebali, Saeedeh Shojaee, Mohammad Reza Eshragian, Mina Selseleh, Ebrahim Azizi, Hossein Keshavarz
Korean J Parasitol 2012;50(3):199-205.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.199

Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it is difficult to discriminate between these 2 stages. Toxoplasma PCR assays using gDNA as a template have been unable to discriminate between an increase or decrease in SAG1 and BAG1 expression between the active tachyzoite stage and the latent bradyzoite stage. In the present study, real-time RT-PCR assay was used to detect the expression of bradyzoite (BAG1)- and tachyzoite-specific genes (SAG1) during bradyzoite/tachyzoite stage conversion in mice infected with T. gondii Tehran strain after dexamethasone sodium phosphate (DXM) administration. The conversion reaction was observed in the lungs and brain tissues of experimental mice, indicated by SAG1 expression at day 6 after DXM administration, and continued until day 14. Bradyzoites were also detected in both organs throughout the study; however, it decreased at day 14 significantly. It is suggested that during the reactivation period, bradyzoites not only escape from the cysts and reinvade neighboring cells as tachyzoites, but also converted to new bradyzoites. In summary, the real-time RT-PCR assay provided a reliable, fast, and quantitative way of detecting T. gondii reactivation in an animal model. Thus, this method may be useful for diagnosing stage conversion in clinical specimens of immunocompromised patients (HIV or transplant patients) for early identification of tachyzoite-bradyzoite stage conversion.

Citations

Citations to this article as recorded by  Crossref logo
  • Human Retinal Organoid Model of Ocular Toxoplasmosis
    Liam M. Ashander, Grace E. Lidgerwood, Amanda L. Lumsden, João M. Furtado, Alice Pébay, Justine R. Smith
    Pathogens.2025; 14(3): 286.     CrossRef
  • Comprehensive diagnostic approaches to feline toxoplasmosis: Bridging traditional methods and emerging technologies
    Dan Zhao, Yanzhen Liao, Hao Liu, Jianwei Wang, Ruiying Liang, Rongqiong Zhou, Jiabo Ding, Sixin Zhang, Xinming Tang
    Virulence.2025;[Epub]     CrossRef
  • High Efficacy of Green Synthesized Silver Nanoparticles for Treatment of Toxoplasma Gondii Infection Through Their Immunomodulatory, Anti-Inflammatory, and Antioxidant Potency
    Qais A.H. Majeed, Sultan F. Alnomasy, Abdullah F. Shater, Abdullah D. Alanazi
    Acta Parasitologica.2024; 69(2): 1201.     CrossRef
  • Diagnosis of Toxoplasmosis Using Surface Antigen Grade 1 Detection by ELISA, Nano-Gold ELISA, and PCR in Pregnant Women
    Nagwa SM Aly, Hye-Sook Kim, Yasmin M Marei, Azza S Elhamshary, Ibrahim R Bayoumi, Rabab E Omar, Dina A Mohammed, Shin-Ichi Miyoshi, Gehan A Rashed
    International Journal of Nanomedicine.2023; Volume 18: 1335.     CrossRef
  • Assessment of tissue levels of miR-146a and proinflammatory cytokines in experimental cerebral toxoplasmosis following atovaquone and clindamycin treatment: An in vivo study
    Nima Zouei, Abdolhossein Dalimi, Majid Pirestani, Fatemeh Ghaffarifar
    Microbial Pathogenesis.2023; 184: 106340.     CrossRef
  • Detection of Toxoplasma gondii oocysts on organic and conventionally grown produce
    Emily L. Lilly, Nathan J. Webster
    Food Microbiology.2021; 99: 103798.     CrossRef
  • Temporal expression of Toxoplasma stage-specific genes in brain tissue: coincidence with parasitological and histopathological findings in mice models
    Mona H. El-Sayad, Neveen A. Hussein, A. H. Kazem, Omnya A. El Geddawi, Enas M. Rizk, Hend A. El-Taweel
    Parasitology Research.2020; 119(7): 2299.     CrossRef
  • Micronemal protein 13 contributes to the optimal growth of Toxoplasma gondii under stress conditions
    Shu Ye, Ningbo Xia, Pengfei Zhao, Jichao Yang, Yanqin Zhou, Bang Shen, Junlong Zhao
    Parasitology Research.2019; 118(3): 935.     CrossRef
  • Nanoemulsion of atovaquone as a promising approach for treatment of acute and chronic toxoplasmosis
    Sanaz Jafarpour Azami, Amir Amani, Hossein Keshavarz, Roqya Najafi-Taher, Mehdi Mohebali, Mohammad Ali Faramarzi, Mahmood Mahmoudi, Saeedeh Shojaee
    European Journal of Pharmaceutical Sciences.2018; 117: 138.     CrossRef
  • ANK1 and DnaK-TPR, Two Tetratricopeptide Repeat-Containing Proteins Primarily Expressed in Toxoplasma Bradyzoites, Do Not Contribute to Bradyzoite Differentiation
    Jichao Yang, Lihong Zhang, Huiyan Diao, Ningbo Xia, Yanqin Zhou, Junlong Zhao, Bang Shen
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • Deletion of mitogen-activated protein kinase 1 inhibits development and growth of Toxoplasma gondii
    Lili Cao, Zedong Wang, Shuchao Wang, Jiping Li, Xinglong Wang, Feng Wei, Quan Liu
    Parasitology Research.2016; 115(2): 797.     CrossRef
  • Brain cystogenesis capacity of Toxoplasma gondii, avirulent Tehran strain in mice
    Mehrzad Saraei, Yosef Ghaderi, Tahereh Mosavi, Mojtaba Shahnazi, Hossein Keshavarz, Saeedeh Shojaee
    Asian Pacific Journal of Tropical Disease.2014; 4: S739.     CrossRef
  • 10,233 View
  • 105 Download
  • Crossref
A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea
Zhaoshou Yang, Pyo Yun Cho, Seong Kyu Ahn, Hye-Jin Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam
Korean J Parasitol 2012;50(3):191-197.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.191

