Parasites, Hosts and Diseases

"protein"

Original Articles

Functional characterization of glucose transporter 4 involved in glucose uptake in Clonorchis sinensis: Hojong Jun, Ernest Mazigo, Wang-Jong Lee, Yun-Kyu Park, Jin-Hee Han, Seok Ho Cha; Parasites Hosts Dis 2024;62(4):450-460.
Published online November 22, 2024; DOI: https://doi.org/10.3347/PHD.24051

Clonorchis sinensis, which causes clonorchiasis, is prevalent in East Asian countries and poses notable health risks, including bile duct complications. Although praziquantel is the primary treatment for the disease, the emerging resistance among trematodes highlights the need for alternative strategies. Understanding the nutrient uptake mechanisms in trematodes, including C. sinensis, is crucial for developing future effective treatments. This study aimed to characterize the function of C. sinensis glucose transporter 4 (CsGTP4) and determine its role in nutrient uptake employing synthesized cDNA of adult C. sinensis worms. The functional characterization of CsGTP4 involved injecting its cRNA into Xenopus laevis oocytes and analyzing the deoxy-D-glucose uptake levels. The results demonstrated that deoxy-D-glucose uptake depended on the deoxy-D-glucose incubation and CsGTP4 expression time, but not sodium-dependent. The concentration-dependent uptake followed the Michaelis–Menten equation, with a Km value of 2.7 mM and a Vmax value of 476 pmol/oocyte/h based on the Lineweaver–Burk analysis. No uptake of radiolabeled α-ketoglutarate, p-aminohippurate, taurocholate, arginine, or carnitine was observed. The uptake of deoxy-D-glucose by CsGTP4 was significantly inhibited by unlabeled glucose and galactose in a concentration-dependent manner. It was significantly inhibited under strongly acidic and basic conditions. These insights into the glucose uptake kinetics and pH dependency of CsGTP4 provide a deeper understanding of nutrient acquisition in trematodes. This study contributes to the development of novel antiparasitic agents, addressing a considerable socioeconomic challenge in affected regions.

Citations

Citations to this article as recorded by

Clonorchis sinensis dopamine transporter (CsDAT) facilitates dopamine uptake
Wang-Jong Lee, Sung-Jun Kim, Woon Kyu Lee, Jin-Hee Han, Seok Ho Cha
Parasites, Hosts and Diseases.2025; 63(3): 215. CrossRef

2,405 View
83 Download
1 Web of Science
Crossref

Identification of the protease inhibitory domain of Trichinella spiralis novel cystatin (TsCstN): Thassanee Yuthithum, Orawan Phuphisut, Onrapak Reamtong, Nathamon Kosoltanapiwat, Salisa Chaimon, Porntida Kobpornchai, Charin Thawornkuno, Preeyarat Malaithong, Orathai Sawatdichaikul, Poom Adisakwattana; Parasites Hosts Dis 2024;62(3):330-341.
Published online August 26, 2024; DOI: https://doi.org/10.3347/PHD.24026

The Trichinella spiralis novel cystatin (TsCstN) inhibits cathepsin L (CatL) activity and inflammation of macrophages during lipopolysaccharide (LPS) induction. To identify the protease inhibitory region, this study applied an in silico modeling approach to simulate truncation sites of TsCstN (Ts01), which created four truncated forms, including TsCstN^∆1-39 (Ts02), TsCstN^∆1-71 (Ts03), TsCstN^{∆1-20, ∆73-117} (Ts04), and TsCstN^{∆1-20, ∆42-117} (Ts05). The superimposition of these truncates modeled with AlphaFold Colab indicated that their structures were more akin to Ts01 than those modeled with I-TASSER. Moreover, Ts04 exhibited the closest resemblance to the structure of Ts01. The recombinant Ts01 (rTs01) and truncated proteins (rTs02, rTs03, and rTs04) were successfully expressed in a prokaryotic expression system while Ts05 was synthesized, with sizes of approximately 14, 12, 8, 10, and 2.5 kDa, respectively. When determining the inhibition of CatL activity, both rTs01 and rTs04 effectively reduced CatL activity in vitro. Thus, the combination of the α1 and L1 regions may be sufficient to inhibit CatL. This study provides comprehensive insights into TsCstN, particularly regarding its protein function and inhibitory domains against CatL.

Citations

Citations to this article as recorded by

Systems biology of Haemonchus contortus – Advancing biotechnology for parasitic nematode control
Yuanting Zheng, Neil D. Young, Tao Wang, Bill C.H. Chang, Jiangning Song, Robin B. Gasser
Biotechnology Advances.2025; 81: 108567. CrossRef
Therapeutic potentials of Trichinella spiralis in immune disorders: From allergy to autoimmunity
Minkyoung Cho, Hak Sun Yu
Parasites, Hosts and Diseases.2025; 63(2): 123. CrossRef

2,597 View
71 Download
2 Web of Science
Crossref

Mini Review

Recent progress in vaccine development targeting pre-clinical human toxoplasmosis: Ki-Back Chu, Fu-Shi Quan; Parasites Hosts Dis 2023;61(3):231-239.
Published online August 21, 2023; DOI: https://doi.org/10.3347/PHD.22097

Abstract
PDF

Toxoplasma gondii is an intracellular parasitic organism affecting all warm-blooded vertebrates. Due to the unavailability of commercialized human T. gondii vaccine, many studies have been reported investigating the protective efficacy of pre-clinical T. gondii vaccines expressing diverse antigens. Careful antigen selection and implementing multifarious immunization strategies could enhance protection against toxoplasmosis in animal models. Although none of the available vaccines could remove the tissue-dwelling parasites from the host organism, findings from these pre-clinical toxoplasmosis vaccine studies highlighted their developmental potential and provided insights into rational vaccine design. We herein explored the progress of T. gondii vaccine development using DNA, protein subunit, and virus-like particle vaccine platforms. Specifically, we summarized the findings from the pre-clinical toxoplasmosis vaccine studies involving T. gondii challenge infection in mice published in the past 5 years.

Citations

Citations to this article as recorded by

PLGA nanoparticles as an efficient carrier in Toxoplasma GAP45: a more effective vaccine against acute toxoplasmosis than traditional ones
Pan Zhou, YanLi Yu, WeiYu Qi, XiaoJuan Wang, YouLi Yu, JianDong Wang, Li Zhang, ZhengQing Yu, TingLi Liu
Frontiers in Immunology.2025;[Epub] CrossRef
Influenza virus-like particles presenting Toxoplasma gondii dense granule protein 7 protect mice from lethal ME49 challenge
Jie Mao, Hae-Ji Kang, Su-In Heo, Fu-Shi Quan
Nanomedicine.2025; 20(18): 2309. CrossRef
Recombinant TgDDX3X DEAD-box protein confers partial protection in murine models of acute and chronic toxoplasmosis
Shuai Wang, Jinghui Wang, Youke Fan, Haina Zhang, Junru Wu, Tingting Ying, Hangbin Ma, Qiangqiang Wang, Longkang Wang, Yuanfeng Wang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang, Zhenke Yang
Acta Tropica.2025; 270: 107780. CrossRef
Vaccinia virus expressing MIC8 and AMA1 provides protection against Toxoplasma gondii ME49 infection
Hae-Ji Kang, Yan Jin, Zhao-Shou Yang, Md Atique Ahmed, Fu-Shi Quan
Parasites, Hosts and Diseases.2025; 63(4): 340. CrossRef
Evaluation of protective efficacy of recombinant Toxoplasma gondii DDX39 protein vaccine against acute and chronic T. gondii infection in mice
Jinghui Wang, Yuanfeng Wang, Haina Zhang, Hangbin Ma, Qiangqiang Wang, Longkang Wang, Youke Fan, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang, Shuai Wang, Zhenke Yang
Acta Tropica.2024; 260: 107442. CrossRef

8,208 View
139 Download
4 Web of Science
Crossref

Original Articles

Genetic diversity of Plasmodium falciparum erythrocyte membrane protein 1 in field isolates from central Myanmar: Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Zin Moon, Dong-Il Chung, Yeonchul Hong, Moe Kyaw Myint, Haung Naw, Byoung-Kuk Na, Youn-Kyoung Goo; Parasites Hosts Dis 2023;61(1):24-32.
Published online February 22, 2023; DOI: https://doi.org/10.3347/PHD.22165

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), encoded by the polymorphic var multigene family, is a highly polymorphic antigen that plays a crucial role in the pathology of malaria. The contribution of the genetic diversity of var toward the immune escape of P. falciparum has not yet been fully elucidated. This study aimed to characterize the diversity of var repertoires by screening P. falciparum Duffy-binding-like α domain (PfDBLα) among field isolates from central Myanmar. Genetic analysis revealed that the D-H segments of var in Myanmar populations have an extensive polymorphic repertoire, with high numbers of unique sequence types in each individual. However, var genes from the global population, including Myanmar, shared close genetic lineages regardless of their geographic origins, indicating that they have not undergone rapid evolutionary changes.

Citations

Citations to this article as recorded by

Exploring the Potential of miRNA-92a-3p as Lead for Sequence-Based Therapies for Malaria
Sowmya R. Prabhu, Sayandrila Paul, Shashikiran Umakanth, Manjunath Hande, Abdul Vahab Saadi, Himanshu Gupta, Kapaettu Satyamoorthy
Acta Parasitologica.2025;[Epub] CrossRef
Genetic diversity of Plasmodium falciparum erythrocyte membrane protein 1 in field isolates: Correspondence
Amnuay Kleebayoon, Viroj Wiwanitkit
Parasites, Hosts and Diseases.2023; 61(3): 338. CrossRef

5,304 View
183 Download
3 Web of Science
Crossref

Genetic Diversity and Clustering of the Rhoptry Associated Protein-1 of Plasmodium knowlesi from Peninsular Malaysia and Malaysian Borneo: Ummi Wahidah Azlan, Yee Ling Lau, Mun Yik Fong; Korean J Parasitol 2022;60(6):393-400.
Published online December 22, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.6.393

Human infection with simian malaria Plasmodium knowlesi is a cause for concern in Southeast Asian countries, especially in Malaysia. A previous study on Peninsular Malaysia P. knowlesi rhoptry associated protein-1 (PkRAP1) gene has discovered the existence of dimorphism. In this study, genetic analysis of PkRAP1 in a larger number of P. knowlesi samples from Malaysian Borneo was conducted. The PkRAP1 of these P. knowlesi isolates was PCR-amplified and sequenced. The newly obtained PkRAP1 gene sequences (n = 34) were combined with those from the previous study (n = 26) and analysed for polymorphism and natural selection. Sequence analysis revealed a higher genetic diversity of PkRAP1 compared to the previous study. Exon II of the gene had higher diversity (π = 0.0172) than exon I (π = 0.0128). The diversity of the total coding region (π = 0.0167) was much higher than those of RAP1 orthologues such as PfRAP-1 (π = 0.0041) and PvRAP1 (π = 0.00088). Z-test results indicated that the gene was under purifying selection. Phylogenetic tree and haplotype network showed distinct clustering of Peninsular Malaysia and Malaysian Borneo PkRAP1 haplotypes. This geographical-based clustering of PkRAP1 haplotypes provides further evidence of the dimorphism of the gene and possible existence of 2 distinct P. knowlesi lineages in Malaysia.

Citations

Citations to this article as recorded by

Subtype–host patterns and genetic differentiation of Blastocystis sp. in the Philippines
Davin Edric V. Adao, Windell L. Rivera
Heliyon.2024; 10(7): e29019. CrossRef
Vector-borne diseases on Borneo island: a scoping review
Song-Quan Ong, Ag Shazmeer Ag Safree, Nur Badriah Asmail Ismai
Malaria Journal.2024;[Epub] CrossRef
Inter-Population Genetic Diversity and Clustering of Merozoite Surface Protein-1 (pkmsp-1) of Plasmodium knowlesi Isolates from Malaysia and Thailand
Naqib Rafieqin Noordin, Yee Ling Lau, Fei Wen Cheong, Mun Yik Fong
Tropical Medicine and Infectious Disease.2023; 8(5): 285. CrossRef

3,070 View
124 Download
3 Web of Science
Crossref

Brief Communication

Specific Detection of Acanthamoeba species using Polyclonal Peptide Antibody Targeting the Periplasmic Binding Protein of A. castellanii: Min-Jeong Kim, Fu-Shi Quan, Hyun-Hee Kong, Jong-Hyun Kim, Eun-Kyung Moon; Korean J Parasitol 2022;60(2):143-147.
Published online April 20, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.2.143

Abstract
PDF

Acanthamoeba keratitis (AK) is a rare ocular disease, but it is a painful and sight-threatening infectious disease. Early diagnosis and adequate treatment are necessary to prevent serious complications. While AK is frequently diagnosis via several PCR assays or Acanthamoeba-specific antibodies, a more specific and effective diagnostic method is required. This study described the production of a polyclonal peptide antibody against the periplasmic binding protein (PBP) of A. castellanii and investigated its diagnostic potential. Western blot analysis showed that the PBP antibody specifically reacted with the cell lysates of A. castellanii. However, the PBP antibody did not interact with human corneal epithelial (HCE) cells and the other 3 major causative agents of keratitis. Immunocytochemistry (ICC) results revealed the specific detection of A. castellanii trophozoites and cysts by PBP antibodies when A. castellanii were co-cultured with HCE cells. PBP antibody specificity was further confirmed by co-culture of A. castellanii trophozoites with F. solani, S. aureus, and P. aeruginosa via ICC. The PBP antibody specifically reacted with the trophozoites and cysts of A. polyphaga, A. hatchetti, A. culbertsoni, A. royreba, and A. healyi, thus demonstrated its genus-specific nature. These results showed that the PBP polyclonal peptide antibody of A. castellanii could specifically detect several species of Acanthamoeba, contributing to the development of an effective antibody-based AK diagnostics.

Citations

Citations to this article as recorded by

Evaluation of the potential for diagnosis of fungal keratitis using a Fusarium-specific antibody
Hye-Jeong Jo, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
Scientific Reports.2025;[Epub] CrossRef
Detection of Fusarium solani using cutinase antibody and its application in diagnosing fungal keratitis in an animal model
Hye-Jeong Jo, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon, Olaf Kniemeyer
PLOS One.2025; 20(8): e0330455. CrossRef
A chorismate mutase-targeted, core-shell nanoassembly-activated SERS immunoassay platform for rapid non-invasive detection of Acanthamoeba infection
Hyerin Lee, Min-Jeong Kim, Junkyu Chung, Wansun Kim, Hye-Jeong Jo, Tae Gi Kim, Jae-Ho Shin, Gi-Ja Lee, Fu-Shi Quan, Hyun-Hee Kong, Sang Woong Moon, Eun-Kyung Moon, Samjin Choi
Nano Today.2024; 59: 102506. CrossRef
Evaluating the Diagnostic Potential of Chorismate Mutase Poly-Clonal Peptide Antibody for the Acanthamoeba Keratitis in an Animal Model
Min-Jeong Kim, Hye-Jeong Jo, Hae-Jin Sohn, Ho-Joon Shin, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
Pathogens.2023; 12(4): 526. CrossRef
Detection of Acanthamoeba from Acanthamoeba Keratitis Mouse Model Using Acanthamoeba-Specific Antibodies
Min-Jeong Kim, A-Jeong Ham, A-Young Park, Hae-Jin Sohn, Ho-Joon Shin, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
Microorganisms.2022; 10(9): 1711. CrossRef

3,770 View
196 Download
5 Web of Science
Crossref

Original Articles

Characterization of a Peptide Antibody Specific to the Adenylyl Cyclase-Associated Protein of Acanthamoeba castellanii: Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon; Korean J Parasitol 2022;60(1):7-14.
Published online February 23, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.1.7

Abstract
PDF

Acanthamoeba keratitis (AK) is a rare infectious disease and accurate diagnosis has remained arduous as clinical manifestations of AK were similar to keratitis of viral, bacterial, or fungal origins. In this study, we described the production of a polyclonal peptide antibody against the adenylyl cyclase-associated protein (ACAP) of A. castellanii, and evaluated its differential diagnostic potential. Enzyme-linked immunosorbent assay revealed high titers of A. castellanii-specific IgG and IgA antibodies being present in low dilutions of immunized rabbit serum. Western blot analysis revealed that the ACAP antibody specifically interacted with A. castellanii, while not interacting with human corneal epithelial (HCE) cells and other causes of keratitis such as Fusarium solani, Pseudomonas aeruginosa, and Staphylococcus aureus. Immunocytochemistry (ICC) results confirmed the specific detection of trophozoites and cysts of A. castellanii co-cultured with HCE cells. The ACAP antibody also specifically interacted with the trophozoites and cysts of 5 other Acanthamoeba species. These results indicate that the ACAP antibody of A. castellanii can specifically detect multiple AK-causing members belonging to the genus Acanthamoeba and may be useful for differentially diagnosing Acanthamoeba infections.

Citations

Citations to this article as recorded by

The pathogenesis, risk factors, diagnosis and treatment of Acanthamoeba keratitis
Mingliang Bao, Hai Bao, Shuqing Wang, Hongyan Zhou
Frontiers in Medicine.2025;[Epub] CrossRef
A chorismate mutase-targeted, core-shell nanoassembly-activated SERS immunoassay platform for rapid non-invasive detection of Acanthamoeba infection
Hyerin Lee, Min-Jeong Kim, Junkyu Chung, Wansun Kim, Hye-Jeong Jo, Tae Gi Kim, Jae-Ho Shin, Gi-Ja Lee, Fu-Shi Quan, Hyun-Hee Kong, Sang Woong Moon, Eun-Kyung Moon, Samjin Choi
Nano Today.2024; 59: 102506. CrossRef
Evaluating the Diagnostic Potential of Chorismate Mutase Poly-Clonal Peptide Antibody for the Acanthamoeba Keratitis in an Animal Model
Min-Jeong Kim, Hye-Jeong Jo, Hae-Jin Sohn, Ho-Joon Shin, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
Pathogens.2023; 12(4): 526. CrossRef
New Frontiers in Acanthamoeba Keratitis Diagnosis and Management
Omar Shareef, Sana Shareef, Hajirah N. Saeed
Biology.2023; 12(12): 1489. CrossRef
Detection of Acanthamoeba from Acanthamoeba Keratitis Mouse Model Using Acanthamoeba-Specific Antibodies
Min-Jeong Kim, A-Jeong Ham, A-Young Park, Hae-Jin Sohn, Ho-Joon Shin, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
Microorganisms.2022; 10(9): 1711. CrossRef

4,767 View
179 Download
5 Web of Science
Crossref

Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein: So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2022;60(1):1-6.
Published online February 23, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.1.1

Abstract
PDF

The encystation of Acanthamoeba leads to the development of metabolically inactive and dormant cysts from vegetative trophozoites under unfavorable conditions. These cysts are highly resistant to anti-Acanthamoeba drugs and biocides. Therefore, the inhibition of encystation would be more effective in treating Acanthamoeba infection. In our previous study, a sirtuin family protein—Acanthamoeba silent-information regulator 2-like protein (AcSir2)—was identified, and its expression was discovered to be critical for Acanthamoeba castellanii proliferation and encystation. In this study, to develop Acanthamoeba sirtuin inhibitors, we examine the effects of sirtinol, a sirtuin inhibitor, on trophozoite growth and encystation. Sirtinol inhibited A. castellanii trophozoites proliferation (IC50=61.24 μM). The encystation rate of cells treated with sirtinol significantly decreased to 39.8% (200 μM sirtinol) after 24 hr of incubation compared to controls. In AcSir2-overexpressing cells, the transcriptional level of cyst-specific cysteine protease (CSCP), an Acanthamoeba cysteine protease involved in the encysting process, was 11.6- and 88.6-fold higher at 48 and 72 hr after induction of encystation compared to control. However, sirtinol suppresses CSCP transcription, resulting that the undegraded organelles and large molecules remained in sirtinol-treated cells during encystation. These results indicated that sirtinol sufficiently inhibited trophozoite proliferation and encystation, and can be used to treat Acanthamoeba infections.

Citations

Citations to this article as recorded by

Pterostilbene: A natural neuroprotective stilbene with anti-Alzheimer's disease properties
Songlan Gao, Honglei Zhang, Na Li, Lijuan Zhang, Zhe Zhu, Changlu Xu
Journal of Pharmaceutical Analysis.2025; 15(4): 101043. CrossRef
Alzheimer’s Disease: A Review of Molecular Mechanisms and Therapeutic Implications by Targeting Sirtuins, Caspases, and GSK-3
Kalpana Pandya, Krishnashish Roul, Avanish Tripathi, Sateesh Belemkar, Anshuman Sinha, Meryem Erol, Devendra Kumar
ACS Chemical Neuroscience.2025; 16(12): 2178. CrossRef
Human Conjunctival Transcriptome in Acanthamoeba Keratitis: An Exploratory Study
Gerami D. Seitzman, Jeremy D. Keenan, Thomas M. Lietman, Kevin Ruder, Lina Zhong, Cindi Chen, YuHeng Liu, Danny Yu, Thomas Abraham, Armin Hinterwirth, Thuy Doan
Cornea.2024; 43(10): 1272. CrossRef
Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells
Abdullah Alhazmi, Laura E. Sidney, Andy Hopkinson, Hany M. Elsheikha
Acta Tropica.2024; 257: 107288. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef

4,722 View
252 Download
5 Web of Science
Crossref

Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen: Min-Ju Kim, Jie Mao, Hae-Ji Kang, Ki-Back Chu, Fu-Shi Quan; Korean J Parasitol 2021;59(6):565-572.
Published online December 22, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.6.565

Toxoplasma gondii ME49 infections are typically diagnosed by serological tests. However, serological diagnosis of RH strain-induced toxoplasmosis remains unknown. In order to develop seradiagnosis of above 2 kinds of infections, we generated recombinant virus-like particles (VLPs) displaying the T. gondii rhoptry protein 4 (ROP4) and evaluated their potential in T. gondii ME49 or RH strain infection diagnostics. Mice were orally infected with either the tachyzoites of T. gondii (RH) or cysts of T. gondii (ME49) at various dosages, and sera were collected at regular intervals. ELISA-based serological tests were performed to assess IgG, IgM, and IgA antibody responses against ROP4 VLP antigen and tissue lysate antigen (TLA). Compared to TLA, IgG, IgM, and IgA levels to ROP4 VLP antigen were significantly higher in the sera of T. gondii RH-infected mice 1 and 2 week post-infection (PI). T. gondii-specific IgG antibody was detected at 1, 2, 4, and 8 week PI in the T. gondii ME49-infected mice with infection dose-dependent manner. These results indicated that the ROP4 VLP antigen was highly sensitive antigens detecting T. gondii RH and ME49 antibodies at an early stage.

Citations

Citations to this article as recorded by

IgM Antibody Detection as a Diagnostic Marker for Acute Toxoplasmosis: Current Status of Studies and Main Limitations
Karolina Sołowińska, Lucyna Holec-Gąsior
Antibodies.2025; 14(2): 44. CrossRef
Could metformin modulate the outcome of chronic murine toxoplasmosis?
Maha Mohamed Gomaa, Samar Nabil El Achy, Nehal Nassef Hezema
Acta Tropica.2024; 258: 107339. CrossRef
Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection
Min-ju Kim, Soeun J. Park, Hyunwoo Park
European Journal of Medical Research.2024;[Epub] CrossRef
Recombinant AMA1 Virus-like Particle Antigen for Serodiagnosis of Toxoplasma gondii Infection
Min-Ju Kim, Ki-Back Chu, Jie Mao, Hae-Ji Kang, Gi-Deok Eom, Keon-Woong Yoon, Su-Hwa Lee, Eun-Kyung Moon, Young-Ha Lee, Fu-Shi Quan
Biomedicines.2022; 10(11): 2812. CrossRef

4,641 View
95 Download
4 Web of Science
Crossref

Brief Communications

Seroprevalence of Plasmodium vivax Circumsporozoite Protein Antibody in High-Risk Malaria Areas in Korea: Jinyoung Lee, Kyoung Jin, Seong Kyu Ahn, Sung-Keun Lee, Hyung Wook Kwon, Byoung-Kuk Na, Tong-Soo Kim; Korean J Parasitol 2021;59(4):415-419.
Published online August 18, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.4.415

The circumsporozoite protein (CSP) of Plasmodium spp. is a diagnostic antigen and useful biomarker for monitoring short-term/seasonal changes to malaria transmission. Using P. vivax CSP antibody ELISA, epidemiological characteristics were analyzed in the residents of Ganghwa, Cheorwon, Paju, and Goseong from 2017 to 2018. In Ganghwa and Cheorwon, 1.6% and 1.2% of residents, respectively, were PvCSP-antibody-positive in 2018, which indicates a decrease of 0.4% in the positive rate compared to 2017. The annual parasite incidence (API) in Ganghwa and Cheorwon was 24.9 and 10.5 in 2017 and 20.3 and 10.7 in 2018, respectively. Although the changes were not significant, the API in Ganghwa decreased slightly by 4.5 in 2018 compared to the previous year. In Paju and Goseong, 3.9% and 2.0% of residents were positive for the PvCSP antibody. The API in Paju was 13.1 in 2017 and 16.0 in 2018, although no malaria patients were reported for the 2 years. Therefore, the results suggest that PvCSP is a useful antigen for confirming initial malaria infection. Additionally, considering that the antibody is relatively transient, it can be employed for sero-epidemiological studies to determine the extent of malaria transmission in the current year.

Citations

Citations to this article as recorded by

Using Serological Markers for the Surveillance of Plasmodium vivax Malaria: A Scoping Review
Lejla Kartal, Ivo Mueller, Rhea J. Longley
Pathogens.2023; 12(6): 791. CrossRef
Mosquirix™ RTS, S/AS01 Vaccine Development, Immunogenicity, and Efficacy
Aroosa Younis Nadeem, Adeeb Shehzad, Salman Ul Islam, Ebtesam A. Al-Suhaimi, Young Sup Lee
Vaccines.2022; 10(5): 713. CrossRef
Serological responses to a soluble recombinant circumsporozoite protein-VK210 of Plasmodium vivax (rPvCSP-VK210) among Iranian malaria patients
Mehdi Nateghpour, Soudabeh Etemadi, Afsaneh Motevalli Haghi, Hamid Eslami, Mehdi Mohebali, Leila Farivar
European Journal of Medical Research.2021;[Epub] CrossRef

4,462 View
114 Download
3 Web of Science
Crossref

Molecular Identification of Cryptosporidium viatorum Infection in a Patient Suffering from Unusual Cryptosporidiosis in West Bengal, India: Sanjib Kumar Sardar, Ajanta Ghosal, Yumiko Saito-Nakano, Shanta Dutta, Tomoyoshi Nozaki, Sandipan Ganguly; Korean J Parasitol 2021;59(4):409-413.
Published online August 18, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.4.409

Abstract
PDF

In this study, we have collected and screened a total of 268 stool samples from diarrheal patients admitted to an Infectious disease hospital in Kolkata for the presence of Cryptosporidium spp. The initial diagnosis was carried out by microscopy followed by genus specific polymerase chain reaction assays based on 70 kDa heat shock proteins (HSP70). DNA sequencing of the amplified locus has been employed for determination of genetic diversity of the local isolates. Out of 268 collected samples, 12 (4.48%) were positive for Cryptosporidium spp. Sequences analysis of 70 kDa heat shock proteins locus in 12 Cryptosporidium local isolates revealed that 2.24% and 1.86% of samples were showing 99% to 100% identity with C. parvum and C. hominis. Along with the other 2 major species one recently described globally distributed pathogenic species Cryptosporidium viatorum has been identified. The HSP70 locus sequence of the isolate showed 100% similarity with a previously described isolate of C. viatorum (Accession No. JX978274.1, JX978273.1, and JN846706.1) present in GenBank.

Citations

Citations to this article as recorded by

Prevalence and genotype analysis of Cryptosporidium spp. in nine species of wild rodents in China
Zhen-Qiu Gao, Hai-Tao Wang, Jing-Hao Li, Yi-Xuan Song, Qing-Yu Hou, Si-Yuan Qin, He Ma, Quan Zhao, Ya Qin
Parasite.2025; 32: 19. CrossRef
Cryptosporidium and cryptosporidiosis: An update of Asian perspectives in humans, water and food, 2015–2025
Shahira Abdelaziz Ali Ahmed, Sonia Boughattas, Mohammad Reza Mahmoudi, Huma Khan, Simuzar Mamedova, Ardra Namboodiri, Frederick R. Masangkay, Panagiotis Karanis
Current Research in Parasitology & Vector-Borne Diseases.2025; 8: 100311. CrossRef
Critters and contamination: Zoonotic protozoans in urban rodents and water quality
Siobhon Egan, Amanda D. Barbosa, Yaoyu Feng, Lihua Xiao, Una Ryan
Water Research.2024; 251: 121165. CrossRef
An Update on Zoonotic Cryptosporidium Species and Genotypes in Humans
Una Ryan, Alireza Zahedi, Yaoyu Feng, Lihua Xiao
Animals.2021; 11(11): 3307. CrossRef

4,445 View
97 Download
5 Web of Science
Crossref

Evaluation of Rhophilin Associated Tail Protein (ROPN1L) in the Human Liver Fluke Opisthorchis viverrini for Diagnostic Approach: Amornrat Geadkaew-Krenc, Rudi Grams, Wansika Phadungsil, Wanlapa Chaibangyang, Nanthawat Kosa, Poom Adisakwattana, Paron Dekumyoy; Korean J Parasitol 2020;58(4):475-479.
Published online August 25, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.4.475

Tegumental and excretory-secretory proteins are reported as diagnostic antigens for human opisthorchiasis. Rhophilin associated tail protein1-like (OvROPN1L) protein of Opisthorchis viverrini sperm tail showed potential as a diagnostic antigen. The OvROPN1L recombinant fragments were assayed for diagnostic antigenicity for human opisthorchiasis using indirect ELISA. The strongest antigenic region was a N-terminus peptide of M1 - P56. One synthetic peptide (P1, L3-Q13) of this region showed the highest antigenicity to opisthorchiasis. Sera from other parasitic infections including Strongyloides stercoralis, hookworm, Taenia spp, minute intestinal flukes, Paragonimus spp showed lower reactivity to P1. Peptide P1 is located in the disordered N-terminus of ROPN1L supporting its suitability as linear epitope. In the Platyhelminthes the N-terminal sequence of ROPN1L is diverging with taxonomic distance further suggesting that peptide P1 has potential as diagnostic tool in the genus Opisthorchis/Clonorchis. It should be further evaluated in combination with peptides derived from other O. viverrini antigens to increase its diagnostic power.

Citations

Citations to this article as recorded by

Production and immunological characterization of the novel single-chain variable fragment (scFv) antibodies against the epitopes on Opisthorchis viverrini cathepsin F (OvCatF)
Pongsakorn Martviset, Jeeraphong Thanongsaksrikul, Amornrat Geadkaew-Krenc, Salisa Chaimon, Kantaphon Glab-ampai, Wanlapa Chaibangyang, Phornphan Sornchuer, Potjanee Srimanote, Jittiporn Ruangtong, Parisa Prathaphan, Tonkla Taechadamrongtham, Nattaya Toru
Acta Tropica.2024; 254: 107199. CrossRef
Investigation of a Serine Protease Inhibitor Active in the Infectious Stage of the Human Liver Fluke Opisthorchis viverrini
Rosnanee Salang, Wansika Phadungsil, Amornrat Geadkaew-Krenc, Rudi Grams
Pathogens.2024; 13(8): 678. CrossRef
Production and Immunological Characterization of scFv Specific to Epitope of Opisthorchis viverrini Rhophilin-Associated Tail Protein 1-like (OvROPN1L)
Amornrat Geadkaew-Krenc, Dawid Krenc, Jeeraphong Thanongsaksrikul, Rudi Grams, Wansika Phadungsil, Kittirat Glab-ampai, Pathanin Chantree, Pongsakorn Martviset
Tropical Medicine and Infectious Disease.2023; 8(3): 160. CrossRef
Cystatins from the Human Liver Fluke Opisthorchis viverrini: Molecular Characterization and Functional Analysis
Amornrat Geadkaew-Krenc, Rudi Grams, Sinee Siricoon, Nanthawat Kosa, Dawid Krenc, Wansika Phadungsil, Pongsakorn Martviset
Pathogens.2023; 12(7): 949. CrossRef
Novel recombinant proteins and peptides from Clonorchis sinensis and Opisthorchis viverrini for liver fluke exposure ELISA
Sumathy Mohan, Mohan Natarajan, John G. Bruno
Biochemistry and Biophysics Reports.2023; 35: 101516. CrossRef
Screening of sperm antigen epitopes by phage display technique and its preliminary clinical application
Jin-Chun Lu, Yan-Mei Ge, Yuan-Hua Xu, Shan-Shan Tang, Yuan-Jiao Liang
Basic and Clinical Andrology.2022;[Epub] CrossRef

4,830 View
93 Download
6 Web of Science
Crossref

Original Article

4-Hydroxybenzaldehyde Restricts the Intracellular Growth of Toxoplasma gondii by Inducing SIRT1-Mediated Autophagy in Macrophages: Jina lee, Jae-Won Choi, Hye Young Han, Woo Sik Kim, Ha-Yeon Song, Eui-Baek Byun, Eui-Hong Byun, Young-Ha Lee, Jae-Min Yuk; Korean J Parasitol 2020;58(1):7-14.
Published online February 29, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.1.7

Abstract
PDF

Toxoplasma gondii is an intracellular protozoan parasite that infects approximately one third of the human popu- lation worldwide. Considering the toxicity and side effects of anti-toxoplasma medications, it is important to develop effec- tive drug alternatives with fewer and less severe off-target effects. In this study, we found that 4-hydroxybenzaldehyde (4- HBA) induced autophagy and the expression of NAD-dependent protein deacetylase sirtuin-1 (SIRT1) in primary murine bone marrow-derived macrophages (BMDMs). Interestingly, treatment of BMDMs with 4-HBA significantly reduced the number of macrophages infected with T. gondii and the proliferation of T. gondii in infected cells. This effect was impaired by pretreating the macrophages with 3-methyladenine or wortmannin (selective autophagy inhibitors) or with sirtinol or EX527 (SIRT1 inhibitors). Moreover, we found that pharmacological inhibition of SIRT1 prevented 4-HBA-mediated expres- sion of LC3-phosphatidylethanolamine conjugate (LC3-II) and the colocalization of T. gondii parasitophorous vacuoles with autophagosomes in BMDMs. These data suggest that 4-HBA promotes antiparasitic host responses by activating SIRT1- mediated autophagy, and 4-HBA might be a promising therapeutic alternative for the treatment of toxoplasmosis.

Citations

Citations to this article as recorded by

Impact of prenatal phthalate exposure on newborn metabolome and infant neurodevelopment
Susan S. Hoffman, Ziyin Tang, Anne Dunlop, Patricia A. Brennan, Thompson Huynh, Stephanie M. Eick, Dana B. Barr, Blake Rushing, Susan L. McRitchie, Susan Sumner, Kaitlin R. Taibl, Youran Tan, Parinya Panuwet, Grace E. Lee, Jasmin Eatman, Elizabeth J. Corw
Nature Communications.2025;[Epub] CrossRef
Neospora caninum infection specifically suppresses the expression of a host lncRNA XR_001919077.1 to facilitate parasite propagation by modulating host cell mitochondrial function and autophagy
Shan-Shan Zhao, De-Liang Tao, Jin-Ming Chen, Ming-Yi Zhang, Xin Yang, Jun-Ke Song, Qun Liu, Guang-Hui Zhao, Björn F. C. Kafsack
Microbiology Spectrum.2025;[Epub] CrossRef
Synthesis, structural characterization, in silico ADMET and molecular docking studies of a Schiff base derived from 4-hydroxybenzaldehyde and 4-aminobenzoic acid
Patryk Nowak, Artur Sikorski
New Journal of Chemistry.2025; 49(14): 5940. CrossRef
Metabolomic Plasma Profile of Chronic Obstructive Pulmonary Disease Patients
Carme Casadevall, Bella Agranovich, Cesar Jesse Enríquez-Rodríguez, Rosa Faner, Sergi Pascual-Guàrdia, Ady Castro-Acosta, Ramon Camps-Ubach, Judith Garcia-Aymerich, Esther Barreiro, Eduard Monsó, Luis Seijo, Juan José Soler-Cataluña, Salud Santos, Germán
International Journal of Molecular Sciences.2025; 26(10): 4526. CrossRef
The role of host autophagy in intracellular protozoan parasites diseases
Rafael Cardoso Maciel Costa Silva, Jhones Sousa Ribeiro, Thalita Santos de Moraes de Farias, Leonardo Holanda Travassos
Archives of Biochemistry and Biophysics.2024; 761: 110186. CrossRef
Structural diversity of cocrystals formed from acridine and two isomers of hydroxybenzaldehyde: 3-hydroxybenzaldehyde and 4-hydroxybenzaldehyde
Patryk Nowak, Artur Sikorski
RSC Advances.2023; 13(29): 20105. CrossRef
Protective effect of benzaldehyde combined with albendazole against brain injury induced by Angiostrongylus cantonensis infection in mice
Kuang-Yao Chen, Chien-Ju Cheng, Yi-Ju Chen, Cheng-Hsun Chiu, Lian-Chen Wang
International Journal of Antimicrobial Agents.2023; 62(5): 106963. CrossRef
Benzaldehyde Attenuates the Fifth Stage Larval Excretory–Secretory Product of Angiostrongylus cantonensis-Induced Injury in Mouse Astrocytes via Regulation of Endoplasmic Reticulum Stress and Oxidative Stress
Kuang-Yao Chen, Yi-Ju Chen, Chien-Ju Cheng, Kai-Yuan Jhan, Lian-Chen Wang
Biomolecules.2022; 12(2): 177. CrossRef
Neospora caninum infection induced mitochondrial dysfunction in caprine endometrial epithelial cells via downregulating SIRT1
De-Liang Tao, Shan-Shan Zhao, Jin-Ming Chen, Xi Chen, Xin Yang, Jun-Ke Song, Qun Liu, Guang-Hui Zhao
Parasites & Vectors.2022;[Epub] CrossRef
Modulation of autophagy as a therapeutic strategy for Toxoplasma gondii infection
Ao Cheng, Huanan Zhang, Baike Chen, Shengyao Zheng, Hongyi Wang, Yijia Shi, Siyao You, Ming Li, Liping Jiang
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
SIRT1 Promotes Host Protective Immunity against Toxoplasma gondii by Controlling the FoxO-Autophagy Axis via the AMPK and PI3K/AKT Signalling Pathways
Jina Lee, Jinju Kim, Jae-Hyung Lee, Yong Min Choi, Hyeonil Choi, Hwan-Doo Cho, Guang-Ho Cha, Young-Ha Lee, Eun-Kyeong Jo, Byung-Hyun Park, Jae-Min Yuk
International Journal of Molecular Sciences.2022; 23(21): 13578. CrossRef
The role of SIRT1 in the process of Toxoplasma gondii infection of RAW 264.7 macrophages
Kai Dong, Ziyang Jiang, Jianhui Zhang, Hanxiao Qin, Jianping Chen, Qiwei Chen
Frontiers in Microbiology.2022;[Epub] CrossRef
Recent Advances in the Roles of Autophagy and Autophagy Proteins in Host Cells During Toxoplasma gondii Infection and Potential Therapeutic Implications
Carlos S. Subauste
Frontiers in Cell and Developmental Biology.2021;[Epub] CrossRef
Moringa oleiferaextract promotes apoptosis-like death inToxoplasma gondiitachyzoitesin vitro
Letícia Nishi, Raquel Arruda da Silva Sanfelice, Bruna Taciane da Silva Bortoleti, Fernanda Tomiotto-Pellissier, Taylon Felipe Silva, Fernanda Ferreira Evangelista, Danielle Lazarin-Bidóia, Idessania Nazareth Costa, Wander Rogério Pavanelli, Ivete Conchon
Parasitology.2021; 148(12): 1447. CrossRef
Analysis of Phenolic Compounds in Coix-seed Reactive Derivatives(CRD) by Using Liquid Chromatography/Mass Spectrometry(LC-MS)
Nobutaka SUZUKI, Masahiko TAKINO, Hirotaka OKUWA-HAYASHI
Japanese Journal of Complementary and Alternative Medicine.2020; 17(1): 11. CrossRef
Urolithin-A attenuates neurotoxoplasmosis and alters innate response towards predator odor
Sijie Tan, Wen Han Tong, Ajai Vyas
Brain, Behavior, & Immunity - Health.2020; 8: 100128. CrossRef
The Host Autophagy During Toxoplasma Infection
Minmin Wu, Obed Cudjoe, Jilong Shen, Ying Chen, Jian Du
Frontiers in Microbiology.2020;[Epub] CrossRef

8,565 View
173 Download
17 Web of Science
Crossref

Brief Communications

Virus-like Particle Vaccine Containing Toxoplasma gondii Rhoptry Protein 13 Induces Protection against T. gondii ME49 Infection in Mice: Hae-Ji Kang, Ki-Back Chu, Su-Hwa Lee, Min-Ju Kim, Hyunwoo Park, Hui Jin, Fu-Shi Quan; Korean J Parasitol 2019;57(5):543-547.
Published online October 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.5.543

Abstract
PDF

Toxoplasma gondii can infect humans worldwide, causing serious diseases in pregnant women and immunocompromised individuals. T. gondii rhoptry protein 13 (ROP13) is known as one of the key proteins involved in host cell invasion. In this study, we generated virus-like particles (VLPs) vaccine expressing T. gondii rhoptry ROP13 and investigated VLPs vaccine efficacy in mice. Mice immunized with ROP13 VLPs vaccine elicited significantly higher levels of T. gondii-specific IgG, IgG1, IgG2a, and IgA antibody responses following boost immunization and challenge infection, whereas antibody inductions were insignificant upon prime immunization. Differing immunization routes resulted in differing antibody induction, as intranasal immunization (IN) induced greater antibody responses than intramuscular immunization (IM) after boost and challenge infection. IN immunization induced significantly higher levels of IgG and IgA antibody responses from feces, antibody-secreting cells (ASCs), CD4⁺ T, CD8⁺ T cells and germinal center B cell responses in the spleen compared to IM immunization. Compared to IM immunization, IN immunization resulted in significantly reduced cyst counts in the brain as well as lesser body weight loss, which contributed to better protection. All of the mice immunized through either route survived, whereas all na?ve control mice perished. These results indicate that the ROP13 VLPs vaccine could be a potential vaccine candidate against T. gondii infection.