Seroepidemiological changes of Toxoplasma gondii infection among the residents of the islands of Gangwha-gun, Incheon for 2 years were surveyed and evaluated by ELISA using a crude extract antigen. In 2010, sera of 919 adult residents in Gyodong-myeon and 313 adults in Samsan-myeon were collected and checked for IgG antibody titers, which showed 14.5% (133 sera) and 19.8% (62 sera) positive rates, respectively. In 2011, sera of 955 adults in Gyodong-myeon and 341 adults in Samsan-myeon were examined, which showed an increase of positive rates to 23.8% (227 sera) and 31.7% (108 sera), respectively. Totally, the seroprevalence of the first year was 15.8% and it increased rapidly to 25.8% in the second year. The positive rates of both sexes increased simultaneously with the significant ratio of males to females by 1.7-2.2 fold (P<0.05). In both myeons, 661 sera were collected every year and showed changes in optical density (OD) in 177 sera; newly found as positives in 73 persons (11.0%), negative conversion in 10 persons (1.5%), and maintained or increased in 94 persons (14.2%). This rapid increase in the prevalence of toxoplasmosis in Gangwha islands may be due to in part peculiar changes in the toxoplasmic environment of the islands and presumably the consumption of the pork bred domestically within the islands or imported from high endemic nations. It is necessary to find out symptomatic toxoplasmic patients and confirm the risk factors for further infection in the islands of Gangwha-gun.

Citations

Citations to this article as recorded by  Crossref logo
  • Is the incidence of congenital toxoplasmosis declining?
    Gregory Colin Milne, Joanne P. Webster, Martin Walker
    Trends in Parasitology.2023; 39(1): 26.     CrossRef
  • Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea
    Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(1): 9.     CrossRef
  • Cross-sectional survey for Toxoplasma gondii infection in humans in Fernando de Noronha island, Brazil
    Maria da Conceição Carvalho, Müller Ribeiro-Andrade, Renata Pimentel Bandeira de Melo, Dandara Matias Guedes, José Wilton Pinheiro Junior, Erika Fernanda Torres Samico Fernandes Cavalcanti, Fernando Jorge Rodrigues Magalhães, Rinaldo Aparecido Mota
    Revista Brasileira de Parasitologia Veterinária.2021;[Epub]     CrossRef
  • Status of common parasitic diseases in Korea in 2019
    Sun Huh
    Journal of the Korean Medical Association.2019; 62(8): 437.     CrossRef
  • TORCH (toxoplasmosis, rubella, cytomegalovirus, and herpes simplex virus) screening of small for gestational age and intrauterine growth restricted neonates: efficacy study in a single institute in Korea
    Mi Hae Chung, Chan Ok Shin, Juyoung Lee
    Korean Journal of Pediatrics.2018; 61(4): 114.     CrossRef
  • Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
    Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong
    The Korean Journal of Parasitology.2018; 56(5): 437.     CrossRef
  • Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up
    Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(3): 247.     CrossRef
  • Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea
    Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(4): 385.     CrossRef
  • Chronic Toxoplasmosis Modulates the Induction of Contact Hypersensitivity by TNCB in Mouse Model
    Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2015; 53(6): 755.     CrossRef
  • Mesial Temporal Lobe Epilepsy in Congenital Toxoplasmosis: A Case Report
    Woo Kyo Jeong, Byung-Euk Joo, Ji-hye Seo, Jun Kyu Mun, Juhyeon Kim, Dae-Won Seo
    Journal of Epilepsy Research.2015; 5(1): 25.     CrossRef
  • Prevalence and Genetic Characterization of Toxoplasma gondii in House Sparrows (Passer domesticus) in Lanzhou, China
    Wei Cong, Si-Yang Huang, Dong-Hui Zhou, Xiao-Xuan Zhang, Nian-Zhang Zhang, Quan Zhao, Xing-Quan Zhu
    The Korean Journal of Parasitology.2013; 51(3): 363.     CrossRef
  • Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
    Hyemi Lim, Sang-Eun Lee, Bong-Kwang Jung, Min-Ki Kim, Mi Youn Lee, Ho-Woo Nam, Jong-Gyun Shin, Cheong-Ha Yun, Han-Ik Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2012; 50(4): 287.     CrossRef
  • 9,644 View
  • 78 Download
  • Crossref
IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans
Amir Hossien Rahbari, Hossien Keshavarz, Saeedeh Shojaee, Mehdi Mohebali, Mostafa Rezaeian
Korean J Parasitol 2012;50(2):99-102.
Published online May 24, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.2.99

Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI≤50), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.