Citations

Citations to this article as recorded by

A state-of-the-art methodology for high-throughput in silico vaccine discovery against protozoan parasites and exemplified with discovered candidates for Toxoplasma gondii
Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis
Scientific Reports.2023;[Epub] CrossRef
Recent progress in vaccine development targeting pre-clinical human toxoplasmosis
Ki-Back Chu, Fu-Shi Quan
Parasites, Hosts and Diseases.2023; 61(3): 231. CrossRef
Protective immunity induced by CpG ODN‐adjuvanted virus‐like particles containing Toxoplasma gondii proteins
Hae‐Ji Kang, Ki‐Back Chu, Min‐Ju Kim, Su‐Hwa Lee, Hyunwoo Park, Hui Jin, Eun‐Kyung Moon, Fu‐Shi Quan
Parasite Immunology.2021;[Epub] CrossRef
Protective Immunity Against Neospora caninum Infection Induced by 14-3-3 Protein in Mice
Shan Li, Nan Zhang, Shaoxiong Liu, Jianhua Li, Li Liu, Xiaocen Wang, Xin Li, Pengtao Gong, Xichen Zhang
Frontiers in Veterinary Science.2021;[Epub] CrossRef
Toxoplasma gondii virus‐like particle vaccination alleviates inflammatory response in the brain upon T gondii infection
Hae‐Ji Kang, Ki‐Back Chu, Su‐Hwa Lee, Min‐Ju Kim, Hyunwoo Park, Hui Jin, Eun‐Kyung Moon, Fu‐Shi Quan
Parasite Immunology.2020;[Epub] CrossRef
Evaluation of CpG-ODN-Adjuvanted Toxoplasma gondii Virus-Like Particle Vaccine upon One, Two, and Three Immunizations
Hae-Ji Kang, Ki-Back Chu, Min-Ju Kim, Hyunwoo Park, Hui Jin, Su-Hwa Lee, Eun-Kyung Moon, Fu-Shi Quan
Pharmaceutics.2020; 12(10): 989. CrossRef

6,881 View
91 Download
7 Web of Science
Crossref

Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016: Da-Won Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee; Korean J Parasitol 2019;57(5):531-536.
Published online October 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.5.531

Abstract
PDF

Cryptosporidium parvum and Giardia duodenalis are the main diarrhea-causing parasitic pathogens; however, their prevalence in Korea is unknown. Here, we conducted a survey to determine the prevalence and genotype distribution of these 2 pathogens causing acute diarrhea in 8,571 patients hospitalized in 17 Regional Institute of Health Environment sites in Korea, during 2013-2016. C. parvum and G. duodenalis were detected and genotyped by nested PCR, and the isolate were molecularly characterized by sequencing the glycoprotein 60 (Gp60) and β-giardin genes, respectively. The overall prevalence of C. parvum and G. duodenalis was 0.37% (n=32) and 0.55% (n=47), respectively, and both pathogens were more prevalent in children under 9 years old. Molecular epidemiological analysis showed that the C. parvum isolates belonged to the IIa family and were subtyped as IIaA13G2R1, IIaA14G2R1, IIaA15G2R1, and IIaA18G3R1. Analysis of the β-giardin gene fragment from G. duodenalis showed that all positive strains belong to assemblage A. This is the first report on the molecular epidemiology and subtyping of C. parvum and G. duodenalis in such a large number of diarrheal patients in Korea. These results highlight the need for continuous monitoring of these zoonotic pathogens and provide a basis for implementing control and prevention strategies. Further, the results might be useful for epidemiological investigation of the source of outbreak.

Citations

Citations to this article as recorded by

An Epidemiology Study on Distribution of Giardia lamblia in Incheon City
Su Jin Park, Ju Hee Kim, Hyeon-Jeong Go, Nam Yee Kim, Sung Suck Oh, Young Woo Gong, MunJu Kwon, Jeong-An Gim, Soo-Son Lim
Biomedical Science Letters.2025; 31(2): 218. CrossRef
Cryptosporidium and cryptosporidiosis: An update of Asian perspectives in humans, water and food, 2015–2025
Shahira Abdelaziz Ali Ahmed, Sonia Boughattas, Mohammad Reza Mahmoudi, Huma Khan, Simuzar Mamedova, Ardra Namboodiri, Frederick R. Masangkay, Panagiotis Karanis
Current Research in Parasitology & Vector-Borne Diseases.2025; 8: 100311. CrossRef
Detection and molecular characterization of Cryptosporidium spp. in dairy calves in Lisbon and Tagus Valley, Portugal
Mariana Louro, Ricardo Bexiga, Isabel Pereira da Fonseca, Jacinto Gomes
Veterinary Parasitology: Regional Studies and Reports.2024; 47: 100964. CrossRef
Epidemiological investigation of Cryptosporidium in children with diarrhea in middle Inner Mongolia, China
Wenhui Guo, Xinyu Xue, Ruifeng Li, Ru Liang, Zixuan Wang, Jiashan Qin, Chao Duan, Tieyun Chen, Xinlei Yan
Acta Tropica.2024; 254: 107177. CrossRef
Follow-up investigation into Cryptosporidium prevalence and transmission in Western European dairy farms
Sumaiya Hoque, Pedro Pinto, Cláudia A. Ribeiro, Evi Canniere, Yvonne Daandels, Martine Dellevoet, Anne Bourgeois, Ourida Hammouma, Paul Hunter, Eleni Gentekaki, Martin Kváč, Jérôme Follet, Anastasios D. Tsaousis
Veterinary Parasitology.2023; 318: 109920. CrossRef
Outbreak of severe diarrhea due to zoonotic Cryptosporidium parvum and C. xiaoi in goat kids in Chungcheongbuk-do, Korea
Ah-Young Kim, Badriah Alkathiri, Subin Lee, Kyung-Duk Min, Soochong Kim, Sang-Myeong Lee, Wan-Kyu Lee, Dongmi Kwak, Seung-Hun Lee
Parasitology Research.2023; 122(9): 2045. CrossRef
Emergence of zoonotic Cryptosporidium parvum in China
Yaqiong Guo, Una Ryan, Yaoyu Feng, Lihua Xiao
Trends in Parasitology.2022; 38(4): 335. CrossRef
Distribution of Cryptosporidium parvum subgenotypes in pre-weaned calves in Germany
Franziska Göhring, Matthias Lendner, Arwid Daugschies
Veterinary Parasitology: Regional Studies and Reports.2022; 36: 100806. CrossRef
Annual Report on the External Quality Assessment Scheme for Clinical Parasitology in Korea (2020–2021)
Jihu Jeon, Eun Jeong Won, Kyung Hwan Byeon, Yu Jeong Lee, Moon-Ju Kim, Myung Geun Shin, Jong Hee Shin
Journal of Laboratory Medicine and Quality Assurance.2022; 44(4): 185. CrossRef
Clinical cases of zoonotic Cryptosporidium parvum (subtype IIdA15G1) infections in Korean goats
J Kim, K Lee, SG Roh, HY Kim, JW Kim, BJ So, EJ Choi
Veterinární medicína.2022; 67(3): 156. CrossRef
Causes of acute gastroenteritis in Korean children between 2004 and 2019
Eell Ryoo
Clinical and Experimental Pediatrics.2021; 64(6): 260. CrossRef
Clinical and Laboratory Diagnosis of Cryptosporidiosis among Children with Acute Gastroenteritis at a Tertiary Hospital, Cairo, Egypt
Mohammad Ashraf Abdel Wahed, Yasmine Elsayed Abdelrahman Shehab, Hanan Mahmoud Abou-Seri, Yosra Mohamed Mohsen Awad
Journal of Tropical Pediatrics.2021;[Epub] CrossRef
Giardia duodenalis Virulence — “To Be, or Not To Be”
Raúl Argüello-García, M. Guadalupe Ortega-Pierres
Current Tropical Medicine Reports.2021; 8(4): 246. CrossRef
Cross-Border Investigations on the Prevalence and Transmission Dynamics of Cryptosporidium Species in Dairy Cattle Farms in Western Mainland Europe
Pedro Pinto, Cláudia A. Ribeiro, Sumaiya Hoque, Ourida Hammouma, Hélène Leruste, Sébastien Détriché, Evi Canniere, Yvonne Daandels, Martine Dellevoet, Janine Roemen, Anne Barbier Bourgeois, Martin Kváč, Jérôme Follet, Anastasios D. Tsaousis
Microorganisms.2021; 9(11): 2394. CrossRef
Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum
Léa Bordes, Pauline Houert, Damien Costa, Loïc Favennec, Corinne Vial-Novella, Francis Fidelle, Christelle Grisez, Françoise Prévot, Philippe Jacquiet, Romy Razakandrainibe
Parasite.2020; 27: 57. CrossRef
Report of the Korean Association of External Quality Assessment Service on Clinical Parasitology (2018–2019)
Suhak Jeon, Eun Jeong Won, Yu Jeong Lee, Moon-Ju Kim, Myung Geun Shin, Jong Hee Shin
Journal of Laboratory Medicine and Quality Assurance.2020; 42(4): 177. CrossRef

8,244 View
129 Download
14 Web of Science
Crossref

Low Levels of Polymorphisms and Negative Selection in Plasmodum knowlesi Merozoite Surface Protein 8 in Malaysian Isolates: Md Atique Ahmed, Hae-Ji Kang, Fu-Shi Quan; Korean J Parasitol 2019;57(4):445-450.
Published online August 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.4.445

Abstract
PDF

Human infections due to the monkey malaria parasite Plasmodium knowlesi is increasingly being reported from most Southeast Asian countries specifically Malaysia. The parasite causes severe and fatal malaria thus there is a need for urgent measures for its control. In this study, the level of polymorphisms, haplotypes and natural selection of full-length pkmsp8 in 37 clinical samples from Malaysian Borneo along with 6 lab-adapted strains were investigated. Low levels of polymorphism were observed across the full-length gene, the double epidermal growth factor (EGF) domains were mostly conserved, and non-synonymous substitutions were absent. Evidence of strong negative selection pressure in the non-EGF regions were found indicating functional constrains acting at different domains. Phylogenetic haplotype network analysis identified shared haplotypes and indicated geographical clustering of samples originating from Peninsular Malaysia and Malaysian Borneo. This is the first study to genetically characterize the full-length msp8 gene from clinical isolates of P. knowlesi from Malaysia; however, further functional characterization would be useful for future rational vaccine design.

Citations

Citations to this article as recorded by

Plasmodium knowlesi: the game changer for malaria eradication
Wenn-Chyau Lee, Fei Wen Cheong, Amirah Amir, Meng Yee Lai, Jia Hui Tan, Wei Kit Phang, Shahhaziq Shahari, Yee-Ling Lau
Malaria Journal.2022;[Epub] CrossRef

6,475 View
107 Download
1 Web of Science
Crossref

Detection of Human Anti-Trypanosoma cruzi Antibody with Recombinant Fragmented Ribosomal P Protein: Yeong Hoon Kim, Zhaoshou Yang, Jihoo Lee, Hye-Jin Ahn, Chom-Kyu Chong, Wagner Maricondi, Ronaldo F. Dias, Ho-Woo Nam; Korean J Parasitol 2019;57(4):435-437.
Published online August 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.4.435

Abstract
PDF

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and is endemic in many Latin American countries. Diagnosis is based on serologic testing and the WHO recommends two or more serological tests for confirmation. Acidic ribosomal P protein of T. cruzi showed strong reactivity against positive sera of patients, and we cloned the protein after fragmenting it to enhance its antigenicity and solubility. Twelve positive sera of Chagas disease patients were reacted with the fragmented ribosomal P protein using western blot. Detection rate and density for each fragment were determined. Fragments F1R1, F1R2, and F2R1 showed 100% rate of detection, and average density scoring of 2.00, 1.67, and 2.42 from a maximum of 3.0, respectively. Therefore, the F2R1 fragment of the ribosomal P protein of T. cruzi could be a promising antigen to use in the diagnosis of Chagas disease in endemic regions with high specificity and sensitivity.

Citations

Citations to this article as recorded by

In vitro diagnostic methods of Chagas disease in the clinical laboratory: a scoping review
Luis C. Ascanio, Savannah Carroll, Alberto Paniz-Mondolfi, Juan David Ramírez
Frontiers in Microbiology.2024;[Epub] CrossRef
A refined genome phage display methodology delineates the human antibody response in patients with Chagas disease
André Azevedo Reis Teixeira, Luis Rodriguez Carnero, Andréia Kuramoto, Fenny Hui Fen Tang, Carlos Hernique Gomes, Natalia Bueno Pereira, Léa Campos de Oliveira, Regina Garrini, Jhonatas Sirino Monteiro, João Carlos Setubal, Ester Cerdeira Sabino, Renata P
iScience.2021; 24(6): 102540. CrossRef

5,731 View
97 Download
2 Web of Science
Crossref

Original Articles

Cytokine Production in Cholangiocarcinoma Cells in Response to Clonorchis sinensis Excretory-Secretory Products and Their Putative Protein Components: Jhang Ho Pak, Ji-Yun Lee, Bo Young Jeon, Fuhong Dai, Won Gi Yoo, Sung-Jong Hong; Korean J Parasitol 2019;57(4):379-387.
Published online August 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.4.379

Abstract
PDF

Clonorchis sinensis is a carcinogenic human liver fluke that promotes hepatic inflammatory environments via direct contact or through their excretory-secretory products (ESPs), subsequently leading to cholangitis, periductal fibrosis, liver cirrhosis, and even cholangiocarcinoma (CCA). This study was conducted to examine the host inflammatory responses to C. sinensis ESPs and their putative protein components selected from C. sinensis expressed sequenced tag (EST) pool databases, including TGF-β receptor interacting protein 1(CsTRIP1), legumain (CsLeg), and growth factor binding protein 2 (CsGrb2). Treatment of CCA cells (HuCCT1) with the ESPs or bacterial recombinant C. sinensis proteins differentially promoted the secretion of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) as well as anti-inflammatory cytokines (IL-10, TGF-β1, and TGF-β2) in a time-dependent manner. In particular, recombinant C. sinensis protein treatment resulted in increase (at maximum) of ~7-fold in TGF-β1, ~30-fold in TGF-β2, and ~3-fold in TNF-α compared with the increase produced by ESPs, indicating that CsTrip1, CsLeg, and CsGrb2 function as strong inducers for secretion of these cytokines in host cells. These results suggest that C. sinensis ESPs contribute to the immunopathological response in host cells, leading to clonorchiasis-associated hepatobiliary abnormalities of greater severity.

Citations

Citations to this article as recorded by

Immunogenomics of cholangiocarcinoma
Virag Gehl, Colm J. O’Rourke, Jesper B. Andersen
Hepatology.2025; 82(2): 522. CrossRef
Clonorchis sinensis-driven hepatocarcinogenesis via E2F1-CD24 transcriptional axis: mechanistic and therapeutic implications
Wen-Min Lu, Jin Yan, Zhao-Ji Liu, Yong Wu, Qian-Ru Cui, Ji Feng, Yu Chen, Guang-Zhi Zhu, Tao Peng, Jing Zhou, Guo-Dong Lu
Parasites & Vectors.2025;[Epub] CrossRef
The crosstalk between cholangiocytes and hepatic stellate cells promotes the progression of epithelial-mesenchymal transition and periductal fibrosis during Clonorchis sinensis infection
Junyeong Yi, Ji Hoon Jeong, Jihee Won, Seok Chung, Jhang Ho Pak
Parasites & Vectors.2024;[Epub] CrossRef
Clonorchis sinensisinfection induces pathological changes in feline bile duct epithelium and alters biliary microbiota composition
Feng Li, Yanli Zhang, Chunfu Li, Fenqi Li, Baojiang Gan, Hong Yu, Jian Li, Xinyu Feng, Wei Hu
Parasite.2024; 31: 53. CrossRef
Up-regulation of MDSCs accumulation and Th2 biased response to co-stimulation of CsESP from Clonorchis sinensis and HBeAg in vitro
Qiannan Liu, Nian Wang, Hengchang Sun, Huimin Dong, Xuerong Li, Xinbing Yu, Yan Huang
Acta Tropica.2024; 260: 107405. CrossRef
The expression of sICAM-1 influenced by Clonorchis sinensis co-infection in CHB patients
J. Qiu, M. Shang, W. Li, H. Zhang, Y. Liao, H. Dong
Journal of Helminthology.2024;[Epub] CrossRef
Genus Bithynia: morphological classification to molecular identification
Guoyang Huang, Xiaohong Peng
Parasites & Vectors.2024;[Epub] CrossRef
Clonorchis sinensis granulin promotes malignant transformation of human intrahepatic biliary epithelial cells through interaction with M2 macrophages via regulation of STAT3 phosphorylation and the MEK/ERK pathway
Qing He, Xiaowen Pan, Yingxuan Yin, Anyuan Xu, Xueqing Yi, Yinjuan Wu, Xuerong Li
Parasites & Vectors.2023;[Epub] CrossRef
Recombinant protein EBI3 attenuates Clonorchis sinensis-induced liver fibrosis by inhibiting hepatic stellate cell activation in mice
Lei Zhao, Jia Li, Gang Mo, Deping Cao, Chun Li, Guoyang Huang, Liping Jiang, Gen Chen, Hongbing Yao, Xiaohong Peng
Parasites & Vectors.2023;[Epub] CrossRef
Cancerogenic parasites in veterinary medicine: a narrative literature review
Niccolò Fonti, Francesca Parisi, Francesca Mancianti, Giulia Freer, Alessandro Poli
Infectious Agents and Cancer.2023;[Epub] CrossRef
Molecular Mechanisms of Clonorchis sinensis-Host Interactions and Implications for Vaccine Development
Stephane Koda, Xing-Quan Zhu, Kui-Yang Zheng, Chao Yan
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Roles of microRNAs and Long Non-Coding RNAs Encoded by Parasitic Helminths in Human Carcinogenesis
Ana Gabriela Leija-Montoya, Javier González-Ramírez, Gustavo Martínez-Coronilla, María Esther Mejía-León, Mario Isiordia-Espinoza, Fausto Sánchez-Muñoz, Elda Georgina Chávez-Cortez, Viviana Pitones-Rubio, Nicolas Serafín-Higuera
International Journal of Molecular Sciences.2022; 23(15): 8173. CrossRef
The role of helminths in the development of non-communicable diseases
Yifan Wu, Megan Duffey, Saira Elizabeth Alex, Charlie Suarez-Reyes, Eva H. Clark, Jill E. Weatherhead
Frontiers in Immunology.2022;[Epub] CrossRef
Spirocerca lupi Proteomics and Its Role in Cancer Development: An Overview of Spirocercosis-Induced Sarcomas and Revision of Helminth-Induced Carcinomas
Catalina Porras-Silesky, María José Mejías-Alpízar, Javier Mora, Gad Baneth, Alicia Rojas
Pathogens.2021; 10(2): 124. CrossRef
The Dark Side of the Force: When the Immune System Is the Fuel of Tumor Onset
Elisabeth Digifico, Silvia Balinzo, Cristina Belgiovine
International Journal of Molecular Sciences.2021; 22(3): 1224. CrossRef
Tumor-associated macrophages in cholangiocarcinoma: complex interplay and potential therapeutic target
Menghua Zhou, Chaoqun Wang, Shounan Lu, Yanan Xu, Zihao Li, Hongchi Jiang, Yong Ma
EBioMedicine.2021; 67: 103375. CrossRef
Omega-Class Glutathione Transferases of Carcinogenic Liver Fluke, Clonorchis sinensis, Modulate Apoptosis and Differentiation of Host Cholangiocytes
Chun-Seob Ahn, Jeong-Geun Kim, Insug Kang, Yoon Kong
Antioxidants.2021; 10(7): 1017. CrossRef
Clonorchis sinensis and clonorchiasis
Byoung-Kuk Na, Jhang Ho Pak, Sung-Jong Hong
Acta Tropica.2020; 203: 105309. CrossRef
CD19+CD1dhiCD5hi B Cells Can Downregulate Malaria ITV Protection by IL-10 Secretion
Hongli Guan, Jiacong Peng, Liping Jiang, Gang Mo, Xiang Li, Xiaohong Peng
Frontiers in Public Health.2020;[Epub] CrossRef
Dopaminergic antagonists inhibit bile chemotaxis of adult Clonorchis sinensis and its egg production
Fuhong Dai, Jin-Ho Song, Yeon Pyo Hong, Xuelian Bai, Woon-Mok Sohn, Sung-Jong Hong, jong-Yil Chai
PLOS Neglected Tropical Diseases.2020; 14(3): e0008220. CrossRef
Identification and Analysis of the Tegument Protein and Excretory-Secretory Products of the Carcinogenic Liver Fluke Clonorchis sinensis
Yunliang Shi, Kai Yu, Anli Liang, Yan Huang, Fangqi Ou, Haiyan Wei, Xiaoling Wan, Yichao Yang, Weiyu Zhang, Zhihua Jiang
Frontiers in Microbiology.2020;[Epub] CrossRef
Bile Ductal Transcriptome Identifies Key Pathways and Hub Genes in Clonorchis sinensis-Infected Sprague-Dawley Rats
Won Gi Yoo, Jung-Mi Kang, Huong Giang Lê, Jhang Ho Pak, Sung-Jong Hong, Woon-Mok Sohn, Byoung-Kuk Na
The Korean Journal of Parasitology.2020; 58(5): 513. CrossRef

7,552 View
142 Download
23 Web of Science
Crossref

Characterization of Plasmodium berghei Homologues of T-cell Immunomodulatory Protein as a New Potential Candidate for Protecting against Experimental Cerebral Malaria: Ai Cui, Yucen Li, Xia Zhou, Lin Wang, Enjie Luo; Korean J Parasitol 2019;57(2):101-115.
Published online April 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.2.101

The pathogenesis of cerebral malaria is biologically complex and involves multi-factorial mechanisms such as microvascular congestion, immunopathology by the pro-inflammatory cytokine and endothelial dysfunction. Recent data have suggested that a pleiotropic T-cell immunomodulatory protein (TIP) could effectively mediate inflammatory cytokines of mammalian immune response against acute graft-versus-host disease in animal models. In this study, we identified a conserved homologue of TIP in Plasmodium berghei (PbTIP) as a membrane protein in Plasmodium asexual stage. Compared with PBS control group, the pathology of experimental cerebral malaria (ECM) in rPbTIP intravenous injection (i.v.) group was alleviated by the downregulation of pro-inflammatory responses, and rPbTIP i.v. group elicited an expansion of regulatory T-cell response. Therefore, rPbTIP i.v. group displayed less severe brain pathology and feverish mice in rPbTIP i.v. group died from ECM. This study suggested that PbTIP may be a novel promising target to alleviate the severity of ECM.

Citations

Citations to this article as recorded by

Malian children infected with Plasmodium ovale and Plasmodium falciparum display very similar gene expression profiles
Kieran Tebben, Salif Yirampo, Drissa Coulibaly, Abdoulaye K. Koné, Matthew B. Laurens, Emily M. Stucke, Ahmadou Dembélé, Youssouf Tolo, Karim Traoré, Amadou Niangaly, Andrea A. Berry, Bourema Kouriba, Christopher V. Plowe, Ogobara K. Doumbo, Kirsten E. Ly
PLOS Neglected Tropical Diseases.2023; 17(1): e0010802. CrossRef
Characterization of protein cargo of Echinococcus granulosus extracellular vesicles in drug response and its influence on immune response
María Celeste Nicolao, Christian Rodriguez Rodrigues, Magalí B. Coccimiglio, Camila Ledo, Guillermo H. Docena, Andrea C. Cumino
Parasites & Vectors.2023;[Epub] CrossRef

8,048 View
128 Download
2 Web of Science
Crossref

Brief Communications

Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep: Liu Tianli, Wang Xifeng, Tian Zhenzhong, Wang Lixia, Zhang Xingxing, Qiao Jun, Meng Qingling, Gong Shasha, Chen Ying, Cai Xuepeng; Korean J Parasitol 2019;57(1):61-67.
Published online February 26, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.1.61

Abstract
PDF

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

Citations

Citations to this article as recorded by

Rapid and non‐invasive detection of cystic echinococcosis in sheep based on serum fluorescence spectrum combined with machine learning algorithms
Shengke Xu, Wubulitalifu Dawuti, Maierhaba Maimaitiaili, Jingrui Dou, Malike Aizezi, Kalibixiati Aimulajiang, Xiaoyi Lü, Guodong Lü
Journal of Biophotonics.2024;[Epub] CrossRef
Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis
Ana Alice Maia Gonçalves, Anna Julia Ribeiro, Carlos Ananias Aparecido Resende, Carolina Alves Petit Couto, Isadora Braga Gandra, Isabelle Caroline dos Santos Barcelos, Jonatas Oliveira da Silva, Juliana Martins Machado, Kamila Alves Silva, Líria Souza Si
Microbial Cell Factories.2024;[Epub] CrossRef
Expression and serodiagnostic efficacy of a novel echinococcosis-specific recombinant fusion antigen rAgB8/1-Em18-Eg95
Yang Xianwei, Wang Tao, Chen Yin, Wang Wentao
Parasitology.2024; 151(13): 1458. CrossRef
Rapid and accurate screening of cystic echinococcosis in sheep based on serum Fourier‐transform infrared spectroscopy combined with machine learning algorithms
Wubulitalifu Dawuti, Jingrui Dou, Xiangxiang Zheng, Xiaoyi Lü, Hui Zhao, Lingfei Yang, Renyong Lin, Guodong Lü
Journal of Biophotonics.2023;[Epub] CrossRef
Evaluation of a novel Echinococcus granulosus recombinant fusion B-EpC1 antigen for the diagnosis of human cystic echinococcosis using indirect ELISA in comparison with a commercial diagnostic ELISA kit
Enayat Darabi, Elahe Motevaseli, Mehdi Mohebali, Mohammad Bagher Rokni, Mohammad Reza Khorramizadeh, Farzaneh Zahabiun, Soudabeh Heidari, Eshrat Beigom Kia
Experimental Parasitology.2022; 240: 108339. CrossRef
Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line
Andrea Maglioco, Jorge Gentile, Melisa S. Barbery Venturi, Oscar Jensen, Claudia Hernández, María Laura Gertiser, Verónica Poggio, Gabriela Canziani, Alicia Graciela Fuchs
Parasite Immunology.2019;[Epub] CrossRef

7,520 View
130 Download
6 Web of Science
Crossref

Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis: Tahali Mendoza-Oliveros, Victor Arana-Arga?ez, Leidi C. Alvare?z-Sa?nchez, Julio Lara-Riegos, Mari?a Elizbeth Alvare?z-Sa?nchez, Julio C. Torres-Romero; Korean J Parasitol 2019;57(1):33-38.
Published online February 26, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.1.33

Abstract
PDF

Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis, which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis, which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTRPV), as a promising immunogen in BALB/c mice. First, TvTRPV was cloned and expressed as a recombinant protein in Escherichia coli BL21 cells and purified by nickel affinity. Next, BALB/c mice were immunized and the antibody levels in mice serum and cytokines from the supernatant of macrophages and from co-culture systems were evaluated. Recombinant TvTRPV triggered high levels of specific total IgG in sera from the immunized mice. Also, a statistically significant increase of cytokines: IL-1β, IL-6, and TNF-α after stimulation with the corresponding antigens in vitro, was identified. Moreover, co-cultures using CD4+ T cells from immunized mice were able to identify higher levels of IL-10 and IFN-γ. These results were useful to validate the immunogenicity of TvTRPV in BALB/c mice, where IL-10-IFN-γ-secreting cells could play a role in infection control, supporting the potential of TvTRPV as a promising target for vaccine against T. vaginalis.

Citations

Citations to this article as recorded by

Exposure of Tritrichomonas foetus to sublethal doses of metronidazole induces a specific proinflammatory response in murine macrophages
Emanuel Ceballos‐Góngora, Julio César Torres‐Romero, Victor Ermilo Arana‐Argáez, María Elizbeth Alvarez‐Sánchez, Karla Acosta‐Viana, Antonio Euan‐Canto, Leidi Cristal Alvarez‐Sánchez
Journal of Eukaryotic Microbiology.2024;[Epub] CrossRef
Activation of murine macrophages by membrane proteins from Tritrichomonas foetus grown on iron‐ and calcium‐rich conditions
Antonio Euan‐Canto, Julio César Torres‐Romero, María Elizbeth Alvarez‐Sánchez, Victor Ermilo Arana‐Argáez, Karla Acosta‐Viana, Emanuel Ceballos‐Góngora, Laura Vázquez‐Carrillo, Leidi Alvarez‐Sánchez
Parasite Immunology.2024;[Epub] CrossRef
In Vitro Activation of Macrophages by an MHC Class II-restricted Trichomonas Vaginalis TvZIP8-derived Synthetic Peptide
Victor Ermilo Arana-Argáez, Emanuel Ceballos-Góngora, María Elizbeth Alvarez-Sánchez, Antonio Euan-Canto, Julio Lara-Riegos, Julio César Torres-Romero
Immunological Investigations.2022; 51(1): 88. CrossRef
Local cytokine/chemokine profiles in BALB/c and C57BL/6 mice in response to T. vaginalis infection
Sonal Yadav, Vivek Verma, Rakesh Singh Dhanda, Sumeeta Khurana, Manisha Yadav
Experimental Parasitology.2022; 239: 108287. CrossRef
The molecular characterization and immune protection of adhesion protein 65 (AP65) of Trichomonas vaginalis
Zhenchao Zhang, Xiaoxiao Song, Zhengbo Zhang, Haoran Li, Yujuan Duan, Hao Zhang, Haoran Lu, Chengyang Luo, Mingyong Wang
Microbial Pathogenesis.2021; 152: 104750. CrossRef
The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)
Zhenchao Zhang, Yuhua Li, Shuai Wang, Lixia Hao, Yunqing Zhu, Haoran Li, Xiaoxiao Song, Yujuan Duan, Yuhui Sang, Pucheng Wu, Xiangrui Li
Frontiers in Microbiology.2020;[Epub] CrossRef

7,172 View
117 Download
8 Web of Science
Crossref

Complete Mitochondrial Genome of the Chagas Disease Vector, Triatoma rubrofasciata: Li Dong, Xiaoling Ma, Mengfei Wang, Dan Zhu, Yuebiao Feng, Yi Zhang, Jingwen Wang; Korean J Parasitol 2018;56(5):515-519.
Published online October 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.5.515

Abstract
PDF

Triatoma rubrofasciata is a wide-spread vector of Chagas disease in Americas. In this study, we completed the mitochondrial genome sequencing of T. rubrofasciata. The total length of T. rubrofasciata mitochondrial genome was 17,150 bp with the base composition of 40.4% A, 11.6% G, 29.4% T and 18.6% C. It included 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and one control region. We constructed a phylogenetic tree on the 13 protein-coding genes of T. rubrofasciata and other 13 closely related species to show their phylogenic relationship. The determination of T. rubrofasciata mitogenome would play an important role in understanding the genetic diversity and evolution of triatomine bugs.

Citations

Citations to this article as recorded by

The mitogenome of Triatoma brasiliensis brasiliensis (Hemiptera: Reduviidae), the main Chagas disease vector in the semi-arid region of northeastern Brazil
Carlos E. Almeida, Lifeng Du, Jingwen Wang, Dayane Pires-Silva, Elaine Folly-Ramos, Myrian Harry, Cleber Galvão
Parasites & Vectors.2025;[Epub] CrossRef
Accidental importation of the vector of Chagas disease, Triatoma rubrofasciata (De Geer, 1773) (Hemiptera, Reduviidae, Triatominae), in Europe
Francisco Collantes, Juan Francisco Campos-Serrano, Ignacio Ruiz-Arrondo
Journal of Vector Ecology.2023;[Epub] CrossRef
Variation in the Mitochondrial Genome of the Chagas Disease Vector Triatoma infestans (Hemiptera: Reduviidae)
Cintia Judith Fernández, Beatriz Alicia García
Neotropical Entomology.2022; 51(3): 483. CrossRef
The Complete Nucleotide Sequence and Gene Organization of the Mitochondrial Genome of Triatoma boliviana (Hemiptera, Reduviidae, Triatominae) and Phylogenetic Comparisons
Sebastián Pita, Pablo Mora, Mirko Rojas-Cortez, Teresa Palomeque, Pedro Lorite, Francisco Panzera
Arthropoda.2022; 1(1): 3. CrossRef
Modelling the climatic suitability of Chagas disease vectors on a global scale
Fanny E Eberhard, Sarah Cunze, Judith Kochmann, Sven Klimpel
eLife.2020;[Epub] CrossRef
Phylogeny of the North-Central American clade of blood-sucking reduviid bugs of the tribe Triatomini (Hemiptera: Triatominae) based on the mitochondrial genome
Magali Aguilera-Uribe, Rubi Nelsi Meza-Lázaro, Troy J. Kieran, Carlos N. Ibarra-Cerdeña, Alejandro Zaldívar-Riverón
Infection, Genetics and Evolution.2020; 84: 104373. CrossRef
Mitochondrial genomes of three kissing bugs (Reduviidae: Triatominae) and their phylogenetic implications
Yisheng Zhao, Manjie Jiang, Yunfei Wu, Fan Song, Wanzhi Cai, Hu Li
International Journal of Biological Macromolecules.2019; 134: 36. CrossRef
Mitogenome analysis of Indian isolate of Rhipicephalus microplus clade A sensu ( ): A first report from Maritime South-East Asia
Arun Kumar De, Ramachandran Muthiyan, Perumal Ponraj, K. Muniswamy, Jai Sunder, A. Kundu, D. Karunakaran, Zachariah George, M.S. Kundu, S.K. Zamir Ahmed, Dhruba Malakar, D. Bhattacharya
Mitochondrion.2019; 49: 135. CrossRef
Biological attributes of the kissing bug Triatoma rubrofasciata from Vietnam
Ho Viet Hieu, Le Thanh Do, Sebastián Pita, Hoang Ha, Pham Thi Khoa, Pham Anh Tuan, Ta Phuong Mai, Ngo Giang Lien, Francisco Panzera
Parasites & Vectors.2019;[Epub] CrossRef

8,638 View
110 Download
9 Web of Science
Crossref

Original Articles

Virus-Like Particles Expressing Toxoplasma gondii Rhoptry Protein 18 Induces Better Protection Than Rhoptry Protein 4 against T. gondii Infection: Hae-Ji Kang, Su-Hwa Lee, Ki-Back Chu, Dong-Hun Lee, Fu-Shi Quan; Korean J Parasitol 2018;56(5):429-435.
Published online October 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.5.429

Abstract
PDF

Toxoplasma gondii is a ubiquitous protozoan parasite responsible for causing toxoplasmosis. Preventive measures for toxoplasmosis are currently lacking and as such, development of novel vaccines are of urgent need. In this study, we generated 2 virus-like particles (VLPs) vaccines expressing T. gondii rhoptry protein 4 (ROP4) or rhoptry protein 18 (ROP18) using influenza matrix protein (M1) as a core protein. Mice were intranasally immunized with VLPs vaccines and after the last immunization, mice were challenged with ME49 cysts. Protective efficacy was assessed and compared by determining serum antibody responses, body weight changes and the reduction of cyst counts in the brain. ROP18 VLPs-immunized mice induced greater levels of IgG and IgA antibody responses than those immunized with ROP4 VLPs. ROP18 VLPs immunization significantly reduced body weight loss and the number of brain cysts in mice compared to ROP4 VLPs post-challenge. These results indicate that T. gondii ROP18 VLPs elicited better protective efficacy than ROP4 VLPs, providing important insight into vaccine design strategy.

Citations

Citations to this article as recorded by

Evaluation of a DNA vector plasmid encoding a partial rop18 gene from toxoplasma gondii in domestic cats as a vaccine candidate
Ana Flávia Minutti, João Pedro Sasse, Ana Clécia dos Santos Silva, Thais Agostinho Martins, Valentina Martinez, Beatriz de Souza Lima Nino, Fernando de Souza Rodrigues, Luiz Daniel de Barros, João Luis Garcia
Vaccine.2025; 54: 126965. CrossRef
Applications of virus-like particles in the prevention of protozoan parasite infection
Ki Back Chu, Fu-Shi Quan
Nanomedicine.2025; 20(13): 1573. CrossRef
Recent progress in vaccine development targeting pre-clinical human toxoplasmosis
Ki-Back Chu, Fu-Shi Quan
Parasites, Hosts and Diseases.2023; 61(3): 231. CrossRef
Orally Administrated Recombinant Vaccinia Virus Displaying ROP4 Induces Protection against Toxoplasma gondii Challenge Infection
Keon-Woong Yoon, Ki-Back Chu, Hae-Ji Kang, Min-Ju Kim, Gi-Deok Eom, Fu-Shi Quan
Vaccines.2022; 10(2): 152. CrossRef
Protective immunity induced by CpG ODN‐adjuvanted virus‐like particles containing Toxoplasma gondii proteins
Hae‐Ji Kang, Ki‐Back Chu, Min‐Ju Kim, Su‐Hwa Lee, Hyunwoo Park, Hui Jin, Eun‐Kyung Moon, Fu‐Shi Quan
Parasite Immunology.2021;[Epub] CrossRef
A peptide originated from Toxoplasma gondii microneme 8 displaying serological evidence to differentiate recent from chronic human infection
Silas Silva Santana, Vinícius Fernandes Paiva, Fernando Reis Carvalho, Heber Leão Silva Barros, Tamires Lopes Silva, Patrício Silva Cardoso Barros, Ana Cláudia Arantes Marquez Pajuaba, Geisa Baptista Barros, Reynaldo Dietze, Tiago Wilson Patriarca Mineo,
Parasitology International.2021; 84: 102394. CrossRef
Evaluation of CpG-ODN-Adjuvanted Toxoplasma gondii Virus-Like Particle Vaccine upon One, Two, and Three Immunizations
Hae-Ji Kang, Ki-Back Chu, Min-Ju Kim, Hyunwoo Park, Hui Jin, Su-Hwa Lee, Eun-Kyung Moon, Fu-Shi Quan
Pharmaceutics.2020; 12(10): 989. CrossRef
Previous Infection with Plasmodium berghei Confers Resistance to Toxoplasma gondii Infection in Mice
Dong-Hun Lee, Ki-Back Chu, Hae-Ji Kang, Su-Hwa Lee, Fu-Shi Quan
The Korean Journal of Parasitology.2019; 57(2): 93. CrossRef
Influenza Virus-Like Particles Presenting both Toxoplasma gondii ROP4 and ROP13 Enhance Protection against T. gondii Infection
Hae-Ji Kang, Su-Hwa Lee, Min-Ju Kim, Ki-Back Chu, Dong-Hun Lee, Manika Chopra, Hyo-Jick Choi, Hyunwoo Park, Hui Jin, Fu-Shi Quan
Pharmaceutics.2019; 11(7): 342. CrossRef
Virus-like particles containing multiple antigenic proteins of Toxoplasma gondii induce memory T cell and B cell responses
Su-Hwa Lee, Ki-Back Chu, Hae-Ji Kang, Fu-Shi Quan, Paulo Lee Ho
PLOS ONE.2019; 14(8): e0220865. CrossRef

8,426 View
135 Download
9 Web of Science
Crossref

Genetic Diversity and Natural Selection in 42 kDa Region of Plasmodium vivax Merozoite Surface Protein-1 from China-Myanmar Endemic Border: Xia Zhou, Ernest Tambo, Jing Su, Qiang Fang, Wei Ruan, Jun-Hu Chen, Ming-Bo Yin, Xiao-Nong Zhou; Korean J Parasitol 2017;55(5):473-480.
Published online October 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.5.473

Plasmodium vivax merozoite surface protein-1 (PvMSP1) gene codes for a major malaria vaccine candidate antigen. However, its polymorphic nature represents an obstacle to the design of a protective vaccine. In this study, we analyzed the genetic polymorphism and natural selection of the C-terminal 42 kDa fragment within PvMSP1 gene (Pv MSP1₄₂) from 77 P. vivax isolates, collected from imported cases of China-Myanmar border (CMB) areas in Yunnan province and the inland cases from Anhui, Yunnan, and Zhejiang province in China during 2009-2012. Totally, 41 haplotypes were identified and 30 of them were new haplotypes. The differences between the rates of non-synonymous and synonymous mutations suggest that PvMSP1₄₂ has evolved under natural selection, and a high selective pressure preferentially acted on regions identified of PvMSP1₃₃. Our results also demonstrated that PvMSP1₄₂ of P. vivax isolates collected on China-Myanmar border areas display higher genetic polymorphisms than those collected from inland of China. Such results have significant implications for understanding the dynamic of the P. vivax population and may be useful information towards China malaria elimination campaign strategies.

Citations

Citations to this article as recorded by

Genetic diversity and molecular evolution of Plasmodium vivax Duffy Binding Protein and Merozoite Surface Protein-1 in northwestern Thailand
Parsakorn Tapaopong, Gustavo da Silva, Sittinont Chainarin, Chayanut Suansomjit, Khajohnpong Manopwisedjaroen, Liwang Cui, Cristian Koepfli, Jetsumon Sattabongkot, Wang Nguitragool
Infection, Genetics and Evolution.2023; 113: 105467. CrossRef
Spatiotemporal Changes in Plasmodium vivax msp142 Haplotypes in Southern Mexico: From the Control to the Pre-Elimination Phase
Alejandro Flores-Alanis, Lilia González-Cerón, Frida Santillán-Valenzuela, Cecilia Ximenez, Marco A. Sandoval-Bautista, Rene Cerritos
Microorganisms.2022; 10(1): 186. CrossRef
Genetic polymorphisms in genes associated with drug resistance in Plasmodium vivax parasites from northeastern Myanmar
Fang Huang, Shigang Li, Peng Tian, Lahpai Ja Seng Pu, Yanwen Cui, Hui Liu, Lianzhi Yang, Dahidam Yaw Bi
Malaria Journal.2022;[Epub] CrossRef
Diversity and natural selection of Merozoite surface Protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
Xiang Ting Goh, Yvonne A.L. Lim, Ping Chin Lee, Veeranoot Nissapatorn, Kek Heng Chua
Molecular and Biochemical Parasitology.2021; 244: 111390. CrossRef
Genetic diversity of Merozoite surface protein 1–42 (MSP1-42) fragment of Plasmodium vivax from Indonesian isolates: Rationale implementation of candidate MSP1 vaccine
E. Elsa Herdiana Murhandarwati, E. Henny Herningtyas, Puspawati Puspawati, Fridolina Mau, Shen-Bo Chen, Hai-Mo Shen, Jun-Hu Chen
Infection, Genetics and Evolution.2020; 85: 104573. CrossRef
Exploration of Plasmodium vivax merozoite surface proteins 1 and 7 genetic diversity in Brazilian Amazon and Rio de Janeiro Atlantic Forest
Natália Ketrin Almeida-de-Oliveira, Rebecca Abreu-Fernandes, Aline Rosa Lavigne, Anielle Pina-Costa, Daiana de Souza Perce-da-Silva, Marcos Catanho, Átila Duque Rossi, Patrícia Brasil, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz
Infection, Genetics and Evolution.2020; 86: 104592. CrossRef
Genetic polymorphism and natural selection in the C-terminal 42 kDa region of merozoite surface protein-1 (MSP-1) among Plasmodium knowlesi samples from Malaysia
Nan Jiun Yap, Indra Vythilingam, Boon Peng Hoh, Xiang Ting Goh, Azdayanti Muslim, Romano Ngui, Yamuna Rajoo, Seow Huey Choy, Timothy William, Tsin Wen Yeo, Yvonne Ai-Lian Lim
Parasites & Vectors.2018;[Epub] CrossRef

10,826 View
151 Download
7 Web of Science
Crossref

Brief Communication

Characterization of Echinostoma cinetorchis endoribonuclease, RNase H: Sung-Bin Lim, Seok Ho Cha, Seung Jegal, Hojong Jun, Seo Hye Park, Bo-Young Jeon, Jhang Ho Pak, Young Yil Bakh, Tong-Soo Kim, Hyeong-Woo Lee; Korean J Parasitol 2017;55(4):451-455.
Published online August 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.4.451

Abstract
PDF

Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3'-O-P bond of RNA in a DNA-RNA duplex, producing 3'-hydroxyl and 5'-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.