Citations

Citations to this article as recorded by  Crossref logo
  • SELEX-derived DNA aptamer utilized for sensitive electrochemical biosensing of Toxoplasma gondii surface antigen 1
    Pandiyaraj Kanagavalli, Shahad Alkhaldi, Mohammed Zourob, Shimaa Eissa
    International Journal of Biological Macromolecules.2025; 310: 143530.     CrossRef
  • Comparison of B1 and RE 529 gene targets by real time PCR and LAMP assay for diagnosis of toxoplasmosis in pregnant females
    Priya Datta, Puja Garg, Divya Rattan, Rashmi Bagga, Minakshi Rohilla, Sumeeta Khurana, Rakesh Sehgal
    Indian Journal of Medical Microbiology.2024; 47: 100481.     CrossRef
  • Formulation and evaluation of atovaquone-loaded macrophage-derived exosomes against Toxoplasma gondii : in vitro and in vivo assessment
    Fatemeh Goudarzi, Vahid Jajarmi, Saeedeh Shojaee, Mehdi Mohebali, Hossein Keshavarz, Emma H. Wilson
    Microbiology Spectrum.2024;[Epub]     CrossRef
  • Role of IgG avidity in eyes with active Toxoplasma retinochoroiditis
    Pratik Lakhmawar, Anup Kelgaonkar, Anamika Patel, Ashish Khalsa, Soumyava Basu
    Indian Journal of Ophthalmology.2024; 72(Suppl 4): S601.     CrossRef
  • Seroprevalence and Risk Factors of Toxoplasmosis in Female Students from Northwest Iran
    Mehrdad Rostami, Sanam Rezaei Benam, Elham Sadat Roointan, Arezoo Bozorgomid, Saber Raeghi, Reza Shafiei
    Journal of Kerman University of Medical Sciences.2024; 31(3): 153.     CrossRef
  • Congenital Toxoplasmosis Diagnosis: Current Approaches and New Insights
    Alfredo Márquez-Mauricio, Heriberto Caballero-Ortega, Fernando Gómez-Chávez
    Acta Parasitologica.2023; 68(3): 473.     CrossRef
  • Diagnosis of Acute Toxoplasmosis in the First Trimester of Pregnancy by IgG Avidity
    Afshin Azimi, Mahdi Parsaei, Eissa Soleymani, Elham Mehranzadeh, Amir Hossein Maghsood
    Avicenna Journal of Clinical Medicine.2022; 29(1): 50.     CrossRef
  • Recombinant AMA1 Virus-like Particle Antigen for Serodiagnosis of Toxoplasma gondii Infection
    Min-Ju Kim, Ki-Back Chu, Jie Mao, Hae-Ji Kang, Gi-Deok Eom, Keon-Woong Yoon, Su-Hwa Lee, Eun-Kyung Moon, Young-Ha Lee, Fu-Shi Quan
    Biomedicines.2022; 10(11): 2812.     CrossRef
  • Dissociating direct and indirect effects: a theoretical framework of how latent toxoplasmosis affects cognitive profile across the lifespan
    Lorenza Colzato, Wenxin Zhang, Christian Beste, Ann-Kathrin Stock
    Neurobiology of Aging.2021; 102: 119.     CrossRef
  • Modified ELISA for antibody avidity evaluation: The need for standardization
    Victor Araujo Correa, Thais Sousa Rodrigues, Amanda Izeli Portilho, Gabriela Trzewikoswki de Lima, Elizabeth De Gaspari
    Biomedical Journal.2021; 44(4): 433.     CrossRef
  • IgG Avidity in Samples Collected on Filter Paper: Importance of The Early Diagnosis of Congenital Toxoplasmosis
    Jéssica Yonara de Souza, Taynara Cristina Gomes, Hanstter Hallison Alves Rezende, Heloisa Ribeiro Storchilo, Patrícia Giffron Rodrigues, Ana Maria de Castro
    Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics.2021; 43(12): 887.     CrossRef
  • Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans
    Rochelle Haidee D. Ybañez, Adrian P. Ybañez, Yoshifumi Nishikawa
    Frontiers in Cellular and Infection Microbiology.2020;[Epub]     CrossRef
  • Diagnostic significance of C-reactive protein and hematological parameters in acute toxoplasmosis
    Vanessa Sandri, Itamar Luís Gonçalves, Gustavo Machado das Neves, Marisa Lucia Romani Paraboni
    Journal of Parasitic Diseases.2020; 44(4): 785.     CrossRef
  • Evaluation of Toxoplasma gondii propagated in specific pathogen free embryonated chicken egg, for diagnosis of toxoplasmosis in equids and human
    Abeer M. Abdalhamed, Mohey A. Hassanain, Gamil S. G. Zeedan, Raafat M. Shaapan
    Journal of Parasitic Diseases.2019; 43(3): 498.     CrossRef
  • Serologic Tests of IgG and IgM Antibodies and IgG Avidity for Diagnosis of Ocular Toxoplasmosis
    Bahman Rahimi-Esboei, Mohammad Zarei, Mehdi Mohebali, Hossein Keshavarz Valian, Saeedeh Shojaee, Raziyeh Mahmoudzadeh, Mirataollah Salabati
    The Korean Journal of Parasitology.2018; 56(2): 147.     CrossRef
  • Dynamics of Immune Responses during ExperimentalMycobacterium kansasiiInfection of Cynomolgus Monkeys(Macaca fascicularis)
    Fangui Min, Lifang He, Yinzhu Luo, Shuwu Huang, Jinchun Pan, Jing Wang, Ruike Wu, Lan Zhang, Meili Chen
    Mediators of Inflammation.2018; 2018: 1.     CrossRef
  • Detection of Toxoplasma gondii B1 gene in placenta does not prove congenital toxoplasmosis
    Khosro Sardarian, Amir Hossein Maghsood, Marzieh Farimani, Mehrdad Hajiloii, Massoud Saidijam, Manizheh Farahpour, Hanieh Mahaki, Alireza Zamani
    Human Antibodies.2018; 27(1): 31.     CrossRef
  • Evaluation of Toxoplasma gondii soluble, whole and excretory/secretary antigens for diagnosis of toxoplasmosis by ELISA test
    S. Pishkari, S. Shojaee, H. Keshavarz, M. Salimi, M. Mohebali
    Journal of Parasitic Diseases.2017; 41(1): 289.     CrossRef
  • Prevalence and Risk Factors for Toxoplasmosis in Middle Java, Indonesia
    Annisa Retmanasari, Barandi Sapta Widartono, Mahardika Agus Wijayanti, Wayan Tunas Artama
    EcoHealth.2017; 14(1): 162.     CrossRef
  • Prenatal toxoplasmosis antibody and childhood autism
    Marisa N. Spann, Andre Sourander, Heljä‐Marja Surcel, Susanna Hinkka‐Yli‐Salomäki, Alan S. Brown
    Autism Research.2017; 10(5): 769.     CrossRef
  • Early detection of Toxoplasma gondii infection by using a interferon gamma release assay: A review
    Shima Mahmoudi, Setareh Mamishi, Xun Suo, Hossein Keshavarz
    Experimental Parasitology.2017; 172: 39.     CrossRef
  • T cell immunoregulation in active ocular toxoplasmosis
    Cynthia A. Cordeiro, Erica L.M. Vieira, Vinicius M. Castro, Walderez O. Dutra, Rogerio A. Costa, Juliana L. Orefice, Wesley R. Campos, Fernando Orefice, Lucy H. Young, Antonio Lucio Teixeira
    Immunology Letters.2017; 184: 84.     CrossRef
  • Inactivated H7 Influenza Virus Vaccines Protect Mice despite Inducing Only Low Levels of Neutralizing Antibodies
    Ram P. Kamal, Kristy Blanchfield, Jessica A. Belser, Nedzad Music, Wen-Pin Tzeng, Crystal Holiday, Ashley Burroughs, Xiangjie Sun, Taronna R. Maines, Min Z. Levine, Ian A. York, Rebecca Ellis Dutch
    Journal of Virology.2017;[Epub]     CrossRef
  • Diagnosis of acute toxoplasmosis in pregnant women referred to therapeutic centers of Alborz Province (Iran) using immunoglobulin G avidity ELISA technique
    Lame Akhlaghi, Fatemeh Tabatabaie, Ramtin Hadighi, Fatemeh Maleki, Fateme Hajialiani, Mohammad Saaid Dayer, Abbas Ghasemi, Masoud Roudbari
    Asian Pacific Journal of Tropical Disease.2016; 6(11): 864.     CrossRef
  • Prevalence of toxoplasmosis in pregnant women and vertical transmission of Toxoplasma gondii in patients from basic units of health from Gurupi, Tocantins, Brazil, from 2012 to 2014
    Marcos Gontijo da Silva, Marina Clare Vinaud, Ana Maria de Castro, Ira J Blader
    PLOS ONE.2015; 10(11): e0141700.     CrossRef
  • Recombinant influenza H7 hemagglutinins induce lower neutralizing antibody titers in mice than do seasonal hemagglutinins
    Kristy Blanchfield, Ram P. Kamal, Wen‐Pin Tzeng, Nedzad Music, Jason R. Wilson, James Stevens, Aleksander S. Lipatov, Jacqueline M. Katz, Ian A. York
    Influenza and Other Respiratory Viruses.2014; 8(6): 628.     CrossRef
  • Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis
    Elizandra Roselaine Schoenardie, Carlos James Scaini, Luciana Farias da Costa de Avila, Rita Leal Sperotto, Sibele Borsuk, Cristine Dias Pires Felicetti, Michele Pepe, Maria Elisabeth Aires Berne
    Revista Brasileira de Parasitologia Veterinária.2014; 23(3): 403.     CrossRef
  • Dual-Aptamer-Based Biosensing of Toxoplasma Antibody
    Yang Luo, Xing Liu, Tianlun Jiang, Pu Liao, Weiling Fu
    Analytical Chemistry.2013; 85(17): 8354.     CrossRef
  • Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting
    Siamak Ali-Heydari, Hossien Keshavarz, Saeedeh Shojaee, Mehdi Mohebali
    Parasite.2013; 20: 18.     CrossRef
  • 12,949 View
  • 133 Download
  • Crossref
Gene Expression Profiles in Genetically Different Mice Infected with Toxoplasma gondii: ALDH1A2, BEX2, EGR2, CCL3 and PLAU
Hassan Ahmed Hassan Ahmed Ismail, Juan-Hua Quan, Zhou Wei, In-Wook Choi, Guang-Ho Cha, Dae-Whan Shin, Young-Ha Lee, Chang-June Song
Korean J Parasitol 2012;50(1):7-13.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.7