8,049 View
110 Download

Original Articles

Influenza M1 Virus-Like Particles Consisting of Toxoplasma gondii Rhoptry Protein 4: Su-Hwa Lee, Dong-Hun Lee, Ying Piao, Eun-Kyung Moon, Fu-Shi Quan; Korean J Parasitol 2017;55(2):143-148.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.143

Abstract
PDF

Toxoplasma gondii infections occur throughout the world, and efforts are needed to develop various vaccine candidates expressing recombinant protein antigens. In this study, influenza matrix protein (M1) virus-like particles (VLPs) consisting of T. gondii rhoptry antigen 4 (ROP4 protein) were generated using baculovirus (rBV) expression system. Recombinant ROP4 protein with influenza M1 were cloned and expressed in rBV. SF9 insect cells were coinfected with recombinant rBVs expressing T. gondii ROP4 and influenza M1. As the results, influenza M1 VLPs showed spherical shapes, and T. gondii ROP4 protein exhibited as spikes on VLP surface under transmission electron microscopy (TEM). The M1 VLPs resemble virions in morphology and size. We found that M1 VLPs reacted with antibody from T. gondii-infected mice by western blot and ELISA. This study demonstrated that T. gondii ROP4 protein can be expressed on the surface of influenza M1 VLPs and the M1 VLPs containing T. gondii ROP4 reacted with T. gondii-infected sera, indicating the possibility that M1 VLPs could be used as a coating antigen for diagnostic and/or vaccine candidate against T. gondii infection.

Citations

Citations to this article as recorded by

IgM Antibody Detection as a Diagnostic Marker for Acute Toxoplasmosis: Current Status of Studies and Main Limitations
Karolina Sołowińska, Lucyna Holec-Gąsior
Antibodies.2025; 14(2): 44. CrossRef
Protective immunity induced by CpG ODN‐adjuvanted virus‐like particles containing Toxoplasma gondii proteins
Hae‐Ji Kang, Ki‐Back Chu, Min‐Ju Kim, Su‐Hwa Lee, Hyunwoo Park, Hui Jin, Eun‐Kyung Moon, Fu‐Shi Quan
Parasite Immunology.2021;[Epub] CrossRef
Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen
Min-Ju Kim, Jie Mao, Hae-Ji Kang, Ki-Back Chu, Fu-Shi Quan
The Korean Journal of Parasitology.2021; 59(6): 565. CrossRef
Virus-like particle vaccine displaying Toxoplasma gondii apical membrane antigen 1 induces protection against T. gondii ME49 infection in mice
Min-Ju Kim, Su-Hwa Lee, Hae-Ji Kang, Ki-Back Chu, Hyunwoo Park, Hui Jin, Eun-Kyung Moon, Sung Soo Kim, Fu-Shi Quan
Microbial Pathogenesis.2020; 142: 104090. CrossRef
Toxoplasma gondii virus‐like particle vaccination alleviates inflammatory response in the brain upon T gondii infection
Hae‐Ji Kang, Ki‐Back Chu, Su‐Hwa Lee, Min‐Ju Kim, Hyunwoo Park, Hui Jin, Eun‐Kyung Moon, Fu‐Shi Quan
Parasite Immunology.2020;[Epub] CrossRef
Previous Infection with Plasmodium berghei Confers Resistance to Toxoplasma gondii Infection in Mice
Dong-Hun Lee, Ki-Back Chu, Hae-Ji Kang, Su-Hwa Lee, Fu-Shi Quan
The Korean Journal of Parasitology.2019; 57(2): 93. CrossRef
Virus-Like Particles Expressing Toxoplasma gondii Rhoptry Protein 18 Induces Better Protection Than Rhoptry Protein 4 against T. gondii Infection
Hae-Ji Kang, Su-Hwa Lee, Ki-Back Chu, Dong-Hun Lee, Fu-Shi Quan
The Korean Journal of Parasitology.2018; 56(5): 429. CrossRef

8,929 View
161 Download
7 Web of Science
Crossref

Differential Protein Expressions in Virus-Infected and Uninfected Trichomonas vaginalis: Ding He, Gong Pengtao, Yang Ju Li Jianhua, Li He Zhang Guocai, Zhang Xichen; Korean J Parasitol 2017;55(2):121-128.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.121

Abstract
PDF

Protozoan viruses may influence the function and pathogenicity of the protozoa. Trichomonas vaginalis is a parasitic protozoan that could contain a double stranded RNA (dsRNA) virus, T. vaginalis virus (TVV). However, there are few reports on the properties of the virus. To further determine variations in protein expression of T. vaginalis, we detected 2 strains of T. vaginalis; the virus-infected (V⁺) and uninfected (V^-) isolates to examine differentially expressed proteins upon TVV infection. Using a stable isotope N-terminal labeling strategy (iTRAQ) on soluble fractions to analyze proteomes, we identified 293 proteins, of which 50 were altered in V⁺ compared with V^- isolates. The results showed that the expression of 29 proteins was increased, and 21 proteins decreased in V⁺ isolates. These differentially expressed proteins can be classified into 4 categories: ribosomal proteins, metabolic enzymes, heat shock proteins, and putative uncharacterized proteins. Quantitative PCR was used to detect 4 metabolic processes proteins: glycogen phosphorylase, malate dehydrogenase, triosephosphate isomerase, and glucose-6-phosphate isomerase, which were differentially expressed in V⁺ and V^- isolates. Our findings suggest that mRNA levels of these genes were consistent with protein expression levels. This study was the first which analyzed protein expression variations upon TVV infection. These observations will provide a basis for future studies concerning the possible roles of these proteins in host-parasite interactions.

Citations

Citations to this article as recorded by

Role of parasite extracellular vesicles/exosomes in the interaction between hosts and virus-infected flagellate protozoa: Progress and prospects
Lu Li, Xiaocen Wang, Jianhua Li, Xichen Zhang, Xin Li, Nan Zhang, Lili Cao, Pengtao Gong
Animals and Zoonoses.2025;[Epub] CrossRef
Trichomonas vaginalis Virus: Current Insights and Emerging Perspectives
Keonte J. Graves, Jan Novak, Christina A. Muzny
Viruses.2025; 17(7): 898. CrossRef
Presence of Protozoan Viruses in Vaginal Samples from Pregnant Women and Their Association with Trichomoniasis
Gegham Ghardyan, Lusine Abrahamyan, Karen Julhakyan, Hakob Davtyan, Norayr Martirosyan, Elina Arakelova, Hranush Avagyan, Sona Hakobyan, Tigranuhi Vardanyan, Naira Karalyan, Zaven Karalyan
Pathogens.2025; 14(8): 764. CrossRef
Identification of an uncharacterized protein as a novel regulator of Giardia lamblia virus (GLV) infection in Giardia duodenalis
Zhiteng Zhao, Lili Cao, Jianqi Yuan, Shaoxiong Liu, Min Sun, Xin Li, Xiaocen Wang, Nan Zhang, Jianhua Li, Xichen Zhang, Pengtao Gong, Monique M. van Oers
Journal of Virology.2025;[Epub] CrossRef
The consequences of viral infection on protists
Victoria Fulgencio Queiroz, Juliana Miranda Tatara, Bruna Barbosa Botelho, Rodrigo Araújo Lima Rodrigues, Gabriel Magno de Freitas Almeida, Jonatas Santos Abrahao
Communications Biology.2024;[Epub] CrossRef
ՏՐԻԽՈՄՈՆԱՍ ՎԱԳԻՆԱԼԻՍ ՎԻՐՈՒՍԻ (TVV) ԱԶԴԵՑՈՒԹՅՈՒՆԸ ԿԱՆԱՆՑ ՄԻԶԱՍԵՌԱԿԱՆ ՏՐԻԽՈՄՈՆԻԱԶԻ ՎՐԱ
G.K. Ghardyan
MEDICINE, SCIENCE AND EDUCATION.2024; (37): 70. CrossRef
Microbial Matryoshka: Addressing the Relationship between Pathogenic Flagellated Protozoans and Their RNA Viral Endosymbionts (Family Totiviridae)
Alexandra Ibañez-Escribano, Maria Teresa Gomez-Muñoz, Marta Mateo, Cristina Fonseca-Berzal, Esperanza Gomez-Lucia, Raquel Garcia Perez, Jose M. Alunda, Javier Carrion
Veterinary Sciences.2024; 11(7): 321. CrossRef
Sandwich enzyme-linked aptamer-based assay for the detection of Trichomonas vaginalis
Christine Aubrey C. Justo, Miriam Jauset-Rubio, Marketa Svobodova, Vasso Skouridou, Piet Cools, Guy Mulinganya, Alexandra Ibáñez-Escribano, Windell L. Rivera, Ciara K. O'Sullivan
Analytical Biochemistry.2024; 695: 115656. CrossRef
PROTOZOONLARIN VİRAL ENDOSİMBİYONTLARI
Ayşegül DAMLAPINAR, Kader YILDIZ
Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni.2023; 14(1): 25. CrossRef
Multiple Regulations of Parasitic Protozoan Viruses: A Double-Edged Sword for Protozoa
Zhiteng Zhao, Xin Li, Nan Zhang, Jianhua Li, Na Zhao, Mengyao Gao, Xichen Zhang, Xiaocen Wang, Panpan Zhao, Lu Li, Min Sun, Lili Cao, Pengtao Gong, Vinayaka R. Prasad
mBio.2023;[Epub] CrossRef
Cytidine nucleoside analog is an effective antiviral drug against Trichomonasvirus
Ravi Kumar Narayanasamy, Petr Rada, Alois Zdrha, Marc van Ranst, Johan Neyts, Jan Tachezy
Journal of Microbiology, Immunology and Infection.2022; 55(2): 191. CrossRef
Viral endosymbiotic infection of protozoan parasites: How it influences the development of cutaneous leishmaniasis
Andrea Lafleur, Martin Olivier, Neal Silverman
PLOS Pathogens.2022; 18(11): e1010910. CrossRef
Viruses of protozoan parasites and viral therapy: Is the time now right?
Paul Barrow, Jean Claude Dujardin, Nicolas Fasel, Alex D. Greenwood, Klaus Osterrieder, George Lomonossoff, Pier Luigi Fiori, Robert Atterbury, Matteo Rossi, Marco Lalle
Virology Journal.2020;[Epub] CrossRef
Trichomonas vaginalis: pathogenesis and its role in cervical cancer
José Núñez-Troconis
Investigación Clínica.2020; 61(4): 349. CrossRef
Trichomonas vaginalis virus: a review of the literature
KJ Graves, AP Ghosh, PJ Kissinger, CA Muzny
International Journal of STD & AIDS.2019; 30(5): 496. CrossRef
Trichomonas vaginalis Virus Among Women With Trichomoniasis and Associations With Demographics, Clinical Outcomes, and Metronidazole Resistance
Keonte J Graves, Arindam P Ghosh, Norine Schmidt, Peter Augostini, W Evan Secor, Jane R Schwebke, David H Martin, Patricia J Kissinger, Christina A Muzny
Clinical Infectious Diseases.2019; 69(12): 2170. CrossRef

9,665 View
180 Download
14 Web of Science
Crossref

Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii: Eun-Kyung Moon, Hyun-Hee Kong, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan; Korean J Parasitol 2017;55(2):109-114.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.109

Abstract
PDF

Protein arginine methyltransferase (PRMT) is an important epigenetic regulator in eukaryotic cells. During encystation, an essential process for Acanthamoeba survival, the expression of a lot of genes involved in the encystation process has to be regulated in order to be induced or inhibited. However, the regulation mechanism of these genes is yet unknown. In this study, the full-length 1,059 bp cDNA sequence of Acanthamoeba castellanii PRMT1 (AcPRMT1) was cloned for the first time. The AcPRMT1 protein comprised of 352 amino acids with a SAM-dependent methyltransferase PRMT-type domain. The expression level of AcPRMT1 was highly increased during encystation of A. castellanii. The EGFPAcPRMT1 fusion protein was distributed over the cytoplasm, but it was mainly localized in the nucleus of Acanthamoeba. Knock down of AcPRMT1 by synthetic siRNA with a complementary sequence failed to form mature cysts. These findings suggested that AcPRMT1 plays a critical role in the regulation of encystation of A. castellanii. The target gene of AcPRMT1 regulation and the detailed mechanisms need to be investigated by further studies.

Citations

Citations to this article as recorded by

PRMT5 Inhibitor EPZ015666 Decreases the Viability and Encystment of Entamoeba invadens
Rigoberto Ortiz-Hernández, Elmer Joel Millán-Casarrubias, Jeni Bolaños, Susana Munguía-Robledo, Carlos Vázquez-Calzada, Elisa Azuara-Licéaga, Jesús Valdés, Mario Alberto Rodríguez
Molecules.2024; 30(1): 62. CrossRef
Comparative analysis of differentially expressed genes in Acanthamoeba after ingestion of Legionella pneumophila and Escherichia coli
Eun-Kyung Moon, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
Experimental Parasitology.2022; 232: 108188. CrossRef
Drug Discovery against Acanthamoeba Infections: Present Knowledge and Unmet Needs
Hany M. Elsheikha, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Pathogens.2020; 9(5): 405. CrossRef
Free-living amoebae and squatters in the wild: ecological and molecular features
Ascel Samba-Louaka, Vincent Delafont, Marie-Hélène Rodier, Estelle Cateau, Yann Héchard
FEMS Microbiology Reviews.2019; 43(4): 415. CrossRef

9,989 View
223 Download
4 Web of Science
Crossref

Molecular Characterization of Nippostrongylus brasiliensis (Nematoda: Heligmosomatidae) from Mus musculus in India: Anshu Chaudhary, Urvashi Goswami, Hridaya Shanker Singh; Korean J Parasitol 2016;54(6):743-750.
Published online December 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.6.743

Abstract
PDF

Mus musculus (Rodentia: Muridae) has generally been infected with a rodent hookworm Nippostrongylus brasiliensis. In this report, we present morphological and molecular identification of N. brasiliensis by light and scanning electron microscopy and PCR amplification of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and the protein sequences encoded by cox1 gene, respectively. Despite the use of N. brasiliensis in many biochemistry studies from India, their taxonomic identification was not fully understood, especially at the species level, and no molecular data is available in GenBank from India. Sequence analysis of cox1 gene in this study revealed that the present specimen showed close identity with the same species available in GenBank, confirming that the species is N. brasiliensis. This study represents the first record of molecular identification of N. brasiliensis from India and the protein structure to better understand the comparative phylogenetic characteristics.

Citations

Citations to this article as recorded by

New data on the life cycle of Nippostrongylus brasiliensis (Travassos, 1914) (Nematoda: Heligmosomidae): development of eggs and larval stages in the intestine of naturally infected Rattus norvegicus (Berkenhout, 1769)
María Teresa Galán-Puchades, Mercedes Gómez-Samblás, María Trelis, Antonio Osuna, Rubén Bueno-Marí, Sandra Sáez-Durán, David Bruce Conn, Màrius V. Fuentes
Parasitology Research.2025;[Epub] CrossRef
Severe Infection of Nippostrongylus brasiliensis in Bandicota bengalensis Inhabiting Commensal Areas of Punjab, India: Prevalence, Risk Factor Analysis, Molecular Identification and Phylogenesis
Shivani Rara, Neena Singla, Sukhmanpreet Kaur Brar, Dimple Mandla, Lachhman Das Singla
Acta Parasitologica.2023; 68(1): 172. CrossRef
In Silico dszC Gene Analysis, Modeling and Validation of Dibenzothiophene monooxygenase (DszC Enzyme) of Dibenzothiophene Desulfurizing Streptomyces sp.VUR PPR 102
P. Praveen Reddy, V. UmaMaheswara Rao
Biosciences Biotechnology Research Asia.2023; 20(3): 935. CrossRef
Morphological Re-Description and 18 S rDNA Sequence Confirmation of the Pinworm Aspiculuris tetraptera (Nematoda, Heteroxynematidae) Infecting the Laboratory Mice Mus musculus
Rewaida Abdel-Gaber, Fathy Abdel-Ghaffar, Saleh Al Quraishy, Kareem Morsy, Rehab Saleh, Heinz Mehlhorn
Journal of Nematology.2018; 50(2): 117. CrossRef

11,151 View
170 Download
4 Web of Science
Crossref

Identification of a Novel Microtubule-Binding Protein in Giardia lamblia: Juri Kim, Soon-Jung Park; Korean J Parasitol 2016;54(4):461-469.
Published online August 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.4.461

Abstract
PDF

Giardia lamblia is a protozoan that causes diarrheal diseases in humans. Cytoskeletal structures of Giardia trophozoites must be finely reorganized during cell division. To identify Giardia proteins which interact with microtubules (MTs), Giardia lysates were incubated with in vitro-polymerized MTs and then precipitated by ultracentifugation. A hypothetical protein (GL50803_8405) was identified in the precipitated fraction with polymerized MTs and was named GlMBP1 (G. lamblia microtubule-binding protein 1). Interaction of GlMBP1 with MTs was confirmed by MT binding assays using recombinant GlMBP1 (rGlMBP1). In vivo expression of GlMBP1 was shown by a real-time PCR and western blot analysis using anti-rGlMBP1 antibodies. Transgenic G. lamblia trophozoites were constructed by integrating a chimeric gene encoding hemagglutinin (HA)-tagged GlMBP1 into a Giardia chromosome. Immunofluorescence assays of this transgenic G. lamblia, using anti-HA antibodies, revealed that GlMBP1 mainly localized at the basal bodies, axonemes, and median bodies of G. lamblia trophozoites. This result indicates that GlMBP1 is a component of the G. lamblia cytoskeleton.

Citations

Citations to this article as recorded by

Giardia intraflagellar transport protein 88 is involved in flagella formation
Hye Rim Yeo, Mee Young Shin, Juri Kim, Soon-Jung Park
Parasites, Hosts and Diseases.2025; 63(1): 12. CrossRef
Giardia intestinalis coiled-coil cytolinker protein 259 interacts with actin and tubulin
Omar Rojas-Gutiérrez, Armando Pérez-Rangel, Araceli Castillo-Romero, José Tapia-Ramírez, Emmanuel Ríos-Castro, Minerva Camacho-Nuez, Silvia Giono-Cerezo, Benjamín Nogueda-Torres, Gloria León-Avila, José Manuel Hernández
Parasitology Research.2021; 120(3): 1067. CrossRef

9,627 View
163 Download
3 Web of Science
Crossref

Characterization of Pv92, a Novel Merozoite Surface Protein of Plasmodium vivax: Seong-Kyun Lee, Bo Wang, Jin-Hee Han, Myat Htut Nyunt, Fauzi Muh, Patchanee Chootong, Kwon-Soo Ha, Won Sun Park, Seok-Ho Hong, Jeong-Hyun Park, Eun-Taek Han; Korean J Parasitol 2016;54(4):385-391.
Published online August 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.4.385

Abstract
PDF

The discovery and understanding of antigenic proteins are essential for development of a vaccine against malaria. In Plasmodium falciparum, Pf92 have been characterized as a merozoite surface protein, and this protein is expressed at the late schizont stage, but no study of Pv92, the orthologue of Pf92 in P. vivax, has been reported. Thus, the protein structure of Pv92 was analyzed, and the gene sequence was aligned with that of other Plasmodium spp. using bioinformatics tools. The recombinant Pv92 protein was expressed and purified using bacterial expression system and used for immunization of mice to gain the polyclonal antibody and for evaluation of antigenicity by protein array. Also, the antibody against Pv92 was used for subcellular analysis by immunofluorescence assay. The Pv92 protein has a signal peptide and a sexual stage s48/45 domain, and the cysteine residues at the N-terminal of Pv92 were completely conserved. The N-terminal of Pv92 was successfully expressed as soluble form using a bacterial expression system. The antibody raised against Pv92 recognized the parasites and completely merged with PvMSP1-19, indicating that Pv92 was localized on the merozoite surface. Evaluation of the human humoral immune response to Pv92 indicated moderate antigenicity, with 65% sensitivity and 95% specificity by protein array. Taken together, the merozoite surface localization and antigenicity of Pv92 implicate that it might be involved in attachment and invasion of a merozoite to a new host cell or immune evasion during invasion process.

Citations

Citations to this article as recorded by

Merozoite surface protein 1 paralog is involved in the human erythrocyte invasion of a zoonotic malaria, Plasmodium knowlesi
Seong-Kyun Lee, Tuyet Kha Nguyen, Franziska Mohring, Jin-Hee Han, Egy Rahman Firdaus, Sung-Hun Na, Won-Sun Park, Robert W. Moon, Eun-Taek Han
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
A novel platform for peptide-mediated affinity capture and LC-MS/MS identification of host receptors involved in Plasmodium invasion
Jessica Molina-Franky, David Fernando Plaza, Carmen Merali, Salim Merali, Carlos Barrero, Gabriela Arévalo-Pinzón, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo
Journal of Proteomics.2021; 231: 104002. CrossRef
Inhibition of parasite invasion by monoclonal antibody against epidermal growth factor-like domain of Plasmodium vivax merozoite surface protein 1 paralog
Jin-Hee Han, Yang Cheng, Fauzi Muh, Md Atique Ahmed, Jee-Sun Cho, Myat Htut Nyunt, Hye-Yoon Jeon, Kwon-Soo Ha, Sunghun Na, Won Sun Park, Seok-Ho Hong, Ho-Joon Shin, Bruce Russell, Eun-Taek Han
Scientific Reports.2019;[Epub] CrossRef
Plasmodium vivax in vitro continuous culture: the spoke in the wheel
Maritza Bermúdez, Darwin Andrés Moreno-Pérez, Gabriela Arévalo-Pinzón, Hernando Curtidor, Manuel Alfonso Patarroyo
Malaria Journal.2018;[Epub] CrossRef

10,538 View
257 Download
4 Web of Science
Crossref

Brief Communications

Western Blot Detection of Human Anti-Chikungunya Virus Antibody with Recombinant Envelope 2 Protein: Zhaoshou Yang, Jihoo Lee, Hye-Jin Ahn, Chom-Kyu Chong, Ronaldo F. Dias, Ho-Woo Nam; Korean J Parasitol 2016;54(2):239-241.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.239

Abstract
PDF

Chikungunya virus (CHIKV), a tropical pathogen, has re-emerged and has massive outbreaks abruptly all over the world. Containing many dominant epitopes, the envelope E2 protein of CHIKV has been explored for the vaccination or diagnosis. In the present study, the antigenicity of a recombinant expressed intrinsically disorder domain (IUD) of E2 was tested for the detection of the antibody against CHIKV through western blot method. The gene of the IUD of E2 was inserted into 2 different vectors and expressed as recombinant GST-E2 and recombinant MBP-E2 fusion protein, respectively. Two kinds of fusion proteins were tested with 30 CHIKV patient sera and 30 normal sera, respectively. Both proteins were detected by 25 patients sera (83.3%) and 1 normal serum (3.3%). This test showed a relatively high sensitivity and very high specificity of the recombinant E2 proteins to be used as diagnostic antigens against CHIKV infection.

Citations

Citations to this article as recorded by

Medical viruses: diagnostic techniques
Harsh, Pratima Tripathi
Virology Journal.2023;[Epub] CrossRef
Diagnostic accuracy of serological tests for the diagnosis of Chikungunya virus infection: A systematic review and meta-analysis
Anna Andrew, Tholasi Nadhan Navien, Tzi Shien Yeoh, Marimuthu Citartan, Ernest Mangantig, Magdline S. H. Sum, Ewe Seng Ch’ng, Thean-Hock Tang, Guilherme S. Ribeiro
PLOS Neglected Tropical Diseases.2022; 16(2): e0010152. CrossRef
An Overview of Laboratory Diagnosis of Central Nervous System Viral Infections
Sharifah Aliah Diyanah Syed Hussin, Ang-Lim Chua, Hassanain Al-Talib, Shamala Devi Sekaran, Seok Mui Wang
Journal of Pure and Applied Microbiology.2022; 16(4): 2225. CrossRef
Molecular and Immunological Diagnostic Techniques of Medical Viruses
Daniel Hussien Reta, Tesfaye Sisay Tessema, Addis Simachew Ashenef, Adey Feleke Desta, Wajana Lako Labisso, Solomon Tebeje Gizaw, Solomon Mequanente Abay, Daniel Seifu Melka, Fisseha Alemu Reta
International Journal of Microbiology.2020; 2020: 1. CrossRef
Apigenin enhances the cisplatin cytotoxic effect through p53-modulated apoptosis
Rui Liu, Ping Ji, Bin Liu, Haishi Qiao, Xia Wang, Likun Zhou, Ting Deng, Yi Ba
Oncology Letters.2017; 13(2): 1024. CrossRef

9,200 View
164 Download
6 Web of Science
Crossref

Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae): Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu; Korean J Parasitol 2015;53(6):789-793.
Published online December 31, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.6.789

Abstract
PDF

In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.

Citations

Citations to this article as recorded by

Characterization of tick salivary gland and saliva alphagalactome reveals candidate alpha-gal syndrome disease biomarkers
Margarita Villar, Iván Pacheco, Lourdes Mateos-Hernández, Alejandro Cabezas-Cruz, Ala E. Tabor, Manuel Rodríguez-Valle, Albert Mulenga, Katherine M. Kocan, Edmour F. Blouin, José de La Fuente
Expert Review of Proteomics.2021; 18(12): 1099. CrossRef
Extracellular vesicles induce protective immunity against Trichuris muris
R. K. Shears, A. J. Bancroft, G. W. Hughes, R. K. Grencis, D. J. Thornton
Parasite Immunology.2018;[Epub] CrossRef
New approaches and omics tools for mining of vaccine candidates against vector-borne diseases
Josipa Kuleš, Anita Horvatić, Nicolas Guillemin, Asier Galan, Vladimir Mrljak, Mangesh Bhide
Molecular BioSystems.2016; 12(9): 2680. CrossRef

9,217 View
89 Download
5 Web of Science
Crossref

Original Articles

Potential Interaction of Plasmodium falciparum Hsp60 and Calpain: Seon-Ju Yeo, Dong-Xu Liu, Hyun Park; Korean J Parasitol 2015;53(6):665-673.
Published online December 31, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.6.665

Abstract
PDF

After invasion of red blood cells, malaria matures within the cell by degrading hemoglobin avidly. For enormous protein breakdown in trophozoite stage, many efficient and ordered proteolysis networks have been postulated and exploited. In this study, a potential interaction of a 60-kDa Plasmodium falciparum (Pf)-heat shock protein (Hsp60) and Pf-calpain, a cysteine protease, was explored. Pf-infected RBC was isolated and the endogenous Pf-Hsp60 and Pf-calpain were determined by western blot analysis and similar antigenicity of GroEL and Pf-Hsp60 was determined with anti-Pf-Hsp60. Potential interaction of Pf-calpain and Pf-Hsp60 was determined by immunoprecipitation and immunofluorescence assay. Mizoribine, a well-known inhibitor of Hsp60, attenuated both Pf-calpain enzyme activity as well as P. falciparum growth. The presented data suggest that the Pf-Hsp60 may function on Pf-calpain in a part of networks during malaria growth.

Citations

Citations to this article as recorded by

To explore molecular targets and combination antimalarial chemotherapeutics as tissue and erythrocytic schizonticides
Shilpa Mandal, Nehadrita Chattoraj, Sisir Nandi, Asmita Samadder
Journal of Parasitic Diseases.2025;[Epub] CrossRef
Controlling drug resistance by targeting Plasmodium falciparum heat shock protein 70-1, a chaperone at the centre of protein quality control mechanism: a review
Douglas A. M. Ruhwaya, Brilliant Nyathi, Gadzikano Munyuki, Ryman Shoko, Grace Mugumbate
All Life.2023;[Epub] CrossRef
Development of a Rapid Fluorescent Diagnostic System to Detect Subtype H9 Influenza A Virus in Chicken Feces
Hien Thi Tuong, Ju Hwan Jeong, Young Ki Choi, Hyun Park, Yun Hee Baek, Seon-Ju Yeo
International Journal of Molecular Sciences.2021; 22(16): 8823. CrossRef
Small Molecule Inhibitors Targeting the Heat Shock Protein System of Human Obligate Protozoan Parasites
Tawanda Zininga, Addmore Shonhai
International Journal of Molecular Sciences.2019; 20(23): 5930. CrossRef
Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus
Trinh Thi Thuy Tien, Hyun Park, Hien Thi Tuong, Seung-Taek Yu, Du-Young Choi, Seon-Ju Yeo
International Journal of Molecular Sciences.2018; 19(10): 3013. CrossRef
Rapid detection of avian influenza A virus by immunochromatographic test using a novel fluorescent dye
Seon-Ju Yeo, Bui Thi Cuc, Soon-Ai Kim, Do Thi Hoang Kim, Duong Tuan Bao, Trinh Thi Thuy Tien, Nguyen Thi Viet Anh, Do-Young Choi, Chom-Kyu Chong, Hak Sung Kim, Hyun Park
Biosensors and Bioelectronics.2017; 94: 677. CrossRef
Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles
Seon-Ju Yeo, Duong Tuan Bao, Ga-Eun Seo, Cuc Thi Bui, Do Thi Hoang Kim, Nguyen Thi Viet Anh, Trinh Thi Thuy Tien, Nguyen Thi Phuong Linh, Hae-Jin Sohn, Chom-Kyu Chong, Ho-Joon Shin, Hyun Park
Scientific Reports.2017;[Epub] CrossRef

10,540 View
110 Download
8 Web of Science
Crossref

Identification of Immunodominant B-cell Epitope Regions of Reticulocyte Binding Proteins in Plasmodium vivax by Protein Microarray Based Immunoscreening: Jin-Hee Han, Jian Li, Bo Wang, Seong-Kyun Lee, Myat Htut Nyunt, Sunghun Na, Jeong-Hyun Park, Eun-Taek Han; Korean J Parasitol 2015;53(4):403-411.
Published online August 25, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.4.403

Abstract
PDF

Plasmodium falciparum can invade all stages of red blood cells, while Plasmodium vivax can invade only reticulocytes. Although many P. vivax proteins have been discovered, their functions are largely unknown. Among them, P. vivax reticulocyte binding proteins (PvRBP1 and PvRBP2) recognize and bind to reticulocytes. Both proteins possess a C-terminal hydrophobic transmembrane domain, which drives adhesion to reticulocytes. PvRBP1 and PvRBP2 are large (> 326 kDa), which hinders identification of the functional domains. In this study, the complete genome information of the P. vivax RBP family was thoroughly analyzed using a prediction server with bioinformatics data to predict B-cell epitope domains. Eleven pvrbp family genes that included 2 pseudogenes and 9 full or partial length genes were selected and used to express recombinant proteins in a wheat germ cell-free system. The expressed proteins were used to evaluate the humoral immune response with vivax malaria patients and healthy individual serum samples by protein microarray. The recombinant fragments of 9 PvRBP proteins were successfully expressed; the soluble proteins ranged in molecular weight from 16 to 34 kDa. Evaluation of the humoral immune response to each recombinant PvRBP protein indicated a high antigenicity, with 38-88% sensitivity and 100% specificity. Of them, N-terminal parts of PvRBP2c (PVX_090325-1) and PvRBP2 like partial A (PVX_090330-1) elicited high antigenicity. In addition, the PvRBP2-like homologue B (PVX_116930) fragment was newly identified as high antigenicity and may be exploited as a potential antigenic candidate among the PvRBP family. The functional activity of the PvRBP family on merozoite invasion remains unknown.

Citations

Citations to this article as recorded by

Alternative Invasion Mechanisms and Host Immune Response to Plasmodium vivax Malaria: Trends and Future Directions
Daniel Kepple, Kareen Pestana, Junya Tomida, Abnet Abebe, Lemu Golassa, Eugenia Lo
Microorganisms.2020; 9(1): 15. CrossRef
Epitope-Based Vaccine Designing of Nocardia asteroides Targeting the Virulence Factor Mce-Family Protein by Immunoinformatics Approach
Prasanta Patra, Niladri Mondal, Bidhan Chandra Patra, Manojit Bhattacharya
International Journal of Peptide Research and Therapeutics.2020; 26(2): 1165. CrossRef
Plasmodium vivax Reticulocyte Binding Proteins for invasion into reticulocytes
Li‐Jin Chan, Melanie H. Dietrich, Wang Nguitragool, Wai‐Hong Tham
Cellular Microbiology.2020;[Epub] CrossRef
From a basic to a functional approach for developing a blood stage vaccine against Plasmodium vivax
Manuel Alfonso Patarroyo, Gabriela Arévalo-Pinzón, Darwin A. Moreno-Pérez
Expert Review of Vaccines.2020; 19(2): 195. CrossRef
Inferring Plasmodium vivax protein biology by using omics data
D.A. Moreno-Pérez, M.A. Patarroyo
Journal of Proteomics.2020; 218: 103719. CrossRef
Prediction of B cell and T‐helper cell epitopes candidates of bovine leukaemia virus (BLV) by in silico approach
Negar Hooshmand, Jamal Fayazi, Saleh Tabatabaei, Nader Ghaleh Golab Behbahan
Veterinary Medicine and Science.2020; 6(4): 730. CrossRef
Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
Pyo Yun Cho, Ji-Yun Lee, Tae Im Kim, Jin-Ho Song, Sung-Jong Hong, Won Gi Yoo, Takafumi Tsuboi, Kwon-Soo Ha, Jae-Wan Jung, Satoru Takeo, Eun-Taek Han, Banchob Sripa, Sung-Tae Hong, Jong-Yil Chai, Ho-Woo Nam, Jhang Ho Pak, Tong-Soo Kim, Krystyna Cwiklinski
PLOS Neglected Tropical Diseases.2020; 14(12): e0008998. CrossRef
Contribution ofPlasmodiumimmunomics: potential impact for serological testing and surveillance of malaria
Kokouvi Kassegne, Eniola Michael Abe, Yan-Bing Cui, Shen-Bo Chen, Bin Xu, Wang-Ping Deng, Hai-Mo Shen, Yue Wang, Jun-Hu Chen, Xiao-Nong Zhou
Expert Review of Proteomics.2019; 16(2): 117. CrossRef
Identification and Immunological Characterization of the Ligand Domain of Plasmodium vivax Reticulocyte Binding Protein 1a
Francis B Ntumngia, Richard Thomson-Luque, Sandra Galusic, Gabriel Frato, Sarah Frischmann, David S Peabody, Bryce Chackerian, Marcelo U Ferreira, Christopher L King, John H Adams
The Journal of Infectious Diseases.2018; 218(7): 1110. CrossRef
Plasmodium vivax vaccine research – we’ve only just begun
Wai-Hong Tham, James G. Beeson, Julian C. Rayner
International Journal for Parasitology.2017; 47(2-3): 111. CrossRef
What Is Known about the Immune Response Induced by Plasmodium vivax Malaria Vaccine Candidates?
Carolina López, Yoelis Yepes-Pérez, Natalia Hincapié-Escobar, Diana Díaz-Arévalo, Manuel A. Patarroyo
Frontiers in Immunology.2017;[Epub] CrossRef
Identification of a reticulocyte-specific binding domain of Plasmodium vivax reticulocyte-binding protein 1 that is homologous to the PfRh4 erythrocyte-binding domain
Jin-Hee Han, Seong-Kyun Lee, Bo Wang, Fauzi Muh, Myat Htut Nyunt, Sunghun Na, Kwon-Soo Ha, Seok-Ho Hong, Won Sun Park, Jetsumon Sattabongkot, Takafumi Tsuboi, Eun-Taek Han
Scientific Reports.2016;[Epub] CrossRef
Plasmodium vivax GPI-anchored micronemal antigen (PvGAMA) binds human erythrocytes independent of Duffy antigen status
Yang Cheng, Feng Lu, Bo Wang, Jian Li, Jin-Hee Han, Daisuke Ito, Deok-Hoon Kong, Lubin Jiang, Jian Wu, Kwon-Soo Ha, Eizo Takashima, Jetsumon Sattabongkot, Jun Cao, Myat Htut Nyunt, Myat Phone Kyaw, Sanjay A. Desai, Louis H. Miller, Takafumi Tsuboi, Eun-Ta
Scientific Reports.2016;[Epub] CrossRef
Plasmodium vivax Reticulocyte Binding Proteins Are Key Targets of Naturally Acquired Immunity in Young Papua New Guinean Children
Camila T. França, Wen-Qiang He, Jakub Gruszczyk, Nicholas T. Y. Lim, Enmoore Lin, Benson Kiniboro, Peter M. Siba, Wai-Hong Tham, Ivo Mueller, Henk D. F. H. Schallig
PLOS Neglected Tropical Diseases.2016; 10(9): e0005014. CrossRef
Gene Models, Expression Repertoire, and Immune Response of Plasmodium vivax Reticulocyte Binding Proteins
Jenni Hietanen, Anongruk Chim-ong, Thanprakorn Chiramanewong, Jakub Gruszczyk, Wanlapa Roobsoong, Wai-Hong Tham, Jetsumon Sattabongkot, Wang Nguitragool, J. H. Adams
Infection and Immunity.2016; 84(3): 677. CrossRef

12,028 View
153 Download
16 Web of Science
Crossref

Brief Communication

Cloning and Iron Transportation of Nucleotide Binding Domain of Cryptosporidium andersoni ATP-Binding Cassette (CaABC) Gene: Ju-Hua Wang, Xiu-Heng Xue, Jie Zhou, Cai-Yun Fan, Qian-Qian Xie, Pan Wang; Korean J Parasitol 2015;53(3):335-339.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.335

Abstract
PDF

Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of Ca²⁺, Mg²⁺, K⁺, and HCO₃^- in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.

8,458 View
77 Download

Original Articles

Allelic Diversity and Geographical Distribution of the Gene Encoding Plasmodium falciparum Merozoite Surface Protein-3 in Thailand: Vorthon Sawaswong, Phumin Simpalipan, Napaporn Siripoon, Pongchai Harnyuttanakorn, Sittiporn Pattaradilokrat; Korean J Parasitol 2015;53(2):177-187.
Published online April 22, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.2.177

Abstract
PDF

Merozoite surface proteins (MSPs) of malaria parasites play critical roles during the erythrocyte invasion and so are potential candidates for malaria vaccine development. However, because MSPs are often under strong immune selection, they can exhibit extensive genetic diversity. The gene encoding the merozoite surface protein-3 (MSP-3) of Plasmodium falciparum displays 2 allelic types, K1 and 3D7. In Thailand, the allelic frequency of the P. falciparum msp-3 gene was evaluated in a single P. falciparum population in Tak at the Thailand and Myanmar border. However, no study has yet looked at the extent of genetic diversity of the msp-3 gene in P. falciparum populations in other localities. Here, we genotyped the msp-3 alleles of 63 P. falciparum samples collected from 5 geographical populations along the borders of Thailand with 3 neighboring countries (Myanmar, Laos, and Cambodia). Our study indicated that the K1 and 3D7 alleles coexisted, but at different proportions in different Thai P. falciparum populations. K1 was more prevalent in populations at the Thailand-Myanmar and Thailand-Cambodia borders, whilst 3D7 was more prevalent at the Thailand-Laos border. Global analysis of the msp-3 allele frequencies revealed that proportions of K1 and 3D7 alleles of msp-3 also varied in different continents, suggesting the divergence of malaria parasite populations. In conclusion, the variation in the msp-3 allelic patterns of P. falciparum in Thailand provides fundamental knowledge for inferring the P. falciparum population structure and for the best design of msp-3 based malaria vaccines.

Citations

Citations to this article as recorded by

Genetic diversity of Plasmodium falciparum AMA-1 antigen from the Northeast Indian state of Tripura and comparison with global sequences: implications for vaccine development
Tulika Nirmolia, Md. Atique Ahmed, Vinayagam Sathishkumar, Nilanju P. Sarma, Dibya R. Bhattacharyya, Pradyumna K. Mohapatra, Devendra Bansal, Praveen K. Bharti, Rakesh Sehgal, Jagadish Mahanta, Ali A. Sultan, Kanwar Narain, Saurav J. Patgiri
Malaria Journal.2022;[Epub] CrossRef
Genetic polymorphism of merozoite surface protein-3 in Myanmar Plasmodium falciparum field isolates
Hương Giang Lê, Thị Lam Thái, Jung-Mi Kang, Jinyoung Lee, Mya Moe, Tuấn Cường Võ, Haung Naw, Moe Kyaw Myint, Zaw Than Htun, Tong-Soo Kim, Ho-Joon Shin, Byoung-Kuk Na
Malaria Journal.2020;[Epub] CrossRef
Unraveling Haplotype Diversity of the Apical Membrane Antigen-1 Gene in Plasmodium falciparum Populations in Thailand
Lalita Lumkul, Vorthon Sawaswong, Phumin Simpalipan, Morakot Kaewthamasorn, Pongchai Harnyuttanakorn, Sittiporn Pattaradilokrat
The Korean Journal of Parasitology.2018; 56(2): 153. CrossRef
Genetic diversity of the merozoite surface protein-3 gene in Plasmodium falciparum populations in Thailand
Sittiporn Pattaradilokrat, Vorthon Sawaswong, Phumin Simpalipan, Morakot Kaewthamasorn, Napaporn Siripoon, Pongchai Harnyuttanakorn
Malaria Journal.2016;[Epub] CrossRef

11,977 View
114 Download
4 Web of Science
Crossref

Relationship between Antibody-Positive Rate against Plasmodium vivax Circumsporozoite Protein and Incidence of Malaria: Hyeong-Woo Lee, Yoon-Joong Kang, Shin-Hyeong Cho, Byoung-Kuk Na, Jhang Ho Pak, Ho-Woo Nam, Yun-Kyu Park, Youngjoo Sohn, Tong-Soo Kim; Korean J Parasitol 2015;53(2):169-175.
Published online April 22, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.2.169

Abstract
PDF

The relationship between anti-Plasmodium vivax circumsporozoite protein (CSP) antibody levels and the prevalence of malaria in epidemic areas of South Korea was evaluated. Blood samples were collected from inhabitants of Gimpo-si (city), Paju-si, and Yeoncheon-gun (county) in Gyeonggi-do (province), as well as Cheorwon-gun in Gangwon-do from November to December 2004. Microscopic examinations were used to identify malaria parasites. ELISA was used to quantitate anti-circumsporozoite protein (CSP) antibodies against P. vivax. A total of 1,774 blood samples were collected. The overall CSP-ELISA-positive rate was 7.7% (n=139). The annual parasite incidences (APIs) in these areas gradually decreased from 2004 to 2005 (1.09 and 0.80, respectively). The positive rate in Gimpo (10.4%, 44/425) was the highest identified by CSP-ELISA. The highest API was found in Yeoncheon, followed by Cheorwon, Paju, and Gimpo in both years. The positive rates of CSP-ELISA were closely related to the APIs in the study areas. These results suggest that seroepidemiological studies based on CSP may be helpful in estimating the malaria prevalence in certain areas. In addition, this assay can be used to establish and evaluate malaria control and eradication programs in affected areas.