Toxoplasma gondii can modulate host cell gene expression; however, determining gene expression levels in intermediate hosts after T. gondii infection is not known much. We selected 5 genes (ALDH1A2, BEX2, CCL3, EGR2 and PLAU) and compared the mRNA expression levels in the spleen, liver, lung and small intestine of genetically different mice infected with T. gondii. ALDH1A2 mRNA expressions of both mouse strains were markedly increased at day 1-4 postinfection (PI) and then decreased, and its expressions in the spleen and lung were significantly higher in C57BL/6 mice than those of BALB/c mice. BEX2 and CCR3 mRNA expressions of both mouse strains were significantly increased from day 7 PI and peaked at day 15-30 PI (P<0.05), especially high in the spleen liver or small intestine of C57BL/6 mice. EGR2 and PLAU mRNA expressions of both mouse strains were significantly increased after infection, especially high in the spleen and liver. However, their expression patterns were varied depending on the tissue and mouse strain. Taken together, T. gondii-susceptible C57BL/6 mice expressed higher levels of these 5 genes than did T. gondii-resistant BALB/c mice, particularly in the spleen and liver. And ALDH1A2 and PLAU expressions were increased acutely, whereas BEX2, CCL3 and EGR2 expressions were increased lately. Thus, these demonstrate that host genetic factors exert a strong impact on the expression of these 5 genes and their expression patterns were varied depending on the gene or tissue.

Citations

Citations to this article as recorded by  Crossref logo
  • Effect of Puerarin, Baicalin and Berberine Hydrochloride on the Regulation of IPEC-J2 Cells Infected with Enterotoxigenic Escherichia coli
    Xiaoxi Liu, Fenghua Liu, Yunfei Ma, Huanrong Li, Xianghong Ju, Jianqin Xu
    Evidence-Based Complementary and Alternative Medicine.2019; 2019: 1.     CrossRef
  • Gene Silencing of Porcine MUC13 and ITGB5: Candidate Genes towards Escherichia coli F4ac Adhesion
    Chuanli Zhou, Zhengzhu Liu, Yang Liu, Weixuan Fu, Xiangdong Ding, Jianfeng Liu, Ying Yu, Qin Zhang, Lucia R. Languino
    PLoS ONE.2013; 8(7): e70303.     CrossRef
  • 10,059 View
  • 86 Download
  • Crossref
Anti-Apoptotic Effects of SERPIN B3 and B4 via STAT6 Activation in Macrophages after Infection with Toxoplasma gondii
Kyoung Ju Song, Hye-Jin Ahn, Ho-Woo Nam
Korean J Parasitol 2012;50(1):1-6.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.1

Toxoplasma gondii penetrates all kinds of nucleated eukaryotic cells but modulates host cells differently for its intracellular survival. In a previous study, we found out that serine protease inhibitors B3 and B4 (SERPIN B3/B4 because of their very high homology) were significantly induced in THP-1-derived macrophages infected with T. gondii through activation of STAT6. In this study, to evaluate the effects of the induced SERPIN B3/B4 on the apoptosis of T. gondii-infected THP-1 cells, we designed and tested various small interfering (si-) RNAs of SERPIN B3 or B4 in staurosporine-induced apoptosis of THP-1 cells. Anti-apoptotic characteristics of THP-1 cells after infection with T. gondii disappeared when SERPIN B3/B4 were knock-downed with gene specific si-RNAs transfected into THP-1 cells as detected by the cleaved caspase 3, poly-ADP ribose polymerase and DNA fragmentation. This anti-apoptotic effect was confirmed in SERPIN B3/B4 overexpressed HeLa cells. We also investigated whether inhibition of STAT6 affects the function of SERPIN B3/B4, and vice versa. Inhibition of SERPIN B3/B4 did not influence STAT6 expression but SERPIN B3/B4 expression was inhibited by STAT6 si-RNA transfection, which confirmed that SERPIN B3/B4 was induced under the control of STAT6 activation. These results suggest that T. gondii induces SERPIN B3/B4 expression via STAT6 activation to inhibit the apoptosis of infected THP-1 cells for longer survival of the intracellular parasites themselves.