Citations

Citations to this article as recorded by

Updated global distribution of Plasmodium vivax circumsporozoite protein variants and their correlation with vector susceptibility: A systematic review and meta-analysis
Marrara P. Sampaio, Marcelo Cerilo-Filho, Yasmin de A․G․do Amaral, Maria Naely G. Almeida, Rayanne I. Correa, Dulce J.V. Fernando, Nathália F. Reis, Leonardo A. Miceli, José R.S. Silva, Luciane M. Storti-Melo, Andréa R.S. Baptista, Ricardo L.D. Machado
Acta Tropica.2025; 261: 107508. CrossRef
Using Serological Markers for the Surveillance of Plasmodium vivax Malaria: A Scoping Review
Lejla Kartal, Ivo Mueller, Rhea J. Longley
Pathogens.2023; 12(6): 791. CrossRef

9,658 View
124 Download
2 Web of Science
Crossref

Proteomic Screening of Antigenic Proteins from the Hard Tick, Haemaphysalis longicornis (Acari: Ixodidae): Young-Ha Kim, Mohammad Saiful slam, Myung-Jo You; Korean J Parasitol 2015;53(1):85-93.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.85

Abstract
PDF

Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional electrophoresis (2-DE) immunoblotting was used for whole body proteins from unfed and partially fed female ticks. Reactivity bands and 2-DE immunoblotting were performed following 2-DE electrophoresis to identify protein spots. The proteome of the partially fed female had a larger number of lower molecular weight proteins than that of the unfed female tick. The total number of detected spots was 818 for unfed and 670 for partially fed female ticks. The 2-DE immunoblotting identified 10 antigenic spots from unfed females and 8 antigenic spots from partially fed females. Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF) of relevant spots identified calreticulin, putative secreted WC salivary protein, and a conserved hypothetical protein from the National Center for Biotechnology Information and Swiss Prot protein sequence databases. These findings indicate that most of the whole body components of these ticks are non-immunogenic. The data reported here will provide guidance in the identification of antigenic proteins to prevent infestation and diseases transmitted by H. longicornis.

Citations

Citations to this article as recorded by

Developmental Proteomics Reveals the Dynamic Expression Profile of Global Proteins of Haemaphysalis longicornis (Parthenogenesis)
Min-Xuan Liu, Xiao-Pei Xu, Fan-Ming Meng, Bing Zhang, Wei-Gang Li, Yuan-Yuan Zhang, Qiao-Ying Zen, Wen-Ge Liu
Life.2025; 15(1): 59. CrossRef
Haemaphysalis longicornis calreticulin is not an effective molecular tool for tick bite diagnosis and disruption of tick infestations
Weiqing Zheng, Haijun Hu, Jiafu Jiang, Xiangrong Sun, Renlong Fu, Huiying Tao, Yangqing Liu, Haiying Chen, Hongmei Ma, Shengen Chen
Veterinary Parasitology.2022; 309: 109775. CrossRef
Trypanosoma cruzi Calreticulin: Immune Evasion, Infectivity, and Tumorigenesis
Galia Ramírez-Toloza, Eduardo Sosoniuk-Roche, Carolina Valck, Lorena Aguilar-Guzmán, Viviana P. Ferreira, Arturo Ferreira
Trends in Parasitology.2020; 36(4): 368. CrossRef
Catalogue of stage-specific transcripts in Ixodes ricinus and their potential functions during the tick life-cycle
Pavlina Vechtova, Zoltan Fussy, Radim Cegan, Jan Sterba, Jan Erhart, Vladimir Benes, Libor Grubhoffer
Parasites & Vectors.2020;[Epub] CrossRef
Comparative Tandem Mass Tag-Based Quantitative Proteomic Analysis of Tachaea chinensis Isopod During Parasitism
Yingdong Li, Xin Li, Zhibin Han, Weibin Xu, Xiaodong Li, Qijun Chen
Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
Applying proteomics to tick vaccine development: where are we?
Margarita Villar, Anabel Marina, José de la Fuente
Expert Review of Proteomics.2017; 14(3): 211. CrossRef
Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae)
Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu
The Korean Journal of Parasitology.2015; 53(6): 789. CrossRef

10,715 View
108 Download
7 Web of Science
Crossref

Immunopathological Changes in the Brain of Immunosuppressed Mice Experimentally Infected with Toxocara canis: Mohamed M. Eid, Samy I. El-Kowrany, Ahmad A. Othman, Dina I. El Gendy, Eman M. Saied; Korean J Parasitol 2015;53(1):51-58.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.51

Abstract
PDF

Toxocariasis is a soil-transmitted helminthozoonosis due to infection of humans by larvae of Toxocara canis. The disease could produce cognitive and behavioral disturbances especially in children. Meanwhile, in our modern era, the incidence of immunosuppression has been progressively increasing due to increased incidence of malignancy as well as increased use of immunosuppressive agents. The present study aimed at comparing some of the pathological and immunological alterations in the brain of normal and immunosuppressed mice experimentally infected with T. canis. Therefore, 180 Swiss albino mice were divided into 4 groups including normal (control) group, immunocompetent T. canis-infected group, immunosuppressed group (control), and immunosuppressed infected group. Infected mice were subjected to larval counts in the brain, and the brains from all mice were assessed for histopathological changes, astrogliosis, and IL-5 mRNA expression levels in brain tissues. The results showed that under immunosuppression, there were significant increase in brain larval counts, significant enhancement of reactive gliosis, and significant reduction in IL-5 mRNA expression. All these changes were maximal in the chronic stage of infection. In conclusion, the immunopathological alterations in the brains of infected animals were progressive over time, and were exaggerated under the effect of immunosuppression as did the intensity of cerebral infection.

Citations

Citations to this article as recorded by

Toxocara canis infections in mice: from subtle to severe consequences in 100 weeks
Nicol Bernardová, Jan Novák, Chia-Kwung Fan, Libuše Kolářová, Marta Chanová
Journal of Helminthology.2025;[Epub] CrossRef
Using Biocontrol Fungi to Control Helminthosis in Wild Animals: An Innovative Proposal for the Health and Conservation of Species
Júlia dos Santos Fonseca, Beatriz Bacelar Barbosa, Adolfo Paz Silva, María Sol Arias Vázquez, Cristiana Filipa Cazapal Monteiro, Huarrisson Azevedo Santos, Jackson Victor de Araújo
Pathogens.2025; 14(8): 775. CrossRef
The potential relationship between Toxocara canis infection and epilepsy in a rat model
Amina M. Salama, Dina I. Elgendy, Rasha A. Elmahy, Asmaa F. Eltantawy, Monira A. Seleem, Ahmed M. Elgohary, Omaima K. Docmac, Marwa E. F. Al Mwafy, Ahmed A. Almeldin, Mahmoud S. Sharaf
Parasitology Research.2025;[Epub] CrossRef
Efficacy of artemether against toxocariasis in mice: parasitological and immunopathological changes in brain, liver, and lung
Dina I. Elgendy, Rasha A. Elmahy, Alaa Ibrahim Mohamed Amer, Hoda A. Ibrahim, Asmaa Fawzy Eltantawy, Fotouh Rashed Mansour, Amina M. Salama
Pathogens and Global Health.2024; 118(1): 47. CrossRef
Neurobehavioral Disorders and Pathological Changes in the Brain of Mice Are Caused by Chronic Toxocara canis Larval Invasion with Low to Moderate Inoculum
Nicol Bernardová, Jan Novák, Petr Horák, Chia-Kwung Fan, Libuše Kolářová
Acta Parasitologica.2024; 69(4): 1736. CrossRef
Effects of metformin on parasitological, pathological changes in the brain and liver and immunological aspects during visceral toxocariasis in mice
Amina M. Salama, Rasha A. Elmahy, Hoda A. Ibrahim, Alaa Ibrahim Mohamed Amer, Asmaa Fawzy Eltantawy, Dina I. Elgendy
Parasitology Research.2023; 122(12): 3213. CrossRef
Cyclosporine A increases the intensity of Toxocara canis infection in swiss mice
W. D. S. Terto, M. Q. de Moura, J. L. Borchardt, F. D. S. Santos, L. F. da Costa Avila, N. B. Pinheiro, F. P. Leivas Leite, M. M. Villela, M. E. A. Berne
Brazilian Journal of Biology.2022;[Epub] CrossRef
Glial Fibrillary Acidic Protein and Ionized Calcium-Binding Adapter Molecule 1 Immunostaining Score for the Central Nervous System of Horses With Non-suppurative Encephalitis and Encephalopathies
Gisele Silva Boos, Klaus Failing, Edson Moleta Colodel, David Driemeier, Márcio Botelho de Castro, Daniele Mariath Bassuino, José Diomedes Barbosa, Christiane Herden
Frontiers in Veterinary Science.2021;[Epub] CrossRef
Diallyl sulfide alleviates cyclophosphamide-induced nephropathic encephalopathy in rats
Shereen M. Galal, Heba H. Mansour, Abeer A. Elkhoely
Toxicology Mechanisms and Methods.2020; 30(3): 208. CrossRef
Mesenchymal stem cells combined with albendazole as a novel therapeutic approach for experimental neurotoxocariasis
E. V. N. Beshay, S. A. El-Refai, G. S. Sadek, A. A. Elbadry, F. H. Shalan, A. F. Afifi
Parasitology.2020; 147(7): 799. CrossRef
Histopathological characterization of Toxocara canis- and T. cati-induced neurotoxocarosis in the mouse model
Andrea Springer, Lea Heuer, Elisabeth Janecek-Erfurth, Andreas Beineke, Christina Strube
Parasitology Research.2019; 118(9): 2591. CrossRef
Developmental stages and viability of Toxocara canis eggs outside the host
Iman Fathy Abou-El-Naga
Biomédica.2018; 38(2): 189. CrossRef
Seroprevalence and Risk Factors for Toxocara canis Infection in Serbia During 2015
Simona Gabrielli, Suzana Tasić-Otašević, Aleksandra Ignjatović, Maurizio Fraulo, Marija Trenkić-Božinović, Stefan Momčilović, Gabriella Cancrini
Foodborne Pathogens and Disease.2017; 14(1): 43. CrossRef

12,707 View
160 Download
16 Web of Science
Crossref

Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells: Juan-Hua Quan, In-Wook Choi, Jung-Bo Yang, Wei Zhou, Guang-Ho Cha, Yu Zhou, Jae-Sook Ryu, Young-Ha Lee; Korean J Parasitol 2014;52(6):595-603.
Published online December 23, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.6.595

Abstract
PDF

Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite.

Citations

Citations to this article as recorded by

Chromatin accessibility and gene expression in the parasite Trichomonas vaginalis
Agustina Prat, Daniela Muñoz, Ayelen Lizarraga, Julieta Seifert-Gorzycki, Estefania Sanchez-Vazquez, Patricia J. Johnson, Pablo H. Strobl-Mazzulla, Natalia de Miguel
BMC Infectious Diseases.2025;[Epub] CrossRef
Metallopeptidases as Key Virulence Attributes of Clinically Relevant Protozoa: New Discoveries, Perspectives, and Frontiers of Knowledge
Graziela Vargas Rigo, Fernanda Gomes Cardoso, Giulia Bongiorni Galego, Deisiane Fernanda da Rosa, André Luis Souza dos Santos, Tiana Tasca
Current Protein & Peptide Science.2023; 24(4): 307. CrossRef
VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles
Nehuén Salas, Veronica M. Coceres, Tuanne dos Santos Melo, Antonio Pereira-Neves, Vanina G. Maguire, Tania M. Rodriguez, Bruna Sabatke, Marcel I. Ramirez, Jihui Sha, James A. Wohlschlegel, Natalia de Miguel
Cellular and Molecular Life Sciences.2022;[Epub] CrossRef
Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated Lactobacillus gasseri Confer Non-Identical Cell Protection against Trichomonas vaginalis-Induced Cell Detachment
Bénédicte Pradines, Séverine Domenichini, Vanessa Lievin-Le Moal
Pharmaceuticals.2022; 15(11): 1350. CrossRef
The 50 kDa metalloproteinase TvMP50 is a zinc-mediated Trichomonas vaginalis virulence factor
Jonathan Puente-Rivera, José Luis Villalpando, Alma Villalobos-Osnaya, Laura Isabel Vázquez-Carrillo, Gloria León-Ávila, María Dolores Ponce-Regalado, César López-Camarillo, Jose Miguel Elizalde-Contreras, Eliel Ruiz-May, Rossana Arroyo, María Elizbeth Al
Molecular and Biochemical Parasitology.2017; 217: 32. CrossRef
Regulation of exosomes released from normal ovarian epithelial cells and ovarian cancer cells
Wei Zhang, Jiaxin Yang, Dongyan Cao, Yan You, Keng Shen, Peng Peng
Tumor Biology.2016; 37(12): 15763. CrossRef
Involvement of PI3K/AKT and MAPK Pathways for TNF-α Production in SiHa Cervical Mucosal Epithelial Cells Infected with <i>Trichomonas vaginalis</i>
Jung-Bo Yang, Juan-Hua Quan, Ye-Eun Kim, Yun-Ee Rhee, Byung-Hyun Kang, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee
The Korean Journal of Parasitology.2015; 53(4): 371. CrossRef

11,452 View
103 Download
7 Web of Science
Crossref

Amoebic PI3K and PKC Is Required for Jurkat T Cell Death Induced by Entamoeba histolytica: Young Ah Lee, Kyeong Ah Kim, Arim Min, Myeong Heon Shin; Korean J Parasitol 2014;52(4):355-365.
Published online August 29, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.4.355

Abstract
PDF

The enteric protozoan parasite Entamoeba histolytica is the causative agent of human amebiasis. During infection, adherence of E. histolytica through Gal/GalNAc lectin on the surface of the amoeba can induce caspase-3-dependent or -independent host cell death. Phosphorylinositol 3-kinase (PI3K) and protein kinase C (PKC) in E. histolytica play an important function in the adhesion, killing, or phagocytosis of target cells. In this study, we examined the role of amoebic PI3K and PKC in amoeba-induced apoptotic cell death in Jurkat T cells. When Jurkat T cells were incubated with E. histolytica trophozoites, phosphatidylserine (PS) externalization and DNA fragmentation in Jurkat cells were markedly increased compared to those of cells incubated with medium alone. However, when amoebae were pretreated with a PI3K inhibitor, wortmannin before being incubated with E. histolytica, E. histolytica-induced PS externalization and DNA fragmentation in Jurkat cells were significantly reduced compared to results for amoebae pretreated with DMSO. In addition, pretreatment of amoebae with a PKC inhibitor, staurosporine strongly inhibited Jurkat T cell death. However, E. histolytica-induced cleavage of caspase-3, -6, and -7 were not inhibited by pretreatment of amoebae with wortmannin or staurosporin. In addition, we found that amoebic PI3K and PKC have an important role on amoeba adhesion to host compartment. These results suggest that amebic PI3K and PKC activation may play an important role in caspase-independent cell death in Entamoeba-induced apoptosis.

Citations

Citations to this article as recorded by

PI3K/AKT signaling in parasites and parasite diseases: Role and therapeutic potential
Lujun Yan, Yating Li, Xing Yang, Rui Li, Chunyin Zhu, Xuedong He, Xiaoliang Jin, Guanghui Zheng, Naunain Mehmood, William C. Cho, Shijun Bao, Houhui Song, Yadong Zheng
Virulence.2025;[Epub] CrossRef
Formation and culture of cell spheroids by using magnetic nanostructures resembling a crown of thorns
Shijiao Li, Jingjiang Qiu, Zhongwei Guo, Qiulei Gao, Chen-Yu Huang, Yilin Hao, Yifan Hu, Tianshui Liang, Ming Zhai, Yudong Zhang, Bangbang Nie, Wei-Jen Chang, Wen Wang, Rui Xi, Ronghan Wei
Biofabrication.2024; 16(4): 045018. CrossRef
AGC family kinase of Entamoeba histolytica: Decoding the members biochemically
Azhar Ahmad, Vikas Kumar, Tushar Kushwaha, Akash Kumar, Deepak Sehgal, Krishna K. Inampudi, Somlata, Katherine S. Ralston
PLOS Pathogens.2024; 20(11): e1012729. CrossRef
Pathogenicity and virulence of Entamoeba histolytica , the agent of amoebiasis
Nancy Guillén
Virulence.2023;[Epub] CrossRef
Apoptotic mimicry as a strategy for the establishment of parasitic infections: parasite- and host-derived phosphatidylserine as key molecule
João Luiz Mendes Wanderley, Renato Augusto DaMatta, Marcello André Barcinski
Cell Communication and Signaling.2020;[Epub] CrossRef
Entamoeba histolytica Up-Regulates MicroRNA-643 to Promote Apoptosis by Targeting XIAP in Human Epithelial Colon Cells
Itzel López-Rosas, César López-Camarillo, Yarely M. Salinas-Vera, Olga N. Hernández-de la Cruz, Carlos Palma-Flores, Bibiana Chávez-Munguía, Osbaldo Resendis-Antonio, Nancy Guillen, Carlos Pérez-Plasencia, María Elizbeth Álvarez-Sánchez, Esther Ramírez-Mo
Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
Phosphatidylinositol Kinases and Phosphatases in Entamoeba histolytica
Kumiko Nakada-Tsukui, Natsuki Watanabe, Tomohiko Maehama, Tomoyoshi Nozaki
Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
AGC family kinase 1 participates in trogocytosis but not in phagocytosis in Entamoeba histolytica
Somlata, Kumiko Nakada-Tsukui, Tomoyoshi Nozaki
Nature Communications.2017;[Epub] CrossRef

10,208 View
87 Download
9 Web of Science
Crossref

Brief Communication

Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes: Eun-Taek Han, Hyun-Jong Yang, Young-Jin Park, Jeong-Hyun Park, Jong-Yil Chai; Korean J Parasitol 2014;52(2):201-204.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.201

Abstract
PDF

This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species.

Citations

Citations to this article as recorded by

Reliability of heterophyid antigens in heterologous protection against human schistosomiasis
Alaa H. A. Hegazy, Lamia A. Galal, Tasneem M. Hassan, Refaat M. A. Khalifa
Journal of Parasitic Diseases.2020; 44(2): 349. CrossRef
Fishborne zoonotic heterophyid infections: An update
Jong-Yil Chai, Bong-Kwang Jung
Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef

8,993 View
84 Download
3 Web of Science
Crossref

Original Articles

Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients: Ho-Woo Nam, Kyoung Ju Song, Hye Jin Ahn, Zhaoshou Yang, Chom-Kyu Chong, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim; Korean J Parasitol 2014;52(2):143-149.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.143

Abstract
PDF

To evaluate the seroprevalence against circumsporozoite protein (CSP) of Plasmodium vivax in sera of Korean patients, the central repeating domain (CRD) of CSP was cloned and analyzed. From the genomic DNA of patient's blood, 2 kinds of CSPs were identified to belong to a VK210 type, which is the dominant repeating of GDRA(D/A)GQPA, and named as PvCSPA and PvCSPB. Recombinantly expressed his-tagged PvCSPA or PvCSPB in Escherichia coli reacted well against sera of patients in western blot, with the detecting rate of 47.9% (58/121), which included 15 cases positive for PvCSPA, 6 cases positive for PvCSPB, and 37 cases for both. The mixture of PvCSPA and PvCSPB was loaded to a rapid diagnostic test kit (RDT) and applied with the same set of patient sera, which resulted in detection rates of 57.0% (69/121). When the protein sequences of PvCSPA were compared with those of P. vivax in endemic regions of India and Uganda, they were compatibly homologous to PvCSPA with minor mutations. These results suggested that the recombinant PvCSPA and PvCSPB loaded RDT may be a milestone in latent diagnosis which has been a hot issue of domestic malaria and important for radical therapy in overlapped infections with P. falciparum in tropical and subtropical areas. During the biological process of malarial infection, exposure of CSP to antigen-antibody reaction up to 57.0% is the first report in Korea.