Citations

Citations to this article as recorded by  Crossref logo
  • Identification and functional analysis of a serine protease inhibitor using machine learning strategy
    Heqian Zhang, Yaxin Wu, Yanran Zhu, Liangjun Ge, Jiaquan Huang, Zhiwei Qin
    International Journal of Biological Macromolecules.2024; 265: 130852.     CrossRef
  • Overview of Apoptosis, Autophagy, and Inflammatory Processes in Toxoplasma gondii Infected Cells
    Ehsan Ahmadpour, Farhad Babaie, Tohid Kazemi, Sirous Mehrani Moghaddam, Ata Moghimi, Ramin Hosseinzadeh, Veeranoot Nissapatorn, Abdol Sattar Pagheh
    Pathogens.2023; 12(2): 253.     CrossRef
  • Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains
    Melissa Martins Oliveira, Camila Ramalho Bonturi, Bruno Ramos Salu, Maria Luiza Vilela Oliva, Renato Arruda Mortara, Cristina Mary Orikaza
    Frontiers in Immunology.2022;[Epub]     CrossRef
  • BEWO trophoblast cells and Toxoplasma gondii infection modulate cell death mechanisms in THP-1 monocyte cells by interference in the expression of death receptor and intracellular proteins
    Andressa da Silva Castro, Mariana Bodini Angeloni, Bellisa de Freitas Barbosa, Renata Lima de Miranda, Samuel Cota Teixeira, Pâmela Mendonça Guirelli, Fernanda Chaves de Oliveira, Rafaela José da Silva, Priscila Silva Franco, Mayara Ribeiro, Iliana Claudi
    Tissue and Cell.2021; 73: 101658.     CrossRef
  • Transcriptional profiling of human macrophages during infection withBordetella pertussis
    Denisa Petráčková, Mariam R. Farman, Fabian Amman, Irena Linhartová, Ana Dienstbier, Dilip Kumar, Jakub Držmíšek, Ivo Hofacker, Maria Eugenia Rodriguez, Branislav Večerek
    RNA Biology.2020; 17(5): 731.     CrossRef
  • Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors
    Hye-Jung Kim, Hye-Jin Ahn, Hyeweon Kang, Jaehui Park, Seul gi Oh, Saehae Choi, Won-Kyu Lee, Ho-Woo Nam
    The Korean Journal of Parasitology.2020; 58(3): 249.     CrossRef
  • Toxoplasma gondiiModulates the Host Cell Responses: An Overview of Apoptosis Pathways
    Nour Mammari, Mohamad Adnan Halabi, Souha Yaacoub, Hilda Chlala, Marie-Laure Dardé, Bertrand Courtioux
    BioMed Research International.2019; 2019: 1.     CrossRef
  • Mechanisms of Human Innate Immune Evasion by Toxoplasma gondii
    Tatiane S. Lima, Melissa B. Lodoen
    Frontiers in Cellular and Infection Microbiology.2019;[Epub]     CrossRef
  • Strategies Developed by Toxoplasma gondii to Survive in the Host
    Wanbo Zhu, Jingyang Li, Faustina Pappoe, Jilong Shen, Li Yu
    Frontiers in Microbiology.2019;[Epub]     CrossRef
  • Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella
    Ling Lv, Bing Huang, Qiping Zhao, Zongping Zhao, Hui Dong, Shunhai Zhu, Ting Chen, Ming Yan, Hongyu Han
    Parasites & Vectors.2018;[Epub]     CrossRef
  • Quantitative proteomic analysis of host epithelial cells infected by Salmonella enterica serovar Typhimurium
    Linlu Qi, Mo Hu, Jiaqi Fu, Yanhua Liu, Mei Wu, Kaiwen Yu, Xiaoyun Liu
    PROTEOMICS.2017;[Epub]     CrossRef
  • Phosphoproteome of Toxoplasma gondii Infected Host Cells Reveals Specific Cellular Processes Predominating in Different Phases of Infection
    Hai-Xia Wei, Ai-Yuan Chen, Cheng He, Hong-Juan Peng, Xiao-Shuang Feng
    The American Journal of Tropical Medicine and Hygiene.2017; 97(1): 236.     CrossRef
  • Host-Toxoplasma gondii Coadaptation Leads to Fine Tuning of the Immune Response
    Thaís Rigueti Brasil, Celio Geraldo Freire-de-Lima, Alexandre Morrot, Andrea Cristina Vetö Arnholdt
    Frontiers in Immunology.2017;[Epub]     CrossRef
  • Interferon-γ Protects from Staphylococcal Alpha Toxin-Induced Keratinocyte Death through Apolipoprotein L1
    Anne M. Brauweiler, Elena Goleva, Donald Y.M. Leung
    Journal of Investigative Dermatology.2016; 136(3): 658.     CrossRef
  • SERPINB3/B4 Contributes to Early Inflammation and Barrier Dysfunction in an Experimental Murine Model of Atopic Dermatitis
    Umasundari Sivaprasad, Kayla G. Kinker, Mark B. Ericksen, Mark Lindsey, Aaron M. Gibson, Stacey A. Bass, Nicolas S. Hershey, Jingyuan Deng, Mario Medvedovic, Gurjit K. Khurana Hershey
    Journal of Investigative Dermatology.2015; 135(1): 160.     CrossRef
  • Trophoblast-macrophage crosstalk on human extravillous under Toxoplasma gondii infection
    P.M. Guirelli, M.B. Angeloni, B.F. Barbosa, A.O. Gomes, A.S. Castro, P.S. Franco, R.J. Silva, J.G. Oliveira, O.A. Martins-Filho, J.R. Mineo, F. Ietta, E.A. Ferro
    Placenta.2015; 36(10): 1106.     CrossRef
  • Toxoplasma gondii inhibits apoptosis via a novel STAT3-miR-17–92-Bim pathway in macrophages
    Yihong Cai, He Chen, Xuwei Mo, Yuanyuan Tang, Xiucai Xu, Aimei Zhang, Zhaorong Lun, Fangli Lu, Yong Wang, Jilong Shen
    Cellular Signalling.2014; 26(6): 1204.     CrossRef
  • The role of STAT-6 as a key transcription regulator in HeLa cell death induced by IFN-γ/TNF-α co-immobilized on nanoparticles
    Zhibin Li, Yan-Qing Guan, Jun-Ming Liu
    Biomaterials.2014; 35(18): 5016.     CrossRef
  • Adaptive Evolution and Divergence of SERPINB3: A Young Duplicate in Great Apes
    Sílvia Gomes, Patrícia I. Marques, Rune Matthiesen, Susana Seixas, Charaf Benarafa
    PLoS ONE.2014; 9(8): e104935.     CrossRef
  • Risk factors associated with seroprevalence of Neospora caninum in dogs from urban and rural areas of milk and coffee production in Minas Gerais state, Brazil
    C. I. NOGUEIRA, L. P. MESQUITA, C. C. ABREU, K. Y. R. NAKAGAKI, J. N. SEIXAS, P. S. BEZERRA, C. M. B. M. ROCHA, A. M. GUIMARAES, A. P. PECONICK, M. S. VARASCHIN
    Epidemiology and Infection.2013; 141(11): 2286.     CrossRef
  • In-depth proteomic analysis of the human cerumen—A potential novel diagnostically relevant biofluid
    Martin Andreas Feig, Elke Hammer, Uwe Völker, Nico Jehmlich
    Journal of Proteomics.2013; 83: 119.     CrossRef
  • Serpins, Immunity and Autoimmunity: Old Molecules, New Functions
    Mariele Gatto, Luca Iaccarino, Anna Ghirardello, Nicola Bassi, Patrizia Pontisso, Leonardo Punzi, Yehuda Shoenfeld, Andrea Doria
    Clinical Reviews in Allergy & Immunology.2013; 45(2): 267.     CrossRef
  • STAT3-dependent transactivation of miRNA genes following Toxoplasma gondii infection in macrophage
    Yihong Cai, He Chen, Lei Jin, Yibo You, Jilong Shen
    Parasites & Vectors.2013;[Epub]     CrossRef
  • Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression
    M.B. Angeloni, P.M. Guirelli, P.S. Franco, B.F. Barbosa, A.O. Gomes, A.S. Castro, N.M. Silva, O.A. Martins-Filho, T.W.P. Mineo, D.A.O. Silva, J.R. Mineo, E.A.V. Ferro
    Placenta.2013; 34(11): 973.     CrossRef
  • Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8+ cytotoxic T lymphocyte responses
    ZHI-LIANG DUAN, ZHI-BIN WANG, JIANG-LONG GUO, WEN-QUAN LIU, JUN HU, JING LI, SI-NA WANG, QIANG LI, JIN-SHENG WEN
    International Journal of Oncology.2013; 42(4): 1482.     CrossRef
  • 10,947 View
  • 93 Download
  • Crossref
Brief Communications
Maintained Seroprevalence of Toxoplasmosis among the Residents of Jeju Island, Korea
Sung-Jong Hong, Chom-Kyu Chong, Kyutaeg Lee, Tong-Soo Kim, Yeon-Pyo Hong, Hye-Jin Ahn, Hak-Yong Kim, A-Ra Ko, Yong-Joo Kim, Ho-Woo Nam
Korean J Parasitol 2011;49(3):309-311.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.309

Seroepidemiological status of toxoplasmosis among the residents of Jeju island was surveyed and evaluated by ELISA with crude extract of Toxoplasma gondii. The sera of 2,348 residents (male 1,157 and female 1,191) were collected and checked for the IgG antibody titers, which showed 13.2% positive rate (309 sera). The positive rates were increasing gradually according to the age from 4.3% in teenage to 20.6% in seventies. The positive rates were significantly different between the sex by 16.2% for male and 10.2% for female (P<0.05). This positive rate of toxoplasmosis in Jeju island residents is regarded relatively higher than any other regions of Korea. And the high positive rate may be maintained continuously among Jeju island residents without any clear reasons until now but due to some parts peculiar socio-cultural tradition of Jeju island. Therefore, it is necessary to study further the epidemiology of toxoplasmosis of Jeju island.

Citations

Citations to this article as recorded by  Crossref logo
  • Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea
    Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(1): 9.     CrossRef
  • Clusters of Toxoplasmosis in Ganghwa-gun, Cheorwon-gun, and Goseong-gun, Korea
    Jihye Yu, Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(3): 251.     CrossRef
  • Cross-sectional survey for Toxoplasma gondii infection in humans in Fernando de Noronha island, Brazil
    Maria da Conceição Carvalho, Müller Ribeiro-Andrade, Renata Pimentel Bandeira de Melo, Dandara Matias Guedes, José Wilton Pinheiro Junior, Erika Fernanda Torres Samico Fernandes Cavalcanti, Fernando Jorge Rodrigues Magalhães, Rinaldo Aparecido Mota
    Revista Brasileira de Parasitologia Veterinária.2021;[Epub]     CrossRef
  • Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea
    Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho
    The Korean Journal of Parasitology.2020; 58(3): 257.     CrossRef
  • Clusters of Toxoplasmosis in Gyodong-Myeon and Samsan-Myeon, Ganghwa-Gun, Korea
    Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2020; 58(5): 493.     CrossRef
  • Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
    Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong
    The Korean Journal of Parasitology.2018; 56(5): 437.     CrossRef
  • Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up
    Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(3): 247.     CrossRef
  • Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea
    Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(4): 385.     CrossRef