Citations

Citations to this article as recorded by

Using Serological Markers for the Surveillance of Plasmodium vivax Malaria: A Scoping Review
Lejla Kartal, Ivo Mueller, Rhea J. Longley
Pathogens.2023; 12(6): 791. CrossRef
A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery
Seda Yerlikaya, Ewurama D A Owusu, Augustina Frimpong, Robert Kirk DeLisle, Xavier C Ding
Clinical Infectious Diseases.2022; 74(1): 40. CrossRef
Structure-genetic diversity and recombinant protein of circumsporozoite protein (CSP) of vivax malaria antigen: A potential malaria vaccine candidate
Vahid Raissi, Soudabeh Etemadi, Muhammad Ibrahim Getso, Ahmad Mehravaran, Omid Raiesi
Gene Reports.2021; 23: 101132. CrossRef
Serological responses to a soluble recombinant circumsporozoite protein-VK210 of Plasmodium vivax (rPvCSP-VK210) among Iranian malaria patients
Mehdi Nateghpour, Soudabeh Etemadi, Afsaneh Motevalli Haghi, Hamid Eslami, Mehdi Mohebali, Leila Farivar
European Journal of Medical Research.2021;[Epub] CrossRef
Recent Advances in the Development of Biosensors for Malaria Diagnosis
Francis D. Krampa, Yaw Aniweh, Prosper Kanyong, Gordon A. Awandare
Sensors.2020; 20(3): 799. CrossRef
Genetic Diversity of Plasmodium vivax Causing Epidemic Malaria in the Republic of Korea
Young Yil Bahk, Jeonga Kim, Seong Kyu Ahn, Byoung-Kuk Na, Jong-Yil Chai, Tong-Soo Kim
The Korean Journal of Parasitology.2018; 56(6): 545. CrossRef
Plasmodium vivax msp-3α polymorphisms: analysis in the Indian subcontinent
Anju Verma, Hema Joshi, Vineeta Singh, Anup Anvikar, Neena Valecha
Malaria Journal.2016;[Epub] CrossRef

9,406 View
80 Download
7 Web of Science
Crossref

Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening: Yan Fu, Jingchao Lan, Xuhang Wu, Deying Yang, Zhihe Zhang, Huaming Nie, Rong Hou, Runhui Zhang, Wanpeng Zheng, Yue Xie, Ning Yan, Zhi Yang, Chengdong Wang, Li Luo, Li Liu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Guangyou Yang; Korean J Parasitol 2014;52(1):21-26.
Published online February 19, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.1.21

Abstract
PDF

Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.

Citations

Citations to this article as recorded by

Molecular Discovery of Filarial Nematode DNA in an Endangered Wild Pinniped (Galapagos Sea Lion, Zalophus wollebaeki)
Isabella G. Livingston, Taylor M. Gregory, Eleanor C. Hawkins, Ashley Cave, Andrea Loyola, Shelly L. Vaden, Diane Deresienski, Marjorie Riofrío‐Lazo, Gregory A. Lewbart, Diego Páez‐Rosas, Matthew Breen
Ecology and Evolution.2024;[Epub] CrossRef
Development of TaqMan Quantitative PCR Assays for Duplex Detection of Dirofilaria immitis COI and Dog GAPDH from Infected Dog Blood
In Young Oh, Kyung Tae Kim, Sun-Yeong Gwon, Ho Joong Sung
The Korean Journal of Clinical Laboratory Science.2019; 51(1): 64. CrossRef
Brown Spider (Loxosceles) Venom Toxins as Potential Biotools for the Development of Novel Therapeutics
Daniele Chaves-Moreira, Fernando Hitomi Matsubara, Zelinda Schemczssen-Graeff, Elidiana De Bona, Vanessa Ribeiro Heidemann, Clara Guerra-Duarte, Luiza Helena Gremski, Carlos Chávez-Olórtegui, Andrea Senff-Ribeiro, Olga Meiri Chaim, Raghuvir Krishnaswamy A
Toxins.2019; 11(6): 355. CrossRef
Development of Real-time PCR Assays for Detection ofDirofilaria immitisfrom Infected Dog Blood
In Young Oh, Kyung Tae Kim, Jin Hyun Jun, Jae-Ho Shin, Ho Joong Sung
Korean Journal of Clinical Laboratory Science.2016; 48(2): 88. CrossRef

9,399 View
84 Download
3 Web of Science
Crossref

Brief Communication

A Recombinant Matrix Metalloproteinase Protein from Gnathostoma spinigerum for Serodiagnosis of Neurognathostomiasis: Penchom Janwan, Pewpan M. Intapan, Hiroshi Yamasaki, Porntip Laummaunwai, Kittisak Sawanyawisuth, Chaisiri Wongkham, Chatchai Tayapiwatana, Amnat Kitkhuandee, Viraphong Lulitanond, Yukifumi Nawa, Wanchai Maleewong; Korean J Parasitol 2013;51(6):751-754.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.751

Abstract
PDF

Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components of molecular mass 21 kDa and 24 kDa in larval extracts of Gnathostoma spinigerum (Gs 21/24), has high sensitivity and specificity for diagnosis of neurognathostomiasis. However, only very small amounts of the Gs 21/24 antigens can be prepared from parasites harvested from natural or experimental animals. To overcome this problem, we recently produced a recombinant matrix metalloproteinase (rMMP) protein from G. spinigerum. In this study, we evaluated this rMMP alongside the Gs 21/24 antigens for serodiagnosis of human neurognathostomiasis. We studied sera from 40 patients from Srinagarind Hospital, Khon Kaen University, Thailand, with clinical criteria consistent with those of neurognathostomiasis, and sera from 30 healthy control adults from Thailand. All sera were tested for specific IgG antibodies against both G. spinigerum crude larval extract and rMMP protein using immunoblot analysis. The sensitivity and specificity for both antigenic preparations were all 100%. These results show that G. spinigerum rMMP protein can be used as an alternative diagnostic antigen, in place of larval extract, for serodiagnosis of neurognathostomiasis.

Citations

Citations to this article as recorded by

Protein and antigen profiles of third-stage larvae of Gnathostoma spinigerum assessed with next-generation sequencing transcriptomic information
Kathyleen Nogrado, Tipparat Thiangtrongjit, Poom Adisakwattana, Paron Dekumyoy, Sant Muangnoicharoen, Charin Thawornkuno, Onrapak Reamtong
Scientific Reports.2022;[Epub] CrossRef
Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis
Penchom Janwan, Pewpan M. Intapan, Lakkhana Sadaow, Rutchanee Rodpai, Hiroshi Yamasaki, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Phuangphaka Sadee, Wanchai Maleewong
Diagnostics.2021; 11(5): 862. CrossRef
Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development
Tipparat Thiangtrongjit, Kathyleen Nogrado, Thawatchai Ketboonlue, Preeyarat Malaitong, Poom Adisakwattana, Onrapak Reamtong
Pathogens.2021; 10(9): 1080. CrossRef
Combining lexical and context features for automatic ontology extension
Sara Althubaiti, Şenay Kafkas, Marwa Abdelhakim, Robert Hoehndorf
Journal of Biomedical Semantics.2020;[Epub] CrossRef
Human gnathostomiasis: a neglected food-borne zoonosis
Guo-Hua Liu, Miao-Miao Sun, Hany M. Elsheikha, Yi-Tian Fu, Hiromu Sugiyama, Katsuhiko Ando, Woon-Mok Sohn, Xing-Quan Zhu, Chaoqun Yao
Parasites & Vectors.2020;[Epub] CrossRef
Surveillance and diagnosis of zoonotic foodborne parasites
Reza Zolfaghari Emameh, Sami Purmonen, Antti Sukura, Seppo Parkkila
Food Science & Nutrition.2018; 6(1): 3. CrossRef

8,881 View
101 Download
Crossref

Original Article

Identification of Atg8 Isoform in Encysting Acanthamoeba: Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong; Korean J Parasitol 2013;51(5):497-502.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.497

Abstract
PDF

Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cyst-forming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess a type of Atg8. In this study, an isoform of Atg8 was identified and characterized in Acanthamoeba castellanii (AcAtg8b). AcAtg8b protein was found to encode 132 amino acids and to be longer than AcAtg8 protein, which encoded 117 amino acids. Real-time PCR analysis showed high expression levels of AcAtg8b and AcAtg8 during encystation. Fluorescence microscopy demonstrated that AcAtg8b is involved in the formation of the autophagosomal membrane. Chemically synthesized siRNA against AcAtg8b reduced the encystation efficiency of Acanthamoeba, confirming that AcAtg8b, like AcAtg8, is an essential component of cyst formation in Acanthamoeba. Our findings suggest that Acanthamoeba has doubled the number of Atg8 gene copies to ensure the successful encystation for survival when 1 copy is lost. These 2 types of Atg8 identified in Acanthamoeba provide important information regarding autophagy formation, encystation mechanism, and survival of primitive, cyst-forming protozoan parasites.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Efficacy of propolis extract and eye drop solutions to suppress encystation and excystation of Acanthamoeba triangularis WU19001-T4 genotype
Suthinee Sangkanu, Abolghasem Siyadatpanah, Roghayeh Norouzi, Julalak Chuprom, Watcharapong Mitsuwan, Sirirat Surinkaew, Rachasak Boonhok, Alok K. Paul, Tooba Mahboob, Imran Sama-ae, Sonia M. R. Oliveira, Tajudeen O. Jimoh, Maria de Lourdes Pereira, Polra
PeerJ.2024; 12: e16937. CrossRef
Ac-HSP20 regulates autophagy and promotes the encystation of Acanthamoeba castellanii by inhibiting the PI3K/AKT/mTOR signaling pathway
Siyao Guo, Di Liu, Xi Wan, Dingrui Guo, Meiyu Zheng, Wenyu Zheng, Xianmin Feng
Parasites & Vectors.2024;[Epub] CrossRef
Toxicity Evaluation of Potassium Sorbate In Vivo with Drosophila Melanogaster
Xubo Zhang, Qian Zhang, Xiaoxuan Song, Wanchen Yang, Andi Cheng, Jianzhen Zhang, Wei Dong
Insects.2024; 15(9): 703. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
The roles of autophagy and mitophagy in corneal pathology: current knowledge and future perspectives
Rajalakshmy Ayilam Ramachandran, Jose Marcos Sanches, Danielle M. Robertson
Frontiers in Medicine.2023;[Epub] CrossRef
Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation
Rachasak Boonhok, Suthinee Sangkanu, Suganya Phumjan, Ramita Jongboonjua, Nawarat Sangnopparat, Pattamaporn Kwankaew, Aman Tedasen, Chooi Ling Lim, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Karma G. Dolma, Alok
PeerJ.2022; 10: e13657. CrossRef
Proteomic analysis of Atg8-dependent recruitment of phagosomal proteins in the enteric protozoan parasite Entamoeba histolytica
Kumiko Nakada-Tsukui, Natsuki Watanabe, Kumiko Shibata, Ratna Wahyuni, Eri Miyamoto, Tomoyoshi Nozaki
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth
Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites
Hương-Giang Lê, Ji-Su Choi, Buyng-Su Hwang, Yong-Tae Jeong, Jung-Mi Kang, Tuấn-Cường Võ, Pyo-Yun Cho, Young-Kyung Lee, Won-Gi Yoo, Yeonchul Hong, Young-Taek Oh, Byoung-Kuk Na
Plants.2022; 11(24): 3459. CrossRef
Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level
Rachasak Boonhok, Suthinee Sangkanu, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Nurdina Charong, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Hazel
Parasitology.2021; 148(9): 1074. CrossRef
Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
Pathogens.2021; 10(7): 842. CrossRef
Ubiquitin-Like Modifiers: Emerging Regulators of Protozoan Parasites
Maryia Karpiyevich, Katerina Artavanis-Tsakonas
Biomolecules.2020; 10(10): 1403. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
Autophagy protein 12 plays an essential role in Acanthamoeba encystation
So-Hee Kim, Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong
Experimental Parasitology.2015; 159: 46. CrossRef
Autophagy Inhibitors as a Potential Antiamoebic Treatment for Acanthamoeba Keratitis
Eun-Kyung Moon, So-Hee Kim, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong
Antimicrobial Agents and Chemotherapy.2015; 59(7): 4020. CrossRef

9,825 View
99 Download
Crossref

Brief Communication

Effects of Mannose on Pathogenesis of Acanthamoeba castellanii: Kyung-Tae Yoo, Suk-Yul Jung; Korean J Parasitol 2012;50(4):365-369.
Published online November 26, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.4.365

Abstract
PDF

Acanthamoeba spp. are single-celled protozoan organisms that are widely distributed in the environment. In this study, to understand functional roles of a mannose-binding protein (MBP), Acanthamoeba castellanii was treated with methyl-alpha-D-mannopyranoside (mannose), and adhesion and cytotoxicity of the amoeba were analyzed. In addition, to understand the association of MBP for amoeba phagocytosis, phagocytosis assay was analyzed using non-pathogenic bacterium, Escherichia coli K12. Amoebae treated with mannose for 20 cycles exhibited larger vacuoles occupying the most area of the amoebic cytoplasm in comparison with the control group amoebae and glucose-treated amoebae. Mannose-selected amoebae exhibited lower levels of binding to Chinese hamster ovary (CHO) cells. Exogenous mannose inhibited >50% inhibition of amoebae (control group) binding to CHO cells. Moreover, exogenous mannose inhibited amoebae (i.e., man-treated) binding to CHO cells by <15%. Mannose-selected amoebae exhibited significantly decreased cytotoxicity to CHO cells compared with the control group amoebae, 25.1% vs 92.1%. In phagocytic assay, mannose-selected amoebae exhibited significant decreases in bacterial uptake in comparison with the control group, 0.019% vs 0.03% (P<0.05). Taken together, it is suggested that mannose-selected A. castellanii trophozoites should be severely damaged and do not well interact with a target cell via a lectin of MBP.

Citations

Citations to this article as recorded by

Epidemiology of and Genetic Factors Associated with Acanthamoeba Keratitis
Muhammad Ilyas, Fiona Stapleton, Mark D. P. Willcox, Fiona Henriquez, Hari Kumar Peguda, Binod Rayamajhee, Tasbiha Zahid, Constantinos Petsoglou, Nicole A. Carnt
Pathogens.2024; 13(2): 142. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Commensals Serve as Natural Barriers to Mammalian Cells during Acanthamoeba castellanii Invasion
Yu-Jen Wang, Chun-Hsien Chen, Jenn-Wei Chen, Wei-Chen Lin, Kevin R. Theis
Microbiology Spectrum.2021;[Epub] CrossRef
Lectins as Virulence Factors in Entamoeba Histolytica and Free-Living Amoebae
Paula Guzmán-Téllez, Moisés Martínez-Castillo, Nadia Flores-Huerta, Gabriela Rosales-Morgan, Judith Pacheco-Yépez, Mireya de la Garza, Jesús Serrano-Luna, Mineko Shibayama
Future Microbiology.2020; 15(10): 919. CrossRef
Acanthamoeba castellanii as an alternative interaction model for the dermatophyte Trichophyton rubrum
Lucas V. de Faria, Paulo H. F. do Carmo, Marliete C. da Costa, Nalu T. A. Peres, Isabela A. Rodrigues Chagas, Cinthia Furst, Gabriella F. Ferreira, Adriana O. Costa, Daniel A. Santos
Mycoses.2020; 63(12): 1331. CrossRef
Interactions Between Acanthamoeba culbertsoni and Pathogenic Bacteria and their Inhibition by Lectin-Antibodies
Suk-Yul Jung
Journal of Pure and Applied Microbiology.2020; 14(3): 1687. CrossRef
Tupanvirus-infected amoebas are induced to aggregate with uninfected cells promoting viral dissemination
Graziele Oliveira, Lorena Silva, Thiago Leão, Said Mougari, Flávio Guimarães da Fonseca, Erna Geessien Kroon, Bernard La Scola, Jônatas Santos Abrahão
Scientific Reports.2019;[Epub] CrossRef
Production of a monoclonal antibody against a mannose-binding protein of Acanthamoeba culbertsoni and its localization
A-Young Kang, A-Young Park, Ho-Joon Shin, Naveed Ahmed Khan, Sutherland K. Maciver, Suk-Yul Jung
Experimental Parasitology.2018; 192: 19. CrossRef
Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression
Sook-Luan Ng, Anisah Nordin, Norzana Abd Ghafar, Yusof Suboh, Noraina Ab Rahim, Kien-Hui Chua
Parasites & Vectors.2017;[Epub] CrossRef

8,358 View
103 Download
Crossref

Case Report

An Indigenous Case of Intestinal Capillariasis with Protein-Losing Enteropathy in Korea: Woon Tae Jung, Hyun Jin Kim, Hyun Ju Min, Chang Yoon Ha, Hong Jun Kim, Gyung Hyuck Ko, Byoung-Kuk Na, Woon-Mok Sohn; Korean J Parasitol 2012;50(4):333-337.
Published online November 26, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.4.333

Abstract
PDF

We encountered an indigenous case of intestinal capillariasis with protein-losing enteropathy in the Republic of Korea. A 37-year-old man, residing in Sacheon-si, Gyeongsangnam-do, admitted to the Gyeongsang National University Hospital (GNUH) due to long-lasting diarrhea, abdominal pain, anasarca, and weight loss. He recalled that he frequently ate raw fish, especially the common blackish goby (Acanthogobius flavimanus) and has never been abroad. Under the suspicion of protein-losing enteropathy, he received various kinds of medical examinations, and was diagnosed as intestinal capillariasis based on characteristic sectional findings of nematode worms in the biopsied small intestine. Adults, juvenile worms, and eggs were also detected in the diarrheic stools collected before and after medication. The clinical symptoms became much better after treatment with albendazole 400 mg daily for 3 days, and all findings were in normal range in laboratory examinations performed after 1 month. The present study is the 6th Korean case of intestinal capillariasis and the 3rd indigenous one in the Republic of Korea.

Citations

Citations to this article as recorded by

Imported parasitic diseases in the Republic of Korea: status and issues
Jong-Yil Chai
Journal of the Korean Medical Association.2025; 68(1): 52. CrossRef
Human parasitic infections of the class Adenophorea: global epidemiology, pathogenesis, prevention and control
Jitrawadee Intirach, Chang Shu, Xin Lv, Suzhen Gao, Nataya Sutthanont, Tao Chen, Zhiyue Lv
Infectious Diseases of Poverty.2024;[Epub] CrossRef
Paleoparasitology research on ancient helminth eggs and larvae in the Republic of Korea
Jong-Yil Chai, Min Seo, Dong Hoon Shin
Parasites, Hosts and Diseases.2023; 61(4): 345. CrossRef
An Overview of Fish-borne Nematodiases among Returned Travelers for Recent 25 Years– Unexpected Diseases Sometimes Far Away from the Origin
Jorge Costa Eiras, Gilberto Cezar Pavanelli, Ricardo Massato Takemoto, Yukifumi Nawa
The Korean Journal of Parasitology.2018; 56(3): 215. CrossRef
Intestinal capillariasis in the 21stcentury: clinical presentations and role of endoscopy and imaging
Julajak Limsrivilai, Supot Pongprasobchai, Piyaporn Apisarnthanarak, Sathaporn Manatsathit
BMC Gastroenterology.2014;[Epub] CrossRef
Intestinal Capillariasis Diagnosed by Endoscopic Biopsy
Minsu Ha, Dong-Ha Jun, Jung Ho Kim, Yoon Jae Kim, Kwang An Kwon, Dong Kyun Park, Yang Suh Ku
Clinical Endoscopy.2013; 46(6): 675. CrossRef

9,914 View
89 Download
Crossref

Brief Communication

Depressed Neuronal Growth Associated Protein (GAP)-43 Expression in the Small Intestines of Mice Experimentally Infected with Neodiplostomum seoulense: Kyoung-Ho Pyo, Eun-Young Kang, Bong-Kwang Jung, Jung-Ho Moon, Jong-Yil Chai, Eun-Hee Shin; Korean J Parasitol 2012;50(1):89-93.
Published online March 6, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.1.89

Abstract
PDF

Neodiplostomum seoulense (Digenea: Neodiplostomidae) is an intestinal trematode that can cause severe mucosal pathology in the small intestines of mice and even mortality of the infected mice within 28 days after infection. We observed neuronal growth associated protein-43 (GAP-43) expression in the myenteric plexus of the small intestinal wall of N. seoulense-infected mice until day 35 post-infection (PI). BALB/c mice were infected with 200 or 500 N. seoulense metacercariae isolated from naturally infected snakes and were killed every 7 days for immunohistochemical demonstration of GAP-43 in the small intestines. N. seoulense-infected mice showed remarkable dilatation of intestinal loops compared with control mice through days 7-28 PI. Conversely, GAP-43 expression in the mucosal myenteric plexus was markedly (P<0.05) reduced in the small intestines of N. seoulense-infected mice during days 7-28 PI and was slightly normalized at day 35 PI. From this study, it is evident that neuronal damage occurs in the intestinal mucosa of N. seoulense-infected mice. However, the correlation between intestinal pathology, including the loop dilatation, and depressed GAP-43 expression remains to be elucidated.

Citations

Citations to this article as recorded by

Enhanced serotonin signaling increases intestinal neuroplasticity
Chasen J. Greig, Neeru Gandotra, John J. Tackett, Michaela C. Bamdad, Robert A. Cowles
Journal of Surgical Research.2016; 206(1): 151. CrossRef