  • Koki Yakushiji
    JOURNAL OF WATER AND ENVIRONMENTAL ISSUES.2015; 28(1): 82.     CrossRef
  • Mesial Temporal Lobe Epilepsy in Congenital Toxoplasmosis: A Case Report
    Woo Kyo Jeong, Byung-Euk Joo, Ji-hye Seo, Jun Kyu Mun, Juhyeon Kim, Dae-Won Seo
    Journal of Epilepsy Research.2015; 5(1): 25.     CrossRef
  • Toxoplasma gondii Infection: Seroprevalence and Associated Risk Factors among Primary School Children in the Capital Area of the Republic of the Marshall Islands
    Chung-Jung Fu, Ting-Wu Chuang, Huei-Shan Lin, Chih-Hsiung Wu, Yung-Ching Liu, Mailynn K. Langinlur, Min-Yun Lu, Wesley Wei-Wen Hsiao, Chia-Kwung Fan
    Japanese Journal of Infectious Diseases.2014; 67(5): 405.     CrossRef
  • A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
    Kyoung Ju Song, Zhaoshou Yang, Chom-Kyu Chong, Jin-Soo Kim, Kyung Chan Lee, Tong-Soo Kim, Ho-Woo Nam
    The Korean Journal of Parasitology.2013; 51(5): 503.     CrossRef
  • Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
    Hyemi Lim, Sang-Eun Lee, Bong-Kwang Jung, Min-Ki Kim, Mi Youn Lee, Ho-Woo Nam, Jong-Gyun Shin, Cheong-Ha Yun, Han-Ik Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2012; 50(4): 287.     CrossRef
  • A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea
    Zhaoshou Yang, Pyo Yun Cho, Seong Kyu Ahn, Hye-Jin Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam
    The Korean Journal of Parasitology.2012; 50(3): 191.     CrossRef
  • Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea
    Hye-Jin Ahn, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam
    The Korean Journal of Parasitology.2012; 50(3): 225.     CrossRef
  • Acute Toxoplasmosis Mimicking Melanoma Metastases: Review of Conditions Causing False-Positive Results on 18F-FDG PET/CT
    K. Ivanova, K. Glatz, A. Zippelius, G. Nicolas, P. Itin
    Dermatology.2012; 225(4): 349.     CrossRef
  • 8,545 View
  • 82 Download
  • Crossref
Resistance to Toxoplasma gondii Infection in Mice Treated with Silk Protein by Enhanced Immune Responses
Joung-Ho Moon, Kyoung-Ho Pyo, Bong-Kwang Jung, Hyang Sook Chun, Jong-Yil Chai, Eun-Hee Shin
Korean J Parasitol 2011;49(3):303-308.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.303

This study investigated whether elevated host immune capacity can inhibit T. gondii infection. For this purpose, we used silk protein extracted from Bombyx mori cocoons as a natural supplement to augment immune capacity. After silk protein administration to BALB/c mice for 6 weeks, ratios of T lymphocytes (CD4+ and CD8+ T-cells) and splenocyte proliferative capacities in response to Con A or T. gondii lysate antigen (TLA) were increased. Of various cytokines, which regulate immune systems, Th1 cytokines, such as IFN-γ, IL-2, and IL-12, were obviously increased in splenocyte primary cell cultures. Furthermore, the survival of T. gondii (RH strain)-infected mice increased from 2 days to 5 or more days. In a state of immunosuppression induced by methylprednisolone acetate, silk protein-administered mice were resistant to reduction in T-lymphocyte (CD4+ and CD8+ T-cells) numbers and the splenocyte proliferative capacity induced by Con A or TLA with a statistical significance. Taken together, our results suggest that silk protein augments immune capacity in mice and the increased cellular immunity by silk protein administration increases host protection against acute T. gondii infection.

Citations

Citations to this article as recorded by  Crossref logo
  • Oral Toxicity and Hypotensive Influence of Sericin-Derived Oligopeptides (SDOs) from Yellow Silk Cocoons of Bombyx mori in Rodent Studies
    Chainarong Tocharus, Virakboth Prum, Manote Sutheerawattananonda
    Foods.2024; 13(21): 3505.     CrossRef
  • Low dose administration of mature silkworm powder induces gastric mucosal defense factors in ethanol-induced gastric injury rat model
    Da-Young Lee, Moon-Young Song, Kyung-Sook Hong, Sun-Mi Yun, Young-Min Han, Eun-Hee Kim
    Food Science and Biotechnology.2023; 32(11): 1551.     CrossRef
  • Dietary administration of silk microparticles improves the epidermal and dermal regeneration after a skin wounding in gilthead seabream (Sparus aurata L.)
    Nora Albaladejo-Riad, Cristóbal Espinosa Ruiz, M. Ángeles Esteban
    Fish & Shellfish Immunology.2022; 124: 92.     CrossRef
  • The effect of silk peptide on immune system, A randomized, double-blind, placebo-controlled clinical trial
    Jin-Taek Hwang, Jung Min Cho, In Hye Jeong, Jeong-yong Lee, Ki-Chan Ha, Hyang-Im Baek, Hye Jeong Yang, Min Jung Kim, Jong Ho Lee
    Journal of Functional Foods.2019; 55: 275.     CrossRef
  • Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes
    Sun-Hee Jang, Mi-Sun Oh, Hyang-Im Baek, Ki-Chan Ha, Jeong-Yong Lee, Yong-Suk Jang
    Pharmaceutical Biology.2019; 57(1): 369.     CrossRef
  • Oral Administration of Silk Peptide Enhances the Maturation and Cytolytic Activity of Natural Killer Cells
    Sun-Hee Jang, Mi-Sun Oh, Hyang-Im Baek, Ki-Chan Ha, Jeong-Yong Lee, Yong-Suk Jang
    Immune Network.2018;[Epub]     CrossRef
  • Biomaterials-Based Vaccination Strategies for the Induction of CD8+T Cell Responses
    Charles B. Chesson, Shaunte Ekpo-Otu, Janice J. Endsley, Jai S. Rudra
    ACS Biomaterials Science & Engineering.2017; 3(2): 126.     CrossRef
  • STAT6 Expression and IL-13 Production in Association with Goblet Cell Hyperplasia and Worm Expulsion of Gymnophalloides seoi from C57BL/6 Mice
    Jin-Joo Lee, Donghee Kim, Kyoung-Ho Pyo, Min-Ki Kim, Hyo-Jin Kim, Jong-Yil Chai, Eun-Hee Shin
    The Korean Journal of Parasitology.2013; 51(5): 589.     CrossRef
  • 9,676 View
  • 88 Download
  • Crossref
First First Prev Prev
Page of 2
Next Next Last Last
PHD : Parasites, Hosts and Diseases